DNA sequences from photorhabdus luminescens

This application claims priority from U.S. Provisional Patent Application Ser. No. US 60/191,806 filed March 24, 2000.

This invention was made with United States government support awarded by the following agencies:

USDA 97-34340-3987

The United States has certain rights in this invention.

As reported in W098/08932, protein toxins from the genus Photorhabdus have been shown to have oral toxicity against insects. The toxin complex produced by Photorhabdus luminescens (W-14), for example, has been shown to contain ten to fourteen proteins, and it is known that these are produced by expression of genes from four distinct genomic regions: tca, tcb, tcc, and tcd. WO98/08932 discloses nucleotide sequences for many of the native toxin genes.

Of the separate toxins isolated from Photorhabdus luminescens (W-14), those designated Toxin A and Toxin B have been the subject of focused investigation for their activity against target insect species of interest, for example corn rootworm. Toxin A is comprised of two different subunits. The native gene tcda (SEQ ID NO:1) encodes protoxin TcdA (see SEQ ID NO:1). As determined by mass spectrometry, TcdA is processed by one or more proteases to provide Toxin A. More specifically, TcdA is an approximately 282.9 kDA protein (2516 aa) that is processed to provide TcdAii, an approximately 208.2 kDA (1849 aa) protein encoded by nucleotides 265-5811 of SEQ ID NO:1, and TcdAiii, an approximately 63.5 kDA (579 aa) protein encoded by nucleotides 5812-7551 of SEQ ID NO:1.

WO 01/11029 discloses nucleotide sequences that encode TcdA and TcbA and have base compositions that have been altered from that of the native genes to make them more similar to plant genes. Also disclosed are transgenic plants that express Toxin A and Toxin B.

We have observed that heterologous expression of Toxin A does not afford the level of oral toxicity to insects that is observed for the native toxin. It would be very valuable if means could be found to enhance the level of toxicity of heterologously expressed Toxin A.

The present invention provides nucleotide sequences for two genes, tcdB and tccC2, from the tcd genomic region of Photorhabdus luminescens W-14. These sequences were not previously known. We have discovered that coexpression of tcdB and tccC2 with tcdA in heterologous hosts results in enhanced levels of oral insect toxicity compared to that obtained when tcdA is expressed alone in such heterologous hosts. Coexpression of tcdB and tccC2 with tcdA or tcbA, or with any other functionally equivalent toxin gene in the same family as tcdA and tcbA, to provide enhanced oral insect activity falls within the scope of the invention.

SEQ ID NO: 1 is the DNA sequence for tcdA from Photorhabdus luminescens W-14.

SEQ ID NO: 2 is the amino acid sequence for TcdA from Photorhabdus luminescens W-14.

SEQ ID NO: 3 is the DNA sequence for tcdB from Photorhabdus luminescens W-14.

SEQ ID NO: 4 is the amino acid sequence for TcdB from Photorhabdus luminescens W-14.

SEQ ID NO: 5 is the DNA sequence for tccC2 from Photorhabdus luminescens W-14.

SEQ ID NO: 6 is the amino acid sequence for TccC2 from from Photorhabdus luminescens W-14.

SEQ ID NO: 7 is the DNA sequence for tcbA from from Photorhabdus luminescens W-14.

SEQ ID NO: 8 is the amino acid sequence for TcbA from Photorhabdus luminescens W-14.

It is preferred for the nucleic acids according to the invention to comprise at least one sequence chosen from

(a) the sequences according to SEQ ID NOS: 3 and 5.

(b) at least 14 base pairs-long partial sequences of the sequences defined under (a),

(c) sequences that hybridize with the sequences defined under (a),

(d) sequences that are at least 70%, preferably 80% and even more preferred, 90% identical to the sequences defined under (a),

(e) sequences that are at least 70%, preferably 80% and even more preferred, 90% similar to the sequences defined under (a),

(f) sequences that complement the sequences defined under (a), and

(g) sequences that due to the degeneracy of the genetic code, code for the same amino acid sequence as(i.e. are “isocoding” with) the sequences defined under (a) through (e).

The expression “hybridize” as used herein refers to hybridization under the following specified conditions: 5×SSC; blocking reagent (Roche Diagnostics Inc., Mannheim, Germany), 1%; N-lauroyl-sarcosine, 0.1%; SDS (sodium-dodecyl sulfate) 0.02%; hybridization temperature: 60° C.; first wash step: 2×SSC at 60° C.; second wash step: 2×SSC at 60° C.; preferred second wash step: 0.5×SSC at 60° C.; especially preferred second wash step: 0.2×SSC at 60° C.

“Identity” and “similarity” are scored by the GAP algorithm using the Blosum 62 protein scoring matrix (Wisconsin Package Version 9.0, Genetics Computer Group (GCG), Madison, Wis.).

Expression of the Nucleotide Sequences in Heterologous Microbial Hosts

As biological insect control agents, the insecticidal toxins are produced by expression of the nucleotide sequences in heterologous host cells capable of expressing the nucleotide sequences. In a first embodiment, additional copies of one or more of the nucleotide sequences are added to Xenorhabdus nematophilus, Xenorhabdus poinarii, or Photorhabdus luminescens cells either by insertion into the chromosome or by introduction of extrachromosomally replicating molecules containing the nucleotide sequences.

In another embodiment, at least one of the nucleotide sequences of the invention is inserted into an appropriate expression cassette, comprising a promoter and termination signals. Expression of the nucleotide sequence is constitutive, or an inducible promoter responding to various types of stimuli to initiate transcription is used. In a preferred embodiment, the cell in which the toxin is expressed is a microorganism, such as a virus, a bacteria, or a fungus. In a preferred embodiment, a virus, such as a baculovirus, contains a nucleotide sequence of the invention in its genome and expresses large amounts of the corresponding insecticidal toxin after infection of appropriate eukaryotic cells that are suitable for virus replication and expression of the nucleotide sequence. The insecticidal toxin thus produced is used as an insecticidal agent. Alternatively, baculoviruses engineered to include the nucleotide sequence are used to infect insects in-vivo and kill them either by expression of the insecticidal toxin or by a combination of viral infection and expression of the insecticidal toxin.

Bacterial cells are also hosts for the expression of the nucleotide sequences of the invention. In a preferred embodiment, non-pathogenic symbiotic bacteria, which are able to live and replicate within plant tissues, so-called endophytes, or non-pathogenic symbiotic bacteria, which are capable of colonizing the phyllosphere or the rhizosphere, so-called epiphytes, are used. Such bacteria include bacteria of the genera Agrobacterium, Alcaligenes, Azospiriilum, Azotobacter, Bacillus, Ciavibacter, Enterobacter, Erwinia, Flavobacter, Klebsielia, Pseudomonas, Rhizobium, Serratia, Streptomyces and Xanthomonas. Symbiotic fungi, such as Trichoderma and Gliocladium are also possible hosts for expression of the inventive nucleotide sequences for the same purpose.

Techniques for these genetic manipulations are specific for the different available hosts and are known in the art. For example, the expression vectors pKK223-3 and pKK223-2 can be used to express heterologous genes in E. coli, either in transcriptional or translational fusion, behind the tac or trc promoter. For the expression of operons encoding multiple ORFS, the simplest procedure is to insert the operon into a vector such as pKK2233 in transcriptional fusion, allowing the cognate ribosome binding site of the heterologous genes to be used. Techniques for overexpression in gram-positive species such as Bacillus are also known in the art and can be used in the context of this invention (Quax et al. In.: Industrial Microorganisms: Basic and Applied Molecular Genetics, Eds. Baltz et al., American Society for Microbiology, Washington (1993)). Alternate systems for overexpression rely for example, on yeast vectors and include the use of Pichia, Saccharomyces and Kluyveromyces (Sreekrishna, In: industrial microorganisms: basic and applied molecular genetics, Baltz, Hegeman, and Skatrud eds., American Society for Microbiology, Washington (1993); Dequin & Barre, Biotechnology 12:173-177 (1994); van den Berg et al., Biotechnology 8:135-139 (1990)).

Expression of the Nucleotide Sequences in Plant Tissue

In a particularly preferred embodiment, at least one of the insecticidal toxins of the invention is expressed in a higher organism, e.g., a plant. In this case, transgenic plants expressing effective amounts of the toxins protect themselves from insect pests. When the insect starts feeding on such a transgenic plant, it also ingests the expressed toxins. This will deter the insect from further biting into the plant tissue or may even harm or kill the insect. A nucleotide sequence of the present invention is inserted into an expression cassette, which is then preferably stably integrated in the genome of said plant, In another preferred embodiment, the nucleotide sequence is included in a non-pathogenic self-replicating virus. Plants transformed in accordance with the present invention may be monocots or dicots and include, but are not limited to, maize, wheat, barley, rye, sweet potato, bean, pea, chicory, lettuce, cabbage, cauliflower, broccoli, turnip, radish, spinach, asparagus, onion, garlic, pepper, celery, squash, pumpkin, hemp, zucchini, apple, pear, quince, melon, plum, cherry, peach, nectarine, apricot, strawberry, grape, raspberry, blackberry, pineapple, avocado, papaya, mango, banana, soybean, tomato, sorghum, sugarcane, sugarbeet, sunflower, rapeseed, clover, tobacco, carrot, cotton, alfalfa, rice, potato, eggplant, cucumber, Arabidopsis, and woody plants such as coniferous and deciduous trees.

Once a desired nucleotide sequence has been transformed into a particular plant species, it may be propagated in that species or moved into other varieties of the same species, particularly including commercial varieties, using traditional breeding techniques.

A nucleotide sequence of this invention is preferably expressed in transgenic plants, thus causing the biosynthesis of the corresponding toxin in the transgenic plants. In this way, transgenic plants with enhanced resistance to insects are generated. For their expression in transgenic plants, the nucleotide sequences of the invention may require modification and optimization. Although in many cases genes from microbial organisms can be expressed in plants at high levels without modification, low expression in transgenic plants may result from microbial nucleotide sequences having codons that are not preferred in plants. It is known in the art that all organisms have specific preferences for codon usage, and the codons of the nucleotide sequences described in this invention can be changed to conform with plant preferences, while maintaining the amino acids encoded thereby. Furthermore, high expression in plants is best achieved from coding sequences that have at least about 35% GC content, preferably more than about 45%, more preferably more than about 50%, and most preferably more than about 60%. Microbial nucleotide sequences which have low GC contents may express poorly in plants due to the existence of ATTTA motifs which may destabilize messages, and AATAAA motifs which may cause inappropriate polyadenylation. Although preferred gene sequences may be adequately expressed in both monocotyledonous and dicotyledonous plant species, sequences can be modified to account for the specific codon preferences and GC content preferences of monocotyledons or dicotyledons as these preferences have been shown to differ (Murray et al. Nucl. Acids Res. 17: 477-498 (1989)). In addition, the nucleotide sequences are screened for the existence of illegitimate splice sites that may cause message truncation. All changes required to be made within the nucleotide sequences such as those described above are made using well known techniques of site directed mutagenesis, PCR, and synthetic gene construction.

For efficient initiation of translation, sequences adjacent to the initiating methionine may require modification. For example, they can be modified by the inclusion of sequences known to be effective in plants. Joshi has suggested an appropriate consensus for plants (NAR 15: 6643-6653 (1987)) and Clontech suggests a further consensus translation initiator (1993/1994 catalog, page 210). These consensuses are suitable for use with the nucleotide sequences of this invention. The sequences are incorporated into constructions comprising the nucleotide sequences, up to and including the ATG (whilst leaving the second amino acid unmodified), or alternatively up to and including the GTC subsequent to the ATG (with the possibility of modifying the second amino acid of the transgene).

Expression of the nucleotide sequences in transgenic plants is driven by promoters shown to be functional in plants. The choice of promoter will vary depending on the temporal and spatial requirements for expression, and also depending on the target species. Thus, expression of the nucleotide sequences of this invention in leaves, in ears, in inflorescences (e.g. spikes, panicles, cobs, etc.), in roots, and/or seedlings is preferred. In many cases, however, protection against more than one type of insect pest is sought, and thus expression in multiple tissues is desirable. Although many promoters from dicotyledons have been shown to be operational in monocotyledons and vice versa, ideally dicotyledonous promoters are selected for expression in dicotyledons, and monocotyledonous promoters for expression in monocotyledons. However, there is no restriction to the provenance of selected promoters; it is sufficient that they are operational in driving the expression of the nucleotide sequences in the desired cell.

Preferred promoters that are expressed constitutively include promoters from genes encoding actin or ubiquitin and the CAMV 35S and 19S promoters. The nucleotide sequences of this invention can also be expressed under the regulation of promoters that are chemically regulated. This enables the insecticidal toxins to be synthesized only when the crop plants are treated with the inducing chemicals.

A preferred category of promoters is that which is wound inducible. Numerous promoters have been described which are expressed at wound sites and also at the sites of phytopathogen infection. Ideally, such a promoter should only be active locally at the sites of infection, and in this way the insecticidal toxins only accumulate in cells which need to synthesize the insecticidal toxins to kill the invading insect pest. Preferred promoters of this kind include those described by Stanford et al. Mol. Gen. Genet. 215: 200-208 (1989), Xu et al. Plant Molec. Biol. 22: 573-588 (1993), Logemann et al. Plant Cell 1: 151-158 (1989), Rohrmeier & Lehle, Plant Molec. Biol. 22: 783-792 (1993), Firek et al. Plant Molec. Biol. 22: 129-142 (1993), and Warner et al. Plant J. 3: 191-201 (1993).

Especially preferred embodiments of the invention are transgenic plants expressing at least one of the nucleotide sequences of the invention in a root-preferred or root-specific fashion. Further preferred embodiments are transgenic plants expressing the nucleotide sequences in a wound-inducible or pathogen infection-inducible manner.

In addition to the selection of a suitable promoter, constructions for expression of an insecticidal toxin in plants require an appropriate transcription terminator to be attached downstream of the heterologous nucleotide sequence. Several such terminators are available and known in the art (e.g. tml from Agrobacterium, E9 from rbcs). Any available terminator known to function in plants can be used in the context of this invention.

Numerous other sequences can be incorporated into expression cassettes described in this invention. These include sequences which have been shown to enhance expression such as intron sequences (e.g. from Adhl and bronzel) and viral leader sequences (e.g. from TMV, MCMV and AMV).

It may be preferable to target expression of the nucleotide sequences of the present invention to different cellular localizations in the plant. In some cases, localization in the cytosol may be desirable, whereas in other cases, localization in some subcellular organelle may be preferred. Subcellular localization of transgene encoded enzymes is undertaken using techniques well known in the art Typically, the DNA encoding the target peptide from a known organelle-targeted gene product is manipulated and fused upstream of the nucleotide sequence. Many such target sequences are known for the chloroplast and their functioning in heterologous constructions has been shown. The expression of the nucleotide sequences of the present invention is also targeted to the endoplasmic reticulum or to the vacuoles of the host cells. Techniques to achieve this are well-known in the art.

Vectors suitable for plant transformation are described elsewhere in this specification. For Agrobacterium-mediated transformation, binary vectors or vectors carrying at least one T-DNA border sequence are suitable, whereas for direct gene transfer any vector is suitable and linear DNA containing only the construction of interest may be preferred. In the case of direct gene transfer, transformation with a single DNA species or co-transformation can be used (Schocher et al. Biotechnology 4: 1093-1096 (1986)). For both direct gene transfer and Agrobacterium-mediated transfer, transformation is usually (but not necessarily) undertaken with a selectable or screenable marker which may provide resistance to an antibiotic (kanamycin, hygromycin or methotrexate) or a herbicide (Basta). Examples of such markers are neomycin phosphotransferase, hygromycin phosphotransferase, dihydrofolate reductase, phosphinothricin acetyltransferase, 2,2-dichloroproprionic acid dehalogenase, acetohydroxyacid synthase, 5-enolpyruvyl-shikimate-phosphate synthase, haloarylnitrilase, protoporhyrinogen oxidase, acetyl-coenzyme A carboxylase, dihydropteroate synthase, chloramphenicol acetyl transferase, and •-glucuronidase. The choice of selectable or screenable marker for plant transformation is not, however, critical to the invention.

