Pyridazine derivatives, manufacturing method and related composition

This application claims priority from Japanese Patent application No. 11-356201, filed Dec. 15, 1999, which is incorporated herein by reference.

The present invention relates to pyridazine derivatives, a method for its manufacture, compositions thereof including ultraviolet absorbents and photostabilizers, ultraviolet-ray-absorptive compositions and external preparations for skin.

Ultraviolet rays of wavelength 290 nm or less in sunlight are absorbed by the ozone layer. Accordingly, these do not reach the surface of the earth. However, as the ultraviolet rays of 290 to 400 nm reach the surface of the earth, these ultraviolet rays have various effects. In skin chemistry, the ultraviolet rays of the wavelength of 290 nm to 320 nm cause the formation of erythema and blistering. It is known that these ultraviolet rays cause acceleration of melanism and chromatosis. The long wavelength ultraviolet rays of 320 to 400 nm causes the melanism of skin immediately after irradiation. Also, since the energy reaches to an corium of skin, these ultraviolet rays influence the elastica in the walls of blood vessels and connective tissue. These ultraviolet rays of middle wavelength to long wavelength accelerate the aging of a skin. Also, it is thought that these ultraviolet rays are a cause of the formation of stains, freckles, wrinkles and the like.

To protect the skin from such ultraviolet rays, ultraviolet absorbents have been used. These ultraviolet absorbents include, for example, benzotriazole derivatives, benzophenone derivatives, salicylic acid derivatives, p-aminobenzoic acid derivatives, cinnamic acid derivatives, and urocanic acid derivatives.

These ultraviolet absorbents are used in photostabilizers of colorant, perfume, drug, etc., in medical supplies and cosmetics.

Also, ultraviolet absorbents are used in fields other than medical supplies and cosmetics. For example, they are added to the various materials of coating, dye, pigment, resin, synthetic rubber, latex, film and fiber. As these are given absorbing ability for ultraviolet rays, a product, or paints or films coating a product can be protected from the ultraviolet rays. The ultraviolet absorbent is used to maintain quality by preventing degradation, degeneration and so on by the ultraviolet rays.

It is desirable that an ultraviolet absorbent is able to absorb the ultraviolet rays of all the wavelength range of 290 nm to 400 nm which reach the surface of the earth. Also, when an ultraviolet absorbent is included in an external preparation for skin, it is important that the ultraviolet absorbent is not decomposed by sunlight exposure. Also, it is important that the ultraviolet absorbent does not cause skin irritation.

However, conventional ultraviolet absorbents do not always satisfy these preferences. Conventional ultraviolet absorbents sometimes cause coloring and deposition due to ultraviolet rays shielding agents in inorganic powders commonly used in external preparations for skin. Also, a satisfactory photostabilizer compound has been needed.

Also, conventional ultraviolet rays absorbents in other fields sublimate and volatilize by heating during sintering of paints and in the forming of resin. In addition, these absorbents vaporize gradually and become less effective with the passing of time, even if it is not heated.

The present invention is achieved in view of the foregoing prior art. The object of the present invention is to provide an ultraviolet absorbent, a photostabilizer and a manufacturing method, which have an excellent absorbing ability in the wide ultraviolet rays wavelength range, that have high stability and safety. It is a another object of the present invention to provide an ultraviolet ray absorption composition including said ultraviolet absorbent. It is further object of the present invention to provide an external preparation for skin including said ultraviolet absorbent or said photostabilizer.

As a result of diligent study by the present inventors, it was found that a certain kind of pyridazine derivatives have the above-mentioned properties and are excellent ultraviolet absorbents and photostabilizers.

Namely, the present invention is pyridazine derivatives of general formula (1) and salts thereof. The compound of the present invention has excellent absorbing ability with respect to the wide ultraviolet ray wavelength range. As it is very stable and safe, it is an excellent ultraviolet absorbent and photostabilizer.

A manufacturing method of the pyridazine derivatives comprises the process of reacting at least 10 wt % of 4,5-Dichloro-3-hydroxypyridazine or 4,5-Dibromo-3-hydroxypyridazine or combination thereof, with at least 20 vol % of morpholine in a reaction solution at 70° C. or higher.

An ultraviolet absorbent of the present invention comprises said pyridazine derivatives and/or salts thereof as an active ingredient.

An ultraviolet ray absorption composition of the present invention includes said ultraviolet absorbents.

A photostabilizer of the present invention comprises said pyridazine derivatives and/or salts thereof, as an active ingredient. It is preferable that said photostabilizer includes a sequestering agent.

An external preparation for skin of the present invention comprises said ultraviolet absorbents. Also, it is preferable that the external preparation for skin of the present invention includes an inorganic powder.

Also, an external preparation for skin of the present invention comprises said photostabilizer. It is preferable that said external skin preparation includes a sequestering agent.

Also, in the external skin preparation of the present invention, it is preferable that said external preparation for skin includes 0.001 wt % to 20 wt % of said pyridazine derivatives or salts thereof.

