Isolated human phosphatase proteins, nucleic acid molecules encoding human phosphatase proteins, and uses thereof

The present invention is in the field of phosphatase proteins that are related to the protein tyrosine phosphatase subfamily, recombinant DNA molecules and protein production. The present invention specifically provides novel phosphatase peptides and proteins and nucleic acid molecules encoding such peptide and protein molecules, all of which are useful in the development of human therapeutics and diagnostic compositions and methods.

Phosphatase proteins, particularly members of the protein tyrosine phosphatase subfamily, are a major target for drug action and development. Accordingly, it is valuable to the field of pharmaceutical development to identify and characterize previously unknown members of this subfamily of phosphatase proteins. The present invention advances the state of the art by providing a previously unidentified human phosphatase proteins that have homology to members of the protein tyrosine phosphatase subfamily.

Protein Phosphatase

Cellular signal transduction is a fundamental mechanism whereby external stimuli that regulate diverse cellular processes are relayed to the interior of cells. The biochemical pathways through which signals are transmitted within cells comprise a circuitry of directly or functionally connected interactive proteins. One of the key biochemical mechanisms of signal transduction involves the reversible phosphorylation of certain residues on proteins. The phosphorylation state of a protein may affect its conformation and/or enzymic activity as well as its cellular location. The phosphorylation state of a protein is modified through the reciprocal actions of protein phosphatases (PKs) and protein phosphatases (PPs) at various specific amino acid residues.

Protein phosphorylation is the ubiquitous strategy used to control the activities of eukaryotic cells. It is estimated that 10% of the proteins active in a typical mammalian cell are phosphorylated. The high-energy phosphate that confers activation and is transferred from adenosine triphosphate molecules to protein-by-protein phosphatases is subsequently removed from the protein-by-protein phosphatases. In this way, the phosphatases control most cellular signaling events that regulate cell growth and differentiation, cell-to-cell contacts, the cell cycle, and oncogenesis.

The protein phosphorylation/dephosphorylation cycle is one of the major regulatory mechanisms employed by eukaryotic cells to control cellular activities. It is estimated that more than 10% of the active proteins in a typical mammalian cell are phosphorylated. During protein phosphorylation/dephosphorylation, phosphate groups are transferred from adenosine triphosphate molecules to protein-by-protein phosphatases and are removed from the protein-by-protein phosphatases.

Protein phosphatases function in cellular signaling events that regulate cell growth and differentiation, cell-to-cell contacts, the cell cycle, and oncogenesis. Three protein phosphatase families have been identified as evolutionarily distinct. These include the serine/threonine phosphatases, the protein tyrosine phosphatases, and the acid/alkaline phosphatases (Carbonneau H. and Tonks N. K. (1992) Annu. Rev. Cell Biol. 8:463-93).

The serine/threonine phosphatases are either cytosolic or associated with a receptor. On the basis of their sensitivity to two thermostable proteins, inhibitors 1 and 2, and their divalent cation requirements, the serine/threonine phosphatases can be separated into four distinct groups, PP-I, PP-IIA, PP-IIB, and PP-IIC.

PP-I dephosphorylates many of the proteins phosphorylated by cylic AMP-dependent protein phosphatase and is therefore an important regulator of many cyclic AMP mediated, hormone responses in cells. PP-IIA has broad specificity for control of cell cycle, growth and proliferation, and DNA replication and is the main phosphatase responsible for reversing the phosphorylations of serine/threonine phosphatases. PP-IIB, or calcineurin (Cn), is a Ca.sup.+2-activated phosphatase; it is involved in the regulation of such diverse cellular functions as ion channel regulation, neuronal transmission, gene transcription, muscle glycogen metabolism, and lymphocyte activation.

PP-IIC is a Mg.sup.++-dependent phosphatase which participates in a wide variety of functions including regulating cyclic AMP-activated protein-phosphatase activity, Ca.sup.++-dependent signal transduction, tRNA splicing, and signal transmission related to heat shock responses. PP-IIC is a monomeric protein with a molecular mass of about 40-45 kDa. One .alpha. and several .beta. isoforms of PP-IIC have been identified (Wenk, J. et al. (1992) FEBS Lett. 297: 135-138; Terasawa, T. et al. (1993) Arch. Biochem. Biophys. 307: 342-349; and Kato, S. et al. (1995) Arch. Biochem. Biophys. 318: 387-393).

The levels of protein phosphorylation required for normal cell growth and differentiation at any time are achieved through the coordinated action of PKs and PPS. Depending on the cellular context, these two types of enzymes may either antagonize or cooperate with each other during signal transduction. An imbalance between these enzymes may impair normal cell functions leading to metabolic disorders and cellular transformation.

For example, insulin binding to the insulin receptor, which is a PTK, triggers a variety of metabolic and growth promoting effects such as glucose transport, biosynthesis of glycogen and fats, DNA synthesis, cell division and differentiation. Diabetes mellitus, which is characterized by insufficient or a lack of insulin signal transduction, can be caused by any abnormality at any step along the insulin signaling pathway. (Olefsky, 1988, in “Cecil Textbook of Medicine,” 18th Ed., 2:1360-81).

It is also well known, for example, that the overexpression of PTKs, such as HER2, can play a decisive role in the development of cancer (Slamon et al., 1987, Science 235:77-82) and that antibodies capable of blocking the activity of this enzyme can abrogate tumor growth (Drebin et al., 1988, Oncogene 2:387-394). Blocking the signal transduction capability of tyrosine phosphatases such as Flk-1 and the PDGF receptor have been shown to block tumor growth in animal models (Millauer et al., 1994, Nature 367:577; Ueno et al., Science, 252:844-848).

Relatively less is known with respect to the direct role of phosphatases in signal transduction; PPs may play a role in human diseases. For example, ectopic expression of RPTP.alpha. produces a transformed phenotype in embryonic fibroblasts (Zheng et al., Nature 359:336-339), and overexpression of RPTP.alpha. in embryonal carcinoma cells causes the cells to differentiate into a cell type with neuronal phenotype (den Hertog et al., EMBO J 12:3789-3798). The gene for human RPTP.gamma. has been localized to chromosome 3p21 which is a segment frequently altered in renal and small lung carcinoma. Mutations may occur in the extracellular segment of RPTP.gamma. which renders a RPTP that no longer respond to external signals (LaForgia et al., Wary et al., 1993, Cancer Res 52:478-482). Mutations in the gene encoding PTP1C (also known as HCP, SHP) are the cause of the moth-eaten phenotype in mice that suffer severe immunodeficiency, and systemic autoimmune disease accompanied by hyperproliferation of macrophages (Schultz et al., 1993, Cell 73:1445-1454). PTP1D (also known as Syp or PTP2C) has been shown to bind through SH2 domains to sites of phosphorylation in PDGFR, EGFR and insulin receptor substrate 1 (IRS-1). Reducing the activity of PTP1D by microinjection of anti-PTP1D antibody has been shown to block insulin or EGF-induced mitogenesis (Xiao et al., 1994, J Biol Chem 269:21244-21248).

The present invention has substantial similarity to protein tyrosine phosphatase (receptor type, Q). It is well established that protein tyrosine phosphorylation plays a key role in regulating structure proteins in migrating cells. Migrating cells interact with the extracellular matrix via focal adhesions (FA), which are contact points that link actin stress fibers to the membrane cytoskeleton and to transmembrane integrins. Engagement of integrins by the extracellular matrix in migrating cells induces tyrosine phosphorylation of several FA components including pp125FAK and paxillin. Specific PTPases have been linked to FA phosphorylation. For example LAR, a broadly expressed receptor PTPase, localizes to FAs in migrating cells but seems to be excluded from developing FAs at extending lamellopodia. This is consistent with a role of this receptor PTPase in FA disassembly by serving to dephosphorylate components that were activated initially by phosphorylation.

The potential importance of PTPases in the glomerulus has been underscored by the recent identification of GLEPP1, a type III receptor-like PTPase (rPTPase), which is localized to the specialized foot processes of the podocyte. GLEPP1 has been proposed to play a role in the regulation of podocyte foot process structure and function. In support of this hypothesis, GLEPP1 protein levels are reduced in several types of human glomerular disease and in several animal models of glomerulonephritis.

Glomerular disease is initiated by a variety of factors, including immunologic, infectious, and toxic agents, as well as hemodynamic processes. A central pathological feature of many types of acute and progressive glomerular disease is injury of mesangial cells, which respond by proliferating as well as by secreting growth factors and extracellular matrix proteins. This contributes to resolution of glomerular damage but may also lead to fibrosis, which occurs in many chronic disease processes. The glomerular mesangial cell is a mesenchymally derived cell that shares properties with fibroblasts and smooth muscle cells and provides structural support to the glomerular tuft.

PTPases play as potential mediators of the mesangial cell response in glomerular disease, because PTPases expressed in the rat anti-Thy 1 model, wherein a new receptor rPTP-GMC1, expressed by glomerular mesangial cells. rPTP-GMC1 is highly restricted to the mesangial cell and that expression is acutely up-regulated in actively proliferating and migrating mesangial cells in the anti-Thy 1 model. rPTP-GMC1 is similar in structure to GLEPP1 and may sense or regulate cell-cell or cell-matrix interactions to mediate glomerular repair. For a review, see Wright et al., J Biol Chem 1998 Sep 11;273(37):23929-37.

The discovery of a new human protein phosphatase and the polynucleotides encoding it satisfies a need in the art by providing new compositions that are useful in the diagnosis, prevention and treatment of biological processes associated with abnormal or unwanted protein phosphorylation.

The present invention is based in part on the identification of amino acid sequences of human phosphatase peptides and proteins that are related to the protein tyrosine phosphatase subfamily, as well as allelic variants and other mammalian orthologs thereof. These unique peptide sequences, and nucleic acid sequences that encode these peptides, can be used as models for the development of human therapeutic targets, aid in the identification of therapeutic proteins, and serve as targets for the development of human therapeutic agents that modulate phosphatase activity in cells and tissues that express the phosphatase. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta.

FIG. 1 provides the nucleotide sequence of a cDNA molecule or transcript sequence that encodes the phosphatase protein of the present invention. (SEQ ID NO:1) In addition, structure and functional information is provided, such as ATG start, stop and tissue distribution, where available, that allows one to readily determine specific uses of inventions based on this molecular sequence. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta.

FIG. 2 provides the predicted amino acid sequence of the phosphatase of the present invention. (SEQ ID NO:2) In addition structure and functional information such as protein family, function, and modification sites is provided where available, allowing one to readily determine specific uses of inventions based on this molecular sequence.

FIG. 3 provides genomic sequences that span the gene encoding the phosphatase protein of the present invention. (SEQ ID NO:3) In addition structure and functional information, such as intron/exon structure, promoter location, etc., is provided where available, allowing one to readily determine specific uses of inventions based on this molecular sequence. 82 SNPs, including 6 indels, have been identified in the gene encoding the phosphatase protein provided by the present invention and are given in FIG. 3 .

General Description

The present invention is based on the sequencing of the human genome. During the sequencing and assembly of the human genome, analysis of the sequence information revealed previously unidentified fragments of the human genome that encode peptides that share structural and/or sequence homology to protein/peptide/domains identified and characterized within the art as being a phosphatase protein or part of a phosphatase protein and are related to the protein tyrosine phosphatase subfamily. Utilizing these sequences, additional genomic sequences were assembled and transcript and/or cDNA sequences were isolated and characterized. Based on this analysis, the present invention provides amino acid sequences of human phosphatase peptides and proteins that are related to the protein tyrosine phosphatase subfamily, nucleic acid sequences in the form of transcript sequences, cDNA sequences and/or genomic sequences that encode these phosphatase peptides and proteins, nucleic acid variation (allelic information), tissue distribution of expression, and information about the closest art known protein/peptide/domain that has structural or sequence homology to the phosphatase of the present invention.

In addition to being previously unknown, the peptides that are provided in the present invention are selected based on their ability to be used for the development of commercially important products and services. Specifically, the present peptides are selected based on homology and/or structural relatedness to known phosphatase proteins of the protein tyrosine phosphatase subfamily and the expression pattern observed. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. The art has clearly established the commercial importance of members of this family of proteins and proteins that have expression patterns similar to that of the present gene. Some of the more specific features of the peptides of the present invention, and the uses thereof, are described herein, particularly in the Background of the Invention and in the annotation provided in the Figures, and/or are known within the art for each of the known protein tyrosine phosphatase family or subfamily of phosphatase proteins.

Specific Embodiments

Peptide Molecules

The present invention provides nucleic acid sequences that encode protein molecules that have been identified as being members of the phosphatase family of proteins and are related to the protein tyrosine phosphatase subfamily (protein sequences are provided in FIG. 2, transcript/cDNA sequences are provided in FIG. 1 and genomic sequences are provided in FIG. 3 ). The peptide sequences provided in FIG. 2 , as well as the obvious variants described herein, particularly allelic variants as identified herein and using the information in FIG. 3, will be referred herein as the phosphatase peptides of the present invention, phosphatase peptides, or peptides/proteins of the present invention.

The present invention provides isolated peptide and protein molecules that consist of, consist essentially of, or comprise the amino acid sequences of the phosphatase peptides disclosed in the FIG. 2, (encoded by the nucleic acid molecule shown in FIG. 1, transcript/cDNA or FIG. 3, genomic sequence), as well as all obvious variants of these peptides that are within the art to make and use. Some of these variants are described in detail below.

As used herein, a peptide is said to be “isolated” or “purified” when it is substantially free of cellular material or free of chemical precursors or other chemicals. The peptides of the present invention can be purified to homogeneity or other degrees of purity. The level of purification will be based on the intended use. The critical feature is that the preparation allows for the desired function of the peptide, even if in the presence of considerable amounts of other components (the features of an isolated nucleic acid molecule is discussed below).

In some uses, “substantially free of cellular material” includes preparations of the peptide having less than about 30% (by dry weight) other proteins (i.e., contaminating protein), less than about 20% other proteins, less than about 10% other proteins, or less than about 5% other proteins. When the peptide is recombinantly produced, it can also be substantially free of culture medium, i.e., culture medium represents less than about 20% of the volume of the protein preparation.

The language “substantially free of chemical precursors or other chemicals” includes preparations of the peptide in which it is separated from chemical precursors or other chemicals that are involved in its synthesis. In one embodiment, the language “substantially free of chemical precursors or other chemicals” includes preparations of the phosphatase peptide having less than about 30% (by dry weight) chemical precursors or other chemicals, less than about 20% chemical precursors or other chemicals, less than about 10% chemical precursors or other chemicals, or less than about 5% chemical precursors or other chemicals.

The isolated phosphatase peptide can be purified from cells that naturally express it, purified from cells that have been altered to express it (recombinant), or synthesized using known protein synthesis methods. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. For example, a nucleic acid molecule encoding the phosphatase peptide is cloned into an expression vector, the expression vector introduced into a host cell and the protein expressed in the host cell. The protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Many of these techniques are described in detail below.

Accordingly, the present invention provides proteins that consist of the amino acid sequences provided in FIG. 2 (SEQ ID NO:2), for example, proteins encoded by the transcript/cDNA nucleic acid sequences shown in FIG. 1 (SEQ ID NO:1) and the genomic sequences provided in FIG. 3 (SEQ ID NO:3). The amino acid sequence of such a protein is provided in FIG. 2. A protein consists of an amino acid sequence when the amino acid sequence is the final amino acid sequence of the protein.

The present invention further provides proteins that consist essentially of the amino acid sequences provided in FIG. 2 (SEQ ID NO:2), for example, proteins encoded by the transcript/cDNA nucleic acid sequences shown in FIG. 1 (SEQ ID NO:1) and the genomic sequences provided in FIG. 3 (SEQ ID NO:3). A protein consists essentially of an amino acid sequence when such an amino acid sequence is present with only a few additional amino acid residues, for example from about 1 to about 100 or so additional residues, typically from 1 to about 20 additional residues in the final protein.

The present invention further provides proteins that comprise the amino acid sequences provided in FIG. 2 (SEQ ID NO:2), for example, proteins encoded by the transcript/cDNA nucleic acid sequences shown in FIG. 1 (SEQ ID NO:1) and the genomic sequences provided in FIG. 3 (SEQ ID NO:3). A protein comprises an amino acid sequence when the amino acid sequence is at least part of the final amino acid sequence of the protein. In such a fashion, the protein can be only the peptide or have additional amino acid molecules, such as amino acid residues (contiguous encoded sequence) that are naturally associated with it or heterologous amino acid residues/peptide sequences. Such a protein can have a few additional amino acid residues or can comprise several hundred or more additional amino acids. The preferred classes of proteins that are comprised of the phosphatase peptides of the present invention are the naturally occurring mature proteins. A brief description of how various types of these proteins can be made/isolated is provided below.

The phosphatase peptides of the present invention can be attached to heterologous sequences to form chimeric or fusion proteins. Such chimeric and fusion proteins comprise a phosphatase peptide operatively linked to a heterologous protein having an amino acid sequence not substantially homologous to the phosphatase peptide. “Operatively linked” indicates that the phosphatase peptide and the heterologous protein are fused in-frame. The heterologous protein can be fused to the N-terminus or C-terminus of the phosphatase peptide.

In some uses, the fusion protein does not affect the activity of the phosphatase peptide per se. For example, the fusion protein can include, but is not limited to, enzymatic fusion proteins, for example beta-galactosidase fusions, yeast two-hybrid GAL fusions, poly-His fusions, MYC-tagged, HI-tagged and Ig fusions. Such fusion proteins, particularly poly-His fusions, can facilitate the purification of recombinant phosphatase peptide. In certain host cells (e.g., mammalian host cells), expression and/or secretion of a protein can be increased by using a heterologous signal sequence.

A chimeric or fusion protein can be produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different protein sequences are ligated together in-frame in accordance with conventional techniques. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers. Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and re-amplified to generate a chimeric gene sequence (see Ausubel et al., Current Protocols in Molecular Biology, 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety (e.g., a GST protein). A phosphatase peptide-encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the phosphatase peptide.

As mentioned above, the present invention also provides and enables obvious variants of the amino acid sequence of the proteins of the present invention, such as naturally occurring mature forms of the peptide, allelic/sequence variants of the peptides, non-naturally occurring recombinantly derived variants of the peptides, and orthologs and paralogs of the peptides. Such variants can readily be generated using art-known techniques in the fields of recombinant nucleic acid technology and protein biochemistry. It is understood, however, that variants exclude any amino acid sequences disclosed prior to the invention.

Such variants can readily be identified/made using molecular techniques and the sequence information disclosed herein. Further, such variants can readily be distinguished from other peptides based on sequence and/or structural homology to the phosphatase peptides of the present invention. The degree of homology/identity present will be based primarily on whether the peptide is a functional variant or non-functional variant, the amount of divergence present in the paralog family and the evolutionary distance between the orthologs.

To determine the percent identity of two amino acid sequences or two nucleic acid sequences, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in one or both of a first and a second amino acid or nucleic acid sequence for optimal alignment and non-homologous sequences can be disregarded for comparison purposes). In a preferred embodiment, at least 30%, 40%, 50%, 60%, 70%, 80%, or 90% or more of the length of a reference sequence is aligned for comparison purposes. The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position (as used herein amino acid or nucleic acid “identity” is equivalent to amino acid or nucleic acid “homology”). The percent identity between the two sequences is a function of the number of identical positions shared by the sequences, taking into account the number of gaps, and the length of each gap, which need to be introduced for optimal alignment of the two sequences.

The comparison of sequences and determination of percent identity and similarity between two sequences can be accomplished using a mathematical algorithm. ( Computational Molecular Biology, Lesk, A. M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects , Smith, D. W., ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part 1, Griffin, A. M., and Griffin, H. G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heinje, G., Academic Press, 1987; and Sequence Analysis Primer, Gribskov, M. and Devereux, J., eds., M Stockton Press, New York, 1991). In a preferred embodiment, the percent identity between two amino acid sequences is determined using the Needleman and Wunsch ( J. Mol. Biol . (48):444-453 (1970)) algorithm which has been incorporated into the GAP program in the GCG software package (available at http://www.gcg.com), using either a Blossom 62 matrix or a PAM250 matrix, and a gap weight of 16, 14, 12, 10, 8, 6, or 4 and a length weight of 1, 2, 3, 4, 5, or 6. In yet another preferred embodiment, the percent identity between two nucleotide sequences is determined using the GAP program in the GCG software package (Devereux, J., et al., Nucleic Acids Res. 12(1):387 (1984)) (available at http://www.gcg.com), using a NWSgapdna.CMP matrix and a gap weight of 40, 50, 60, 70, or 80 and a length weight of 1, 2, 3, 4, 5, or 6. In another embodiment, the percent identity between two amino acid or nucleotide sequences is determined using the algorithm of E. Myers and W. Miller (CABIOS, 4:11-17 (1989)) which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4.

The nucleic acid and protein sequences of the present invention can further be used as a “query sequence” to perform a search against sequence databases to, for example, identify other family members or related sequences. Such searches can be performed using the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. ( J. Mol. Biol. 215:403-10 (1990)). BLAST nucleotide searches can be performed with the NBLAST program, score=100, wordlength=12 to obtain nucleotide sequences homologous to the nucleic acid molecules of the invention. BLAST protein searches can be performed with the XBLAST program, score=50, wordlength=3 to obtain amino acid sequences homologous to the proteins of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al. ( Nucleic Acids Res. 25(17):3389-3402 (1997)). When utilizing BLAST and gapped BLAST programs, the default parameters of the respective programs (e.g., XBLAST and NBLAST) can be used.

Full-length pre-processed forms, as well as mature processed forms, of proteins that comprise one of the peptides of the present invention can readily be identified as having complete sequence identity to one of the phosphatase peptides of the present invention as well as being encoded by the same genetic locus as the phosphatase peptide provided herein. As indicated by the data presented in FIG. 3, the map position was determined to be on chromosome 12 by ePCR.

Allelic variants of a phosphatase peptide can readily be identified as being a human protein having a high degree (significant) of sequence homology/identity to at least a portion of the phosphatase peptide as well as being encoded by the same genetic locus as the phosphatase peptide provided herein. Genetic locus can readily be determined based on the genomic information provided in FIG. 3, such as the genomic sequence mapped to the reference human. As indicated by the data presented in FIG. 3, the map position was determined to be on chromosome 12 by ePCR. As used herein, two proteins (or a region of the proteins) have significant homology when the amino acid sequences are typically at least about 70-80%, 80-90%, and more typically at least about 90-95% or more homologous. A significantly homologous amino acid sequence, according to the present invention, will be encoded by a nucleic acid sequence that will hybridize to a phosphatase peptide encoding nucleic acid molecule under stringent conditions as more fully described below.

FIG. 3 provides information on SNPs that have been identified in a gene encoding the phosphatase protein of the present invention. 82 SNP variants were found, including 6 indels (indicated by a “-”) and 3 SNPs in exons, of which 3 of these cause changes in the amino acid sequence (i.e., nonsynonymous SNPs). SNPs, identified at different nucleotide positions in introns and regions 5′ and 3′ of the ORF, may affect control/regulatory elements.

Paralogs of a phosphatase peptide can readily be identified as having some degree of significant sequence homology/identity to at least a portion of the phosphatase peptide, as being encoded by a gene from humans, and as having similar activity or function. Two proteins will typically be considered paralogs when the amino acid sequences are typically at least about 60% or greater, and more typically at least about 70% or greater homology through a given region or domain. Such paralogs will be encoded by a nucleic acid sequence that will hybridize to a phosphatase peptide encoding nucleic acid molecule under moderate to stringent conditions as more fully described below.

Orthologs of a phosphatase peptide can readily be identified as having some degree of significant sequence homology/identity to at least a portion of the phosphatase peptide as well as being encoded by a gene from another organism. Preferred orthologs will be isolated from mammals, preferably primates, for the development of human therapeutic targets and agents. Such orthologs will be encoded by a nucleic acid sequence that will hybridize to a phosphatase peptide encoding nucleic acid molecule under moderate to stringent conditions, as more fully described below, depending on the degree of relatedness of the two organisms yielding the proteins.

Non-naturally occurring variants of the phosphatase peptides of the present invention can readily be generated using recombinant techniques. Such variants include, but are not limited to deletions, additions and substitutions in the amino acid sequence of the phosphatase peptide. For example, one class of substitutions are conserved amino acid substitution. Such substitutions are those that substitute a given amino acid in a phosphatase peptide by another amino acid of like characteristics. Typically seen as conservative substitutions are the replacements, one for another, among the aliphatic amino acids Ala, Val, Leu, and Ile; interchange of the hydroxyl residues Ser and Thr; exchange of the acidic residues Asp and Glu; substitution between the amide residues Asn and Gln; exchange of the basic residues Lys and Arg; and replacements among the aromatic residues Phe and Tyr. Guidance concerning which amino acid changes are likely to be phenotypically silent are found in Bowie et al., Science 247:1306-1310 (1990).

Variant phosphatase peptides can be fully functional or can lack function in one or more activities, e.g. ability to bind substrate, ability to dephosphorylate substrate, ability to mediate signaling, etc. Fully functional variants typically contain only conservative variation or variation in non-critical residues or in non-critical regions. FIG. 2 provides the result of protein analysis and can be used to identify critical domains/regions. Functional variants can also contain substitution of similar amino acids that result in no change or an insignificant change in function. Alternatively, such substitutions may positively or negatively affect function to some degree.

Non-functional variants typically contain one or more non-conservative amino acid substitutions, deletions, insertions, inversions, or truncation or a substitution, insertion, inversion, or deletion in a critical residue or critical region.

Amino acids that are essential for function can be identified by methods known in the art, such as site-directed mutagenesis or alanine-scanning mutagenesis (Cunningham et al., Science 244:1081-1085 (1989)), particularly using the results provided in FIG. 2 . The latter procedure introduces single alanine mutations at every residue in the molecule. The resulting mutant molecules are then tested for biological activity such as phosphatase activity or in assays such as an in vitro proliferative activity. Sites that are critical for binding partner/substrate binding can also be determined by structural analysis such as crystallization, nuclear magnetic resonance or photoaffinity labeling (Smith et al., J. Mol. Biol. 224:899-904 (1992); de Vos et al. Science 255:306-312 (1992)).

The present invention further provides fragments of the phosphatase peptides, in addition to proteins and peptides that comprise and consist of such fragments, particularly those comprising the residues identified in FIG. 2 . The fragments to which the invention pertains, however, are not to be construed as encompassing fragments that may be disclosed publicly prior to the present invention.

As used herein, a fragment comprises at least 8, 10, 12, 14, 16, or more contiguous amino acid residues from a phosphatase peptide. Such fragments can be chosen based on the ability to retain one or more of the biological activities of the phosphatase peptide or could be chosen for the ability to perform a function, e.g. bind a substrate or act as an immunogen. Particularly important fragments are biologically active fragments, peptides that are, for example, about 8 or more amino acids in length. Such fragments will typically comprise a domain or motif of the phosphatase peptide, e.g., active site, a transmembrane domain or a substrate-binding domain. Further, possible fragments include, but are not limited to, domain or motif containing fragments, soluble peptide fragments, and fragments containing immunogenic structures. Predicted domains and functional sites are readily identifiable by computer programs well known and readily available to those of skill in the art (e.g., PROSITE analysis). The results of one such analysis are provided in FIG. 2 .

Polypeptides often contain amino acids other than the 20 amino acids commonly referred to as the 20 naturally occurring amino acids. Further, many amino acids, including the terminal amino acids, may be modified by natural processes, such as processing and other post-translational modifications, or by chemical modification techniques well known in the art. Common modifications that occur naturally in phosphatase peptides are described in basic texts, detailed monographs, and the research literature, and they are well known to those of skill in the art (some of these features are identified in FIG. 2 ).

Known modifications include, but are not limited to, acetylation, acylation, ADP-ribosylation, amidation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent crosslinks, formation of cystine, formation of pyroglutamate, formylation, gamma carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic processing, phosphorylation, prenylation, racemization, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins such as arginylation, and ubiquitination.

Such modifications are well known to those of skill in the art and have been described in great detail in the scientific literature. Several particularly common modifications, glycosylation, lipid attachment, sulfation, gamma-carboxylation of glutamic acid residues, hydroxylation and ADP-ribosylation, for instance, are described in most basic texts, such as Proteins—Structure and Molecular Properties, 2nd Ed., T. E. Creighton, W. H. Freeman and Company, New York (1993). Many detailed reviews are available on this subject, such as by Wold, F., Posttranslational Covalent Modification of Proteins , B. C. Johnson, Ed., Academic Press, New York 1-12 (1983); Seifter et al. ( Meth. Enzymol. 182: 626-646 (1990)) and Rattan et al. ( Ann. N.Y. Acad. Sci. 663:48-62 (1992)).

Accordingly, the phosphatase peptides of the present invention also encompass derivatives or analogs in which a substituted amino acid residue is not one encoded by the genetic code, in which a substituent group is included, in which the mature phosphatase peptide is fused with another compound, such as a compound to increase the half-life of the phosphatase peptide, or in which the additional amino acids are fused to the mature phosphatase peptide, such as a leader or secretory sequence or a sequence for purification of the mature phosphatase peptide or a pro-protein sequence.

Protein/Peptide Uses

The proteins of the present invention can be used in substantial and specific assays related to the functional information provided in the Figures; to raise antibodies or to elicit another immune response; as a reagent (including the labeled reagent) in assays designed to quantitatively determine levels of the protein (or its binding partner or ligand) in biological fluids; and as markers for tissues in which the corresponding protein is preferentially expressed (either constitutively or at a particular stage of tissue differentiation or development or in a disease state). Where the protein binds or potentially binds to another protein or ligand (such as, for example, in a phosphatase-effector protein interaction or phosphatase-ligand interaction), the protein can be used to identify the binding partner/ligand so as to develop a system to identify inhibitors of the binding interaction. Any or all of these uses are capable of being developed into reagent grade or kit format for commercialization as commercial products.

Methods for performing the uses listed above are well known to those skilled in the art. References disclosing such methods include “Molecular Cloning: A Laboratory Manual”, 2d ed., Cold Spring Harbor Laboratory Press, Sambrook, J., E. F. Fritsch and T. Maniatis eds., 1989, and “Methods in Enzymology: Guide to Molecular Cloning Techniques”, Academic Press, Berger, S. L. and A. R. Kimmel eds., 1987.

The potential uses of the peptides of the present invention are based primarily on the source of the protein as well as the class/action of the protein. For example, phosphatases isolated from humans and their human/mammalian orthologs serve as targets for identifying agents for use in mammalian therapeutic applications, e.g. a human drug, particularly in modulating a biological or pathological response in a cell or tissue that expresses the phosphatase. Experimental data as provided in FIG. 1 indicates that phosphatase proteins of the present invention are expressed in the colon adenocarcinoma detected by a virtual northern blot. In addition, PCR-based tissue screening panel indicates expression in and placenta. A large percentage of pharmaceutical agents are being developed that modulate the activity of phosphatase proteins, particularly members of the protein tyrosine phosphatase subfamily (see Background of the Invention). The structural and functional information provided in the Background and Figures provide specific and substantial uses for the molecules of the present invention, particularly in combination with the expression information provided in FIG. 1 . Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. Such uses can readily be determined using the information provided herein, that which is known in the art, and routine experimentation.

The proteins of the present invention (including variants and fragments that may have been disclosed prior to the present invention) are useful for biological assays related to phosphatases that are related to members of the protein tyrosine phosphatase subfamily. Such assays involve any of the known phosphatase functions or activities or properties useful for diagnosis and treatment of phosphatase-related conditions that are specific for the subfamily of phosphatases that the one of the present invention belongs to, particularly in cells and tissues that express the phosphatase. Experimental data as provided in FIG. 1 indicates that phosphatase proteins of the present invention are expressed in the colon adenocarcinoma detected by a virtual northern blot. In addition, PCR-based tissue screening panel indicates expression in and placenta.

The proteins of the present invention are also useful in drug screening assays, in cell-based or cell-free systems. Cell-based systems can be native, i.e., cells that normally express the phosphatase, as a biopsy or expanded in cell culture. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. In an alternate embodiment, cell-based assays involve recombinant host cells expressing the phosphatase protein.

The polypeptides can be used to identify compounds that modulate phosphatase activity of the protein in its natural state or an altered form that causes a specific disease or pathology associated with the phosphatase. Both the phosphatases of the present invention and appropriate variants and fragments can be used in high-throughput screens to assay candidate compounds for the ability to bind to the phosphatase. These compounds can be further screened against a functional phosphatase to determine the effect of the compound on the phosphatase activity. Further, these compounds can be tested in animal or invertebrate systems to determine activity/effectiveness. Compounds can be identified that activate (agonist) or inactivate (antagonist) the phosphatase to a desired degree.

Further, the proteins of the present invention can be used to screen a compound for the ability to stimulate or inhibit interaction between the phosphatase protein and a molecule that normally interacts with the phosphatase protein, e.g. a substrate or a component of the signal pathway that the phosphatase protein normally interacts (for example, another phosphatase). Such assays typically include the steps of combining the phosphatase protein with a candidate compound under conditions that allow the phosphatase protein, or fragment, to interact with the target molecule, and to detect the formation of a complex between the protein and the target or to detect the biochemical consequence of the interaction with the phosphatase protein and the target, such as any of the associated effects of signal transduction such as protein phosphorylation, cAMP turnover, and adenylate cyclase activation, etc.

Candidate compounds include, for example, 1) peptides such as soluble peptides, including Ig-tailed fusion peptides and members of random peptide libraries (see, e.g., Lam et al., Nature 354:82-84 (1991); Houghten et al., Nature 354:84-86 (1991)) and combinatorial chemistry-derived molecular libraries made of D- and/or L-configuration amino acids; 2) phosphopeptides (e.g., members of random and partially degenerate, directed phosphopeptide libraries, see, e.g., Songyang et al., Cell 72:767-778 (1993)); 3) antibodies (e.g., polyclonal, monoclonal, humanized, anti-idiotypic, chimeric, and single chain antibodies as well as Fab, F(ab′) 2 , Fab expression library fragments, and epitope-binding fragments of antibodies); and 4) small organic and inorganic molecules (e.g., molecules obtained from combinatorial and natural product libraries).

One candidate compound is a soluble fragment of the receptor that competes for substrate binding. Other candidate compounds include mutant phosphatases or appropriate fragments containing mutations that affect phosphatase function and thus compete for substrate. Accordingly, a fragment that competes for substrate, for example with a higher affinity, or a fragment that binds substrate but does not allow release, is encompassed by the invention.

The invention further includes other end point assays to identify compounds that modulate (stimulate or inhibit) phosphatase activity. The assays typically involve an assay of events in the signal transduction pathway that indicate phosphatase activity. Thus, the dephosphorylation of a substrate, activation of a protein, a change in the expression of genes that are up- or down-regulated in response to the phosphatase protein dependent signal cascade can be assayed.

Any of the biological or biochemical functions mediated by the phosphatase can be used as an endpoint assay. These include all of the biochemical or biochemical/biological events described herein, in the references cited herein, incorporated by reference for these endpoint assay targets, and other functions known to those of ordinary skill in the art or that can be readily identified using the information provided in the Figures, particularly FIG. 2 . Specifically, a biological function of a cell or tissues that expresses the phosphatase can be assayed. Experimental data as provided in FIG. 1 indicates that phosphatase proteins of the present invention are expressed in the colon adenocarcinoma detected by a virtual northern blot. In addition, PCR-based tissue screening panel indicates expression in and placenta.

Binding and/or activating compounds can also be screened by using chimeric phosphatase proteins in which the amino terminal extracellular domain, or parts thereof, the entire transmembrane domain or subregions, such as any of the seven transmembrane segments or any of the intracellular or extracellular loops and the carboxy terminal intracellular domain, or parts thereof, can be replaced by heterologous domains or subregions. For example, a substrate-binding region can be used that interacts with a different substrate then that which is recognized by the native phosphatase. Accordingly, a different set of signal transduction components is available as an end-point assay for activation. This allows for assays to be performed in other than the specific host cell from which the phosphatase is derived.

The proteins of the present invention are also useful in competition binding assays in methods designed to discover compounds that interact with the phosphatase (e.g. binding partners and/or ligands). Thus, a compound is exposed to a phosphatase polypeptide under conditions that allow the compound to bind or to otherwise interact with the polypeptide. Soluble phosphatase polypeptide is also added to the mixture. If the test compound interacts with the soluble phosphatase polypeptide, it decreases the amount of complex formed or activity from the phosphatase target. This type of assay is particularly useful in cases in which compounds are sought that interact with specific regions of the phosphatase. Thus, the soluble polypeptide that competes with the target phosphatase region is designed to contain peptide sequences corresponding to the region of interest.

To perform cell free drug screening assays, it is sometimes desirable to immobilize either the phosphatase protein, or fragment, or its target molecule to facilitate separation of complexes from uncomplexed forms of one or both of the proteins, as well as to accommodate automation of the assay.

Techniques for immobilizing proteins on matrices can be used in the drug screening assays. In one embodiment, a fusion protein can be provided which adds a domain that allows the protein to be bound to a matrix. For example, glutathione-S-transferase fusion proteins can be adsorbed onto glutathione sepharose beads (Sigma Chemical, St. Louis, Mo.) or glutathione derivatized microtitre plates, which are then combined with the cell lysates (e.g., 35 S-labeled) and the candidate compound, and the mixture incubated under conditions conducive to complex formation (e.g., at physiological conditions for salt and pH). Following incubation, the beads are washed to remove any unbound label, and the matrix immobilized and radiolabel determined directly, or in the supernatant after the complexes are dissociated. Alternatively, the complexes can be dissociated from the matrix, separated by SDS-PAGE, and the level of phosphatase-binding protein found in the bead fraction quantitated from the gel using standard electrophoretic techniques. For example, either the polypeptide or its target molecule can be immobilized utilizing conjugation of biotin and streptavidin using techniques well known in the art. Alternatively, antibodies reactive with the protein but which do not interfere with binding of the protein to its target molecule can be derivatized to the wells of the plate, and the protein trapped in the wells by antibody conjugation. Preparations of a phosphatase-binding protein and a candidate compound are incubated in the phosphatase protein-presenting wells and the amount of complex trapped in the well can be quantitated. Methods for detecting such complexes, in addition to those described above for the GST-immobilized complexes, include immunodetection of complexes using antibodies reactive with the phosphatase protein target molecule, or which are reactive with phosphatase protein and compete with the target molecule, as well as enzyme-linked assays which rely on detecting an enzymatic activity associated with the target molecule.

Agents that modulate one of the phosphatases of the present invention can be identified using one or more of the above assays, alone or in combination. It is generally preferable to use a cell-based or cell free system first and then confirm activity in an animal or other model system. Such model systems are well known in the art and can readily be employed in this context.

Modulators of phosphatase protein activity identified according to these drug screening assays can be used to treat a subject with a disorder mediated by the kinase pathway, by treating cells or tissues that express the phosphatase. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. These methods of treatment include the steps of administering a modulator of phosphatase activity in a pharmaceutical composition to a subject in need of such treatment, the modulator being identified as described herein.

In yet another aspect of the invention, the phosphatase proteins can be used as “bait proteins” in a two-hybrid assay or three-hybrid assay (see, e.g., U.S. Pat. No. 5,283,317; Zervos et al. (1993) Cell 72:223-232; Madura et al. (1993) J. Biol. Chem. 268:12046-12054; Bartel et al. (1993) Biotechniques 14:920-924; Iwabuchi et al. (1993) Oncogene 8:1693-1696; and Brent WO94/10300), to identify other proteins, which bind to or interact with the phosphatase and are involved in phosphatase activity. Such phosphatase-binding proteins are also likely to be involved in the propagation of signals by the phosphatase proteins or phosphatase targets as, for example, downstream elements of a kinase-mediated signaling pathway. Alternatively, such phosphatase-binding proteins are likely to be phosphatase inhibitors.

The two-hybrid system is based on the modular nature of most transcription factors, which consist of separable DNA-binding and activation domains. Briefly, the assay utilizes two different DNA constructs. In one construct, the gene that codes for a phosphatase protein is fused to a gene encoding the DNA binding domain of a known transcription factor (e.g., GAL-4). In the other construct, a DNA sequence, from a library of DNA sequences, that encodes an unidentified protein (“prey” or “sample”) is fused to a gene that codes for the activation domain of the known transcription factor. If the “bait” and the “prey” proteins are able to interact, in vivo, forming a phosphatase-dependent complex, the DNA-binding and activation domains of the transcription factor are brought into close proximity. This proximity allows transcription of a reporter gene (e.g., LacZ) which is operably linked to a transcriptional regulatory site responsive to the transcription factor. Expression of the reporter gene can be detected and cell colonies containing the functional transcription factor can be isolated and used to obtain the cloned gene which encodes the protein which interacts with the phosphatase protein.

This invention further pertains to novel agents identified by the above-described screening assays. Accordingly, it is within the scope of this invention to further use an agent identified as described herein in an appropriate animal model. For example, an agent identified as described herein (e.g., a phosphatase-modulating agent, an antisense phosphatase nucleic acid molecule, a phosphatase-specific antibody, or a phosphatase-binding partner) can be used in an animal or other model to determine the efficacy, toxicity, or side effects of treatment with such an agent. Alternatively, an agent identified as described herein can be used in an animal or other model to determine the mechanism of action of such an agent. Furthermore, this invention pertains to uses of novel agents identified by the above-described screening assays for treatments as described herein.

The phosphatase proteins of the present invention are also useful to provide a target for diagnosing a disease or predisposition to disease mediated by the peptide. Accordingly, the invention provides methods for detecting the presence, or levels of, the protein (or encoding mRNA) in a cell, tissue, or organism. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. The method involves contacting a biological sample with a compound capable of interacting with the phosphatase protein such that the interaction can be detected. Such an assay can be provided in a single detection format or a multi-detection format such as an antibody chip array.

One agent for detecting a protein in a sample is an antibody capable of selectively binding to protein. A biological sample includes tissues, cells and biological fluids isolated from a subject, as well as tissues, cells and fluids present within a subject.

The peptides of the present invention also provide targets for diagnosing active protein activity, disease, or predisposition to disease, in a patient having a variant peptide, particularly activities and conditions that are known for other members of the family of proteins to which the present one belongs. Thus, the peptide can be isolated from a biological sample and assayed for the presence of a genetic mutation that results in aberrant peptide. This includes amino acid substitution, deletion, insertion, rearrangement, (as the result of aberrant splicing events), and inappropriate post-translational modification. Analytic methods include altered electrophoretic mobility, altered tryptic peptide digest, altered phosphatase activity in cell-based or cell-free assay, alteration in substrate or antibody-binding pattern, altered isoelectric point, direct amino acid sequencing, and any other of the known assay techniques useful for detecting mutations in a protein. Such an assay can be provided in a single detection format or a multi-detection format such as an antibody chip array.

In vitro techniques for detection of peptide include enzyme linked immunosorbent assays (ELISAs), Western blots, immunoprecipitations and immunofluorescence using a detection reagent, such as an antibody or protein binding agent. Alternatively, the peptide can be detected in vivo in a subject by introducing into the subject a labeled anti-peptide antibody or other types of detection agent. For example, the antibody can be labeled with a radioactive marker whose presence and location in a subject can be detected by standard imaging techniques. Particularly useful are methods that detect the allelic variant of a peptide expressed in a subject and methods which detect fragments of a peptide in a sample.

The peptides are also useful in pharmacogenomic analysis. Pharmacogenomics deal with clinically significant hereditary variations in the response to drugs due to altered drug disposition and abnormal action in affected persons. See, e.g., Eichelbaum, M. ( Clin. Exp. Pharmacol. Physiol. 23(10-11):983-985 (1996)), and Linder, M. W. ( Clin. Chem. 43(2):254-266 (1997)). The clinical outcomes of these variations result in severe toxicity of therapeutic drugs in certain individuals or therapeutic failure of drugs in certain individuals as a result of individual variation in metabolism. Thus, the genotype of the individual can determine the way a therapeutic compound acts on the body or the way the body metabolizes the compound. Further, the activity of drug metabolizing enzymes effects both the intensity and duration of drug action. Thus, the pharmacogenomics of the individual permit the selection of effective compounds and effective dosages of such compounds for prophylactic or therapeutic treatment based on the individual's genotype. The discovery of genetic polymorphisms in some drug metabolizing enzymes has explained why some patients do not obtain the expected drug effects, show an exaggerated drug effect, or experience serious toxicity from standard drug dosages. Polymorphisms can be expressed in the phenotype of the extensive metabolizer and the phenotype of the poor metabolizer. Accordingly, genetic polymorphism may lead to allelic protein variants of the phosphatase protein in which one or more of the phosphatase functions in one population is different from those in another population. The peptides thus allow a target to ascertain a genetic predisposition that can affect treatment modality. Thus, in a ligand-based treatment, polymorphism may give rise to amino terminal extracellular domains and/or other substrate-binding regions that are more or less active in substrate binding, and phosphatase activation. Accordingly, substrate dosage would necessarily be modified to maximize the therapeutic effect within a given population containing a polymorphism. As an alternative to genotyping, specific polymorphic peptides could be identified.

The peptides are also useful for treating a disorder characterized by an absence of, inappropriate, or unwanted expression of the protein. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. Accordingly, methods for treatment include the use of the phosphatase protein or fragments.

Antibodies

The invention also provides antibodies that selectively bind to one of the peptides of the present invention, a protein comprising such a peptide, as well as variants and fragments thereof. As used herein, an antibody selectively binds a target peptide when it binds the target peptide and does not significantly bind to unrelated proteins. An antibody is still considered to selectively bind a peptide even if it also binds to other proteins that are not substantially homologous with the target peptide so long as such proteins share homology with a fragment or domain of the peptide target of the antibody. In this case, it would be understood that antibody binding to the peptide is still selective despite some degree of cross-reactivity.

As used herein, an antibody is defined in terms consistent with that recognized within the art: they are multi-subunit proteins produced by a mammalian organism in response to an antigen challenge. The antibodies of the present invention include polyclonal antibodies and monoclonal antibodies, as well as fragments of such antibodies, including, but not limited to, Fab or F(ab′) 2 , and Fv fragments.

Many methods are known for generating and/or identifying antibodies to a given target peptide. Several such methods are described by Harlow, Antibodies, Cold Spring Harbor Press, (1989).

In general, to generate antibodies, an isolated peptide is used as an immunogen and is administered to a mammalian organism, such as a rat, rabbit or mouse. The full-length protein, an antigenic peptide fragment or a fusion protein can be used. Particularly important fragments are those covering functional domains, such as the domains identified in FIG. 2, and domain of sequence homology or divergence amongst the family, such as those that can readily be identified using protein alignment methods and as presented in the Figures.

Antibodies are preferably prepared from regions or discrete fragments of the phosphatase proteins. Antibodies can be prepared from any region of the peptide as described herein. However, preferred regions will include those involved in function/activity and/or phosphatase/binding partner interaction. FIG. 2 can be used to identify particularly important regions while sequence alignment can be used to identify conserved and unique sequence fragments.

An antigenic fragment will typically comprise at least 8 contiguous amino acid residues. The antigenic peptide can comprise, however, at least 10, 12, 14, 16 or more amino acid residues. Such fragments can be selected on a physical property, such as fragments correspond to regions that are located on the surface of the protein, e.g., hydrophilic regions or can be selected based on sequence uniqueness (see FIG. 2 ).

Detection on an antibody of the present invention can be facilitated by coupling (i.e., physically linking) the antibody to a detectable substance. Examples of detectable substances include various enzymes, prosthetic groups, fluorescent materials, luminescent materials, bioluminescent materials, and radioactive materials. Examples of suitable enzymes include horseradish peroxidase, alkaline phosphatase, β-galactosidase, or acetylcholinesterase; examples of suitable prosthetic group complexes include streptavidin/biotin and avidin/biotin; examples of suitable fluorescent materials include umbelliferone, fluorescein, fluorescein isothiocyanate, rhodamine, dichlorotriazinylamine fluorescein, dansyl chloride or phycoerythrin; an example of a luminescent material includes luminol; examples of bioluminescent materials include luciferase, luciferin, and aequorin, and examples of suitable radioactive material include 125 I, 131 I, 35 S or 3 H.