The recombinant DNA described above can be introduced into the plant cell in a number of art-recognized ways. Those skilled in the art will appreciate that the choice of method might depend on the type of plant targeted for transformation. Suitable methods of transforming plant cells include microinjection (Crossway et al., BioTechniques 4.,320-334 (1986)), electroporation (Riggs et al., Proc. Natl. Acad, Sci. USA 83.,5602-5606 (1986), Agrobacterium-mediated transformation (Hinchee et al., Biotechnology 6:915-921 (1988); See also, Ishida et al., Nature Biotechnology 14:745-750 (June 1996) (for maize transformation), direct gene transfer (Paszkowski et al., EMBO J. 3.2717-2722 (1984); Hayashimoto et al., Plant Physiol 93.857-863 (1990)(rice), and ballistic particle acceleration using devices available from Agracetus, Inc., Madison, Wis. and Dupont, Inc., Wilmington, Del. (see, for example, Sanford et al., U.S. Pat. No. 4,945,050; and McCabe et al., Biotechnology 6.923-926 (1988)). See also, Weissinger et al., Annual Rev Genet. 22.13 421-477 (1988); Sanford et al., Particulate Science and Technology 5.27-37 (1987)(onion); Svab et al., Proc. Natl. Acad. Sci. USA 87.—8526-8530 (1990) (tobacco chloroplast); Christou et al., Plant Physiol 87,671-674 (1988)(soybean); McCabe et al., BioTechnology 6.923-926 (1988)(soybean); Klein et al., Proc. Natl. Acad. Sci. USA, 85:4305-4309 (1988)(maize); Klein et al., BioTechnology 6.,559-563 (1988) (maize); Klein et al., Plant PhysioL 91.,440-444 (1988) (maize); Fromm et al., BioTechnology 8:833-839 (1990); and Gordon-Kamm et al., Plant Cell 2: 603-618 (1990) (maize); Koziel et al., Biotechnology 11: 194-200 (1993) (maize); Shimamoto et al., Nature 338: 274-277 (1989) (rice); Christou et al., Biotechnology 9: 957-962 (1991) (rice); Datta et al., BioTechnology 8.736-740 (1990) (rice); European Patent Application EP 0 332 581 (orchardgrass and other Pooideae); Vasil et al., Biotechnology 11: 1553-1558 (1993) (wheat); Weeks et al., Plant Physiol. 102:1077-1084 (1993) (wheat); Wan et al., Plant Physiol. 104:37-48 (1994) (barley); Jahne et al., Theor. Appl. Genet. 89:525-533 (1994)(barley); Umbeck et al., BioTechnology 5:263-266 (1987) (cotton); Casas et al., Proc. Natl. Acad. Sci. USA 90:11212-11216 (December 1993) (sorghum); Somers et al., BioTechnology 10:1 589-1594 (December 1992) (oat); Torbert et al., Plant Cell Reports 14:635-640 (1995) (oat); Weeks et al., Plant Physiol. 102:1077-1084 (1993) (wheat); Chang et al., WO 94/13822 (wheat) and Nehra et al., The Plant Journal 5:285-297 (1994) (wheat). A particularly preferred set of embodiments for the introduction of recombinant DNA molecules into maize by microprojectile bombardment can be found in Koziel et al., Biotechnology 11: 194-200(1993), Hill et al., Euphytica 85:119-123 (1995) and Koziel et al., Annals of the New York Academy of Sciences 792:164-171 (1996). An additional preferred embodiment is the protoplast transformation method for maize as disclosed in EP 0 292 435. Transformation of plants can be undertaken with a single DNA species or multiple DNA species (i.e. co-transformation).

In another preferred embodiment, a nucleotide sequence of the present invention is directly transformed into the plastid genome. A major advantage of plastid transformation is that plastids are generally capable of expressing bacterial genes without substantial modification, and plastids are capable of expressing multiple open reading frames under control of a single promoter. Plastid transformation technology is extensively described in U.S. Pat. Nos. 5,451,513, 5,545,817, and 5,545,818, in PCT application no. WO 95/16783, and in McBride et al. (1994) Proc. Natl. Acad. Sci. USA 91, 7301-7305. The basic technique for chloroplast transformation involves introducing regions of cloned plastid DNA flanking a selectable marker together with the gene of interest into a suitable target tissue, e.g., using biolistics or protoplast transformation (e.g., calcium chloride or PEG mediated transformation). The 1 to 1.5 kb flanking regions, termed targeting sequences, facilitate homologous recombination with the plastid genome and thus allow the replacement or modification of specific regions of the plastome. Initially, point mutations in the chloroplast 16S rRNA and rps12 genes conferring resistance to spectinomycin and/or streptomycin are utilized as selectable markers for transformation (Svab, Z., Hajdukiewicz, P., and Maliga, P. (1990) Proc. Natl. Acad. Sci. USA 87, 8526-8530; Staub, J. M., and Maliga, P. (1992) Plant Cell 4, 39-45). This resulted in stable homoplasmic transformants at a frequency of approximately one per 100 bombardments of target leaves. The presence of cloning sites between these markers allowed creation of a plastid targeting vector for introduction of foreign genes (Staub, J. M., and Maliga, P. (1993) EMBO J. 12, 601-606). Substantial increases in transformation frequency are obtained by replacement of the recessive RRNA or r-protein antibiotic resistance genes with a dominant selectable marker, the bacterial aada gene encoding the spectinomycin-detoxifying enzyme aminoglycoside-3′ adenyltransferase (Svab, Z., and Maliga, P. (1993) Proc. Natl. Acad. Sci. USA 90, 913-917). Previously, this marker had been used successfully for high-frequency transformation of the plastid genome of the green alga Chlamydomonas reinhardtii (Goldschmidt-Clermont, M. (1991) Nucl. Acids Res. 19: 4083-4089). Other selectable markers useful for plastid transformation are known in the art and encompassed within the scope of the invention. Typically, approximately 15-20 cell division cycles following transformation are required to reach a homoplastidic state. Plastid expression, in which genes are inserted by homologous recombination into all of the several thousand copies of the circular plastid genome present in each plant cell, takes advantage of the enormous copy number advantage over nuclear-expressed genes to permit expression levels that can readily exceed 10% of the total soluble plant protein. In a preferred embodiment, a nucleotide sequence of the present invention is inserted into a plastid targeting vector and transformed into the plastid genome of a desired plant host. Plants homoplastic for plastid genomes containing a nucleotide sequence of the present invention are obtained, and are preferentially capable of high expression of the nucleotide sequence.

Provisional Patent Application Ser. No. US 60/191806 filed Mar. 24, 2000, is hereby incorporated by reference.