FIG. 1 shows the ultraviolet absorption spectrum of pyridazine derivative (4,5-Dimorpholino-3-hydroxypyridazine) of the present invention.

Pyridazine Derivatives And Salts Thereof

A pyridazine derivative of the present invention is shown in a general formula (1). This compound can be isomerized to general formula (1′), which is tautomer with the equilibrium like the following, under certain conditions.

The pyridazine derivatives in the present invention are described only by the general formula (1) for convenience. However, the pyridazine derivatives in the present invention can be isomerized to a general formula (1′) as a tautomer.

The chemical name of the pyridazine derivative of the present invention includes 4,5-Dimorpholino-3-hydroxypyridazine and 4,5-Dimorpholino-3-hydroxypyridazine hydrochloride and the like.

A pyridazine derivative of the present invention can be synthesized by the followings method.

In the above-mentioned reaction formula, A represents a chlorine atom or bromine atom. The compound of a general formula (3) (when A is a chlorine atom, 4,5-Dichloro-3-hydroxypyridazine; when A is a bromine atom, 4,5-Dibromo-3-hydroxypyridazine) can be synthesized by the method of Chemische Berichte, 32, 543(1899) and so on in accordance with the above-mentioned formula. The compounds of the general formula (2) can be easily available. Namely, the compounds of a general formula (3) is easily obtained by cyclic reaction of compounds of a general formula (2) and hydrazine. Also, the compounds of the general formula (3) (A is chlorine atom) which can be available from ALDRICH Inc. Also, pyridazine derivatives of the present invention were obtained by reacting 10 wt % or more of a compound of the general formula (3) and 20 vol % or more of morpholine in a reaction solution at 70° C. or higher. In the case where the concentration of compounds of the general formula (3) in the reaction solution is less than 10 wt %, in the case where the concentration of morpholine in the reaction solution is less than 20 vol %, and in the case where reaction temperature is lower than 70° C., it was difficult to obtain pyridazine derivatives of the present invention.

Also, the pyridazine derivatives of the present invention include inorganic acid salt or organic acid salt made by published methods. Examples of inorganic acids include hydrochloric acid, sulfuric acid, phosphoric acid, hydrobromic acid. Examples of organic acids include acetic acid, lactic acid, maleic acid, fumaric acid, tartaric acid, citric acid, methanesulfonic acid, p-toluenesulfonic acid.

Ultraviolet Absorbent And External Preparation For Skin

An ultraviolet absorbent having as its principal component pyridazine derivative or salt thereof can be included in various products. An external skin preparation including this absorbent is suitable. An external preparation for skin having the ultraviolet absorbent of the present invention demonstrates an excellent ultraviolet ray prevention effect. Also, since the ultraviolet absorbent does not decompose under sunlight exposure, the effect is continued for a long time. Also, it does not cause problems for the skin. Accordingly, it is especially useful as the external skin preparation for sun screen.

Also, to increase the ultraviolet rays shielding effect in an external skin preparation for sun screen, it is preferred that an ultraviolet absorbent of an organic compound and an ultraviolet ray shielding agent of an inorganic powder are included. Also, many cosmetics for makeup include inorganic powder. However, use of an organic ultraviolet absorbent and inorganic powder may cause discoloration.

The ultraviolet absorbent of the present invention does not cause discoloration, when included with an inorganic powder in an external skin preparation for skin. Therefore, it is possible to include inorganic powder.

Inorganic Powder

In the present invention, an inorganic powder includes powder in cosmetics and medical supplies. Examples of inorganic powder include talc, kaolin, boron nitride, mica, sericite, muscovite, black mica, golden mica, synthetic mica, vermiculite, magnesium carbonate, calcium carbonate, silicic anhydride, aluminum silicate, aluminum oxide, barium silicate, calcium silicate, magnesium cilicate, tungsten metal salt, magnesium, silica, zeolite, barium sulfate, calcined calcium sulfate, calcined gypsum, calcium phosphate, fluoroapatite, calcium phosphate hydroxide, ceramic powder, metal soap (zinc myristate, calcium palmitate, aluminum stearate etc.). Also, examples of inorganic pigment include titanium dioxide, zinc oxide, iron oxide, iron titanium oxide, carbon, low-valent titanium oxide, mango violet, cobalt violet, chromium oxide, chromium hydroxide, cobalt titanium oxide, ultramarine, iron blue, titanium oxide coated mica, titanium oxide coated bismuth oxychloride, colored titanium oxide coated mica, bismuth oxychloride, fish scale flake.

Photostabilizer

The pyridazine derivatives and salts thereof of the present invention are useful as a photostabilizer. Especially, the compound is an excellent photostabilizer of colorants, perfumes and drugs in medical supplies and cosmetics. Also, the pyridazine derivatives and salts thereof of the present invention can achieve a synergistic photostabilization effect, by including a sequestering agent.

Sequestering Agent

In the present invention, pyridazine derivatives or salts thereof can be used with a sequestering agent. Examples of sequestering agents include sodium ethylenediaminetetraacetate (EDTA), trisodium hydroxyethyl ethylenediamine triacetate(dihydrate), phosphoric acid, citric acid, ascorbic acid, succinic acid, gluconic acid, sodium polyphosphate, sodium metaphosphate, 1-hydroxyethane 1,1-diphosphate.