Antibody Uses

The antibodies can be used to isolate one of the proteins of the present invention by standard techniques, such as affinity chromatography or immunoprecipitation. The antibodies can facilitate the purification of the natural protein from cells and recombinantly produced protein expressed in host cells. In addition, such antibodies are useful to detect the presence of one of the proteins of the present invention in cells or tissues to determine the pattern of expression of the protein among various tissues in an organism and over the course of normal development. Experimental data as provided in FIG. 1 indicates that phosphatase proteins of the present invention are expressed in the colon adenocarcinoma detected by a virtual northern blot. In addition, PCR-based tissue screening panel indicates expression in and placenta. Further, such antibodies can be used to detect protein in situ, in vitro, or in a cell lysate or supernatant in order to evaluate the abundance and pattern of expression. Also, such antibodies can be used to assess abnormal tissue distribution or abnormal expression during development or progression of a biological condition. Antibody detection of circulating fragments of the full length protein can be used to identify turnover.

Further, the antibodies can be used to assess expression in disease states such as in active stages of the disease or in an individual with a predisposition toward disease related to the protein's function. When a disorder is caused by an inappropriate tissue distribution, developmental expression, level of expression of the protein, or expressed/processed form, the antibody can be prepared against the normal protein. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. If a disorder is characterized by a specific mutation in the protein, antibodies specific for this mutant protein can be used to assay for the presence of the specific mutant protein.

The antibodies can also be used to assess normal and aberrant subcellular localization of cells in the various tissues in an organism. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. The diagnostic uses can be applied, not only in genetic testing, but also in monitoring a treatment modality. Accordingly, where treatment is ultimately aimed at correcting expression level or the presence of aberrant sequence and aberrant tissue distribution or developmental expression, antibodies directed against the protein or relevant fragments can be used to monitor therapeutic efficacy.

Additionally, antibodies are useful in pharmacogenomic analysis. Thus, antibodies prepared against polymorphic proteins can be used to identify individuals that require modified treatment modalities. The antibodies are also useful as diagnostic tools as an immunological marker for aberrant protein analyzed by electrophoretic mobility, isoelectric point, tryptic peptide digest, and other physical assays known to those in the art.

The antibodies are also useful for tissue typing. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. Thus, where a specific protein has been correlated with expression in a specific tissue, antibodies that are specific for this protein can be used to identify a tissue type.

The antibodies are also useful for inhibiting protein function, for example, blocking the binding of the phosphatase peptide to a binding partner such as a substrate. These uses can also be applied in a therapeutic context in which treatment involves inhibiting the protein's function. An antibody can be used, for example, to block binding, thus modulating (agonizing or antagonizing) the peptides activity. Antibodies can be prepared against specific fragments containing sites required for function or against intact protein that is associated with a cell or cell membrane. See FIG. 2 for structural information relating to the proteins of the present invention.

The invention also encompasses kits for using antibodies to detect the presence of a protein in a biological sample. The kit can comprise antibodies such as a labeled or labelable antibody and a compound or agent for detecting protein in a biological sample; means for determining the amount of protein in the sample; means for comparing the amount of protein in the sample with a standard; and instructions for use. Such a kit can be supplied to detect a single protein or epitope or can be configured to detect one of a multitude of epitopes, such as in an antibody detection array. Arrays are described in detail below for nuleic acid arrays and similar methods have been developed for antibody arrays.

Nucleic Acid Molecules

The present invention further provides isolated nucleic acid molecules that encode a phosphatase peptide or protein of the present invention (cDNA, transcript and genomic sequence). Such nucleic acid molecules will consist of, consist essentially of, or comprise a nucleotide sequence that encodes one of the phosphatase peptides of the present invention, an allelic variant thereof, or an ortholog or paralog thereof.

As used herein, an “isolated” nucleic acid molecule is one that is separated from other nucleic acid present in the natural source of the nucleic acid. Preferably, an “isolated” nucleic acid is free of sequences which naturally flank the nucleic acid (i.e., sequences located at the 5′ and 3′ ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. However, there can be some flanking nucleotide sequences, for example up to about 5KB, 4KB, 3KB, 2KB, or 1KB or less, particularly contiguous peptide encoding sequences and peptide encoding sequences within the same gene but separated by introns in the genomic sequence. The important point is that the nucleic acid is isolated from remote and unimportant flanking sequences such that it can be subjected to the specific manipulations described herein such as recombinant expression, preparation of probes and primers, and other uses specific to the nucleic acid sequences.

Moreover, an “isolated” nucleic acid molecule, such as a transcript/cDNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized. However, the nucleic acid molecule can be fused to other coding or regulatory sequences and still be considered isolated.

For example, recombinant DNA molecules contained in a vector are considered isolated. Further examples of isolated DNA molecules include recombinant DNA molecules maintained in heterologous host cells or purified (partially or substantially) DNA molecules in solution. Isolated RNA molecules include in vivo or in vitro RNA transcripts of the isolated DNA molecules of the present invention. Isolated nucleic acid molecules according to the present invention further include such molecules produced synthetically.

Accordingly, the present invention provides nucleic acid molecules that consist of the nucleotide sequence shown in FIG. 1 or 3 (SEQ ID NO:1, transcript sequence and SEQ ID NO:3, genomic sequence), or any nucleic acid molecule that encodes the protein provided in FIG. 2, SEQ ID NO:2. A nucleic acid molecule consists of a nucleotide sequence when the nucleotide sequence is the complete nucleotide sequence of the nucleic acid molecule.

The present invention further provides nucleic acid molecules that consist essentially of the nucleotide sequence shown in FIG. 1 or 3 (SEQ ID NO:1, transcript sequence and SEQ ID NO:3, genomic sequence), or any nucleic acid molecule that encodes the protein provided in FIG. 2, SEQ ID NO:2. A nucleic acid molecule consists essentially of a nucleotide sequence when such a nucleotide sequence is present with only a few additional nucleic acid residues in the final nucleic acid molecule.

The present invention further provides nucleic acid molecules that comprise the nucleotide sequences shown in FIG. 1 or 3 (SEQ ID NO:1, transcript sequence and SEQ ID NO:3, genomic sequence), or any nucleic acid molecule that encodes the protein provided in FIG. 2, SEQ ID NO:2. A nucleic acid molecule comprises a nucleotide sequence when the nucleotide sequence is at least part of the final nucleotide sequence of the nucleic acid molecule. In such a fashion, the nucleic acid molecule can be only the nucleotide sequence or have additional nucleic acid residues, such as nucleic acid residues that are naturally associated with it or heterologous nucleotide sequences. Such a nucleic acid molecule can have a few additional nucleotides or can comprises several hundred or more additional nucleotides. A brief description of how various types of these nucleic acid molecules can be readily made/isolated is provided below.

In FIGS. 1 and 3, both coding and non-coding sequences are provided. Because of the source of the present invention, humans genomic sequence (FIG. 3) and cDNA/transcript sequences (FIG. 1 ), the nucleic acid molecules in the Figures will contain genomic intronic sequences, 5′ and 3′ non-coding sequences, gene regulatory regions and non-coding intergenic sequences. In general such sequence features are either noted in FIGS. 1 and 3 or can readily be identified using computational tools known in the art. As discussed below, some of the non-coding regions, particularly gene regulatory elements such as promoters, are useful for a variety of purposes, e.g. control of heterologous gene expression, target for identifying gene activity modulating compounds, and are particularly claimed as fragments of the genomic sequence provided herein.

The isolated nucleic acid molecules can encode the mature protein plus additional amino or carboxyl-terminal amino acids, or amino acids interior to the mature peptide (when the mature form has more than one peptide chain, for instance). Such sequences may play a role in processing of a protein from precursor to a mature form, facilitate protein trafficking, prolong or shorten protein half-life or facilitate manipulation of a protein for assay or production, among other things. As generally is the case in situ, the additional amino acids may be processed away from the mature protein by cellular enzymes.

As mentioned above, the isolated nucleic acid molecules include, but are not limited to, the sequence encoding the phosphatase peptide alone, the sequence encoding the mature peptide and additional coding sequences, such as a leader or secretory sequence (e.g., a pre-pro or pro-protein sequence), the sequence encoding the mature peptide, with or without the additional coding sequences, plus additional non-coding sequences, for example introns and non-coding 5′ and 3′ sequences such as transcribed but non-translated sequences that play a role in transcription, mRNA processing (including splicing and polyadenylation signals), ribosome binding and stability of mRNA. In addition, the nucleic acid molecule may be fused to a marker sequence encoding, for example, a peptide that facilitates purification.

Isolated nucleic acid molecules can be in the form of RNA, such as mRNA, or in the form DNA, including cDNA and genomic DNA obtained by cloning or produced by chemical synthetic techniques or by a combination thereof. The nucleic acid, especially DNA, can be double-stranded or single-stranded. Single-stranded nucleic acid can be the coding strand (sense strand) or the non-coding strand (anti-sense strand).

The invention further provides nucleic acid molecules that encode fragments of the peptides of the present invention as well as nucleic acid molecules that encode obvious variants of the phosphatase proteins of the present invention that are described above. Such nucleic acid molecules may be naturally occurring, such as allelic variants (same locus), paralogs (different locus), and orthologs (different organism), or may be constructed by recombinant DNA methods or by chemical synthesis. Such non-naturally occurring variants may be made by mutagenesis techniques, including those applied to nucleic acid molecules, cells, or organisms. Accordingly, as discussed above, the variants can contain nucleotide substitutions, deletions, inversions and insertions. Variation can occur in either or both the coding and non-coding regions. The variations can produce both conservative and non-conservative amino acid substitutions.

The present invention further provides non-coding fragments of the nucleic acid molecules provided in FIGS. 1 and 3. Preferred non-coding fragments include, but are not limited to, promoter sequences, enhancer sequences, gene modulating sequences and gene termination sequences. Such fragments are useful in controlling heterologous gene expression and in developing screens to identify gene-modulating agents. A promoter can readily be identified as being 5′ to the ATG start site in the genomic sequence provided in FIG. 3 .

A fragment comprises a contiguous nucleotide sequence greater than 12 or more nucleotides. Further, a fragment could at least 30, 40, 50, 100, 250 or 500 nucleotides in length. The length of the fragment will be based on its intended use. For example, the fragment can encode epitope bearing regions of the peptide, or can be useful as DNA probes and primers. Such fragments can be isolated using the known nucleotide sequence to synthesize an oligonucleotide probe. A labeled probe can then be used to screen a cDNA library, genomic DNA library, or mRNA to isolate nucleic acid corresponding to the coding region. Further, primers can be used in PCR reactions to clone specific regions of gene.

A probe/primer typically comprises substantially a purified oligonucleotide or oligonucleotide pair. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, 20, 25, 40, 50 or more consecutive nucleotides.

Orthologs, homologs, and allelic variants can be identified using methods well known in the art. As described in the Peptide Section, these variants comprise a nucleotide sequence encoding a peptide that is typically 60-70%, 70-80%, 80-90%, and more typically at least about 90-95% or more homologous to the nucleotide sequence shown in the Figure sheets or a fragment of this sequence. Such nucleic acid molecules can readily be identified as being able to hybridize under moderate to stringent conditions, to the nucleotide sequence shown in the Figure sheets or a fragment of the sequence. Allelic variants can readily be determined by genetic locus of the encoding gene. As indicated by the data presented in FIG. 3, the map position was determined to be on chromosome 12 by ePCR.

FIG. 3 provides information on SNPs that have been identified in a gene encoding the phosphatase protein of the present invention. 82 SNP variants were found, including 6 indels (indicated by a “-”) and 3 SNPs in exons, of which 3 of these cause changes in the amino acid sequence (i.e., nonsynonymous SNPs). SNPs, identified at different nucleotide positions in introns and regions 5′ and 3′ of the ORF, may affect control/regulatory elements.

As used herein, the term “hybridizes under stringent conditions” is intended to describe conditions for hybridization and washing under which nucleotide sequences encoding a peptide at least 60-70% homologous to each other typically remain hybridized to each other. The conditions can be such that sequences at least about 60%, at least about 70%, or at least about 80% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those skilled in the art and can be found in Current Protocols in Molecular Biology , John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. One example of stringent hybridization conditions are hybridization in 6×sodium chloride/sodium citrate (SSC) at about 45C, followed by one or more washes in 0.2×SSC, 0.1% SDS at 50-65C. Examples of moderate to low stringency hybridization conditions are well known in the art.

Nucleic Acid Molecule Uses

The nucleic acid molecules of the present invention are useful for probes, primers, chemical intermediates, and in biological assays. The nucleic acid molecules are useful as a hybridization probe for messenger RNA, transcript/cDNA and genomic DNA to isolate full-length cDNA and genomic clones encoding the peptide described in FIG. 2 and to isolate cDNA and genomic clones that correspond to variants (alleles, orthologs, etc.) producing the same or related peptides shown in FIG. 2. 82 SNPs, including 6 indels, have been identified in the gene encoding the phosphatase protein provided by the present invention and are given in FIG. 3 .

The probe can correspond to any sequence along the entire length of the nucleic acid molecules provided in the Figures. Accordingly, it could be derived from 5′ noncoding regions, the coding region, and 3′ noncoding regions. However, as discussed, fragments are not to be construed as encompassing fragments disclosed prior to the present invention.

The nucleic acid molecules are also useful as primers for PCR to amplify any given region of a nucleic acid molecule and are useful to synthesize antisense molecules of desired length and sequence.

The nucleic acid molecules are also useful for constructing recombinant vectors. Such vectors include expression vectors that express a portion of, or all of, the peptide sequences. Vectors also include insertion vectors, used to integrate into another nucleic acid molecule sequence, such as into the cellular genome, to alter in situ expression of a gene and/or gene product. For example, an endogenous coding sequence can be replaced via homologous recombination with all or part of the coding region containing one or more specifically introduced mutations.

The nucleic acid molecules are also useful for expressing antigenic portions of the proteins.

The nucleic acid molecules are also useful as probes for determining the chromosomal positions of the nucleic acid molecules by means of in situ hybridization methods. As indicated by the data presented in FIG. 3, the map position was determined to be on chromosome 12 by ePCR.

The nucleic acid molecules are also useful in making vectors containing the gene regulatory regions of the nucleic acid molecules of the present invention.

The nucleic acid molecules are also useful for designing ribozymes corresponding to all, or a part, of the mRNA produced from the nucleic acid molecules described herein.

The nucleic acid molecules are also useful for making vectors that express part, or all, of the peptides.

The nucleic acid molecules are also useful for constructing host cells expressing a part, or all, of the nucleic acid molecules and peptides.

The nucleic acid molecules are also useful for constructing transgenic animals expressing all, or a part, of the nucleic acid molecules and peptides.

The nucleic acid molecules are also useful as hybridization probes for determining the presence, level, form and distribution of nucleic acid expression. Experimental data as provided in FIG. 1 indicates that phosphatase proteins of the present invention are expressed in the colon adenocarcinoma detected by a virtual northern blot. In addition, PCR-based tissue screening panel indicates expression in and placenta. Accordingly, the probes can be used to detect the presence of, or to determine levels of, a specific nucleic acid molecule in cells, tissues, and in organisms. The nucleic acid whose level is determined can be DNA or RNA. Accordingly, probes corresponding to the peptides described herein can be used to assess expression and/or gene copy number in a given cell, tissue, or organism. These uses are relevant for diagnosis of disorders involving an increase or decrease in phosphatase protein expression relative to normal results.

In vitro techniques for detection of mRNA include Northern hybridizations and in situ hybridizations. In vitro techniques for detecting DNA includes Southern hybridizations and in situ hybridization.

Probes can be used as a part of a diagnostic test kit for identifying cells or tissues that express a phosphatase protein, such as by measuring a level of a phosphatase-encoding nucleic acid in a sample of cells from a subject e.g., mRNA or genomic DNA, or determining if a phosphatase gene has been mutated. Experimental data as provided in FIG. 1 indicates that phosphatase proteins of the present invention are expressed in the colon adenocarcinoma detected by a virtual northern blot. In addition, PCR-based tissue screening panel indicates expression in and placenta.

Nucleic acid expression assays are useful for drug screening to identify compounds that modulate phosphatase nucleic acid expression.

The invention thus provides a method for identifying a compound that can be used to treat a disorder associated with nucleic acid expression of the phosphatase gene, particularly biological and pathological processes that are mediated by the phosphatase in cells and tissues that express it. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta. The method typically includes assaying the ability of the compound to modulate the expression of the phosphatase nucleic acid and thus identifying a compound that can be used to treat a disorder characterized by undesired phosphatase nucleic acid expression. The assays can be performed in cell-based and cell-free systems. Cell-based assays include cells naturally expressing the phosphatase nucleic acid or recombinant cells genetically engineered to express specific nucleic acid sequences.

The assay for phosphatase nucleic acid expression can involve direct assay of nucleic acid levels, such as mRNA levels, or on collateral compounds involved in the signal pathway. Further, the expression of genes that are up- or down-regulated in response to the phosphatase protein signal pathway can also be assayed. In this embodiment the regulatory regions of these genes can be operably linked to a reporter gene such as luciferase.

Thus, modulators of phosphatase gene expression can be identified in a method wherein a cell is contacted with a candidate compound and the expression of mRNA determined. The level of expression of phosphatase mRNA in the presence of the candidate compound is compared to the level of expression of phosphatase mRNA in the absence of the candidate compound. The candidate compound can then be identified as a modulator of nucleic acid expression based on this comparison and be used, for example to treat a disorder characterized by aberrant nucleic acid expression. When expression of mRNA is statistically significantly greater in the presence of the candidate compound than in its absence, the candidate compound is identified as a stimulator of nucleic acid expression. When nucleic acid expression is statistically significantly less in the presence of the candidate compound than in its absence, the candidate compound is identified as an inhibitor of nucleic acid expression.

The invention further provides methods of treatment, with the nucleic acid as a target, using a compound identified through drug screening as a gene modulator to modulate phosphatase nucleic acid expression in cells and tissues that express the phosphatase. Experimental data as provided in FIG. 1 indicates that phosphatase proteins of the present invention are expressed in the colon adenocarcinoma detected by a virtual northern blot. In addition, PCR-based tissue screening panel indicates expression in and placenta. Modulation includes both up-regulation (i.e. activation or agonization) or down-regulation (suppression or antagonization) or nucleic acid expression.

Alternatively, a modulator for phosphatase nucleic acid expression can be a small molecule or drug identified using the screening assays described herein as long as the drug or small molecule inhibits the phosphatase nucleic acid expression in the cells and tissues that express the protein. Experimental data as provided in FIG. 1 indicates expression in colon adenocarcinoma and placenta.

The nucleic acid molecules are also useful for monitoring the effectiveness of modulating compounds on the expression or activity of the phosphatase gene in clinical trials or in a treatment regimen. Thus, the gene expression pattern can serve as a barometer for the continuing effectiveness of treatment with the compound, particularly with compounds to which a patient can develop resistance. The gene expression pattern can also serve as a marker indicative of a physiological response of the affected cells to the compound. Accordingly, such monitoring would allow either increased administration of the compound or the administration of alternative compounds to which the patient has not become resistant. Similarly, if the level of nucleic acid expression falls below a desirable level, administration of the compound could be commensurately decreased.

The nucleic acid molecules are also useful in diagnostic assays for qualitative changes in phosphatase nucleic acid expression, and particularly in qualitative changes that lead to pathology. The nucleic acid molecules can be used to detect mutations in phosphatase genes and gene expression products such as mRNA. The nucleic acid molecules can be used as hybridization probes to detect naturally occurring genetic mutations in the phosphatase gene and thereby to determine whether a subject with the mutation is at risk for a disorder caused by the mutation. Mutations include deletion, addition, or substitution of one or more nucleotides in the gene, chromosomal rearrangement, such as inversion or transposition, modification of genomic DNA, such as aberrant methylation patterns or changes in gene copy number, such as amplification. Detection of a mutated form of the phosphatase gene associated with a dysfunction provides a diagnostic tool for an active disease or susceptibility to disease when the disease results from overexpression, underexpression, or altered expression of a phosphatase protein.

Individuals carrying mutations in the phosphatase gene can be detected at the nucleic acid level by a variety of techniques. FIG. 3 provides information on SNPs that have been identified in a gene encoding the phosphatase protein of the present invention. 82 SNP variants were found, including 6 indels (indicated by a “-”) and 3 SNPs in exons, of which 3 of these cause changes in the amino acid sequence (i.e., nonsynonymous SNPs). SNPs, identified at different nucleotide positions in introns and regions 5′ and 3′ of the ORF, may affect control/regulatory elements. As indicated by the data presented in FIG. 3, the map position was determined to be on chromosome 12 by ePCR. Genomic DNA can be analyzed directly or can be amplified by using PCR prior to analysis. RNA or cDNA can be used in the same way. In some uses, detection of the mutation involves the use of a probe/primer in a polymerase chain reaction (PCR) (see, e.g. U.S. Pat. Nos. 4,683,195 and 4,683,202), such as anchor PCR or RACE PCR, or, alternatively, in a ligation chain reaction (LCR) (see, e.g., Landegran et al., Science 241:1077-1080 (1988); and Nakazawa et al., PNAS 91:360-364 (1994)), the latter of which can be particularly useful for detecting point mutations in the gene (see Abravaya et al., Nucleic Acids Res. 23:675-682 (1995)). This method can include the steps of collecting a sample of cells from a patient, isolating nucleic acid (e.g., genomic, mRNA or both) from the cells of the sample, contacting the nucleic acid sample with one or more primers which specifically hybridize to a gene under conditions such that hybridization and amplification of the gene (if present) occurs, and detecting the presence or absence of an amplification product, or detecting the size of the amplification product and comparing the length to a control sample. Deletions and insertions can be detected by a change in size of the amplified product compared to the normal genotype. Point mutations can be identified by hybridizing amplified DNA to normal RNA or antisense DNA sequences.

Alternatively, mutations in a phosphatase gene can be directly identified, for example, by alterations in restriction enzyme digestion patterns determined by gel electrophoresis.

Further, sequence-specific ribozymes (U.S. Pat. No. 5,498,531) can be used to score for the presence of specific mutations by development or loss of a ribozyme cleavage site. Perfectly matched sequences can be distinguished from mismatched sequences by nuclease cleavage digestion assays or by differences in melting temperature.

Sequence changes at specific locations can also be assessed by nuclease protection assays such as RNase and S1 protection or the chemical cleavage method. Furthermore, sequence differences between a mutant phosphatase gene and a wild-type gene can be determined by direct DNA sequencing. A variety of automated sequencing procedures can be utilized when performing the diagnostic assays (Naeve, C. W., (1995) Biotechniques 19:448), including sequencing by mass spectrometry (see, e.g., PCT International Publication No. WO94/16101; Cohen et al, Adv. Chromatogr. 36:127-162 (1996); and Griffin et al., Appl. Biochem. Biotechnol. 38:147-159 (1993)).

Other methods for detecting mutations in the gene include methods in which protection from cleavage agents is used to detect mismatched bases in RNA/RNA or RNA/DNA duplexes (Myers et al., Science 230:1242 (1985)); Cotton et al., PNAS 85:4397 (1988); Saleeba et al., Meth. Enzymol. 217:286-295 (1992)), electrophoretic mobility of mutant and wild type nucleic acid is compared (Orita et al., PNAS 86:2766 (1989); Cotton et al., Mutat. Res. 285:125-144 (1993); and Hayashi et al., Genet. Anal. Tech. Appl. 9:73-79 (1992)), and movement of mutant or wild-type fragments in polyacrylamide gels containing a gradient of denaturant is assayed using denaturing gradient gel electrophoresis (Myers et al., Nature 313:495 (1985)). Examples of other techniques for detecting point mutations include selective oligonucleotide hybridization, selective amplification, and selective primer extension.

The nucleic acid molecules are also useful for testing an individual for a genotype that while not necessarily causing the disease, nevertheless affects the treatment modality. Thus, the nucleic acid molecules can be used to study the relationship between an individual's genotype and the individual's response to a compound used for treatment (pharmacogenomic relationship). Accordingly, the nucleic acid molecules described herein can be used to assess the mutation content of the phosphatase gene in an individual in order to select an appropriate compound or dosage regimen for treatment. FIG. 3 provides information on SNPs that have been identified in a gene encoding the phosphatase protein of the present invention. 82 SNP variants were found, including 6 indels (indicated by a “-”) and 3 SNPs in exons, of which 3 of these cause changes in the amino acid sequence (i.e., nonsynonymous SNPs). SNPs, identified at different nucleotide positions in introns and regions 5′ and 3′ of the ORF, may affect control/regulatory elements.

Thus nucleic acid molecules displaying genetic variations that affect treatment provide a diagnostic target that can be used to tailor treatment in an individual. Accordingly, the production of recombinant cells and animals containing these polymorphisms allow effective clinical design of treatment compounds and dosage regimens.

The nucleic acid molecules are thus useful as antisense constructs to control phosphatase gene expression in cells, tissues, and organisms. A DNA antisense nucleic acid molecule is designed to be complementary to a region of the gene involved in transcription, preventing transcription and hence production of phosphatase protein. An antisense RNA or DNA nucleic acid molecule would hybridize to the mRNA and thus block translation of mRNA into phosphatase protein.

Alternatively, a class of antisense molecules can be used to inactivate mRNA in order to decrease expression of phosphatase nucleic acid. Accordingly, these molecules can treat a disorder characterized by abnormal or undesired phosphatase nucleic acid expression. This technique involves cleavage by means of ribozymes containing nucleotide sequences complementary to one or more regions in the mRNA that attenuate the ability of the mRNA to be translated. Possible regions include coding regions and particularly coding regions corresponding to the catalytic and other functional activities of the phosphatase protein, such as substrate binding.

The nucleic acid molecules also provide vectors for gene therapy in patients containing cells that are aberrant in phosphatase gene expression. Thus, recombinant cells, which include the patient's cells that have been engineered ex vivo and returned to the patient, are introduced into an individual where the cells produce the desired phosphatase protein to treat the individual.

The invention also encompasses kits for detecting the presence of a phosphatase nucleic acid in a biological sample. Experimental data as provided in FIG. 1 indicates that phosphatase proteins of the present invention are expressed in the colon adenocarcinoma detected by a virtual northern blot. In addition, PCR-based tissue screening panel indicates expression in and placenta. For example, the kit can comprise reagents such as a labeled or labelable nucleic acid or agent capable of detecting phosphatase nucleic acid in a biological sample; means for determining the amount of phosphatase nucleic acid in the sample; and means for comparing the amount of phosphatase nucleic acid in the sample with a standard. The compound or agent can be packaged in a suitable container. The kit can further comprise instructions for using the kit to detect phosphatase protein mRNA or DNA.

Nucleic Acid Arrays

The present invention further provides nucleic acid detection kits, such as arrays or microarrays of nucleic acid molecules that are based on the sequence information provided in FIGS. 1 and 3 (SEQ ID NOS:1 and 3).

As used herein “Arrays” or “Microarrays” refers to an array of distinct polynucleotides or oligonucleotides synthesized on a substrate, such as paper, nylon or other type of membrane, filter, chip, glass slide, or any other suitable solid support. In one embodiment, the microarray is prepared and used according to the methods described in U.S. Pat. No. 5,837,832, Chee et al., PCT application WO95/11995 (Chee et al.), Lockhart, D. J. et al. (1996; Nat. Biotech. 14: 1675-1680) and Schena, M. et al. (1996; Proc. Natl. Acad. Sci. 93: 10614-10619), all of which are incorporated herein in their entirety by reference. In other embodiments, such arrays are produced by the methods described by Brown et al., U.S. Pat. No. 5,807,522.

The microarray or detection kit is preferably composed of a large number of unique, single-stranded nucleic acid sequences, usually either synthetic antisense oligonucleotides or fragments of cDNAs, fixed to a solid support. The oligonucleotides are preferably about 6-60 nucleotides in length, more preferably 15-30 nucleotides in length, and most preferably about 20-25 nucleotides in length. For a certain type of microarray or detection kit, it may be preferable to use oligonucleotides that are only 7-20 nucleotides in length. The microarray or detection kit may contain oligonucleotides that cover the known 5′, or 3′, sequence, sequential oligonucleotides which cover the full length sequence; or unique oligonucleotides selected from particular areas along the length of the sequence. Polynucleotides used in the microarray or detection kit may be oligonucleotides that are specific to a gene or genes of interest.

In order to produce oligonucleotides to a known sequence for a microarray or detection kit, the gene(s) of interest (or an ORF identified from the contigs of the present invention) is typically examined using a computer algorithm which starts at the 5′ or at the 3′ end of the nucleotide sequence. Typical algorithms will then identify oligomers of defined length that are unique to the gene, have a GC content within a range suitable for hybridization, and lack predicted secondary structure that may interfere with hybridization. In certain situations it may be appropriate to use pairs of oligonucleotides on a microarray or detection kit. The “pairs” will be identical, except for one nucleotide that preferably is located in the center of the sequence. The second oligonucleotide in the pair (mismatched by one) serves as a control. The number of oligonucleotide pairs may range from two to one million. The oligomers are synthesized at designated areas on a substrate using a light-directed chemical process. The substrate may be paper, nylon or other type of membrane, filter, chip, glass slide or any other suitable solid support.

In another aspect, an oligonucleotide may be synthesized on the surface of the substrate by using a chemical coupling procedure and an ink jet application apparatus, as described in PCT application WO95/251116 (Baldeschweiler et al.) which is incorporated herein in its entirety by reference. In another aspect, a “gridded” array analogous to a dot (or slot) blot may be used to arrange and link cDNA fragments or oligonucleotides to the surface of a substrate using a vacuum system, thermal, UV, mechanical or chemical bonding procedures. An array, such as those described above, may be produced by hand or by using available devices (slot blot or dot blot apparatus), materials (any suitable solid support), and machines (including robotic instruments), and may contain 8, 24, 96, 384, 1536, 6144 or more oligonucleotides, or any other number between two and one million which lends itself to the efficient use of commercially available instrumentation.

In order to conduct sample analysis using a microarray or detection kit, the RNA or DNA from a biological sample is made into hybridization probes. The mRNA is isolated, and cDNA is produced and used as a template to make antisense RNA (aRNA). The aRNA is amplified in the presence of fluorescent nucleotides, and labeled probes are incubated with the microarray or detection kit so that the probe sequences hybridize to complementary oligonucleotides of the microarray or detection kit. Incubation conditions are adjusted so that hybridization occurs with precise complementary matches or with various degrees of less complementarity. After removal of nonhybridized probes, a scanner is used to determine the levels and patterns of fluorescence. The scanned images are examined to determine degree of complementarity and the relative abundance of each oligonucleotide sequence on the microarray or detection kit. The biological samples may be obtained from any bodily fluids (such as blood, urine, saliva, phlegm, gastric juices, etc.), cultured cells, biopsies, or other tissue preparations. A detection system may be used to measure the absence, presence, and amount of hybridization for all of the distinct sequences simultaneously. This data may be used for large-scale correlation studies on the sequences, expression patterns, mutations, variants, or polymorphisms among samples.

Using such arrays, the present invention provides methods to identify the expression of the phosphatase proteins/peptides of the present invention. In detail, such methods comprise incubating a test sample with one or more nucleic acid molecules and assaying for binding of the nucleic acid molecule with components within the test sample. Such assays will typically involve arrays comprising many genes, at least one of which is a gene of the present invention and or alleles of the phosphatase gene of the present invention. FIG. 3 provides information on SNPs that have been identified in a gene encoding the phosphatase protein of the present invention. 82 SNP variants were found, including 6 indels (indicated by a “-”) and 3 SNPs in exons, of which 3 of these cause changes in the amino acid sequence (i.e., nonsynonymous SNPs). SNPs, identified at different nucleotide positions in introns and regions 5′ and 3′ of the ORF, may affect control/regulatory elements.

Conditions for incubating a nucleic acid molecule with a test sample vary. Incubation conditions depend on the format employed in the assay, the detection methods employed, and the type and nature of the nucleic acid molecule used in the assay. One skilled in the art will recognize that any one of the commonly available hybridization, amplification or array assay formats can readily be adapted to employ the novel fragments of the Human genome disclosed herein. Examples of such assays can be found in Chard, T, An Introduction to Radioimmunoassay and Related Techniques , Elsevier Science Publishers, Amsterdam, The Netherlands (1986); Bullock, G. R. et al., Techniques in Immunocytochemistry , Academic Press, Orlando, Fla. Vol. 1 (1 982), Vol. 2 (1983), Vol. 3 (1985); Tijssen, P., Practice and Theory of Enzyme Immunoassays: Laboratory Techniques in Biochemistry and Molecular Biology , Elsevier Science Publishers, Amsterdam, The Netherlands (1985).

The test samples of the present invention include cells, protein or membrane extracts of cells. The test sample used in the above-described method will vary based on the assay format, nature of the detection method and the tissues, cells or extracts used as the sample to be assayed. Methods for preparing nucleic acid extracts or of cells are well known in the art and can be readily be adapted in order to obtain a sample that is compatible with the system utilized.

In another embodiment of the present invention, kits are provided which contain the necessary reagents to carry out the assays of the present invention.

Specifically, the invention provides a compartmentalized kit to receive, in close confinement, one or more containers which comprises: (a) a first container comprising one of the nucleic acid molecules that can bind to a fragment of the Human genome disclosed herein; and (b) one or more other containers comprising one or more of the following: wash reagents, reagents capable of detecting presence of a bound nucleic acid.

In detail, a compartmentalized kit includes any kit in which reagents are contained in separate containers. Such containers include small glass containers, plastic containers, strips of plastic, glass or paper, or arraying material such as silica. Such containers allows one to efficiently transfer reagents from one compartment to another compartment such that the samples and reagents are not cross-contaminated, and the agents or solutions of each container can be added in a quantitative fashion from one compartment to another. Such containers will include a container which will accept the test sample, a container which contains the nucleic acid probe, containers which contain wash reagents (such as phosphate buffered saline, Tris-buffers, etc.), and containers which contain the reagents used to detect the bound probe. One skilled in the art will readily recognize that the previously unidentified phosphatase gene of the present invention can be routinely identified using the sequence information disclosed herein can be readily incorporated into one of the established kit formats which are well known in the art, particularly expression arrays.

Vectors/Host Cells

The invention also provides vectors containing the nucleic acid molecules described herein. The term “vector” refers to a vehicle, preferably a nucleic acid molecule, which can transport the nucleic acid molecules. When the vector is a nucleic acid molecule, the nucleic acid molecules are covalently linked to the vector nucleic acid. With this aspect of the invention, the vector includes a plasmid, single or double stranded phage, a single or double stranded RNA or DNA viral vector, or artificial chromosome, such as a BAC, PAC, YAC, OR MAC.

A vector can be maintained in the host cell as an extrachromosomal element where it replicates and produces additional copies of the nucleic acid molecules. Alternatively, the vector may integrate into the host cell genome and produce additional copies of the nucleic acid molecules when the host cell replicates.

The invention provides vectors for the maintenance (cloning vectors) or vectors for expression (expression vectors) of the nucleic acid molecules. The vectors can function in prokaryotic or eukaryotic cells or in both (shuttle vectors).

Expression vectors contain cis-acting regulatory regions that are operably linked in the vector to the nucleic acid molecules such that transcription of the nucleic acid molecules is allowed in a host cell. The nucleic acid molecules can be introduced into the host cell with a separate nucleic acid molecule capable of affecting transcription. Thus, the second nucleic acid molecule may provide a trans-acting factor interacting with the cis-regulatory control region to allow transcription of the nucleic acid molecules from the vector. Alternatively, a trans-acting factor may be supplied by the host cell. Finally, a trans-acting factor can be produced from the vector itself. It is understood, however, that in some embodiments, transcription and/or translation of the nucleic acid molecules can occur in a cell-free system.

The regulatory sequence to which the nucleic acid molecules described herein can be operably linked include promoters for directing mRNA transcription. These include, but are not limited to, the left promoter from bacteriophage λ, the lac, TRP, and TAC promoters from E. coli , the early and late promoters from SV40, the CMV immediate early promoter, the adenovirus early and late promoters, and retrovirus long-terminal repeats.

In addition to control regions that promote transcription, expression vectors may also include regions that modulate transcription, such as repressor binding sites and enhancers. Examples include the SV40 enhancer, the cytomegalovirus immediate early enhancer, polyoma enhancer, adenovirus enhancers, and retrovirus LTR enhancers.

In addition to containing sites for transcription initiation and control, expression vectors can also contain sequences necessary for transcription termination and, in the transcribed region a ribosome binding site for translation. Other regulatory control elements for expression include initiation and termination codons as well as polyadenylation signals. The person of ordinary skill in the art would be aware of the numerous regulatory sequences that are useful in expression vectors. Such regulatory sequences are described, for example, in Sambrook et al., Molecular Cloning: A Laboratory Manual. 2 nd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., (1989).

A variety of expression vectors can be used to express a nucleic acid molecule. Such vectors include chromosomal, episomal, and virus-derived vectors, for example vectors derived from bacterial plasmids, from bacteriophage, from yeast episomes, from yeast chromosomal elements, including yeast artificial chromosomes, from viruses such as baculoviruses, papovaviruses such as SV40, Vaccinia viruses, adenoviruses, poxviruses, pseudorabies viruses, and retroviruses. Vectors may also be derived from combinations of these sources such as those derived from plasmid and bacteriophage genetic elements, e.g. cosmids and phagemids. Appropriate cloning and expression vectors for prokaryotic and eukaryotic hosts are described in Sambrook et al., Molecular Cloning: A Laboratory Manual. 2 nd. ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., (1989).

The regulatory sequence may provide constitutive expression in one or more host cells (i.e. tissue specific) or may provide for inducible expression in one or more cell types such as by temperature, nutrient additive, or exogenous factor such as a hormone or other ligand. A variety of vectors providing for constitutive and inducible expression in prokaryotic and eukaryotic hosts are well known to those of ordinary skill in the art.

The nucleic acid molecules can be inserted into the vector nucleic acid by well-known methodology. Generally, the DNA sequence that will ultimately be expressed is joined to an expression vector by cleaving the DNA sequence and the expression vector with one or more restriction enzymes and then ligating the fragments together. Procedures for restriction enzyme digestion and ligation are well known to those of ordinary skill in the art.

The vector containing the appropriate nucleic acid molecule can be introduced into an appropriate host cell for propagation or expression using well-known techniques. Bacterial cells include, but are not limited to, E. coli , Streptomyces, and Salmonella typhimurium . Eukaryotic cells include, but are not limited to, yeast, insect cells such as Drosophila, animal cells such as COS and CHO cells, and plant cells.

As described herein, it may be desirable to express the peptide as a fusion protein. Accordingly, the invention provides fusion vectors that allow for the production of the peptides. Fusion vectors can increase the expression of a recombinant protein, increase the solubility of the recombinant protein, and aid in the purification of the protein by acting for example as a ligand for affinity purification. A proteolytic cleavage site may be introduced at the junction of the fusion moiety so that the desired peptide can ultimately be separated from the fusion moiety. Proteolytic enzymes include, but are not limited to, factor Xa, thrombin, and enterophosphatase. Typical fusion expression vectors include pGEX (Smith et al., Gene 67:31-40 (1988)), pMAL (New England Biolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amann et al., Gene 69:301-315 (1988)) and pET 11d (Studier et al., Gene Expression Technology: Methods in Enzymology 185:60-89 (1990)).

Recombinant protein expression can be maximized in host bacteria by providing a genetic background wherein the host cell has an impaired capacity to proteolytically cleave the recombinant protein. (Gottesman, S., Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, Calif. (1990)119-128). Alternatively, the sequence of the nucleic acid molecule of interest can be altered to provide preferential codon usage for a specific host cell, for example E. coli . (Wada et al., Nucleic Acids Res. 20:2111-2118 (1992)).

The nucleic acid molecules can also be expressed by expression vectors that are operative in yeast. Examples of vectors for expression in yeast e.g., S. cerevisiae include pYepSec1 (Baldari, et al., EMBO J. 6:229-234 (1987)), pMFa (Kurjan et al., Cell 30:933-943(1982) pJRY88 (Schultz et al., Gene 54:113-123 (1987)), and pYES2 (Invitrogen Corporation, San Diego, Calif.).

The nucleic acid molecules can also be expressed in insect cells using, for example, baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells (e.g., Sf9 cells) include the pAc series (Smith et al., Mol. Cell Biol. 3:2156-2165 (1983)) and the pVL series (Lucklow et al., Virology 170:31-39 (1989)).

In certain embodiments of the invention, the nucleic acid molecules described herein are expressed in mammalian cells using mammalian expression vectors. Examples of mammalian expression vectors include pCDM8 (Seed, B. Nature 329:840(1987)) and pMT2PC (Kaufman et al., EMBO J 6:187-195 (1987)).

The expression vectors listed herein are provided by way of example only of the well-known vectors available to those of ordinary skill in the art that would be useful to express the nucleic acid molecules. The person of ordinary skill in the art would be aware of other vectors suitable for maintenance propagation or expression of the nucleic acid molecules described herein. These are found for example in Sambrook, J., Fritsh, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2 nd, ed., Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989.

The invention also encompasses vectors in which the nucleic acid sequences described herein are cloned into the vector in reverse orientation, but operably linked to a regulatory sequence that permits transcription of antisense RNA. Thus, an antisense transcript can be produced to all, or to a portion, of the nucleic acid molecule sequences described herein, including both coding and non-coding regions. Expression of this antisense RNA is subject to each of the parameters described above in relation to expression of the sense RNA (regulatory sequences, constitutive or inducible expression, tissue-specific expression).

The invention also relates to recombinant host cells containing the vectors described herein. Host cells therefore include prokaryotic cells, lower eukaryotic cells such as yeast, other eukaryotic cells such as insect cells, and higher eukaryotic cells such as mammalian cells.

The recombinant host cells are prepared by introducing the vector constructs described herein into the cells by techniques readily available to the person of ordinary skill in the art. These include, but are not limited to, calcium phosphate transfection, DEAE-dextran-mediated transfection, cationic lipid-mediated transfection, electroporation, transduction, infection, lipofection, and other techniques such as those found in Sambrook, et al. ( Molecular Cloning: A Laboratory Manual. 2 nd, ed., Cold Spring Harbor Laboratory , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1989).

Host cells can contain more than one vector. Thus, different nucleotide sequences can be introduced on different vectors of the same cell. Similarly, the nucleic acid molecules can be introduced either alone or with other nucleic acid molecules that are not related to the nucleic acid molecules such as those providing trans-acting factors for expression vectors. When more than one vector is introduced into a cell, the vectors can be introduced independently, co-introduced or joined to the nucleic acid molecule vector.

In the case of bacteriophage and viral vectors, these can be introduced into cells as packaged or encapsulated virus by standard procedures for infection and transduction. Viral vectors can be replication-competent or replication-defective. In the case in which viral replication is defective, replication will occur in host cells providing functions that complement the defects.

Vectors generally include selectable markers that enable the selection of the subpopulation of cells that contain the recombinant vector constructs. The marker can be contained in the same vector that contains the nucleic acid molecules described herein or may be on a separate vector. Markers include tetracycline or ampicillin-resistance genes for prokaryotic host cells and dihydrofolate reductase or neomycin resistance for eukaryotic host cells. However, any marker that provides selection for a phenotypic trait will be effective.

While the mature proteins can be produced in bacteria, yeast, mammalian cells, and other cells under the control of the appropriate regulatory sequences, cell-free transcription and translation systems can also be used to produce these proteins using RNA derived from the DNA constructs described herein.

Where secretion of the peptide is desired, which is difficult to achieve with multi-transmembrane domain containing proteins such as phosphatases, appropriate secretion signals are incorporated into the vector. The signal sequence can be endogenous to the peptides or heterologous to these peptides.

Where the peptide is not secreted into the medium, which is typically the case with phosphatases, the protein can be isolated from the host cell by standard disruption procedures, including freeze thaw, sonication, mechanical disruption, use of lysing agents and the like. The peptide can then be recovered and purified by well-known purification methods including ammonium sulfate precipitation, acid extraction, anion or cationic exchange chromatography, phosphocellulose chromatography, hydrophobic-interaction chromatography, affinity chromatography, hydroxylapatite chromatography, lectin chromatography, or high performance liquid chromatography.

It is also understood that depending upon the host cell in recombinant production of the peptides described herein, the peptides can have various glycosylation patterns, depending upon the cell, or maybe non-glycosylated as when produced in bacteria. In addition, the peptides may include an initial modified methionine in some cases as a result of a host-mediated process.

Uses of Vectors and Host Cells

The recombinant host cells expressing the peptides described herein have a variety of uses. First, the cells are useful for producing a phosphatase protein or peptide that can be further purified to produce desired amounts of phosphatase protein or fragments. Thus, host cells containing expression vectors are useful for peptide production.

Host cells are also useful for conducting cell-based assays involving the phosphatase protein or phosphatase protein fragments, such as those described above as well as other formats known in the art. Thus, a recombinant host cell expressing a native phosphatase protein is useful for assaying compounds that stimulate or inhibit phosphatase protein function.

Host cells are also useful for identifying phosphatase protein mutants in which these functions are affected. If the mutants naturally occur and give rise to a pathology, host cells containing the mutations are useful to assay compounds that have a desired effect on the mutant phosphatase protein (for example, stimulating or inhibiting function) which may not be indicated by their effect on the native phosphatase protein.

Genetically engineered host cells can be further used to produce non-human transgenic animals. A transgenic animal is preferably a mammal, for example a rodent, such as a rat or mouse, in which one or more of the cells of the animal include a transgene. A transgene is exogenous DNA which is integrated into the genome of a cell from which a transgenic animal develops and which remains in the genome of the mature animal in one or more cell types or tissues of the transgenic animal. These animals are useful for studying the function of a phosphatase protein and identifying and evaluating modulators of phosphatase protein activity. Other examples of transgenic animals include non-human primates, sheep, dogs, cows, goats, chickens, and amphibians.

A transgenic animal can be produced by introducing nucleic acid into the male pronuclei of a fertilized oocyte, e.g., by microinjection, retroviral infection, and allowing the oocyte to develop in a pseudopregnant female foster animal. Any of the phosphatase protein nucleotide sequences can be introduced as a transgene into the genome of a non-human animal, such as a mouse.

Any of the regulatory or other sequences useful in expression vectors can form part of the transgenic sequence. This includes intronic sequences and polyadenylation signals, if not already included. A tissue-specific regulatory sequence(s) can be operably linked to the transgene to direct expression of the phosphatase protein to particular cells.

Methods for generating transgenic animals via embryo manipulation and microinjection, particularly animals such as mice, have become conventional in the art and are described, for example, in U.S. Pat. Nos. 4,736,866 and 4,870,009, both by Leder et al., U.S. Pat. No. 4,873,191 by Wagner et al. and in Hogan, B., Manipulating the Mouse Embryo , (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986). Similar methods are used for production of other transgenic animals. A transgenic founder animal can be identified based upon the presence of the transgene in its genome and/or expression of transgenic mRNA in tissues or cells of the animals. A transgenic founder animal can then be used to breed additional animals carrying the transgene. Moreover, transgenic animals carrying a transgene can further be bred to other transgenic animals carrying other transgenes. A transgenic animal also includes animals in which the entire animal or tissues in the animal have been produced using the homologously recombinant host cells described herein.

In another embodiment, transgenic non-human animals can be produced which contain selected systems that allow for regulated expression of the transgene. One example of such a system is the cre/loxP recombinase system of bacteriophage P1. For a description of the cre/loxP recombinase system, see, e.g., Lakso et al. PNAS 89:6232-6236 (1992). Another example of a recombinase system is the FLP recombinase system of S. cerevisiae (O'Gorman et al. Science 251:1351-1355 (1991). If a cre/loxP recombinase system is used to regulate expression of the transgene, animals containing transgenes encoding both the Cre recombinase and a selected protein is required. Such animals can be provided through the construction of “double” transgenic animals, e.g., by mating two transgenic animals, one containing a transgene encoding a selected protein and the other containing a transgene encoding a recombinase.

Clones of the non-human transgenic animals described herein can also be produced according to the methods described in Wilmut, I. et al. Nature 385:810-813 (1997) and PCT International Publication Nos. WO 97/07668 and WO 97/07669. In brief, a cell, e.g., a somatic cell, from the transgenic animal can be isolated and induced to exit the growth cycle and enter G o phase. The quiescent cell can then be fused, e.g., through the use of electrical pulses, to an enucleated oocyte from an animal of the same species from which the quiescent cell is isolated. The reconstructed oocyte is then cultured such that it develops to morula or blastocyst and then transferred to pseudopregnant female foster animal. The offspring born of this female foster animal will be a clone of the animal from which the cell, e.g., the somatic cell, is isolated.

