The present invention relates to a novel chiral compound of Hibiscus acid bearing lactone moiety of formula I, Wherein:R1R2alkali salt of carboxylic acid or acid chloride or lower esters or the N-substituted cyclic imides.R3hydroxyl or protected hydroxyl group and a process for preparing the same.

Description

13 parts

›FIELD OF THE INVENTION

The invention relates to novel chiral derivatives of Hibiscus acid bearing lactone ring moiety and the process for preparing the same.

›BACKGROUND OF THE INVENTION · 1 of 2

Hibiscus acid [(+)-Hydroxycitric acid lactone or (2S,3R)-Tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylic acid] can be isolated from the leaves/fruit calyxes of Hibiscus sabdariffa or from the leaves of Hibiscus furcatus , and Hibiscus cannabinus . Garcinia acid [(−)-Hydroxycitric acid lactone or (2S,3S)-Tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylic acid,], a diastereomer of Hibiscus acid is widely used as an important ingredient in many pharmaceutical formulations 1-10.

The non-availability of Hibiscus acid in the market, in the optically pure form, has resulted in the limited use of Hibiscus acid or its derivatives in the broad area of organic synthesis and pharmaceutical front. This is due to the lack of any commercially viable large-scale manufacturing process. In U.S. patent application Ser. No. 09/365,300 an economic, commercially viable, cost effective process for the large-scale isolation of Hibiscus acid has been described 11 .

Also during the past two decades there has been a great deal of interest to find cheap and potential chiral molecules from chiral pool to accomplish synthetic pathways with a high degree of asymmetric induction 12-25.

Added to this, substituted γ-butyrolactones are known to be potent antagonists or agonists depending upon the substitution pattern of the γ-aminobutyric acid receptor, the major inhibitory neurotransmitter in the mammalian central nervous system 26 .

The known methods for obtaining diversity functionalised chiral γ-lactones are either by the cyclisation of acyclic starting materials such as the sterioselective iodolactonisation of unsaturated 3-hydroxy acids 27 or from sugars such as D-ribofuranose or D-glucosamine or carbohydrates such as D-ribose, D-glucose etc 28 . These chemical modifications involving carbohydrates require tedious protocols.

Existing Methods:

a. The method reported by Per. M. Boll, Else Sorensen and Eric Balieu 29 for the isolation of Hibiscus acid is from the calyxes of the fruits of Hibiscus sabdariffa . In this method dried, ground fruit calyxes of Hibiscus sabdariffia is extracted at room temperature for 68 hours several times with methanol containing 1.5% hydrogen chloride. To the pooled methanol extracts, ether is added and the coloring matter is deposited as a dark red syrupy mass. The ether layer is collected and syrup is dissolved in methanolic hydrogen chloride (1%) and again precipitated by the addition of ether. The pooled ether extracts is evaporated and the residue is dissolved in methanol. Upon cooling colorless crystals is obtained and the same is recrystallised from propanol and which was later hydrolysed to get Hibiscus acid.

b. Another method for the laboratory-scale production of Hibiscus acid described by C. Martius and R. Maue 30 , is purely a synthetic one. In this method Hibiscus acid is prepared from a number of chemical constituents and not from any natural source.

c. The method described by this author and co-workers is a general method for the isolation of Hibiscus acid in the optically pure crystalline form from the fresh or dried leaves and/or calyxes of Hibiscus sabdariffa and leaves of Hibiscus furcatus or Hibiscus cannabinus employ mostly organic solvents. The main extraction is done with acidic methanol, followed out by the removal of organic impurities by adding water. The resulting filtrate was further extracted with organic solvents to get crude Hibiscus acid, which was later purified by esterification followed by hydrolysis.

The Drawbacks of the Existing Method “a” are:

1. The method fails to get pure Ia when leaves of the plants is used and is applicable only in the case of the fruit calyxes of Hibiscus sabdariffa.

2 . Hibiscus sabdariffa is a seasonal flowering plant and hence the calyxes is not available at any given time.

3. Large quantities of expensive solvent ether is required for the process.

4. Crystallisation was effected only on prolonged (2 months) storage over drierite, in a desiccater.

Method “b” describes the synthesis of formula Ia from chemical constituents and is not economically viable.