Use Of External Preparation For Skin

The external preparation for skin of the present invention may includes the above-mentioned ultraviolet absorbent or the above-mentioned photostabilizer. Forms of the external preparation for skin of the present invention are not restricted if the effect of the present invention is demonstrated. Examples of forms of the external preparation for skin of the present invention include lotion, milky lotion, cream and essence for skin care cosmetics. Also, examples of makeup cosmetics include base cosmetics, foundation, lipstick, face color and eyeliner. Also, examples of cosmetics for hair and scalp include hair spray, hair tonic and hair liquid.

Amount Of Pyridazine Derivative Or Salts Thereof In An External Skin Preparation

When the external preparation for skin includes the pyridazine derivatives and/or salts thereof of the present invention, the amount depends on the need for UV absorbing ability or photostabilization ability. Usually the preferable amount of pyridazine derivative and/or salt thereof in a composition is 0.001 wt % to 20 wt %, more preferably 0.01 wt % to 10 wt %. If the amount is less than 0.001 wt %, the ultraviolet rays prevention effect or photostabilization effect may inadequate. Also, if the amount is more than 20 wt %, it may be difficult to maintain the form of external skin preparation.

Other Ingredients

The external preparation for skin of the present invention can include other ingredients often included in cosmetics and medical supplies. Examples of other ingredients include liquid fat and oil, solid fat and oil, wax, hydrocarbon, higher fatty acid, higher alcohol, ester, silicone, anionic surfactant, cationic surfactant, ampholytic surfactants, nonionic surfactant, humectants, water-soluble high molecular compounds, thickeners, film formers, lower alcohol, polyhydric alcohol, saccharides, amino acid, organic amine, pH adjustment agent, skin nutrition agents, vitamins, antioxidants, perfumes, powder, colorants and water and the like. These ingredients can be combined in external preparation for skin of the present invention if needed. Also, ultraviolet absorbents and photostabilizers other than the pyridazine derivatives of the present invention can be combined unless the objects of the invention are thwarted.

Ultraviolet Absorptive Composition

The ultraviolet absorbent of the present invention can be used in products other than external skin preparation. For example, coating, dye, pigment, resin, synthetic rubber, latex, film, fiber and so on can include the ultraviolet absorbent of the present invention for ultraviolet ray prevention. Since pyridazine derivatives of the present invention excel in heat stability without vaporizing, the effect can be maintained for a long time. The preferable amount in this case is usually 0.00 wt % to 20 wt %, more preferably 0.01 wt % to 10 wt %. If the amount is less than 0.001 wt %, the ultraviolet ray defense effect may be inadequate. If the amount is greater than 20 wt %, it may be difficult to form of the external skin preparation.

The present invention is explained in more fully by the following examples, but, the present invention is not restricted to these examples. The following are the manufacturing examples of pyridazine derivatives of the present invention.

1 A method synthesizing a 4,5-Dimorphorino-3-hydroxypyridazine

4,5-Dichloro-3-hydroxypyridazine (25.0 g, 0.151 mol=about 17 wt % in reaction solusion) was dissolved to morpholine (120 ml=100 vol %). The mixture was heated at 70° C. or more for 24 hours. After being cooled, deposited crystal was filtered. White crystal of 4,5-Dimorpholibo-3-hydroxypyridazine (37.2 g, yield percentage 92%) was obtained.

Melting point 256 to 257° C. (Decomposition) (Capil.)

Next, chemical analysis values of the obtained compound were shown. Table 1 shows the result of elemental analysis. Next, the results of 1 H—NMR, 13 C—NMR and MS spectra were shown. These chemical data support the desired compound.

1 H—NMR(DMSO—d 6 , TMS, ppm)

δ: 3.21 (dd, 4H, J=4.4&4.9Hz: —CH 2 —N—CH 2 —),

3.23 (dd, 4H, J=4.4&4.9Hz: —CH 2 —N—CH 2 —),

3.62 (dd, 4H, J=4.4&4.9Hz: —CH 2 —O—CH 2 —),

3.70 (dd, 4H, J=4.4&4.9Hz: —CH 2 —O—CH 2 —),

7.67 (s, 1H: pyridazine ring H-6), 12.38 (s, 1H: OH)

13 C—NMR: (DMSO—d 6 , TMS, ppm)

δ: 47.8 (—CH 2 —N—CH 2 —), 48.5 (—CH 2 —N—CH 2 —), 66.1 (—CH 2 —O—CH 2 —), 66.6(—CH 2 —O—CH 2 —), 131.1, 132.6, 141.0(pyridazine ring C-4, C-5, C-6), 160.7 (pyridazine ring C-3)

MS spectrum: MW=266(C 12 H 18 N 4 O 3 =266.30)

2 A method synthesizing a 4,5-Dimorpholino-3-hydroxyPyridazine

4,5-Dibromo-3-hydroxypyridazine (25.0 g, 0.098 mol=about 17 wt % in reaction solution) was dissolved to morpholine (120 ml=100 vol %). The mixture was heated at 70° C. or higher for 24 hours. After being cooled, deposited crystal was filtered. White crystal of 4,5-Dimorpholibo-3-hydroxypyridazine (23.7 g, yield percentage 90%) was obtained.