Transgenic animals containing recombinant cells that express the peptides described herein are useful to conduct the assays described herein in an in vivo context. Accordingly, the various physiological factors that are present in vivo and that could effect substrate binding, kinase protein activation, and signal transduction, may not be evident from in vitro cell-free or cell-based assays. Accordingly, it is useful to provide non-human transgenic animals to assay in vivo phosphatase protein function, including substrate interaction, the effect of specific mutant phosphatase proteins on phosphatase protein function and substrate interaction, and the effect of chimeric phosphatase proteins. It is also possible to assess the effect of null mutations, that is mutations that substantially or completely eliminate one or more phosphatase protein functions.

All publications and patents mentioned in the above specification are herein incorporated by reference. Various modifications and variations of the described method and system of the invention will be apparent to those skilled in the art without departing from the scope and spirit of the invention. Although the invention has been described in connection with specific preferred embodiments, it should be understood that the invention as claimed should not be unduly limited to such specific embodiments. Indeed, various modifications of the above-described modes for carrying out the invention which are obvious to those skilled in the field of molecular biology or related fields are intended to be within the scope of the following claims.

<160> NUMBER OF SEQ ID NOS: 4

<210> SEQ ID NO 1

<211> LENGTH: 7108

<212> TYPE: DNA

<213> ORGANISM: Human

<400> SEQUENCE: 1

taattgtgta cttgccagaa ggatctgtct ttaaatcatt aatgcaggca ac

#atttctct     60

ctagagccat caatgtgatt ctactggctg aaaaatgtaa taaagatgga tt

#ttcttatc    120

atttttcttt tactttttat tgggacttca gagacacagg ttgatgtttc ca

#atgtcgtt    180

cctggtacta ggtacgatat aaccatctct tcaatttcta caacatacac ct

#cacctgtt    240

actagaatag tgacaccaaa tgtaacaaaa ccagggcctc cagtcttcct ag

#ccggggaa    300

agagtcggat ctgctgggat tcttctgtct tggaatacac cacctaatcc aa

#atggaagg    360

attatatctt acattgtcaa atataaggaa gtttgtccgt ggatgcaaac ag

#tatataca    420

caagtcagat caaagccaga cagtctggaa gttcttctta ctaatcttaa tc

#ctggaaca    480

acatatgaaa ttaaggttgc tgctgaaaac agtgctggca ttggagtgtt ta

#gtgatcca    540

tttctcttcc aaactgcaga aagtgctcca ggaaaagtgg tgaatctcac ag

#ttgaggcc    600

tacaacgctt cagcagttaa gctgatttgg tatttacctc ggcaaccaaa tg

#gcaaaatt    660

accagcttca agattagtgt caaacatgcc agaagtggga tagtagtgaa ag

#atgtctca    720

atcagagtag aggacatttt gactgggaaa ttgccagaat gcaatgagaa ta

#gtgaatct    780

tttttatgga gtacagccag cccttctcca acccttggta gagttacacc tc

#catcgcgt    840

accacacatt catcaagcac gttgacacag aatgagatca gctctgtgtg ga

#aagagcct    900

atcagttttg tagtgacaca cttgagacct tatacaacat atctttttga ag

#tttcagct    960

gctacaactg aagcaggtta tattgatagt acgattgtca gaacaccaga at

#cagtgcct   1020

gaaggaccac cacaaaactg cgtaacaggc aacatcacag gaaagtcctt tt

#caatttta   1080

tgggacccac caactatagt aacagggaaa tttagttata gagttgaatt at

#atggacca   1140

tcaggtcgca ttttggataa cagcacaaaa gacctcaagt ttgcattcac ta

#acctaaca   1200

ccatttacaa tgtatgatgt ctatattgcg gctgaaacca gtgcagggac tg

#ggcccaag   1260

tcaaatattt cagtattcac tccaccagat gttccagggg cagtgtttga tt

#tacaactt   1320

gcagaggtag aatccacgca agtaagaatt acttggaaga aaccacgaca ac

#caaatgga   1380

attattaacc aataccgagt gaaagtgcta gttccagaga caggaataat tt

#tggaaaat   1440

actttgctca ctggaaataa tgagtatata aatgacccca tggctccaga aa

#ttgtgaac   1500

atagtagagc caatggtagg attatatgag ggttcagcag agatgtcgtc tg

#accttcac   1560

tcacttgcta catttatata taacagccat ccagataaaa actttcctgc aa

#ggaataga   1620

gctgaagacc agacttcacc agttgtaact acaaggaatc agtatattac tg

#acattgca   1680

gctgaacagc tgtcttatgt tatcaggaga cttgtacctt tcactgagca ca

#tgattagt   1740

gtatctgctt tcaccatcat gggagaagga ccaccaacag ttctcagtgt ta

#ggacacgt   1800

cagcaagtgc caagctccat taaaattata aactataaaa atattagttc tt

#catctatt   1860

ttgttatatt gggatcctcc agaatatccc aatggaaaaa taactcacta ta

#cgatttat   1920

gcaatggaat tggatacaaa cagagcattc cagataacta ccatagataa ca

#gctttctc   1980

ataacagggt taaagaaata cacaaaatac aaaatgagag tggcagcctc aa

#cccacgat   2040

ggagaaagtt ctttgtctga agaaaatgac atctttgtga gaacttcaga ag

#atgaaccg   2100

gaatcatcac ctcaagatgt cgaagtaatt gatgttaccg cagatgaaat aa

#ggttgaag   2160

tggtcaccac ccgaaaagcc caatgggatc attattgctt atgaagtgct at

#ataaaaat   2220

atagatactt tatatatgaa gaacacatca acaacagaca taatattaag ga

#acttaaga   2280

cctcacaccc tctataacat ttctgtaagg tcttacacca gatttggtca tg

#gcaatcag   2340

gtatcttctt tactctctgt aaggacttcg gagactgtgc ctgatagtgc ac

#cagaaaat   2400

atcacttaca aaaatatttc ttctggagag attgagctat cattccttcc cc

#caagtagt   2460

cccaatggaa tcataaaaaa atatacaatt tatctcaaga gaagtaatgg aa

#atgaggaa   2520

agaactataa atacaacctc tttaacccaa aacattaaag tactgaagaa at

#atacccaa   2580

tatatcattg aggtgtctgc tagtacactg aaaggtgaag gagttcggag tg

#ctcccata   2640

agtatactga cggaggaaga tgctcctgat tctccccctc aagacttctc tg

#taaaacag   2700

ttgtctggtg tcacggtgaa gttgtcatgg caaccacccc tggagccaaa tg

#gaattatc   2760

ctttattaca cagtttatgt ctggaataga tcatcattaa aaactattaa tg

#tcactgaa   2820

acatcattgg agttatcaga tttggattat aatgttgaat acagtgctta tg

#taacagct   2880

agcaccagat ttggtgatgg gaaaacagga agcaatatca ttagctttca aa

#caccagag   2940

ggagcaccaa gcgatcctcc caaagatgtt tattatgcaa acctcagttc tt

#catcaata   3000

attcttttct ggacacctcc ttcaaaacct aatgggatta tacaatatta ct

#ctgtttat   3060

tacagaaata cttcaggtac ttttatgcag aattttacac tccatgaact aa

#ccaatgac   3120

tttgacaata tgactgtatc cacaattata gataaactga caatattcag ct

#actataca   3180

ttttggttaa cagcaagtac ttcagttgga aatgggaata aaagcagtga ca

#tcattgaa   3240

gtatacacag atcaagacat acctgaaggg tttgttggaa acctgactta cg

#aatccatt   3300

tcgtcaactg caataaatgt aagctgggtc ccaccggctc aaccaaacgg tc

#tagtcttc   3360

tactatgttt cactgatctt acagcagact cctcgccatg tgagaccacc tc

#ttgttaca   3420

tatgagagaa gcatatattt tgataatctg gaaaaataca ctgattatat at

#taaaaatt   3480

actccatcaa cagaaaaggg attctctgat acctatactg cccagctata ca

#tcaagact   3540

gaagaagatg tcccagaaac ttcaccaata atcaacactt ttaaaaacct tt

#cctctacc   3600

tcagttctct tatcatggga tcccccagta aagccaaatg gtgcaataat aa

#gttatgat   3660

ttaactttac aaggaccaaa tgaaaattat tctttcatta cttctgataa tt

#acataata   3720

ttggaagagc tttcaccatt tacattatat agcttttttg ctgccgcaag aa

#ctagaaaa   3780

ggacttggtc cttccagtat tcttttcttt tacacagatg agtcagtgcc gt

#tagcacct   3840

ccacaaaatt tgactttaat caactgtact tcagactttg tatggctgaa at

#ggagccca   3900

agtcctcttc caggtggtat tgttaaagta tatagtttta aaattcatga ac

#atgaaact   3960

gacactatat attataagaa tatatcagga tttaaaactg aagccaaact tg

#ttggactg   4020

gaaccagtca gcacctactc tatccgtgta tctgcgttca ccaaagttgg aa

#atggcaat   4080

caatttagta atgtagtaaa attcacaacc caagaatcag ttccagatgt cg

#tgcagaat   4140

atgcagtgca tggcaactag ctggcagtca gttttagtga aatgggatcc ac

#ccaaaaag   4200

gcaaatggaa taataacgca gtatatggta acagttgaaa ggaattctac aa

#aagtttct   4260

ccccaagatc acatgtacac tttcataaag cttcttgcca atacctcata tg

#tctttaaa   4320

gtaagagctt caacctcagc tggtgaaggt gatgaaagca catgccatgt ca

#gcacacta   4380

cctgaaacag ttcccagtgt tcccacaaat attgcttttt ctgatgttca gt

#caactagt   4440

gcaacattga catggataag acctgacact atccttggct actttcaaaa tt

#acaaaatt   4500

accactcaac ttcgtgctca aaaatgcaaa gaatgggaat ccgaagaatg tg

#ttgaatat   4560

caaaaaattc aatacctcta tgaagctcac ttaactgaag agacagtata tg

#gattaaag   4620

aaatttagat ggtatagatt ccaagtggct gccagcacca atgctggcta tg

#gcaatgct   4680

tcaaactgga tttctacaaa aactctgcct ggccctccag atggtcctcc tg

#aaaatgtt   4740

catgtagtag caacatcacc ttttagcatc agcataagct ggagtgaacc tg

#ctgtcatt   4800

actggaccaa catgttatct gattgatgtc aaatcggtag ataatgatga at

#ttaatata   4860

tccttcatca agtcaaatga agaaaataaa accatagaaa ttaaagattt ag

#aaatattc   4920

acaaggtatt ctgtagtgat cactgcattt actgggaaca ttagtgctgc at

#atgtagaa   4980

gggaagtcaa gtgctgaaat gattgttact actttagaat cagccccaaa gg

#acccacct   5040

aacaacatga catttcagaa gataccagat gaagttacaa aatttcaatt aa

#cgttcctt   5100

cctccttctc aacctaatgg aaatatccaa gtatatcaag ctctggttta cc

#gagaagat   5160

gatcctactg ctgtccagat tcacaacctc agtattatac agaaaaccaa ca

#cattcgtc   5220

attgcaatgc tagaaggact aaaaggtgga catacataca atatcagtgt tt

#acgcagtc   5280

aatagtgctg gtgcaggtcc aaaggttccg atgagaataa ccatggatat ca

#aagctcca   5340

gcacgaccaa aaaccaaacc aacccctatt tatgatgcca caggaaaact gc

#ttgtgact   5400

tcaacaacaa ttacaatcag aatgccaata tgttactaca gtgatgatca tg

#gaccaata   5460

aaaaatgtac aagtgcttgc gacagaaaca ggagctcagc atgatggaaa tg

#taacaaag   5520

tggtatgatg catattttaa taaagcaagg ccatatttta caaatgaagg ct

#ttcctaac   5580

cctccatgta cagaaggaaa gacaaagttt agtggcaatg aagaaatcta ca

#tcataggt   5640

gctgataatg catgcatgat tcctggcaat gaagacaaaa tttgcaatgg ac

#cactgaaa   5700

ccaaaaaagc aatacttatt taaatttaga gctacaaata ttatgggaca at

#ttactgac   5760

tctgattatt ctgaccctgt taagacttta ggggaaggac tttcagaaag aa

#ccgtagag   5820

atcattcttt ccgtcacttt gtgtatcctt tcaataattc tccttggaac ag

#ctattttt   5880

gcatttgcaa gaattcgaca gaagcagaaa gaaggtggca catactctcc tc

#aggatgca   5940

gaaattattg acactaaatt gaagctggat cagctcatca cagtggcaga cc

#tggaactg   6000

aaggacgaga gattaacgcg gccaataagc aagaaatcct tcctgcaaca tg

#ttgaagag   6060

ctttgcacaa acaacaacct aaagtttcaa gaagaatttt cggaattacc aa

#aatttctt   6120

caggatcttt cttcaactga tgctgatctg ccttggaata gagcaaaaaa cc

#gtttccca   6180

aacataaaac catataataa taataacaga gtaaagctga tagctgacgc ta

#gtgttcca   6240

ggttcggatt atattaatgc cagctatatt tctggttatt tatgtccaaa tg

#aatttatt   6300

gctactcaag gtccactacc aggaacagtt ggagattttt ggagaatggt gt

#gggaaacc   6360

agggcaaaaa cattagtaat gctaacacag tgttttgaaa aaggacggat ca

#gatgccat   6420

cagtattggc cagaggacaa caagccagtt actgtctttg gagatatagt ga

#ttacaaag   6480

ctaatggagg atgttcaaat agattggact atcagggatc tgaaaattga aa

#ggcatggg   6540

gattgcatga ctgttcgaca gtgtaacttt actgcctggc cagagcatgg gg

#ttcctgag   6600

aacagcgccc ctctaattca ctttgtgaag ttggttcgag caagcagggc ac

#atgacacc   6660

acacctatga ttgttcactg cagtgctgga gttggaagaa ctggagtttt ta

#ttgctctg   6720

gaccatttaa cacaacatat aaatgaccat gattttgtgg atatatatgg ac

#tagtagct   6780

gaactgagaa gtgaaagaat gtgcatggtg cagaatctgg cacagtatat ct

#ttttacac   6840

cagtgcattc tggatctctt atcaaataag ggaagtaatc agcccatctg tt

#ttgttaac   6900

tattcagcac ttcagaagat ggactctttg gacgccatgg aaggtgatgt tg

#agcttgaa   6960

tgggaagaaa ccactatgta aatattcaga ccaaaggata caattggaag ag

#atttttaa   7020

atcccagggg ccaaagttac cccctcattc ttccgaattg aaatgtgcaa cc

#ttaaagaa   7080

atatctatgc ttctctcact gtgccttt

#

#           7108

<210> SEQ ID NO 2

<211> LENGTH: 2291

<212> TYPE: PRT

<213> ORGANISM: Human

<400> SEQUENCE: 2

Met Asp Phe Leu Ile Ile Phe Leu Leu Leu Ph

#e Ile Gly Thr Ser Glu

1               5

#                10

#                15

Thr Gln Val Asp Val Ser Asn Val Val Pro Gl

#y Thr Arg Tyr Asp Ile

20

#            25

#            30

Thr Ile Ser Ser Ile Ser Thr Thr Tyr Thr Se

#r Pro Val Thr Arg Ile

35

#        40

#        45

Val Thr Pro Asn Val Thr Lys Pro Gly Pro Pr

#o Val Phe Leu Ala Gly

50

#    55

#    60

Glu Arg Val Gly Ser Ala Gly Ile Leu Leu Se

#r Trp Asn Thr Pro Pro

65

#70

#75

#80

Asn Pro Asn Gly Arg Ile Ile Ser Tyr Ile Va

#l Lys Tyr Lys Glu Val

85

#                90

#                95

Cys Pro Trp Met Gln Thr Val Tyr Thr Gln Va

#l Arg Ser Lys Pro Asp

100

#           105

#           110

Ser Leu Glu Val Leu Leu Thr Asn Leu Asn Pr

#o Gly Thr Thr Tyr Glu

115

#       120

#       125

Ile Lys Val Ala Ala Glu Asn Ser Ala Gly Il

#e Gly Val Phe Ser Asp

130

#   135

#   140

Pro Phe Leu Phe Gln Thr Ala Glu Ser Ala Pr

#o Gly Lys Val Val Asn

145                 1

#50                 1

#55                 1

#60

Leu Thr Val Glu Ala Tyr Asn Ala Ser Ala Va

#l Lys Leu Ile Trp Tyr

165

#               170

#               175

Leu Pro Arg Gln Pro Asn Gly Lys Ile Thr Se

#r Phe Lys Ile Ser Val

180

#           185

#           190

Lys His Ala Arg Ser Gly Ile Val Val Lys As

#p Val Ser Ile Arg Val

195

#       200

#       205

Glu Asp Ile Leu Thr Gly Lys Leu Pro Glu Cy

#s Asn Glu Asn Ser Glu

210

#   215

#   220

Ser Phe Leu Trp Ser Thr Ala Ser Pro Ser Pr

#o Thr Leu Gly Arg Val

225                 2

#30                 2

#35                 2

#40

Thr Pro Pro Ser Arg Thr Thr His Ser Ser Se

#r Thr Leu Thr Gln Asn

245

#               250

#               255

Glu Ile Ser Ser Val Trp Lys Glu Pro Ile Se

#r Phe Val Val Thr His

260

#           265

#           270

Leu Arg Pro Tyr Thr Thr Tyr Leu Phe Glu Va

#l Ser Ala Ala Thr Thr

275

#       280

#       285

Glu Ala Gly Tyr Ile Asp Ser Thr Ile Val Ar

#g Thr Pro Glu Ser Val

290

#   295

#   300

Pro Glu Gly Pro Pro Gln Asn Cys Val Thr Gl

#y Asn Ile Thr Gly Lys

305                 3

#10                 3

#15                 3

#20

Ser Phe Ser Ile Leu Trp Asp Pro Pro Thr Il

#e Val Thr Gly Lys Phe

325

#               330

#               335

Ser Tyr Arg Val Glu Leu Tyr Gly Pro Ser Gl

#y Arg Ile Leu Asp Asn

340

#           345

#           350

Ser Thr Lys Asp Leu Lys Phe Ala Phe Thr As

#n Leu Thr Pro Phe Thr

355

#       360

#       365

Met Tyr Asp Val Tyr Ile Ala Ala Glu Thr Se

#r Ala Gly Thr Gly Pro

370

#   375

#   380

Lys Ser Asn Ile Ser Val Phe Thr Pro Pro As

#p Val Pro Gly Ala Val

385                 3

#90                 3

#95                 4

#00

Phe Asp Leu Gln Leu Ala Glu Val Glu Ser Th

#r Gln Val Arg Ile Thr

405

#               410

#               415

Trp Lys Lys Pro Arg Gln Pro Asn Gly Ile Il

#e Asn Gln Tyr Arg Val

420

#           425

#           430

Lys Val Leu Val Pro Glu Thr Gly Ile Ile Le

#u Glu Asn Thr Leu Leu

435

#       440

#       445

Thr Gly Asn Asn Glu Tyr Ile Asn Asp Pro Me

#t Ala Pro Glu Ile Val

450

#   455

#   460

Asn Ile Val Glu Pro Met Val Gly Leu Tyr Gl

#u Gly Ser Ala Glu Met

465                 4

#70                 4

#75                 4

#80

Ser Ser Asp Leu His Ser Leu Ala Thr Phe Il

#e Tyr Asn Ser His Pro

485

#               490

#               495

Asp Lys Asn Phe Pro Ala Arg Asn Arg Ala Gl

#u Asp Gln Thr Ser Pro

500

#           505

#           510

Val Val Thr Thr Arg Asn Gln Tyr Ile Thr As

#p Ile Ala Ala Glu Gln

515

#       520

#       525

Leu Ser Tyr Val Ile Arg Arg Leu Val Pro Ph

#e Thr Glu His Met Ile

530

#   535

#   540

Ser Val Ser Ala Phe Thr Ile Met Gly Glu Gl

#y Pro Pro Thr Val Leu

545                 5

#50                 5

#55                 5

#60

Ser Val Arg Thr Arg Gln Gln Val Pro Ser Se

#r Ile Lys Ile Ile Asn

565

#               570

#               575

Tyr Lys Asn Ile Ser Ser Ser Ser Ile Leu Le

#u Tyr Trp Asp Pro Pro

580

#           585

#           590

Glu Tyr Pro Asn Gly Lys Ile Thr His Tyr Th

#r Ile Tyr Ala Met Glu

595

#       600

#       605

Leu Asp Thr Asn Arg Ala Phe Gln Ile Thr Th

#r Ile Asp Asn Ser Phe

610

#   615

#   620

Leu Ile Thr Gly Leu Lys Lys Tyr Thr Lys Ty

#r Lys Met Arg Val Ala

625                 6

#30                 6

#35                 6

#40

Ala Ser Thr His Asp Gly Glu Ser Ser Leu Se

#r Glu Glu Asn Asp Ile

645

#               650

#               655

Phe Val Arg Thr Ser Glu Asp Glu Pro Glu Se

#r Ser Pro Gln Asp Val

660

#           665

#           670

Glu Val Ile Asp Val Thr Ala Asp Glu Ile Ar

#g Leu Lys Trp Ser Pro

675

#       680

#       685

Pro Glu Lys Pro Asn Gly Ile Ile Ile Ala Ty

#r Glu Val Leu Tyr Lys

690

#   695

#   700

Asn Ile Asp Thr Leu Tyr Met Lys Asn Thr Se

#r Thr Thr Asp Ile Ile

705                 7

#10                 7

#15                 7

#20

Leu Arg Asn Leu Arg Pro His Thr Leu Tyr As

#n Ile Ser Val Arg Ser

725

#               730

#               735

Tyr Thr Arg Phe Gly His Gly Asn Gln Val Se

#r Ser Leu Leu Ser Val

740

#           745

#           750

Arg Thr Ser Glu Thr Val Pro Asp Ser Ala Pr

#o Glu Asn Ile Thr Tyr

755

#       760

#       765

Lys Asn Ile Ser Ser Gly Glu Ile Glu Leu Se

#r Phe Leu Pro Pro Ser

770

#   775

#   780

Ser Pro Asn Gly Ile Ile Lys Lys Tyr Thr Il

#e Tyr Leu Lys Arg Ser

785                 7

#90                 7

#95                 8

#00

Asn Gly Asn Glu Glu Arg Thr Ile Asn Thr Th

#r Ser Leu Thr Gln Asn

805

#               810

#               815

Ile Lys Val Leu Lys Lys Tyr Thr Gln Tyr Il

#e Ile Glu Val Ser Ala

820

#           825

#           830

Ser Thr Leu Lys Gly Glu Gly Val Arg Ser Al

#a Pro Ile Ser Ile Leu

835

#       840

#       845

Thr Glu Glu Asp Ala Pro Asp Ser Pro Pro Gl

#n Asp Phe Ser Val Lys

850

#   855

#   860

Gln Leu Ser Gly Val Thr Val Lys Leu Ser Tr

#p Gln Pro Pro Leu Glu

865                 8

#70                 8

#75                 8

#80

Pro Asn Gly Ile Ile Leu Tyr Tyr Thr Val Ty

#r Val Trp Asn Arg Ser

885

#               890

#               895

Ser Leu Lys Thr Ile Asn Val Thr Glu Thr Se

#r Leu Glu Leu Ser Asp

900

#           905

#           910

Leu Asp Tyr Asn Val Glu Tyr Ser Ala Tyr Va

#l Thr Ala Ser Thr Arg

915

#       920

#       925

Phe Gly Asp Gly Lys Thr Gly Ser Asn Ile Il

#e Ser Phe Gln Thr Pro

930

#   935

#   940

Glu Gly Ala Pro Ser Asp Pro Pro Lys Asp Va

#l Tyr Tyr Ala Asn Leu

945                 9

#50                 9

#55                 9

#60

Ser Ser Ser Ser Ile Ile Leu Phe Trp Thr Pr

#o Pro Ser Lys Pro Asn

965

#               970

#               975

Gly Ile Ile Gln Tyr Tyr Ser Val Tyr Tyr Ar

#g Asn Thr Ser Gly Thr

980

#           985

#           990

Phe Met Gln Asn Phe Thr Leu His Glu Leu Th

#r Asn Asp Phe Asp Asn

995

#       1000

#      1005

Met Thr Val Ser Thr Ile Ile Asp Lys Leu Th

#r Ile Phe Ser Tyr Tyr

1010

#   1015

#  1020

Thr Phe Trp Leu Thr Ala Ser Thr Ser Val Gl

#y Asn Gly Asn Lys Ser

1025                1030

#                1035

#               1040

Ser Asp Ile Ile Glu Val Tyr Thr Asp Gln As

#p Ile Pro Glu Gly Phe

1045

#               1050

#              1055

Val Gly Asn Leu Thr Tyr Glu Ser Ile Ser Se

#r Thr Ala Ile Asn Val

1060

#           1065

#          1070

Ser Trp Val Pro Pro Ala Gln Pro Asn Gly Le

#u Val Phe Tyr Tyr Val

1075

#       1080

#      1085

Ser Leu Ile Leu Gln Gln Thr Pro Arg His Va

#l Arg Pro Pro Leu Val

1090

#   1095

#  1100

Thr Tyr Glu Arg Ser Ile Tyr Phe Asp Asn Le

#u Glu Lys Tyr Thr Asp

1105                1110

#                1115

#               1120

Tyr Ile Leu Lys Ile Thr Pro Ser Thr Glu Ly

#s Gly Phe Ser Asp Thr

1125

#               1130

#              1135

Tyr Thr Ala Gln Leu Tyr Ile Lys Thr Glu Gl

#u Asp Val Pro Glu Thr

1140

#           1145

#          1150

Ser Pro Ile Ile Asn Thr Phe Lys Asn Leu Se

#r Ser Thr Ser Val Leu

1155

#       1160

#      1165

Leu Ser Trp Asp Pro Pro Val Lys Pro Asn Gl

#y Ala Ile Ile Ser Tyr

1170

#   1175

#  1180

Asp Leu Thr Leu Gln Gly Pro Asn Glu Asn Ty

#r Ser Phe Ile Thr Ser

1185                1190

#                1195

#               1200

Asp Asn Tyr Ile Ile Leu Glu Glu Leu Ser Pr

#o Phe Thr Leu Tyr Ser

1205

#               1210

#              1215

Phe Phe Ala Ala Ala Arg Thr Arg Lys Gly Le

#u Gly Pro Ser Ser Ile

1220

#           1225

#          1230

Leu Phe Phe Tyr Thr Asp Glu Ser Val Pro Le

#u Ala Pro Pro Gln Asn

1235

#       1240

#      1245

Leu Thr Leu Ile Asn Cys Thr Ser Asp Phe Va

#l Trp Leu Lys Trp Ser

1250

#   1255

#  1260

Pro Ser Pro Leu Pro Gly Gly Ile Val Lys Va

#l Tyr Ser Phe Lys Ile

1265                1270

#                1275

#               1280

His Glu His Glu Thr Asp Thr Ile Tyr Tyr Ly

#s Asn Ile Ser Gly Phe

1285

#               1290

#              1295

Lys Thr Glu Ala Lys Leu Val Gly Leu Glu Pr

#o Val Ser Thr Tyr Ser

1300

#           1305

#          1310

Ile Arg Val Ser Ala Phe Thr Lys Val Gly As

#n Gly Asn Gln Phe Ser

1315

#       1320

#      1325

Asn Val Val Lys Phe Thr Thr Gln Glu Ser Va

#l Pro Asp Val Val Gln

1330

#   1335

#  1340

Asn Met Gln Cys Met Ala Thr Ser Trp Gln Se

#r Val Leu Val Lys Trp

1345                1350

#                1355

#               1360

Asp Pro Pro Lys Lys Ala Asn Gly Ile Ile Th

#r Gln Tyr Met Val Thr

1365

#               1370

#              1375

Val Glu Arg Asn Ser Thr Lys Val Ser Pro Gl

#n Asp His Met Tyr Thr

1380

#           1385

#          1390

Phe Ile Lys Leu Leu Ala Asn Thr Ser Tyr Va

#l Phe Lys Val Arg Ala

1395

#       1400

#      1405

Ser Thr Ser Ala Gly Glu Gly Asp Glu Ser Th

#r Cys His Val Ser Thr

1410

#   1415

#  1420

Leu Pro Glu Thr Val Pro Ser Val Pro Thr As

#n Ile Ala Phe Ser Asp

1425                1430

#                1435

#               1440

Val Gln Ser Thr Ser Ala Thr Leu Thr Trp Il

#e Arg Pro Asp Thr Ile

1445

#               1450

#              1455

Leu Gly Tyr Phe Gln Asn Tyr Lys Ile Thr Th

#r Gln Leu Arg Ala Gln

1460

#           1465

#          1470

Lys Cys Lys Glu Trp Glu Ser Glu Glu Cys Va

#l Glu Tyr Gln Lys Ile

1475

#       1480

#      1485

Gln Tyr Leu Tyr Glu Ala His Leu Thr Glu Gl

#u Thr Val Tyr Gly Leu

1490

#   1495

#  1500

Lys Lys Phe Arg Trp Tyr Arg Phe Gln Val Al

#a Ala Ser Thr Asn Ala

1505                1510

#                1515

#               1520

Gly Tyr Gly Asn Ala Ser Asn Trp Ile Ser Th

#r Lys Thr Leu Pro Gly

1525

#               1530

#              1535

Pro Pro Asp Gly Pro Pro Glu Asn Val His Va

#l Val Ala Thr Ser Pro

1540

#           1545

#          1550

Phe Ser Ile Ser Ile Ser Trp Ser Glu Pro Al

#a Val Ile Thr Gly Pro

1555

#       1560

#      1565

Thr Cys Tyr Leu Ile Asp Val Lys Ser Val As

#p Asn Asp Glu Phe Asn

1570

#   1575

#  1580

Ile Ser Phe Ile Lys Ser Asn Glu Glu Asn Ly

#s Thr Ile Glu Ile Lys

1585                1590

#                1595

#               1600

Asp Leu Glu Ile Phe Thr Arg Tyr Ser Val Va

#l Ile Thr Ala Phe Thr

1605

#               1610

#              1615

Gly Asn Ile Ser Ala Ala Tyr Val Glu Gly Ly

#s Ser Ser Ala Glu Met

1620

#           1625

#          1630

Ile Val Thr Thr Leu Glu Ser Ala Pro Lys As

#p Pro Pro Asn Asn Met

1635

#       1640

#      1645

Thr Phe Gln Lys Ile Pro Asp Glu Val Thr Ly

#s Phe Gln Leu Thr Phe

1650

#   1655

#  1660

Leu Pro Pro Ser Gln Pro Asn Gly Asn Ile Gl

#n Val Tyr Gln Ala Leu

1665                1670

#                1675

#               1680

Val Tyr Arg Glu Asp Asp Pro Thr Ala Val Gl

#n Ile His Asn Leu Ser

1685

#               1690

#              1695

Ile Ile Gln Lys Thr Asn Thr Phe Val Ile Al

#a Met Leu Glu Gly Leu

1700

#           1705

#          1710

Lys Gly Gly His Thr Tyr Asn Ile Ser Val Ty

#r Ala Val Asn Ser Ala

1715

#       1720

#      1725

Gly Ala Gly Pro Lys Val Pro Met Arg Ile Th

#r Met Asp Ile Lys Ala

1730

#   1735

#  1740

Pro Ala Arg Pro Lys Thr Lys Pro Thr Pro Il

#e Tyr Asp Ala Thr Gly

1745                1750

#                1755

#               1760

Lys Leu Leu Val Thr Ser Thr Thr Ile Thr Il

#e Arg Met Pro Ile Cys

1765

#               1770

#              1775

Tyr Tyr Ser Asp Asp His Gly Pro Ile Lys As

#n Val Gln Val Leu Ala

1780

#           1785

#          1790

Thr Glu Thr Gly Ala Gln His Asp Gly Asn Va

#l Thr Lys Trp Tyr Asp

1795

#       1800

#      1805

Ala Tyr Phe Asn Lys Ala Arg Pro Tyr Phe Th

#r Asn Glu Gly Phe Pro

1810

#   1815

#  1820

Asn Pro Pro Cys Thr Glu Gly Lys Thr Lys Ph

#e Ser Gly Asn Glu Glu

1825                1830

#                1835

#               1840

Ile Tyr Ile Ile Gly Ala Asp Asn Ala Cys Me

#t Ile Pro Gly Asn Glu

1845

#               1850

#              1855

Asp Lys Ile Cys Asn Gly Pro Leu Lys Pro Ly

#s Lys Gln Tyr Leu Phe

1860

#           1865

#          1870

Lys Phe Arg Ala Thr Asn Ile Met Gly Gln Ph

#e Thr Asp Ser Asp Tyr

1875

#       1880

#      1885

Ser Asp Pro Val Lys Thr Leu Gly Glu Gly Le

#u Ser Glu Arg Thr Val

1890

#   1895

#  1900

Glu Ile Ile Leu Ser Val Thr Leu Cys Ile Le

#u Ser Ile Ile Leu Leu

1905                1910

#                1915

#               1920

Gly Thr Ala Ile Phe Ala Phe Ala Arg Ile Ar

#g Gln Lys Gln Lys Glu

1925

#               1930

#              1935

Gly Gly Thr Tyr Ser Pro Gln Asp Ala Glu Il

#e Ile Asp Thr Lys Leu

1940

#           1945

#          1950

Lys Leu Asp Gln Leu Ile Thr Val Ala Asp Le

#u Glu Leu Lys Asp Glu

1955

#       1960

#      1965

Arg Leu Thr Arg Pro Ile Ser Lys Lys Ser Ph

#e Leu Gln His Val Glu

1970

#   1975

#  1980

Glu Leu Cys Thr Asn Asn Asn Leu Lys Phe Gl

#n Glu Glu Phe Ser Glu

1985                1990

#                1995

#               2000

Leu Pro Lys Phe Leu Gln Asp Leu Ser Ser Th

#r Asp Ala Asp Leu Pro

2005

#               2010

#              2015

Trp Asn Arg Ala Lys Asn Arg Phe Pro Asn Il

#e Lys Pro Tyr Asn Asn

2020

#           2025

#          2030

Asn Asn Arg Val Lys Leu Ile Ala Asp Ala Se

#r Val Pro Gly Ser Asp

2035

#       2040

#      2045

Tyr Ile Asn Ala Ser Tyr Ile Ser Gly Tyr Le

#u Cys Pro Asn Glu Phe

2050

#   2055

#  2060

Ile Ala Thr Gln Gly Pro Leu Pro Gly Thr Va

#l Gly Asp Phe Trp Arg

2065                2070

#                2075

#               2080

Met Val Trp Glu Thr Arg Ala Lys Thr Leu Va

#l Met Leu Thr Gln Cys

2085

#               2090

#              2095

Phe Glu Lys Gly Arg Ile Arg Cys His Gln Ty

#r Trp Pro Glu Asp Asn

2100

#           2105

#          2110

Lys Pro Val Thr Val Phe Gly Asp Ile Val Il

#e Thr Lys Leu Met Glu

2115

#       2120

#      2125

Asp Val Gln Ile Asp Trp Thr Ile Arg Asp Le

#u Lys Ile Glu Arg His

2130

#   2135

#  2140

Gly Asp Cys Met Thr Val Arg Gln Cys Asn Ph

#e Thr Ala Trp Pro Glu

2145                2150

#                2155

#               2160

His Gly Val Pro Glu Asn Ser Ala Pro Leu Il

#e His Phe Val Lys Leu

2165

#               2170

#              2175

Val Arg Ala Ser Arg Ala His Asp Thr Thr Pr

#o Met Ile Val His Cys

2180

#           2185

#          2190

Ser Ala Gly Val Gly Arg Thr Gly Val Phe Il

#e Ala Leu Asp His Leu

2195

#       2200

#      2205

Thr Gln His Ile Asn Asp His Asp Phe Val As

#p Ile Tyr Gly Leu Val

2210

#   2215

#  2220

Ala Glu Leu Arg Ser Glu Arg Met Cys Met Va

#l Gln Asn Leu Ala Gln

2225                2230

#                2235

#               2240

Tyr Ile Phe Leu His Gln Cys Ile Leu Asp Le

#u Leu Ser Asn Lys Gly

2245

#               2250

#              2255

Ser Asn Gln Pro Ile Cys Phe Val Asn Tyr Se

#r Ala Leu Gln Lys Met

2260

#           2265

#          2270

Asp Ser Leu Asp Ala Met Glu Gly Asp Val Gl

#u Leu Glu Trp Glu Glu

2275

#       2280

#      2285

Thr Thr Met

2290

<210> SEQ ID NO 3

<211> LENGTH: 254366

<212> TYPE: DNA

<213> ORGANISM: Human

<220> FEATURE:

<221> NAME/KEY: misc_feature

<222> LOCATION: (1)...(254366)