None of the existing methods (a&b) describes the complete characterization and degree of purity of the compound.

Method “c” describes basically the extraction with undesirable methanol and expensive diethyl ether as solvents.

The object of the present invention therefore is to prepare chial derivatives bearing lactone ring moiety and to provide a new method obviating the drawbacks of the existing methods.

To achieve the objectives, this invention provides novel chiral derivatives of Hibiscus acid bearing lactone ring moiety of formula I

Wherein:

R 1 ═R 2 ═alkali salt of carboxylic acid or acid chloride or lower esters or the N-substituted cyclic imides.

R 3 ═hydroxyl or protected hydroxyl group

In the above formula I

R 1 and R 2 is selected from

—COONa, —COCI, —COOCH 2 C 6 H 5 , —COOC 2 H 5 ,

R 2 is —OH or protected hydroxyl group to form various chiral derivatives of Hibiscus acid bearing lactone ring moiety.

Chiral Derivatives of Hibiscus Acid Bearing Lactone Ring Moiety:

Summary of the Chiral Derivatives of Hibiscus Acid Bearing Lactone Ring Moiety is Given Below in Scheme I:

Compound of formula Ib is Disodium (2S,3R)-tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylate

Compound of formula Ic is (2S,3R)-Tetrahydro-3-hydroxy-5-oxo-2,3-furandicarbonylchloride

Compound of formula Id is Dimethyl (2S,3R)-tetrahydro-3-oxo-[(methylthio)methoxy]-5-oxo-2,3-furandicarboxylate

Compound of formula Ie is (phenylmethyl)(2S,3R)-tetrahydro-3-hydroxy-5-oxo-2,3 furandicarboxylate

Compound of formula Ig is Diethyl (2S,3R)-tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylate.

Compound of formula Ih is s Diisopropyl (2S,3R)-tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylate.

Compound of formula Ij is (3aR,6aS)-3a-(acetyloxy)dihydro-5-(4-methoxy-phenyl methyl)-6H-furo[2,3-c]pyrrole-2,4,6(3H,4H)-trione

The present invention further provides a process of isolation of compound of Formula Ia, comprising:

extracting the Calyxes/leaves of Hibiscus sabdariffa , leaves of Hibiscus furcatus and Hibiscus cannabinus using water (X),

›BACKGROUND OF THE INVENTION · 2 of 2

washing the extract (X) with organic solvent to remove impurities,

concentrating the aqueous layer,

adding an organic solvent to remove insoluble impurities,

evaporating the organic layer,

adding aqueous alkali to the concentrate to yield the alkali salt,

purifying the alkali salt by the addition of alcohol,

readjusting the pH by the addition of mineral acid,

concentrating and extracting the solution with organic solvents,

further concentrating the solution to get a syrup,

extracting the said syrup with solvents,

concentrating the obtained solution to get pure Ia in crystalline form.

The organic solvent used for washing is hexane.

The organic solvent used for removing impurities is selected from methanol or acetone.

The invention further includes a process for preparing the chiral derivative of Hibiscus acid of formula Ib comprising:

treating the aqueous solution of Ia with aqueous solution of alkali till the pH of the solution is neutral,

evaporating the resultant solution to dryness,

washing the residue with water miscible organic solvent,

drying the product Ib under vacuum.

The said alkali is sodium bicarbonate.

The invention further includes a process for preparing the chiral derivative of formula Ic comprising:

adding an organic halide to a suspension of Ib in organic solvent,

stirring the mixture for 1-4 hours, filtering the said mixture,

evaporating the said solution to get Ic as a hygroscopic solid.

The said organic solvent is ether.

The said organic halide is thionyl chloride.

The invention also includes a process for preparing the chiral derivative of Formula Id comprising:

adding DMSO, an organic acid and an anhydride to If,

allowing the mixture to stand for 34 days,

adding the reaction mixture to cold saturated aqueous solution of alkali,

stirring the mixture for 14 hours,

extracting the resultant solution with an organic solvent,

washing the extract with aqueous alkali,

drying the organic layer,

evaporating to get crude Id,

purifying the crude Id by chromatography to get pure Id as an yellow oil.