Melting point 256 to 257° C. (Decomposition) (Capil.)

Next, chemical analysis values for the obtained compound are shown. Table 2 shows the result of elementary analysis. Next, the results of 1 H—NMR, 13 C—NMR and MS spectra were shown. These chemical data support the desired components.

1 H—NMR(DMSO—d 6 , TMS, ppm)

δ: 3.21 (dd, 4H, J=4.4&4.9 Hz: —CH 2 —N—CH 2 —),

3.23 (dd, 4H, J=4.4&4.9Hz: —CH 2 —N—CH 2 —),

3.62 (dd, 4H, J=4.4&4.9 Hz: —CH 2 —O—CH 2 —),

3.70 (dd, 4H, J=4.4&4.9 Hz: —CH 2 —O—CH 2 —),

7.67 (s, 1H: pyridazine ring H-6), 12.38(s, 1H:OH)

13 C—NMR: (DMSO—d 6 , TMS, ppm)

δ: 47.8 (—CH 2 —N—CH 2 —), 48.5(—CH 2 —N—CH 2 —), 66.1 (—CH 2 —O—CH 2 —),

66.6 (—CH 2 —O—CH 2 —), 131.1, 132.6,

141 . 0 (pyridazine ring C-4, C-5, C-6),

160.7 (pyridazine ring C-3)

MS spectrum: MW=266(C 12 H 18 N 4 O 3 =266.30)

Next, test result for ultraviolet rays absorbing ability of the pyridazine derivatives of the present invention are shown.

Absorption

Ultraviolet rays absorption spectrum of 4,5-Dimorpholino-3-hydroxypyridazine (Solvent: water, Concentration: 10 ppm, Light path: 1 cm) was measured by the spectrophotometer (Manufactured by Nihonbunko Inc., Trade name: Ubest-55). The result was shown in FIG. 1 .

FIG. 1 shows that a pyridazine derivative of the present invention can absorb strongly with respect to the entire wavelength range of ultraviolet rays, 290 nm to 400 nm, which reach the surface of the earth. Also, it shows hardly any absorption in visible range for wavelengths longer than 400 nm. Accordingly, pyridazine derivatives of the present invention is excellent in visual transparency.

Test Example 2

Ultraviolet Rays Prevention Effect

(i) Test method

The prevention effect test was carried out on a beach during the summer. Equal amounts of sample were applied to the right and left sides of the backs of test subjects. After direct sunlight exposure, the degree of sunburn was evaluated in accordance with the following criteria. Each group consisted of 20 subjects.

(Criterion)

Remarkable effect: None or almost no sunburn symptom was found.

Positive effect: Slight sunburn symptom was found.

Negative effect: Strong sunburn symptom was found.

(Evaluation)

A: Subject of remarkable effect or positive effect is 80% or more.

B: Subject of remarkable effect or positive effect is 50% or more and less than 80%.

C: Subject of remarkable effect or positive effect is 30% or more and less than 50%.

D: Subject of remarkable effect or positive effect is less than 30%

(ii) Preparation of sample

(A) Lotion

Each of water phase and alcohol phase was prepared. Then each was mixed.

(B) Cream

The propylene glycol was added to ion-exchanged water and was dissolved, which was kept at 70° C. by heating (Water phase). The other components were mixed and melted by heating and was kept at 70° C. (Oil phase). The oil phase was added to the water phase, and an emulsion was formed.. After it was homogeneously emulsified with a homogenixer, it was cooled at 30° C. with stirring well.

(iii) Result

The result with regard to lotion (a), cream (b) were shown in Table 3 and 4, respectively.

Table 3 and Table 4 show that external skin preparation including a pyridazine derivative of the present ultraviolet ray prevention effect as an ultraviolet absorbent. Also, it shows that the preferable amount of pyridazine derivative and/or salt thereof of the present invention is 0.001 wt % to 20 wt %. Also, having an amount greater than 20 wt % makes it difficult to form an external skin preparation.

Accordingly, the pyridazine derivatives of the present invention have excellent absorbing ability with regard to wide range of ultraviolet rays. The inventors have studied with regard to the amount of the present invention in an ultraviolet absorbent in external skin preparation. The inventors have studied it with regard to skin irritation, photostability and inorganic powder.

Test Example 3

Skin Irritation Test

Sample (10 wt % of ultraviolet absorbent) is the same as test example 2.

(i ) Continuous use test

The continuous use test by the healthy subjects was carried out with one group of twenty subjects. A proper amount of each sample was applied to the face twice a day for 4 weeks. The evaluation standard of Table 5 was judged.

(Evaluation)

The calculated average score was evaluated by the next standard.

A: Average score is 0.

B: Average score is over 0 and less than 1.

C: Average score is 1 or more, and less than 2.