<223> OTHER INFORMATION: n = A,T,C or G

<400> SEQUENCE: 3

cattatctat ggaacataat ctgaggcttt ttttttacag ttggtagata ct

#tatgtaca     60

agattttgct gtgaaaatca gggcaagaag gtagtgatgc aaggtagcag at

#aacattga    120

aatacatttt tgaaaataat ttttaaaatt gatgtaatgc aattagatta ct

#tgagctaa    180

tagcatagct ttattttatt ttatttattt tattttattt atttttttga ga

#cacagtct    240

tgctctgttg cccaggctgg agtgcagttg ccgatattgg ctcacttcag cc

#tccgcctc    300

ctgagctcaa gcaattctcg tgcctcagcc tcctgaaagc tgggaccaca gg

#tctgccca    360

ccacgcaggg ctaatttttt tatttttagt ggagacaggg ttttgccatg tt

#gcccaggc    420

tgaccttaaa ctcctggtct caagtgatcc aaccgccttg gtctcccgaa gt

#gccggcat    480

tacaggtgtg agccaccaca ctcgtcttaa ttgcatagtt ttagagatcc at

#gttggatt    540

agctcttctg tagtgtcctg atgacctgtg accaatgata agagtatgga at

#taggtagt    600

ttctatgaaa aggcatcttt tctggcgact aatagccaca gtatcaagag tt

#ttaaaagc    660

ccctctcctc tggtctcaat ggagtcaaag aaaactctcc cgactgctcc tg

#aaaaagga    720

tgtcaaatga aactgtttca aattgctgaa tagcctggct aatcctgcct gt

#ctccaatc    780

acatactccg aatccatatt tttctcactg tggtaagctt tccaactatt tt

#tcagaaaa    840

caaaacttat atttggaata acttggtgct tctgtggcag taaaaccatg ac

#tgaaatgt    900

atctctgtgg aaacccttac ttcatttaaa atttattatt cctcctaatg at

#tcaaggct    960

tcaaatattt caatggtaaa gaaagagctt tttctattca gagggaatat tc

#atttactt   1020

cttcagtgct ttgtgtgtct aaagtagaaa aatatgagat tacatgagac at

#atactttt   1080

tcagtgttac tgatcatatt cccttatcta aattctttaa taactaactt ta

#ttattcct   1140

aaaatataaa taaaaaataa tgcacatttt tcagcatcac tatacacatg tt

#catttttt   1200

ggttttagat attaatctat acccagttca aactgtggaa actgaactaa ca

#tgactgaa   1260

ataaaatagt gttatatttt gttctttaga ctcttttttc ccttcctgag at

#tttgatat   1320

gtatttggag agttttgagt caatatttat ttgatttgtt ttcttttctg ga

#gtgatatt   1380

gtaaatactt taaagatttt gattgagtga gaggtgtgag ctatattttc tt

#ctttcctg   1440

tatgatatac atacattgtt tccaatctaa tttctattaa ataactatag ga

#gagcccac   1500

agccttgtta ttttacatat cactatttag atatttgtta tttatttatt tg

#tgttggcc   1560

tgaagtaaat gttacttttg tacgatattt gaaggataga tttattttat aa

#attaatag   1620

tttaaataag attttgccag catttgaaat gaacaaatgt ttggacaatg aa

#aacatcag   1680

tatgaaaggg aatactgtaa ttactttagt acatagtatt ccttaatatc ca

#ttaaaatt   1740

ggtccaagca aactctaatt atgaacatca tattaacatt tgatctaatt ac

#tgaatata   1800

attaaaagca aaataagtta atttactaaa gaattctgaa atttactatt tt

#cagtattt   1860

caggataacc aacatctttt ttctattaat ctagaataaa tttccatata tt

#aatgttgt   1920

ttacttttaa tgttagtgtg ctcaaaaagt attgttaact tttaaaattc aa

#ttctacag   1980

ataatattct ttttatctca ggaatagatc atcattaaaa actattaatg tc

#actgaaac   2040

atcattggag ttatcagatt tggattataa tgttgaatac agtgcttatg ta

#acagctag   2100

caccagattt ggtgatggga aaacaagaag caatatcatt agctttcaaa ca

#ccagaggg   2160

aggtgagtta aggatgtatg ccaattaaaa gaatgttctt tttctttaaa aa

#aaaaatcc   2220

tgcccagaaa aatattcaaa tatcaaaatg tatgatgaag cctaatattc at

#catcagtt   2280

tgatgaaaaa ttgcattttg atcacttttt agctgtgtga tgttgggaaa at

#taatctct   2340

gtgtgcctca atttcccatt ggtaatgtgg aaacagtatc attctacttc ac

#agggttct   2400

tatgaagatt acataagttt atatttttaa aagcacttag tacagaagtt ac

#ttggacaa   2460

atagaatttt atgtgtttat taaacgaaac aacataaaat gcatgaatca tt

#tgtctatg   2520

acttttatta ttcaatataa aaattctaag ttatattaga atttcaaatt at

#gtattttg   2580

tattggaaac ctgttataat attgttctca tatccagagc agtggacagg tt

#ttagaacg   2640

gagatagtat tttatgggta agaaatctat ctgtcttcag cttgaatatg cc

#tataataa   2700

agtattagag gggtgaccca atgtgtttta tggatttcat ttctgacatt tc

#taattcaa   2760

gcttttttga aaaacatttt ttatcacttt aatttataaa ctgtaggtaa aa

#ttcaggcc   2820

atttcagaca ttacttgtaa acacaaatac agtaatttgt tcaattattt gt

#tttatagc   2880

accaagcgat cctcccaaag atgtttatta tgcaaacctc agttcttcat ca

#ataattct   2940

tttctggaca cctccttcaa aacctaatgg gattatacaa tattactctg tt

#tattacag   3000

aaatacttca ggtactttta tgcaggtaag aactgaattt tcttctagtt ct

#ttattaac   3060

atccttaagt tttattaata atacagactt gtcacagtaa aagaaattgt tt

#accttaca   3120

ttgataatta ggcacagatg tattttataa aactcccatt gacatagaaa aa

#tgcggtgt   3180

agaaatgtca gatacattta atctctcttt acagacacac acacacacac at

#acaacttc   3240

tatataagct tcacatgtat taaaaatagt gaatctgcca cctactgaaa at

#tctgttta   3300

taaagatggc cctcaattac acttcctcca ataagtgttc tctaaagtgc tg

#atggtatc   3360

atttatcctc aaagttattt attagctaaa ttttttttca tttgtttgta ta

#tgatataa   3420

atagttctag tgtttggatg tgtttgtttt tctttaatta aaaaaagttt tt

#gatagcag   3480

gaagggttat tataataata gtatattagt agttaatgtt taatgtcaga tg

#aaatgaag   3540

accactcgga atgtgtttaa ttaatttgtc atagataaga ttctaggctt gc

#acagtttg   3600

tagatgggca ctctctagga tgtgaatgat gatggctatg aaaatagcta ac

#atgcattt   3660

actttgaaaa aatattttca attttcaaca gaattatatt atttcttcaa at

#tagatgtt   3720

tcacagaact ctaacatata aaaaggataa ttggaatgat tatgattgaa tc

#aaagatgc   3780

agagagctgg aatataatta gaaaaacacg gccgggcgtg gtggctcacg cc

#tgtaatcc   3840

cagcactttg ggaggccgag gcgggcggat cacaaggtca ggagatcgag at

#catcctgg   3900

ctaacacggt gaaaccccgt ctctactaaa aatacaaaaa attagccagg cg

#tcgtggca   3960

ggcgcctgta gtcccagcta ctggggaggc tgaggcagga gaatggcgtc aa

#cccgggag   4020

gcggagcttg cagtgagctg agatcccgcc actgcactcc agcctgggcg ag

#agagcgag   4080

actccatctc aaaagaaaaa gaaaaacacg aatttagaag aaatgctgca at

#gtacagaa   4140

tacatccctt agtggtagaa attattgacc acatgtttgt gtcttaggtg at

#tcttaatt   4200

atttctatcc ttttaagtaa aagaagaaga aagataagtc ttacaaattc tg

#agttacct   4260

aatcccattt gtgactgaca gcccaagttt agtcactagt tagctctact ga

#gtaacagc   4320

ctctgtaatt aagactttag tgcagctata gtgcaatgta ggctaatgaa ga

#gggcaaga   4380

gcagaacttg caagctatct caggactaac ctagcaggga gaaagacaaa gt

#ccagaagg   4440

tggtttagtg tttatattct gttctataag agtagggttg tataagtctg tc

#tatttaaa   4500

acttgatgca aagagaaaac tactttataa aagacatgta gatataatta ta

#gctgtaat   4560

gaaagacatg tagatatagt tacagatgta atcgtaaatc aacatttttg aa

#caaatgcc   4620

ttaagagcag aaggagaaag gaaggtctag ttttctactc tctatgtcac gc

#agtttttg   4680

ctttttgttt tgttctctgt agggaagaga aatggggcct agagaggcaa tt

#tatttttt   4740

aaccaaaatg ttgtttacaa ttgtaacaat atgtcattat acccatagaa ga

#tatgcaaa   4800

ttggagattt tccttctttt atgcatttaa aaaacattgc acaattgttc ca

#gtagttct   4860

aaattttagc aatcattttg tctctgtaca atttacttat ggcttctatg tg

#atttatat   4920

tttggttctc tttatccata tctaaataat atagcataag tatcaaacta tg

#gttccaac   4980

gtgatcttct aaacctactt attcacacct gggtgtgtaa tatgatctaa tt

#tgcaattc   5040

atctgcctta gaacatgtta tcttttatta aataatctta agaatgcttt ta

#agtgtgac   5100

agctgcaaga gggcacaggc taatgatgtt aaaatatttc agaagtatag tc

#tcatattg   5160

cttgaagttt atccgtgctt taacttattc ctaaagttaa tgttaaaaat ag

#catcaata   5220

ccttcactac ctaattttct attttgaatt agtggaagaa agcctcaaaa tg

#aaaattat   5280

gtagcagaat aagtgtatac ctttttattt gttccttatc atctttcccc tt

#cctacaga   5340

actttgtaga atatgtcatg cgtggcatat catgttctgc ctcctattac cg

#ataactgt   5400

tgcttctctt agttccctta tgccatgaca agcatcttgt agaaaaagaa tt

#gtgtaata   5460

tttatttttt catctccaaa agtcttctgc aactatgtca gacataggtt ta

#atgctcaa   5520

tacatatttt aattgaaaga tttaaaaaat attatagtag accaacatca ct

#tttagtac   5580

atagtcataa ttttggagcc cttgagtatg tagcaaagcc atctttcctt tt

#tcttatct   5640

tggagaattt aacctctttg ctactacttg gcaatccata ttgttcttcc tt

#cagttgtt   5700

gcacattgta ttttgtacag catattaact tttctacttt ttaagtttta cc

#cacttatg   5760

tttccttagt gtgcctggca taatgtcttc tattttaaaa agtgttaaat gg

#gccgggtg   5820

tggtggctca cgcctgtaat cccagcactt tgggaggctg aagcaggtgg at

#cacgaggt   5880

caggagatcg agaccatcct ggctaacaag gtgaaaccct gtctctacta aa

#aatacaaa   5940

aaattagccg ggcatggtgg caggctcctg tagtcccagg tactcaggag gc

#tgaggcag   6000

gagaatggtg tgaacctggg aggtggaggt tgcagtgagc cgagatcgtg cc

#actgccct   6060

ctagcctggg caacagagtg agactctgtt ttaaagaaaa aaaaagtgtt aa

#atgaatat   6120

tagttggttg gtcaaatttg aaaaagtttt actaaatacc ttctgactat at

#ttatataa   6180

acaaaagaat aagccttact tagataattt gtgccaaaag acattttgtt tt

#tgcaaaaa   6240

taaacagctg aataaaataa tcatctggat aattgattta atgttacaaa tt

#tgttacat   6300

gcctatgcac attaagtcac acagtcagca ggaatgactt ctgggtgatt ca

#gataattt   6360

gttatgtatt agccatcaag gtcataggta atcagaataa attctataac aa

#aaattaaa   6420

atttacatca aaaagctatg ttaatacttt taagtggtgc tttatataag cc

#agttgttc   6480

catgtgtaaa gtagatgtat tggaaggtat taaagttcat ggatcatatt tt

#gtgtgaga   6540

ttgctatatt actttaactt gtctatttct atgtaaatca ccacaaaatt gt

#agtaaatc   6600

ttttattgca ctatattttt ccctgaatac tggcaaaaga accataaaat tt

#tgctaatt   6660

taatttgttg ataaatttca gaaccattct tactataaat ttggtaaatt tg

#cttatcct   6720

atgttattca tttaaatgaa actaattact gttttttttt aattgtgtgc ta

#gacatggt   6780

attaatggct ttttgtgctt catgtcatct aatcctcaca agttgtctgt ga

#agtggaga   6840

tgattatttc cattttacag ttgaaggaag aaaagctttg agattaaatg at

#ttatccag   6900

gataccctga cagaatttga atgcaggtct ataggactta aatggcttca tg

#tggattgg   6960

aatgattcag gttactctgc agatggaaat tataaaatta ttcatactga tt

#agctatgt   7020

gtttaagtct ccttttattt tagaattaat tttatttggc tatatgtttt at

#ttttaaaa   7080

tttgatagga aagaaaatga ttacatacat accctaatac ttttttttaa gc

#cttgggga   7140

aaaatgcaac tgggagtcag tcaagagaat ttaaaacttt ctcttactct ac

#acatcaga   7200

gagtacatca gtctgctatc cctttgctac aactgtgaga agtaaagtct ga

#aaagaaat   7260

gtgaaagtct gaaagcccac taaatgtgaa taataatagc gatttgagtt ca

#tagaaaca   7320

ggcaaacaca ttcgaaattc cttcatcaca aatggaaaga aacacaatta aa

#agttttct   7380

aatactccca aacttgttta aaattagctg atgctttgaa aattacttga at

#gtttttat   7440

aaggaaagtg atgctgatca gcacagttgt agcatttcca tttggccact tg

#acattctt   7500

cattgttggc ttggagtttt tattctttgc tatttttttg tattggcttt gc

#aataaaaa   7560

ccaccatcta tttctctttt agtacaaaca tatttccagt ttaattgttg ca

#ataaaaaa   7620

tgttctatgt cgattttcct aaaacaacat attaaaataa tgataaataa ta

#aaatcgat   7680

ccattgataa caattagttt gaagtgttca tgcatactaa aaaaatacat tc

#tgaacaat   7740

gaatgtgttt atttttcaga attttacact ccatgaagta accaatgact tt

#gacaatat   7800

gactgtatcc acaattatag ataaactgac aatattcagc tactatacat tt

#tggttaac   7860

agcaagtact tcagttggaa atgggaataa aagcagtgac atcattgaag ta

#tacacaga   7920

tcaagacagt atgtaaacaa aaaacactaa tctttaatat gattaattta aa

#acttatta   7980

ttttaggaaa ttttactatt tgtttgaatt tgtaataaca tcttttattt ag

#acacgttc   8040

attataggag tttgaaaatg caattaatat acttacaaaa ctattgcagt aa

#tagcctct   8100

tctgttcaag aaaactgcta acatccattc atgaaaattc tgttcttttt at

#tgcttcaa   8160

aagatgtcgt ggccatccag ttatgggcac aaaaagtact gcatacatgg at

#gaattttc   8220

cagtagttaa tttatttatt cattttcctt aaggacttaa aaaatctcta gc

#aacttgtt   8280

ttcttttcag actttgaatc tacacaggac tctgcagcac atctcttctc ac

#tgtgtttg   8340

tgactaatat atccagagta ttttccttaa ctccagaagt ttctcgtatg ca

#tcttctga   8400

agaatcctat ttatcccgag tattcagaaa actataatga ttgaagatct tg

#atgttttt   8460

tatgtttcaa ttttcagaat acagtgataa gtggatcatt gcctattttt ct

#tgtagttg   8520

tttctgtcat ccatttgctt attttcaaag attaatccct tattgagaag tg

#gcagtgac   8580

ctaaacttct ggagtaaaac tccatgttta ttatctgaaa gccataaatt ga

#cagattcc   8640

ttaagcatga gaagtgaatg cttgatttgt tgttggaaca gtcttttgaa tt

#gttgacaa   8700

gttggtcaac ataaaaataa atgataaatg tggggaaata tgtatttggg ga

#gtctttag   8760

caaaaatgtt atattgtaat atatgatcaa taccatttca ggtattcttt aa

#atgcagat   8820

ctctcccagg atttttgcca acctatgaca ttttatcact tataatttcc ac

#accatgga   8880

taataagcac ttactatgca ctactccagg tggatgtcaa atttacgtta at

#agagttta   8940

atatcacaat acaacttata tctgagaatt ggaacttgtg tgtaagtaga gt

#aactttgt   9000

aatgtacaaa tgtgagttgg tagtctggtg actggagaga ttttgagact ag

#atcttggg   9060

aaagctttta gtattgattc ttctggctac ccacatgacc ttggagaagt aa

#cttaatgg   9120

ctgagcttta gtttcctcat atgtacaaca agggtaatat ttagaagaaa ta

#agctaaaa   9180

agatggttta aataacataa attatcaaaa tatttaaata aggcagtata ca

#tacacata   9240

ttatgcacac acacgcacac acacacaatc tccacatagt aggaaagaag ag

#tcaagaga   9300

atattataga aacaattccc atacatatta aagatgacag agtttatttt ga

#atgatttt   9360

taaaataatt atttagaaga tattttataa taggtgaatg tttgccacat ct

#gcatttaa   9420

ataatttaag agctgatgat gtaatagttg ccatttcaac aattatactc ag

#tttgtgaa   9480

tttagattct gtttagggta actgttgatt tttgtatttt gcccattacc ta

#tcatagta   9540

cctgaagggt ttgttggaaa cctgacttac gaatccattt cgtcaactgc aa

#taaatgta   9600

agctgggtcc caccggctca accaaacggt ctagtcttct actatgtttc ac

#tgatctta   9660

cagcagactc ctcgccatgt gagaccacct cttgttacat atgagagaag ca

#tatatttt   9720

gataatctgg aaaaatacac tgattatata ttaaaaatta ctccatcaac ag

#aaaaggga   9780

ttctctgata cctatactgc ccagctatac atcaagactg aagaagatgg ta

#ggctagac   9840

ccttttattg tctgttaagc agattgttgt tcttttcatt tacattgctt tc

#tgatagga   9900

aatagtcttc aattatattg attctgtttg atctcaagta attagccttt ca

#ataaacac   9960

agtgtttctt aaaataatct gctaagaaaa tcaaatccca ttatgattga at

#cctctttt  10020

tttaatgctg attcactttt gtttcattta atattctctt tttcttttat ag

#tcccagaa  10080

acttcaccaa taatcaacac ttttaaaaac ctttcctcta cctcagttct ct

#tatcatgg  10140

gatcccccag taaagccaaa tggtgcaata ataagttatg atttaacttt ac

#aaggacca  10200

aatgaaaatt attctttcat tacttctgat aattacataa tattggaaga gc

#tttcacca  10260

tttacattat atagcttttt tgctgccgca agaactagaa aaggacttgg tc

#cttccagt  10320

attcttttct tttacacaga tgagtcaggt aagccagaat ccacatttct tc

#aaacaatt  10380

tcactgttgc agcgcctgct ctctcttttt aaggaacagc atggaatatg aa

#aggatatc  10440

tgattgtcta tttgtaacag ccttaccatt atatttactt tgttgatttt tt

#ttttgcaa  10500

tttgagcttc agaatttcct gttctgttta aagctacttt ggaactactc tg

#tccaaata  10560

caaattataa ttaattatga tatttgtttc tgaaatttaa atatgatcat tt

#tataaatc  10620

tttttaaact agtgtcttca agaaagtaag tcacggtgct atttttatgt ta

#aaagtttt  10680

atgaatgtaa gtttcttcat gtgttttcct acagtgccgt tagcacctcc ac

#aaaatttg  10740

actttaatca actgtacttc agactttgta tggctgaaat ggagcccaag tc

#ctcttcca  10800

ggtggtattg ttaaagtata tagttttaaa attcatgaac atgaaactga ca

#ctatatat  10860

tataaggtag gttgattata acagtatatg tttattttta aaaatcagaa at

#tgaattaa  10920

aatcttttga catataggag gaaaatggac tactaaatta aacaatgact at

#ttttttaa  10980

acttctttat ttcctacaat ttaaggatgc ttatggaaaa cacaagcaag cg

#tttgacag  11040

gtatataagc tgaatacttc atagagcaat gtacttagat ttgtaacttc ca

#gatatcta  11100

caatttaaga aacagttgca tcattttgtt aatgctggaa agtgtatagt ac

#ttttttcc  11160

tgacttacaa atataaaatg tatttctatc tattgttaac agcagcacca ag

#ggaatctt  11220

tttaaccttt tagaaaggta ttcatcttta ttctggactt ctggtcatct tt

#ccagatag  11280

catatgatcc tacactaatt ggtcttttac gatatatcct tatttttttt tt

#tattttca  11340

agagtaattc atttgcaaca ctaaccacat tttccctcct ccatttttgg aa

#ttcagaat  11400

tagtgaaaaa tatccacaga actgatgcaa caaagagtct caaatatatg tc

#tgtgattt  11460

ctagcattta attgccaaaa tgtaattaac aagcatttat ttaagaaaag tt

#tcttattt  11520

ttttccccaa aggcaaatga agtcctggaa tgttcttatt tagtttacag ca

#agaagagt  11580

gcaaaaaatc tgcagtaaat attttactca atattatgag tattacaatt ta

#tgactatg  11640

gtaaatcatt gttatagcat atgtagttta caaattgaat agtaaaagtc aa

#aagcaggc  11700

attaacttta tgtcatcggg aacaatgact ttctttctgg aaaccaagat at

#tactttaa  11760

aacttgatag tctgagtata atttgaatcc tattactcca taaatgtgaa at

#ttgtttcc  11820

cagaggtgtg aaataacatt aaatgacatg aagcctcttg ccctttaata tc

#tatccctg  11880

gtttaatctt aacattattc cattttttat ttgctttgtc tgtatgggtc ac

#tgggagat  11940

agatatcaaa aggaaaaaag aatcattttc tcagagtaat cgcattccta gg

#ataattgt  12000

gtacgtgtgt tagagtgtgg ttgtctatat atggatcttg tctcctcaga at

#ggtgatct  12060

gtaacatagg ctctcttagc atagcggtga agcaagggct ctgactccaa at

#tacctggc  12120

tcagattctg cctttgagac ttactgtgct tcagtaggga cattgcttac ct

#cttaatgc  12180

aaaatgggag ttacaaagat gtgtacattc gaaattgagg attaaaaagg aa

#agtctcca  12240

tagagcattt tgaacaattc caagcatgtg ataaatatgt tagctattgt tg

#ctgtaatt  12300

gtacacagtt tttaaaagaa caaaaaaact gtccaacatt gtaatagcac ta

#agcatgaa  12360

atgacaatat gccattatgt gaacatgaga ataacttgta ttctaagatt tg

#taaacagg  12420

ttttctcaat agaagcacat ctttaatatt tcagaagtag caaaaatacc at

#ctttatac  12480

cattaagtat tcaatacatc atttgggatg ggcagtagtt ttgtgtttta aa

#atctactg  12540

cgtcatgtta ctccttttta catctatttt ctctttcatc aattttcata at

#cttatttg  12600

ctttcaaatt cctttaaatg tactctcatg ccatcttttt ccctgtcttg gc

#atctagtt  12660

actatatctg ctttcctttt tctatttcgc tttctctcct agtgtgttct at

#tttccttt  12720

ctctttcatc taccctgata tcctgacagt atcaatttat attattttct ct

#gtttttct  12780

attctttttc ttcttttaaa ttatgtgtgc atttgtggag gtagaataat gc

#ttgaacca  12840

cttcaaatgt tactgctatc ccagtcaatc cactgtggtc cacagataat aa

#aatcaata  12900

ggtgacttag gtcactcaca catactgaaa gaaattattt atttagtaca aa

#gttctatt  12960

aaaatatgtt tgtaagtatt catcactcat gtttctcttt ttgacaacat at

#tcttgtgt  13020

aaatctgttc actatcccat aaactattct cttattatta tgccctcttg gg

#ttcagttg  13080

tttctctggt ttttagccct tcctaaccaa aatcataatt tgcttgtttt gt

#gtttaatt  13140

ttttctcatt cagaaattgt agatttctct agttaatata aaaattcctt ga

#tggcaggg  13200

accatgcctt acatgtgtct ataatctcca gattatctaa tggcatgttt tg

#taaatagt  13260

aagcaggaaa gaatgactga aataaagaga ttcagtaagc ccctaaattc ag

#tgaatttc  13320

tagagattat tttaaaatag gattctaatt gtaaattccc caagaattaa ta

#ttcttgtt  13380

aaaatttctg ttactgtgat gtttgataaa tgatcaacat ggtttatatt tt

#gtcagata  13440

taatgtaaga ttcctacatt tatatcacat aggagattat cttctctttc ca

#tgaggata  13500

gctgattaat cttagctgct ttcttggtta ggacaattat ctttgaatga aa

#actttgta  13560

cttaatgata atttttttct atgagaaagc atattcctcc ttgggcaact at

#gatactct  13620

tttgttcctt ttctcatatc tctaaaaaca gtgtcaaatt agaaatagag ga

#atcggctg  13680

ggaaaactcc caatttaagc ttcatggaag cagatatttt aaaattatta tt

#ttaaaata  13740

ataataattt attattatta tatataataa tgttattatt atttattact gt

#tttgccag  13800

tgtctacata cagtagctga agaataaata aatttacaca ggaatgctgt gg

#gtattaaa  13860

aatgaattta gataagttca gaaaactcaa gtatctctga ccatgcacaa gt

#tggattta  13920

aattgcagac tgtaattatg caaattaaaa aaaatgagta tataattcca ag

#tgaaaatc  13980

atgaaaataa aacactctag ttttttaaaa aggcaattat acgccaggtg ca

#gtggctca  14040

cgcctgtaat cccagcactt tgggaggccg aggtgggcag atcacctcag gt

#caggagtt  14100

caagaccagc ctggccaaca tggctaaact ctgtctctac taaaaactac aa

#atattagc  14160

tgggtgtggt ggcacatgcc tgtaatccca gctacttggg aggctgaggc ag

#ggagaatg  14220

gcttgaacct gggagtccac ctcccactgc actccaacct gggcaacaaa at

#gagactct  14280

gtcaagaaaa aaaaaaagtc taaaaaaggc aattatgagg ttcttcaggg aa

#aagaaggt  14340

gcccaattca tccttgtatc ataaactgag cacactctat ggcacaaaat aa

#atgctaat  14400

atttgtttta ttataattta aaatatccat gcttattaaa ctataggtta aa

#tataaaag  14460

gaataacttc aatgaaaata ttccattgat gaacaatttt ttgacagtgc at

#taactaat  14520

aacttttttt ctgtttttca gaatatatca ggatttaaaa ctgaagccaa ac

#ttgttgga  14580

ctggaaccag tcagcaccta ctctatccgt gtatctgcgt tcaccaaagt tg

#gaaatggc  14640

aatcaattta gtaatgtagt aaaattcaca acccaagaat caggttagat ac

#agtttttg  14700

agcctaaaat gtttcttttt atatttaaca cctttctttt ccttttctta gt

#ttatatga  14760

taaagtatca ttacttaaga gtctactcaa agggaaattg catttcagtg ct

#ttacgttt  14820

agtcttggtc ttgtgtgaaa tcatatgctg tatgtgtgtt tatacatata tt

#ttcacaca  14880

tggtttttcc ttttgaacag aggaagttga aataaaatag tagtttggga ac

#aaaatagc  14940

cttctagata tctgtgaaaa ttacctaatt cttagaactc tttgagacag ct

#ggggaaaa  15000

agggggaaat gaactagcag tcacttttaa cgggctgatt tatattttta at

#gaaacaat  15060

atctataatt ttcttttaag aagattagtt gtgacatttg gagagcatga gt

#cattgcat  15120

aagcccccta tgttcccatc atcccatctt taccatgtgg cggacactga aa

#tatcattg  15180

gtctaattca tcaacagctt acctgctgtg tcacacatgt agtatacatg ac

#atatcttg  15240

cctttgtgtg cacactgaat agtttttatt taggacctat ttaatgatgg ct

#tagaaatg  15300

tacttttcct tttctcaact gcaccatacc tttaaaagca cctcttctta at

#tttttttt  15360

tgtttacttc tgtcaatgtt tattgaatga gcaaaagatc ccgttctagt ca

#tttcttct  15420

tatcagctct ggatgcactt cctggtatgt tagtgaatct ttaaatcgag at

#tgtagacc  15480

actgactact aaattaatca tttctgcata aatttatggc tacctgacac tg

#tttttcgt  15540

gcatttctgt aacaaatgca aaataaatag catttataat ggataaaagt ac

#atgctgtg  15600

aagtcatttt ctggatttga atttgagccc catgacctac tagttgtata at

#cttggcaa  15660

aggctcatga ctctgtgagc ctctgtaacc ttaactgaaa agaagcacat at

#tagcagta  15720

gccatctcat aatgttgttg tcaaaaatat ttggaaagat ccacataaag ca

#ctttatag  15780

agtgttgtac acacagtaaa tgcccacttc atagagtgtt ggacacacag ta

#aatgaccc  15840

ctgaatatta ctgttgcccc cattcccatg ttacagatga agaagccatg at

#tgagctag  15900

attagatgaa aaggaccttg aaaaaattag tgaagaacct aactagaaca tt

#ggccttct  15960

gacttctagt gaagagtgga catgactgca ggaaatgcat gttgtgaatg ag

#tgatagaa  16020

tataaaaatg ttcaacccat aaataaaaaa atattttaat aatatttgta ca

#tgaggaca  16080

ataagaatca gggctaatct tgtagaaagt gctctgtaaa cccataaata tt

#ttttatca  16140

gtaagataaa attgtacccc aacattctat actctgatta tttaaataaa ta

#aaattttc  16200

acctttaagt gttttaatat caatggttaa tttttttttg aggttcaaaa aa

#tcaggaaa  16260

atggatattc acaaaatctg gatttagaaa ctaaagttca gcaaattgtc aa

#ctatctta  16320

tgttaactta ttttataaaa atgttcttat atgattctga aaacaaggaa gt

#gaatagtt  16380

aatagcattt aattgccaga tcccttgatc agccagaaat tatctttaaa aa

#atttttta  16440

atgccacata ttccctaaat attctccttt agtactggtg tctttatctt ac

#agaggaag  16500

aaaagtttat aacagctcag tttagaccca ggtagagcgg tgtaggcaga tc

#agggatca  16560

cctgagtatt ctttaaagca ctatgttttg cataatggca gcaagttatt tt

#ctttcaat  16620

tttcattgtt tgtaatccac aaattgactg tgtcccaatt tttcttctac ca

#ttatcttt  16680

tactgtgacc agaaaagtta ttctactaat gccaccatta ggggacattg gc

#taattgga  16740

catttctgtg ggaagtaacc agtttctcta atgtgcagtc actttggtgg gc

#taggatat  16800

tgttctttga ccaggcctac cagatataga ggacctctga gaagctgggt ta

#gtttcaag  16860

taaattcaga gaagctctag aaaataagac tgagactcct taaatcttcc tt

#ccaatgat  16920

gtctacaaaa ggtacttaaa aatgaaatcc tcaagattct tccaaagaag cc

#atcccggt  16980

aaaaaccagt acctttaaat tagttagggg tttccaagta ctgtgaagcc ca

#gatttgtc  17040

acaacaggga ggcacctgca tactatgttt tgcataaaaa tgttccataa ta

#aagtattg  17100

ctaagatttt tcctttccaa ttaagagagc agttatcaaa cactgcctgg gn

#nnnnnnnn  17160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaagagagc ag

#ttatcaaa  17220

cactgcctgg gcctgggctt gaggctacac atttgcttct gagcttttga gg

#atgtgatt  17280

ggtgcttcga actggaagat atttagtgac tggttcaata ctgtaatgat ta

#atacaata  17340

gcataaaaag caagtcaaca gccttttgat tctgtctatg ttaatgactt tt

#taagcaca  17400

cattgaaaat ttgatatatt aaatattttt ctagttctaa acacagatgt at

#ctagtgat  17460

cacgtaattc aatcaattat ctacttacat atgtatacac tttaactttg gg

#catatgtt  17520

tatctcttaa gttccagatg tcgtgcagaa tatgcagtgc atggcaacta gc

#tggcagtc  17580

agttttagtg aaatgggatc cacccaaaaa ggcaaatgga ataataacgc ag

#tatatggt  17640

aacagttgaa aggaattcta caaaagtttc tccccaagat cacatgtaca ct

#ttcataaa  17700

gcttcttgcc aatacctcat atgtctttaa agtaagagct tcaacctcag ct

#ggtgaagg  17760

tgatgaaagc acatgccatg tcagcacact acctgaaaca ggtaactaac gt

#gaaacagg  17820

taactaacat gaaaccttta actatttggg gattgtgtca ataccacctg ca

#atctttat  17880

agcatactta tctaaacata caaagcacat attaaaaaat acaacacagg ct

#ttttatcc  17940

cacgtgttgc ttgagtgcca gctgtgtact acattgaccc ttctccaaaa ca

#ttgggaga  18000

ttgaagggag gaaaaaaaga gagatgatcc tctttactgt atttccacaa at

#ataaaacc  18060

cccacctaat gaattatgct ttattgtgat ttaaaagaag aaataaacat gt

#aaaccttt  18120

catgtatatc tctttttagt cttacttgtt tttatggaat tctagatgtt tt

#cctgaact  18180

atatggttgc agtatcagac tcattttcat ctattttctc ccctttatac ca

#gcctttat  18240

ctttcatgtt atttgaataa aatatccggg tcgttaagct ttagtccaca ag

#acgaaatt  18300

ctcaccttcc ctagcagtgc tctgtcctgt atcataatat ccttcatcct at

#tttcttcc  18360

atattctacc tgcttatata aattaaaacc tgtttctttc ctgataacac ca

#cttcactg  18420

tagatattgg caataattgt taacttctgg cacatccaga ccctttatct tg

#gaaacgtc  18480

tttcaagctg tcttgaggct gtaaacctag aacatcaaga catagtctgc ct

#tctctctg  18540

atttcagcat ctaactccac atcctttcct tctcattctt ccagtgcaac at

#tttttcag  18600

actacggtgt ttccctttcc aggatggaat agttacattt caacaacacc at

#ctctttgc  18660

tccttagatc tcataccatg tcattgtgac ttaccctcca ggaagcttcc tc

#actctgag  18720

aaggccccat tatttgtttt ttccaagatg ctgactggta aatatttcta gg

#aaaaaata  18780

gaaatgattc tactttgttt gtctataaat tcatcgtcct taattgtccc ag

#ctgctcca  18840

aaattttcta tgtatccctt gtttattctt cataggaaat atgttcatag ga

#atactctc  18900

tattccatat gaaaattgtt ctctttctga acctagtctg ttcccccatc at

#ccatattt  18960

attgttattt tactaataat atcaaatata ttgataggcc ctccttccat ca

#aaatttat  19020

ccatgtcttt atttatgccc tccagatatc ttctcttagg aagtccttgc ct

#tcctcttt  19080

cagggatcta gctgttcatt ttcattttaa tcttatgtct tctctaggat at

#taccccat  19140

caatttattc taatatctcc tgcatttatc ctctttctct ttttcttgca ca

#ttcaccca  19200

aattgttgaa aaatcccaac tgaagaccta gttggagtat caactccaaa ta

#tatatgaa  19260

atggaatttg tgttacatga aatcactgtc tttttcttag ttttctatgc ct

#gttcttga  19320

caatccattg gaccccaagc ctcatagttt tatacaattc cttactccat ct

#cttctaca  19380

tacaatcagg tcttatcaat tcaatttcca tcagggctct gcaatttgcc cc

#ttctccac  19440

cttggccacc accattgtat attagaggga ccttgttgct tcctgtaata ac

#atcttaac  19500

tagccttatc acccacacta ctttagccta cccataagtc tcatctttcc tc

#ctactcac  19560

ttaattgaat tggttctaac atacaattag accatttata cattggcagg tg

#aaatgtaa  19620

tatctgaaca ataaagttta gacgtgtcaa gttgggtacc tgaagcacag aa

#gtaataat  19680

gaaaggcacc atgtgtagga gatgggttaa aatactccaa atattttgct ca

#ttctcatt  19740

gtctagaaaa tatcccagaa tccatcgcta attagaattt ggcttctctc ta

#gcttttta  19800

ttcttatatc cttttattgt atcttctcat atagctgatg tctccagcca aa

#cttcattt  19860

atttagcatg ctatttcact atttcagtat tttaactcag aaaacattta tt

#aaacatct  19920

agtatgaact aataattgac tagattctct tctttaaaca tatccaacca tt

#tttcatca  19980

tcagattgct ttgcttcctt taactgtatt attattttcc ctttctatga ca

#cataaaat  20040

tttattcatt tttaaaagcc cagcttaaat gtgccttctt tattaaagcc tt

#taatggca  20100

tttctggact tcatcccaga cttgctgact catagcctca atgagtgaag ct

#taagaatc  20160

catgcactta agaatctctc taggtaattc cgatattctg tatgtattgg ag

#agtactgg  20220

agtagattac caggaacttt tgaggacagg caaagagtgt cagaaaggtc ca

#tcaagggg  20280

tgacaggtgt tttcccagtg ggtggccagc acatcattgc tatgtggagt ct

#gtgggaag  20340

aaaaaatgtc aaaatgtcaa aatccaggta ggtggtctgc atcagggtgg tc

#tagcacca  20400

ggtataatgg tcttgatcat tgggcaggag ctgagtcttt gagaactggc aa

#ataaaatt  20460

acaggacaac atatctgaaa taaatgagag attcaggaac aaggcaggag at

#actaggtt  20520

ggtgcaaaag taactgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  20580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  20640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  20700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  20760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  20820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  20880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  20940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  21960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  22020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  22080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  22140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  22200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  22260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  22320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  22380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  22440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tt

#tggtttta  22500

aaaacttggt cttttgtgga agataatttt ataatttggg tttatccaga gc

#tcttccaa  22560

gctccataat ttagaatcaa aagagaaaaa taaggtactt ccctcagatg ca

#aaaattta  22620

atttagggaa agtaaaatgt gtatttctgg tttttagggg tgttcttttc tg

#cagtgatt  22680

tctttattag ctttttgtcc agtggaagat atcaggcata tgcagtgatc ca

#ctggaaat  22740

ccactgagct tgcaaatata ttaatgctac aatatgattg acttggcatg ta

#ttcaattt  22800

aattatcatc atcatcatca tcatcatttt agagacaata tcgcactatg tc

#accgaggc  22860

tggagtgcag tggctttatc tcagctcact gtagccttaa cctcctgggc tc

#aagtgatc  22920

cttctacctc agcctcctga gtagctagga ctacatgttt gcaccaccat ga

#ccagctta  22980

ttttttgttt gtttgtttgt ttgagacagg gtctcattct cttgcccagg ct

#ggagtgaa  23040

gtggcgctat cttgactcac tgcagcctcc acctctcagg ttcaagcaat tc

#tcgtgcct  23100

caggactccc aagtagctga gatcataggt gtgcaccacc acacctggct aa

#tttttgta  23160

tttttagtag agacagggtt tcaccatgtg ggccagtctg ggtatcgaac tc

#ctgacttc  23220

atgtgatcta cctgcctcgg cctcccaaaa tgctggtatt acaggcgtga gc

#caccgctc  23280

ccagcctgcc cagctaattt tttatttatt tttgtagaga tagtctcact at

#gttgccca  23340

ggctggtctc aaactcctgg ttcaagcaat ccttctgctt cagcctccca aa

#gtgttggg  23400

attacaggca tgagccacac acccaatcta gcttatttgt taaatacatt ac

#ttatatat  23460

tttataagaa tttataaaat tcttatatac catttaatag attgaatgtg gg

#cagtaaaa  23520

ctgctgccct tctatggcta caaattagtg cactaaatca aaagttcact tt

#tcctttgt  23580

atcctactta catagctttc ctcatccatc tcctgaatta agatttgaaa ta

#aaggatgt  23640

aggaaagttg catgattctg attgctctca agcaagtgaa taaaaacatt ca

#acttaccg  23700

gtgggtaatc actagaagca caaaagacat tatagttgcc tatcataaat ca

#gagggaaa  23760

taactattag tatatctaat tgaaattcag gtgttttata cagtatcttt ta

#tatagact  23820

taattattaa atataatatt tttcttcagt gtgaacatca ggtgaggaat ct

#ctttttac  23880

acttcttgta gtgcagtgga cagttgacca atatatactt atttactgct tg

#ctatgttc  23940

aggttctagg tgtggggttc aagacccaag tttgagtgcc aggtggcatt gg

#ggatcttt  24000

gccctgcaca tccaaataac tccattatta taataaaaac atatttaata tg

#tataaaac  24060

aaacacaaac ctacataata tgttggtcaa ttnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  24120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  24180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  24240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  24300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  24360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  24420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  24480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  24540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  24600

nnnnnnnnnt ttatcagtat gctatgagaa aaatggcaat tgtacatatc aa

#agaacatt  24660

tcctctttta ttggaaatat tcttggatgg ttaggttgag atcagaagag at

#tatgggtt  24720

gactaaagca ctcatggtaa gctgccctcc gacaccctca ctattcatga aa

#atagtgag  24780

aatagtagtt agaggagaat aaataggaat ttcaagatac agcaagagaa aa

#cacatagt  24840

ggagaaagga atgcagtaca aggggtcagg ctgagaccaa agcttgacta ca

#gaggaggg  24900

tattttatta gaaggagtgt aagcaggaag gttgaataac tgaagtggac cc

#ctacttac  24960

tctgctctta gtttgatgtg actgtcccag aagtttgaat ctgttataat ag

#aaaatcag  25020

gaacttgtgc taaatttaga gaaggaaaca acataagtaa ttctaaaaac ag

#tgattgtt  25080

tttggccatt ttcctgaaca ctgcagaaat ctctttagat ggaggatttg tt

#cattacat  25140

atttactgag cctcctaagt aatacagaat aaaatctcca gtcatttcaa tg

#gcccataa  25200

agcccttctt ctcaatccag ttatctgtca cttcccttat cttgaaattt at

#gctcaaat  25260

gctacctctt caaaaaggat ttctgactaa tttgcccttg aacgtccttc ct

#cccagcta  25320

gaattttcca tactcctttc ctgctttact tttctctttt ggatatatta ta

#tgtaccat  25380

tatctgtggg tctgttctca catgctgcat ggggacagag atttttcttg gt

#tcaaacct  25440

atttccaatg tccagaaaag tatctggcat acgtgataat aagtatatta ta

#aatgaatg  25500

aatcaatcaa tgcacaggcc aagaagagtg ataggcatag agcaaacacc aa

#gtatgcat  25560

atgctgggtg tcttaagtag aaacttgcag tcacaaaaca atttttaaac ag

#ttatgtat  25620

taaaacatat gagaaaggca tgtcttgatg aatnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  25680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  25740

nnngtttcac catgttggcc aggctggtct tgaactcctg agctcaggca at

#gtgcccac  25800

ctcggcctcc caagtgctgg gattacagac atgagccact gcacctggcc tt

#tttttttt  25860

tttttttttt ctttttggta tggatgtacc acagttccct taaccattca ct

#agttgaag  25920

gacatgtgag ttgtgtccat tttgtggcta ttagaaataa agcttctata aa

#cacacgtg  25980

cacaattttt gtaggaacat aaattttcct atgtctagga tatgcactga gg

#agcacatt  26040

gctgagtcat atggtagttg catgttaagt ttttttaaga aactgacaaa ct

#gttttcca  26100

aaggaattgt acccctttaa attcacacca acaatgtatg agccatccag gt

#tcacacat  26160

aggtgtataa tgatgtatca ttctggtttt gtgtttccct gatggctaac gt

#tcaatagc  26220

taattgaata tattttttat gtacttatgt accatctgta tatcctcttc ta

#tgaaatgg  26280

ctgttcatta cttttgccca tttcctcatt ggattgtttt gttttattgt tg

#agtttggg  26340

gacattttta atacattcta gctacttgtt tttgttagat atatggtttg ca

#aatgattt  26400

ttgccaggct gtagcttgtc ttttcttttc tttcttttct ttttttgaga cg

#gagtctcg  26460

ctctgtcgcc cggggtggag tgcagtggcg caatctcagc tcaccggaac ct

#ccgcctcc  26520

cgggttcaag caattctcct gccccagcct cccgagtagc tgggactaca ga

#cgcgtgcc  26580

accatgcccg gctaattttt tgtattttta gtagaggcag agcttcgcag tg

#ttacccag  26640

gatggtctca atctcctgat ttcgtgatcc gcccacctcg gcctcccaaa gt

#gctgggat  26700

tacaggcgtc agccatcgcg cccggcctgt agtgtgcctt ttcatcctgt ta

#gtagggtc  26760

ttttacaaag caaaactttt taattttgat gaagtcctat ttatcaattt tt

#tcttttat  26820

ggattgtgtc tatggatgtc taattgctct agcaccactt gatgaaagag ct

#gtctctcc  26880

tccattgaat tgctttttct tttgaagctt agtatgttgc ttgaaactat gc

#ttgttaat  26940

actgtatact gaaaacgtac aaagaataat gttccaattt aagttagatt ta

#agttaatg  27000

atgttcatta ataaggactc ttcagatata aatattccag aatttctctt ag

#ctttctaa  27060

tcaaaacaac catcagtgaa tattacctta ctttggaagg tatagatata ca

#tttgaatt  27120

aaatttagtt tttccaaata acccctaatt tgagaaatat attctatctt ga

#aactaaaa  27180

taatttaata caactttatt ttcttccctc ccctcccctc tcctgtccac at

#tttgtaaa  27240

atctggtcct gaataagtca caatataaaa ataaatgtac acttaacttc ca

#cttcctcc  27300

aaccacaggc tactttctgt tcctcaacct tgggaacaag gtgaaaaaca gt

#aagcaatt  27360

tgggcagggc attgccaaaa caagattcaa gcagccacat gtggacacct ct

#taaaagaa  27420

tttggggaaa cgagaccaaa gaagtcaggt ttgattttta gtgacaataa ca

#aacatgaa  27480

gtgactcttc ccaagtaaga gtgccactgg gatgtggcct ggccacatgc tt

#acctatgc  27540

tatacttccc aggaaaccct gatgctctgc tccaggagag acctttatcc tt

#tggaggtt  27600

cagtgtcttg ggagctcttt gattttgtca aagagatgag cagagattcc ct

#gtgggtat  27660

tttaaggctt ggtgtcaagg tatttttctg acactgctga gcaaagtcca tg

#tatcaaat  27720

gatctgtttc tagtttgttt aaattcttca catcacttgt agacctaaca tg

#gcaaagct  27780

tcattattta atcataataa cacctactac ccatactaac ttatgattta tt

#ttctgtgc  27840

ctggaaatag tctctgtgtt taacaataat acctggatgc aaaacaatcc ac

#tgttatat  27900

ggccacaaaa tattaatgat cttctgaagg ccaagaaaac attttaacta ta

#gttcttgc  27960

acagaaattc acacccagaa tccccaaaat taaaaaaaat ttggacaaca ca

#aataatag  28020

tttaagatac acatacatac aacacagata ctcttacaca taacatcttt ta

#cggaaatg  28080

tgtttagtga aactgttcat ttgttgacag ccacagaagt catattttgc ta

#aatagctg  28140

ctccagctgt ttttttcttt ggaaaatgta tcactatagg ataccctgtt ta

#ttgcataa  28200

gataaaagaa aaatatgttg tgataaccaa aaagttttaa gggctttcaa gt

#tatgtaaa  28260

aatggaccta tggacatggt taattgtcct caggatgcaa aattggagct ga

#aatagtat  28320

atcaaacaat tgcaaaaagt gtactgcagc tatctcttgg gtcaaatctg gt

#acccagaa  28380

atggagaaaa gcctcaagaa acattgctgg ttggccctct gccacttgac tg

#tatgatct  28440

gatcacatgt aagtttcaca aacgattcat atttctctgc tagtttgacg tt

#gagaattt  28500

gctcataaac ctccctaatt ttatcttctt ggtcctttga gaaacacata gt

#atcccaac  28560

ttgtcagaga ggaaatttga gctggtcctt ctttatccag gagagacctg aa

#aaattagg  28620

tggtgtgagt actgcagagt gaggctgatt ttccaaagca ctaactttgt tc

#tgattaag  28680

aacaatttac aatggtctcc actgctggta atgattatct tcttttacgt tc

#tgaaaaat  28740

ctgctctggc tgggaaggtg ctgctcactg ccaggtggga tgggntgcca ta

#cctttgga  28800

aaaccatggc ttagcagtgc cacctccatc tccatggttc actccagggt ca

#cccaccgg  28860

tcatgccatg ctgttgaggg gcagagacct ggagcagaca ctgatatgac tg

#cctgcaca  28920

gccactggct tctcggtggt attcaaacgc caagccattt tcccatactc tt

#tgagtttg  28980

aggaactttt tggagattgc ttgagattct ctgcgtagaa aatcatgcca tt

#tgtaaaaa  29040

gggtcagttt tgcttcttcc tttctcatct gtatgccttt tgttttttct gt

#cttactgc  29100

actgactaga aatttagctc tatgttgaat aagagcaatg aaagaggaca cc

#cttgcctt  29160

gttcctgatc ttgagagaaa gcattcaatt cttttaccat tgtttgtgat gt

#tagctgta  29220

ggttcctctt tatcgagttg aggaaattac cctttacttg tatttttctg ag

#aattttta  29280

tcttaaatgg gggttaaatt ttgtcaaatg cttttttttg cattgattga ta

#gtattctt  29340

gcaatttttc atctttgctt gttaataagg tggattatgt tgattgatct tc

#taatattg  29400

aaccagcctt gggattctta cactgcttgg ccatgatatg catattccta cc

#ccacttgg  29460

cattgatgta tgtgtatata taactgattt ctatttgatg atattttgtt aa

#ggattttt  29520

gcatctatat ttatgaaaaa tactggtcag tagtttgctt ttttgcactg cc

#tttatcta  29580

gttactgtat aagagtaata ctagctgtgt gaaatgaatc tcttctaatt tc

#taaaacag  29640

attgtgtgga aatggtattc atttatcttt aaacaattgg gagagttctc ca

#gtgaaact  29700

atctgaactt agagatgtct ttttggagaa ttttaaaatt acatttttat gc

#tctcagga  29760

tgcaaagttt gagctgaaat atactatcaa acaagttaga aaaagcaaca ct

#tctcgata  29820

attcatagat tgcaaaggaa atcttaagat aatttttaaa atacatcaaa ct

#gaatatgc  29880

taaaatatat tgaattgaaa tgaaaattca acatatcaaa atttgtgaga ct

#cagtgaaa  29940

agaaagaaat ttgtagcact aagtgaatat atttaaaaag agaaaataga cc

#aataatct  30000

aaattccctc cacaaaagaa agcctagaga tagaaaaggc agagaatcca aa

#ccatgcag  30060

aaggcaggga ataataaaat gcagaaatca atgaaattga aaacagaaaa ac

#agtaggaa  30120

aaatcaatga aatgaaaagc ttgttctttg aaaaaataaa taaaattgac aa

#atctctag  30180

caagcctgac aaagaaaaaa agagaaaatt caagaatgaa acaagtgcag ac

#actgcaga  30240

cataaaaaaa aaataagaga atactacaaa cagctctcac agttaaattt ta

#tatatgag  30300

atgaaatgta ctgattcttc aggaaacaca cctactacac tatactccct aa

#tatgaaat  30360

aggtaatttg aatattttga acagttgaat aaaattaata ctataagtat ta

#aagacatt  30420

aagtgtataa tggtttcttt gtttgggctg ctataatacc agaaactgga tg

#accgataa  30480

acaacaaaac tttatttctt acagtctgga agctggaaag tccaacatca gg

#gcaccagc  30540

agattcagtg cttggtgagg gcccattttc gtgttcatag atggttcctt ct

#tgttgtgt  30600

cctcatatgg tgaaagggat gaggcagcac tctgcagcct aatcccactc gt

#gattactg  30660

ccctaatccc actcgtgagg gcagagccct catgagctaa tcccactcat aa

#gggcccta  30720

atcccactcg tgagggcaga gccctcatga gctaatcacc tcccaaaggc ct

#tatatcct  30780

aatgtcatca cactgctgtt taggtttcaa catatgaatt ttggggtgat gc

#aaatattc  30840

agaccaaagc agttttcttt tataccactc tagtagagaa agggaggagt ac

#gtctgtta  30900

ttgccaggta ggggtagaaa tccgggtttc caacttgggc tttgttatac cc

#aaggagag  30960

gatttctcct tgctcctggg ttagcttggg atttttggct ccctactaag tc

#tccactgg  31020

gatcaccctg gttgggagga gtagtgatac cttttcactg atgtccacat gt

#tttccatt  31080

gacattatgg tggaagggtc ttattactan nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  31140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  31200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  31260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  31320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  31380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  31440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  31500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  31560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  31620

nnnnnnnnnn nnnnnnnnnn nnnnnnncat ccatgggtat attttgaata tc

#aagcaggg  31680

atacttttta tccatctatt caagatcaag ttattaaaca catattaagt gc

#tgtggtag  31740

aaagtattgg gatgactgta atgaaatgaa tatatttctt gtgctattaa ag

#tttagaat  31800

tatataattt tagatcttca ttgaatctca tagataactt catttagtca tt

#tcattttg  31860

cagacatagg aaatgaagca cacaactgaa gtgtttgttg agttttctac aa

#ttaattat  31920

ttgcaaaact attactagtc cagaattctt tctactatat tgtctcccct ac

#cttagaaa  31980

ttcaatacat cattgtgttc attggaatta caggagtttt cttccattat tt

#cacaatgt  32040

ctaagtacag acattatgaa gtagggaaat ttactttcat tataaaactt tc

#ttcattaa  32100

catgtataga tacatttata atgtgagtat atacatactt ttgtccaaag tg

#gatttaaa  32160

attcaaaaaa aactaaattt ctatgatcaa atccatgctt agtctataaa ac

#taaaaata  32220

ttgtgagtta acgtaataag atctgtaaaa tactgaggcc attatgggaa at

#gtttaaag  32280

ttcctacatt catatcacat tttttatctt ggatcagttc caaaagtgta at

#gtttgcta  32340

ttttgaaatt atcttaggta tcaaattcca acttataaat ttaaaagttc tt

#taaatgta  32400

attcctttta taaaaagtga atttgggtta ctctgcataa ttctccttga cc

#ccactgat  32460

gctttaatat ctctcattaa gtggactcca ggcagccact ctttgcttta tc

#caagctcc  32520

agctaaggcc agctgttctt tgagcagtgt ttttattaac ttatttagga ac

#tggtgcat  32580

atcttattac cctattttcc attcctgtcc tatgcagctg tagttgatac tt

#ttcataac  32640

agcctttaca tatcaacctc ctcccctatt ttttttctta ttctctttta ct

#tccctttt  32700

taagtaaatt aagcatgtgt gtgcatgcaa aagccctctt ctttctttct gg

#taaccata  32760

tgacgtgaaa gcttcaggaa tgtgcctgtt gtttgtctga gattataaac gt

#catggaaa  32820

aacttttact aatgatgtaa acattcagaa atgtagaata catgaatttt aa

#taatagca  32880

aaatttcttc aatgttgcat ttaagaaatt aatttagacc taatttaaaa tc

#aatgcaat  32940

gtaaatacaa agaaaaggat ttgaacagat agaagactgt acaaaatact ac

#taacctca  33000

gcttactgaa tttcaaatat tacaagtttc atggcatatg aaaatacaag tt

#tgaggagg  33060

gagctatttt ataaatgtaa gacacgcata agttgcagcc actatgagat ta

#aacacatt  33120

caaaattcaa ataaggtaaa agtagcattt tttagtatat taataagtta tc

#attgcaat  33180

ttgaattttc actacctact cactacaacc ttgaataaat cacccattgc tt

#ctatgtct  33240

agataccttt ttatgcagaa ttactatttt aaaagcaact tatattagaa at

#ataataaa  33300

tattattcca tatgaattgc aataatgaaa tctatactta ttaaaagata ca

#ttaaaaat  33360

taatagccca agccaggcat ggtggctcat gcctgtaatc ccagcacttt gg

#ggaggccg  33420

aggcaaggtg gatcacttga agtcaggagt tcgagatcag cctggccagc at

#ggcaaaac  33480

cccgtctcta ctaaaaatac aaaagttann nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  33540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  33600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  33660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  33720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng at

#ctcactct  33780

gtcacacagg ctggatatgc agtggcacca tcacagctca ctgcagcttt aa

#ccttttgg  33840

gctcaagcga tcatcctgcc tcagtctccc gagtagctgg gactacaggc ac

#atgccacc  33900

acacctggct aatttttaaa atttttattg agacaagatc tcactatgtt gc

#ctaggctg  33960

gtctcaaacc actgaactca atcaatcctc ctgccttggc ctcacaaaat gc

#tgcgatta  34020

caggcatgag acactgtgac tggcctactt taatattttt taaaaatcaa ga

#tcacattt  34080

tgtaattttt aaacacacta cattaatgat atttgttgtg catgagaggt ct

#agcatttt  34140

taaactttgg acttgaaatt taaagcaaaa tttgtattta ggttgttatc aa

#agaaatgg  34200

ttaactgtgt aaaacatgtt aaaagttgtg tgtgcacctt aaaagctaaa ta

#ggatgcca  34260

tactcagaag cactattagg aactttgact gcagattaaa caggtaccaa ac

#aatagttg  34320

aaagtagttg gtgacatact tgggctaatc attgctaagg cttcctttct aa

#tatggatg  34380

tatgagaaat atagtaaagc ccatgattgt ttttctatta aaaatctaca tt

#tacaaaat  34440

attatctaga aagtatgagt gtctagtact tttaatttct atatacatgc at

#aacctgtg  34500

acttgttttg agtattattt gtacattttt atgggaaagc tttttcatgc tt

#ttaatatt  34560

ttctacttat gggaaatgta tatgcagtga catgtacaga acttgtgtac aa

#ttcaatga  34620

gttttgacaa gggcatacac ccatggaacc accattcatn nnnnnnnnnn nn

#nnnnnnnn  34680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tatattttat tgtaacttta at