The said organic acid is acetic acid.

The said anhydride is acetic anhydride.

The said alkali is sodium bicarbonate.

The said organic solvent used for extraction is chloroform.

The invention further includes a process for preparing the chiral derivative of formula Ie wherein, comprising:

refluxing Ia with an appropriate alcohol and organic acid in toluene for 10-20 hours using Dean-Stark set up,

washing the mixture with aqueous alkali solution,

evaporating the organic phase,

recrystallising from organic solvents or their appropriate mixtures yielding Ie as a solid.

The said appropriate alcohol is benzyl alcohol.

The said anhydride is acetic anhydride.

The said alkali is sodium bicarbonate.

The said organic solvent used for crystallization is selected from hexane or ether.

The invention further includes a process for preparing the chiral derivative of formula Ig comprising:

adding organic halide to a suspension of Ib in absolute alcohol,

stirring the mixture for 24 hours,

neutralizing the mixture with aqueous alkali solution,

extracting the said mixture using organic solvent,

evaporating the mixture furnishing Ig as a yellow oil.

The said organic halide is thionyl chloride.

The said alcohol is ethanol.

The said alkali is sodium bicarbonate.

The said organic solvent is chloroform.

The invention further includes a process for preparing the chiral derivative of formula Ih comprising:

adding an organic halide to a suspension of Ib in appropriate dry alcohol,

stirring the mixture for 36 hours,

neutralizing with aqueous alkali solution,

extracting the said solution with an organic solvent.

evaporating and extracting using an appropriate organic solvent yielding Ih as a yellow oil.

The said organic halide is thionyl chloride.

The said appropriate alcohol is isopropyl alcohol.

The said appropriate alkali is sodium bicarbonate.

The said appropriate organic solvent is chloroform.

The organic solvent used after evaporation is hexane.

The invention further includes a process for preparing the chiral derivative of formulae Ij or Ik comprising:

refluxing the suspension of Ia in an organic halide for 3 hours,

concentrating the said mixture under vacuum,

dissolving the solid obtained in an organic solvent,

adding an appropriate amine to the dissolved solution,

stirring the mixture at room temperature for 4-18 hours

concentrating the solution under vacuum,

adding the organic halide to the semi-solid obtained

refluxing for 18 hours,

extracting with suitable organic solvent

subjecting the said extract to chromatography furnishing Ij or Ik as white crystals.

The said organic halide is acetyl chloride.

The said appropriate amine is 4-methoxy benzyl amine.

The said organic solvent is chloroform.

The process will now be described with reference to the foregoing examples.

›Examples8

›EXAMPLE 1

Hibiscus acid (Ia)

Ia is isolated from the leaves of Hibiscus furcatus/Hibiscus subdariffa or from the calyxes of Hibiscus sabdariffa as given below (A and B).

A. Fresh leaves (1 kg) of Hibiscus furcatus was extracted with water (1 Lt). The concentrated aqueous extract was washed with hexane several times. To the aqueous layer, acetone (1 Lt) was added to precipitate insoluble materials. After evaporating the acetone, NaOH solution (8N, 80 ml) was added to adjust the pH to 12.0. Addition of alcohol (1 Lt) resulted in the precipitation of Na salt of Ia. The pH was re-adjusted to 2.0 by the addition of HCl (2N). The combined mixture was concentrated to a syrupy mass and extracted with dry acetone (2×250 ml). The acetone extract was concentrated. This was further extracted with ether (3×150 ml), followed by concentration yielded Ia (10 g) in the pure form.

B. Fresh calyxes (1 kg) of Hibiscus sabdariffa was extracted following the procedure described above (example IA) yielded Ia (16 g) in the pure form.

Melting point: 175° C. (decomp.)

›EXAMPLE 2

Disodium (2S,3R)-tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylate (Ib):

To an aqueous solution of Ia (1.0 g, 5 mmol, in 5 ml water), saturated sodium bicarbonate solution was added till the pH of the solution is neutral. The residue obtained after evaporation was washed with dry acetone (5×10 ml). The product Ib was finally dried under vacuum.