D: Average score is 2 or more.

The result was shown in Table 6.

(ii) Patch test

An occlusive patch test was carried out in the antebrachium part of healthy men and women subjects by finchamber for 24 hours. Each group was twenty subjects. The judgement standard is shown in Table 7.

(Evaluation)

Each of the calculated average scores was evaluated by the following evaluation standard.

A: average score is 0.

B: average score is over 0 and less than 1.

C: average score is 1 or more and less than 2.

D: average score is 2 or more.

The result was shown in Table 8.

Table 6 and Table 8 shows that external preparation for skin including ultraviolet absorbent of the present invention does not cause skin irritation in continuous use test and patch test, Also, it is understood that external preparation for skin of the present is very safe.

Test Example 4

Photostability Test

After an aqueous solution of the pyridazine derivative of the present invention was exposed to sunlight (Amount of solar radiation exposure 80 MJ/m 2 ) for two weeks, residual yield and change of appearance were checked. UV absorption spectrum (Solvent: water, concentration: 10 ppm, Light path: 1 cm) was measured by spectrophotometer. Area value was calculated by integrating over the range of 290 nm to 400 nm of the ultraviolet rays absorption spectrum. The area value was compared with the value before sunlight exposure.

(Evaluation standard)

The residual yield and change of area value of ultraviolet rays absorption spectrum were evaluated by the following standard.

A: 95% or more of area value before sunlight exposure.

B: 90% or more and less than 95% of area value before sunlight exposure.

C: 70% or more and less than 90% of area value before sunlight exposure.

D: less than 70% of area value before sunlight exposure.

The result was shown in Table 9.

Table 9 shows that a pyridazine derivative of the present invention has a very high residual yield. Accordingly, pyridazine derivative of the present invention did not decomposed by direct sunlight exposure for a long time. Also, the shape and area value of ultraviolet ray absorption spectrum did not change. Also, coloring and deposition and so on in the appearance were not found.

Test Example 6

Stability Test In Case Of Including UV Shielding Agent Of Inorganic Powder

The sun screen cream of the following formulation was manufactured. These were preserved for 2 months at 50° C. By visual observation of discoloration, the inventors have checked stability when using an UV shielding agent of inorganic powder which is included as external skin preparation for the ultraviolet rays defense.

(Manufacturing method)

After (2) was added to (1) and was swelled sufficiently, (3) to (11) was added thereto and was mixed with heating. The mixture was sufficiently dissolved with dispersing. This dispersed liquid was kept at 70° C. After this dispersed liquid was emulsified homogeneously by homomixer with adding a mixture of (12) to (15) gradually, which was cooled to 30° C. with stirring well to obtain sun screen.

The result was shown in Table 10.

Table 10 shows that discoloration is not found in a pyridazine derivative of the the present invention in the case where inorganic powder is used.

Accordingly, pyridazine derivatives of the present invention do not cause skin irritation and excel in photostability. Also, discoloration does not result in case of use of inorganic powder. Accordingly, pyridazine derivatives of the present invention are very useful as an ultraviolet absorbent in an external skin preparation.

Next, the effect as a photostabilizer of pyridazine derivative of the present invention was studied.

First of all, the photostabilization effect and appearance change of a composition in each pigment were studied by the following evaluation formulation.

Formulation for Evaluation of Colorant Stabilization Effect

Each test sample was prepared. Observation of appearance change (visual evaluation) and measurement of color difference (ΔE) were carried out in samples exposed to sunlight exposure (around 80 MJ).

Color difference was measured by Lab coordinate system with spectrophotometer. Color difference was calculated on the basis of the color before sunlight exposure. Namely, from measured value (L 1 ,a 1 ,b 1 ) before sunlight exposure, color difference (ΔE) was calculated by following formula.

ΔE={(L 2 −L 1 ) 2 +(a 2 −a 1 −a 1 ) 2 +(b 2 −b 1 ) 2 } ½

Table 11 and Table 12 show the result of the combination of a single colorant and various kinds of photostabilizer.

Next, Table 13 shows the result of the combination of multiple pigments and various kinds of photostabilizer.

Tables 11 to 13 show that the color difference ΔE in a pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention is very small in comparison with other photostabilizers. Also, the change of appearance of the composition is small. Accordingly, it is understood that pyridazine derivative of the present invention has excellent photo stability for colorant.

Next, the inventors studied the effective amount of photostabilizer of the present invention for pigment. Table 14 and Table 15 show the result of combination of a pyridazine derivative of the present invention and a single colorant.

Table 16 shows the result of combining a pyridazine derivative of the present invention and multiple colorant.

Tables 14 to 16 show that approximately 0.01 wt % to approximately 0.3 wt % of pyridazine derivatives of the present invention is effective as a photostabilizer in approximately 0.00001 wt % to approximately 0.001 wt % of colorant. Also, although over 0.3 wt % of pyridazine derivative is possible, in case of external preparation for skin, if the amount is greater than 20 wt % of pyridazine derivative, it is difficult to maintain the formulation of the external skin preparation.