#tcacattt  34740

ttctgatgat tcattatgtt ggatactttc atatgattat tggccattca ta

#tacaggca  34800

gtctttgctt tacatagtag tataggacta aaaaatgatt atagaaactg aa

#tttgtgca  34860

aagtaatcct aataatcaat gtagaaaatt atgattgttc tgtgaccttc aa

#aaattttg  34920

ttacaatatt caaaacttca agtgtcaatt ataaatgtat gtgaaaacaa aa

#aattattt  34980

agtatactaa tttaaaacat tagaaacata gagatttttt tgtataaaaa ct

#tatcaaga  35040

attttttttc tcattgttca gcttatgtta cagagagggc ctcttttcta tg

#tttcagtt  35100

aattgttata cactttcaaa gtttagatca gttttcaata ttttatcctt tg

#cactttca  35160

atattgtcaa atatcagcaa gagttctctt ggtgtgaaat ttttgttttg ct

#gtttttta  35220

cttccttcaa gacatcatcc ttcattgaat tcatcagaac cacttgcttt aa

#tttctgtc  35280

catatttgtc ttcagtaagt tgtcttggct gcatatgtaa agtttcttga ac

#agcagtgt  35340

taacattccc atggtcagct ctttgttctg aaactccgtt tatgttatat tc

#aaatttca  35400

tttccagcac tctcactttt gttgctctgc taccatcttt gttagccaat at

#gtgtcaca  35460

cgagttcatt gctgtgagac aaggaggcaa cataactaca caattttctc cc

#tgtgcata  35520

aactgaagaa caaatgcaca atgaccaatc aatgacagat tttgaacaaa gt

#gatgtcac  35580

tgattataat gtgcatctgt tatttatgta atgattttat ggatgaaaga gc

#tagaagca  35640

aagttttcat attataaaat ttttcatacc caatatatga tagtaacaaa tt

#caaactct  35700

gttttgagaa gacaggtgtt acttaactaa accatagtaa caaaaattca ag

#cacattgg  35760

aatgtgcaaa ggactgactt atctccttta gggaagtttc tgttcaagtc at

#tactgcat  35820

ttttctcctg aattgtctgt ctttttagta ctgagttata ggagttcttt at

#acattttg  35880

gatacaattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  35940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  36960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  37920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gtataaggtg taaggaaggg gt

#ccagtttc  37980

agttttctgc atatggctag ccagttttcc caacaccatt tattaaatag gg

#aatcattt  38040

ccccattgct tgtttctgtc atttttgtca aagatcagat ggttttagat gt

#gtggcttt  38100

atttctgagg cctctgttct gttccattgg tctatatatc tgtttggtac ca

#gtaccatg  38160

ctgttttggt tactgtagcc ttgtaatata gtttgaagcc aggtaccatg at

#gcctccag  38220

ctttgttctt tttgctaagg attgtctttg ctatgtgctc ttttttggtt cc

#atatgaaa  38280

tttaaagtgg tttttttcta attctgtgaa gaaagtcaat ggtagcttca ag

#ggggatag  38340

cactgaatct ataaattatg gagtctcact ctgtcaccca ggctggggtg ca

#atggcatg  38400

atcttggctc actgcaacct ctgcctcccg ggttcaagtg attctcctgc ct

#cagcctcc  38460

tgagtagctg ggattacagg catgtgccac ctggctaatt tttatatttt ta

#gtagagac  38520

agggtttcac cctgttggtc aggctggtct cgaactgctg acctcatgat ct

#accagcct  38580

tggactccca aagtacctgg attacaggtg tgagccacca cacccagcct ac

#aattcttt  38640

attagagata tgtataacaa atattatgtt ccagtcaata gcttgacttt ct

#gttttctt  38700

aatggcacct gtgcataatc agacgagttt aattttgatg aaatctaaat ta

#gcaacttt  38760

taatctcatg tcagtacatt ttgtgtccta attaaatatt gtgaaaataa ta

#tcctgggt  38820

tttattctag aaatgttata gttttaactt atacatttat atttttgatt ca

#tcttgaat  38880

tttgggtgtg tagtatgaga agtgattcaa agtttttttt tgtacattta tc

#aaaacatt  38940

taataactat tttaaagata cttttattta taataaataa acataggtca ta

#ataaattg  39000

atataggtca atcacttgac cctatgtgct tgaatttatt tctggaatat at

#tttattcc  39060

attgatttat ttatctgtta ttgtaataaa accacactgt gttgataact gt

#agctttat  39120

tataagtctt gaaatcagtt tgtgtaagtt ctacaaatgt gttctttttt ca

#aaattctt  39180

ctggctattt ttggtctttg catgtccata taaactttta aattataatg at

#cttattaa  39240

tttttacaaa aatgcccatg gcattttcat tgggattgca tcagatctat at

#acatgcat  39300

gtatacatct atacatgggg agaattgaca tctcagcaat gagttactaa tg

#aatatgtt  39360

attatttctc catttttaaa attatttatt tcagtaatgt tttgtggttt ta

#agtaaatg  39420

gattttgcat atcttttgtt aaaattattt gcatgtattt aattttttaa tg

#ttattcta  39480

aatagaattg attttagtca ttagtttgat gcaaaataca gaaacacaat ca

#atttttat  39540

attgacattg tatcctatga cattgttaaa tttacttctt agttctggaa tg

#ttaaaaaa  39600

atttaaataa tattctatat taaaattatt gtgtcttcta aaattaaaag ta

#tttttctc  39660

tctttctttc tttcttaact ccatgacttt attgtttgtt tgttttgcct ta

#tagcactg  39720

gcttgactct ccagtacagt gttaaacaga ggtgataaaa gccaatatcc tt

#gtgttgtt  39780

cccaatttca gggagaagga ttagtttttc atcattttat atgatattgg ct

#gtagactt  39840

tctgtagata ccccttatta aattgaagaa gattctttca tttatagttt gc

#tatgagta  39900

tttattgtga atggatgtta aattttgcca cttgattctg aatttaaggc aa

#taatttgc  39960

tttttctctt ttattctctt gctgtgataa gttacatggt ttatttttga at

#gttaaatt  40020

atatttacat tcctgggata aaacacactt gatcagatgt gttattctac ta

#tatattgc  40080

tggatttgat ttagtaacat tttgcttaag attgttgtat ttttgttcag ga

#taggtatt  40140

ggtctataat tttcttttat tataacattt tttctcagat atttacatca aa

#gttatact  40200

accctaatat cagtagttgg ggaatgttct ttcctccttt attttcacag tt

#attatttc  40260

atcctaaact atttgataga attcactagt gaaaccatct gaacctggag tt

#ttctattg  40320

tggcagattt tgtattactt tgacttcttt aataaatata gaactactca ta

#ctttcttt  40380

ttaaaaaatt ttactttagg gccgggcgcg gtggctcacg cctgtaatcc ca

#gctctttg  40440

ggaggccaag gcaggcggat cacgaggtca gtagatcgag accttcctgg ct

#aacacagt  40500

gaaaccccgt ctctactaaa aatagaaaaa attagccggg catggtggcg gg

#cgcctgta  40560

gtcccagcta ctcgggaggc tgaggcagga gaatggcgtg aacccgggag gt

#ggagcttg  40620

cagtgagccg agatcatgcc actgcactcc agcctgggcc acagagtgag ac

#tccgtctt  40680

aaaaaaaaaa aaaattattt taagttctgg gatacatgta cagaatgtgc ag

#gtttgtta  40740

cacaggtata catgtgccat ggtggtttgc tgtacctatt aacccatcag aa

#tttctata  40800

tcattttata tgttatgttt ccaagaaact tgaccatttt atttaaaatg tt

#gaaaatat  40860

tggctcaagg ttttttgtaa tctgcagtga tattcctgat tttattcatg at

#gtgaattg  40920

tgtttacttt ctttttatac atcttaataa gtgtttatga aatttataaa tt

#tttccaaa  40980

taaccaactt ttatattaat taatgttctc tattgttttt gtgctttcca tt

#acatcgat  41040

ttctgatcat tatgattttc ttgcttctaa atattttgcc ataaggttct at

#gtattagt  41100

tccatgtggt taatagtgtt ttgcaaaatg tctgtatcat tatcactttt cc

#gcctaatc  41160

gttctaacag ttattgagaa ggaaatgata aaatatttaa ctacgattgt gg

#gttttttt  41220

ctttcagttc tgtccaggtt tgcttcatgt agtttcagtt aaaaaaaaag tt

#tttaccaa  41280

aaacatgcgt attgtgattg ttatattttc ctaattaact gatcctttga tc

#attataaa  41340

ctatcccttt tatctttggg gacacttctt gtattaaggc ttttttgtct gt

#tattaata  41400

taacacatgt tcttttattc gtggtttgca tcatatatct ttttctattc tt

#acttgtga  41460

attatttgta ttgtatttaa actgtggctg gtggacaaca aagtacgtct ct

#tgtgaaca  41520

acctataatc aggtctttaa tcttgtctga caacctctga cttttaaatg ga

#gtatttac  41580

ttcatttaga tttaaactta gtattaattt acttgacttt ggatgtacta tt

#ttttcttt  41640

gttttctata tgtcctatct catttttagt tcctctgttc ttgctttctt tc

#ttgccttc  41700

tactgggtta actatatatt tttagcatta tattttaatt cttctatttg ac

#ttttagct  41760

atgtttcttt gtattattat ttttcgtggc ttctcaaggg actgcaatat ga

#atacttga  41820

cttatatcta cttaatttat gtaactggaa ataaaataca tgaattttgc ag

#aagtattc  41880

ccatgtactt ccctgtagtt tctgctgtta ttgttatatt ttttgtactt ac

#ttttatag  41940

atgtcaggtt gttatacgtg tcagctatat atattaatat acgtgtgtat gt

#gtgcatgc  42000

acactcggtt tttcagctgt ctttctactg agctccttgg attctccgtc at

#gtacatat  42060

aattaaaata ttaggcaagg atttaagggg agtttagtcc caaactttgg at

#ctaactcc  42120

tctgtttcca actactttag cagcctcata ctttattctc tgacacctca ag

#ccaatagc  42180

tgcgtttttt ttccttttcc aagttcatac atgttatctg cagaatagtt tg

#atataagt  42240

tatcaccaga tcagagttcc tcaagttgga atattttgtt ttaaattctt ag

#ctgctttt  42300

gcaaaatttt tgcccaaaat atgaatctga gctcctttca aggtttttat ta

#aaatatct  42360

agtcacactg aacactttag tgtataatag ccttaatgtc acttggtgat gg

#gtagatag  42420

aagaggagtt tacattgcaa taatatatat ggctttaaat tgattattag ac

#atttttgt  42480

tctaaaaatc ttttgatcca agtgtggtgg ctcacacctg taatcacaga gc

#tttggaag  42540

gctgaggtgg gaggatcttt tgagcctagg agtttgagaa cagccttggc aa

#tgtagcga  42600

gatcccgtta gtacaaaaaa aaaaattagc ctagagtggt ggtgtgtgcc tg

#tagttcca  42660

cctactcagg aggctgaggt ggaggattgc ttaagcccag atgtttaagg tt

#acactgag  42720

ctatgaaggt accactgcac tccagcctgg gcaacaaaat ggcaccctca tc

#cctgtaat  42780

cccagcactt tgggaggcca gggtgggcag atctccaggt caggatttcg ag

#accagcct  42840

ggccaacatg gtgaaaccct gtctctacta aaaatacaaa attagtcagg tg

#tggtggca  42900

catgcctgta atcccagctg ctcaggaggg tgaggtggga caattggttg aa

#atcaggag  42960

gcagaggttg cagtgagcca agatcatgcc attgcactcc agcctgggca ac

#aagagcga  43020

aaccccatct caaaaaaaaa aaaaaaaaag aaaaaagaaa aagaaaaaga aa

#agaaaatc  43080

ttttggcaat cagagagttt tctgttggtt gctttatacc actgaggccc at

#gtccccaa  43140

cctcacacca tcctttgagc ctttgttctc attgtttctc cttccatctc tt

#ccttctct  43200

ctcgctttct catatgggct cctgaacaat cccaattctt tctttattct tt

#cttatgga  43260

tattttccca aagcttacag tcagaactcc ttgtgtatat tttaatcaat aa

#agtttnnn  43320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  43380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  43440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  43500

nnnnnnnnnn nnnnnnnnnn nntatttttg aaatctgtcc atccatccat tc

#atctatcc  43560

gtctgttgat ttgttcactg attcacattt ttgctaagca agtacttatt aa

#atatcaat  43620

gtgtgccagg cattatatgc ttatctgttt cttctgacct tttttttttt cc

#tattctac  43680

ttcactgtac attaactatt gatatagact caattcttca tctctggtgt tt

#tgataata  43740

tgagtactcc tttcaaatgt tcatttactc tcatgatttc aattacttat ag

#atacagga  43800

attctcccaa attcctttac gtgacccatt atgctaacac aaacctctcc tg

#tgataact  43860

ctgtttatcc tgctaacaag atatgaagct aacattttat ttcctcatac tc

#attcttgg  43920

gtaaatctcc acaagccagt atgtctttct aatttcttcg tcactctcaa tg

#gttcaatt  43980

tagtctccag gtgttatagg agtggtctca atagataatt aagttggctt tc

#taattagt  44040

cttctaagaa ctgtttcaga taaactatgt aaaatcaaag ttgtcatttt tg

#ataaatat  44100

tctcatgaga tgacttactg catgcagctg tcaaaactaa tgaatttata aa

#tcaagcca  44160

gctcattttg cctttgtatt agtctgttct cacactgcta tgaagaaata gc

#taagactg  44220

ggcaatttat aatggaaaga agtttaagtg actcacagtt cagcatgact gg

#ggaggcct  44280

caggaaactt acagtcatgg caaaaaggga accaaacacc tccttcttca ca

#tggcagca  44340

gcaaggaaaa gtatgagtgc ccattgaagg ggcgagcccc ttataaaact at

#cagatctt  44400

gtgagaccta actcactatc atgagaacag gatgggggaa accacaccca tg

#attcaatt  44460

atctcttcct ggtccctccc atgacatatg gggattatgg gaactacaat tc

#aagatgag  44520

atttgggtgg ggacacagcc aaaccatatt ggcctccaaa cagaatatgt at

#aactatat  44580

gtataactag ctttccttgt aacgatccct aaaaaatgac tttttctttg ga

#agttttat  44640

tgagaaattt tttaaaggta agtgtttcac agtatgtaat tttaagacta aa

#attatagc  44700

catggtttat atttggggcc ccaattgtcc aactatacat gcatgggaac ag

#atacaaaa  44760

caattttaaa atatcttact cctatttcat gcacattgaa tacatttata ag

#actaattt  44820

ggcacattag tatgaatggg tcagtccata ttctcagctt tgtagtagca ct

#caaaatcc  44880

tgtttgtgtt tcataacttg ataaatactc caaaatttct ctggattaag cc

#aggctacc  44940

agagagagtt gcacatgaac ggtatactta caatctctga agacatagtt tt

#aataaaac  45000

atatttgaga attattccac ctacgtactc agagtgacgt tgtaagaatg gg

#aaattttt  45060

ttttttggat ttttttaatt tttatttttt attatacttt aagttttagg gt

#acatgtgc  45120

acaacgtgca ggtttgttac atatgtatac atgtgccatg ttggtgtgct gc

#acccatta  45180

acttgtcatt tagcattagg tatatctcct aatgctatcc ctcccccctc cc

#cccacccc  45240

acaacaggcc ccggtgtgtg acgttccgct tcctgtgtcc atgtgttctc at

#tgttcagt  45300

tcccacctat gagtgagaac atggggtgtt tggtttttaa aataaatttt tt

#ttcccatc  45360

ccaaaaaatc agaatgggaa aaaattttta tttttattta tttatttatt tt

#ttatgaga  45420

tggagtctca ctctgtcgcc cgggctggag tgcagtggcg cgatctgggc tc

#actgcaag  45480

ctccgcctcc cgggttcatg ccattctcct gcctcagcct cctgagtagc tg

#gggctgca  45540

ggcgcctgcc actacgcccg gctaattttt tgttgtattt ttagtagaga cg

#aggttcca  45600

ccgtgttagc caggatggtc tggatctcgt gacctcgtga tccgcccgcc tt

#ggcctccc  45660

aaagtgctgg gattacaggc ctgagccacc gcacccggcc aagaatggga aa

#aattaaac  45720

atacttagta tttactgtgt atggatgttg tcgaggacat tgcttattaa at

#tatggtac  45780

cctctttatg ggttttttca gaagaaatgt atctttggtt tcagtatcag ag

#attattgt  45840

atttttgttc atttaattta tctttatttg cgataacaaa gatttactat ct

#tgcttttc  45900

cctttatttt atttatttgt tataaagagc caaatttata atttacttct ag

#gaagtcta  45960

cagtgtgtat tttgtcagtt aatctaattc ctgggttcat tccatttgtt tt

#acccctat  46020

tttctctgtg ctcaatatac catcacatcc tgtcaatatt tctttacatt tt

#tctttagc  46080

tctactccta attattttta acctaccaga tcattgtaat ctatgttgtg ct

#ttatatgt  46140

aaaatatcga acaagttttt atcaaacttg cctattccta ataacaagtg at

#atgaaggc  46200

tcagactttt aagtttatat gtcactaatt aatctctgtc tgttgtttct ct

#gtcaccta  46260

ctgtagtcac ctaaactgct ttcaaacttt cactgccttt caataacacc aa

#acctgata  46320

atcccccaca cttttttctt atgactgcct tacactattt tccttcagcc ta

#agattttt  46380

cccagccctt gacaaacatt ttgttgccac ttatgtatct ctccccgtac tc

#aacccact  46440

gaatcctttt tctttcacat ctttactaat cctcagttgt tccttcaaat ta

#gagctccc  46500

agatcttctt ctctgtcctg ttgcatttac ctctttgtag ttatttctat ta

#actaaaat  46560

atttttcatc tatgtatgaa aaggttctta tacattgact tgtatcctgc cc

#acatcatt  46620

caatgtgaat gttcaataaa tgctattgat taaattcaag ctagtgcaaa at

#ttgtagct  46680

ggtatgctga aatatgcttt gaacttaact ttgaaggtat ttccattttt gt

#attgatcc  46740

atactatatt aatgttagca aattttactt tgtgtatgtt taaattatct at

#ctgtatgt  46800

tgtgtttcta aaaggatagt agattattta aaatttctag agacaaaaat at

#tcttaaac  46860

ttgggaagat tgggttgata attttgtatg ttttataaat ataattcaca aa

#tataactt  46920

ttcagacatc tgttttgctt aaaagagagt acatctgttc ctgaaaaata aa

#aatatata  46980

gtcataaata aacaatttaa acttggtcat ccaacttagt gtacttatcc ag

#gtcaatga  47040

gaagtcaata caaaactacc ttcacaatcc tatcaggaga gtttggcaat tt

#ctaataac  47100

tgatattcag aagtttatag aataattaca ttttatacat gtattcatct tc

#taagtaga  47160

atttactggt cagtaaaaac tctcatgcat ttaaacctat aacaaattct tg

#cttattta  47220

gatctacaga ccctaatttc aaccatgatg aaagtatttt agtgataaga at

#aatttatg  47280

aataaaaatg taatttagtg aataccttgg cagttaatgt tgatcgcttc at

#cacagttc  47340

agtcatgttt agaaaatcta agcaagctgt gtgattactc caggaagcat gg

#aatgatgt  47400

gttcaaatta gtaccatctg tggatagaaa aagtttgagg tttttagtca tt

#cttaaaga  47460

atggaaatct gattctccat gctgaaatga gtgtaatcct ttttctatct gt

#aattcaat  47520

agggcagttg ccttgaaata gtctagttca gcacacagtt catcaaagag aa

#gatactgg  47580

atataaatga gggttactgc tggtcactta tgaatacttc tgaggtagcc tt

#gtttaaaa  47640

aattgtctac aagttatacc atatatttca cctcagatca gattcatttt tg

#gtttatct  47700

ttctaaatac atttgagtga aaatgtggac tagattttgt accacatgaa aa

#caaaaggc  47760

tgtttcaatg aaccatcatt tatttccaca gtcaacaaac acttatgagt gc

#cagtatgt  47820

tccagtgtcc catcactgtg cctgtcacat aataggaggc tgaaattgtc at

#tatgtttc  47880

ctatagccag gttacaaata actcttgcct ggatttagtg gtttttcttt tt

#aagacctt  47940

ttcttctctg aaagcttaat tggagaatac tagagtctgt gaacgaatat tg

#atctgctg  48000

aaaattttta ctgtgtagca aaatttgcta gtaacaaaca ccagctatcc ta

#aaatctga  48060

acattggagg aaaaaatagt tgatcataga ggcatgggca tctagtcatc cc

#tccagatg  48120

ggattagcaa agggcagcct cttctgcctt ctctagttca ttagctagtg aa

#tatttccc  48180

tctcatttcc agtggtttag caaactctag ggagagaaaa ttgaaacatg gg

#aaaggtaa  48240

ctggtagtag atctaaaaaa gaataaataa aagaaggaaa gcatttgtac ac

#tgattctt  48300

atgaggaaag agtagagtgt aagattttaa tgaaaacaaa agtcaatata aa

#aattttca  48360

aggccaggtg ccatggcgca tgcttgtgat cctagcactt tgggaggctg ag

#gtgggcag  48420

atcagttgag cccaggagtt caagaccagc ctgggtaaca tggcgcaact cc

#atctctac  48480

aaaaagtacg aaattagttg ggtgtgatgg cacacgcctg tgattccagc ta

#cccgagag  48540

actgggttgg gagggtcata tgagcctggg aggttgaggc tgcaatgagc ca

#tgattgtg  48600

ccactgcatt ccagcctcag caacagagta agactctgtc tcaagaaaaa aa

#aagtaaaa  48660

atttccacat aataaaaacg atcatcagca aaataaaaag acaaataaca ga

#tttggaaa  48720

atatatcttc atcaaggatc acaaaaaggt tatattaaag gcttaaataa at

#aaatagca  48780

aagccataca cccaatagaa aaaaaagtag aaaaaataca aatgatagtt ta

#tgaaaaag  48840

gaaattaaag agagagaagt gcaataagct tcacactgaa acagaatttt tt

#ccctctga  48900

aacttttatt gcatactctg ttggcttggg gaacaggcat tctcatgcat gt

#tgacagga  48960

gtaggtattg gtaaaacctt tctggagtgt gatttagcaa tgtctaccaa tg

#ctacaaat  49020

atgcatatgt tttacctagc aacgattatc ctacagattt actcacacat at

#gtgtggaa  49080

tatgaaagca taggcttgta cattacagct tgtttactgc aaatattgga ca

#cagcttaa  49140

tatcatttca tagaggctgg taaaatcaaa ttcgatatat ccatttcaat gt

#gcttactc  49200

catacaactc caaacactga agaaaaaggc aaaatatatc caaagcatat at

#atagtgac  49260

cctaaatccc agtttccagg gcagtctggg tttatgcttg ctgtgctggc at

#ttcatcca  49320

atagacattg ccttttactc tcaaaagtga cctgtctgta taacaaataa tt

#tgctgcaa  49380

tggagtgaat gtttatttcc cccctagaat catatgttgt agtcctaact cc

#ctgtgtga  49440

tgctattaga aggtgaggcc tttcagaggt aattaggtca ttagggtaga ac

#cctccatg  49500

aatgggatta atgcccttat aaaaagaatg cagagagctc ttttgccctc tt

#tctgctat  49560

acaaggacac aataagaagc cagcagtctg gaaccaggag gctggttctc ac

#cagaaacc  49620

tgccatgctg gcaccctcat ctgactttca gttccagaac gttgagaaat aa

#atttctgt  49680

tgtttgtaaa tcactcagtc tatagtaact tgctacagca gccaaactaa ga

#cagtcact  49740

ctagatatat tctttggaag tgagagaagg gactgggagg aagaagagag tc

#ggatatga  49800

ggttagatga aaagaaaggg aaggctgcac tccagcctgt gcaacagagt ga

#gaccttgt  49860

caaaacaaaa caaaaacaaa aacaaaaaca aaacaaaaaa aacttttcaa gt

#atatcact  49920

gtgcttcaga taaagctaaa agtcatatat ttggtttaag gactcagttt aa

#tgtgattc  49980

cacttacatt gcctacttcg ccccttccca ttcatctcct tgtagaccca gc

#agccttca  50040

ttaagtgttt tcaatgtgtg acactcttct tcttcagggt cttaacacct tc

#acacatcc  50100

tgatattttt tatctgtgaa gcttcttctt atccttcagg tctctaatta aa

#ttatcctt  50160

caccaggaaa gccaaataaa gtcccctgtt ataccctcga ttcatttgct ca

#cacattca  50220

aaaaacattt attgaatcac cattttgttt cattcagtag tctagactct ga

#gagagaaa  50280

atctgttatg tacattgaca ttttatgtgt ttataaccta caaataaaca tc

#tatttttg  50340

agtgattttt ttcaatattt ttattgagat ggatgcggaa gcaaaatctt tt

#tgaatctt  50400

ttatatataa tgttggggtg atgtggaggt gaaagtagat taggcctctt tg

#gagtgggt  50460

tttcagtgca gagctgagat ttataggaaa gtaaatatca gttcaaaata at

#gcaggcta  50520

tacaaactaa tagagctatc tgaaaatgaa ttttaaaata aactaccctt ga

#gatgttga  50580

gtttcccttg ctctatgtat gtaagcagag gctgggtaag tagtgattgc tc

#attttgta  50640

gggggaggtt tgtattggtc ttctattgct gtgtaacaaa ttatcacaaa ct

#tagtggct  50700

taaaacaata cccatttatt agctcatagt tctataggtc agaagtccag gc

#gtgttgtg  50760

cctaggtttt ctacttattc tcacaagctg aagtcaactt gtcagtcaac at

#tttcttgt  50820

gtaactcagg gtcctctttc aggttcacgt ggttgtggta gaatttagtt cc

#ttgcagtc  50880

acaggattga gagccccttc cctggctggc ttttagctaa gggcttctct ca

#gctcccaa  50940

agatgttctg tgttccttat catgcagctc cctccttctt gagagccaac aa

#tagagaaa  51000

tttttgtacg ttgaatccat ctctttaaag ctttgacttc cttttctgaa aa

#cggccaaa  51060

aatactatcc tccttttaga gggctcaggt tataaggtca ggtccactgg at

#agctctct  51120

attttaaagt aaactgatta agattcttag ttacagcagc aacatagctt tc

#catctgta  51180

cctagatcag tgtttggttg agtaagtgga agaatctgtt tttgtacagg ga

#ccgggaat  51240

cttgagggat atctgcagac acaaaggtca gacaaagaga caagatgacc aa

#gatgatct  51300

ctcaatttca aattctgaga ttacgtgatt tttctcattt atttgcctgt tc

#ttatggat  51360

tcagtcgcca aaatatatct taaaaactga cttctgtact gttgctatca cc

#taatttcg  51420

tctttcctgg acaaatcaag tagtctctga atttctccct tttcctgttt tg

#caattacc  51480

agactgtagt tgataaaatg tacctctgga catactgtga cattttttat ag

#ctttcaat  51540

tgcctgacaa gctatctata gtttcctctg acacagtaag tccccaagct at

#tgtgcagt  51600

cttgctgttt gttattgccg acatgaatta caagctgcaa ttaaacttgt ct

#tagctcac  51660

atcaccctct cttccatgat ctccactttt acaatcagtg aaattccatc tc

#atgtgcca  51720

tctcttctct aaaaacattt tctgaacacc cacgtcaatc aaatacatct ga

#tttatatt  51780

agaatatttt gaaaatgtat cttatgttca gatgatctga gttcaaattt ag

#tgactgag  51840

gcatttgaaa aaattatgaa aattctaaaa cttcttcctc tataaattta ca

#tttttttt  51900

ccctaaagat agtgttttct ctaattgctt ttcttcatga taggtaaaga ta

#aaacagaa  51960

tgtgttgtaa atagtgtgcc agttttggta aatatatata tatatatagt aa

#ataagcaa  52020

tagatctgta aataattcga taaaaattta agatgaaatc caaaatttta ac

#tgaagtcc  52080

agacctctct ctacagaatc cagactcaag cttctatcta gtatttgatt tc

#tccttctg  52140

ggtgtctgag aggaatttca aagttaacct actcaaaaga aattgttaat ct

#tcctcccc  52200

aaagcttacc cctcttacgg tcacccacat cttgattaat agtgacttca tc

#tttttatt  52260

tgctcaatcc ataaacctta gggcattttt tattcctctc tttctctgat at

#ttcacata  52320

ccacacatca gcaaaccctg ccagctctcc ttcacattat attcaggagc tg

#aatgtttc  52380

tcttcacttc tgccactacc accttggacc aggccactgt gatctcttgt gt

#tgacattg  52440

cagttgcctg ctaattactc tccagccttg ttacccttta gtctgttctc aa

#cacagtag  52500

ctagagtgat tctgtgaaag agagagcctg ccacttctct gctcaaatga aa

#gccatgac  52560

aatgtcctct agtgtcatgt actggtagct tgtaccagtc actcagtcct tc

#ttgttatt  52620

ctccaaatat accaggcatg cctccaacta tacagtttcc tctgcttcaa at

#ttctcttt  52680

ctgaaatatt gacatggcta ggtcccctac ctacatatgg aatttagtat ct

#tctttttc  52740

tttttttttt tattattata ctttaagttt tagggtacat gtgcacattg tg

#caggttag  52800

ttacatatgt atacatgtgc catgctggtg cgctgcaccc actaactcgt ca

#tctagcat  52860

taggtatatc tcccaatgct atccctcccc cctcccccca ccccacaaca gt

#ccccagag  52920

tgtgatgttc cccttcctgt gtccatgtga tctcattgtt caattcccac ct

#atgagtga  52980

gaatatgtgg tgtttggttt ttttgttctt gcgatagttt actgagaatg at

#gatttcca  53040

atttcaccca tgtccctaca aaggacatga actcatcatt ttttatggat gc

#atagtatt  53100

ccatggtgta tatgtgccac attttcttaa tccagtctat cattgttgga ca

#tttgggtt  53160

ggttccaagt ctttgctatc gtgaataatg ccgcaataaa cacacaagaa aa

#aaacaaac  53220

aacccccatc aaaaagtggg cgaaggacat gaacagacac ttctcaaaag aa

#gacattta  53280

tgcagccaaa agacacatga aaaaatgctc atcatcactg gccatcagag aa

#atgcaaat  53340

caaaaccaca atgagatacc atctcacacc agttagaatg gcaatcatta aa

#aagtcagg  53400

aaacaacagg tgctggagag gatgtggaga aataggaaca cttttacact gt

#tggtggga  53460

ctgtaaacta gttcaaccat tgtggaagtc agtgtggcga ttcctcaggg at

#ctagaact  53520

ggaaatacca tttgacccag ccatcccatt actgggtata tacccaaagg ac

#tataaatc  53580

atgctgctat aaaggaattt agtatcttct aatcccttct ctgattacct aa

#tttaaatt  53640

ttcaatatcc ctgaaactct ccttcttctc attcttcttt ttctccgcaa ct

#ctgatcat  53700

catccaaaac actacagttg gccctccaaa tccatgtgtt ctgcatccgt gg

#attcaatc  53760

aactacagct ggaaaatata caaaaccaaa atgtgtctgt accccacatg cc

#cagacttt  53820

tatttcttgg cattaatctc taaacagtac aacagctatt tatagagcat tt

#acattgtg  53880

ttaggtattg taaataacct agagattatt tgaattatat gagaagatgt gt

#gtagttta  53940

tatgcagata ctacaccatt ttatataagg aatttgaacg tcttctgatt tt

#gttctccg  54000

tggaaggtct gggagccagt accctgtgga tacaaaaggt gactatgtac aa

#tacttatt  54060

gatactttta ttgtttacag ctcccctgaa tgtaaatttt caggggcagg aa

#tttttgtc  54120

tgttttgttc attgtatttt cagcacctat aatcctacct gtacatatta ga

#tgctctta  54180

gatatttatt gaatgttgaa ttaatatatc tttagagatc aatgagcttt ct

#aaatattt  54240

attaattttc ttattttaaa atgtgaatat taatatacag ttcgcattat gt

#aattttca  54300

catgtcatca ttttgattct ctttatctcc atcttcttaa caaaggccgt tg

#aagatata  54360

caaagaaagg catggttaag aagagttcca atatcactta attgattgct ct

#ttcttatt  54420

tctaaccata acatgtgtat attacttgcc caataaactg tctcttgaaa ac

#aggacatg  54480

agctttattg tatcctgaat ccctaacacc aggctcagag cctgacacat gg

#tatgcatt  54540

tggcaaacct gtagttagtg tggaagcaaa taaatgactc caagcaggac ta

#catgttaa  54600

ttctaaatat acatgagata aaataaaaaa aaatagatga attatataca tt

#aaaactgt  54660

cagtaatatt gtttatttaa aattgtttta taatcaacat ttannnnnnn nn

#nnnnnnnn  54720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntaatgcc cttgacccag gc

#ccacattt  54780

ttccttcata tttagaggtt ctgttgcttt taagcccaac tttacaacct tt

#tcagtgac  54840

ttcaaactta cacacacaca cgtgtgacca caataaccct gattgatctg tc

#tgcaagtc  54900

gtttttcagc ttgtgttttt caactgcaca aaattctgag gcaaagaaat at

#caagcatt  54960

caactcccag cttgagatgg gaagaagaaa atacagagaa gaaacacaaa ta

#cttgaaat  55020

tgttttgcca tctatacatc tttcaggact ttaagtgctt ttccatacaa ac

#cactaaat  55080

gtataggtaa agattgctct tgcaacttag gttttatgtt tatagctaac tg

#gttgccct  55140

gcttgcttgg agaatatcat taaccataat taagtaaaaa atgtatattc ct

#tatcctga  55200

actctgttta catagaattg tgatggttac tatgcaacat aaataagttg ca

#aatcaagt  55260

cctgcaagcc agagctctgg gaaatggctg cattctctga aatgccattt ct

#gccccagc  55320

cctccagagc aaatttcagg tttgccaggc cacccatccc atataaatcc tt

#cagatata  55380

ggccttatgt tatcatcttc ctatcttgac tgagactctt taaaggggat tc

#ctttcaaa  55440

tccaaattac atattcttaa acatttttga tacttattag tatagtaaca ta

#cctacaca  55500

cacacacata gattttcagt gacaaatacc atgttagtac ttatagatag tg

#aaatacac  55560

tttgatctag agggctttat tttctaggcc accaattgtg tctcctgtta ca

#atttccca  55620

gagtatctgg cataatgtct gtaatagtaa atgttcaata aatgtttgtt aa

#tataattt  55680

gacatttgag gtagaatcct gacaactcag actttgacac aattgtccaa cc

#ttttctct  55740

ttctggcact ttgacacttg gtttctgtaa gatctaccct tctgtttttt ct

#cctacctt  55800

cctggtagct ccgtctccgt cctctttgct gaattattct cagcaaataa tt

#gtttttta  55860

actgtaagca ttggagagat gagagagctg tacttggccc ttttctcttc tt

#tatcagca  55920

cacaaccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  55980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  56940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  57960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  58020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  58080

