Antigen-binding molecule capable of binding to two or more antigen molecules repeatedly

A method of screening for an antibody, the method comprising:n(a) assaying the antigen-binding activity of an antibody at pH 6.7 to pH 10.0;(b) assaying the antigen-binding activity of the antibody at pH 4.0 to pH 6.5;(c) determining that the antibody's antigen-binding activity at pH 6.7 to pH 10.0 is greater than its antigen-binding activity at pH 4.0 to pH 6.5;(d) selecting the antibody based on the determination in (c); and(e) assaying the retention in plasma of the antibody selected in step (d).

A method of screening for an antibody, the method comprising:n(a) assaying the antigen-binding activity of an antibody at pH 6.7 to pH 10.0;(b) assaying the antigen-binding activity of the antibody at pH 4.0 to pH 6.5;(c) determining that the antibody's antigen-binding activity at pH 6.7 to pH 10.0 is greater than its antigen-binding activity at pH 4.0 to pH 6.5;(d) selecting the antibody based on the determination in (c); and(e) assaying the ability of the antibody selected in step (d) to eliminate an antigen in plasma.

A method of screening for an antibody, the method comprising:n(a) binding an antibody to an antigen at pH 6.7 to pH 10.0;(b) placing the antibody bound to the antigen of (a) under a condition of pH 4.0 to pH 6.5;(c) determining that the antibody has dissociated from the antigen under the condition of pH 4.0 to pH 6.5;(d) selecting the antibody based on the determination in (c); and(e) assaying the retention in plasma of the antibody obtained in step (d).

A method of screening for an antibody, the method comprising:n(a) binding an antibody to an antigen at pH 6.7 to pH 10.0;(b) placing the antibody bound to the antigen of (a) under a condition of pH 4.0 to pH 6.5;(c) determining that the antibody has dissociated from the antigen under the condition of pH 4.0 to pH 6.5;(d) selecting the antibody based on the determination in (c); and(e) assaying the ability of the antibody in step (d) to eliminate an antigen in plasma.

A method of screening for an antibody whose binding activity at a first pH is greater than that at a second pH, the method comprising:n(a) binding an antibody to an antigen-immobilized column at a first pH;(b) eluting the antibody from the column at a second pH;(c) collecting the eluted antibody, wherein the first pH is 6.7 to 10.0 and the second pH is 4.0 to 6.5, and wherein the antibody eluted from the column because its binding to the antigen at the first pH is greater than its binding to the antigen at the second pH; and(d) assaying the retention in plasma of the antibody collected in step (c).

A method of screening for an antibody whose binding activity at a first pH is greater than that at a second pH, the method comprising:n(a) binding an antibody to an antigen-immobilized column at a first pH;(b) eluting the antibody from the column at a second pH;(c) collecting the eluted antibody, wherein the first pH is 6.7 to 10.0 and the second pH is 4.0 to 6.5, and wherein the antibody eluted from the column because its binding to the antigen at the first pH is greater than its binding to the antigen at the second pH; and(d) assaying the ability of the antibody collected in step (c) to eliminate an antigen in plasma.

A method of screening for an antibody whose binding activity at a first pH is greater than that at a second pH, the method comprising:n(a) binding an antibody library to an antigen-immobilized column at a first pH that is pH 6.7 to 10.0;(b) eluting an antibody from the column at a second pH that is pH 4.0 to 6.5;(c) amplifying nucleic acid encoding the eluted antibody;(d) obtaining the nucleotide sequence of the nucleic acid encoding the eluted antibody;(e) determining the amino acid sequence encoded by the nucleic acid; and(f) producing an antibody with the amino acid sequence determined in step (e) and assaying the retention in plasma of the produced antibody.

A method of screening for an antibody whose binding activity at a first pH is greater than that at a second pH, the method comprising:n(a) binding an antibody library to an antigen-immobilized column at a first pH that is pH 6.7 to 10.0;(b) eluting an antibody from the column at a second pH that is pH 4.0 to 6.5;(c) amplifying nucleic acid encoding the eluted antibody;(d) obtaining the nucleotide sequence of the nucleic acid encoding the eluted antibody;(e) determining the amino acid sequence encoded by the nucleic acid; and(f) producing an antibody with the amino acid sequence determined in step (e) and assaying the ability of the produced antibody to eliminate an antigen in plasma.