Yield: 1.0 g (82%).

›EXAMPLE 3

(2S,3R)-Tetrahydro-3-hydroxy-5-oxo-2,3-furandicarbonylchloride (Ic)

To a suspension of Ib (1.0 g, 4.3 mmol) in ether (10 ml), thionyl chloride (1.0 ml, 14 mmol) was added. The mixture was stirred for two hours. The reaction mixture was filtered followed by evaporation gave Ic.

Yield: 0.6 g (62%).

›EXAMPLE 4

Dimethyl (2S,3R)-tetrahydro-3-oxo-[(methylthio)methoxy]-5-oxo-2,3-furandicarboxylate (Id):

To a solution of If (5 g, 22.9 mmol) in DMSO (70 ml), acetic acid (6 ml) in acetic anhydride (50 ml) was added. The mixture was allowed to stand for three days. The reaction mixture was added to saturated aqueous solution of sodium bicarbonate (900 ml) and stirred for one hour. It was extracted with chloroform (3×350 ml) and the combined chloroform extracts was washed with saturated sodium bicarbonate solution (100 ml) followed by water (2×50 ml). The chloroform extract was dried (sodium sulphate) and evaporated to get crude Id (4 g). Id was further purified by column chromatography (silicagel 60-120 mesh, eluent: hexane-chloroform, 10-50%.

Yield: 2.5 g (38%)

›EXAMPLE 5

Bis(phenylmethyl)(2S,3R)-tetrahydro-3-hydroxy-5-oxo-2,3 furandicarboxylate (Ie)

Ia (3.8 g, 20 mmol) was refluxed with benzyl alcohol (6.5 g, 60 mmol) and p-toluene sulphonic acid (50 mg, 0.25 mmol) in toluene (40 ml) for 13 hours using a Dean-Stark set up. The mixture was washed with aqueous sodium bicarbonate solution (50 ml). The aqueous phase was extracted using chloroform (20 ml). Evaporation of the combined extracts yielded Ie which was recrystallised from hexane-ether.

Melting point: 90° C. Yield: 3 g (4%)

›EXAMPLE 6

Diethyl (2S,3R)-tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylate (Ig):

To a precooled (−5-0° C.) suspension of Ib (1.0 g, 4.4 mmol) in dry ethanol (10 ml), thionyl chloride (0.7 ml, 10 mmol) was added. The mixture was then stirred for 24 hours at room temperature. After filtration of the reaction mixture, pH of the filtrate was adjusted to 7.0, by adding saturated aqueous solution of sodium bicarbonate and was extracted with chloroform (3×10 ml). The combined extract upon drying and evaporation furnished an oily residue of Ig.

Yield: 0.9 g (7%)

›EXAMPLE 7

Diisopropyl (2S,3R)-tetrahydro-3-hydroxy-5-oxo-2,3-furandicarboxylate (Ih):

To a precooled (−5-0° C.) suspension of Ib (1.0 g, 4.4 mmol) in isopropyl alcohol (10 ml) and thionyl chloride (0.7 ml, 10 mmol) was added. The mixture was then stirred for 36 hours at room temperature. After filtration of the reaction mixture, pH of the filtrate was adjusted to 7.0, by adding saturated aqueous solution of sodium bicarbonate and was extracted with chloroform (3×10 ml). The organic layer was concentrated and extracted using hexane. The combined extract upon drying and evaporation furnished an oily residue of Ih.