nnnnnnnnnn nnnnnnnnnn tgccgtggcc tcccacagtg ctgagatttc gg

#gcatgagc  58140

taccatccca cgcatatttt cttattgtct agaatagttc ccagtaccag ta

#gatactct  58200

ataaatattc cctgaatgac agaaatttga ttcattaagt tctagtgggt ag

#gacaaaac  58260

agattgtttt ccagtgcagg tgatgataag gatgacgata ctataacaaa ga

#tagcaaaa  58320

atatagaaaa aattcagttt actttggggt tagtcagtag gtatggttat tt

#tgaacctg  58380

ctgattttgt caagaaatag ctattctaat ataactagaa aacagaaata aa

#taaatgcc  58440

cagagaacaa aaccaaatac atcagtagcc tagatctggc ttgtggacag ct

#gatttgta  58500

acctttggtt aaattatgcc tgtgcctttg tgcctgctgc cactaagagt tc

#ctattctg  58560

agtatctcac ttggggagta cctgcttcta attgccacac tgagccagga ag

#tgaacttt  58620

ttaaatgcac agtctcttgc aagcacactt gacactatta tacaagatgt tt

#aattagca  58680

taaaacaaca tataacatga tcagttcaga agttggtaaa tgaaactgaa aa

#gttggatt  58740

tctaaataat cctacattct caagtctttc cacttgaata tcattctttc ca

#ccctattt  58800

cctccacttc ttaccccctt ttaagttcta tggccatatt ttatttccag ga

#gacacagg  58860

ggaaatggtc tttctaccac tgtgattagg agagaaagat gaaaagattt at

#atttttca  58920

acttcgtgat aacaaacata tgattgcatt ctcaaaactc atagcttttc aa

#ctaagtag  58980

tcataagtgg ttgaggataa ttctttaaat tttgacgatg agttggttac tc

#gtctttta  59040

gtttcaagaa tggaggaaat ttttgcttcc aatggaatag aagacatttt tc

#taatgata  59100

aatattgtac aattgaattt ccaaatttca taatttatac atcaaaataa aa

#gttctatt  59160

tattatatta agtcaggaag agataatttg agattatatg gggaactgca ta

#tattattg  59220

caacataata tatatggtga aataacataa gaataaaaga aattataaca gt

#taagtaac  59280

ggaagtcttg aagagcaata atccttttaa tattaaaaat aaggcattca ta

#gatgttgc  59340

ttctgcatac caaagatgaa aatataatgg ccatgttgca aactcaaaaa at

#aatttgga  59400

tgaagaatat taataagttt tgtattatgt ataattcact taaaaatgtg gc

#atgagtca  59460

tgtggtggct catgcctgta atcccagcac tttgggaggc tgatgcgggc gg

#atcatttg  59520

aggtcaggaa tttgaaatca gcctttccaa catggtgaaa ccctgtctct tc

#taaaaata  59580

caaaaaatta tccgaacatg gtggtgggca cctgtaatcc caactccttg gg

#aggctgag  59640

ggaggagaat tgcttggacc cgggaggtgg agcttgcaat gagccaagat tg

#tgccactg  59700

cactctagcc tgggttacaa agccagactc catctcaaaa ataaaataaa aa

#tgcaatat  59760

gttgtttcat gatataaaat aaaataataa ctctttctct gaattagaga aa

#agactaaa  59820

caacaatata aaatagtaca aaataactat ctcagagaac tgcattttat cc

#taatgaca  59880

taaagttgta ctcaagcact tactaatata acatcttgtc aaaacctgga tc

#ttctctat  59940

aaagagttat tgattaatgg gtagtttgaa atcaaattgt ttaaaatttg ag

#taactcca  60000

ataaaagacc acctagtttt aataataaat attataaaag tttctacaat gg

#attatata  60060

atcagaaaac atgttatcat taactatctg agcccataac aaagagcatc aa

#aattgaag  60120

atcaggaaga aaagtcagaa tgcaagctga gatttaaatt ggattaccct gt

#gaatctga  60180

gtgtacacct gtaaaacaga ataaataagg gaaacaatat tcaaccaact ca

#agctaacc  60240

attctttctc tcacatgctc tcacctagat cattgaagcc aaattgcttt tg

#ttctcaac  60300

taatccgtat aatagccata atcctactgc atgctgagag tgtatagata ca

#aatataag  60360

cataaaaatt ttaaaaaatg gcagaaatat ttaccttgaa acattacagt ca

#tgcaaatt  60420

attttactca tctatttttc tgattatcct taaagtcaaa agcagtttga gt

#ggtgtgtg  60480

tatatatgtg gtggtgatgt aaagtcacaa gctgttaaat gtttctgtgg tg

#cacaatag  60540

atacttatgc tgaggaaatg tacaacttta aaggagtgtg ggtgtgaaat ta

#gtatgaaa  60600

tggaatggga ctctcataat gtgcgtctcc tatagaccac caagactgga ag

#acagcaag  60660

aaaggaaaat tcctggggta acacttaggt tgggaaaacc acaggatacc at

#actcatga  60720

ggaattttaa ctacccaaac atctgttagg ttaaaaaaaa ttcaacaaaa ca

#tgcctcat  60780

caaagaagtt tctaaggaat gcaactttat gatctaaaaa gaagaaaacc aa

#atagaggg  60840

caaagtacac tttaacatta tttaaaatta aaaattgtca atgtgttact aa

#atatcagt  60900

tgttttcctt agttttttct aaactgtgta atacacttat gtgataagtg tt

#atagtaac  60960

agaggtagaa attatccttt ttataaagaa gcaattatat aatggtaaga ag

#tgatttta  61020

gccataagta aataggagtc tataattcaa gacatttaga agttcatttg gt

#ggcagtgc  61080

agtattagga tgggctccat cttgctgcca ctagagaaaa taaatatcat tt

#attctaga  61140

catgatggtt gcacttctgc aaaattagtt agatgctgtt gaaaatcttc ta

#aattagtt  61200

acacaggact ccctaatggg taattcaaga caacatttct gtcctctagg cc

#cgaatatt  61260

gaagttattg gtataaccac ttaggttccc atagacatct caaactccat at

#tgccacct  61320

tcccttgcaa gtcttttcct ttctgtgtgt tccgtgtctc agtttactgc ac

#cactattc  61380

atctagttgt tcaaactagt tatctagaaa tcattgttag ttctttttac ct

#actctcat  61440

cccccacgag gcaaaacctg agtcctattg tatttacctt ctaaatatct ct

#tgtatttg  61500

tttatttttc ttttcaagtg tctctaaatc cagactttta catttatctc tt

#agataatt  61560

acaaaagaga tctaaatggt ctttctgctc tcattttcta ccattcacct ga

#actcagca  61620

tttcaataca cctgcctgac catgaatttc ctctgctgaa aatctttgat ca

#ttttctac  61680

atgcctgcat gttaaaacta tgcccattag taagttctac aaggtcactt at

#gatttggt  61740

ttatatttct cacacatgca ctgttctttc tcctttatga tccagaatct tt

#gctattct  61800

ttcttattgt cctttctttc ctcctcttct ggtgaccagc tttgttttcg ct

#atgttttt  61860

aagtatctct tttgagaaac ctttcagaaa tccttatttc cagtcccacc tc

#ccaaaatt  61920

tgtttctcaa caagtgagca tattacatca tagttatttc tctctctttc tt

#tagtgggg  61980

ctttgagttc cttaaaagca taaatagcca gccgcgcatg tcttatgtac ct

#ttctgtcc  62040

cctgtgccta cttttaatct aagatttgtt atgaatatgg aagaaaggca tt

#tgacttta  62100

atgttaaagt gttacagtgt caaaattctc catattttaa aatagttcat gc

#tgatattt  62160

tttaattttt ttggtctaat gcttgtcttt caaatgcttg cattgttatt gc

#caaaatta  62220

aaattctctt ggccagtagc ttttcatgtt tgatatattc agcttctttt at

#ttcacaaa  62280

accagtatat atttattatt attattatac tttaagtttt agggtacatg tg

#cacaatgt  62340

gcaggttagt tacatatgta tacatgtgcc atgctggtgt gctgcaccca tt

#aactcatc  62400

attagcatta ggtatatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  62460

nnnnnnnccc ccatcccaca acagtcccca gagtgtgatg ttccccttcc tg

#tgtccatg  62520

tgttcgcatt gttcaattcc tacctatgag agagaatatg cggtgtttga aa

#accagtat  62580

attttatatt gttgatcatt ttgtgatttt ccttttcatt aattaacata ag

#aaatataa  62640

attattgcat ataggaaata tttgtattgc atgtaagaaa tatgaattat tg

#catataga  62700

aaggaaataa ttattgcatt tagaaatatt tcaaacagtg aaggaaaata at

#aaatgtcc  62760

atttcagaat agattggaga agcattaaaa atatctaaat gattaactga ga

#taattagc  62820

tggtaataag tatgtatagt gagacagagt tataatagat tgatggtcca tg

#aaagaaca  62880

aaggggaaga acaatgttta aatttagagt gctaagtgtg attacaaaga gg

#agatatat  62940

gggtgaaatc ataatttaaa ggaaatgata gaaagcatat aacattaaag ta

#tctaataa  63000

agtattcaac tatatattta atgtcaaaag accttatgct agattatgat gc

#aaatattc  63060

tagaatttaa ataaaaatac ttgtttttga aatcctattt acataagcaa gt

#agaaagtt  63120

gtagcaaaat cactaaaaat caaacaaaga aaagtgtaaa gattatcact gt

#tttttttt  63180

aaatcatcaa tattttagaa agtctgattt tcataaagga aaaaggggag ga

#aattttct  63240

ccccattaat agcttagctg tattttatct ttttaaactt caaatgaatt ct

#cctatttt  63300

ctctgagatc tcagactaaa tttcacattg aattgaatta acttttactc tt

#ctgagaat  63360

cttctttctg tccattcaac aagaagtgta aagtaggtgt aatacattgt ga

#atttttgt  63420

ctttaacctc agttctaagt tctagctcag cattaggccc taggtcagca aa

#atttcagc  63480

tcctatttct tctgcattta ccaagaaaga attctgattt aactatgaaa at

#tccaaact  63540

atagaaaaat cctgggatta ctatgtatgg tgtcttggtc actttttgtt ca

#tgcctagt  63600

aaatcaattg agatccatag gctgcacagt taagaatatt agcaatgact ta

#ccttactg  63660

tgtgtttgct atgtgctggt actattctaa gtacttaaaa cattgattca tt

#cataagtc  63720

tttatttcta gcacagagtc tacactctta gatcttgact aggacttgag ca

#aagcctca  63780

gggtggtaga aaagtaccaa gagatggaga ggtactaact gatatgacat ag

#agaagcta  63840

tccaactgtc caatcatctc ccaaaacaaa ttgagtgaga attttgacat gc

#acctcaaa  63900

attatacttt tgaggtttct gatacccttg attttcattt ttctttaatg at

#atcctaga  63960

tattttttac cccaattatg cctgcataca aatgaacagg agaagaaaat ag

#caagattt  64020

atcctggccc taagactcca gtatgacgat ggccttacct gaattattcc ag

#tttgttcc  64080

aatgcagagc ttcatggtag catgaaaatg gtgatatttt atgctctaat gg

#aacacgct  64140

gacctgttgt tctaaaaact ttgggaattg gaggaagtga gtagggagaa cc

#ctcttcat  64200

agtttatcca gaattaaaat agaatgaaag ataggagacc agtctgtgga at

#atttgatg  64260

gcttgataca tgtttccatg ttgattacca gctcccaaac ttcctttaca tc

#tacttccc  64320

tagtcttcac agaaggagta attcaatccc cttttccaat ccactcattt ct

#aagagttg  64380

taatcattgg tcatctaatc tgaagagcag tgtcactatt ttttcaaatg gc

#atgtcgac  64440

gttatagagc agtgattctt aaacttgaac tacagtcatt acaaccacct gg

#aggactgt  64500

taattactaa gccccacact cacggttttt gattcagtaa gtctggggta gt

#gcctcaga  64560

aactgctttt ctatggcttc cccagtgatg ttgatgctgc tggcctggag ac

#acattttg  64620

aggaccactg ttgtagaaag ttgttttata aacatgatgc tattctcaga aa

#atatgtat  64680

tctctgattc caaagtaata gtagtaatta gaatattttc attcttacct gg

#catgtcca  64740

gtattgaaac tgagaggttt tctttctatt ttgtattttt tttctagctt aa

#gccagtct  64800

gaaattagtc aggaaataac tcatttaagc atcaaataag atgatcatac ag

#tgaggtct  64860

aatactatga acatccatga atcattctta gtattcatga atctaatctg ac

#aaattctt  64920

aggcttactg tatttgtaac actattgtgc tataccctct gcagcaccac ct

#tgcggtta  64980

ggaaatctaa ttagaaaaca cacttaacat ctcataaaat gataggaaat at

#ttcctaca  65040

ctgacagtgg tgatgcgttt tggtcagcga aatcactggg ctctaggaaa ac

#atccaaac  65100

tacaaaagga tagccagtta tcaaagtgtt ttaaccagtg gacaggaata tg

#tcctgaga  65160

tactcttgct gtgtggaaat aagatgaatc caattgcaga gcttcttcag gg

#cccttgat  65220

gccctgaatt gcttaagaca caggaatcca ccagcgagtt ggatttcttc ta

#gtcctgag  65280

agacatctaa cagtcagtgc taatttgtcc aggtgtgctg agtcaaagtc ga

#cttgtagt  65340

ccttgaagtt gttaatattt gtatagctga gaaaggacag agcccttcac tt

#agtgatga  65400

cagtcactag aaatctggtg gcctagtgca ccaaattctg aaactaaaac ac

#cctgagtg  65460

gtaggccctt ttaataaact ttatactgaa cttaaattca aataattgtg ca

#gacaattt  65520

aaattgaagg tatatagagc tgaagttttc tgttttgtaa gttgatgtta aa

#ccatatat  65580

tcatttatgt ttattctttt aggaaagtga tcataacgag gtacactaaa aa

#ccatagag  65640

tattttctag aatattttcc actattaagt tagacttaca gggatctgca ga

#tggcaaag  65700

ttacaaataa gtctttgaat gtgcttattt taaaagtata gtatcaggca ca

#acaaaact  65760

tgttgattct taaacaaagt ggcatggatg ggggcattca aatttttata tg

#gactaggc  65820

aaaatgatgg tctatccaga ctcacctttg agctaacaca ctcagcatca ag

#acacagat  65880

ggatgggaaa gatgaccctg acccacataa gacccacatt cctgtgcaag ga

#tagaagca  65940

tgataatcag gaaatgatgg ttgttagtta cagaaaatgc attatggtga aa

#accagggg  66000

gaaagtgctt atgagaggaa gtgattggat tatggggaat gagagaataa gg

#atagatca  66060

ttcctactga gtaaattgct gtgatttata agagaaaggg tatagtgata tc

#ttggccca  66120

ttactgacta gattcatttc acaaacaatc ctaaatcaga atgtgacagt tt

#atggggag  66180

acaaacaaag ctcccaaaga tgcttataag ttactaagct tttaaactgg ca

#tattttca  66240

actcctcata cttttgccac catcagctcc atttattttt gtagctgaca tt

#cataaatg  66300

taattctgta gccaccctta tgtgacacta gtaatagttt actatatgct gt

#gataaaag  66360

ggaaaatgct ggatatattt tattatatgt tttgggattt tgttaaattt ca

#taagaaag  66420

atacctaaaa taattttata tgtatttaag tatattataa tactctacaa ta

#ctaaaata  66480

attaagtgct gttttataat agatgggcat tttggtgttc taaatatttc tc

#ttaaatta  66540

cctatgaatt aatcaaacag ttacctttca ttgctccaga caggtgaaac ac

#atattgtt  66600

atatattata tattaatata tattcagtta tataagttta cttttatttt tc

#agtttgga  66660

tttaggagct ctaatccttc aagaatacaa gttgacaaaa tacattctga ag

#gaaatttt  66720

tggcaaagga ttcaacacag aaaacttgtg taacaagaca ggcaatttta tc

#aagaactt  66780

tactgaaaat gcaaacattg tttacagtga ttgtttcttt taaaaaatga ag

#aaagaagg  66840

gaaaacgatt tttggaaaag ttcaagaaat ggcattaaag catagctcag ca

#aggggcta  66900

aataccttgc ttttttataa tgattgatca gtgcaaggaa attaaaatat tt

#agtagtat  66960

aggtgattat atgtgttgtc atgaatgatc tttgaatgtc atttttctct ta

#cctctgct  67020

tggggtcaca cactccctga tgagagattt gattgctagt attaaaggaa tg

#attgcagg  67080

gttgacattt tattgtgaaa gaagagaagt tgaaagcaaa gcgctatatt tc

#tttctgag  67140

ctggcataca gacacactca caagccagag ttttccttgg gaaaactttg ca

#ctttgtcc  67200

tcaaatgaga cccgaagaag ccattataga gcagagatac agaaattttt cc

#agatacaa  67260

gctaccgcag aaaaatctca caactttctt agccgcagaa aattctcaat ac

#atttttca  67320

tgatgtctgg gcaacgataa tgtgccactc tacttgcttg ctagaatgag tt

#aggttgaa  67380

aagtatagtc ccaacagcat cgagtagtat atgttacaga ggtacatgaa tc

#aaatagat  67440

gttggagatg atctttcctt tttgacgtaa ttaattttag cccatctttc tg

#gtatgagt  67500

tagataccaa ggatcacagt ctatcacagc cccttctact tcatggcgtt tg

#tctttttg  67560

ttcacaatag ccatcctaac aaaagaagac attaacgctg gtcttaacgg cc

#ttacattt  67620

tctggccccc atttcctctc ggattctctc attccaactc aactgggctt ca

#gcttcact  67680

gtggtccttg ttattcttag gacataccag gcgaaatcct gcctcagggt ct

#tcacgctg  67740

gccattctcc tcctggacac tctttctcca aacagccaaa cagtattccc tt

#acctcctt  67800

caaatatttg tttaaatgtt atctgcttag tgaggcatga gctgaccact at

#atttaaaa  67860

tagtaacgcc ctaagcatct tcatgcccat gaccttgtct catttttcac ca

#tggtacat  67920

aatacttcct aacatggtat ataatttact tgtgtattat atattcattt at

#ttatattt  67980

ttctaatata tattatttgt ctctctccat taaaatggaa gttccatggt ct

#ttgtctct  68040

ttggttctct tctatatttc cagcacttcc aaagtgcctg gcatgccata gg

#tgttcagt  68100

aaactgttgc taaatgaaaa gggttaaaca gtagaagctt tatggatgga tc

#caaagcta  68160

ccttgatcac ctgtatgagc tttttgtcct cttagtgcct agcacatagt aa

#gcacttaa  68220

taaatattta ttcattcaat gaatgcataa atttattctc aaggccaact aa

#acatttgg  68280

ttataataaa gacaagggga ctctaaaata ttttcctgtt ttataccact tg

#aaatgtgt  68340

ggccgatcag aaaattgttt ctgtccacac tggttcttac agagctggaa gt

#caaatttt  68400

tcaaataaca ttaataataa gggagcctta atacatttat acagaggtca ta

#tcccatcc  68460

ccttttatag agtcagaggc agaagagagg ccattgaaac ccacaaagca tc

#ttatattt  68520

atatttttca aggcaattaa ttatgctgat ggcaggagac ctcttatagc tc

#tcatctgt  68580

tatgtataat tacctaaatg aattaggcta caatttgagg cagttttcct ag

#gaccataa  68640

agctagcagt aaaaagaatg aaaatgtctg tttatgcagg gtatgtgtat ga

#ttccttga  68700

taccttagtt gttgcagaaa ctgtgtaccc aattctgtct tcatcattag ca

#tctcttag  68760

ctcatcaaat cgaatcctgg agcattcttt ctttaccctc tcccctggat gt

#tttcttgg  68820

caatgtaaaa ctggatcttt gagtacgggg tgtcaatgtt cagattattg ta

#cagttttc  68880

agaagtacaa ataggaagag tatctttgtc actccaaagg tatttgttca ct

#gaaagttc  68940

ctgaaatgta ttttctagat tcctgtatag ttatattcaa gtactattat ta

#aaatatgt  69000

caatgctatt attaaaatat ttttggattg agttgtgcac ctaaattcca ta

#gacataat  69060

gttatatgcc taagaaatat attctaaata tcaattactt attcacagtt ta

#aagattgt  69120

caccactatt aatctcttag tctgttttgt gttgctataa gaaagtatca ga

#gacttggt  69180

aatttgtaag aatagacatt tgttttctta tagttctgga agctgggagg tc

#caagatga  69240

aggtgcagac agatttgctt atctggtgag ggttgcaccc tctggagggg ag

#gaacgcgt  69300

gtcctcacac agtggaaagc agaagagcaa gctatccaaa tgcttagtga ag

#cctgtctt  69360

ataaggacct taatcccatt caccatggga ggtattctca tgacgtaatc ac

#ctcttata  69420

ggccccacct cttcatacca tcacattggc cattgtattt caacatctaa at

#tttggagg  69480

ggacatgttc aaatgatagt aacatcttat agctctctag tattgaaata aa

#ccttttga  69540

ctctcttcag agcatgtgat tcacttgaac cagatatact gcccatattt at

#accatccc  69600

aacttgcaag aaattatctg caatttaaaa acaaagacag aaactttctc ca

#ttctgatc  69660

ctatttgcct cattccaagc tcatctttcc atttgccaga taggcctctc ag

#acttctgg  69720

aggttctcaa actcaatgaa tatgaatcca aatttgtcat ctcctaccat ag

#tcttaccc  69780

caccaaaatc agtgtatgtt cctgaatgtg ctgttgtgaa ctgccaaaat tc

#actaatat  69840

taatgcatga gttagctttt actannnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  69900

nnnnnnnnnn nnnnaatttg cacccacatc aaattattcc gagttctttt ta

#ttaatctt  69960

tctatagtga ctctaggttt catctccttc tatctatccc ttctgtcact at

#tcttattt  70020

aggatctcat gttttacttg gccttttgga aagagcttat ctacactcag tg

#tttttcaa  70080

ataacttaac tttttcatag ttacctgagt tgtcttccta aatcaatgga aa

#aataattc  70140

ttttctcaaa aacctcagcg gaatcccact atttaaagaa taaactccaa at

#tctttaac  70200

gtaacaatca ggtactctct agcttaattc ctaaattact acttgggctt gt

#acattttt  70260

attcctccat aaatactggt ctctctgtga tagttctgtt tcttggattt ag

#ggattctg  70320

aatctttttt tgtaccatga atccctttgg aaatctggtg aagtttattg ac

#tccttcct  70380

agagtaattt attttttact tgtaaaataa taacattacc aagataagca at

#tctacagc  70440

aatacaactc tatctgtctg tgggtcctgt tgtttatgtc tttgtggctt tt

#ctatgatc  70500

ctccccttat cacagaagtg cccctaaccc tgctctatcc ttctcatcta tc

#aaagacta  70560

gtgcaattcc caccttcact gtgcatcttt accttgcaac cctgccccat ct

#gtatcatc  70620

actttgctct ctcattgtag attatatgtt atttatttct atttatccct ta

#tctttatc  70680

ttttatgata ccttggtaat tgacaacaat gtggcaaata ctgaactagc ca

#cttttaca  70740

tactttgttt tcataatttc ttttaaagca ctataaaata ttcatatgat gg

#ggaaactg  70800

aggtccaaac attttggtgt ttcaccaaga tcatacaact tgtattcttg ct

#agattatg  70860

agttatttga ggaaaggaat atttcatctt cctgattata tcttctccgg at

#ctgtggga  70920

tagtactttt tcaataatgt ttgcccatat gtatttaaaa attgaaaata tt

#gggaaata  70980

cttatctctc aatatttaat atcattagaa ttgactaggg gccccaggag ga

#cagaggtg  71040

tgtgcagcag tgttcttagg ttaatgtctg tgtgaaatta actgtggcta aa

#tctttccc  71100

tgcatactat tatctctcct aattcccagt ggcttcacaa attgaaattg ta

#cacataag  71160

gattttaaac agaaacattt ttaagatatt gtctcttgtt ttgcgttact ga

#aaaaataa  71220

caaagtagga caaattaaga aagtagaaca aattaaagaa tttaattgga gt

#cataagga  71280

attcaagaaa tatttgaaga ttgcatttct aataatagat cagaacaatt ga

#ataaccat  71340

ttcatattct ccaccctcag tgaaaacaat aaaatcaact caatttacat tt

#gtaaaaca  71400

atatatgtat actttcatta taccagtttt aattttcagc tgtgcttctc ta

#tctctctg  71460

tagtcaaata ctacgtttct gcaagggttt tcttaaaaca gcattgctag gt

#taactgtt  71520

ttaaaataaa aatatttgct taaaaaggtt tactttagca atagtaatgc tt

#tcctttca  71580

aaatattatt tcaagtttta aaataatgaa cttatgattt taattaatta ag

#ttttcatg  71640

tggaagttgt tcatctagag taactaattt taaagaattg gatcttttta tt

#tggtaatg  71700

cctttagcac tctgtgaaat ataaattaat gtaaaattaa aattaattta tt

#atgactat  71760

ccatttttca tggtcttttc tattattgtg ctgtagcaag tactaattct aa

#tgtagcta  71820

gataattaat tttctattgt catagctcca gatggtcctc ctgaaaatgt tc

#atgtagta  71880

gcaacatcac cttttagcat cagcataagc tggagtgaac ctgctgtcat ta

#ctggacca  71940

acatgttatc tgattgatgt caaatcggta aggcatgtct taccttctgt aa

#aaagccag  72000

tataaaatgg ttaataatac aagatttgga accagactat ttgaatttga at

#tttggctc  72060

tgttagacag taggaaaatt actttacttg tttgtgtttc aatgtctaca tc

#tgtaaaga  72120

ttaataatag taaacagggt atgaggactg aatcagttaa catgcataaa ga

#acttggaa  72180

cattccctga gatatggtaa atgctcaata aatataagat attagtaaca tt

#attataat  72240

atgttttatc agtgtataga atgtgtatat atatgtatgt atatatacac ac

#atacaagt  72300

ggttaaattg gtagtaatac aaatatctgg tttacagtac ggtagaaagt aa

#ttcataat  72360

acaaaatgag aagagagaag gcattaggag aaatatcttc cagataaaat aa

#catcagag  72420

ctaagtcttg aaaaataagt gaagcagaac ataaaaggta aatgggaata gg

#ggtgtaaa  72480

aaagtaagcc tcattcaaga aaaatcaagt cttttggcaa tcccaggcta ta

#gcattaaa  72540

aaaaataagt tctaagagat gaggctagat ccagaggctg actatacaac aa

#gttacaga  72600

gttagggttt tatgttaagg gcagtggggt gccagttggt gatacagatt tc

#tactgtat  72660

aacataggca tggttgcagt gtagaggata gattgagagc agtatggggg gt

#gtaaaatc  72720

aggcaaggag acaggaaact ataaaagggc aaggaaaaag aacagaggaa at

#gtagcaag  72780

agaacgaatg aaaaataatc taaacctata gaatttggtg aaaaatcaac ta

#actcatga  72840

tggtgagtga gtaggataat taaggatgat tgtaagttat atgacagaag at

#tatgagga  72900

ggaacagatc tgtagaggaa aggaatgagt tcagtattag acacactgag tt

#tgaaatat  72960

gtggcagtcc tccaggtcaa tacacccatt ggcagtatta aatatggatc tg

#gagctcag  73020

gagagaaatt ctggatttcc agatttgggt aatgttagta tttagaagat ag

#tcaaaatt  73080

ataagagtga atgagattca ctatggaatg tgcaaagtaa gatgacaacc ta

#aggacagc  73140

accctgggga ctatcaacac ttaaataaga ggccattgaa gagactgaat gg

#gagtagat  73200

agccatttgg atggaaatcc aggtatgaaa gtcaaaccct tcatataaga ta

#ggatgctc  73260

aatgatgtca ataatgcaga actgttagcc agaataaaga ctggaagtat tt

#cctttgca  73320

ccctgcttgg gttttgctgg gcctgatgaa cacagttttc taatagcatc tt

#atgcatta  73380

aattgtatag catagtgatt tctcctcctt tctctctgtc tcttgaccaa ct

#ttataatt  73440

ttattatgtc tttgtagtat tccttaaatg gagatataat gcttctatct ca

#aaagacct  73500

gtcatcatta aaataaaatt gaaagaattg ttgatgtatt tgttctccaa tt

#cagtctct  73560

attttctgtt ccttttgtag agcatatttg tgaagatttt agtatgtaat ta

#gccaaaaa  73620

taattagcac gaatgatgaa tgccctggga atatgcatta aaaacaaatt at

#aaaatgat  73680

aaagctttac tctgtcaaat gaaaggcact ttattaatga aaatagttct cc

#cttggaaa  73740

ttctgcttaa aggaacaaaa aaaataaaac atattaagaa gtgattttgt aa

#tctcattc  73800

ttgtagcctt cttgctgagt ttcaaagtga gcaagggaaa gagggtagaa tg

#ggaagata  73860

acaaatattt taattgctta ttttctacaa gttacatgtt catctcttcc ta

#ttcattat  73920

cttattatat gttatccagc aatattttca tataaacatt attaccttca tt

#ttgcataa  73980

aagaaatcta aggaacagag acggtgaata acttctcaca gagctaagac tt

#ggctaaaa  74040

ctaacagatc aacaatggtt tgacaggaga aagggaaggt cattaaataa ca

#aataaaat  74100

tcgccaacat aaatatagcc tcaccaaaac tcctttagat caacaatagc aa

#gaacagat  74160

tcagatagaa cctagtaatt cacagtttct ttaggattct tggaattaag ta

#gaggtatt  74220

tccttccttc cttccttcct tccttccttc ctccctccca ccctccctcc tt

#cccttact  74280

tccttccttc cttcctcaca cattttaaaa tacttccaat gtaccagaac tg

#tgctaggt  74340

gctggatatg tagaagtgaa caactctgat aaagcacttc tgcagctcat tt

#cattaata  74400

tgtaattatc aacattcaag aatcactggt tcccagacta aggaagaata ca

#tagcattg  74460

cacttgggac ttattgtgca gcctgtcttc tacaaaatac tagccacacc ga

#taaaactc  74520

ttaactttaa aagtgtaaac aaaaattgtc aatcttaata aaatgtaaat at

#catcctag  74580

atgattctgc tggaaattaa tgcatttagg atcattttca ttgttccttt ta

#ctctttga  74640

ctgaacaaat tgttatgagt agcttctgtg catcaagaca aggatgaaaa ac

#ataaagct  74700

ctgctgtcag gtagctcaca ccctagtggt acacactgga atagaaacag ct

#aattatag  74760

agtttgaggt atgaagtttt gtggggaata taaggtaaca aaataattaa ag

#ttcctgtt  74820

atgggaggaa tagaagataa aggagacttc atagagcagg ttaaatatat tc

#taggactt  74880

gacgaatgaa taggggtaca caagatggga aagggtggag gcggggtagg aa

#gagaaaaa  74940

aaataggaag tggggcagga agaggagaac cagagtctaa ttgctgataa tg

#aatataaa  75000

gtaacacttc aaaaatgatg aaagacattt tataacaata gattatcaag ta

#caatatga  75060

gcaaacaaac ttggaattga ttgaaggaaa atgagtcaga aagatgtgat tt

#cagtcctg  75120

ggtaagtgga caagtaatag ctaacaacaa caaaggtgta gtaggtttaa ta

#aagaaagg  75180

taatgatgat cttttgtact gccagatttg aggaccgaga attcctttca aa

#ttttgtta  75240

aatactttta aagatataat atatgcgtgc catgtcatat tttgcgactt ga

#tatttgtt  75300

atctattgtt ttaatggaag gcattggaag agtataattt ataaattata ct

#tataaatt  75360

ataaatttat aatttataaa ttataaatag ttaaatttat aagatcaaac cc

#agtgacct  75420

tgtggaagtg tagaattcta tggactcttg aaagacctgg gctcaaatcc tt

#cctctgct  75480

actaagtaac actgaggaag tcaccttacc tctataaaat cagaattcaa at

#agctataa  75540

aagacaagtg acatgaacca gtagtcaaag tgaagccaat tgagtggggc tc

#caatgaat  75600

gggacccagg cccctttaca gaggtcaata gttacccatc tctgcacctc tc

#aataatat  75660

tttttcagca tgaaattaag cctagtctta aggaaaatta caaaaagcat at

#ttttatgt  75720

gatattcaaa tgttaacaac tagttaaata cacattttct gccagtggca ta

#tattcctg  75780

atcaatagga tttctacgct gatttgtttt tcttccattt tcgagaagtg gg

#gcatttct  75840

gtccactgct ctgtcttaag gtgggaatga tctatttgac tgtatgcaac ga

#tagtatta  75900

tttatatcat ccttttacta tgtttctttt ttttcttttt ttatagcaac at

#cttttttt  75960

taaaaaaaaa ttgagttaat tttatttaca ttacctcagc aaacatctct at

#aaatgagt  76020

ttccaggaca acatttacaa tatagttata ccatatgcaa atcaatgtgt gt

#ttcgccat  76080

attatcaata aaatatgttc ttagcaaaga gcattaaaag aatacattga ac

#caaccaac  76140

caaacaaaaa atatttcaaa gttataaggg aaggtcaagt tgaaaatgga ct

#taatagtg  76200

ttcactgtgt ataaaacctg gttttaagtg tttcaattaa gatacctgaa ag

#tagtatgt  76260

atgataggat tttgaatttt ctcatggtta tcttgggaaa agcccttcta ct

#tagtgcta  76320

gcaagtttag ttatgtttaa tatctggagt gaataggcca gaacctccat aa

#aggacaga  76380

ctatgtttga acaaatcata tagctacatt tcatatgcct aaagacactc at

#ttatgcac  76440

attaataatt atgacatcca caattaatta ctatccagtg ctacacatag ta

#ctaaatca  76500

gagtttttca aactgcagcc atcatcagaa tttttaaatg aggtggaata aa

#gttaaaaa  76560

gagcagaaaa tatcaaagtg tacttcagaa agatggtgta tttctgaaaa at

#gtgttaca  76620

gtcataagat acgtatatat tttatatcta tcagtcttat cttctgaatt at

#gttacaaa  76680

gagtgtttcc ttttgtgggt aatggtgaaa aaatattgaa atctatgtgc ca

#actacttt  76740

agatttgtcc tttctaaatc tcagtgaaac actgtaaata gttgttatta gc

#ccaatttt  76800

acatgcattg aaagactaga ctgtgtaggt ggtttctcaa atactacagg ag

#ctataagt  76860

ggccaatttg ggatttgagg cctgtgtgat taggtaccaa aacctctatg ct

#ttcttcta  76920

caaaatattg gagtcaaaag tagagtttca ttgactgcaa agatgatttt tg

#cttattta  76980

tttaatgggt tggttaatca cggttggctg gtttgtcttt tttcttttac tt

#tcaactat  77040

taaaataatt aataattagt aagctgttat aatagcactt tagatttccc ag

#agcaatct  77100

gattgtaaaa taaataaata caaatttggc tagataaata catctcacgt ag

#ctttgtat  77160

tattatgttt tggtgactca gatttcaagt gctgcttcct taatttttac tt

#atattttc  77220

ctataggtag ataatgatga atttaatata tccttcatca agtcaaatga ag

#aaaataaa  77280

accatagaaa ttaaagattt agaaatattc acaaggtatt ctgtagtgat ca

#ctgcattt  77340

actgggaaca ttagtgctgc atatgtagaa gggaagtcaa gtgctgaaat ga

#ttgttact  77400

actttagaat caggtaagga gaatttctca accttgctaa aaattgactg ag

#atttagct  77460

ggctttctta cagttcatca tactccacca aaaaaggata tgtgttatga ga

#agttttta  77520

aagcatataa acaaaaaaat tagtgactct ctgcaactga caaaaaggaa ga

#tttctatt  77580

tatatttttg aggtaaagag gagttatgta gaatattcaa tccttgtaaa ta

#cagcaaca  77640

attaaaggta tccgctgtat ttctttgcac ttatttaatc tgctagttgt tt

#cagaaatt  77700

aagtaagctt gcctaagaga taatatttcc aactgtctat atccaataac ac

#ttcaacta  77760

aaattctatt tcaattattt ctgtcccatt atttgaatga atattaaact tg

#taatactc  77820

tgtgagtatt aaataatctg gaattcgaaa gtagaatcca gctcacattt at

#cacagctg  77880

ttgtcttccc ttagtcaagt aatatatgtc ttattatata actctttgat aa

#atgtcaga  77940

atacatacag atttcctcaa gttcttatga acagggtctg aatgaataat aa

#gattgtaa  78000

ccaataagaa ataaatttgg aaatcaacat cccagaactt gcttgcccca tc

#ctccttca  78060

gactcctgat gttctttgcc accagatata tcattggaaa aagcagatga ag

#ggatatgt  78120

tgctatagtt tatttgttgc tatctgtaag gtaagtatag gaagtaaaat tt

#ttttacag  78180

ctagtttttt tcgttacatt atattctcta tgcattttgt ctgtaaagtt at

#ggttctaa  78240

attaaaaggt aaattttatt atcagcatcc taaaattcca tttgttccta tt

#cgtctgcc  78300

aagtatcaca ggtatctatt ttctgattat gctttttact tctcaatccc tc

#ctacctgt  78360

gaggaaagat atgatgaatg tactcacatt tataccataa agcattgttt gt

#caaatctt  78420

aatgtgctat ctgtttcaag gatatcacaa tttaatacat ttttactaaa tc

#tctaagag  78480

tagaatttta tgtgtataac caaaaatctg ggtactagga aattttttac aa

#cattgaga  78540

gaattccttg gtttatctga cttaaaatca catcctaaat ttagagaaac at

#ctcataag  78600

aaaatatatt tatgacacag cataaaaacg tgtagtaaca aatgcaaaaa ta

#tctctctt  78660

gaaccaactt aacctttatt ttagctttgc atttttccat ttaaaatgaa at

#atttgaca  78720

caatagtacg tttatctgct tctctctctt ttattctttg ctgttaattt at

#ttacattt  78780

tttgcaagat aatgaagctt gaatatctga actgttgaca gccaaatatt ac

#atttcttc  78840

atggaaattc tttacttagt atggaaggat ataactattt caagttgaac aa

#aatagata  78900

tagtcattca atcagtcact tatataaaga acactaatta tgttatgcat ca

#agaagtag  78960

ctcctttatt cataaaataa cttttatcct catacatatt ttaataatgt at

#tggtgctt  79020

agcttgtcat atgttaagct gttatttatc taaaataaac taaaatattt at

#actatata  79080

aagaactctt aaaacccagc aatttaaaaa aatccaatta gaaaattggc ag

#aagacatg  79140

aacagacatt tcaccacaaa ggagacattg ttggtaaaac aaacaaacaa ac

#aaaaaatg  79200

acaacataag ttttcaatac cattagccat tcggaaaatg caaattaaag cc

#acaataag  79260

gtattattgt ctatgtacta gaacagataa gataataatt taaaatatgg cg

#atgatacc  79320

caatgctggc aagggatgca gaaagactgg acctctcata cattgctagt ag

#gaatgtaa  79380

aatggaatag ccacaatgga aaataatttt gcagccactt ataaaactaa ac

#atgaaatt  79440

actgtgtgac ccagtagtca cactcttggg cactgatcac agagaattga aa

#aattatgc  79500

tcacacaaac atctgtcacc aagattgatt gcagttttat tagtaataat ga

#aaactgga  79560

aacaacccaa atgtttttcc atgattaaac aaactctgct acatccacaa aa

#tggaacac  79620

tactcggcaa taaaaagaag aatgtactat taatacatgt agtatccacc tt

#gatggacc  79680

tcaaggccat tgtgctattg tcattttcaa aatgacaaaa ctatacagag ga

#agagttat  79740

tagtgttgtg aagaagcata tactgcaaat aaaagaggat actaggagag ag

#tttcttta  79800

gggtgatgta atagttttat atcttgttta cggtggtggt taaaggaatc ta

#tacagaag  79860

acaaaattgc ataaaagtat aacaaaacat ggaaaggggt gaaattggga ta

#aagtctgt  79920

agcattaaca gtattgtacc aatatcagtt tcctggtttc atataaactc ca

#gttacata  79980

agatattacc aatggggaaa actgggagaa agttaaatgg tatgtcctgt tt

#ttgccact  80040

tcttttgact ctaaattgtc atgccattgc actccagtca gggtgacaaa gg

#gagaccct  80100

gtctcgaaaa caaacaaaag attaaaatgt catagaaaca cattctgtgt aa

#aaataagt  80160

gcatataaaa caaacaaact ggatcaccat tatggttgct gtgaattaca ga

#tgtcaatg  80220

attctataat gcgtcagtca ttttgctagt ttttgcagtt tatatggttt aa

#tttgtgga  80280

atactcttta aaaacagaag ttcaaagcaa ataaatttat gtggagataa aa

#aggaatac  80340

aatattttta aaaattaatt gttaaatatt ttattttagc cccaaaggac cc

#acctaaca  80400

acatgacatt tcagaagata ccagatgaag ttacaaaatt tcaattaacg tt

#ccttcctc  80460

cttctcaacc taatggaaat atccaagtat atcaagctct ggtttaccga ga

#agatgatc  80520

ctactgctgt ccagattcac aacctcagta ttatacagaa aaccaacaca tt

#cgtcattg  80580

caatgctaga aggactaaaa ggtggacata catacaatat cagtgtaaga at

#ccgtagct  80640

tcagttaatt acccaaatga caatgtcagt ttatgaactt ggcatttaaa aa

#tattgcag  80700

tttgtgtaca catgacattt cccatatctt tttgtgagat tgtttgacat ct

#caacaaaa  80760

ataaattttg agaactgaaa ttacctattt tctgctataa tacaagtact at

#taaattaa  80820

aatatgtaaa taaccaagaa gtttgcacaa taatagtaga aactcagaca ta

#aaagagaa  80880

agaaaatgca cattaaaagt aaaagaacag tgatatataa agagataact ct

#gcctaaaa  80940

aaaagctata tgattatgaa tttaaaatgg aaaagcaaat tttaaggaca aa

#agacagaa  81000

ataattgttt acctgtttaa aattctcatg cattttaacc aagtattact aa

#aagctaat  81060

agcattttat gtcttaattc taaattccct atatttggac agaaatgtat gc

#atgagttc  81120

atatacatac acagagacat atacagacac aatttgtttt attccttgcc ta

#cttttaga  81180

tcatcttgaa attttcaaat aaaattatat ggttcagaga aatcatcttc ta

#agaaacag  81240

aatttactct aaatcttcaa gtagtttagt aatctgccta ccaactcttg at

#taatatca  81300

atgtaattat caggtcattg ataataattt gtatatgtat atgtgtataa aa

#tgaatata  81360

tttactactt ctctcagtca ctttgacttt atatctttat aaaataaatc tt

#ttggggat  81420

tctttttgca tgtcccatta agtggaccac cattgtgaaa gatcgattag ag

#ggaagacg  81480

gtgactaaga gaataaagta acctgggttc aaatactggc gtctgtgcca gg

#gcagcttt  81540

atcaaatctg agcccagttt tcttatgtga aaaattggta ataggaataa ta

#acttcttt  81600

ataggatatt tgtgaggatt aaatattcat agttatggta ggtagtgaat gg

#tacatata  81660

ttttggctat tggaagagag aaaataggaa ccaaaaatgt gcaactaatt aa

#taattttt  81720

aaaaatcctg tttgcagact gcaatttgca gtatccttaa aaccttgaag tt

#tgttagga  81780

tgtataactt tagcacctgt attgacctac tgaatcaaaa tctgcatatt tg

#catttaac  81840

aaggtgtgcg aatgacttct ttgcacactg caatttcaga ggcagtgccc aa

#attttcac  81900

ttattatttc acttaaatgt tagattctac cataaagaaa taaaaataat gg

#accatact  81960

ataacatacg ttttttattt ttaatacttt ttttttgaat ttacaaactc aa

#atattttc  82020

tctaggcatt ttcaagccac attttatggt cttggtttat ttatcatatc ta

#ccacatgt  82080

atattgtaat attaactcaa taaaaacatt ttaaaagtat ctagagtgtg gg

#agggtggg  82140

ccactaatat ataaaacaaa ttaagtaatt ccaataaaca tttcgtatac tg

#aattgggt  82200

ttatcgagta caatgaaata agccaagaat atagtctctg tttaatatag ca

#agataaag  82260

tgaagaaaag aagattctct gtctacagct tccttagcac aaagttaatt ga

#aaggattc  82320

acgtttgtgt aaatcaccct ctgtgtatac aaacagaaat gttttatgtg ta

#tatgctgc  82380

attatgcagg ttatagcgtc agatactttg gggcaggggt gaagaagggc at

#aaaggcca  82440

tctttcaggg gaatgattta atacaggaaa actgggaggc tgaaggaata gg

#atttcatc  82500

ttagagaggg aaaaaaagag gatcttgaat atggatttac agaaggccag ag

#ttacctag  82560

ctacagagag aaggaacaga tgttagagta gatgaaggga gagcgtagat ac

#agtggctg  82620

tagtgctctg ttcttcctac attcacatta aaatcatggt cagtccaggt ct

#cagtgata  82680

gggctgttta gatcactcag cctttgttct cagcgtttag taccagaaca tc

#aattttta  82740

gaaatacttc attgttaatg ttcttcctac atatattata ttcaagtgca ag

#aaaataca  82800

attaatagac tatatgcagt tgttttttaa agaattattt aaaattacat gt

#taccataa  82860

tcagttttat atatatatat ataactatat atatacatac atatatagat ac

#atgcatat  82920

atatatatat acacacacat acataagcaa tcacttgaaa atagtaacaa at

#atttgttt  82980

gttttaggtt tacgcagtca atagtgctgg tgcaggtcca aaggttccga tg

#agaataac  83040

catggatatc aaaggtacat acatgagcta ccttcctatg aaatgctatt aa

#tcagtgat  83100

tataatttaa attccatact tgaaataagg atgtagacaa gcctttaagt ga

#taaatatg  83160

catatattaa gcacatacta agtaaaaatg tgtggttatt aaagctatag tt

#aaaaacgt  83220

ttaaatgatg atgtgaatta ccatgttaaa taaggagaat gtctttattt ta

#tatttctt  83280

taatatattt tatatttcta agtttaaatt tttaaagaca aattttatag tc

#cgttattt  83340

gatgtttctt taaatgttat cgaaaagaaa gtgtgtttaa attgcctatc at

#tagacttt  83400

gactaggtct aattagatta ttagattgtt tgactgattt ttattttgga ag

#tgagattc  83460

tttcagttaa ttaaatagtg ttttttgaac taccatgtag gttggtttat ga

#ttctgaca  83520

gtaaagtaaa tctatcaatt catgattctg gcaagtattt tttatagaaa at

#ataactaa  83580

aatttgggtc atcctttcaa aataaaacaa acaaacaaac catggaaacc nn

#nnnnnnnn  83640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  83700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  83760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  83820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn  83880

