The present invention relates to novel vaccines for the prevention or attenuation of infection by Streptococcus pneumoniae. The invention further relates to isolated nucleic acid molecules encoding antigenic polypeptides of Streptococcus pneumoniae. Antigenic polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. The invention additionally relates to diagnostic methods for detecting Streptococcus nucleic acids, polypeptides and antibodies in a biological sample.

Claims

73 · 2 independent · depth 4

73 granted claims

›1.independentAn isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of: (a) a polynucleotide encoding amino acids 1 to 79…+40

›2.↳ 1The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (a).d2+1

›3.↳ 1The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (b).d2

›4.↳ 1The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (c).d2+22

›11.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Arg-10 to Arg-17 of SEQ ID NO:56.d3

›12.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Lys-29 to Ser-39 of SEQ ID NO:56.d3

›13.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Ser-140 to Ala-153 of SEQ ID NO:56.d3

›14.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Arg-158 to Tyr-169 of SEQ ID NO:56.d3

›15.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Asp-175 to Ala-183 of SEQ ID NO:56.d3

›16.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Gly-216 to Asn-236 of SEQ ID NO:56.d3

›17.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Ala-261 to Leu-270 of SEQ ID NO:56.d3

›18.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Arg-282 to Phe-291 of SEQ ID NO:56.d3

›19.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Thr-297 to Ala-305 of SEQ ID NO:56.d3

›20.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Pro-342 to Gln-362 of SEQ ID NO:56.d3

›21.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Phe-455 to Asp-463 of SEQ ID NO:56.d3

›22.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues His-497 to Thr-511 of SEQ ID NO:56.d3

›23.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Ala-521 to Gly-529 of SEQ ID NO:56.d3

›24.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Ile-537 to Val-546 of SEQ ID NO:56.d3

›25.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Ile-556 to Ala-568 of SEQ ID NO:56.d3

›26.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Pro-581 to Ser-595 of SEQ ID NO:56.d3

›27.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Glu-670 to Ala-685 of SEQ ID NO:56.d3

›28.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Ser-696 to Ala-705 of SEQ ID NO:56.d3

›29.↳ 4The isolated nucleic acid molecule of claim 4, wherein said epitope-bearing portion comprises amino residues Leu-782 to Ser-791 of SEQ ID NO:56.d3

›30.↳ 4A method of producing a polypeptide comprising: (a) culturing a host cell containing the polynucleotide of claim 4 under conditions suitable to produc…d3+2

›5.↳ 1The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (d).d2+1

›7.↳ 1The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (e).d2+1

›9.↳ 1The isolated nucleic acid molecule of claim 1, wherein said polynucleotide is (f).d2

›33.↳ 1The isolated nucleic acid molecule of claim 1, wherein said nucleic acid molecule comprises a heterologous polynucleotide sequence.d2+1

›35.↳ 1A method for making a recombinant vector comprising inserting the isolated nucleic acid molecule of claim 1 into a vector.d2

›36.↳ 1A recombinant vector comprising the isolated nucleic acid molecule of claim 1.d2+1

›38.↳ 1A recombinant host cell comprising the isolated nucleic acid molecule of claim 1.d2+1

›40.↳ 1A method of using the polynucleotide of claim 1 to detect a nucleic acid molecule which hybridizes to said polynucleotide in a biological sample compr…d2

›41.↳ 1A method of detecting Streptococcus nucleic acids in a biological sample obtained from an animal comprising: (a) contacting the biological sample with…d2

›42.independentAn isolated polypeptide comprising a member selected from the group consisting of: (a) a polypeptide of amino acid residues 1 to 796 of SEQ ID NO:56; …+31

›43.↳ 42The isolated polypeptide of claim 42, wherein said polypeptide is (c).d2+19

›44.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Arg-10 to Arg-17 of SEQ ID NO:56.d3

›45.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Lys-29 to Ser-39 of SEQ ID NO:56.d3

›46.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Ser-140 to Ala-153 of SEQ ID NO:56.d3

›47.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Arg-158 to Tyr-169 of SEQ ID NO:56.d3

›48.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Asp-175 to Ala-183 of SEQ ID NO:56.d3

›49.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Gly-216 to Asn-236 of SEQ ID NO:56.d3

›50.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Ala-261 to Leu-270 of SEQ ID NO:56.d3

›51.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Arg-282 to Phe-291 of SEQ ID NO:56.d3

›52.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Thr-297 to Ala-305 of SEQ ID NO:56.d3

›53.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Pro-342 to Gln-362 of SEQ ID NO:56.d3

›54.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Phe-455 to Asp-463 of SEQ ID NO:56.d3

›55.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues His-497 to Thr-511 of SEQ ID NO:56.d3

›56.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Ala-521 to Gly-529 of SEQ ID NO:56.d3

›57.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Ile-537 to Val-546 of SEQ ID NO:56.d3

›58.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Ile-556 to Ala-568 of SEQ ID NO:56.d3

›59.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Pro-581 to Ser-595 of SEQ ID NO:56.d3

›60.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Glu-670 to Ala-685 of SEQ ID NO:56.d3

›61.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Ser-696 to Ala-705 of SEQ ID NO:56.d3

›62.↳ 43The isolated polypeptide of claim 43, wherein said epitope-bearing portion comprises amino residues Leu-782 to Ser-791 of SEQ ID NO:56.d3

›63.↳ 42The isolated polypeptide of claim 42, wherein said polypeptide is (b).d2

›64.↳ 42The isolated polypeptide of claim 42, wherein said polypeptide is fused to a heterologous polypeptide.d2

›65.↳ 42A method of producing the polypeptide of claim 42 comprising: (a) culturing a host cell capable of expressing said polypeptide under conditions suitab…d2+2

›67.↳ 42The isolated polypeptide of claim 42, wherein said polypeptide is (a).d2+1

›69.↳ 42A method of attenuating an infection caused by a member of the Streptococcus genus in an animal, comprising administering to said animal the polypepti…d2+3

Classifications

17 codes

USPC · US Patent Classification

424/165.1536/25.3435/69.3435/388.4435/7.34435/252.3424/200.1536/24.3435/36536/24.32424/244.1424/184.1435/340424/165.1435/253.4435/7.2424/197.11

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James C. Housel

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