Next, the photostabilization effect for each perfume was studied by the following evaluation formulation.

Formulation For Evaluation Of Perfume Stabilization Effect

Each test sample was prepared. Change of smell of sample exposed to sunlight (80 MJ) was observed (judgement by perfumier).

Table 17 shows the result of combining of natural perfume and various photostabilizers.

Table 17 shows that the change of smell in a pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention is very small in comparison with other photostabilizers. Accordingly, it is understood that pyridazine derivative of the present invention has an excellent photostabilization effect for natural purfume.

Table 18 shows the result of combining synthetic perfume and various photostabilizers.

Table 18 shows that the change of smell in a pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention is very small in comparison with other photostabilizers. Accordingly, it is understood that pyridazine derivatives of the present invention have an excellent photostabilization effect for synthetic purfume.

Table 19 shows the result of combining base perfume and various photostabilizers.

Table 19 shows that the change of smell in a pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention is very small in comparison with other photostabilizers. Accordingly, it is understood that pyridazine derivatives of the present invention have an excellent photo stabilization effect for base perfume.

Next, the inventors have studied the effective amount of photostabilizer for perfume. Table 20 shows the result of combining a pyridazine derivative of the present invention and natural perfume.

Table 20 shows that approximately 0.001 wt % to approximately 0.3 wt % of a pyridazine derivative of the present invention is effective as a photostabilizer in approximately 0.03 wt % of natural perfume.

Next, Table 21 shows the result of combining a pyridazine derivative of the present invention and synthetic perfume.

Table 21 shows that approximately 0.01 wt % to approximately 0.3 wt % of a pyridazine derivative of the present invention is effective as a photostabilizer for approximately 0.03 wt % of synthetic perfume.

Next, Table 22 shows the result of combining a pyridazine derivative of the present invention and a base perfume.

Table 22 shows that approximately 0.01 wt % to approximately 0.3 wt % of a pyridazine derivative of the present invention is effective as a photostabilizer for approximately 0.03 wt % of base perfume.

Next, the photo stabilization effect and changes in appearance in drug compositions were studied according to the following evaluation formulation.

Formulation For Evaluation Of Drug Stabilization Effect

Each test sample was prepared. Appearance changes of the samples exposed to sunlight (80 MJ) was observed (visual evaluation). Also, residual yield was measured by liquid chromatography.

Next, Table 23 shows the result of combining a pyridazine derivative of the present invention and a drug.

Table 23 shows that residual yield of a drug combined with a pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention is very high in comparison with other photostabilizers. Also, appearance changes of the composition is small. Accordingly, it is understood that pyridazine derivatives of the present invention have an excellent photostabilization effect on drugs.

The inventors have attempted to improve the photostabilzation effect by combining the composition with sequestering agent.

First of all, the photostabilization effect and appearance changes of a composition for each pigment were studied by the following evaluation formulation.

Formulation For Evaluation Of Pigment Stabilization Effect

(Sequestering agent combination)

Each test sample was prepared. Observation of appearance changes (visual evaluation) and measurement of color difference (ΔE) were carried out for samples exposed to sunlight (around 80 MJ).

Color difference was measured by Lab coordinate system with spectrophotometer. Color difference was calculated based on the color before sunlight exposure. Namely, from the measured value (L 1 ,a 1 ,b 1 ) before sunlight exposure, color difference (ΔE) was calculated by following formula.

ΔE={(L 2 −L 1 ) 2 +(a 2 −a 1 ) 2 +(b 2 −b 1 ) 2 } ½

Table 24 and Table 25 shows the result of combining a single pigment, a pyridazine derivative of the present invention and various sequestering agents.

Next, Table 26 is the results in compositions having various pigments, a pyridazine derivative of the present invention and various kinds of sequestering agents.

Tables 24 to 26 show that color difference ΔE for compositions having pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention and a sequestering agent is very small in comparison with color difference ΔE for other compositions not having a sequestering agent. Also, the change of appearance of the composition is small. Accordingly, it is understood that pyridazine derivatives of the present invention have a better photostabilization effect for pigment when combined with a sequestering agent.

Also, since a sequestering agent itself does not have a photostabilization effect, combining a pyridazine derivative of the present invention and a sequestering agent has synergistic photostabilization effect.

Next, for combinations with sequestering agent, photostabilization effect for each perfume was studied by the following evaluation formulation.

Formulation For Evaluation Of Perfume Stabilization Effect

(Sequestering agent combination)

Each test sample was prepared. Smell change of samples exposed to sunlight (80 MJ) was observed (judgement by perfumier).

Table 27 shows the result of combining natural perfume, a pyridazine derivative of the present invention and various sequestering agents.

Table 27 shows that smell change of a composition including a pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention and a sequestering agent is very small in comparison with smell change of other compositions not having sequestering agent. Accordingly, it is understood that a pyridazine derivative of the present invention has a better photostabilization effect for natural perfume by combining it with a sequestering agent.

Also, since the sequestering agent itself does not have a photostabilization effect, combining a pyridazine derivative of the present invention and a sequestering agent has a synergistic photostabilization effect.