nnnnnnnnnn nnnntgatta aaggtgacag cattaggctg ggtttagggt ca

#aactgctg  83940

gtattgaatc atggattagc cacttgatat ctatttgact ttgagaaagt tt

#cttaacct  84000

ctataggcct gaaaaatgga gataatacta gttcctatct caaagttact aa

#ctttgagg  84060

attaaaagag aatctgaaaa aacccttaag acccctgcct ggaaacatgt ac

#tattaaga  84120

ataaaaaata ttcactgtta gatgaaactg ataaatttta tctttttgtc aa

#tggattat  84180

ttatataatt gtgatactct ttataagtgt ttgatttaaa ttgataaatt at

#ttactttg  84240

ggaagtacaa atgcataagg tctttaaaaa catcaattat ttcacaaaat ga

#cttcacaa  84300

agaatagtat gagtcatacc ttttaaattt atctttgtta ttcagtggca ga

#cgtgtagg  84360

tgtacagata gcaatacatg catcactttt atgctttagt aaaacttccc at

#ttcagatg  84420

ctattcccat ccccatgcca ggttggttaa tcttgacttt tactccttac ct

#aaggcagg  84480

cagacttacg acttctagtc tagagaagat atatgaacaa tgtcttagtg gt

#gggatcct  84540

agagaaatta tgttttctct actaatacac attattttat taatgatgtg ga

#atattcaa  84600

ttggtagtag tctcatgaaa tttgcatttg acagatatta tgaagttgga ta

#gtaatgtc  84660

caatgctgga ttcctctaac tggaaatctt ttcctgggga ctcccaagtg gc

#ctggctga  84720

gaatctcaga actacctgaa acctgagcct cttcctactg aatcctgcct tt

#catcttcc  84780

cttttacagg tgtcatacct gtgatctgtg gcacttactc cttccctctg tc

#ccttaatt  84840

cttcacagat aattccccca aaaaacctct tacacatcca gtctcatttt ag

#cctttatt  84900

tctcttagta cccaaactga tacattgagt aagaaatttg catttgaatc tg

#atgctcaa  84960

aaaagagtga actacagaac cgagtcaaaa aaatgtgatt caaatgttga ta

#gttatctt  85020

tgataactgt atgacataag ctcttttccc tatctacaaa ataaaaacaa tg

#ttgataat  85080

cctgtgggat tattgagaag ctttttaaaa aagattctta aaaatatgga aa

#ttgaaatt  85140

tggttaagta acttactgat gctcaagcac tacattcaca cacacacaca ca

#cacacaca  85200

cacacacaca cacacacaca gtgagctata agaccaaatg aaacaagcag ag

#cttctata  85260

catatatttt ggagatattt gtatgcattg atgacaaatt atgaattatc at

#ggctcttc  85320

tagaagttat tctgttaatc tgttaaggtt aaaagtacat atatcttttg tt

#ctagaaat  85380

tctgctctta ggaatctatt tcatagagac aaaaagatca gtaaataaag ct

#cattgtaa  85440

aacaaagcta attattgtaa aacatgttta aaaactggag gaaaagtgaa tt

#cccatcac  85500

cagatgaacg gttgaataaa tatgatgcag ctgcaccatg atatataata ta

#gtcattaa  85560

gaagaatgag ttagctctgt acctggtcac ttggaaacat ttaaccaagt ta

#tatttaag  85620

caaaaaaagc aatatgaggg gtaattataa tacatactag agccccatta ga

#aaaagcaa  85680

acagtgagaa gagtgtgttg tgcatgacta tttatgtatt tttatatgat ta

#tgtgaaca  85740

tggaaaaaaa tatgggatgc tatcatctag atgtttagca tggattaact gt

#gggagggg  85800

tgctagtatg actagaacag aaattagtaa accagctaaa agaagcaaaa ga

#aactacac  85860

taaaattata gtaaaaagta aatatgttta tgcatttata aaaaatatat at

#gagtgaga  85920

gcatgtataa attgaatttt taatatgcaa agaggagtca ggtgcctgaa aa

#tcacacct  85980

gggctctatt atctcaggga agtctcaaat gtataattat gctttcagga ga

#taataatt  86040

gctaaatgtc tgctgtctcc aagcacaatt ctctataatt tgttatttat gt

#ttgtcatc  86100

actttatctg taaattaact gcatgaaagg agaaagattt tttcttttgt tg

#atgaaaca  86160

ataacatcaa ttgcagtgat atttcttctt tgtttatagc tccagcacga cc

#aaaaacca  86220

aaccaacccc tatttatgat gccacaggaa aactgcttgt gacttcaaca ac

#aattacaa  86280

tcagaatgcc aatatgttac tacagtgatg atcatggacc aataaaaaat gt

#acaagtgc  86340

ttgtgacaga aacaggaggt atcatcacat gtcaatttat cttgttaaat tg

#tggagtgt  86400

agattactga gtgctaaaaa gaatttagtt caaattaaga ttgactccca gt

#tatcacac  86460

acctcttgag attaatagat tgtcaatatt attattaatg ataccaatca tt

#tctttgta  86520

aataaataaa ttatttattt attttatttg taaaactttt ataaatgtca tt

#tatttata  86580

ccaattattt attctttttt acctatcaag aaaggcacag ttaaaatatg tg

#atttatta  86640

attccatata ctagtagata aacatgtttt gattttggta agatggaatc tt

#gatagctt  86700

ctttggaggg gtgaacaagt gagttactct tgattgaggg atgctctttc tc

#tacctgat  86760

aaatcatcct ttataacagt tcctgtagat tcacatgtaa cagagaagaa ca

#gggttacc  86820

tgcctataca ggtggatcac ttgaattatc tctggtgact gatgttgcat cg

#agagtccc  86880

cttatacaat tataaaaaca ctatttataa ttgtaaaaat atattcatat gt

#tacttgga  86940

attattgttc tctttgtttc tgaaaacagc tcagcatgat ggaaatgtaa ca

#aagtggta  87000

tgatgcatat tttaataaag caaggccata ttttacaaat gaaggctttc ct

#aaccctcc  87060

atgtacagaa ggaaagacaa agtttagtgg caatgaagaa atctacatca ta

#ggtgctga  87120

taatgcatgc atgattcctg gcaatgaaga caaaatttgc aatggaccac tg

#aaaccaaa  87180

aaagcaatac ttgtaagtat aggttatatc taccatgcat tctgttagca ag

#ctagttag  87240

tatctttcat ccatccatct gcctgtcctt tcatctttcc aataagcact gg

#atgtccgc  87300

cacgtatagt gacctgattt ttctggcact aggaatagaa agataaactg aa

#aattattc  87360

ttacattcca taaacataca gtattatagg ggaagcaggc aaccctaaga gt

#aattatga  87420

tttgatataa gttacataaa gaccatatga aaaatgtgct attggagaac at

#aggtatat  87480

aggagaattt aattctttct gtagaggaca atgtgactaa tgtgtttttc ac

#ttattatt  87540

ttaccatcca tgctgatgta caggattttt gaaacactat cctatccttt ga

#tttaacag  87600

tggcttccct ttatgtcact cataacaata actctctgct ttttatctca tg

#aatgagtg  87660

atagaaatat ttaataccac ctttaatatt tagctttttg tagcccctaa aa

#acccaaca  87720

ttttaaaatc aatttgatat tttggctgta ttaaattatt tgctaaattg at

#tatcttcc  87780

ttttgaattg attatgttat ttttgtattg taagactaca atttttaaaa ga

#atcatctt  87840

atccttgtgt gattttcaaa atataatttt tactagtaat tttttaaatg ca

#ggtgcttt  87900

catttgtgcc tgttagttaa aacattatca aattctttac aaatatccta ag

#ccaagtta  87960

acattggaaa aattagagaa attaggcaaa taaaaataat gctttatcat ct

#ctattaaa  88020

tgcaattact ttggttcaaa ttctaggtta ttgcctgaat agctatacac at

#atgatagt  88080

tataaaaatg atatactacc aagtatcatg tttattcata tttatagttt at

#ttattttg  88140

catatttgtt cctgaaacag actcttcata taacaaataa aatcataaga at

#tttataat  88200

ggtagaggtt caatcatgta ttgcaacgta ttggttttat gtttttaaat gc

#ccttgtgc  88260

ctttattttt aaattaagta aatttcaatt gtctctgagg atcttagatt ct

#ttttgtaa  88320

tttttaagct tgatcttctt ctgtatcctt tacttcaaat gctatggaag ca

#aaaaagta  88380

tacaaatgca actgtgcaca cacagaaata acaaacattt tcttaatgtg tt

#tatatgtg  88440

aacaagacaa gttctatatc atcattttaa tctaattcac tagcatttgc aa

#aagtgatt  88500

gaggtataac agttatgcct tttatttata aattatgtta gtgtaacacc ct

#tcacagat  88560

atcaaatcat tccatctaaa caaatccttg aaggaggtga gctgattcag tt

#gttcaaac  88620

tgctaactgc tcacgagttt accaaatttt tagcccctgc ctcatcaaat tc

#aatgggtc  88680

aaagtacgag ataattattt gtctcatata aatatagcat atatttctcc tg

#atgatgat  88740

tccattccaa attttcatct tgtaaattca ttttcttttg aattaaataa at

#agttttta  88800

taattacttc ttgagttatt cataggaaaa atcacatgat atgcaaagtg tt

#gatttttc  88860

tttttttatt ttatagattt aaatttagag ctacaaatat tatgggacaa tt

#tactgact  88920

ctgattattc tgaccctgtt aagactttag gtaagacatt tttgtaattc at

#ttataatc  88980

tcaacatatt tatcaaagtt ggaacattta ttagtaaatg tattaatcca tg

#tctagatg  89040

ttttaaaata taaactcatt taaatgttaa ttagcctctc tagtaatatt tg

#tgggtttt  89100

taaaattttt tcttttaggt ttaggagtac ctgtgaaggt ttgttacaca aa

#catctgtc  89160

atctcatctt aactatcctt taagttaggt cagtgcttct cagagggatt tt

#atacccca  89220

ggggatattt ggcaaagtct ggagccattt ttggctgcca taacaggatg gt

#agtggtgg  89280

tggtgcatgc tactggcatc tagtgggcaa agattaggaa tgctgctaaa tt

#tccacaat  89340

gcacaaaaca gcccgtaatg tcagtggtcc tgaggatgag aaactctgac tt

#aagcccta  89400

atgttgactc cattttacag atgaggaaac caacacccag attctttcag ta

#tttaagtg  89460

gctaggccag gattccaaca ttacagaaca ggatttcata acattacatt ac

#aaatatgg  89520

gatttagacc tgggttcaaa tcttggctct gtcacttgag aaaataattt aa

#tttctata  89580

aatctgagtt tcctttgttg ggaaaatatt gataagagta tcatccttga gg

#ggttgttg  89640

aagttttgtg taaaacaaca tatataaata tattaatatt ttatagttag ta

#aattttta  89700

aagtttaata gcttttttgg ataggttata ataaaatatt ttagaaacat tt

#ttatttag  89760

gagaaattat ttctctagaa tttcactgag aggatcacaa cattctacat tg

#tttgtgcc  89820

aggccctcaa aagccccagt ttattcgtct taaagaattg catgaacagg gt

#atttctgg  89880

ggcaccactt gaaaatgtaa gacttcatgt gttgcccaga tcctggcgag ct

#gttgctca  89940

gtgtatcttg aactgctaat agacttcagt gagagttatg actggagaaa ga

#cggattgt  90000

cccaccattt ttagccagaa attctcattg ggttatggaa atactaattg ta

#taaaaagc  90060

cagcctccac agcctctaca tgtagtcaag gaaactttgc atcttgaaga aa

#tagagggg  90120

gcatgtagtt tgctacatag atgtttgtag agaaataaca aatttctttg gc

#taaaatgt  90180

ttgtttaatt ttatacaaat cattggtttg attaatttta cccaataatt tc

#atcatttt  90240

aaagctagct gattagtttt gtggttttga aattgtatca agtgtttctt ca

#tttgatag  90300

gtgagtctat cacactctga tgccaccaca gtaaaataaa tgtcttcttg tc

#atcagcat  90360

aatttcctat aggttacagc attcataagc cattacttca gctaagtagt ga

#tcctggtg  90420

catttgccaa tggaaggtaa aagacctaga caagatagat aacccatgtg tc

#ttaggaga  90480

taatatttta taggagcagt gctgaaagga gctagccttg ctgtattgta tg

#atgttgtc  90540

tttcatcaac ttactggttt catacagatt attcatggga aggcaacatg tt

#ccgtcagt  90600

tatctgagag gcaaagttga gacattcagg gtaatggaaa tgagaaagaa aa

#gctataaa  90660

agggggggag cgccaagcat caggaaccac agtgcacagg agcatgattc ct

#tagattct  90720

gctaaatggc ttctctctgc ccaatgatgg cctcatccag cactataagt aa

#tctcaaag  90780

agctcctcag caatggtctt ctctttcttc tttccactca cagtcaaggt gg

#tggaatac  90840

aaccattaat cctggaatgt agcagaaata cacagtcagg tttttgattc ct

#tctttgga  90900

agtataacca ctgccacccc aatcatctag gtatgaatct ttgtgtgctc tg

#gaaacaga  90960

aggagtctac agtgagtaaa agatgtgtaa tgaaggacag agcacaggat cc

#tgccaaga  91020

ctaaggaagg agggactggt gaaatgtaga ctggacacaa tatatagaaa gg

#cactgggc  91080

tgctgaggga tagtgacaag gaagggtcct atgtgtctat gatcaaatta ct

#cagagttc  91140

agtgtatttt ttgtagacca gtgacatgat gacagtcttt taggttgctc ct

#tagagtga  91200

tcttccaggg actctctcct gagatacagc agctttgtta attggccttt gc

#cctacagt  91260

gcttattctg gattgaccac atggagttct gctatttaga tagtcattta ta

#gcgagtaa  91320

gacagtgaca agtggaatca aaggaacttg cttggtttgt aatcgttagt tg

#tagtgaaa  91380

tgagaatgca cccctggagc agaattcctc aatgactagc aaagcagccc ag

#ccatttct  91440

ctggttaatg gatttaacac catcaattac tgttgtcata tttgctttct ac

#cagactat  91500

tgagtgtcgt ggttctatgg agattagagt cactttcata ggtcaagaaa ca

#aacaacat  91560

cccctagaag tggtcctggt ccatttgaca ttgatagaac gctgtagatc ag

#gggtgtcc  91620

aatctattgg cttttctagg ccacattgga ataagaagaa ttgtcttgga ct

#acatataa  91680

aatacatcaa cactaacaat agccgatgaa ccaaaaaaaa ttgtaaaacc at

#atcctaat  91740

gttttaagaa agtttacaaa tttctgttgg cccacgtcca aagttgtcct gg

#gccacatg  91800

cagcccatga gctgcgagtt ggacaagctc gccacagatt caaaagagtt ct

#tagtaaaa  91860

gaacattgcc agggaagaaa ctctagaaga actcaaaaag aaaacaaagt tg

#atcaattc  91920

tccaatggtt agtggcaaga atggtttcat ggttgtgaga atgaaggcag gc

#aaataata  91980

aagtgcattc atataatacc cctggtgtca tcagaagatg acaaagggtg at

#tgagtcct  92040

ttttttctct caaatatgtc atgtgttggg atatatgaat catttgcagc ag

#gctaggga  92100

ctcaaacatt cctggtaagc tgctgaagac atatgtgtgc atgtaatccc ag

#actacaga  92160

gagaagtcta ggtcccatca aggtcatcca cccaccaggg gataagcatt ca

#ttcactgg  92220

tattttgcac accacaggca atgagcacta agccgagttg cctgtctgtt ga

#aactttgg  92280

gatttaagag cttttgcacg actctgtttc cacagaccat tgtagtggta at

#tatgcctc  92340

tcagagacgt tattatttgg agtttaaaat taggggcaaa agaatcacca ta

#gactgata  92400

atcttaaaaa tgtttaagtt tagtgaaagg gactaatgaa agtacaagtg ag

#agatggcc  92460

aggtagaact tcactggatg gataagtatg agtctgtgga agagcagttt gc

#atttaggg  92520

aaacctttct ggcctgtagg gataaacagg gaagataacg tatgcattat tt

#taatccta  92580

aataaatact tgaaacttat ttgatttcgt ttttactcaa gattgagtat tg

#gcattttt  92640

attatcaaaa ttcacaaaaa accctcttaa actttttgaa aaaatcttcc ct

#aggcacat  92700

cagtttatgg aaagtgcttg taggcaatgt tttgattaca aggtttaatt at

#agagggat  92760

cctgtgattt gaaaaccaga cacccgtttc tgtaccttac agggctctca tt

#aaagctga  92820

acatgatgaa atcttaaacc ccatggcaaa ggcactctgt gattgttttc tt

#ttgtcata  92880

acacttctca tttaattact atgctaacaa tgaaaagttc caatgtgctc ac

#ttagattc  92940

agaaataggg agttgctatg tatcttttgc atccaaagga ttacttccct aa

#agtcacca  93000

gaggaacaga ggaagattgt attttgttaa cgagacagtg gtaatgtggt gg

#tgaacctc  93060

acatactctg tagtcaagac agacgtattt caggcaggct tggtatatat tg

#aatttatg  93120

agattgtggg ttagttactt aaaaaattat ttttaagttc tgaaatctta tt

#tctaaaat  93180

gagaatacta atactccatt ttagaagcta actaggagat taaatcagat ga

#ataaaatg  93240

gatgaataaa tatgaaatgt ttgttaataa agatacctgt cattgtttat gt

#accaagtc  93300

tttaaggggt tttacatata aactcatgca tcttcactgc aactctgtaa ca

#acacctcc  93360

tatttacata gcgccaattt ctaggtaaga agtttgaagc attgtgtttt tt

#actaactt  93420

gaccaagctt tttaaccatc ccaaaggtgg tggcagaacc tgctttcaga cc

#caggcagc  93480

gtgacctcag tcagtgctgt acttgtaacc actgcacaca ctacctgcaa at

#cactaagt  93540

ccccaagtag cccccagttc attactatgg gtgatgtttc tgctcccaca ac

#ctatcttt  93600

gctgtaccat tttctcttct tgatagtttt aattatttct agcagctctc tt

#ttctcaca  93660

ctttgtcttg gcttttgagg ttagtgttca cagataagca tgtgttgctt tt

#gtgtttag  93720

agatatggtt tctttttatt tttttaacac cgaataatgt gacttttctc ac

#accctagc  93780

aaacacttta ttagctacct ttaaattttt tcctgtctgt atggatgaaa at

#gatgttca  93840

tcccaatggg gtgttttaat ctatatgttt aaaattttat tgaatattga ca

#aattatac  93900

acgtatatat ttatggggaa cagagtgatg ccatgatata tgtataccat gt

#gcaattat  93960

tgaatcaagt taattaacaa atccatcacc tcaagcactt atcatttatt cc

#tcctatct  94020

aactctattt cttgacttat ggaattgagt aacttttagt aaattatttg ct

#cagtgttt  94080

gaatacccta ggtgactagc actgggccaa aagagaagat gaactcccta ta

#ctttagtc  94140

ctgttataag aactcaattt ttataataat aataataata ataataataa ta

#ataataaa  94200

gaaggaggaa gaggaggagg aggaggaagg ggaggaggag aagaaggaga gg

#gagaaata  94260

aggaggaaaa gactttccat tttatatgca tctttatcag gagccaggca tt

#gtactatg  94320

agctttatat ctaattgaat tcaagtttct ccttagagaa taggcttaaa aa

#cagactta  94380

aaaagttgga tacatatgct gaatttaaat aatgataact tcagtcagaa ga

#taatactt  94440

atgaaaaatt agtgctttag aattatgatt tgccaaatta tagtagtaca at

#ttattatg  94500

taaacagacc aatttaatgt gatttgtcac aggattttaa gtctagtcag aa

#atgacttg  94560

cacctactac aaaaagaaac atgtttatat ttttaagtaa aagaattcca tt

#ttctatta  94620

aaggatttgg agaagtgaca tcattctcta ctgttaatgc tctgtgggtc ca

#tgcataac  94680

agtgaatcag aaagtgtact tgataatcag ggaacatttt gtcctctctt ag

#tgacactt  94740

ttgtaattca tgtgcccaaa ggattacata ttgtttatta atatattata tg

#tcatttcc  94800

tcatttggcc agtgctttga aatggtaatc taatctaaaa aaaatttttt gt

#gtggtcta  94860

tgagaacatt tttttcccac tgagttctaa ggcccagtga ttcattatta cc

#taataaag  94920

aggattcttt attcatcttc atgcctcctt tcccaagcat atccaattag ag

#tcaccatg  94980

tgaaaattca taaatcaaac cgttcgtatt ttaatgtata aaaaaatgta cc

#taaaatac  95040

tttaggtgat acatgctgct ttctcatttt ttaaatttag caggagattc ta

#gcagacca  95100

tgaagtgctg ataactgttt taaattcagt atttattcaa atccaccatg ca

#ggatagcc  95160

acaggaactc ttttatattg gtaacattac ataagtacca atacaggaca aa

#aagatgaa  95220

gcattaatac gtgcctattt tacacattgg taacctattt tgtcacttga ct

#gtaatact  95280

ttgtgcagta aaattataaa ttatgtaact taaaattgat tacaattata at

#tagtagtt  95340

gtgcttaata atttttatat tcttatttgg ttcaatgcct gatcttcaaa ca

#cgaacatt  95400

tttaatcttt ttcaaaatca atatatttgt tcattagtaa atttagcaaa ta

#tttattta  95460

atatttactc tgtgcccagt acatctctca gaccgtggaa tagttttgta ta

#aaacaaaa  95520

acccctgtac tcaagggggc ttacattctg gaggaaagga cagagaataa at

#agtaagaa  95580

taataaataa gtgatttata tgttaaaata agataaagtt atggaggaaa aa

#agtagaac  95640

agagggaaga aggaggggga ggaggaggca gaaaatgcag ggagttgtgt ct

#ttcaattt  95700

taaatagtgt gctgtgttag tctgctcagg ctgccatcac aaaatatcat ag

#atggggtg  95760

tgttaaacaa tagaagttta tttcctcaca gtccagaagg ctagaaatct ag

#gatcaagt  95820

ttcctgccca tttggtatca ggtgagggct ctctttctgg cttacagatg gt

#tgccttct  95880

tgcagtgttc ttacatggcc tttccttggt gcatggatgg agatagagaa aa

#tatggtgg  95940

ggggaggagg aggagaaagg agtgagtggc acacacacac acacacacac ag

#agagagag  96000

aaagagagag agagagatgg agaacaagct ctctgtgtct cttcttataa gt

#cactaatc  96060

ccatcagatc ggggcctcac cctatggcct catttaactg taattccttt ct

#tactccaa  96120

atacagccac actggggatt agggcttcaa catattaatt tgggggaaac ac

#atgtattc  96180

agcccataat atatgatcat tgagaaggta tttcagcaaa cctttaaagg aa

#gtgaagtg  96240

gctacccaga aagatataca aggcatacac acctttgcag gcagaggaag aa

#gctgctgc  96300

aaaagccatg tgtcaacaat ggccttgtgt tattcatcaa taaggaggct aa

#tctggctc  96360

cctaggagtg agcaagcaag gtggggactg gaagggaaat cagaggggta ac

#aggggacc  96420

agacagttaa tgagggacca gatcacacat gccactggaa ggatatgggc tt

#ttctcagt  96480

gggagatgag gaggatttta agcagagaaa taatgtttta aaacgattgt cc

#ttgcttgt  96540

atgttgaaaa taggtggaac aaggacaaag gtggatacag gcagacttgc ta

#agttttta  96600

attcatgcaa gacaggatgg tggcctagat cagattatca gcagcaaagg tg

#gagcaaag  96660

tgaatggaac atacataaaa ctagaaaaaa tggtgtcatg aacccccaaa ta

#cctactaa  96720

ctcaatttaa taataattaa catttggcca catttgtttt atttagacat tg

#ttcactta  96780

tttctgaagt aaagtaagtc acataacgca tattccactc ctaaatactt ta

#gtatgttt  96840

ctctaataag tacattttta ttatattgct gtgattaaac ttaacaataa at

#tattgata  96900

ttatttaagc tacttgtata tacgttttca aatcagtctt tgattttttt ct

#ttaaagtt  96960

aatttatttg aatcaggatc caaataggaa tttacacatt atctttgatt gt

#tctgtttc  97020

ttctattatt tttttaaaga gccttctttt ctcctttcct tcccccctat gt

#catagact  97080

ccctgaagca atcagatagg ttgtcacgta ggaagtccca tattttggat ct

#gtctggtg  97140

gttttctctt gatgtccttt aatttttttt ctttccacca ctttcttata aa

#ttagaaat  97200

aagatctaaa gctttgagta ttcgcagtca acatttttgt cagaagtact tt

#ataggtct  97260

tgctcactag atcaaaatac cttccatcag gaagtgtaga atatctgatt gt

#tacacgtg  97320

atgctaaaat tgatcagtag gcttggtggc agcaacagca tgatccttca tt

#ggaaagtt  97380

ggttttgacc acttataaca agcgtataat ctatacagcg atattttgtc at

#cctgtaaa  97440

tgtccaattc cccattaact ttcctcctaa tcattttaga atacatttat gt

#ttgttacc  97500

tgaatcaact atttcgttag aaatactgat tattaatttt tttttatttt ga

#gctggtgt  97560

tttgctcttg ttacccaggc tggagtgcag tggcacgatg tcggctcact gc

#aacctcca  97620

ccttccgatt tcaagctatt ctcctgcctc agcctaccga gtagctggga tt

#acaggcgc  97680

ctgccaccac gcccagctaa tttttgtatt tttagtagag acggggtttc ac

#catgttgg  97740

ccaggttgtc tcgaactcct aaccttggga tctgcctgcc tcggcttccc aa

#agtgctgg  97800

gattataggc atgagccacc gtgcccagcc ctgtttatta atttctaatt tt

#gtcactcc  97860

tttaacattt atgcagcaaa attatcttct aataatgagc tttatctcct ta

#actagggc  97920

tatttttaaa aagtcataaa atgtagttca gataagaaac cnnnnnnnnn nn

#nnnnnnnn  97980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natttttgtt atttgtattt ct

#tttattaa  98040

ataatcttat tttttatttc tgatcaattt ccatttttat taaatataag gc

#ttgaattc  98100

ttccctcttt ggccagtagg agccccttca ggaggatcat ttgttccttt ta

#taccagta  98160

gccttttgtt agcttcctag atctctagca ccattatatg tcttgaattt gt

#ttagttcc  98220

ttcctcccac tttgcaatta ccatctttcc aaagttacat agtttctttg gt

#gaaaatta  98280

caaatatgtt gaagatagag cctacaggat ttcctcacat gttttggatg ta

#ttctatga  98340

aagagagtca aagatgactc cagggttttg gccttagcaa ctacagtaat gg

#agtgtcca  98400

tcaactgaga ggtgggagga tacagttgaa aggagggaag tgtacatgtt ca

#gttttgga  98460

catatcaatt taacatttta agtatacttc aaaataaaga cgtctggtag gc

#agttagat  98520

atataagtct gaagtctggt ggataaaaac taagtagtca tcatcatata ga

#tgaaatta  98580

aaactgtgag gctagatggg ctcacactgg ggagtgagtt tagatagaaa aa

#gcaagaag  98640

acccgggact gagccccaaa gttaaaaaat ctaggagaag aggaacaaac aa

#gctgctac  98700

attttactag tattcttcag caaagaatat tttcttatgc caagataata tt

#ttttggta  98760

gtttgggatt caaaataaga ttccataata atatttaatg atcctgttat cc

#ctcttctc  98820

caggggaagg actttcagaa agaaccgtag agatcattct ttccgtcact tt

#gtgtatcc  98880

tttcaataat tctccttgga acagctattt ttgcatttgc aaggtaagat tt

#atttgcgc  98940

ttacattcca ggatgcttta tgggcattat atcagtcata gtccaatcag ga

#gacagaag  99000

ccacaacagt tacttgaatg ggaaacattt ttatttttaa tagacagata aa

#aatgtatt  99060

tatcatgtac aatatgatac tttaaagtgt atatgcgttg tgtagtgact aa

#atctagcc  99120

ataaaaggaa agaaatcctg ccatttgaac agaaaacatt taatgtaaag aa

#ttgttaac  99180

tagcagaaat ggctaactac taaagagagt aaaagagaaa tctaagagtc ca

#gaagtagc  99240

aagcaaaaca aagcagctac tctctttaac ttgaaggaga gaggacagta aa

#caactaag  99300

aactgaaaga agttgtctcc caagacttac atggaatcgc tacttctagc ac

#atgcaacc  99360

tatcaccaaa cagtgagcaa agaaatatga cagagggaag gggttggagc tg

#ttccgtag  99420

aagctacccg tcattattag atggtaggca ggctgaaatt ggtaatagaa gc

#atcccatt  99480

cttgctgaat ggtgtaggtg agctggtact acttgagact gttcacgaat gg

#catgggca  99540

gaacgttcac ttcagaggca acaagagctc atccaagtga gctgctgggc tc

#tcacaatg  99600

aataacaata atgcaggatt ggatcccaca agggcagtgt tttcctcttc ct

#actgcctc  99660

tcagggtcac tctagtgccc tctattgaca aagcctcact ttcggccggc tg

#gcaaagga  99720

gaaatgcagg ttccagctcc aatatcaaag agcacagcaa aaaaaaggag gt

#ttggagat  99780

gagagacaac aaggtgaaaa cacaaaagca gaagctttca ggccacctac at

#cttttaaa  99840

gtaatttgta actcttatag gtttaattta aaatatctca atcaggtcta aa

#tattaaag  99900

tttatacaga aagagatctt ttttatagtt agaacaacac ttgtaaaata tc

#cagcttcc  99960

ttatatggta gacccccttc tcatgcttac tttctgaaca tgtctgtgct ga

#attttcca 100020

agtgtatctt tccattctca gcatcagcat cctacttccc ttattattta ca

#gggcctcg 100080

ttggaaatct tacttctgac ctcaaaatct agcttcttaa ggcagattgc cg

#agttaaag 100140

ggaccttaca tttgtaaagt aaactttcta ccaatttcct aaatagttca at

#agactatt 100200

tttatttcaa ctgaagaatg tagttctgta ttctaaatgc catgcattat gg

#ttcatctt 100260

gactctctta aagcataatt ttaatagata atttgaaagg ctcttgaaaa ag

#atattttc 100320

tctataccac ataactattt gcagatttag ccagaagaca gtgagagagt ta

#tcattcga 100380

ggcactttga atgctataat gtgtaaaata tgggcctttc cctaaggagt at

#ggactggc 100440

cacatttatg taatttccct gctctaaaat cttttctgac ttctcatttc tc

#tacaagat 100500

gaactccttg tgtgagtagg aaatggtcct ccttattccc cacaacttgc ct

#actcacta 100560

gctaaagaga tctattctca cctgaacatt ccttgggctt ttatacattc tg

#cttttgtt 100620

cagtcaccct gaaatgtgct tcctcctcct tctcatcctg ggacatccaa gt

#caaattct 100680

acttctttac ctcctctaaa taataataac tatttatgta actaatcagg ca

#ctgtctta 100740

tgtgttgtaa tttgaatctt tttttctctt ttcatgtttt ttctgcattg aa

#atcttgcc 100800

tctcaactaa attgtaatgt ctttgagggt agggaacatg ttttatactt tc

#catatcat 100860

ccttgatgtc caactcttaa taaatactaa atatttgaaa tgtgaaagat ag

#aatagcta 100920

aacattactt tgtaatatac catactgtgt catggagaaa taagcattta aa

#gggtttaa 100980

gatgaaaaga atctgatttg attctcagat tcatgtggct tttatttttg aa

#cctaagtt 101040

ttctgattgt aaagataata tctactcaca atatttttat aaaaattcaa ta

#agataatt 101100

tgaaaataat ttttaagtat tttcatgcat gtaaaaatat ttcatatatg tg

#aacacaat 101160

ggggcattat ctgttagcaa tactattgat agcattgaac tattttcact tt

#ggcatagt 101220

tcctttatat gacaaatcaa tgacatagct agagagaaga gaaacaagat ca

#caacgtaa 101280

gtcttcttgg ctctatattt aaatgtacca atggctcagg ccttcgtcaa ct

#aattcttc 101340

ttaaatttag aacttcatcc caataactta ttagaaaaaa aagaaagtag aa

#taggttct 101400

atggaattaa aacaagaaaa agaagtcgag tagctataaa tttgcaacat at

#tcagagag 101460

gtgattttaa caaggaaatt atttgactaa atgtctttac ttaaaaagaa aa

#ctaaacct 101520

aattttatat actttgtgtg aaactccctt cttggacttt actccgcttg tt

#ttagaatt 101580

cgacagaagc agaaagaagg tggcacatac tctcctcagg atgcagaaat ta

#ttgacact 101640

aaattgaagc tggatcagct catcacagtg gcagacctgg aactgaagga cg

#agagatta 101700

acgcggtgag cacactcctc tgggtgaact gtggtccaga gggcctggag cc

#atgaccct 101760

attctgacct atgcttgttg gaagtgtttg tggggctcta atttacacag gt

#cacagaga 101820

tcttctttca aagagtgacc tccgtcttct acacacttct cactgctgtt ca

#gagaatca 101880

cttaatcttc ctaatatttt gagttaaata tgaactttgg actataatgt tc

#aatcagga 101940

ttattttcct gggacaaata tttttccaca ttaaaccttt gacattatgt tt

#aataattc 102000

atttcatatg atagattttt acattaaact tttctggaag tgtccacatt tt

#caatcaca 102060

ggtttaaatt aattaaattt ataactactt gatattattt atatccattt tt

#ataaaagc 102120

tttttaataa ctatttcagt ataaaagtac ataaaagtct aagttgtata tg

#atatcatt 102180

tttacatttc tttgtattta aaaattaaat ataaagtaaa aagttacctt ca

#gagggaaa 102240

agtaaaaaca tgtgtactaa atatgtttca ttggtaccta ttggaaatag ta

#aagtacat 102300

aattttaaag aaaaaataat tataaatcct tttaaaagca ttatcaatta tt

#caaaatgt 102360

tggcacatta taaaaacttg tctattaaga taattcatca aattcttaat ga

#aaactacc 102420

atcaggctat tttaacgttt gcatttttat aagattcaat aacatgtaat gc

#ttataagc 102480

acaaagtagt tgttaccaag tatttgctca gctctgttaa aattaaaaaa at

#tattatta 102540

attttgaaaa tatggcatca aatgtcttgg actcaaaaag ttattcattt gt

#agttgtca 102600

cttgttaaag ttggtcttta tctaatagat ggactttgca agtatatttc ca

#gcatatct 102660

aaaaatacct aatatgtgct atagagggaa gtgtcatctg ataagcaaag tc

#cttccaaa 102720

tgctacaaaa tgaaggttat tcaatgttat cactaaattg cagggaaatg tg

#ttttcttg 102780

gatatgacag ctgacttttt aaacattcag atgttgatct ttgtgttcta at

#acagtggt 102840

cctatccaca aatggatagt actccaaaga tttaagtgtc agatgattgt aa

#gttatcca 102900

agacatagtt ttctatataa gaaatattat gtacaaaata tcaaatatgt aa

#aaagaatc 102960

aataaaagat tcccagggta actcatctaa gtaaaaccat atcataggaa ca

#caagcact 103020

gctactacta gactgtgtct cagcccttaa ggaatcattc tgcatcatca aa

#gaaagttt 103080

ttcctccttt tcccctatgg gccaaatgaa ttttagtggt atcctcctag cc

#tccttcct 103140

gcactccatc gtcagttcct tttgcccctc ctcaggcctg tgtggcccat cc

#ctttattc 103200

tacaactgaa aatgcacaag ggaaaaaatt caaatctctc aatgcaatta at

#tttagcta 103260

tttgaacaat atagttgaat ctgttcatac taaaatgtaa acttctaaga cc

#gaccccct 103320

ccccaacact ggtaggcatt ttcattttgt taaaagaata cttagtagcc cg

#tgaaaaat 103380

cctgaataag tatatcttca gcaaatgtaa taacgtgaaa aagcactctt tt

#tgtttatt 103440

atgtcatgtt tttaaacagt caatattgga gaaagtatta tttatcgaag ag

#gttacatt 103500

cgaggcagac tgtggtgaga ttcaatcccc taagcactat atattttcac ag

#cttgcccc 103560

tttctctact tctgaacact aaatacatca tcataaaaaa attagaaaag gt

#cgggtgtg 103620

gtgactcatg cctgtaatcc cagcactttg ggaggctgtg gagggtgaat aa

#cctgaggt 103680

caggagtttg aaaccagcct ggctagcatg gtgaaacccc atctctatta aa

#aatttaaa 103740

attagcccag catggtggca tgtgcctgta gtcccagcta ctccagcctg gg

#tgacagag 103800

cgagactcca tgtcaaacaa aaagagtaga tttttttttt ttaagaatga ct

#gtcatggc 103860

agctacagaa aagtttcaga tcatgaaaaa ggtgggcaag gaatgtatag at

#tgtttact 103920

attggttatt tataattcag ggtctacttt atttgacctt cactcttcat ta

#tttatttt 103980

tccacttctg tgtttattta catattgcat tatttgtaaa agggtttaaa ag

#tgaaataa 104040

tatttcagat aatttttatt ttgttacaca cagagaatta gtatatatta cc

#catgataa 104100

tagcaaaatt ggaaatatta gtttccatgc ttttcacttt ttcacttgtt tg

#ttgtgatt 104160

ctggtattca caattgtttg taattccaat ggcacataat aacatgcttt gc

#tggactta 104220

ttacagaaat gcattaaaat aacaattaag tgatttgggc attaattctt ca

#gtacagag 104280

atctgtgtcc agctttacta tttatgcaat atttttatgt taataaagtc ac

#taaaacat 104340

tagacaataa gactggaaaa aataacaaat ataattagct gcatgtacat at

#gcgtggat 104400

cctgtcattt ggtgaagctc taaaactctt catctgtttt gaggtgtttg aa

#gatctaaa 104460

tctgttcaaa gtcaatcaga gactgatggt agattctagg agtgagaatc aa

#gaagtctg 104520

atttagctcc ctaaattgtt ggcagacttc caccatatgt ctttgttatc tg

#caggaaag 104580

aacttccata atttctctta aatctaccca gctaataggc tgggcatagt gg

#ctcatgcc 104640

tgtaatctca gcactttggg aggcctaggt gggtggatca cctgaggtca gg

#attttgag 104700

accagccggc ccaacatggt gaaaccccat ctctactaaa aatacaaaaa tt

#agccaggc 104760

atggtggcgc atgcctgtaa tcccagctac tcaggaggct gaggcaggag aa

#tcacttga 104820

acccaggagg ctgaaagtgg cagtgagcca agatcacacc attgcactcc ag

#cctgggca 104880

acaagagtga aactccgtta aaaaaaaaaa aaaaaaatct acccagctaa ca

#tacgctct 104940

ctctacttga ttcttaggca tgtccttttt attccaacct gctaaatttt tc

#atgcaaaa 105000

ttgagctcat aacttttctg agtcctgtat gttttcccat gtccaaaaga ta

#aatgataa 105060

aagaagaatc tctaatcaat aattaaaata ttaattttag gaagttacca ac

#taggcaaa 105120

aataaaacaa aaaacaaaca tggtgcttgg tactaagtcc tttcaatgat tt

#gtggtttc 105180

atattttaga aattatttaa ctattttata ctctctgcct gtatatttac ac

#tttaaaac 105240

ccattctgta atttttgtta tttgtaaact cattatttaa ttatgctcca ct

#ttgtttca 105300

caaaactttt gaaaatgcct tctctcactc tatcattcta tactatttct tc

#ccaaatga 105360

gagcaggagt caaataaaga tgtagtactc tttaattcta tgaaaacatt ca

#aggatata 105420

ctaaaataac gttttaaatt ctcaatttga atgataatta tattatgtac aa

#agattatt 105480

cacattttat gtttaagttt agataacaca aactataatt cttaggaaga at

#atgtaaca 105540

ttttgggctc atctgtttca cacttaccga attaggaaat gatccttggg tt

#ttgttatc 105600

taataaacat acagaacaac attttgtgat ggctcctgca aaacaccacc ac

#ttagccca 105660

ctgaagttag aaaggtttct tagagctctt attggcaaga tcagcagaca ca

#gacacgca 105720

cagtaagaca cagacctgta tcactgagac tgactcacct tgtggattgc ct

#ttaactac 105780

tttaactgta caacgattac cttcccatga gagtcacatc acttataatt aa

#ataaccca 105840

acagaatttt cgtaagctaa aaatgctatt tgctaaataa gcttattttt ta

#ctatcttc 105900

tttccgcatt taagtcacgg aagttttgtt tcttatgccg actaaatcag aa

#aagaatag 105960

taaaacaaca ttaatcaatg tcactaatat tcttacttga cagaaactca gt

#ttctttta 106020

gtcctcaatt ttttttcaaa aattttatgc accacttcat attaattacc ct

#gcctattt 106080

tagttgagtg aatgtaatgg cacattattt taagcctcaa agcccaatcc aa

#taatcacg 106140

aatagaatta aaattcacaa gataaagtaa acaatctaat gagttggaaa aa

#tttctatt 106200

ttaagagaag tcttcttcaa taattttctt tcttcagtaa cttcagaagt gt

#acatgttg 106260

aatttttgtt aaatacacag ttatgtcttc aggaagatta ctgcttaaaa aa

#attctaca 106320

tatgtacttt gtaaactgta aaccagaata cctttggtat tgttactatt gt

#gatttata 106380

tttgtaatat tgaatactac cccggtctac tttcatatat agagtttgct aa

#caaaataa 106440

tagctactgt ttatgagcaa ctcctgtgtt aaactctgca tgtagtgatt tc

#acttaact 106500

ctttcaaacc ttagtggtag gtacacctat ccccatttta cagatggatt aa

#aaaatgat 106560

gataggacag gtttatgtaa atgtctaagg tcatacagct aataagcagg ag

#agctacaa 106620

gctagcccag gtctttctct gaggtatgaa gatacacgtg tgtgtgtgtg tg

#tgtgtgtg 106680

tgtgtgtgtg tgtgtgtgtg tttaacttcg aagcatacta gagaagatta at

#gtaaactt 106740

ttcttaaata gacaaagacc catggaattc ttcctcagca gcattttcta tg

#atgagaat 106800

gagtctaaag aaagaggttc ctctttggat ttctttgttg tctcatgata tg

#aagcaaag 106860

aagtgatggc atttaatgtt gactcaaact tgcaggaact ggaaaacttt ta

#gtagtgta 106920

atttttattt tggcttcaat aataactcat aatttttgac tgatatatga aa

#ttctaata 106980

gttcacattt taagtgtcat cattctttga caaattctgt tcatatattt ta

#cttccgtt 107040

ctaaacttaa gctatctttg caaacaatgg caaaaatttg tgaattcgga at

#acaagaaa 107100

tgttctatgc ttagaatgaa attggagata cttaatgctc atattcttgt aa

#taacaaat 107160

caaaaataat tcagtgtgtt tgtatactaa ataatgaatc tttacttgca ga

#tactcttc 107220

atttttcttt agacgcaagg agatttttgt catccagtaa gttactgtgg ta

#atgcagaa 107280

ctctgctgtg attattttaa tcttgtcagg tggtgtgctc tatattttaa aa

#tacataat 107340

attgaacatc ttgttgttta atgcactatt ttttccaaag ctccccccaa aa

#gctatatt 107400

tctatttaca acatgtcctt tataatattg catgctattg ataatggtca ag

#ttaatctt 107460

atcaaaatgc acattgactc ataatgtgca tgtcctgaga atttgctgtg tt

#ctcatgtt 107520

gtgttagatt tgatagcaaa ttaagtttgc acctagattc ctgtacaggc tt

#ctcattct 107580

gttatcaaca tgacgcaaga gttgagctct acatctgatg ggtggaaact at

#atttacat 107640

ttcatacaag ctcatttttg caactgtaga tggttaacct gtaaggacca ag

#acaatgac 107700

atttcttgtt ccctgactct ctagtgcata cacagaatgg catatttcat gg

#aaacgtta 107760

tttctccact gaccaatggg tagccaactg tgcacgcttc caggcactcc cc

#tgatgctc 107820

agaaatgcca tttgtatcct ggcacaaaca ttttttgtta cattctgaga gt

#agcatagc 107880

agaatatcag cactagcagg gaccccagta actgattgag cgtcccaaac at

#aataaatt 107940

tcttcatgca aagaatgtaa atgaaggaat atgaatggag gcagagaata aa

#aaggcatt 108000

tgatttcaaa atcacacgcc ttactaaaga agaatccgtc ttcatgagct at

#aaggctga 108060

atggggccaa agctcctgat agtctggtta accatgaata atactctgca tt

#attaaaat 108120

caaggaagcc cggtctattt ctaatctaat cacatttagc atttgggaat ca

#taagtaac 108180

cttgttttaa cttcagatta actagttacc aagttcccat tgacagaatt aa

#aatacttt 108240

aatgaaaata catttccttc agaggacctg cttgatgggg ttcaaacatt tg

#tcaaagta 108300

agacactgtt aaactgaaga tttaattgat cacattacac ataaaatatc aa

#ttttcaac 108360

cagcactcaa agttaacctc tgggccattc cagactcaga ggcggtttgg tt

#gagcaact 108420

ctgctgaatg tctttcttca tcatcataaa atagaatcct tttcctattc tt

#tttctcct 108480

tctctctttc tctctctctc actctctctc tctctctctt gctctctctc tc

#tcgctctc 108540

tctctttctc tccctccttc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 108600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 108660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 108720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 108780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 108840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntattcatt gagtcccttg ct

#cagggccc 108900

catccatctc ttcacataac tttgatcagt ctctttctcc tctctatcta ca

#aattctac 108960

cctgttgccc cggatgtagc taaacaatgc ccatggtttt catttagaat ac

#atggttga 109020

caaaagaaga cttctggcaa gaatatttct tcaaatgtgc taatgtggaa ag

#gcttagta 109080

ataaggaaaa ttcaacttct gccacactgg ggatcatacc tctgagcttt tt

#gacatcag 109140

caagaatatt gcattcactt ctcatctaaa aggccatttc atcttgttta aa

#taaaaata 109200

aataacaatt gagggccggg cgcggtggct cacgcctgta atcccagcac tt

#tgggaggc 109260

cgaggcgggc ggatcacgag gtcaggagat cgagaccatc ccggctaaaa cg

#gtgaaacc 109320

ccgtctctac taaaaataca aaaaattagc cgggcgtagt ggcgggcgcc tg

#tagtccca 109380

gctactcggg aggctgaggc aggagaatgg cgtgaacccg ggaggcggag ct

#tgcagtga 109440

gccgagatcc cgccactgca ctccagcctg ggcgacagag cgagactccg tc

#ttaaaaaa 109500

aaaaaaaaaa aaaaaaaaaa ccaattgagt atctctcaag tgctaggcac tg

#ttttaggc 109560

actgggaata gtgtgatgag aaaggaagaa acattgcctc caaagagcta tc

#cttcaaat 109620

ttaatgcttc ttttaaactg atttgtccta cacatatgag aagatatgtt ga

#gaaggtaa 109680

tacatatcat ttactattaa ttgttttcct gatttaaaaa agtattacat gg

#ccaggcgc 109740

ggtggctcat gcctgtaatc ccagcacttt gggaggtcga ggcgggtaga tc

#ccctgagg 109800

tcaggagttc gagaccagcc tggccaacat ggcaaaatcc cgtctctact aa

#aagtacaa 109860

aaattagcca ggcatagtgg cagacacctg taatcccagc tactcaggag gc

#tgaggcag 109920

gagaatcgct tgaacccagg aggcagaggt tgcaatgagc cgagtttgca cc

#attgcact 109980

ccagcctggg caacaatcgt gaaatcccat ctcaaaaaaa aaaaaaagga ag

#tattctat 110040

tactcactta tggtatattt ctcttataaa attttagaat cagctgtata gg

#accaacca 110100

tgggttagga tattttaaat ttatattgag caccacggaa acatcaatga tt

#tggtaaaa 110160

gaatacagaa ggatgtgaca gaatgcagaa ggatgtgaaa gaatgcagaa gg

#atgtgaaa 110220

gaacaaaata aagaaaacta ataggaaata acaaaaatta aggcaccttt aa

#aagtatta 110280

aaatagatgc tttggataag cgatagaata ttgtagaagt agatgtaatt ta

#tgtgtcat 110340

tagtgacttg atgaaatata taaactaaaa actcacactc agtatcatac aa

#aacttgga 110400

aatattaata ttgtaccaga gaaatagatt cttcacaaat ttaatctaag ta

#gcaagtac 110460

atgctatggg atacaaatac atatttttac accaattgac aaatttgaga tt

#cttttatt 110520

tttaacttaa catcactggt taagtagaag aaaagtttct cagtttgtcc ca

#taccactg 110580

gtaatgctgg ttgaagctgc tcagctatag gttatcatct gtggctctct at

#taggacta 110640

tattttaatt ccctatagat ttcaactaat tgaccttgag ggaaagctga gt

#ctctgtga 110700

caatatggtc ttcaatcacc actgccaaca taaataaatg cttcctttct ag

#atccatca 110760

agagtaatct gagtggaatt taagtttttg ataggcttta aagaaatgag tc

#cagacgtg 110820

aaataagaca cttttcaacc aaaaggatat agaatttaag acagttaaga tt

#cgtgtaat 110880

aaaaagtgtt tagccctttc tattggaaat tagtcagtta tcttattgaa at

#ctggacag 110940

ttcccaaatt gatttatcca gtaatgaact gattatagtc tgacagtaac ct

#tcactatc 111000

ataaatgaat atcctaccag tctaaaaatg ctttccattt aacagttttt tt

#ttaagttt 111060

ttaaaatgtt aataaaaagt tttctatttg agtatgtttg agtatctcct tg

#gatcactt 111120

cattcgaaac tagcactcct gaaatagcat tgttgatttt catgcacatc aa

#tttctgtg 111180

agtttctagt gcttatttaa gcaaacagtt tttcctatta ggaatttaat ta

#tacctctc 111240

agtgataagt tagtgcattt tccttatagt atgtcccatt ttcttttcta at

#tctctcta 111300

taaatcagtc aaattaattt ttttgtatat aaacattaaa gcttaaaacc tc

#aaagaaaa 111360

atacaattta gaatgtagcc aacacctaag ggagaaatac acctatacaa ca

#tgaggcta 111420

agaacgaaag caatgataag tatactacag acaacaatga ggaaggaaat at

#ctaacttt 111480

tatttgaaat agtcaggtaa tgtacctcaa aatgtcttct caatttgagc at

#tcctaata 111540

ggtatttgaa gatttcaact cacaaatgat tgtgacataa gtacagacta ga

#aaattaca 111600

taaaaactgg actactagaa gctttcttat cttatataaa cataaatgtg aa

#gaacagat 111660

tctaaaaagt gattggattt agataaaaaa gagtgataca aaagaaaata aa

#gccaaatc 111720

agattccacc tctctttttc ttaaagtgtg tgcctatttg tttatcactt ga

#gtaggcaa 111780

gagcaatttt attgttcatt tatctaactt cctaacaaag tacacctgtt aa

#tttataac 111840

gttaggttat ctgctatggc ttttgcttag actcacatgc tttttgttga ta

#aatctatt 111900

gattatacgt atttaaagct ttgagttagg acctcttgag aattctcagt tt

#cttaataa 111960

tttagtgtga aaatgtattc aattcagata ttccctcaca ataaagccag aa

#tattcata 112020

ttttgctttc tgtgtatctt aatctgaatt catccacaat tttatatttg at

#atgtttta 112080

tttaatgttt actgtgaata atgttatgag ggacatctag taagccaagt gt

#taatcctg 112140

ccccagccct gaagtatata tgagcccaaa cacttgtatc cttaatgcag gg

#acttaaat 112200

agccataata caacatagaa gatgatttgt ccttggaaat ttgattttac ag

#gcaaagga 112260

aattattttc tttttagtag aacagagtaa gatcgatagg gttgttaaca tt

#tgaatcag 112320

gtattaaaga ataagtaaaa tttccgttgt acgaagaatg cctggaatgg ta

#taaaattg 112380

agagggaggg atatatagag aatatctgga gtgcaaacag gatgcatgaa ga

#ggagttac 112440

aaggaataat gtcagaaatg tgggcatggt tagaaatgtt ttacatgatt at

#atgaaaac 112500

tgaattatta tggtcattgt attagagatt tgtttgggat ctcgaattga ga

#gctagaaa 112560

tccagacttg gatttgaaag ctagatattc gtgactacta tattttagca ca

#atatagtc 112620

tatccatctt tgagtaaaat taagagaata ttcttttgga aataatggaa aa

#aatccctt 112680

ccttatatca gtatcaattg tagaacagta tggataggag cagcttgaga tg

#aacaaact 112740

aaattagcaa tagtaattat catactattg atagtaacca atacttatgt at

#tgcttact 112800

aaaggcagag acctttaaat atatgaactt aatttaatac atttcccaaa ct

#aggaaact 112860

gaggcacaga aaaattaagt attgcacatg ataatataac tagtaattgt tc

#aagcaggt 112920

atttgaaacc aataaaggca tcatattttc taataaggca ataattcaca aa

#tatccacc 112980

caaacccatt atagccagtt atggtttaaa atatctttag gcggacatca tg

#aaatgcac 113040

atctttatta tcccccttga ggggtgaggg agctggggta tttatccacc aa

#ctctggtt 113100

agtcattggt tgatggatgt ttcttggaat atttaccctc cgatgcttct ag

#cctggatg 113160

caggagacac tcgaggagag tggcaggtcc ttgtagtagg aaactatctc ct

#tgcatgcg 113220

aatgttgagt gcccaggcga tgtgggttag gcaccaatga catctgcaca aa

#cttttaaa 113280

aatctgaatt tcacagcact taataaattt acgatgatgt atttctgcga aa

#aaaaaaat 113340

ctttagggag agattttaaa tgcaaaatga attaagaata gtgaaacagc aa

#cttttggt 113400

agagtttttc actgaaaaga catgaactta aaacaaaaaa atgtatattt at

#tcaattaa 113460

tcataacttc tgaaatgaag aatagagatg attaaagaag agcaataata tg

#aataatat 113520

tttgctttag ctatttcttg ctcacttttc tttaatatga ttattcacat tt

#aatgtctc 113580

ttagggattt cacaaatgta tactgatgct tcaaatggtt tattgacatt tt

#cccagaaa 113640

ccaacatcta ctttagattc tagttatctc agtaaaaata cttttgcagt ac

#cggctcaa 113700

atgatcctct aggaaaaagg aatctctctg cgatgggtgg cagtctcact gt

#ccttatat 113760

aggtggacta ctagcctgtc actaaatcat atatattgtg cttaaatttt gc

#caatcaca 113820

atggaataat atttgctgtt attataaaag ttatttccac aaagttcaag ag

#tttctatg 113880

tccatgtggt agcagggaaa tagaccttgg taatcaaagc atctcagtca tt

#tatatctt 113940

aagttcagtt gatcagaatt tacccaacac aaccttctat tctttcctat tt

#ctgaagaa 114000

caaggtatca taggggcact gggcaacaag ttatcttaag ggagctaggt ag

#tatgtgtg 114060

gatgtagcct gtagtttatc tttctttctt gctggtcttc gctgaggggt aa

#ttattttt 114120

aacaaagatt gattgtggct tcagtcccca ctgcaactgt tactatgtca ga

#gatatttc 114180

cagggcctcc aatattcaga cattctattt tcccttcccc aaatcaaaga tt

#cttctcat 114240

ttggtagccc tttcagccat ctccatatcc atctagaata aggaattctt tc

#ttgctttc 114300

tttaaatcac tctagggtat tgtggggcac tcttaagctt atccaccaag ac

#tctttgtt 114360

agtcactgct actttgtcac ttagatgccc tgtttggcaa tggaatagtc ta

#tcacttta 114420

tgtttaccct gagaagctgg aagatacaac atctctttct gcttgggggg ca

#cccatcat 114480

taactgagaa ttctaacatt ctactttgta atacctggtc cagcatcccc at

#atttttca 114540

acaattcctg tattgtaatg aaatatactt ccttttaaat cctgttttct tc

#attgaata 114600

cacctctttt tgaccatttt catatttatt atgctctgtt tttcaaacca tt

#ttttttct 114660

tttattcatt ctttgcttca aaaaacatat cttcttacaa atattcttca at

#taaagaat 114720

atagtaaaat ccctaatatt attctagatt taaaactttg aaaaagtcat at

#gttcctta 114780

gttcatttca ttatattttg tgccttttgt gttttttgca gtgctaattt gt

#tgtgcatg 114840

acgtaagtgt tattaatgat acgcccctct ctaagtttgt gtatgttgtg ta

#gcctattt 114900

agctgttaaa attatttttg tttacagagt ataagtaatt tggccaatga tc

#tgtcacaa 114960

aagataggtc taaaataatg gaaatagtta taatttgttg ttgctgtgta tt

#tatccaaa 115020

ctcactcatg aaacaatact taaccaatgt gatgtcatgt ttcatggatt ca

#ttctgtct 115080

ggttcaacac tttctatata tagaggaaat atttttaaaa tccacattag ct

#cttttaga 115140

ccactaaata ccatgcaata tattaaaaag tgatctattt ttaatgtagt at

#cctaaatg 115200

cctaacattt ttaagcattt ataatgacat ttataataac aacaacatct tt

#tcagctcg 115260

agaaagaatg taaattattt gccatgtttg agtccaaata atgtaatttc aa

#aaaaataa 115320

ataaaattta aaataatgat catatattag ttaaaggcat agcacatttt ac

#attattga 115380

tttattataa ttttctgact ttaatctaca cttctttcag aattagctgt cc

#actctgac 115440

tcacaatgca tttaacacaa tctctattgc aggttactta gttatagaaa at

#ccatcaag 115500

taagtttgtt aaatattttc tttcttcttt ttgaatatca aagttagatg ca

#ctgactca 115560

gtagaacctt aatgtgtgat tcactttttg tatgtttgtt ggaaaaacct cc

#aagctgga 115620

tataaatcat aaaagcatga ctaattgcat ggtaactgga gaaatgcttt ct

#ctctctct 115680

ggggtgaagc ctgcatgtct gtattttagc ttgggaagta atacggggat at

#ttaaactc 115740

cttggggttt gaaaaccatg tcattatgag aatgaggtca ctgcaatatt tt

#atatcttc 115800

taaaaccttg taatgtataa aatgttttct gtctgacaaa gaggtattat gt

#gctttagg 115860

agtcaatgat aacttcatgc ccttacattt acttgaaaaa ttttcttcat ta

#aaatgcta 115920

aatcctttat ttaatgtcac taaaaaattg aaggaatttt gtgccatgaa ta

#caaagaaa 115980

gtgagcttaa agaagaaaag ttaattttat aagtataaca gagtgacttt aa

#aaagctgt 116040

gttgtttgtg attttgggga tgtccattgt tctttaactt gttaaaagtg aa

#gccagtgc 116100

caatgctaac gcgaacaaat acaatctaac atgaccctat tttatacctt tc

#tttacttg 116160

gaggccaata agcaagaaat ccttcctgca acatgttgaa gagctttgca ca

#aacaacaa 116220

cctaaagttt caagaagaat tttcggtatg ttactagcag ttgtcacaac at

#tgcaagac 116280

ctccagtcgt ttcatgtgtc acatttcatg tccattttaa gcaagcaagg cc

#atgaagga 116340

ctctggcctt gataatcaat acccaattac caggttgatt gttttgatag ta

#atgttaca 116400

ctgggccgcc tctggtgcaa cctgatcaga attattcacc tactgtgtca gg

#aaaaggtg 116460

gtcttcttca gacctcccct gtattggcag catgaccttg tctattctct gc

#tctttcat 116520

ccagatgtag gtgcaaatgt agaatgccat attcattagt ttgttttgta tt

#caaggttt 116580

aggtcatact ataagtgtag ttttatattt aagtaattat tttacatttg ga

#cactaaat 116640

tatttcattt tacgtttacc tacttggttt acattagtga tatagatgaa tg

#tgagatca 116700

aaacttgaag cttccagaaa ctataagaaa attatttcta gaactgtcta aa

#aataaaaa 116760

aaaagataag taatgtccac gttttacagg gggccttttt aaagttacta tg

#gaataaat 116820

gctgtatcat ataatgaaaa tgtataaaat taagaatttg tcactttaaa tc

#tacttaaa 116880

agttgggaat agtttttttt ttaacatttt gtatatctat aaaattgaaa tt

#atttaaaa 116940

acataaggta gatatcaaat cttcaagcta ctttaagagt tataagcatc tt

#ttctaact 117000

tagatgatta ttttgttatt aagaaagaca gatttctaca tgtcaccaaa aa

#cattattt 117060

ctattttatt tttttccatg aaatttccag tgtgtgaact cctgaaacaa ag

#aataaaac 117120

aattgggtta aataattcag aattataata tttcagtctc ttagggaata at

#aacaaaaa 117180

tgagagaaga ttaatggtat ttcctgcagc cttttggtta tgcttcttaa ga

#aatattgg 117240

tctggactta acaaaatcaa tagtgccata aaattcttcc tagcatttag ac

#agcaagaa 117300

ttctcaattt ttcaggagca aaagtgtaat ttccctagaa taagagtgaa tg

#taattaca 117360

ttatgcatga ccaagtagat aaaaagtttt attagcaata acattttcac at

#atatgaga 117420

aagtttctag tttaagtttt ttgaagacca tagtttgaag aactttttaa aa

#atttcatt 117480

ttgtctaatg ctttgttaag aatttctaaa gcaaattatt aaattatgtt tt

#aataaata 117540

catttttggt gcatatattt gataaacctt ttaactcagg acatattcac tc

#atatctta 117600

aatatttata agttcctact atgtgatagg cattgtattt ggcacataca at

#cagcactt 117660

accagctaag tgactttggg caaatttctt aaactctctt tgcctcaata tc

#ttcatttg 117720

taaaattata atatctactt tattaaatta tgaatataaa atgattgaat at

#aagcaata 117780

ctaagaacag tagctggcac aagtattagc tattacgatg ataaattcta cc

#ataaagaa 117840

gctcatatta tggtaagaac tgcagatgtg taaaaattgc aaatttactg ta

#tcttgatg 117900

gagatatgca tgaagattga ggatcaaatc tgtttgtata tcaaccagaa aa

#gagtttat 117960

aaaaaaggtg tctttcagga tgaactttag gatgagcaga agtctaatga ga

#aggtaaag 118020

gaaaaaaggt gttccagaga caggaaaagg ataggaatgc aaaaacggtc ta

#actcatct 118080

gtggtcccac tgaaaagaga gaaagcaata ggagaaagag ctaaataggg ag

#aattgtgg 118140

ccttgtgcac cctgataagg ataacactgc ccagtgtaac attaccatct aa

#acaatcaa 118200

cctagtgttt gagcattgct taccaaagcc agagctgctt aagtctagaa at

#ggaaactt 118260

ttatgtgaaa taattataaa atataagtgc tctttcagtc tatttaaact ca

#cctttttt 118320

ctcccttctt tatgtatttc caaaactttg acacaaggaa gctgttctag ga

#ctccttat 118380

tgggttaaaa aaattttgta agcctttggt gccaacacat cagaattcag at

#cttacacc 118440

attcctgtcc cttaatacat acccatatgt aaacaattgt ccagatttta at

#tttgagaa 118500

aaaaaaataa gaaagggaaa cttatccaat taaaagaaat aatttatatt ga

#tgttgaaa 118560

aattgttaaa acacatattt ttagtgcttt ttatggaatt tggcaagttg ag

#atttctaa 118620

aatggaaaac tataaatttc acacatgtaa attttcagct caacaaaata at

#gaacatat 118680

tttcttatgc cagacttttt aatgatgctt gctttgccaa gagcaaggta ga

#tagataat 118740

atcataaatt ttcatcaaat gtcaataata gatcttcttg acccttcatc ta

#tatctgat 118800

aattcttaat tgcacctttg cattccatta ttgatttagc aatgcttatt aa

#gagcagga 118860

attgacctct ggcatcttct caaactgacc aatgttgtac caattaatag gc

#atgaaatc 118920

acactgcctg aggagagaaa acaaaaaata aatcattgaa atcccttttc ct

#actaagta 118980

gacattaaaa tattaaacaa tagtgtgtac ctgatgaaac cattagtaac at

#atgtaaaa 119040

tggtcactaa catggttgcc acatcttaag gcttcccaaa gtgcaaagaa at

#tatctccg 119100

aaagagcaaa acccagctga ccaactttat tcaggatatt tttggtgaaa gc

#ctaggtaa 119160

actcattact gttaacttga ccttgtttcc acagttatga taggtgtttt ta

#atttaaaa 119220

attaaaaaaa aagtttagga caaataactg tcttttaata agtgaaattt ct

#tgtttacc 119280

tctgataaat gtaaactttg taatgacttt attttacagg aattaccaaa at

#ttcttcag 119340

gatctttctt caactgatgc tgatctgcct tggaatagag caaaaaaccg ct

#tcccaaac 119400

ataaaaccat gtatgtgcat ttgttggttt tggtttagct aggaaatatt tt

#taaatgcc 119460

taccatctta acttttttgt ttccttaata tattttattt tatattgttt ga

#attataat 119520

aatgtatttt attggcagtg actaccaaat tatatatctt ttgctttgtt ca

#tatttaac 119580

taaagttagt atacgtggtt ttcagtttgt tcacacaagt tcacttatgc ag

#gtgcaaga 119640

aactgtagac ctaatagttt cttagctttg taattaaacc caagtaatga ac

#ctgtttaa 119700

catcttccta cagacctagc atcaaatgca aagggaatat tctcacttag ct

#ttgtgcat 119760

tagtttccct tcacagcata gcagtgtttt cctatggaac ataaaaaaaa tg

#cattgtaa 119820

aatattcatt gaagaccaga gtaaatgcgc acttacatga attcatttta ca

#tatgaggc 119880

agaatgagct tcaccatagt acatacagct tcatttttca atcaataaga aa

#ataaacag 119940

tgttattgct taaagaatta acagtgatgt gaaaggaaaa taggacattt ct

#ctgttact 120000

aataactata tgtttgctat tatatttttg aacagatatc cctattttca at

#attctgat 120060

tcaatacatt ttacactatg aaattaaaaa gtgacattgt gatgtcctga aa

#cgtttcaa 120120

agtctagagt tttgaaatgt tcccaatttt aaagataata tacatctgtg tg

#tgtatata 120180

tatatttttg tctatgcttt ttcaaatgta tattggggga atagctacac tt

#cttaaaaa 120240

ttgaattctt ctttctagaa agacgtagac ctagagaagg atgtgatcat ag

#aatgtcga 120300

attgtaaagg acatgcatag gaagaaacag accaaattct gatattctag aa

#ctagggaa 120360

taacttatga agttcacgaa ggaaacaaat gtaaggaaac atgatatagg aa

#gtagtaat 120420

ctactagaac ctgttaattt ctaagaagta gaataggatg aaaatacaaa ta

#gctttaac 120480

cacctaaatc ctttctgcaa caaaacaaat cataagtaaa tgattttaag ag

#caagataa 120540

caagactcag tcagaggaaa taaacattca taatttcttt ttctttcttt ct

#ttttttct 120600

ttctttcttt tttttctttc tttcttttct ttctttctct ctttcttatc tc

#tcttcctt 120660

tcttttcttt tctcttttct tttctttctt tttggacttt tgatcttgtt gt

#ccaggctg 120720

gagtgcggta gtgcaacctt ggctcactgc aacttctgcc tcctgggttc aa

#gcaatctc 120780

ctgcctcagc ctcccaagta gctgggatta caggcatgtg ccgccatgcc ca

#gctaattt 120840

tgtattttaa gtggagatgg tttttcacca tgtttgtcag gctggttaaa tt

#tctaagac 120900

caggacattt ttcaacgcat ccttttattc tttcttttag agagttaacc ta

#ggttggat 120960

gcaatgtaag tataaaagtt atggacattc ccatgtcttt atatttatct gc

#acctaaat 121020

ttcatccaga ctgtttgctt catgcttatt tggtgatatt ctgtgaaaat aa

#atactgta 121080

atctgattaa gggacagtaa ctgtggaata attagtattt tttcaattgt at

#agaattaa 121140

tggcctggcg cggtggctca cgcctgtaat cccagcacgt tgggaggctg ag

#gcgggcag 121200

atcacttgag gtcagaagtt caagactagc ctggcaaatg tggtgaaacc cc

#atctgtac 121260

taaaaatcca aaaaaaaaaa aaaaaattag ccgggcgtga tggcaggtgc ct

#gtaatccc 121320

agctactcga gaggctgagg caggagactt gcttgaacct gggaggcaga gg

#ttgcagtg 121380

agcccgagat cgcaccattg cattccatcc tgggtgacag agcgagactc ta

#tctcaaaa 121440

aataataata ataattaatt tataatatat cagtatatat aaagtaagta at

#tcaaatat 121500

gtctaactta cttccaaggg agtgtctaat ttgaaatatg taaaatttga ta

#gatcagaa 121560

agtttttttg ctgtgtgact tttnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 121620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 121680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 121740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 121800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 121860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 121920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 121980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 122040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 122100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 122160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 122220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 122280