<160> NUMBER OF SEQ ID NOS: 8

<210> SEQ ID NO 1

<211> LENGTH: 7551

<212> TYPE: DNA

<213> ORGANISM: Photorhabdus luminescens

<400> SEQUENCE: 1

atgaacgagt ctgtaaaaga gatacctgat gtattaaaaa gccagtgtgg tt

#ttaattgt     60

ctgacagata ttagccacag ctcttttaat gaatttcgcc agcaagtatc tg

#agcacctc    120

tcctggtccg aaacacacga cttatatcat gatgcacaac aggcacaaaa gg

#ataatcgc    180

ctgtatgaag cgcgtattct caaacgcgcc aatccccaat tacaaaatgc gg

#tgcatctt    240

gccattctcg ctcccaatgc tgaactgata ggctataaca atcaatttag cg

#gtagagcc    300

agtcaatatg ttgcgccggg taccgtttct tccatgttct cccccgccgc tt

#atttgact    360

gaactttatc gtgaagcacg caatttacac gcaagtgact ccgtttatta tc

#tggatacc    420

cgccgcccag atctcaaatc aatggcgctc agtcagcaaa atatggatat ag

#aattatcc    480

acactctctt tgtccaatga gctgttattg gaaagcatta aaactgaatc ta

#aactggaa    540

aactatacta aagtgatgga aatgctctcc actttccgtc cttccggcgc aa

#cgccttat    600

catgatgctt atgaaaatgt gcgtgaagtt atccagctac aagatcctgg ac

#ttgagcaa    660

ctcaatgcat caccggcaat tgccgggttg atgcatcaag cctccctatt gg

#gtattaac    720

gcttcaatct cgcctgagct atttaatatt ctgacggagg agattaccga ag

#gtaatgct    780

gaggaacttt ataagaaaaa ttttggtaat atcgaaccgg cctcattggc ta

#tgccggaa    840

taccttaaac gttattataa tttaagcgat gaagaactta gtcagtttat tg

#gtaaagcc    900

agcaattttg gtcaacagga atatagtaat aaccaactta ttactccggt ag

#tcaacagc    960

agtgatggca cggttaaggt atatcggatc acccgcgaat atacaaccaa tg

#cttatcaa   1020

atggatgtgg agctatttcc cttcggtggt gagaattatc ggttagatta ta

#aattcaaa   1080

aatttttata atgcctctta tttatccatc aagttaaatg ataaaagaga ac

#ttgttcga   1140

actgaaggcg ctcctcaagt caatatagaa tactccgcaa atatcacatt aa

#ataccgct   1200

gatatcagtc aaccttttga aattggcctg acacgagtac ttccttccgg tt

#cttgggca   1260

tatgccgccg caaaatttac cgttgaagag tataaccaat actcttttct gc

#taaaactt   1320

aacaaggcta ttcgtctatc acgtgcgaca gaattgtcac ccacgattct gg

#aaggcatt   1380

gtgcgcagtg ttaatctaca actggatatc aacacagacg tattaggtaa ag

#tttttctg   1440

actaaatatt atatgcagcg ttatgctatt catgctgaaa ctgccctgat ac

#tatgcaac   1500

gcgcctattt cacaacgttc atatgataat caacctagcc aatttgatcg cc

#tgtttaat   1560

acgccattac tgaacggaca atatttttct accggcgatg aggagattga tt

#taaattca   1620

ggtagcaccg gcgattggcg aaaaaccata cttaagcgtg catttaatat tg

#atgatgtc   1680

tcgctcttcc gcctgcttaa aattaccgac catgataata aagatggaaa aa

#ttaaaaat   1740

aacctaaaga atctttccaa tttatatatt ggaaaattac tggcagatat tc

#atcaatta   1800

accattgatg aactggattt attactgatt gccgtaggtg aaggaaaaac ta

#atttatcc   1860

gctatcagtg ataagcaatt ggctaccctg atcagaaaac tcaatactat ta

#ccagctgg   1920

ctacatacac agaagtggag tgtattccag ctatttatca tgacctccac ca

#gctataac   1980

aaaacgctaa cgcctgaaat taagaatttg ctggataccg tctaccacgg tt

#tacaaggt   2040

tttgataaag acaaagcaga tttgctacat gtcatggcgc cctatattgc gg

#ccaccttg   2100

caattatcat cggaaaatgt cgcccactcg gtactccttt gggcagataa gt

#tacagccc   2160

ggcgacggcg caatgacagc agaaaaattc tgggactggt tgaatactaa gt

#atacgccg   2220

ggttcatcgg aagccgtaga aacgcaggaa catatcgttc agtattgtca gg

#ctctggca   2280

caattggaaa tggtttacca ttccaccggc atcaacgaaa acgccttccg tc

#tatttgtg   2340

acaaaaccag agatgtttgg cgctgcaact ggagcagcgc ccgcgcatga tg

#ccctttca   2400

ctgattatgc tgacacgttt tgcggattgg gtgaacgcac taggcgaaaa ag

#cgtcctcg   2460

gtgctagcgg catttgaagc taactcgtta acggcagaac aactggctga tg

#ccatgaat   2520

cttgatgcta atttgctgtt gcaagccagt attcaagcac aaaatcatca ac

#atcttccc   2580

ccagtaactc cagaaaatgc gttctcctgt tggacatcta tcaatactat cc

#tgcaatgg   2640

gttaatgtcg cacaacaatt gaatgtcgcc ccacagggcg tttccgcttt gg

#tcgggctg   2700

gattatattc aatcaatgaa agagacaccg acctatgccc agtgggaaaa cg

#cggcaggc   2760

gtattaaccg ccgggttgaa ttcacaacag gctaatacat tacacgcttt tc

#tggatgaa   2820

tctcgcagtg ccgcattaag cacctactat atccgtcaag tcgccaaggc ag

#cggcggct   2880

attaaaagcc gtgatgactt gtatcaatac ttactgattg ataatcaggt tt

#ctgcggca   2940

ataaaaacca cccggatcgc cgaagccatt gccagtattc aactgtacgt ca

#accgggca   3000

ttggaaaatg tggaagaaaa tgccaattcg ggggttatca gccgccaatt ct

#ttatcgac   3060

tgggacaaat acaataaacg ctacagcact tgggcgggtg tttctcaatt ag

#tttactac   3120

ccggaaaact atattgatcc gaccatgcgt atcggacaaa ccaaaatgat gg

#acgcatta   3180

ctgcaatccg tcagccaaag ccaattaaac gccgataccg tcgaagatgc ct

#ttatgtct   3240

tatctgacat cgtttgaaca agtggctaat cttaaagtta ttagcgcata tc

#acgataat   3300

attaataacg atcaagggct gacctatttt atcggactca gtgaaactga tg

#ccggtgaa   3360

tattattggc gcagtgtcga tcacagtaaa ttcaacgacg gtaaattcgc gg

#ctaatgcc   3420

tggagtgaat ggcataaaat tgattgtcca attaaccctt ataaaagcac ta

#tccgtcca   3480

gtgatatata aatcccgcct gtatctgctc tggttggaac aaaaggagat ca

#ccaaacag   3540

acaggaaata gtaaagatgg ctatcaaact gaaacggatt atcgttatga ac

#taaaattg   3600

gcgcatatcc gctatgatgg cacttggaat acgccaatca cctttgatgt ca

#ataaaaaa   3660

atatccgagc taaaactgga aaaaaataga gcgcccggac tctattgtgc cg

#gttatcaa   3720

ggtgaagata cgttgctggt gatgttttat aaccaacaag acacactaga ta

#gttataaa   3780

aacgcttcaa tgcaaggact atatatcttt gctgatatgg catccaaaga ta

#tgacccca   3840

gaacagagca atgtttatcg ggataatagc tatcaacaat ttgataccaa ta

#atgtcaga   3900

agagtgaata accgctatgc agaggattat gagattcctt cctcggtaag ta

#gccgtaaa   3960

gactatggtt ggggagatta ttacctcagc atggtatata acggagatat tc

#caactatc   4020

aattacaaag ccgcatcaag tgatttaaaa atctatatct caccaaaatt aa

#gaattatt   4080

cataatggat atgaaggaca gaagcgcaat caatgcaatc tgatgaataa at

#atggcaaa   4140

ctaggtgata aatttattgt ttatactagc ttgggggtca atccaaataa ct

#cgtcaaat   4200

aagctcatgt tttaccccgt ctatcaatat agcggaaaca ccagtggact ca

#atcaaggg   4260

agactactat tccaccgtga caccacttat ccatctaaag tagaagcttg ga

#ttcctgga   4320

gcaaaacgtt ctctaaccaa ccaaaatgcc gccattggtg atgattatgc ta

#cagactct   4380

ctgaataaac cggatgatct taagcaatat atctttatga ctgacagtaa ag

#ggactgct   4440

actgatgtct caggcccagt agagattaat actgcaattt ctccagcaaa ag

#ttcagata   4500

atagtcaaag cgggtggcaa ggagcaaact tttaccgcag ataaagatgt ct

#ccattcag   4560

ccatcaccta gctttgatga aatgaattat caatttaatg cccttgaaat ag

#acggttct   4620

ggtctgaatt ttattaacaa ctcagccagt attgatgtta cttttaccgc at

#ttgcggag   4680

gatggccgca aactgggtta tgaaagtttc agtattcctg ttaccctcaa gg

#taagtacc   4740

gataatgccc tgaccctgca ccataatgaa aatggtgcgc aatatatgca at

#ggcaatcc   4800

tatcgtaccc gcctgaatac tctatttgcc cgccagttgg ttgcacgcgc ca

#ccaccgga   4860

atcgatacaa ttctgagtat ggaaactcag aatattcagg aaccgcagtt ag

#gcaaaggt   4920

ttctatgcta cgttcgtgat acctccctat aacctatcaa ctcatggtga tg

#aacgttgg   4980

tttaagcttt atatcaaaca tgttgttgat aataattcac atattatcta tt

#caggccag   5040

ctaacagata caaatataaa catcacatta tttattcctc ttgatgatgt cc

#cattgaat   5100

caagattatc acgccaaggt ttatatgacc ttcaagaaat caccatcaga tg

#gtacctgg   5160

tggggccctc actttgttag agatgataaa ggaatagtaa caataaaccc ta

#aatccatt   5220

ttgacccatt ttgagagcgt caatgtcctg aataatatta gtagcgaacc aa

#tggatttc   5280

agcggcgcta acagcctcta tttctgggaa ctgttctact ataccccgat gc

#tggttgct   5340

caacgtttgc tgcatgaaca gaacttcgat gaagccaacc gttggctgaa at

#atgtctgg   5400

agtccatccg gttatattgt ccacggccag attcagaact accagtggaa cg

#tccgcccg   5460

ttactggaag acaccagttg gaacagtgat cctttggatt ccgtcgatcc tg

#acgcggta   5520

gcacagcacg atccaatgca ctacaaagtt tcaactttta tgcgtacctt gg

#atctattg   5580

atagcacgcg gcgaccatgc ttatcgccaa ctggaacgag atacactcaa cg

#aagcgaag   5640

atgtggtata tgcaagcgct gcatctatta ggtgacaaac cttatctacc gc

#tgagtacg   5700

acatggagtg atccacgact agacagagcc gcggatatca ctacccaaaa tg

#ctcacgac   5760

agcgcaatag tcgctctgcg gcagaatata cctacaccgg cacctttatc at

#tgcgcagc   5820

gctaataccc tgactgatct cttcctgccg caaatcaatg aagtgatgat ga

#attactgg   5880

cagacattag ctcagagagt atacaatctg cgtcataacc tctctatcga cg

#gccagccg   5940

ttatatctgc caatctatgc cacaccggcc gatccgaaag cgttactcag cg

#ccgccgtt   6000

gccacttctc aaggtggagg caagctaccg gaatcattta tgtccctgtg gc

#gtttcccg   6060

cacatgctgg aaaatgcgcg cggcatggtt agccagctca cccagttcgg ct

#ccacgtta   6120

caaaatatta tcgaacgtca ggacgcggaa gcgctcaatg cgttattaca aa

#atcaggcc   6180

gccgagctga tattgactaa cctgagcatt caggacaaaa ccattgaaga at

#tggatgcc   6240

gagaaaacgg tgttggaaaa atccaaagcg ggagcacaat cgcgctttga ta

#gctacggc   6300

aaactgtacg atgagaatat caacgccggt gaaaaccaag ccatgacgct ac

#gagcgtcc   6360

gccgccgggc ttaccacggc agttcaggca tcccgtctgg ccggtgcggc gg

#ctgatctg   6420

gtgcctaaca tcttcggctt tgccggtggc ggcagccgtt ggggggctat cg

#ctgaggcg   6480

acaggttatg tgatggaatt ctccgcgaat gttatgaaca ccgaagcgga ta

#aaattagc   6540

caatctgaaa cctaccgtcg tcgccgtcag gagtgggaga tccagcggaa ta

#atgccgaa   6600

gcggaattga agcaaatcga tgctcagctc aaatcactcg ctgtacgccg cg

#aagccgcc   6660

gtattgcaga aaaccagtct gaaaacccaa caagaacaga cccaatctca at

#tggccttc   6720

ctgcaacgta agttcagcaa tcaggcgtta tacaactggc tgcgtggtcg ac

#tggcggcg   6780

atttacttcc agttctacga tttggccgtc gcgcgttgcc tgatggcaga ac

#aagcttac   6840

cgttgggaac tcaatgatga ctctgcccgc ttcattaaac cgggcgcctg gc

#agggaacc   6900

tatgccggtc tgcttgcagg tgaaaccttg atgctgagtc tggcacaaat gg

#aagacgct   6960

catctgaaac gcgataaacg cgcattagag gttgaacgca cagtatcgct gg

#ccgaagtt   7020

tatgcaggat taccaaaaga taacggtcca ttttccctgg ctcaggaaat tg

#acaagctg   7080

gtgagtcaag gttcaggcag tgccggcagt ggtaataata atttggcgtt cg

#gcgccggc   7140

acggacacta aaacctcttt gcaggcatca gtttcattcg ctgatttgaa aa

#ttcgtgaa   7200

gattacccgg catcgcttgg caaaattcga cgtatcaaac agatcagcgt ca

#ctttgccc   7260

gcgctactgg gaccgtatca ggatgtacag gcaatattgt cttacggcga ta

#aagccgga   7320

ttagctaacg gctgtgaagc gctggcagtt tctcacggta tgaatgacag cg

#gccaattc   7380

cagctcgatt tcaacgatgg caaattcctg ccattcgaag gcatcgccat tg

#atcaaggc   7440

acgctgacac tgagcttccc aaatgcatct atgccggaga aaggtaaaca ag

#ccactatg   7500

ttaaaaaccc tgaacgatat cattttgcat attcgctaca ccattaaata a

#           7551

<210> SEQ ID NO 2

<211> LENGTH: 2516

<212> TYPE: PRT

<213> ORGANISM: Photorhabdus luminescens

<400> SEQUENCE: 2

Met Asn Glu Ser Val Lys Glu Ile Pro Asp Va

#l Leu Lys Ser Gln Cys

1               5

#                10

#                15

Gly Phe Asn Cys Leu Thr Asp Ile Ser His Se

#r Ser Phe Asn Glu Phe

20

#            25

#            30

Arg Gln Gln Val Ser Glu His Leu Ser Trp Se

#r Glu Thr His Asp Leu

35

#        40

#        45

Tyr His Asp Ala Gln Gln Ala Gln Lys Asp As

#n Arg Leu Tyr Glu Ala

50

#    55

#    60

Arg Ile Leu Lys Arg Ala Asn Pro Gln Leu Gl

#n Asn Ala Val His Leu

65

#70

#75

#80

Ala Ile Leu Ala Pro Asn Ala Glu Leu Ile Gl

#y Tyr Asn Asn Gln Phe

85

#                90

#                95

Ser Gly Arg Ala Ser Gln Tyr Val Ala Pro Gl

#y Thr Val Ser Ser Met

100

#           105

#           110

Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Ty

#r Arg Glu Ala Arg Asn

115

#       120

#       125

Leu His Ala Ser Asp Ser Val Tyr Tyr Leu As

#p Thr Arg Arg Pro Asp

130

#   135

#   140

Leu Lys Ser Met Ala Leu Ser Gln Gln Asn Me

#t Asp Ile Glu Leu Ser

145                 1

#50                 1

#55                 1

#60

Thr Leu Ser Leu Ser Asn Glu Leu Leu Leu Gl

#u Ser Ile Lys Thr Glu

165

#               170

#               175

Ser Lys Leu Glu Asn Tyr Thr Lys Val Met Gl

#u Met Leu Ser Thr Phe

180

#           185

#           190

Arg Pro Ser Gly Ala Thr Pro Tyr His Asp Al

#a Tyr Glu Asn Val Arg

195

#       200

#       205

Glu Val Ile Gln Leu Gln Asp Pro Gly Leu Gl

#u Gln Leu Asn Ala Ser

210

#   215

#   220

Pro Ala Ile Ala Gly Leu Met His Gln Ala Se

#r Leu Leu Gly Ile Asn

225                 2

#30                 2

#35                 2

#40

Ala Ser Ile Ser Pro Glu Leu Phe Asn Ile Le

#u Thr Glu Glu Ile Thr

245

#               250

#               255

Glu Gly Asn Ala Glu Glu Leu Tyr Lys Lys As

#n Phe Gly Asn Ile Glu

260

#           265

#           270

Pro Ala Ser Leu Ala Met Pro Glu Tyr Leu Ly

#s Arg Tyr Tyr Asn Leu

275

#       280

#       285

Ser Asp Glu Glu Leu Ser Gln Phe Ile Gly Ly

#s Ala Ser Asn Phe Gly

290

#   295

#   300

Gln Gln Glu Tyr Ser Asn Asn Gln Leu Ile Th

#r Pro Val Val Asn Ser

305                 3

#10                 3

#15                 3

#20

Ser Asp Gly Thr Val Lys Val Tyr Arg Ile Th

#r Arg Glu Tyr Thr Thr

325

#               330

#               335

Asn Ala Tyr Gln Met Asp Val Glu Leu Phe Pr

#o Phe Gly Gly Glu Asn

340

#           345

#           350

Tyr Arg Leu Asp Tyr Lys Phe Lys Asn Phe Ty

#r Asn Ala Ser Tyr Leu

355

#       360

#       365

Ser Ile Lys Leu Asn Asp Lys Arg Glu Leu Va

#l Arg Thr Glu Gly Ala

370

#   375

#   380

Pro Gln Val Asn Ile Glu Tyr Ser Ala Asn Il

#e Thr Leu Asn Thr Ala

385                 3

#90                 3

#95                 4

#00

Asp Ile Ser Gln Pro Phe Glu Ile Gly Leu Th

#r Arg Val Leu Pro Ser

405

#               410

#               415

Gly Ser Trp Ala Tyr Ala Ala Ala Lys Phe Th

#r Val Glu Glu Tyr Asn

420

#           425

#           430

Gln Tyr Ser Phe Leu Leu Lys Leu Asn Lys Al

#a Ile Arg Leu Ser Arg

435

#       440

#       445

Ala Thr Glu Leu Ser Pro Thr Ile Leu Glu Gl

#y Ile Val Arg Ser Val

450

#   455

#   460

Asn Leu Gln Leu Asp Ile Asn Thr Asp Val Le

#u Gly Lys Val Phe Leu

465                 4

#70                 4

#75                 4

#80

Thr Lys Tyr Tyr Met Gln Arg Tyr Ala Ile Hi