Table 28 shows the result of combining a synthetic perfume, a pyridazine derivative of the present invention and various sequestering agents.

Table 28 shows that smell change of a composition including a pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention and a sequestering agent is very small in comparison with smell change of other compositions not having a sequestering agent. Accordingly, it is understood that a pyridazine derivative of the present invention has a better photostabilization effect for synthetic perfume by combining it with a sequestering agent.

Also, since the sequestering agent itself does not have a photostabilization effect, combining of a pyridazine derivative of the present invention and a sequestering agent has a synergistic photostabilization effect.

Table 29 shows the result of combining a base perfume, a pyridazine derivative of the present invention and various sequestering agents.

Table 29 shows that smell change of a composition including a pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention and a sequestering agent is very small in comparison with smell change of other compositions not having a sequestering agent. Accordingly, it is understood that a pyridazine derivative of the present invention has a better photostabilization effect for base perfume by combining it with a sequestering agent.

Also, since the sequestering agent itself does not have a photostabilization effect, combining a pyridazine derivative of the present invention and a sequestering agent has a synergistic photostabilization effect.

Next, when combined with a sequestering agent, the photostabilization effect and appearance change of a composition for each drug was studied by the following evaluation formulation.

Formulation For Evaluation Of Drug Stabilization Effect

(Sequestering agent combination)

Each test sample was prepared. Appearance change of the samples exposed to sunlight (around 80 MJ) was observed (visual evaluation). Also, residual yield of a drug was measured by liquid chromatography.

Next, Table 30 shows the result of combining a drug, a pyridazine derivative of the present invention and various sequestering agents.

Table 30 shows that residual yield of a drug in a composition having a pyridazine derivative (4,5-dimorpholino-3-hydroxypyridazine) of the present invention and a sequestering agent is very small in comparison with residual yield of a drug in other compositions not having a sequestering agent. Accordingly, it is understood that pyridazine derivative of the present invention has a better photostabilization effect for a drug when combined with a sequestering agent.

Also, since a sequestering agent itself does not have a photostabilization effect, combining of pyridazine derivative of the present invention and a sequestering agent has a synergistic photostabilization effect.

The following are examples of external skin preparations of the present invention. These examples do not limit the present invention. Amounts shown are weight percent.

Each of water phase and alcohol phase was prepared and further mixed.

A portion of the ion-exchanged water, methyl cellulose and quince seed were mixed with stirring and a viscous liquid was prepared. The rest of the ion-exchanged water and other water phase ingredients were mixed with dissolving. The above-mentioned viscous liquid was added to this and a homogeneous water phase was obtained. The prepared alcohol phase was added to the water phase and was mixed.

Propylene glycol and potassium hydroxide were added to ion-exchanged water and were dissolved. The mixture was heated and was kept at 70° C. (Water phase). A mixture of the other components was melted with heating and was kept at 70° C. (Oil phase). The oil phase was gradually added to the water phase and an emulsion was formed. After it was homogeneously emulsified with a homomixer, which was cooled to 30° C. with sufficient stirring.

The propylene glycol and 4,5-dimorpholino-3-hydroxypyridazine were added to ion-exchanged water and were dissolved. It was kept at 70° C. with heating (Water phase). A mixture of the other ingredients was melted with heating and was kept at 70° C. (Oil phase). The oil phase was added gradually to the water phase and an emulsion was formed. After it was emulsified homogeneously with a homomixer, it was cooled to 30° C. with sufficient stirring.

Carboxyvinylpolymer was dissolved in a small amount of ion-exchanged water (A phase). Polyethylene glycol 1500, 4,5-dimorpholino-3-hydroxypyridazine hydrochloaide and triethanolamine were added to the remainder of the ion-exchanged water, which was dissolved with heating and was kept at 70° C. (Water phase). Mixture of other ingredients was melted with heating and was kept at 70° C. (Oil phase). The oil phase was added to the water phase to form an emulsion was formed. After A phase was added and was homogeneously emulsified with a homomixer, it was cooled to 30° C. with sufficient stirring.

Carboxyvinylpolymer was dissolved in ion-exchanged water homogeneously (A phase). 4,5-Dimorpholino-3-hydroxypyridazine and POE (50) oleyl ether were dissolved in 95% ethanol, which was added to A phase. After the ingredients other than sodium hydroxide were added, sodium hydroxide was added thereto, thereby neutralizing the composition and increasing viscosity.

Each of (A phase) and (C phase) was homogeneously dissolved. (C phase) and additive (A phase) were solubilized. Next, (B phase) was added and mixed.

Each of A phase, (B phase) and (C phase) was homogeneously dissolved. (A phase) was added to (B phase) and was solubilized. Next, (C phase) was added and mixed.

The above-mentioned examples 1 to 7 had an excellent ultraviolet rays prevention effect. Also, in examples 1 to 8, skin trouble was not observed at all.

Each of oil phase and water phase was dissolved at 70° C. Oil phase was mixed with water phase and was emulsified with emulsifier. Next, the result was cooled to 30° C. with a heat exchanger.