Yield: 0.1 g (41%)

›EXAMPLE 8

(3aR,6aS)-3a-(acetyloxy)dihydro-5-(4-methoxy-phenyl methyl)-6H-furo[2,3-c]pyrrole-2,4,6(3H,4H)-trione(Ij)

A suspension of Ia (1 g, 5 mmol) in acetyl chloride (4 ml) is refluxed for 3 hours followed by concentration under vacuum. The solid obtained is dissolved in THF (5 ml) and 4-methoxy benzyl amine (0.6 ml, 5 mmlol) is added. The mixture is stirred at room temparature for 18 hours followed by concentration under vacuum. To the semi-solid obtained acetyl chloride (5 ml) is added and the mixture is refluxed for 18 hours. Concentration under vacuum followed by column chromatography afforded Ij as white crystals. (Active neutral alumina, eluent: chloroform-hexane 50%)

Yield: 0.2 g (12%)

Advantages of the New Process:

1. Basically, the extraction is done with water. The use of undesirable solvent Methanol is totally replaced by water and use of solvent ether is reduced considerably. (Following the present method, for the isolation of 100 gms of hibiscus acid four liters of diethyl ether is used. However following the prior art fifty liters of ether is used in the second step for the isolation of 100 gms of the acid.)

2. The present method is general for fresh/dried leaves and calyxes of Hibiscus sabdariffa , leaves of Hibiscus furcatus and Hibiscus cannabinus . Process time is substantially reduced and the method is inexpencive. The leaves of the above plants are available through out South India in all seasons, in plenty.

3. The process is suitable for large-scale isolation and involves simple crystallisation techniques.

4. This process is a water process and which is entirely new from the method described in any of the prior arts.

›USES

Pharmaceutical applications

Chiral derivatives, Ia-Ik, are used as chiral synthons

›REFERENCES

1. U.S. Pat. No. 4,005,086 dated Jan. 1, 1997

2. U.S. Pat. No. 4,006,166 dated Feb. 1, 1977

3. U.S. Pat. No. 4,007,208 dated Feb. 8, 1997

4. U.S. Pat. No. 5,536,516 dated Jul. 7, 1996

5. U.S. Pat. No. WO 9605741 Al 960229

6. U.S. Pat. No. WO 9636585 Al 961121

7. CA 86, 1977. 186629r.

8. CA 85, 1976, 41531x.

9. CA 87, 1977, 195626k.

10. CA 96, 1982, 30421n.

11. U.S. patent application Ser. No 09/365,300, 1999

12. Tetrahedron Letters Vol. 25 pp 4491-4494, 1984

13. Tetrahedron Vol. 43, No. 19, pp 4497-4506, 1987

14. Tetrahedron Vol. 38, No. 15, pp 2377-2394, 1982

15. Tetrahedron Vol. 34, pp 1449-1452, 1978

16. J. Org. Chem. 63, 2385-2388, 1998

17. J C S Chem. Comm pp 711, 1973

18. J. Org. Chem. 58, 2725-2737, 1993

19. Tetrahedron Vol. 31, pp 3011-3012, 1975

20. Tetrahedron Letters Vol. 22, No. 52, pp 5271-5274, 1981

21. Tetrahedron Letters Vol. 23, No. 48, pp 5051-5054, 1982

22. J. Org Chem. 1984, 49, 5041

23. Synthesis 89, 1986

24. J. Org. Chem. 1997, 62, 8560

25. Tetrahedron, Vol. 52. No. 7, pp 2603-2628, 1996

26. J. Org. Chem. 1993, 58, 2725

27. Tetrahedron Letters 1981, 22, 4611

28. J. Org Chem. 1984, 49, 2168 and references sited there in

29. Acta. Chem. Scand. 23, pp 286-293, 1969

30. Z. Physiol. Chem. 33, 1941, 269

›Tables in the description — 1

Ib-	R 1 = R 2 = —COONa,	R 3 = —OH
Ic-	R 1 = R 2 = —COCl,	R 3 = —OH
Id-	R 1 = R 2 = —COOCH 3 ,	R 3 = —OCH 2 SCH 3
Ie-	R 1 = R 2 = —COOCH 2 C 6 H 5 ,	R 3 = —OH
Ig-	R 1 = R 2 = —COOC 2 H 5 ,	R 3 = —OH
Ih-	R 1 = R 2 = —COOCH(CH 3 ) 2 ,	R 3 = —OH
Ij-
R 3 = —OCOCH 3
Ik-
R 3 = —OCOCH 3

Claims as granted

33 claims

Classifications

4 codes

IPC · International Patent Classification

Section C — Chemistry; metallurgy

C07D491/04
C07D307/33

USPC · US Patent Classification

549/295549/313

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