#s Ala Glu Thr Ala Leu

485

#               490

#               495

Ile Leu Cys Asn Ala Pro Ile Ser Gln Arg Se

#r Tyr Asp Asn Gln Pro

500

#           505

#           510

Ser Gln Phe Asp Arg Leu Phe Asn Thr Pro Le

#u Leu Asn Gly Gln Tyr

515

#       520

#       525

Phe Ser Thr Gly Asp Glu Glu Ile Asp Leu As

#n Ser Gly Ser Thr Gly

530

#   535

#   540

Asp Trp Arg Lys Thr Ile Leu Lys Arg Ala Ph

#e Asn Ile Asp Asp Val

545                 5

#50                 5

#55                 5

#60

Ser Leu Phe Arg Leu Leu Lys Ile Thr Asp Hi

#s Asp Asn Lys Asp Gly

565

#               570

#               575

Lys Ile Lys Asn Asn Leu Lys Asn Leu Ser As

#n Leu Tyr Ile Gly Lys

580

#           585

#           590

Leu Leu Ala Asp Ile His Gln Leu Thr Ile As

#p Glu Leu Asp Leu Leu

595

#       600

#       605

Leu Ile Ala Val Gly Glu Gly Lys Thr Asn Le

#u Ser Ala Ile Ser Asp

610

#   615

#   620

Lys Gln Leu Ala Thr Leu Ile Arg Lys Leu As

#n Thr Ile Thr Ser Trp

625                 6

#30                 6

#35                 6

#40

Leu His Thr Gln Lys Trp Ser Val Phe Gln Le

#u Phe Ile Met Thr Ser

645

#               650

#               655

Thr Ser Tyr Asn Lys Thr Leu Thr Pro Glu Il

#e Lys Asn Leu Leu Asp

660

#           665

#           670

Thr Val Tyr His Gly Leu Gln Gly Phe Asp Ly

#s Asp Lys Ala Asp Leu

675

#       680

#       685

Leu His Val Met Ala Pro Tyr Ile Ala Ala Th

#r Leu Gln Leu Ser Ser

690

#   695

#   700

Glu Asn Val Ala His Ser Val Leu Leu Trp Al

#a Asp Lys Leu Gln Pro

705                 7

#10                 7

#15                 7

#20

Gly Asp Gly Ala Met Thr Ala Glu Lys Phe Tr

#p Asp Trp Leu Asn Thr

725

#               730

#               735

Lys Tyr Thr Pro Gly Ser Ser Glu Ala Val Gl

#u Thr Gln Glu His Ile

740

#           745

#           750

Val Gln Tyr Cys Gln Ala Leu Ala Gln Leu Gl

#u Met Val Tyr His Ser

755

#       760

#       765

Thr Gly Ile Asn Glu Asn Ala Phe Arg Leu Ph

#e Val Thr Lys Pro Glu

770

#   775

#   780

Met Phe Gly Ala Ala Thr Gly Ala Ala Pro Al

#a His Asp Ala Leu Ser

785                 7

#90                 7

#95                 8

#00

Leu Ile Met Leu Thr Arg Phe Ala Asp Trp Va

#l Asn Ala Leu Gly Glu

805

#               810

#               815

Lys Ala Ser Ser Val Leu Ala Ala Phe Glu Al

#a Asn Ser Leu Thr Ala

820

#           825

#           830

Glu Gln Leu Ala Asp Ala Met Asn Leu Asp Al

#a Asn Leu Leu Leu Gln

835

#       840

#       845

Ala Ser Ile Gln Ala Gln Asn His Gln His Le

#u Pro Pro Val Thr Pro

850

#   855

#   860

Glu Asn Ala Phe Ser Cys Trp Thr Ser Ile As

#n Thr Ile Leu Gln Trp

865                 8

#70                 8

#75                 8

#80

Val Asn Val Ala Gln Gln Leu Asn Val Ala Pr

#o Gln Gly Val Ser Ala

885

#               890

#               895

Leu Val Gly Leu Asp Tyr Ile Gln Ser Met Ly

#s Glu Thr Pro Thr Tyr

900

#           905

#           910

Ala Gln Trp Glu Asn Ala Ala Gly Val Leu Th

#r Ala Gly Leu Asn Ser

915

#       920

#       925

Gln Gln Ala Asn Thr Leu His Ala Phe Leu As

#p Glu Ser Arg Ser Ala

930

#   935

#   940

Ala Leu Ser Thr Tyr Tyr Ile Arg Gln Val Al

#a Lys Ala Ala Ala Ala

945                 9

#50                 9

#55                 9

#60

Ile Lys Ser Arg Asp Asp Leu Tyr Gln Tyr Le

#u Leu Ile Asp Asn Gln

965

#               970

#               975

Val Ser Ala Ala Ile Lys Thr Thr Arg Ile Al

#a Glu Ala Ile Ala Ser

980

#           985

#           990

Ile Gln Leu Tyr Val Asn Arg Ala  Leu Glu

#Asn Val Glu  Glu Asn Ala

995

#       1000

#       1005

Asn Ser  Gly Val Ile Ser Arg  Gln Phe P

#he Ile Asp  Trp Asp Lys

1010

#    1015

#    1020

Tyr Asn  Lys Arg Tyr Ser Thr  Trp Ala G

#ly Val Ser  Gln Leu Val

1025

#    1030

#    1035

Tyr Tyr  Pro Glu Asn Tyr Ile  Asp Pro T

#hr Met Arg  Ile Gly Gln

1040

#    1045

#    1050

Thr Lys  Met Met Asp Ala Leu  Leu Gln S

#er Val Ser  Gln Ser Gln

1055

#    1060

#    1065

Leu Asn  Ala Asp Thr Val Glu  Asp Ala P

#he Met Ser  Tyr Leu Thr

1070

#    1075

#    1080

Ser Phe  Glu Gln Val Ala Asn  Leu Lys V

#al Ile Ser  Ala Tyr His

1085

#    1090

#    1095

Asp Asn  Ile Asn Asn Asp Gln  Gly Leu T

#hr Tyr Phe  Ile Gly Leu

1100

#    1105

#    1110

Ser Glu  Thr Asp Ala Gly Glu  Tyr Tyr T

#rp Arg Ser  Val Asp His

1115

#    1120

#    1125

Ser Lys  Phe Asn Asp Gly Lys  Phe Ala A

#la Asn Ala  Trp Ser Glu

1130

#    1135

#    1140

Trp His  Lys Ile Asp Cys Pro  Ile Asn P

#ro Tyr Lys  Ser Thr Ile

1145

#    1150

#    1155

Arg Pro  Val Ile Tyr Lys Ser  Arg Leu T

#yr Leu Leu  Trp Leu Glu

1160

#    1165

#    1170

Gln Lys  Glu Ile Thr Lys Gln  Thr Gly A

#sn Ser Lys  Asp Gly Tyr

1175

#    1180

#    1185

Gln Thr  Glu Thr Asp Tyr Arg  Tyr Glu L

#eu Lys Leu  Ala His Ile

1190

#    1195

#    1200

Arg Tyr  Asp Gly Thr Trp Asn  Thr Pro I

#le Thr Phe  Asp Val Asn

1205

#    1210

#    1215

Lys Lys  Ile Ser Glu Leu Lys  Leu Glu L

#ys Asn Arg  Ala Pro Gly

1220

#    1225

#    1230

Leu Tyr  Cys Ala Gly Tyr Gln  Gly Glu A

#sp Thr Leu  Leu Val Met

1235

#    1240

#    1245

Phe Tyr  Asn Gln Gln Asp Thr  Leu Asp S

#er Tyr Lys  Asn Ala Ser

1250

#    1255

#    1260

Met Gln  Gly Leu Tyr Ile Phe  Ala Asp M

#et Ala Ser  Lys Asp Met

1265

#    1270

#    1275

Thr Pro  Glu Gln Ser Asn Val  Tyr Arg A

#sp Asn Ser  Tyr Gln Gln

1280

#    1285

#    1290

Phe Asp  Thr Asn Asn Val Arg  Arg Val A

#sn Asn Arg  Tyr Ala Glu

1295

#    1300

#    1305

Asp Tyr  Glu Ile Pro Ser Ser  Val Ser S

#er Arg Lys  Asp Tyr Gly

1310

#    1315

#    1320

Trp Gly  Asp Tyr Tyr Leu Ser  Met Val T

#yr Asn Gly  Asp Ile Pro

1325

#    1330

#    1335

Thr Ile  Asn Tyr Lys Ala Ala  Ser Ser A

#sp Leu Lys  Ile Tyr Ile

1340

#    1345

#    1350

Ser Pro  Lys Leu Arg Ile Ile  His Asn G

#ly Tyr Glu  Gly Gln Lys

1355

#    1360

#    1365

Arg Asn  Gln Cys Asn Leu Met  Asn Lys T

#yr Gly Lys  Leu Gly Asp

1370

#    1375

#    1380

Lys Phe  Ile Val Tyr Thr Ser  Leu Gly V

#al Asn Pro  Asn Asn Ser

1385

#    1390

#    1395

Ser Asn  Lys Leu Met Phe Tyr  Pro Val T

#yr Gln Tyr  Ser Gly Asn

1400

#    1405

#    1410

Thr Ser  Gly Leu Asn Gln Gly  Arg Leu L

#eu Phe His  Arg Asp Thr

1415

#    1420

#    1425

Thr Tyr  Pro Ser Lys Val Glu  Ala Trp I

#le Pro Gly  Ala Lys Arg

1430

#    1435

#    1440

Ser Leu  Thr Asn Gln Asn Ala  Ala Ile G

#ly Asp Asp  Tyr Ala Thr

1445

#    1450

#    1455

Asp Ser  Leu Asn Lys Pro Asp  Asp Leu L

#ys Gln Tyr  Ile Phe Met

1460

#    1465

#    1470

Thr Asp  Ser Lys Gly Thr Ala  Thr Asp V

#al Ser Gly  Pro Val Glu

1475

#    1480

#    1485

Ile Asn  Thr Ala Ile Ser Pro  Ala Lys V

#al Gln Ile  Ile Val Lys

1490

#    1495

#    1500

Ala Gly  Gly Lys Glu Gln Thr  Phe Thr A

#la Asp Lys  Asp Val Ser

1505

#    1510

#    1515

Ile Gln  Pro Ser Pro Ser Phe  Asp Glu M

#et Asn Tyr  Gln Phe Asn

1520

#    1525

#    1530

Ala Leu  Glu Ile Asp Gly Ser  Gly Leu A

#sn Phe Ile  Asn Asn Ser

1535

#    1540

#    1545

Ala Ser  Ile Asp Val Thr Phe  Thr Ala P

#he Ala Glu  Asp Gly Arg

1550

#    1555

#    1560

Lys Leu  Gly Tyr Glu Ser Phe  Ser Ile P

#ro Val Thr  Leu Lys Val

1565

#    1570

#    1575

Ser Thr  Asp Asn Ala Leu Thr  Leu His H

#is Asn Glu  Asn Gly Ala

1580

#    1585

#    1590

Gln Tyr  Met Gln Trp Gln Ser  Tyr Arg T

#hr Arg Leu  Asn Thr Leu

1595

#    1600

#    1605

Phe Ala  Arg Gln Leu Val Ala  Arg Ala T

#hr Thr Gly  Ile Asp Thr

1610

#    1615

#    1620

Ile Leu  Ser Met Glu Thr Gln  Asn Ile G

#ln Glu Pro  Gln Leu Gly

1625

#    1630

#    1635

Lys Gly  Phe Tyr Ala Thr Phe  Val Ile P

#ro Pro Tyr  Asn Leu Ser

1640

#    1645

#    1650

Thr His  Gly Asp Glu Arg Trp  Phe Lys L

#eu Tyr Ile  Lys His Val

1655

#    1660

#    1665

Val Asp  Asn Asn Ser His Ile  Ile Tyr S

#er Gly Gln  Leu Thr Asp

1670

#    1675

#    1680

Thr Asn  Ile Asn Ile Thr Leu  Phe Ile P

#ro Leu Asp  Asp Val Pro

1685

#    1690

#    1695

Leu Asn  Gln Asp Tyr His Ala  Lys Val T

#yr Met Thr  Phe Lys Lys

1700

#    1705

#    1710

Ser Pro  Ser Asp Gly Thr Trp  Trp Gly P

#ro His Phe  Val Arg Asp

1715

#    1720

#    1725

Asp Lys  Gly Ile Val Thr Ile  Asn Pro L

#ys Ser Ile  Leu Thr His

1730

#    1735

#    1740

Phe Glu  Ser Val Asn Val Leu  Asn Asn I

#le Ser Ser  Glu Pro Met

1745

#    1750

#    1755

Asp Phe  Ser Gly Ala Asn Ser  Leu Tyr P

#he Trp Glu  Leu Phe Tyr

1760

#    1765

#    1770

Tyr Thr  Pro Met Leu Val Ala  Gln Arg L

#eu Leu His  Glu Gln Asn

1775

#    1780

#    1785

Phe Asp  Glu Ala Asn Arg Trp  Leu Lys T

#yr Val Trp  Ser Pro Ser

1790

#    1795

#    1800

Gly Tyr  Ile Val His Gly Gln  Ile Gln A

#sn Tyr Gln  Trp Asn Val

1805

#    1810

#    1815

Arg Pro  Leu Leu Glu Asp Thr  Ser Trp A

#sn Ser Asp  Pro Leu Asp

1820

#    1825

#    1830

Ser Val  Asp Pro Asp Ala Val  Ala Gln H

#is Asp Pro  Met His Tyr

1835

#    1840

#    1845

Lys Val  Ser Thr Phe Met Arg  Thr Leu A

#sp Leu Leu  Ile Ala Arg

1850

#    1855

#    1860

Gly Asp  His Ala Tyr Arg Gln  Leu Glu A

#rg Asp Thr  Leu Asn Glu

1865

#    1870

#    1875

Ala Lys  Met Trp Tyr Met Gln  Ala Leu H

#is Leu Leu  Gly Asp Lys

1880

#    1885

#    1890

Pro Tyr  Leu Pro Leu Ser Thr  Thr Trp S

#er Asp Pro  Arg Leu Asp

1895

#    1900

#    1905

Arg Ala  Ala Asp Ile Thr Thr  Gln Asn A

#la His Asp  Ser Ala Ile

1910

#    1915

#    1920

Val Ala  Leu Arg Gln Asn Ile  Pro Thr P

#ro Ala Pro  Leu Ser Leu

1925

#    1930

#    1935

Arg Ser  Ala Asn Thr Leu Thr  Asp Leu P

#he Leu Pro  Gln Ile Asn

1940

#    1945

#    1950

Glu Val  Met Met Asn Tyr Trp  Gln Thr L

#eu Ala Gln  Arg Val Tyr

1955

#    1960

#    1965

Asn Leu  Arg His Asn Leu Ser  Ile Asp G

#ly Gln Pro  Leu Tyr Leu

1970

#    1975

#    1980

Pro Ile  Tyr Ala Thr Pro Ala  Asp Pro L

#ys Ala Leu  Leu Ser Ala

1985

#    1990

#    1995

Ala Val  Ala Thr Ser Gln Gly  Gly Gly L

#ys Leu Pro  Glu Ser Phe

2000

#    2005

#    2010

Met Ser  Leu Trp Arg Phe Pro  His Met L

#eu Glu Asn  Ala Arg Gly

2015

#    2020

#    2025

Met Val  Ser Gln Leu Thr Gln  Phe Gly S

#er Thr Leu  Gln Asn Ile

2030

#    2035

#    2040

Ile Glu  Arg Gln Asp Ala Glu  Ala Leu A

#sn Ala Leu  Leu Gln Asn

2045

#    2050

#    2055

Gln Ala  Ala Glu Leu Ile Leu  Thr Asn L

#eu Ser Ile  Gln Asp Lys

2060

#    2065

#    2070

Thr Ile  Glu Glu Leu Asp Ala  Glu Lys T

#hr Val Leu  Glu Lys Ser

2075

#    2080

#    2085

Lys Ala  Gly Ala Gln Ser Arg  Phe Asp S

#er Tyr Gly  Lys Leu Tyr

2090

#    2095

#    2100

Asp Glu  Asn Ile Asn Ala Gly  Glu Asn G

#ln Ala Met  Thr Leu Arg

2105

#    2110

#    2115

Ala Ser  Ala Ala Gly Leu Thr  Thr Ala V

#al Gln Ala  Ser Arg Leu

2120

#    2125

#    2130

Ala Gly  Ala Ala Ala Asp Leu  Val Pro A

#sn Ile Phe  Gly Phe Ala

2135

#    2140

#    2145

Gly Gly  Gly Ser Arg Trp Gly  Ala Ile A

#la Glu Ala  Thr Gly Tyr

2150

#    2155

#    2160

Val Met  Glu Phe Ser Ala Asn  Val Met A

#sn Thr Glu  Ala Asp Lys

2165

#    2170

#    2175

Ile Ser  Gln Ser Glu Thr Tyr  Arg Arg A

#rg Arg Gln  Glu Trp Glu

2180

#    2185

#    2190

Ile Gln  Arg Asn Asn Ala Glu  Ala Glu L

#eu Lys Gln  Ile Asp Ala

2195

#    2200

#    2205

Gln Leu  Lys Ser Leu Ala Val  Arg Arg G

#lu Ala Ala  Val Leu Gln

2210

#    2215

#    2220

Lys Thr  Ser Leu Lys Thr Gln  Gln Glu G

#ln Thr Gln  Ser Gln Leu

2225

#    2230

#    2235

Ala Phe  Leu Gln Arg Lys Phe  Ser Asn G

#ln Ala Leu  Tyr Asn Trp

2240

#    2245

#    2250

Leu Arg  Gly Arg Leu Ala Ala  Ile Tyr P

#he Gln Phe  Tyr Asp Leu

2255

#    2260

#    2265

Ala Val  Ala Arg Cys Leu Met  Ala Glu G

#ln Ala Tyr  Arg Trp Glu

2270

#    2275

#    2280

Leu Asn  Asp Asp Ser Ala Arg  Phe Ile L

#ys Pro Gly  Ala Trp Gln

2285

#    2290

#    2295

Gly Thr  Tyr Ala Gly Leu Leu  Ala Gly G

#lu Thr Leu  Met Leu Ser

2300

#    2305

#    2310

Leu Ala  Gln Met Glu Asp Ala  His Leu L

#ys Arg Asp  Lys Arg Ala

2315

#    2320

#    2325

Leu Glu  Val Glu Arg Thr Val  Ser Leu A

#la Glu Val  Tyr Ala Gly

2330

#    2335

#    2340

Leu Pro  Lys Asp Asn Gly Pro  Phe Ser L

#eu Ala Gln  Glu Ile Asp

2345

#    2350

#    2355

Lys Leu  Val Ser Gln Gly Ser  Gly Ser A

#la Gly Ser  Gly Asn Asn

2360

#    2365

#    2370

Asn Leu  Ala Phe Gly Ala Gly  Thr Asp T

#hr Lys Thr  Ser Leu Gln

2375

#    2380

#    2385

Ala Ser  Val Ser Phe Ala Asp  Leu Lys I

#le Arg Glu  Asp Tyr Pro

2390

#    2395

#    2400

Ala Ser  Leu Gly Lys Ile Arg  Arg Ile L

#ys Gln Ile  Ser Val Thr

2405

#    2410

#    2415

Leu Pro  Ala Leu Leu Gly Pro  Tyr Gln A

#sp Val Gln  Ala Ile Leu

2420

#    2425

#    2430

Ser Tyr  Gly Asp Lys Ala Gly  Leu Ala A

#sn Gly Cys  Glu Ala Leu

2435

#    2440

#    2445

Ala Val  Ser His Gly Met Asn  Asp Ser G

#ly Gln Phe  Gln Leu Asp

2450

#    2455

#    2460

Phe Asn  Asp Gly Lys Phe Leu  Pro Phe G

#lu Gly Ile  Ala Ile Asp

2465

#    2470

#    2475

Gln Gly  Thr Leu Thr Leu Ser  Phe Pro A

#sn Ala Ser  Met Pro Glu

2480

#    2485

#    2490

Lys Gly  Lys Gln Ala Thr Met  Leu Lys T

#hr Leu Asn  Asp Ile Ile

2495

#    2500

#    2505

Leu His  Ile Arg Tyr Thr Ile  Lys

2510

#    2515

<210> SEQ ID NO 3

<211> LENGTH: 4431

<212> TYPE: DNA

<213> ORGANISM: Photorhabdus luminescens

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(4431)