The milky lotion of example 9 had an excellent ultraviolet rays prevention effect. Also the skin trouble was not observed.

Each of (1) to (8) was mixed with crushing. A mixture of components of (9) to (14) were added thereto and was mixed with agitation. Solid foundation was obtained by forming to the container.

Ingredients (9) to (15) were mixed and were homogeneously dissolved. A crushed (1) to (7) were added thereto and dispersed. (8) was added to this dispersion liquid and was emulsified. A W/O emulsion foundation was obtained by forming to container.

Each ingredient of (1) to (6) was mixed and crushed. Mixture of each ingredient of (7) to (12) was added and mixed with agitation and a face powder was obtained.

Components of (1) to (6) were crushed and mixed. Furthermore, a mixture of the components of (7) to (12) was added thereto, which was mixed with agitation and an eye shadow was obtained.

Ingredient (9) and (10) were dispersed to (8) melted at 60° C. (11) was added to this and was stirred sufficiently. This was added to heated and dissolved (1) to (7) and was agitated sufficiently. After (12) to (14) was added thereto which was dispersed with stirring, lipstick was obtained by molding.

Makeup cosmetics of examples 10 to 14 have an excellent ultraviolet ray prevention effect. No skin trouble or no discoloration was observed.

Liquid petrolatum was added to dissolved glycerol and polyoxyethylene hydrogenated castor oil and was homogeneously emulsified with a homomixer. This was added to solution of the other ingredients. After the undiluted solution was filled a can, the valve was fixed and gas was added.

Polyoxypropylene (40) butyl ether, polyoxyethylene hydrogenated castor oil, 4,5-dimorpholino-3-hydroxypyridadine, perfume and antiseptics were dissolved in ethanol. Colorant was dissolved in ion-exchanged water. Water phase was added to Ethanol phase and was filtered with filter paper.

Other ingredients were added to ethanol and dissolved and the result was filtered. After undiluted solution was added to a can and the valve was fixed, gas was added.

The hydrogenated castor oil, ethylene oxide (40 moles) additives and 4,5-dimorpholino-3-hydroxypyridazine were dissolved in ethanol. The ethanol phase and water phase were mixed and perfume was added.

The cosmetics for hair and scalp of examples 15 to 18 had an excellent ultraviolet ray prevention effect. Also, scalp trouble and discoloration over of time were not observed.

The water phase and alcohol phase that were prepared individually were mixed.

Propylene glycol, L-ascorbic acid 2-(dl-α-tocopheryl hydrogen phosphate) potassium salt, 4,5-dimorpholino-3-hydroxypyridazine and potassium hydroxide were added to ion-exchanged water and were dissolved. It was kept with heating at 70° C. (Water phase). Other ingredients were melted with heating and kept at 70° C. (Oil phase). The oil phase was added gradually to the water phase and was emulsified preliminarily. After oil phase was added to water phase and was emulsified homogeneously with a homomixer, it was cooled to 30° C. with sufficient stirring.

Carboxyvinylpolymer was dissolved in a small amount of ion-exchanged water (A phase). Polyethylene glycol 1500, L-ascorbic acid 2-(dl-α-tocopheryl hydrogen phosphate) potassium salt, 4,5-dimorpholino-3-hydroxypyridazine and triethanolamine were added to the remainder of the ion-exchanged water. It was dissolved with heating and was kept at 70° C. (Water phase). A mixture of other ingredients was melted with heating and was kept at 70° C. (Oil phase). The oil phase was added to the water phase and was emulsified preliminarily. After A phase was added thereto and was emulsified homogeneously with a homomixer, which was cooled to 30° C. with sufficient stirring.

Pigment was added to a part of acetyltributyl citrate and a part of alkyd resin and was kneaded well (Pigment part) Other ingredients were mixed and dissolved. The pigment part was added to this, stirred well, and homogeneously dispersed.

Each component was added to a purified water at 70° C. The mixture was homogeneously dissolved and cooled.

Stearyltrimethyl ammonium chloride, glycerol and pigment were added to a purified water and was kept at 70° C. (Water phase). Mixed other ingredients were dissolved with heating and was kept at 70° C. (Oil phase). The oil phase was added to the water phase. The mixture was emulsified with a homomixer, which was cooled with stirring.

Pyridazine derivatives and salts thereof of the present invention, as an ultraviolet absorbent absorbs strongly ultraviolet rays of all wavelengths with the range of 290 nm to 400 nm which reach surface of the earth. Accordingly, this absorbent has excellent ultraviolet ray absorption ability. Also, thereof it has high safety and high stability. Also, pyridazine derivatives and salts of the present invention demonstrate an excellent effect as a photostabilizer of colorant, perfume and drug. Especially, by combining a sequestering agent, this effect can be synergistically enhanced. Accordingly, by combining the pyridazine derivative of the present invention, the obtained external preparation for the skin has high ultraviolet rays prevention effect, good stability, good safety and good photostability.

Another use other than for the external skin preparations is an ultraviolet ray absorption composition which has excellent ultraviolet ray prevention effect.