nnnncagaga ttgatttcat tttaatagaa tttgaatttt agctaataga at

#ttgaattt 122340

tagcttgagc cagctaaatg aagtccattg tttctctccc cctgagtaat gt

#aagaagca 122400

gaatgccaaa tatcacagca tgttttagaa gacattagct taaaacactt ta

#agatgcta 122460

gaattagcag caaatacttg cagaagagat ttccaaatct ccgtttgaca ga

#cgacctat 122520

gaccttcaga aatattccaa caccacaaag aaaagtttag cttgtagttg ac

#tcagatag 122580

ttataaggag gtgtatctag tgaatagaaa cataatgatt ctcttcctta gt

#aacaggac 122640

tagttacaat gtcattaaac tagatcatat aaaattctat atttgggaca ta

#aatcattt 122700

gcagaaatgt gtttaatcat tcccatttct aaacataacc acattgggca ag

#taattgtg 122760

attactaaca atacaactaa ttcattttaa catattatgt gcttaagttt ta

#agaatgta 122820

agaacatgta ttgagtgcct attataagct ttcatgtaaa ttttcttcaa ga

#ggattata 122880

caaaatctgt gttataatga catcttgtag ctgaggtaac caaggagcag ag

#aagcttag 122940

caatttaccc aagcccatat gtatggtagg cagatcatct agaattcaga cc

#caatcctg 123000

tttacccatt tttcctcctt gaaaacaaac aacccatcaa aacaattaag ta

#gtggatat 123060

ttcatgttta gttaaaaatc attactggat gtttcaatta taatatcaac tt

#ggtaaaat 123120

ttctcaggaa aaatagtttc tgacattttt ctctgaagaa aagtaatgag ta

#cagggtat 123180

ctagttttac tagctcttaa aatattctaa aaagtagtaa ttagaaattt ga

#aagaaaga 123240

ataggcagat gaattaatgg aaaagaatga atggtctaga aatagaccta ac

#tacctaga 123300

aaacaaagtg gtgttccaaa tcagggacaa aagatggaaa tgttaaaaaa tt

#gatactat 123360

ttgttgttcc aacgacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 123420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 123480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 123540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 123600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 123660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 123720

nnnnnnnnnc agtttcactt gtatttaagg aaatcaaaat aaatattatg ag

#acatcaat 123780

ttgtatttgt acaaagaaca taaagtgttt aataatacct tgtgataatg aa

#agtaagag 123840

aaaacagaat ttttcaaaca ctttggttca gagtatgaat tgttagagaa ca

#attgagaa 123900

atgtttttca aaatttggaa tgcatatata taaaatctaa aaattttact gc

#tggtagta 123960

tattctacaa gtacactcct atatatatgc aatatgcaaa aatttcaatt at

#atctattc 124020

aacattttat aattacaaag tgaatcattt aaataataat aaaaggttaa at

#aactacat 124080

tattatatat tcaaacagtg aaatactgta tagcttttaa aaataattaa ct

#acatatct 124140

ttgtttatgc tgatacggaa agacataggt aaatgaaaaa aacgatttgc ag

#aacagatt 124200

gtatatcact ccatttgtag ctaaagaaaa ttagaatata aagaatatgt at

#agaaaatt 124260

ctggaaatat atacaaggac atattaatag tcattgtctc tgggtggtag aa

#atatggga 124320

gaagaaataa ctttttaaaa tttaccgcca tctgtgaaat tggaatgtta ta

#ttatgaac 124380

tcctttgtat tacttttata gtaaaaaaag tagtaacaat ttaaaaagcc aa

#ttaacatt 124440

gattccttat attttcttct agataataat aacagagtaa agctgatagc tg

#acgctagt 124500

gttccaggtt cggattatat taatgccagc tatatttctg taagttacta tt

#ttatatat 124560

tttataattg tataaaacat aattactgaa attgtattat ctttccaatt ac

#ttaaaaca 124620

acaaatttat tacaactcct atggatctta atatgctagt tatttacagc ca

#cattgtgt 124680

acccttattt tatagatgtg gatatggata tgcctaacag agatactaac tt

#atcaaaaa 124740

ttatttcacc agtgcgcggc agatgttcaa cttcaggcta cacatccctg at

#ctttccac 124800

taattcatat gctttgttaa tgtattctcc atatgcaatg aagtttgcca at

#ctctgtga 124860

attaaaaatt atcaaatgga cagttatgtc catataacat gaaaatttat ta

#tgcagctc 124920

ttcccttcta gatctgcagt ccttcaagcg ggtaataatg ccatcaccat ca

#taggtaca 124980

ttgaaacctt atatgcactc aagatctcca cttggtttgc aaattcatgg aa

#tcttaaag 125040

aaggaagtgc cttgaatttg accattcacc ttgaaactct aaaaaattcc tg

#tcagcctc 125100

ttttggcatt gattcatcca cttcttccta agacgggatt ctatctctaa ac

#aactctgc 125160

tttacagttg ttgggttttt ttttaaccaa gttatgtctc tttatattct ta

#cccactga 125220

cttaaattct aatgcatagc aagcttaacc atcttcatta tggtgaatct ac

#aaatacat 125280

gaagatttcc tctgctgccc acactctcca taggcttttt cttatccata gg

#tcttctca 125340

tccatgccct ctatttcctt cagttctatt aaggctcttg ttatatgacg tt

#ccaccctt 125400

tctccaacgt caaacatact tgtgctgtgt ctcattccct ccaagccttt gt

#catggagg 125460

aaaaaaacga attagttcta aatctgatat tggttgataa ctaatctaaa at

#tacaatca 125520

tatattgggt cctgttgtca aaggagtgaa taatgggaga atttaagact tt

#aagacttt 125580

ttaaccagag aagtgaagga aagtttagag aagctaaggt attctttaaa tt

#tcattcta 125640

ttttaatgct agaactttaa atctgtattt aaagaattac atgaatttac ta

#ttatggta 125700

acattttatt catttatcaa atgattgatt ccctctaaaa tgtaattcaa aa

#tgtaaaca 125760

ttttggtgaa atcttatgct tacaatttcc attaaaatct aaactctaca gc

#atgttaaa 125820

gttttacttg gatttacaaa atgatgcata tatgcattta gatatttaca tt

#tcatcact 125880

actctgataa tcaaatgcca tcaagcagga caaggacaac tggttgtatc ag

#tgacctat 125940

tgatttgtat cattttttat tcaccaataa gtagatacaa atcaacagct ca

#tattgtct 126000

aatgttccat aaggcatgac agtacaggat atgaatataa ttaagaagaa aa

#acagacaa 126060

ttttagtagg tgtagctgaa ccacacagat tatgtaagca aagtaatttt cg

#caaacccc 126120

cagtgtcccc ttgaaatatg gtaggttgtc agcatacaac tatgagcaaa tg

#ataacgtg 126180

gtatgagcaa taaactagga agcctgaaga tatatattct gctgtaatca ag

#tgatgttg 126240

taatataata aatcttacaa caacgtcact atgacccaat gtaatgatat gc

#ataatcat 126300

tgctgagctg catggtaagc aggctcagat ggaaggcact ttacaagaag gc

#ggatttct 126360

atattggggc tggcagtgta ctgtagcact cagagaaatc tcctttgctc ag

#gatgtcaa 126420

gaacagatgg gaacagatgt agcaatgata ctgcagtggc cctcactttc ca

#cctacgta 126480

ttcctacaat cttcacctta gaaagaatgt ctggtatatc aatttccgcc ta

#cccccaaa 126540

ttttattcga aagcacttcc aattgaaagt ttatgaacac ttgtcccagg ag

#caaaagac 126600

agaggtcatc tatgaatgga ttccgggttt caatttcttt ggaagctatg gc

#aaagggaa 126660

gagaactata gagggaggta ggaaagaaag caaaataact agtgttcaga ta

#gaaacatg 126720

aaaaactgaa gtctggggaa gagacagagg aggaccagca tatggtctga gg

#gatgtatg 126780

ttaagactgg gaccctccag cccagggttt ataactaggt taacatgcca at

#gtgtgttt 126840

tcctctgccg ttggccattc tcgagaatgg tgctcccaga gttaggactt gg

#aaaggctg 126900

gaagattttg atgaaggata gactgtgaaa gaggtaggaa gaagtgatat tg

#cagcccca 126960

taatctgcca ccaactgtac aaatgagttt agaaaggttt tcaagagagt ca

#aaaatgaa 127020

aatactgtga ttttaggtat aagaggaagg cttataatta attttgagga ag

#gctcgtca 127080

aaattatctc tcctgtcaat ttcagatgcc tgcaattact ttaatttgat ga

#cagctttt 127140

aacacaacta gagattaaag gctatcatgc aaatggttgc agtaacatta ga

#aacatcag 127200

aatttgttcc tatgttgaca gagcattata atannnnnnn nnnnnnnnnn nn

#nnnnnnnn 127260

nnnnnnnnnn nnnnnnnnnn nnnaatttat taatattgat cgtcctctct tc

#tttgatcc 127320

ctccaaactt tgtatattta gcatagcctt tgtcagattc taactcacag tg

#atctcatt 127380

tattaactgc cttctctgtg tgaggaagag cataaggtac tgggcactac at

#gaggataa 127440

gcatgtatat ttcctcttat cttagtacca cgtaaggatg gagattcagt ct

#tcatgatc 127500

ttactatcaa tccttcaata tgaattgagg acccctaaaa cacatacata tg

#aaaacaca 127560

aacacacatg catgtttata tacatcattt acaaggattt gctagacagt tt

#gggggata 127620

ttaagatgaa gaatctctgc ttttaatgac atcataatca tacaaaagga aa

#aaatatat 127680

atatataaac acatatatgt gtgtatacat aaatatattt gtatatgtgt at

#atacagtt 127740

atattttata tgtctatatt ttatatatgt atatatataa tataaacata ta

#tacatttt 127800

gtatgtatgt atctagatat gtatatgcat gtatataaat tctcccacta tt

#cactcctt 127860

tagcaatagg acccaatata tatattgtaa ttttggaata attatcaaac aa

#tatagaat 127920

atggtgatat atttttatat gccgatatgt atacatttgt atataaacat at

#acatatat 127980

gtgtatatgt acacacatat atgtacacat acacacatat acttataggc at

#atacaaat 128040

atatcaccat caatgcccaa cacattttct ttagcataat aaagcaattg ag

#ggtgtttg 128100

caggagtaaa tataaactgc acattttaca ttttttttcc ctagagctta ta

#tgggtaaa 128160

taaagaaaac ctgttcaagg acattgatag gcactaaaat gtcattattt cc

#tgtataat 128220

atggataaac tttaacataa aaaaatctgc aatttttgga aacctttaca tt

#tatagagc 128280

acttcaaaat tttcaaagca atttcacatt ccttatttta caaatagtat tc

#aaaatgac 128340

tctctgaggt gtgcaagaaa ataatgatta tctctaattt ataattagga aa

#ctgaagat 128400

tagcttattt aagtagcctg ctctacaggg tacactatta gaaaggacta aa

#gtcaaaac 128460

cagtgttcta gtctctaggc ctatacattg ttttcattat tcaaacttac tg

#ccttcttc 128520

tatacaaatt taatgaaata ccttatcttc actaaactta atgctagaat ta

#ttgagaaa 128580

gtatgcagat aattaggttt gcaccattca acattcacaa tagttaaatc tc

#aaaggatg 128640

aaaagaagga ttggtctgac cttctccatc tcatatgcta ttctaaaact aa

#ttgtatct 128700

gcatatacaa attcactgga tataactgaa taactgctgt atgagattag aa

#taaagcat 128760

aaaaatattg atttggaagc aatatttaaa ttactttttt agcatgtagt tc

#cacaatac 128820

ctgagatgta gtaggcattt aataattgag ttcataaaaa ggaggattat at

#ttagatgg 128880

gtaaatacat atgcttcaga gttcaaatgg acctgagttc aaattccttc tc

#tgcatttt 128940

tgtagctgta tgacctgaaa ctttctgagt gaagtttcta cactgataaa gt

#aggaataa 129000

taatcaaccc tactttattc attgctgcta aatcatattt caattattca ct

#gaattaca 129060

cattggaagc atttaataaa tacatgttat ttttattgct gttgatgttt ca

#tggtagta 129120

gattctacat tttcctggct gataatccag aggaaaatct ctgagctaat tt

#aaggactg 129180

caatgaaaag tggcatccat gggtaaaggt cataatgaaa gttgacctgt gg

#aatgaaac 129240

ttacactttg ttccatgtat cacagagctt taaaaaccag taaactctat at

#tccaatta 129300

aagggcaaaa gtccaggcaa gaagtttcct ctcagaaaaa ctcaaaagtt tg

#cacacaca 129360

tattcaaagg tagaagcaga aatagcaaac agaattgaca tactttcttc at

#tttcataa 129420

gatacaatgg aaatatctcc aaaacacctt tgggcaaaca ttttacctgg tg

#ctttacca 129480

ttttctgaaa taaattagcc attacaggaa gaaaacttaa atgtgtctta gc

#ttctttac 129540

atgagaatca aggggggaaa tgtgaccata taaagatata tttaaataac ag

#ataataca 129600

tagatatatg tattaaaaag aaatataaaa taatatttca aatcctggaa aa

#ctgagatc 129660

atataatgtt agttttgtaa ataagttgta acaagattgt ataggaataa tc

#ccaattat 129720

ttatatatgt gtatgtatat aaaatataca gtataatatt tagtatacaa ta

#tgtgatat 129780

attgtctata ttactctgtg tggaacctac tcctccctta caggtactgg ct

#ttctggcc 129840

ttgctcacca gtgggtctgc ataccctccc gtacgtactc agcatagaga ag

#ggtcaagt 129900

tgcctcaatc ctcagtgcca cctccacatc attctctatc cctctgccct aa

#aattgcca 129960

gcttgaattc atgctatcaa gcataggaca caccattgct ctttttggaa gt

#taattacc 130020

atcccctaag tcactttctc ttgtttctta aagatttcac tccgggatca ct

#gcttctct 130080

ctcatcactc ttctgtcata attattgatg atgtcaaaat tcatataaaa ta

#ttgaccca 130140

tagccctgct catcactttc tggacctctt ctcttgcagt gacttgcctt cc

#acatgacc 130200

tcatcacttt ctgccatgtt cacaacctag accttttcac caccaagaat tg

#tagcctct 130260

ccataatctt gattgcagat gtctcactat ctgtccaaat ccttctttcc ag

#atcactgg 130320

actctgataa ttcttcaacc ctgctctgcc ctacagctct ttgattctat cg

#aattttcc 130380

atttctccta acccaatcaa gacttcattt tttctcttgc tcagcttgaa ct

#gcatgcct 130440

atttgtttcc tttcctctat tgtatatatc ctcaactctc aaatttatct ct

#tatagtct 130500

cgaccttttg ataaatcccc aaactttgct aaataaaacc ctccatggat tc

#ttctctgt 130560

gttactagaa gtgtattgga gaaaaattca tgatcacgcc aaaatatctc ac

#tttaaact 130620

catggccact aacctcaaaa acacagcctc ctaatctatt caatctccct tt

#cttctagg 130680

agattctttc tccttcttct ttcttctctg gcctctaaca ctatattcct ca

#ttttcacc 130740

tacatctgat agccttgttt atatttcact gataaaacaa aagcaattaa aa

#gagaacat 130800

tcacaaggtc ccctacttgt ctgcatctgt gtccatatac tcagtctttc tc

#ctgtggct 130860

gcatatgaac tgtcctagtc cctgataaca gccaaccctc tcacttggac ac

#tacatgac 130920

ctctcccttt gcctctcaag aacatgggtt gaggaattct cccttctgca tc

#atcatttt 130980

ttctctttta gcagctaaac aaatctattg taatttctta catcataaaa at

#atcttgat 131040

attataggtt tctctctact tctttattgt tctttttatt tacattatag cc

#ccttgaag 131100

aattgtcaac acttactatc ttcaattccc ttctcttatt tcttcagcac cc

#tcaaataa 131160

gacttggata ctaagcaagc acaacactga atcagctatt ttcaaggtca tc

#aattaact 131220

actcagaaaa ttagccttaa gtctcagtct ccaatttatt tgacttttca gc

#agctctga 131280

ctctttcatc ttagtctcat aggttctatc tcatcttcta gacctgcaaa ca

#aaataatt 131340

tagagctcag tacttgaatt tactatctct ttctaaactc tttttcttgg tg

#attgtaag 131400

agtcagggtt ctctagaggg acagaactaa caggatagat gtatatatga ag

#gggcgttt 131460

attaaggagt gttgactcac acaatcacaa ggtgaagccc caaaataggc ca

#tctgcaag 131520

ctgaggagca aggaagccag tctaagtccc aaaatctcaa aagtagggaa gc

#tgatagtg 131580

cagccttcag tctatgacca aagggccatg gcaaattact ggtgcaagtc cc

#agagtcca 131640

aaagctgaag tacgtggagt ccgatgttct agggcaggaa gcatccagca tt

#tcccagtc 131700

cactgactca aatgttaatc tcctttggca ataccctcac agacacaccc ag

#aaacaata 131760

ctttgtgttc ttcaatccaa tcaagatgac actcaatatt gaccatcgca gt

#gatgtcat 131820

ccaattttca tgactttaag taagagatat gagctgatta ctttcaaatt ta

#tgtctcta 131880

gtttggactt cttactgaat tctaaagtca tatatctaat tgccttcgtg gc

#attcctac 131940

ctgaatatct aatagtgatt tcaaacataa tatgtccaat gtgagttttt ta

#ttttccct 132000

gcaaatctgt tcatactaaa acctcaaaaa cacaggcagt aaaagcaaaa at

#atacaaat 132060

gggattatat caaagtaaaa atcatatgca cacaaaggaa acaatcaaca ga

#atgaatag 132120

acaatctgca aaatgggaga aaatatttgc aaactattca tccaacaagg ga

#ttaatatc 132180

caaaatatac caggaactca actcaatagc agaaaaaaaa tccaatttaa aa

#atgggcaa 132240

atgagctgaa tgaacatctc tcaaaagaag acatacaaat ggccaacagg ca

#tatgaaag 132300

attgctcaac atcactaatc atcaaggaaa tacaaatcaa aaccacaatg aa

#acaccatc 132360

tctccccatt tagaatggtt attatcaaaa agacaaaaaa ataacaaatg cc

#agcaagaa 132420

tgcagagaaa gtggaattat tatacactat tatacactat ttagttttcc tc

#agaaaact 132480

aaaatacaac catcattatg acccagcaat accactactg ggtatatatc ca

#aagggaag 132540

aaaatcagta tgtcaaaggc atatctatgc ttacgcagta agtgctgcag ca

#ctattcac 132600

aatagatgag ataaagaatc agcctaagta ttcatcaaca gatgaatgaa ta

#aagaaaat 132660

atgctgtata tacgcaatgg aatactattt agccatgtaa aagaataaag tc

#ctgtcatt 132720

tgtggcaata tggatgagct tggagaacat tatgataatt gaaataatcc ag

#gaacagaa 132780

aaataaatac cacatgttct cacttatgca gaggctgaaa aagttgatct cg

#tgaaagta 132840

gagagtagaa tagtggttaa aagctgggaa ggggaagagg tgagagtaag ag

#attggtta 132900

acgaatgcaa aattacagct agataggaga aataaatact ggtgtctata gc

#tctgtagt 132960

gtgactataa caaaccacaa tttattgtat attttcaaat agctagaaga gc

#agaatttg 133020

atgttcccaa cataaagaaa ttataaatgt ttaaggagat ggatgtgctc at

#taccctga 133080

cttgagtatt acacattgca tacatgtatg aaaattttca cactgtattc ca

#taaaaatg 133140

tgcaattatt atgtgtcaaa ataataagaa aagattatta aaaactgctc at

#ctggagtc 133200

ttccccatct tcctttggag tcgttattga tttctctgtt tctctcatac ct

#catatcaa 133260

atctattagc aaattcagtt ggttttgcct tcaaaatgta tccatatctg at

#cacttctc 133320

accatctcca ttgatatcac ccatgccacc aatatttctt ggctgaattg tt

#acaataac 133380

cctctaacta ttctccctcc tttcaccttt taaactccca taggttggtc ta

#tggaagcc 133440

cacgtgaaac tgttaaacca cacactatgt ttgaaacctt tcagtgactt tc

#tgtgtcat 133500

tcagagtaaa aagcaaagtc ttataattac tttttaggac ctaaagcacc ac

#ttatactc 133560

cctgcttttt ctagccatta tctgttactc ttccccctca tttactctac tc

#caggcacc 133620

tgctgttcct agaacattcc tgacaccctt ctcctttaag gtcgttggac tt

#gattttcc 133680

ttctacccac aattcttttc ccccgaatcc tgcaggcctc acttctttcc tt

#cttcaaaa 133740

ctgtcttcac attatcacca gtgatatgtg aagtttggag atgggctgga ga

#acactatg 133800

ataagtgaaa taagccagga acagaaaaat aaataccgca tattctcatg aa

#gtatttat 133860

tttttctgaa taacctattt ctgaacagcc tattttctga aaagcctatt tt

#ctgaaaac 133920

tttctctcat agcccttatc acttttataa attctatgta atttgcatac at

#aatataca 133980

ttaatagaca atgtctattt ctcctaatga taaaataaac gagggtagga at

#ttcagtgt 134040

ctttggtcag tgatgaaccc ccagctccta aaatagtgcc tggaatgtaa ta

#gtcactca 134100

caaatattga ttcagtggag aatgtgcata tttaaaaaat ctgtaaagaa at

#caaccaaa 134160

atgttaatgg tccttcactc tggatagtgg gattacaggt gaattctact tt

#ctattatg 134220

tatttttcta aattttcaaa atattctaca ttaacatata ttatttttaa ta

#agaaaaga 134280

tccctcacac tttaactaca tatttaggtc tttcggttga gactggaaag ac

#agaaaagc 134340

tgcagtatac tgtgtattta agagaatcaa gattttctac aagcaaatgt tc

#ctggcttg 134400

cactgtaatt tgggaaaatc acctaaagtg cctcctcatt gttccttaaa gt

#aaaataaa 134460

cttgctggat tacattttag agtccctgga aaatttaaat atatgttatt tt

#ttgtatat 134520

tactattctc tgactactga gacaatttca atgtaaaaaa gtaaatgtta cc

#ttttattc 134580

catattcctt aaagcatctt cctgtttgaa atagatgtca ttccattact ac

#tttttaac 134640

ttatacatta cctttcttta aaagaaatcc acagatactg ttcacaatta ta

#taaactca 134700

agtgtcatgc ttttatgttc caggtaaata gaccaaattt cagagaaatt tg

#ataaatat 134760

acacaaggat gtcataatag atttaagaca gatctcatgt cctatgagtt ta

#ctgtatta 134820

gcaaaatgaa acttcatatt accatgtttt tcttgggtca gaactccaga ca

#gtaaatgc 134880

cactagacta atgactaatg ccacagttta agtagataag taatttctta ga

#ggaagagt 134940

gtacatatat ctgcacaacc aataaataca tggcagaaac atcatggagt gg

#gtttagag 135000

agctggttct gggctcaacc tgccttacca attttgagat cttggcaagt ta

#cttcacct 135060

ttctaagctt caatatcttc atctataaaa tgagcataat attagtacta at

#tcacaatg 135120

attttataag aatattgaat ataagatgct tagcaaactg ctacaaagac tc

#agacttaa 135180

gacctttatt aagttctgtt attattgtaa atattattat gtagtcctta at

#gttttatt 135240

caaaagttag acataaattt tgagaaccat ttgttgtgta gtatatcaga tt

#gtgaggat 135300

aaatttagac gttggaaatt ttgagtattt aagattatct agtatttacg gt

#attctaaa 135360

atattaggta attttacaac cagcatatgt ttcatgcatt gatcgaaaac ta

#aaacactg 135420

tatctgtgaa cacagtgatg cagtgtttgt aattatatcc ttctagggtt at

#ttatgtcc 135480

aaatgaattt attgctactc aaggtccact accaggaaca gttggagatt tt

#tggagaat 135540

ggtgtgggaa accagagcaa aaacattagt aatgctaaca cagtgttttg aa

#aaaggacg 135600

ggtaagttat ttgaaaatgt tttacaaatg ttgttttacg attgtgttaa ca

#tatgtgtg 135660

aatatttcat ctaatactgt gagtcatcaa taacctggac atctataaag ta

#attttaac 135720

ttagtcgtaa taactgtggt atacatatat atcaatataa caatgacgct ta

#tgactgat 135780

gattttctct gaatgcagat cagatgccat cagtattggc cagaggacaa ca

#agccagtt 135840

actgtctttg gagatatagt gattacaaag ctaatggagg atgttcaaat ag

#attggact 135900

atcagggatc tgaaaattga aagggtaaaa aaaaaagggg gggacgagag aa

#catgatat 135960

aaaatatgat tgatctaaat gtctaaaata aaattaattt ctagaactat cc

#ctttcaag 136020

gatacctgta tattcaacaa tgcttttgta ttgtcttctg aacagaattt tg

#aatcgata 136080

tccaacttta gtatcaatgt cactgtattt gttccagatc actctagtta aa

#gtctgtat 136140

taaccaatta gcatcacatt cttaggttga caagagcaga aaaaggagag aa

#aatgatga 136200

gatcactagc tttattttat ctaatgaaga aactgtaata tctgacttga ga

#cagcaatt 136260

tcccaagtca ctcatctcct gaatcctaat aatttgattt tctatttaat ct

#gcagccag 136320

atagaaaagg tagtatggga tctcacttta tgagatcttt atgggatcac tt

#tatgggat 136380

ctcagtttat ataaatgcct atacacacag aagatgatat aaggatcttt at

#acttttca 136440

cataacagat gctcaatacc tgtgtatgga ataatttgtg aatgtgttca tt

#taagtttt 136500

gggtcaaaag tgtttcaata cctattattc tgagtgctac aaaatggcat ac

#tatatttg 136560

aatattaatg tcctatatta acatttattt ccaagctttc ttatgttttc at

#tcatattg 136620

aaaggcaatt ctctttattg taacaataaa aatctctctt ataggaaata at

#gaaaacat 136680

tttatttggt ttggtaaata gtcattttta aaagatcacc ttcaaaaact gg

#gactattg 136740

ccttcaacct tcattgtgga acttaaataa ttttgtcatt cattaatccg tc

#cctttgtc 136800

tagcatgggg attgcatgac tgttcgacag tgtaacttta ctgcctggcc ag

#agcatggg 136860

gttccctgag aacagcgccc ctctaattca ctttgtgaag ttggttcgag ca

#agcagggc 136920

acatgacacc acacctatga ttgttcactg caggtgagaa agtgatcaga aa

#tggccttt 136980

gaacccattg gtctttttat tattaaaatt ccattggtta ttttttataa aa

#tgttcatg 137040

taaatttctt ccagcttgcc gtcttcagag atttcacatt tagcatttct ag

#acacattg 137100

gtatgattta tgttttctga catgatagat ctaaaccagt cttgactcga gt

#ctttttca 137160

cagttgaagt ttggagatta gaggaaaatg tagtatgaat tctacttaaa tg

#agatactc 137220

agaataggta aataaataga aacagaaagt agagttgtgg ttattagtga gg

#agggagat 137280

gaaaaattat tatctaatgg gtacagacct tctatttggg atgatgaaaa gt

#tctggaaa 137340

tatactgtgt tgatatttgc acaacattga gaatatactt aatgccactg aa

#ttgtacat 137400

gtaaaatggt caaaatggta gattctctgt tattcatctt tcaccacaat aa

#aaagattt 137460

ttttaaagta acaataactt ttaatatttt taacaggagt gtactataat ta

#atgtggtt 137520

aaatactgta cataagaaaa gataaattca ttcagttttt aaacttttat tt

#taaaaaac 137580

tcaatatgta atttaaatga ttatacattt tccataaatt tctggatttt ta

#aaattata 137640

agactaatac gtaaatgctt gctcattata aaatatatga acaatgctta ta

#tttataaa 137700

acatgtagaa caaaaagtaa aagcctcatt tttaccttct caattttaaa tc

#cctccata 137760

taggtataaa gcttaatata atagtatata ctaaaaacag tataacaatt tg

#cttctaca 137820

gtcaatgatt gattttatgc tggcatcact gatgattaaa acctttgatg ga

#cagtagct 137880

gtcttcaatt tctctgtatc atgcaaatac actgccatgg gcactaaaag aa

#aagtactt 137940

tctccttttt agcctcaaaa agaatagagt ctcctccttt gtgatttaaa ta

#agagataa 138000

gaagaaagtt gttcatatta ttgaccatnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 138780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca tatacctgtc tt

#tgagtatt 138840

gatgtaattt tttaaatgaa aatagttttt caattttatt atctatagga gg

#caaaataa 138900

attctagaga aagaaagtga aaaggacaat tgcaacacta tttttcaaaa tg

#aaacaaag 138960

aaaatgctat ataagctaaa tattctacat ttgtaacatt tagcatttct gc

#tggtaact 139020

gaatatttgg tcaatacaga gtctctggta ttaagaattt tcaatgattt ta

#aaaaaatc 139080

tctatacttc aacaacacac cctaaaatat taagaaataa gaggttaagt tc

#cactgatt 139140

aaagaaagac aaactcaaat atttgatagc atattaatga taatcatctt gc

#cttgttta 139200

aacacaatct tggtaaccat aaaaaatcca aagacactcc aaagaaaatc tg

#cctccaaa 139260

taagagaaga aactattaga atttattgct atcatagctc attatcttta tc

#cccatcaa 139320

aatgaacaac cctttgctga ataattttca tgtaatttac ccttcctgta gt

#gctggagt 139380

tggaagaact ggagttttta ttgctctgga ccatttaaca caacatataa at

#gaccatga 139440

ttttgtggat atatatggac tagtagctga actgagaagt gaaagaatgt gc

#atggtgca 139500

gaatctggta agatctctaa acctgcactg cattctaaag ttctagaatt tc

#cacatggg 139560

agatccttag tggcagcaat ctggatggac atgagcttga agctgtggac ac

#cttctttt 139620

cctacattat aagccttttg gggaggattc gggagggcag ctgatagaga tt

#ataggaga 139680

actaatgccc acatgccata gtcaccctgc agcattgtta ctgatggctc at

#cttaactt 139740

gttatactga taggcatgta ggcagtaaca taaaattgat ttatctttat cg

#tttagcaa 139800

ctttgggata tctggaaatg aactcaaatc aatatctttt gaatatcatt at

#cttttgaa 139860

aagttataaa tgggaaaaca gtttaaaata ttgactgtaa taaagttcta tg

#ggttttac 139920

ttctccatat ttatccctat tgcataccag tactaataat gattattgta gc

#acgctatc 139980

aactattaac tgtgaggttt ttgtttgttg ttttggctta taggcaaaaa at

#atttacaa 140040

aatatataca attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 140940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 141960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 142980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 143040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 143100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 143160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 143220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 143280

nnnnnnnnnn nnnnnnnnnn nnnnnnnaac aacaacaaaa ccaacaacaa ca

#cattgtag 143340

ccccaggaaa tttgaaaggc ctttatgaga gaactctcct gtagtgagct aa

#tatcctaa 143400

gatactttta gtgcctcaag gaagtggaaa tttactggtg tgaactcata ta

#ggggaaga 143460

aaaagaaaaa aagtcaacat attttggatg tccagttttt tgtcgggcat tt

#tatgtatg 143520

ttaaatcctt taaatcttac aatacaggat ttatgacaca cactcccacc cc

#cagatgag 143580

gaaattgtgc atcagaagag tttaattctt aaggtaatat ggctctctag gg

#cagaaact 143640

ggaattaaac ttttttcaca acagtatgct gctttcttgg caataacaca ta

#acggcaga 143700

aggaccttgg aacctgtagg actgtctcac agagttcagc ctgctctgct gg

#caaagttt 143760

acacctatca ttctttccag tggagaagat gaaatcagga cagtcagaag tt

#tcacatat 143820

caaatgtgac ttcacatatt tttttaaata ctagaactca taaatttaaa tg

#atttccaa 143880

aaagattata ttgtgtcaac atatttctgt caataatgta attcactgtg tc

#atgtatgt 143940

ttgaaaacac actcttggaa ttacctcgag aagtaactta ctagcaattt ca

#gtagaaat 144000

tttattgctt tataacaaca cttcaattct taccaaaatt gaattctata aa

#ctagatca 144060

tccacctcat ttacaaaact taacacctaa tgacatttga attttcttta aa

#ttacatct 144120

gcccttaaat ggtaaaggtt gactagctgt gagaattaaa tgagactaag tc

#aacaaaca 144180

cttattatac aactactatg tgcccggcac tattataggt aatcagaata ta

#gcagtgaa 144240

tatgacagag ttctgccttt ataaaactga cgttccagta atgagatgtt ct

#tggaaaca 144300

ttttgtaatc cacaaagaaa tagatattcc taataatgac aaacaatttc tg

#aagacaat 144360

ttcaatagag gagttccaaa aggttttgag gtacagtagc aatagatacg aa

#tataacct 144420

ctgaggctga tcacttttga gaatgttcta tttaaatcat tgtcaatttg aa

#tatatgtc 144480

ttaaacattt gatgatattc ctttaaagtc agatatgttt gttatgtgca aa

#tgagggtg 144540

atttgaaata tacttttttt tttagcttta actacttttg ataaggtcca aa

#ctcagaga 144600

tgctagtagg ttattgaatt atattgaaaa catttaaagg atccaaatgg ta

#ctgaattt 144660

agcccaaaca ttcagatgca atggtaggag tccttgtcca gcacctggat gt

#ttgggtac 144720

ttcaatgacc cactgccttg tatttacaaa tcaggaccag atacttgatc tt

#aagcaggc 144780

cacatatcca ggtgactaac agatttattg gttaaacata ttttaaatgc gc

#tgatgatg 144840

tatagatatg ctgactcaca gatttcaaaa gtaaatttag catttgtatt cc

#aacagtca 144900

ttctaacaag aaaactgtaa gagaatttac caattaggtc taacaggaaa aa

#aactcata 144960

aacaaattta tgtaatataa ttttctactt cttatgataa cagcaagaaa ga

#atatatta 145020

atacttggtg tttagtgaca agtgttagaa aaaaacttga agcttcaaga ga

#ccacagga 145080

atttagaaag cctcctattt gaaatggtag aaaatcatat ctatactatg at

#aaattctg 145140

tgtctgtaac ttagctattt atttgatgaa ttcagtactg cttttagctt ta

#acaatata 145200

actcccttta tgaaactctt catcaatata tttgtttaac cactctgtct tt

#ggtgtcta 145260

ggcacagtat atctttttac accagtgcat tctggatctc ttatcaaata ag

#ggaagtaa 145320

tcagcccatc tgttttgtta actattcagc acttcagaag atggactctt tg

#gacgccat 145380

ggaaggtaaa cagaaacaac agtatatgcc cagcttacta gtttaccacc ta

#cggtaaga 145440

acataaattt cagaataacc atatgttaaa aatgtttaag aagctggatt ag

#tgcacaga 145500

tcaggttttt tttctttaac ttttctctaa tccaagttgg gctaataata cc

#ctttctgt 145560

ctacattata tttttatcat gaaacatttc aattttgaac tgttaacttc aa

#cactctct 145620

tgtaacatgt tactttctgt tataggtgat gttgagcttg aatgggaaga aa

#ccactatg 145680

taaatattca gaccaaagga tacaattgga agagattttt aaatcccagg gg

#ccaaagtt 145740

accccctcat tcttccgaat tgaaatgtgc aaccttaaag aaatatctat gc

#ttctctca 145800

ctgtgccttt ccaaacggat tgaacatttt aagnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 145860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 145920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 145980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 146940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 147780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tatcttcatt agtttcttgt ct

#aagacttc 147840

atagatacta gttactctct ggggtccctg aagcaatagt attaaccctc ac

#acaaatca 147900

gtaaatgtga gtagtagttg gttagacgga tcagtcatgg tagatttttg tg

#tattttaa 147960

tgtagcagat aggagattca agcttttttt ctctcaagct tgagaataca ga

#gggcatag 148020

gtctggctta ccttgtaaaa aatgccagca gctaacaatg aaattctacc ca

#acacaggc 148080

tgggtatttc tctgattttt tgccttgggt ttacagtatt cctagagtta cc

#agaaaact 148140

atagtggaca attagcggtg gatgccaaga gaatgcttgg aactttgaga at

#gttggggt 148200

ggacattaat caattgatat aagctttggg tatggaggac aacgttatgt ta

#taatcatt 148260

agaagaaatt tcaaaggcga taaagaaaaa ctatttcaga aacgctcttc cc

#tgaaacac 148320

caagaaagtg acctattatg ttaatatttt tgttatatgc aatgtgccct gt

#tagttttg 148380

ttagaaaatg tacattttat tatatccatt ttcaaatcgt ttctggtagt gg

#ggttttaa 148440

aatgataaat gaggttcaaa attaattcca gcctcctttc ttttagaaac ag

#tgttagat 148500

tgaatctgca tcaggcgtgt tttcacatgc ttggcttcat aatctctctt cc

#tcccccta 148560

tattgtttgc ctggaatctg cactaaagat aaggcagagt gcaaacctga ct

#cattggca 148620

accaatcaga agaactttat gtggaaaact cccttcgagg aggtacaggc ag

#catgaaca 148680

aaatttttga aaaagtggaa gcaaaggtag aaaaatatgg ttgaaatggc ta

#aaacaatt 148740

ggtacttgtt ttaaaactat atttcatttc tgatatgaaa ccttatcttt tc

#ttttaaag 148800

aaacacctaa caaaatattt atcagatcag caccacagta aagggaaaaa ga

#cattaaaa 148860

attaaaaaag ataaaataac aaatatttat cagaaatgct cacccttcaa aa

#aatctgga 148920

agattttgat tatatatttt tccaattatc ttctgtttgg taaatttcca ag

#taattgga 148980

taaatagttt atatttactt tgttttaaaa tgactcaaat tttcaattag ag

#cataagct 149040

ttcaaaaaca atctggtcaa ctagcagact tttagcaaat aagacatatt tc

#agaaacag 149100

aaaattaatt gttatattat ttatgatagt tatacctaaa acctaggtgt tg

#ttaaatat 149160

ttacatgttt aacacccaag tatacttaga gatcatttat tgtactcagt ga

#tttctaac 149220

aacatgatta ttttggaact tgaacctata ctatttgttt tcattttttt ga

#aactttag 149280

gagaataact ttattttaaa cctctatttt tcaatatcag aaccagaaca ac

#ctgagaaa 149340

cttagagcct tcaatatttc cacacattcc ttttctctgc actggagcct ac

#cctctggt 149400

catgtggaaa ggtatcaagt ggatcttgtt cctgacagtg gctttgttac ta

#tcagagat 149460

cttggaggtg gagaatatca ggtatagttt tcattattgt acttgccgag cc

#tacttgta 149520

tttatatttt gctcctaata ggaaagttct ttattttatg aaacccatct ac

#cacaaaaa 149580

cttactcctt gttgggtttt tgaaagcata agttgaagac aaaaacgttg at

#gtcaaact 149640

gatgagtgtt aagtttcagc attggtggac tgttacctta gcaacatcta tg

#ctgctttt 149700

tttttttttt tttttttttt aagttcaccc tgaacctaca gccagtcatc ca

#agggttca 149760

tgaatagttt aacaaagaaa aggcagagct attgagtaat acgggctcat ta

#attgtgta 149820

cttgccagaa ggatctgtct ttaaatcatt aatgcaggca acatttctct ct

#agagccat 149880

caatgtgatt ctactggctg aaaaatgtaa taaagatgga ttttcttatc at

#ttttcttt 149940

tactttttat tgggacttca gagacacagg tatttcgtat acactcttta aa

#aacaaggg 150000

ctaagtcatg ggctgtagat ttctcaagac ttgaatagtt gttccttgtg ac

#agtgaact 150060

aggatagata gaaatgctga cttaggctgt gataacgcag tacgttttgt aa

#gtttttat 150120

tttaaagtca tttggtaaaa agttatataa catatttgta tcttacaata at

#atggaact 150180

tattgtgatg ttataaacag tgcagagtta tatagtgaag agttaatttt tg

#ttatagtg 150240

atagatttat tttagcttgc ttgctttcca gaaagaattt taatgcaact at

#ttgtttgt 150300

gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 150960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 151980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 152940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 153960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 154980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 155940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 156960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 157980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 158940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 159960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 160980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 161940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 162960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 163980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 164940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 165960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 166980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 167940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 168960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 169980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 170940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 171960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 172980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 173940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 174960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 175980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 176940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 177960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 178980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179580

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179640

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179700

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179760

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179820

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179880

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 179940

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180000

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180060

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180120

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180180

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180240

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180300

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180360

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180420

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180480

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180540

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180600

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180660

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180720

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180780

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180840

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180900

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 180960

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181020

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181080

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181140

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181200

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181260

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181320

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181380

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181440

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181500

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181560

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181620

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181680

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181740

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181800

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181860

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181920

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 181980

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 182040

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 182100

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 182160

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 182220

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 182280

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 182340

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 182400

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 182460

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn

#nnnnnnnn 182520

nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nn