<400> SEQUENCE: 3

atg cag aat tca caa aca ttc agt gtt acc ga

#g ctg tca tta ccc aaa       48

Met Gln Asn Ser Gln Thr Phe Ser Val Thr Gl

#u Leu Ser Leu Pro Lys

1               5

#                10

#                15

ggc ggc ggc gct att acc ggt atg ggt gaa gc

#a tta aca cca gcc ggg       96

Gly Gly Gly Ala Ile Thr Gly Met Gly Glu Al

#a Leu Thr Pro Ala Gly

20

#            25

#            30

ccg gat ggt atg gcc gcc tta tcc ctg cca tt

#a ccc att tcc gcc ggg      144

Pro Asp Gly Met Ala Ala Leu Ser Leu Pro Le

#u Pro Ile Ser Ala Gly

35

#        40

#        45

cgt ggt tac gca ccc tcg ctc act ctg aat ta

#c aac agt gga acc ggt      192

Arg Gly Tyr Ala Pro Ser Leu Thr Leu Asn Ty

#r Asn Ser Gly Thr Gly

50

#    55

#    60

aac agc cca ttt ggt ctc ggt tgg gac tgc gg

#c gtc atg gca att cgt      240

Asn Ser Pro Phe Gly Leu Gly Trp Asp Cys Gl

#y Val Met Ala Ile Arg

65

#70

#75

#80

cgt cgc acc agt acc ggc gta ccg aat tac ga

#t gaa acc gat act ttt      288

Arg Arg Thr Ser Thr Gly Val Pro Asn Tyr As

#p Glu Thr Asp Thr Phe

85

#                90

#                95

ctg ggg ccg gaa ggt gaa gtg ttg gtc gta gc

#a tta aat gag gca ggt      336

Leu Gly Pro Glu Gly Glu Val Leu Val Val Al

#a Leu Asn Glu Ala Gly

100

#           105

#           110

caa gct gat atc cgc agt gaa tcc tca ttg ca

#g ggc atc aat ttg ggt      384

Gln Ala Asp Ile Arg Ser Glu Ser Ser Leu Gl

#n Gly Ile Asn Leu Gly

115

#       120

#       125

gcg acc ttc acc gtt acc tgt tat cgc tcc cg

#c cta gaa agc cac ttt      432

Ala Thr Phe Thr Val Thr Cys Tyr Arg Ser Ar

#g Leu Glu Ser His Phe

130

#   135

#   140

aac cgg ttg gaa tac tgg caa ccc caa aca ac

#c ggc gca acc gat ttc      480

Asn Arg Leu Glu Tyr Trp Gln Pro Gln Thr Th

#r Gly Ala Thr Asp Phe

145                 1

#50                 1

#55                 1

#60

tgg ctg ata tac agc ccc gac gga cag gtc ca

#t tta ctg ggc aaa aat      528

Trp Leu Ile Tyr Ser Pro Asp Gly Gln Val Hi

#s Leu Leu Gly Lys Asn

165

#               170

#               175

cct cag gca cgt atc agc aat cca ctc aat gt

#t aac caa aca gcg caa      576

Pro Gln Ala Arg Ile Ser Asn Pro Leu Asn Va

#l Asn Gln Thr Ala Gln

180

#           185

#           190

tgg ctg ttg gaa gcc tcg ata tca tcc cac ag

#c gaa cag att tat tat      624

Trp Leu Leu Glu Ala Ser Ile Ser Ser His Se

#r Glu Gln Ile Tyr Tyr

195

#       200

#       205

caa tat cgc gct gaa gat gaa gca ggt tgt ga

#a acc gac gag cta gca      672

Gln Tyr Arg Ala Glu Asp Glu Ala Gly Cys Gl

#u Thr Asp Glu Leu Ala

210

#   215

#   220

gcc cac ccc agc gca acc gtt cag cgc tac ct

#g caa aca gta cat tac      720

Ala His Pro Ser Ala Thr Val Gln Arg Tyr Le

#u Gln Thr Val His Tyr

225                 2

#30                 2

#35                 2

#40

ggg aac ctg acc gcc agc gac gtt ttt cct ac

#a cta aac gga gat gac      768

Gly Asn Leu Thr Ala Ser Asp Val Phe Pro Th

#r Leu Asn Gly Asp Asp

245

#               250

#               255

cca ctt aaa tct ggc tgg atg ttc tgt tta gt

#a ttt gac tac ggt gag      816

Pro Leu Lys Ser Gly Trp Met Phe Cys Leu Va

#l Phe Asp Tyr Gly Glu

260

#           265

#           270

cgc aaa aac agc tta tct gaa atg ccg ctg tt

#t aaa gcc aca ggc aat      864

Arg Lys Asn Ser Leu Ser Glu Met Pro Leu Ph

#e Lys Ala Thr Gly Asn

275

#       280

#       285

tgg ctt tgc cga aaa gac cgt ttt tcc cgt ta

#t gag tac ggt ttt gaa      912

Trp Leu Cys Arg Lys Asp Arg Phe Ser Arg Ty

#r Glu Tyr Gly Phe Glu

290

#   295

#   300

ttg cgt act cgc cgc tta tgc cgc caa ata ct

#g atg ttt cac cgt cta      960

Leu Arg Thr Arg Arg Leu Cys Arg Gln Ile Le

#u Met Phe His Arg Leu

305                 3

#10                 3

#15                 3

#20

caa acc cta tct ggt cag gca aag ggg gat ga

#t gaa cct gcg cta gtg     1008

Gln Thr Leu Ser Gly Gln Ala Lys Gly Asp As

#p Glu Pro Ala Leu Val

325

#               330

#               335

tcg cgt ctg ata ctg gat tat gac gaa aac gc

#g atg gtc agt acg ctc     1056

Ser Arg Leu Ile Leu Asp Tyr Asp Glu Asn Al

#a Met Val Ser Thr Leu

340

#           345

#           350

gtt tct gtc cgc cgg gta ggc cat gag gac aa

#c aac acg gtt acc gcg     1104

Val Ser Val Arg Arg Val Gly His Glu Asp As

#n Asn Thr Val Thr Ala

355

#       360

#       365

ctg cca cca ctg gaa ctg gcc tat cag cct tt

#t gag cca gaa caa acc     1152

Leu Pro Pro Leu Glu Leu Ala Tyr Gln Pro Ph

#e Glu Pro Glu Gln Thr

370

#   375

#   380

gca ctc tgg caa tca atg gat gta ctg gca aa

#t ttc aac acc att cag     1200

Ala Leu Trp Gln Ser Met Asp Val Leu Ala As

#n Phe Asn Thr Ile Gln

385                 3

#90                 3

#95                 4

#00

cgc tgg caa ctg ctt gac ctg aaa gga gaa gg

#c gtg ccc ggc att ctc     1248

Arg Trp Gln Leu Leu Asp Leu Lys Gly Glu Gl

#y Val Pro Gly Ile Leu

405

#               410

#               415

tat cag gat aga aat ggc tgg tgg tat cga tc

#t gcc caa cgt cag gcc     1296

Tyr Gln Asp Arg Asn Gly Trp Trp Tyr Arg Se

#r Ala Gln Arg Gln Ala

420

#           425

#           430

ggg gaa gag atg aat gcg gtc acc tgg ggg aa

#a atg caa ctc ctt ccc     1344

Gly Glu Glu Met Asn Ala Val Thr Trp Gly Ly

#s Met Gln Leu Leu Pro

435

#       440

#       445

atc aca cca gct gtg cag gat aac gcc tca ct

#g atg gat att aac ggt     1392

Ile Thr Pro Ala Val Gln Asp Asn Ala Ser Le

#u Met Asp Ile Asn Gly

450

#   455

#   460

gac ggg caa ctg gac tgg gtg att acc ggg cc

#g ggg cta agg ggc tat     1440

Asp Gly Gln Leu Asp Trp Val Ile Thr Gly Pr

#o Gly Leu Arg Gly Tyr

465                 4

#70                 4

#75                 4

#80

cac agc caa cac ccg gat ggc agt tgg acg cg

#t ttt acg cca tta cat     1488

His Ser Gln His Pro Asp Gly Ser Trp Thr Ar

#g Phe Thr Pro Leu His

485

#               490

#               495

gcc ctg ccg ata gaa tat tct cat cct cgc gc

#t caa ctt gcc gat tta     1536

Ala Leu Pro Ile Glu Tyr Ser His Pro Arg Al

#a Gln Leu Ala Asp Leu

500

#           505

#           510

atg gga gcc ggg ctg tcc gat tta gtg cta at

#t ggt ccc aaa agt gtg     1584

Met Gly Ala Gly Leu Ser Asp Leu Val Leu Il

#e Gly Pro Lys Ser Val

515

#       520

#       525

cgc tta tat gtc aat aac cgt gat ggt ttt ac

#c gaa ggg cgg gat gtg     1632

Arg Leu Tyr Val Asn Asn Arg Asp Gly Phe Th

#r Glu Gly Arg Asp Val

530

#   535

#   540

gtg caa tcc ggt gat atc acc ctg ccg cta cc

#g ggc gcc gat gcc cgt     1680

Val Gln Ser Gly Asp Ile Thr Leu Pro Leu Pr

#o Gly Ala Asp Ala Arg

545                 5

#50                 5

#55                 5

#60

aag tta gtg gca ttt agt gac gta ctg ggt tc

#a ggc caa gca cat ctg     1728

Lys Leu Val Ala Phe Ser Asp Val Leu Gly Se

#r Gly Gln Ala His Leu

565

#               570

#               575

gtt gaa gtt agt gca act caa gtc acc tgc tg

#g ccg aat ctg ggg cat     1776

Val Glu Val Ser Ala Thr Gln Val Thr Cys Tr

#p Pro Asn Leu Gly His

580

#           585

#           590

ggc cgt ttt ggt cag cca atc gta ttg ccg gg

#a ttc agc caa tct gcc     1824

Gly Arg Phe Gly Gln Pro Ile Val Leu Pro Gl

#y Phe Ser Gln Ser Ala

595

#       600

#       605

gcc agt ttt aat cct gat cga gtt cat ctg gc

#c gat ttg gat ggg agc     1872

Ala Ser Phe Asn Pro Asp Arg Val His Leu Al

#a Asp Leu Asp Gly Ser

610

#   615

#   620

ggc cct gcc gat ttg att tat gtt cat gct ga

#c cgt ctg gat att ttc     1920

Gly Pro Ala Asp Leu Ile Tyr Val His Ala As

#p Arg Leu Asp Ile Phe

625                 6

#30                 6

#35                 6

#40

agc aat gaa agt ggc aac ggt ttt gca aaa cc

#a ttc aca ctc tct ttt     1968

Ser Asn Glu Ser Gly Asn Gly Phe Ala Lys Pr

#o Phe Thr Leu Ser Phe

645

#               650

#               655

cct gac ggc ctg cgt ttt gat gat acc tgc ca

#g ttg caa gta gcc gat     2016

Pro Asp Gly Leu Arg Phe Asp Asp Thr Cys Gl

#n Leu Gln Val Ala Asp

660

#           665

#           670

gta caa ggg tta ggc gtt gtc agc ctg atc ct

#a agc gta ccg cat atg     2064

Val Gln Gly Leu Gly Val Val Ser Leu Ile Le

#u Ser Val Pro His Met

675

#       680

#       685

gcg cca cat cat tgg cgc tgc gat ctg acc aa

#c gcg aaa ccg tgg tta     2112

Ala Pro His His Trp Arg Cys Asp Leu Thr As

#n Ala Lys Pro Trp Leu

690

#   695

#   700

ctc agt gaa acg aac aac aat atg ggg gcc aa

#t cac acc ttg cat tac     2160

Leu Ser Glu Thr Asn Asn Asn Met Gly Ala As

#n His Thr Leu His Tyr

705                 7

#10                 7

#15                 7

#20

cgt agc tct gtc cag ttc tgg ctg gat gaa aa

#a gct gcg gca ttg gct     2208

Arg Ser Ser Val Gln Phe Trp Leu Asp Glu Ly

#s Ala Ala Ala Leu Ala

725

#               730

#               735

acc gga caa aca ccg gtc tgt tac ctg ccc tt

#c ccg gtc cat acc ctt     2256

Thr Gly Gln Thr Pro Val Cys Tyr Leu Pro Ph

#e Pro Val His Thr Leu

740

#           745

#           750

tgg caa aca gaa acc gag gat gaa atc agc gg

#c aat aag tta gtg acc     2304

Trp Gln Thr Glu Thr Glu Asp Glu Ile Ser Gl

#y Asn Lys Leu Val Thr

755

#       760

#       765

acg tta cgt tat gct cac ggc gct tgg gat gg

#a cgt gaa cgg gaa ttt     2352

Thr Leu Arg Tyr Ala His Gly Ala Trp Asp Gl

#y Arg Glu Arg Glu Phe

770

#   775

#   780

cgt ggc ttt ggt tat gtt gag cag aca gac ag

#c cat caa ctc gct caa     2400

Arg Gly Phe Gly Tyr Val Glu Gln Thr Asp Se

#r His Gln Leu Ala Gln

785                 7

#90                 7

#95                 8

#00

ggc aat gcg ccg gaa cgt aca cca ccg gca ct

#c acc aaa agc tgg tat     2448

Gly Asn Ala Pro Glu Arg Thr Pro Pro Ala Le

#u Thr Lys Ser Trp Tyr

805

#               810

#               815

gcc acc gga tta cct gcg gta gat aat gcg tt

#a tcc gcc ggg tat tgg     2496

Ala Thr Gly Leu Pro Ala Val Asp Asn Ala Le

#u Ser Ala Gly Tyr Trp

820

#           825

#           830

cgt ggc gat aag caa gct ttc gcc ggt ttt ac

#g cca cgt ttt act ctc     2544

Arg Gly Asp Lys Gln Ala Phe Ala Gly Phe Th

#r Pro Arg Phe Thr Leu

835

#       840

#       845

tgg aaa gag ggc aaa gat gtt cca ctg aca cc

#g gaa gat gac cat aat     2592

Trp Lys Glu Gly Lys Asp Val Pro Leu Thr Pr

#o Glu Asp Asp His Asn

850

#   855

#   860

cta tac tgg tta aac cgg gcg cta aaa ggt ca

#g cca ctg cgt agt gaa     2640

Leu Tyr Trp Leu Asn Arg Ala Leu Lys Gly Gl

#n Pro Leu Arg Ser Glu

865                 8

#70                 8

#75                 8

#80

ctc tac ggg ctg gat ggc agc gca cag caa ca

#g atc ccc tat aca gtg     2688

Leu Tyr Gly Leu Asp Gly Ser Ala Gln Gln Gl

#n Ile Pro Tyr Thr Val

885

#               890

#               895

act gaa tcc cgt cca cag gtg cgc caa tta ca

#a gat ggc gcc acc gtt     2736

Thr Glu Ser Arg Pro Gln Val Arg Gln Leu Gl

#n Asp Gly Ala Thr Val

900

#           905

#           910

tcc ccg gtg ctc tgg gcc tca gtc gtg gaa ag

#c cgt agt tat cac tac     2784

Ser Pro Val Leu Trp Ala Ser Val Val Glu Se

#r Arg Ser Tyr His Tyr

915

#       920

#       925

gaa cgt att atc agt gat ccc cag tgc aat ca

#g gat atc acg ttg tcc     2832

Glu Arg Ile Ile Ser Asp Pro Gln Cys Asn Gl

#n Asp Ile Thr Leu Ser

930

#   935

#   940

agt gac cta ttc ggg caa cca ctg aaa cag gt

#t tcc gta caa tat ccc     2880

Ser Asp Leu Phe Gly Gln Pro Leu Lys Gln Va

#l Ser Val Gln Tyr Pro

945                 9

#50                 9

#55                 9

#60

cgc cgc aac aaa cca aca acc aat ccg tat cc

#c gat acc cta ccg gat     2928

Arg Arg Asn Lys Pro Thr Thr Asn Pro Tyr Pr

#o Asp Thr Leu Pro Asp

965

#               970

#               975

acg ctg ttt gcc agc agt tat gac gat caa ca

#a cag cta ttg cga tta     2976

Thr Leu Phe Ala Ser Ser Tyr Asp Asp Gln Gl

#n Gln Leu Leu Arg Leu

980

#           985

#           990

acc tgc cga caa tcc agt tgg cac  cat ctt

#att ggt aat  gag cta aga   3024

Thr Cys Arg Gln Ser Ser Trp His  His Leu

#Ile Gly Asn  Glu Leu Arg

995

#       1000

#       1005

gtg ttg  gga tta ccg gat ggc  aca cgc a

#gt gat gcc  ttt act tac      3069

Val Leu  Gly Leu Pro Asp Gly  Thr Arg S

#er Asp Ala  Phe Thr Tyr

1010

#    1015

#    1020

gat gcc  aaa cag gta cct gtc  gat ggc t

#ta aat ctg  gaa acc ctg      3114

Asp Ala  Lys Gln Val Pro Val  Asp Gly L

#eu Asn Leu  Glu Thr Leu

1025

#    1030

#    1035

tgt gct  gaa aat agc ctg att  gcc gat g

#at aaa cct  cgc gaa tac      3159

Cys Ala  Glu Asn Ser Leu Ile  Ala Asp A

#sp Lys Pro  Arg Glu Tyr

1040

#    1045

#    1050

ctc aat  cag caa cga acg ttc  tat acc g

#ac ggg aaa  aac caa aca      3204

Leu Asn  Gln Gln Arg Thr Phe  Tyr Thr A

#sp Gly Lys  Asn Gln Thr

1055

#    1060

#    1065

ccg ctg  aaa aca ccg aca cga  caa gcg t

#ta atc gcc  ttt acc gaa      3249

Pro Leu  Lys Thr Pro Thr Arg  Gln Ala L

#eu Ile Ala  Phe Thr Glu

1070

#    1075

#    1080

acg gcg  gta tta acg gaa tct  ctg tta t

#cc gcg ttt  gat ggc ggt      3294

Thr Ala  Val Leu Thr Glu Ser  Leu Leu S

#er Ala Phe  Asp Gly Gly

1085

#    1090

#    1095

att acg  cca gac gaa tta ccg  gga ata c

#tg aca cag  gcc gga tac      3339

Ile Thr  Pro Asp Glu Leu Pro  Gly Ile L

#eu Thr Gln  Ala Gly Tyr

1100

#    1105

#    1110

caa caa  gag cct tat ctg ttt  cca cgc a

#cc ggc gaa  aac aaa gtt      3384

Gln Gln  Glu Pro Tyr Leu Phe  Pro Arg T

#hr Gly Glu  Asn Lys Val

1115

#    1120

#    1125

tgg gta  gcg cgt caa ggc tat  acc gat t

#ac ggg acg  gaa gca caa      3429

Trp Val  Ala Arg Gln Gly Tyr  Thr Asp T

#yr Gly Thr  Glu Ala Gln

1130

#    1135

#    1140

ttt tgg  cgt cct gtc gca caa  cgt aac a

#gc ctg tta  acc ggg aaa      3474

Phe Trp  Arg Pro Val Ala Gln  Arg Asn S

#er Leu Leu  Thr Gly Lys

1145

#    1150

#    1155

atg acg  tta aaa tgg gat act  cac tat t

#gt gtc atc  acc caa acc      3519

Met Thr  Leu Lys Trp Asp Thr  His Tyr C

#ys Val Ile  Thr Gln Thr

1160

#    1165

#    1170

caa gat  gct gcc ggc ctc acc  gtc tca g

#cc aat tat  gac tgg cgt      3564

Gln Asp  Ala Ala Gly Leu Thr  Val Ser A

#la Asn Tyr  Asp Trp Arg

1175

#    1180

#    1185

ttt ctc  aca cca acg caa ctg  act gac a

#tc aac gat  aat gtg cat      3609

Phe Leu  Thr Pro Thr Gln Leu  Thr Asp I

#le Asn Asp  Asn Val His

1190

#    1195

#    1200

ctc atc  acc ttg gat gct ctg  gga cgc c

#ct gtc acg  caa cgt ttc      3654

Leu Ile  Thr Leu Asp Ala Leu  Gly Arg P

#ro Val Thr  Gln Arg Phe

1205

#    1210

#    1215

tgg ggg  atc gaa agc ggt gtg  gca aca g

#gt tac tct  tca tca gaa      3699

Trp Gly  Ile Glu Ser Gly Val  Ala Thr G

#ly Tyr Ser  Ser Ser Glu

1220

#    1225

#    1230

gaa aaa  cca ttc tct cca cca  aac gat a

#tc gat acc  gct att aat      3744

Glu Lys  Pro Phe Ser Pro Pro  Asn Asp I

#le Asp Thr  Ala Ile Asn

1235

#    1240

#    1245

cta acc  gga cca ctc cct gtc  gca cag t

#gt ctg gtc  tat gca ccg      3789

Leu Thr  Gly Pro Leu Pro Val  Ala Gln C

#ys Leu Val  Tyr Ala Pro

1250

#    1255

#    1260

gac agt  tgg atg cca cta ttc  agt caa g

#aa acc ttc  aac aca tta      3834

Asp Ser  Trp Met Pro Leu Phe  Ser Gln G

#lu Thr Phe  Asn Thr Leu

1265

#    1270

#    1275

acg cag  gaa gag cag gag acg  ctg cgt g

#at tca cgt  att atc acg      3879

Thr Gln  Glu Glu Gln Glu Thr  Leu Arg A

#sp Ser Arg  Ile Ile Thr

1280

#    1285

#    1290

gaa gat  tgg cgt att tgc gca  ctg act c

#gc cgc cgt  tgg cta caa      3924

Glu Asp  Trp Arg Ile Cys Ala  Leu Thr A

#rg Arg Arg  Trp Leu Gln

1295

#    1300

#    1305

agt caa  aag atc agt aca cca  tta gtt a

#aa ctg tta  acc aac agc      3969

Ser Gln  Lys Ile Ser Thr Pro  Leu Val L

#ys Leu Leu  Thr Asn Ser

1310

#    1315

#    1320

att ggt  tta cct ccc cat aac  ctt acg c

#tg acc aca  gac cgt tat      4014

Ile Gly  Leu Pro Pro His Asn  Leu Thr L

#eu Thr Thr  Asp Arg Tyr

1325

#    1330

#    1335

gac cgc  gac tct gag cag caa  att cgc c

#aa caa gtc  gca ttt agt      4059

Asp Arg  Asp Ser Glu Gln Gln  Ile Arg G

#ln Gln Val  Ala Phe Ser

1340

#    1345

#    1350

gat ggt  ttt ggc cgt ctg cta  caa gcg t

#ct gta cga  cat gag gca      4104

Asp Gly  Phe Gly Arg Leu Leu  Gln Ala S

#er Val Arg  His Glu Ala

1355

#    1360

#    1365

ggc gaa  gcc tgg caa cgt aac  caa gac g

#gt tct ctg  gtg aca aaa      4149

Gly Glu  Ala Trp Gln Arg Asn  Gln Asp G

#ly Ser Leu  Val Thr Lys

1370

#    1375

#    1380

gtg gag  aat acc aaa acg cgt  tgg gcg g

#tc acg gga  cgc acc gaa      4194

Val Glu  Asn Thr Lys Thr Arg  Trp Ala V

#al Thr Gly  Arg Thr Glu

1385

#    1390

#    1395

tat gat  aat aaa ggg caa acg  ata cgc a

#ct tat cag  ccc tat ttc      4239

Tyr Asp  Asn Lys Gly Gln Thr  Ile Arg T

#hr Tyr Gln  Pro Tyr Phe

1400

#    1405

#    1410

ctc aac  gac tgg cga tat gtc  agt gat g

#ac agc gcc  aga aaa gaa      4284

Leu Asn  Asp Trp Arg Tyr Val  Ser Asp A

#sp Ser Ala  Arg Lys Glu

1415

#    1420

#    1425

gcc tat  gcg gat act cat att  tat gat c

#ca att ggg  cga gaa atc      4329

Ala Tyr  Ala Asp Thr His Ile  Tyr Asp P

#ro Ile Gly  Arg Glu Ile

1430

#    1435

#    1440

cgg gtt  att act gca aaa ggc  tgg ctg c

#gc caa agc  caa tat ttc      4374

Arg Val  Ile Thr Ala Lys Gly  Trp Leu A

#rg Gln Ser  Gln Tyr Phe

1445

#    1450

#    1455

ccg tgg  ttt acc gtg agt gag  gat gag a

#at gat acg  gcc gct gat      4419

Pro Trp  Phe Thr Val Ser Glu  Asp Glu A

#sn Asp Thr  Ala Ala Asp

1460

#    1465

#    1470

gcg ctg  gtg taa

#

#

#     4431

Ala Leu  Val

1475

<210> SEQ ID NO 4

<211> LENGTH: 1476

<212> TYPE: PRT

<213> ORGANISM: Photorhabdus luminescens

<400> SEQUENCE: 4

Met Gln Asn Ser Gln Thr Phe Ser Val Thr Gl

#u Leu Ser Leu Pro Lys

1               5

#                10

#                15

Gly Gly Gly Ala Ile Thr Gly Met Gly Glu Al

#a Leu Thr Pro Ala Gly

20

#            25

#            30

Pro Asp Gly Met Ala Ala Leu Ser Leu Pro Le

#u Pro Ile Ser Ala Gly

35

#        40

#        45

Arg Gly Tyr Ala Pro Ser Leu Thr Leu Asn Ty

#r Asn Ser Gly Thr Gly

50

#    55

#    60

Asn Ser Pro Phe Gly Leu Gly Trp Asp Cys Gl

#y Val Met Ala Ile Arg

65

#70

#75

#80

Arg Arg Thr Ser Thr Gly Val Pro Asn Tyr As

#p Glu Thr Asp Thr Phe

85

#                90

#                95

Leu Gly Pro Glu Gly Glu Val Leu Val Val Al

#a Leu Asn Glu Ala Gly

100

#           105

#           110

Gln Ala Asp Ile Arg Ser Glu Ser Ser Leu Gl

#n Gly Ile Asn Leu Gly

115

#       120

#       125

Ala Thr Phe Thr Val Thr Cys Tyr Arg Ser Ar

#g Leu Glu Ser His Phe

130

#   135

#   140

Asn Arg Leu Glu Tyr Trp Gln Pro Gln Thr Th

#r Gly Ala Thr Asp Phe

145                 1

#50                 1

#55                 1

#60

Trp Leu Ile Tyr Ser Pro Asp Gly Gln Val Hi

#s Leu Leu Gly Lys Asn

165

#               170

#               175

Pro Gln Ala Arg Ile Ser Asn Pro Leu Asn Va

#l Asn Gln Thr Ala Gln

180

#           185

#           190

Trp Leu Leu Glu Ala Ser Ile Ser Ser His Se

#r Glu Gln Ile Tyr Tyr

195

#       200

#       205

Gln Tyr Arg Ala Glu Asp Glu Ala Gly Cys Gl

#u Thr Asp Glu Leu Ala

210

#   215

#   220

Ala His Pro Ser Ala Thr Val Gln Arg Tyr Le

#u Gln Thr Val His Tyr

225                 2

#30                 2

#35                 2

#40

Gly Asn Leu Thr Ala Ser Asp Val Phe Pro Th

#r Leu Asn Gly Asp Asp

245

#               250

#               255

Pro Leu Lys Ser Gly Trp Met Phe Cys Leu Va

#l Phe Asp Tyr Gly Glu

260

#           265

#           270

Arg Lys Asn Ser Leu Ser Glu Met Pro Leu Ph

#e Lys Ala Thr Gly Asn

275

#       280

#       285

Trp Leu Cys Arg Lys Asp Arg Phe Ser Arg Ty

#r Glu Tyr Gly Phe Glu

290

#   295

#   300

Leu Arg Thr Arg Arg Leu Cys Arg Gln Ile Le

#u Met Phe His Arg Leu

305                 3

#10                 3

#15                 3

#20

Gln Thr Leu Ser Gly Gln Ala Lys Gly Asp As

#p Glu Pro Ala Leu Val

325

#               330

#               335

Ser Arg Leu Ile Leu Asp Tyr Asp Glu Asn Al

#a Met Val Ser Thr Leu

340

#           345

#           350

Val Ser Val Arg Arg Val Gly His Glu Asp As

#n Asn Thr Val Thr Ala

355

#       360

#       365

Leu Pro Pro Leu Glu Leu Ala Tyr Gln Pro Ph

#e Glu Pro Glu Gln Thr

370

#   375

#   380

Ala Leu Trp Gln Ser Met Asp Val Leu Ala As

#n Phe Asn Thr Ile Gln

385                 3

#90                 3

#95                 4

#00

Arg Trp Gln Leu Leu Asp Leu Lys Gly Glu Gl

#y Val Pro Gly Ile Leu

405

#               410

#               415

Tyr Gln Asp Arg Asn Gly Trp Trp Tyr Arg Se

#r Ala Gln Arg Gln Ala

420

#           425

#           430

Gly Glu Glu Met Asn Ala Val Thr Trp Gly Ly

#s Met Gln Leu Leu Pro

435

#       440

#       445

Ile Thr Pro Ala Val Gln Asp Asn Ala Ser Le

#u Met Asp Ile Asn Gly

450

#   455

#   460

Asp Gly Gln Leu Asp Trp Val Ile Thr Gly Pr

#o Gly Leu Arg Gly Tyr

465                 4

#70                 4

#75                 4

#80

His Ser Gln His Pro Asp Gly Ser Trp Thr Ar

#g Phe Thr Pro Leu His

485

#               490

#               495

Ala Leu Pro Ile Glu Tyr Ser His Pro Arg Al

#a Gln Leu Ala Asp Leu

500

#           505

#           510

Met Gly Ala Gly Leu Ser Asp Leu Val Leu Il

#e Gly Pro Lys Ser Val

515

#       520

#       525

Arg Leu Tyr Val Asn Asn Arg Asp Gly Phe Th

#r Glu Gly Arg Asp Val

530

#   535

#   540

Val Gln Ser Gly Asp Ile Thr Leu Pro Leu Pr

#o Gly Ala Asp Ala Arg

545                 5

#50                 5

#55                 5

#60

Lys Leu Val Ala Phe Ser Asp Val Leu Gly Se

#r Gly Gln Ala His Leu

565

#               570

#               575

Val Glu Val Ser Ala Thr Gln Val Thr Cys Tr

#p Pro Asn Leu Gly His

580

#           585

#           590

Gly Arg Phe Gly Gln Pro Ile Val Leu Pro Gl

#y Phe Ser Gln Ser Ala

595

#       600

#       605

Ala Ser Phe Asn Pro Asp Arg Val His Leu Al

#a Asp Leu Asp Gly Ser

610

#   615

#   620

Gly Pro Ala Asp Leu Ile Tyr Val His Ala As

#p Arg Leu Asp Ile Phe

625                 6

#30                 6

#35                 6

#40

Ser Asn Glu Ser Gly Asn Gly Phe Ala Lys Pr

#o Phe Thr Leu Ser Phe

645

#               650

#               655

Pro Asp Gly Leu Arg Phe Asp Asp Thr Cys Gl

#n Leu Gln Val Ala Asp

660

#           665

#           670

Val Gln Gly Leu Gly Val Val Ser Leu Ile Le

#u Ser Val Pro His Met

675

#       680

#       685

Ala Pro His His Trp Arg Cys Asp Leu Thr As

#n Ala Lys Pro Trp Leu

690

#   695

#   700

Leu Ser Glu Thr Asn Asn Asn Met Gly Ala As

#n His Thr Leu His Tyr

705                 7

#10                 7

#15                 7

#20

Arg Ser Ser Val Gln Phe Trp Leu Asp Glu Ly

#s Ala Ala Ala Leu Ala

725

#               730

#               735

Thr Gly Gln Thr Pro Val Cys Tyr Leu Pro Ph

#e Pro Val His Thr Leu

740

#           745

#           750

Trp Gln Thr Glu Thr Glu Asp Glu Ile Ser Gl

#y Asn Lys Leu Val Thr

755

#       760

#       765

Thr Leu Arg Tyr Ala His Gly Ala Trp Asp Gl

#y Arg Glu Arg Glu Phe

770

#   775

#   780

Arg Gly Phe Gly Tyr Val Glu Gln Thr Asp Se

#r His Gln Leu Ala Gln

785                 7

#90                 7

#95                 8

#00

Gly Asn Ala Pro Glu Arg Thr Pro Pro Ala Le

#u Thr Lys Ser Trp Tyr

805

#               810

#               815

Ala Thr Gly Leu Pro Ala Val Asp Asn Ala Le

#u Ser Ala Gly Tyr Trp

820

#           825

#           830

Arg Gly Asp Lys Gln Ala Phe Ala Gly Phe Th

#r Pro Arg Phe Thr Leu

835

#       840

#       845

Trp Lys Glu Gly Lys Asp Val Pro Leu Thr Pr

#o Glu Asp Asp His Asn

850

#   855

#   860

Leu Tyr Trp Leu Asn Arg Ala Leu Lys Gly Gl

#n Pro Leu Arg Ser Glu

865                 8

#70                 8

#75                 8

#80

Leu Tyr Gly Leu Asp Gly Ser Ala Gln Gln Gl

#n Ile Pro Tyr Thr Val

885

#               890

#               895

Thr Glu Ser Arg Pro Gln Val Arg Gln Leu Gl

#n Asp Gly Ala Thr Val

900

#           905

#           910

Ser Pro Val Leu Trp Ala Ser Val Val Glu Se

#r Arg Ser Tyr His Tyr

915

#       920

#       925

Glu Arg Ile Ile Ser Asp Pro Gln Cys Asn Gl

#n Asp Ile Thr Leu Ser

930

#   935

#   940

Ser Asp Leu Phe Gly Gln Pro Leu Lys Gln Va

#l Ser Val Gln Tyr Pro

945                 9

#50                 9

#55                 9

#60

Arg Arg Asn Lys Pro Thr Thr Asn Pro Tyr Pr

#o Asp Thr Leu Pro Asp

965

#               970

#               975

Thr Leu Phe Ala Ser Ser Tyr Asp Asp Gln Gl

#n Gln Leu Leu Arg Leu

980

#           985

#           990

Thr Cys Arg Gln Ser Ser Trp His  His Leu

#Ile Gly Asn  Glu Leu Arg

995

#       1000

#       1005

Val Leu  Gly Leu Pro Asp Gly  Thr Arg S

#er Asp Ala  Phe Thr Tyr

1010

#    1015

#    1020

Asp Ala  Lys Gln Val Pro Val  Asp Gly L

#eu Asn Leu  Glu Thr Leu

1025

#    1030

#    1035

Cys Ala  Glu Asn Ser Leu Ile  Ala Asp A

#sp Lys Pro  Arg Glu Tyr

1040

#    1045

#    1050

Leu Asn  Gln Gln Arg Thr Phe  Tyr Thr A

#sp Gly Lys  Asn Gln Thr

1055

#    1060

#    1065

Pro Leu  Lys Thr Pro Thr Arg  Gln Ala L

#eu Ile Ala  Phe Thr Glu

1070

#    1075

#    1080

Thr Ala  Val Leu Thr Glu Ser  Leu Leu S

#er Ala Phe  Asp Gly Gly

1085

#    1090

#    1095

Ile Thr  Pro Asp Glu Leu Pro  Gly Ile L

#eu Thr Gln  Ala Gly Tyr

1100

#    1105

#    1110

Gln Gln  Glu Pro Tyr Leu Phe  Pro Arg T

#hr Gly Glu  Asn Lys Val

1115

#    1120

#    1125

Trp Val  Ala Arg Gln Gly Tyr  Thr Asp T

#yr Gly Thr  Glu Ala Gln

1130

#    1135

#    1140

Phe Trp  Arg Pro Val Ala Gln  Arg Asn S

#er Leu Leu  Thr Gly Lys

1145

#    1150

#    1155

Met Thr  Leu Lys Trp Asp Thr  His Tyr C

#ys Val Ile  Thr Gln Thr

1160

#    1165

#    1170

Gln Asp  Ala Ala Gly Leu Thr  Val Ser A

#la Asn Tyr  Asp Trp Arg

1175

#    1180

#    1185

Phe Leu  Thr Pro Thr Gln Leu  Thr Asp I

#le Asn Asp  Asn Val His

1190

#    1195

#    1200

Leu Ile  Thr Leu Asp Ala Leu  Gly Arg P

#ro Val Thr  Gln Arg Phe

1205

#    1210

#    1215

Trp Gly  Ile Glu Ser Gly Val  Ala Thr G

#ly Tyr Ser  Ser Ser Glu

1220

#    1225

#    1230

Glu Lys  Pro Phe Ser Pro Pro  Asn Asp I

#le Asp Thr  Ala Ile Asn

1235

#    1240

#    1245

Leu Thr  Gly Pro Leu Pro Val  Ala Gln C

#ys Leu Val  Tyr Ala Pro

1250

#    1255

#    1260

Asp Ser  Trp Met Pro Leu Phe  Ser Gln G

#lu Thr Phe  Asn Thr Leu

1265

#    1270

#    1275

Thr Gln  Glu Glu Gln Glu Thr  Leu Arg A

#sp Ser Arg  Ile Ile Thr

1280

#    1285

#    1290

Glu Asp  Trp Arg Ile Cys Ala  Leu Thr A

#rg Arg Arg  Trp Leu Gln

1295

#    1300

#    1305

Ser Gln  Lys Ile Ser Thr Pro  Leu Val L

#ys Leu Leu  Thr Asn Ser

1310

#    1315

#    1320

Ile Gly  Leu Pro Pro His Asn  Leu Thr L

#eu Thr Thr  Asp Arg Tyr

1325

#    1330

#    1335

Asp Arg  Asp Ser Glu Gln Gln  Ile Arg G

#ln Gln Val  Ala Phe Ser

1340

#    1345

#    1350

Asp Gly  Phe Gly Arg Leu Leu  Gln Ala S

#er Val Arg  His Glu Ala

1355

#    1360

#    1365

Gly Glu  Ala Trp Gln Arg Asn  Gln Asp G

#ly Ser Leu  Val Thr Lys

1370

#    1375

#    1380

Val Glu  Asn Thr Lys Thr Arg  Trp Ala V

#al Thr Gly  Arg Thr Glu

1385

#    1390

#    1395

Tyr Asp  Asn Lys Gly Gln Thr  Ile Arg T

#hr Tyr Gln  Pro Tyr Phe

1400

#    1405

#    1410

Leu Asn  Asp Trp Arg Tyr Val  Ser Asp A

#sp Ser Ala  Arg Lys Glu

1415

#    1420

#    1425

Ala Tyr  Ala Asp Thr His Ile  Tyr Asp P

#ro Ile Gly  Arg Glu Ile

1430

#    1435

#    1440

Arg Val  Ile Thr Ala Lys Gly  Trp Leu A

#rg Gln Ser  Gln Tyr Phe

1445

#    1450

#    1455

Pro Trp  Phe Thr Val Ser Glu  Asp Glu A

#sn Asp Thr  Ala Ala Asp

1460

#    1465

#    1470

Ala Leu  Val

1475

<210> SEQ ID NO 5

<211> LENGTH: 2745

<212> TYPE: DNA

<213> ORGANISM: Photorhabdus luminescens

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(2745)

<400> SEQUENCE: 5

atg agc agt tac aat tct gca att gac caa aa

#g acc ccc tcg att aag       48

Met Ser Ser Tyr Asn Ser Ala Ile Asp Gln Ly

#s Thr Pro Ser Ile Lys

1               5

#                10

#                15

gta tta gat aac agg aaa tta aat gta cgt ac

#t tta gaa tat cta cgc       96

Val Leu Asp Asn Arg Lys Leu Asn Val Arg Th

#r Leu Glu Tyr Leu Arg

20

#            25

#            30

act caa gct gac gaa aac agt gat gaa tta at

#t acg ttc tat gag ttc      144

Thr Gln Ala Asp Glu Asn Ser Asp Glu Leu Il

#e Thr Phe Tyr Glu Phe

35

#        40

#        45

aat att ccg gga ttt cag gta aaa agc acc ga

#t cct cgt aaa aat aaa      192

Asn Ile Pro Gly Phe Gln Val Lys Ser Thr As

#p Pro Arg Lys Asn Lys

50

#    55

#    60

aac cag agc ggc cca aat ttc att cgt gtc tt

#t aat ctt gcc ggt caa      240

Asn Gln Ser Gly Pro Asn Phe Ile Arg Val Ph

#e Asn Leu Ala Gly Gln

65

#70

#75

#80

gtt tta cgt gaa gaa agt gtt gat gcc ggt cg

#g act att acc ctc aat      288

Val Leu Arg Glu Glu Ser Val Asp Ala Gly Ar

#g Thr Ile Thr Leu Asn

85

#                90

#                95

gat att gaa agt cgc ccg gtg ttg atc atc aa

#t gca acc ggt gtc cgc      336

Asp Ile Glu Ser Arg Pro Val Leu Ile Ile As

#n Ala Thr Gly Val Arg

100

#           105

#           110

caa aac cat cgt tat gaa gat aac acc ctt cc

#c ggt cgt ctg ctc gct      384

Gln Asn His Arg Tyr Glu Asp Asn Thr Leu Pr

#o Gly Arg Leu Leu Ala

115

#       120

#       125

atc acc gaa caa gta cag gca gga gag aaa ac

#g acc gaa cgt ctt atc      432

Ile Thr Glu Gln Val Gln Ala Gly Glu Lys Th

#r Thr Glu Arg Leu Ile

130

#   135

#   140

tgg gcc ggc aat acg ccg caa gaa aaa gat ta

#c aac ctc gcc ggt cag      480

Trp Ala Gly Asn Thr Pro Gln Glu Lys Asp Ty

#r Asn Leu Ala Gly Gln

145                 1

#50                 1

#55                 1

#60

tgt gtc cgc cat tac gat acc gcg gga ctt ac

#t caa ctc aat agc ctt      528

Cys Val Arg His Tyr Asp Thr Ala Gly Leu Th

#r Gln Leu Asn Ser Leu

165

#               170

#               175

tct ctg gct ggc gtc gtg cta tca caa tct ca

#a caa ctg ctt acc gat      576

Ser Leu Ala Gly Val Val Leu Ser Gln Ser Gl

#n Gln Leu Leu Thr Asp

180

#           185

#           190

aac cag gat gcc gac tgg aca ggt gaa gac ca

#g agc ctc tgg caa caa      624

Asn Gln Asp Ala Asp Trp Thr Gly Glu Asp Gl

#n Ser Leu Trp Gln Gln

195

#       200

#       205

aaa ctg agt agt gat gtc tat atc acc caa ag

#t aac act gat gcc acc      672

Lys Leu Ser Ser Asp Val Tyr Ile Thr Gln Se

#r Asn Thr Asp Ala Thr

210

#   215

#   220

ggg gct tta ctg acc cag acc gat gcc aaa gg

#c aac att cag cgg ctg      720

Gly Ala Leu Leu Thr Gln Thr Asp Ala Lys Gl

#y Asn Ile Gln Arg Leu

225                 2

#30                 2

#35                 2

#40

gcc tat gat gtg gcc ggg cag cta aaa ggg ag

#t tgg tta aca ctc aaa      768

Ala Tyr Asp Val Ala Gly Gln Leu Lys Gly Se

#r Trp Leu Thr Leu Lys

245

#               250

#               255

ggt cag gcg gaa cag gtg att atc aaa tcg ct

#a acc tac tcc gcc gcc      816

Gly Gln Ala Glu Gln Val Ile Ile Lys Ser Le

#u Thr Tyr Ser Ala Ala

260

#           265

#           270

ggg caa aaa tta cgt gaa gag cac ggt aac gg

#g att gtc act gaa tac      864

Gly Gln Lys Leu Arg Glu Glu His Gly Asn Gl

#y Ile Val Thr Glu Tyr

275

#       280

#       285

agc tac gaa ccg gaa acc caa cgg ctt atc gg

#c att acc act cgc cgt      912

Ser Tyr Glu Pro Glu Thr Gln Arg Leu Ile Gl

#y Ile Thr Thr Arg Arg

290

#   295

#   300

cca tca gac gcc aag gtg ttg caa gac cta cg

#c tat caa tat gac cca      960

Pro Ser Asp Ala Lys Val Leu Gln Asp Leu Ar

#g Tyr Gln Tyr Asp Pro

305                 3

#10                 3

#15                 3

#20

gta ggc aat gtc att aat atc cgt aat gat gc

#g gaa gcc act cgc ttt     1008

Val Gly Asn Val Ile Asn Ile Arg Asn Asp Al

#a Glu Ala Thr Arg Phe

325

#               330

#               335

tgg cgc aat cag aaa gta gcc ccg gag aat ag

#c tat acc tac gat tcc     1056

Trp Arg Asn Gln Lys Val Ala Pro Glu Asn Se

#r Tyr Thr Tyr Asp Ser

340

#           345

#           350

ctg tat cag ctt atc agc gcc acc ggg cgc ga

#a atg gcc aat atc ggt     1104

Leu Tyr Gln Leu Ile Ser Ala Thr Gly Arg Gl

#u Met Ala Asn Ile Gly

355

#       360

#       365

cag caa aac aac caa ctt ccc tcc cct gcg ct

#a cct tct gac aac aat     1152

Gln Gln Asn Asn Gln Leu Pro Ser Pro Ala Le

#u Pro Ser Asp Asn Asn

370

#   375

#   380

acc tac act aac tat act cgc agc tac agc ta

#t gat cac agt ggt aat     1200

Thr Tyr Thr Asn Tyr Thr Arg Ser Tyr Ser Ty

#r Asp His Ser Gly Asn

385                 3

#90                 3

#95                 4

#00

ctg acg caa att cgg cac agc tcg cca gct ac

#c cag aac aac tac acc     1248

Leu Thr Gln Ile Arg His Ser Ser Pro Ala Th

#r Gln Asn Asn Tyr Thr

405

#               410

#               415

gtg gct atc acc ctc tca aac cgc agc aat cg

#g ggt gtt ctc agt acg     1296

Val Ala Ile Thr Leu Ser Asn Arg Ser Asn Ar

#g Gly Val Leu Ser Thr

420

#           425

#           430

cta acc acc gat cca aat caa gtg gat acg tt

#g ttt gat gcc ggt ggt     1344

Leu Thr Thr Asp Pro Asn Gln Val Asp Thr Le

#u Phe Asp Ala Gly Gly

435

#       440

#       445

cac caa acc agt tta tta ccc gga cag aca ct

#t atc tgg aca cca cga     1392

His Gln Thr Ser Leu Leu Pro Gly Gln Thr Le

#u Ile Trp Thr Pro Arg

450

#   455

#   460

gga gag tta aag cag gtt aat aat ggc ccg gg

#a aat gag tgg tac cgc     1440

Gly Glu Leu Lys Gln Val Asn Asn Gly Pro Gl

#y Asn Glu Trp Tyr Arg

465                 4

#70                 4

#75                 4

#80

tac gac agc aac ggc atg aga caa ctg aaa gt

#g agt gaa cag cca acc     1488

Tyr Asp Ser Asn Gly Met Arg Gln Leu Lys Va

#l Ser Glu Gln Pro Thr

485

#               490

#               495

cag aat act acg cag caa caa cgg gta atc ta

#t ttg ccg gga ctg gag     1536

Gln Asn Thr Thr Gln Gln Gln Arg Val Ile Ty

#r Leu Pro Gly Leu Glu

500

#           505

#           510

cta cgc aca acc cag agc aac gcc aca aca ac

#g gaa gag tta cac gtt     1584

Leu Arg Thr Thr Gln Ser Asn Ala Thr Thr Th

#r Glu Glu Leu His Val

515

#       520

#       525

atc aca ctc ggt gaa gcc ggt cgc gca cag gt

#a cgg gtg ttg cac tgg     1632

Ile Thr Leu Gly Glu Ala Gly Arg Ala Gln Va

#l Arg Val Leu His Trp

530

#   535

#   540

gag agc ggt aag cca gaa gat gtc aac aat aa

#t caa cta cgt tac agc     1680

Glu Ser Gly Lys Pro Glu Asp Val Asn Asn As

#n Gln Leu Arg Tyr Ser

545                 5

#50                 5

#55                 5

#60

tac gat aat ctg atc ggc tcc agc cag ctt ga

#a ctg gac aac caa gga     1728

Tyr Asp Asn Leu Ile Gly Ser Ser Gln Leu Gl

#u Leu Asp Asn Gln Gly

565

#               570

#               575

caa att atc agc gag gaa gag tat tat cca tt

#t ggc ggg aca gcg ctg     1776

Gln Ile Ile Ser Glu Glu Glu Tyr Tyr Pro Ph

#e Gly Gly Thr Ala Leu

580

#           585

#           590

tgg gca gca aac agc caa aca gaa gcc agc ta

#t aaa acg att cgc tat     1824

Trp Ala Ala Asn Ser Gln Thr Glu Ala Ser Ty

#r Lys Thr Ile Arg Tyr

595

#       600

#       605

tcc ggc aaa gaa cga gat gcc acc ggg ttg ta

#t tat tac ggt tat cgt     1872

Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Ty

#r Tyr Tyr Gly Tyr Arg

610

#   615

#   620

tat tac caa ccg tgg gcg ggc aga tgg tta ag

#c gcg gac ccg gca gga     1920

Tyr Tyr Gln Pro Trp Ala Gly Arg Trp Leu Se

#r Ala Asp Pro Ala Gly

625                 6

#30                 6

#35                 6

#40

acc att gat ggg ctg aat cta tac cga atg gt

#a aga aat aat cct gtg     1968

Thr Ile Asp Gly Leu Asn Leu Tyr Arg Met Va

#l Arg Asn Asn Pro Val

645

#               650

#               655

agt tta caa gat gaa aat gga tta gcg cca ga

#a aaa ggg aaa tat acc     2016

Ser Leu Gln Asp Glu Asn Gly Leu Ala Pro Gl

#u Lys Gly Lys Tyr Thr

660

#           665

#           670

aaa gag gta aat ttc ttt gat gaa tta aaa tt

#c aaa ttg gca gcc aaa     2064

Lys Glu Val Asn Phe Phe Asp Glu Leu Lys Ph

#e Lys Leu Ala Ala Lys

675

#       680

#       685

agt tca cat gtt gtc aaa tgg aac gag aaa ga

#g agc agt tat aca aaa     2112

Ser Ser His Val Val Lys Trp Asn Glu Lys Gl

#u Ser Ser Tyr Thr Lys

690

#   695

#   700

aat aaa tca ttg aaa gtg gtt cgt gtc ggt ga

#t tcc gat ccg tcg ggt     2160

Asn Lys Ser Leu Lys Val Val Arg Val Gly As

#p Ser Asp Pro Ser Gly

705                 7

#10                 7

#15                 7

#20

tat ttg cta agc cac gaa gag tta cta aaa gg

#t ata gaa aaa agt caa     2208

Tyr Leu Leu Ser His Glu Glu Leu Leu Lys Gl

#y Ile Glu Lys Ser Gln

725

#               730

#               735

atc ata tat agc cga ctt gaa gaa aac agc tc

#c ctt tca gaa aaa tca     2256

Ile Ile Tyr Ser Arg Leu Glu Glu Asn Ser Se

#r Leu Ser Glu Lys Ser

740

#           745

#           750

aaa acg aat ctt tct tta gga tct gaa ata tc

#c ggt tat atg gca aga     2304

Lys Thr Asn Leu Ser Leu Gly Ser Glu Ile Se

#r Gly Tyr Met Ala Arg

755

#       760

#       765

acc ata caa gat acg ata tca gaa tat gcc ga

#a gag cat aaa tat aga     2352

Thr Ile Gln Asp Thr Ile Ser Glu Tyr Ala Gl

#u Glu His Lys Tyr Arg

770

#   775

#   780

agt aat cac cct gat ttt tat tca gaa acc ga

#t ttc ttt gcg tta atg     2400

Ser Asn His Pro Asp Phe Tyr Ser Glu Thr As

#p Phe Phe Ala Leu Met

785                 7

#90                 7

#95                 8

#00

gat aaa agt gaa aaa aat gat tat tcc ggt ga

#a aga aaa att tat gcg     2448

Asp Lys Ser Glu Lys Asn Asp Tyr Ser Gly Gl

#u Arg Lys Ile Tyr Ala

805

#               810

#               815

gca atg gag gtt aag gtt tat cat gat tta aa

#a aat aaa caa tca gaa     2496

Ala Met Glu Val Lys Val Tyr His Asp Leu Ly

#s Asn Lys Gln Ser Glu

820

#           825

#           830

tta cat gtc aac tat gca ttg gcc cat ccc ta

#t acg caa ttg agt aat     2544

Leu His Val Asn Tyr Ala Leu Ala His Pro Ty

#r Thr Gln Leu Ser Asn

835

#       840

#       845

gaa gaa aga gcg ctg ttg caa gaa aca gaa cc

#c gct att gca ata gat     2592

Glu Glu Arg Ala Leu Leu Gln Glu Thr Glu Pr

#o Ala Ile Ala Ile Asp

850

#   855

#   860

aga gaa tat aat ttc aaa ggt gtt ggc aaa tt

#c ctg aca atg aaa gca     2640

Arg Glu Tyr Asn Phe Lys Gly Val Gly Lys Ph

#e Leu Thr Met Lys Ala

865                 8

#70                 8

#75                 8

#80

att aaa aaa tca ttg aaa gga cat aaa att aa

#t agg ata tca aca gag     2688

Ile Lys Lys Ser Leu Lys Gly His Lys Ile As

#n Arg Ile Ser Thr Glu

885

#               890

#               895

gct att aat att cgc tct gcg gct atc gct ga

#g aat tta gga atg cgg     2736

Ala Ile Asn Ile Arg Ser Ala Ala Ile Ala Gl

#u Asn Leu Gly Met Arg

900

#           905

#           910

aga act tca

#

#

#       2745

Arg Thr Ser

915

<210> SEQ ID NO 6

<211> LENGTH: 915

<212> TYPE: PRT

<213> ORGANISM: Photorhabdus luminescens

<400> SEQUENCE: 6

Met Ser Ser Tyr Asn Ser Ala Ile Asp Gln Ly

#s Thr Pro Ser Ile Lys

1               5

#                10

#                15

Val Leu Asp Asn Arg Lys Leu Asn Val Arg Th

#r Leu Glu Tyr Leu Arg

20

#            25

#            30

Thr Gln Ala Asp Glu Asn Ser Asp Glu Leu Il

#e Thr Phe Tyr Glu Phe

35

#        40

#        45

Asn Ile Pro Gly Phe Gln Val Lys Ser Thr As

#p Pro Arg Lys Asn Lys

50

#    55

#    60

Asn Gln Ser Gly Pro Asn Phe Ile Arg Val Ph

#e Asn Leu Ala Gly Gln

65

#70

#75

#80

Val Leu Arg Glu Glu Ser Val Asp Ala Gly Ar

#g Thr Ile Thr Leu Asn

85

#                90

#                95

Asp Ile Glu Ser Arg Pro Val Leu Ile Ile As

#n Ala Thr Gly Val Arg

100

#           105

#           110

Gln Asn His Arg Tyr Glu Asp Asn Thr Leu Pr

#o Gly Arg Leu Leu Ala

115

#       120

#       125

Ile Thr Glu Gln Val Gln Ala Gly Glu Lys Th

#r Thr Glu Arg Leu Ile

130

#   135

#   140

Trp Ala Gly Asn Thr Pro Gln Glu Lys Asp Ty

#r Asn Leu Ala Gly Gln

145                 1

#50                 1

#55                 1

#60

Cys Val Arg His Tyr Asp Thr Ala Gly Leu Th

#r Gln Leu Asn Ser Leu

165

#               170

#               175

Ser Leu Ala Gly Val Val Leu Ser Gln Ser Gl

#n Gln Leu Leu Thr Asp

180

#           185

#           190

Asn Gln Asp Ala Asp Trp Thr Gly Glu Asp Gl

#n Ser Leu Trp Gln Gln

195

#       200

#       205

Lys Leu Ser Ser Asp Val Tyr Ile Thr Gln Se

#r Asn Thr Asp Ala Thr

210

#   215

#   220

Gly Ala Leu Leu Thr Gln Thr Asp Ala Lys Gl

#y Asn Ile Gln Arg Leu

225                 2

#30                 2

#35                 2

#40

Ala Tyr Asp Val Ala Gly Gln Leu Lys Gly Se

#r Trp Leu Thr Leu Lys

245

#               250

#               255

Gly Gln Ala Glu Gln Val Ile Ile Lys Ser Le

#u Thr Tyr Ser Ala Ala

260

#           265

#           270

Gly Gln Lys Leu Arg Glu Glu His Gly Asn Gl

#y Ile Val Thr Glu Tyr

275

#       280

#       285

Ser Tyr Glu Pro Glu Thr Gln Arg Leu Ile Gl

#y Ile Thr Thr Arg Arg

290

#   295

#   300

Pro Ser Asp Ala Lys Val Leu Gln Asp Leu Ar

#g Tyr Gln Tyr Asp Pro

305                 3

#10                 3

#15                 3

#20

Val Gly Asn Val Ile Asn Ile Arg Asn Asp Al

#a Glu Ala Thr Arg Phe

325

#               330

#               335

Trp Arg Asn Gln Lys Val Ala Pro Glu Asn Se

#r Tyr Thr Tyr Asp Ser

340

#           345

#           350

Leu Tyr Gln Leu Ile Ser Ala Thr Gly Arg Gl

#u Met Ala Asn Ile Gly

355

#       360

#       365

Gln Gln Asn Asn Gln Leu Pro Ser Pro Ala Le

#u Pro Ser Asp Asn Asn

370

#   375

#   380

Thr Tyr Thr Asn Tyr Thr Arg Ser Tyr Ser Ty

#r Asp His Ser Gly Asn

385                 3

#90                 3

#95                 4

#00

Leu Thr Gln Ile Arg His Ser Ser Pro Ala Th

#r Gln Asn Asn Tyr Thr

405

#               410

#               415

Val Ala Ile Thr Leu Ser Asn Arg Ser Asn Ar

#g Gly Val Leu Ser Thr

420

#           425

#           430

Leu Thr Thr Asp Pro Asn Gln Val Asp Thr Le

#u Phe Asp Ala Gly Gly

435

#       440

#       445

His Gln Thr Ser Leu Leu Pro Gly Gln Thr Le

#u Ile Trp Thr Pro Arg

450

#   455

#   460

Gly Glu Leu Lys Gln Val Asn Asn Gly Pro Gl

#y Asn Glu Trp Tyr Arg

465                 4

#70                 4

#75                 4

#80

Tyr Asp Ser Asn Gly Met Arg Gln Leu Lys Va

#l Ser Glu Gln Pro Thr

485

#               490

#               495

Gln Asn Thr Thr Gln Gln Gln Arg Val Ile Ty

#r Leu Pro Gly Leu Glu

500

#           505

#           510

Leu Arg Thr Thr Gln Ser Asn Ala Thr Thr Th

#r Glu Glu Leu His Val

515

#       520

#       525

Ile Thr Leu Gly Glu Ala Gly Arg Ala Gln Va

#l Arg Val Leu His Trp

530

#   535

#   540

Glu Ser Gly Lys Pro Glu Asp Val Asn Asn As

#n Gln Leu Arg Tyr Ser

545                 5

#50                 5

#55                 5

#60

Tyr Asp Asn Leu Ile Gly Ser Ser Gln Leu Gl

#u Leu Asp Asn Gln Gly

565

#               570

#               575

Gln Ile Ile Ser Glu Glu Glu Tyr Tyr Pro Ph

#e Gly Gly Thr Ala Leu

580

#           585

#           590

Trp Ala Ala Asn Ser Gln Thr Glu Ala Ser Ty

#r Lys Thr Ile Arg Tyr

595

#       600

#       605

Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Ty

#r Tyr Tyr Gly Tyr Arg

610

#   615

#   620

Tyr Tyr Gln Pro Trp Ala Gly Arg Trp Leu Se

#r Ala Asp Pro Ala Gly

625                 6

#30                 6

#35                 6

#40

Thr Ile Asp Gly Leu Asn Leu Tyr Arg Met Va

#l Arg Asn Asn Pro Val

645

#               650

#               655

Ser Leu Gln Asp Glu Asn Gly Leu Ala Pro Gl

#u Lys Gly Lys Tyr Thr

660

#           665

#           670

Lys Glu Val Asn Phe Phe Asp Glu Leu Lys Ph

#e Lys Leu Ala Ala Lys

675

#       680

#       685

Ser Ser His Val Val Lys Trp Asn Glu Lys Gl

#u Ser Ser Tyr Thr Lys

690

#   695

#   700

Asn Lys Ser Leu Lys Val Val Arg Val Gly As

#p Ser Asp Pro Ser Gly

705                 7

#10                 7

#15                 7

#20

Tyr Leu Leu Ser His Glu Glu Leu Leu Lys Gl

#y Ile Glu Lys Ser Gln

725

#               730

#               735

Ile Ile Tyr Ser Arg Leu Glu Glu Asn Ser Se

#r Leu Ser Glu Lys Ser

740

#           745

#           750

Lys Thr Asn Leu Ser Leu Gly Ser Glu Ile Se

#r Gly Tyr Met Ala Arg

755

#       760

#       765

Thr Ile Gln Asp Thr Ile Ser Glu Tyr Ala Gl

#u Glu His Lys Tyr Arg

770

#   775

#   780

Ser Asn His Pro Asp Phe Tyr Ser Glu Thr As

#p Phe Phe Ala Leu Met

785                 7

#90                 7

#95                 8

#00

Asp Lys Ser Glu Lys Asn Asp Tyr Ser Gly Gl

#u Arg Lys Ile Tyr Ala

805

#               810

#               815

Ala Met Glu Val Lys Val Tyr His Asp Leu Ly

#s Asn Lys Gln Ser Glu

820

#           825

#           830

Leu His Val Asn Tyr Ala Leu Ala His Pro Ty

#r Thr Gln Leu Ser Asn

835

#       840

#       845

Glu Glu Arg Ala Leu Leu Gln Glu Thr Glu Pr

#o Ala Ile Ala Ile Asp

850

#   855

#   860

Arg Glu Tyr Asn Phe Lys Gly Val Gly Lys Ph

#e Leu Thr Met Lys Ala

865                 8

#70                 8

#75                 8

#80

Ile Lys Lys Ser Leu Lys Gly His Lys Ile As

#n Arg Ile Ser Thr Glu

885

#               890

#               895

Ala Ile Asn Ile Arg Ser Ala Ala Ile Ala Gl

#u Asn Leu Gly Met Arg

900

#           905

#           910

Arg Thr Ser

915

<210> SEQ ID NO 7

<211> LENGTH: 7512

<212> TYPE: DNA

<213> ORGANISM: Photorhabdus luminescens

<400> SEQUENCE: 7

atgcaaaact cattatcaag cactatcgat actatttgtc agaaactgca at

#taacttgt     60

ccggcggaaa ttgctttgta tccctttgat actttccggg aaaaaactcg gg

#gaatggtt    120

aattgggggg aagcaaaacg gatttatgaa attgcacaag cggaacagga ta

#gaaaccta    180

cttcatgaaa aacgtatttt tgcctatgct aatccgctgc tgaaaaacgc tg

#ttcggttg    240

ggtacccggc aaatgttggg ttttatacaa ggttatagtg atctgtttgg ta

#atcgtgct    300

gataactatg ccgcgccggg ctcggttgca tcgatgttct caccggcggc tt

#atttgacg    360

gaattgtacc gtgaagccaa aaacttgcat gacagcagct caatttatta cc

#tagataaa    420

cgtcgcccgg atttagcaag cttaatgctc agccagaaaa atatggatga gg

#aaatttca    480

acgctggctc tctctaatga attgtgcctt gccgggatcg aaacaaaaac ag

#gaaaatca    540

caagatgaag tgatggatat gttgtcaact tatcgtttaa gtggagagac ac

#cttatcat    600

cacgcttatg aaactgttcg tgaaatcgtt catgaacgtg atccaggatt tc

#gtcatttg    660

tcacaggcac ccattgttgc tgctaagctc gatcctgtga ctttgttggg ta

#ttagctcc    720

catatttcgc cagaactgta taacttgctg attgaggaga tcccggaaaa ag

#atgaagcc    780

gcgcttgata cgctttataa aacaaacttt ggcgatatta ctactgctca gt

#taatgtcc    840

ccaagttatc tggcccggta ttatggcgtc tcaccggaag atattgccta cg

#tgacgact    900

tcattatcac atgttggata tagcagtgat attctggtta ttccgttggt cg

#atggtgtg    960

ggtaagatgg aagtagttcg tgttacccga acaccatcgg ataattatac ca

#gtcagacg   1020

aattatattg agctgtatcc acagggtggc gacaattatt tgatcaaata ca

#atctaagc   1080

aatagttttg gtttggatga tttttatctg caatataaag atggttccgc tg

#attggact   1140

gagattgccc ataatcccta tcctgatatg gtcataaatc aaaagtatga at

#cacaggcg   1200

acaatcaaac gtagtgactc tgacaatata ctcagtatag ggttacaaag at

#ggcatagc   1260

ggtagttata attttgccgc cgccaatttt aaaattgacc aatactcccc ga

#aagctttc   1320

ctgcttaaaa tgaataaggc tattcggttg ctcaaagcta ccggcctctc tt

#ttgctacg   1380

ttggagcgta ttgttgatag tgttaatagc accaaatcca tcacggttga gg

#tattaaac   1440

aaggtttatc gggtaaaatt ctatattgat cgttatggca tcagtgaaga ga

#cagccgct   1500

attttggcta atattaatat ctctcagcaa gctgttggca atcagcttag cc

#agtttgag   1560

caactattta atcacccgcc gctcaatggt attcgctatg aaatcagtga gg

#acaactcc   1620

aaacatcttc ctaatcctga tctgaacctt aaaccagaca gtaccggtga tg

#atcaacgc   1680

aaggcggttt taaaacgcgc gtttcaggtt aacgccagtg agttgtatca ga

#tgttattg   1740

atcactgatc gtaaagaaga cggtgttatc aaaaataact tagagaattt gt

#ctgatctg   1800

tatttggtta gtttgctggc ccagattcat aacctgacta ttgctgaatt ga

#acattttg   1860

ttggtgattt gtggctatgg cgacaccaac atttatcaga ttaccgacga ta

#atttagcc   1920

aaaatagtgg aaacattgtt gtggatcact caatggttga agacccaaaa at

#ggacagtt   1980

accgacctgt ttctgatgac cacggccact tacagcacca ctttaacgcc ag

#aaattagc   2040

aatctgacgg ctacgttgtc ttcaactttg catggcaaag agagtctgat tg

#gggaagat   2100

ctgaaaagag caatggcgcc ttgcttcact tcggctttgc atttgacttc tc

#aagaagtt   2160

gcgtatgacc tgctgttgtg gatagaccag attcaaccgg cacaaataac tg

#ttgatggg   2220

ttttgggaag aagtgcaaac aacaccaacc agcttgaagg tgattacctt tg

#ctcaggtg   2280

ctggcacaat tgagcctgat ctatcgtcgt attgggttaa gtgaaacgga ac

#tgtcactg   2340

atcgtgactc aatcttctct gctagtggca ggcaaaagca tactggatca cg

#gtctgtta   2400

accctgatgg ccttggaagg ttttcatacc tgggttaatg gcttggggca ac

#atgcctcc   2460

ttgatattgg cggcgttgaa agacggagcc ttgacagtta ccgatgtagc ac

#aagctatg   2520

aataaggagg aatctctcct acaaatggca gctaatcagg tggagaagga tc

#taacaaaa   2580

ctgaccagtt ggacacagat tgacgctatt ctgcaatggt tacagatgtc tt

#cggccttg   2640

gcggtttctc cactggatct ggcagggatg atggccctga aatatgggat ag

#atcataac   2700

tatgctgcct ggcaagctgc ggcggctgcg ctgatggctg atcatgctaa tc

#aggcacag   2760

aaaaaactgg atgagacgtt cagtaaggca ttatgtaact attatattaa tg

#ctgttgtc   2820

gatagtgctg ctggagtacg tgatcgtaac ggtttatata cctatttgct ga

#ttgataat   2880

caggtttctg ccgatgtgat cacttcacgt attgcagaag ctatcgccgg ta

#ttcaactg   2940

tacgttaacc gggctttaaa ccgagatgaa ggtcagcttg catcggacgt ta

#gtacccgt   3000

cagttcttca ctgactggga acgttacaat aaacgttaca gtacttgggc tg

#gtgtctct   3060

gaactggtct attatccaga aaactatgtt gatcccactc agcgcattgg gc

#aaaccaaa   3120

atgatggatg cgctgttgca atccatcaac cagagccagc taaatgcgga ta

#cggtggaa   3180

gatgctttca aaacttattt gaccagcttt gagcaggtag caaatctgaa ag

#taattagt   3240

gcttaccacg ataatgtgaa tgtggatcaa ggattaactt attttatcgg ta

#tcgaccaa   3300

gcagctccgg gtacgtatta ctggcgtagt gttgatcaca gcaaatgtga aa

#atggcaag   3360

tttgccgcta atgcttgggg tgagtggaat aaaattacct gtgctgtcaa tc

#cttggaaa   3420

aatatcatcc gtccggttgt ttatatgtcc cgcttatatc tgctatggct gg

#agcagcaa   3480

tcaaagaaaa gtgatgatgg taaaaccacg atttatcaat ataacttaaa ac

#tggctcat   3540

attcgttacg acggtagttg gaatacacca tttacttttg atgtgacaga aa

#aggtaaaa   3600

aattacacgt cgagtactga tgctgctgaa tctttagggt tgtattgtac tg

#gttatcaa   3660

ggggaagaca ctctattagt tatgttctat tcgatgcaga gtagttatag ct

#cctatacc   3720

gataataatg cgccggtcac tgggctatat attttcgctg atatgtcatc ag

#acaatatg   3780

acgaatgcac aagcaactaa ctattggaat aacagttatc cgcaatttga ta

#ctgtgatg   3840

gcagatccgg atagcgacaa taaaaaagtc ataaccagaa gagttaataa cc

#gttatgcg   3900

gaggattatg aaattccttc ctctgtgaca agtaacagta attattcttg gg

#gtgatcac   3960

agtttaacca tgctttatgg tggtagtgtt cctaatatta cttttgaatc gg

#cggcagaa   4020

gatttaaggc tatctaccaa tatggcattg agtattattc ataatggata tg

#cgggaacc   4080

cgccgtatac aatgtaatct tatgaaacaa tacgcttcat taggtgataa at

#ttataatt   4140

tatgattcat catttgatga tgcaaaccgt tttaatctgg tgccattgtt ta

#aattcgga   4200

aaagacgaga actcagatga tagtatttgt atatataatg aaaacccttc ct

#ctgaagat   4260

aagaagtggt atttttcttc gaaagatgac aataaaacag cggattataa tg

#gtggaact   4320

caatgtatag atgctggaac cagtaacaaa gatttttatt ataatctcca gg

#agattgaa   4380

gtaattagtg ttactggtgg gtattggtcg agttataaaa tatccaaccc ga

#ttaatatc   4440

aatacgggca ttgatagtgc taaagtaaaa gtcaccgtaa aagcgggtgg tg

#acgatcaa   4500

atctttactg ctgataatag tacctatgtt cctcagcaac cggcacccag tt

#ttgaggag   4560

atgatttatc agttcaataa cctgacaata gattgtaaga atttaaattt ca

#tcgacaat   4620

caggcacata ttgagattga tttcaccgct acggcacaag atggccgatt ct

#tgggtgca   4680

gaaactttta ttatcccggt aactaaaaaa gttctcggta ctgagaacgt ga

#ttgcgtta   4740

tatagcgaaa ataacggtgt tcaatatatg caaattggcg catatcgtac cc

#gtttgaat   4800

acgttattcg ctcaacagtt ggttagccgt gctaatcgtg gcattgatgc ag

#tgctcagt   4860

atggaaactc agaatattca ggaaccgcaa ttaggagcgg gcacatatgt gc

#agcttgtg   4920

ttggataaat atgatgagtc tattcatggc actaataaaa gctttgctat tg

#aatatgtt   4980

gatatattta aagagaacga tagttttgtg atttatcaag gagaacttag cg

#aaacaagt   5040

caaactgttg tgaaagtttt cttatcctat tttatagagg cgactggaaa ta

#agaaccac   5100

ttatgggtac gtgctaaata ccaaaaggaa acgactgata agatcttgtt cg

#accgtact   5160

gatgagaaag atccgcacgg ttggtttctc agcgacgatc acaagacctt ta

#gtggtctc   5220

tcttccgcac aggcattaaa gaacgacagt gaaccgatgg atttctctgg cg

#ccaatgct   5280

ctctatttct gggaactgtt ctattacacg ccgatgatga tggctcatcg tt

#tgttgcag   5340

gaacagaatt ttgatgcggc gaaccattgg ttccgttatg tctggagtcc at

#ccggttat   5400

atcgttgatg gtaaaattgc tatctaccac tggaacgtgc gaccgctgga ag

#aagacacc   5460

agttggaatg cacaacaact ggactccacc gatccagatg ctgtagccca ag

#atgatccg   5520

atgcactaca aggtggctac ctttatggcg acgttggatc tgctaatggc cc

#gtggtgat   5580

gctgcttacc gccagttaga gcgtgatacg ttggctgaag ctaaaatgtg gt

#atacacag   5640

gcgcttaatc tgttgggtga tgagccacaa gtgatgctga gtacgacttg gg

#ctaatcca   5700

acattgggta atgctgcttc aaaaaccaca cagcaggttc gtcagcaagt gc

#ttacccag   5760

ttgcgtctca atagcagggt aaaaaccccg ttgctaggaa cagccaattc cc

#tgaccgct   5820

ttattcctgc cgcaggaaaa tagcaagctc aaaggctact ggcggacact gg

#cgcagcgt   5880

atgtttaatt tacgtcataa tctgtcgatt gacggccagc cgctctcctt gc

#cgctgtat   5940

gctaaaccgg ctgatccaaa agctttactg agtgcggcgg tttcagcttc tc

#aaggggga   6000

gccgacttgc cgaaggcgcc gctgactatt caccgcttcc ctcaaatgct ag

#aaggggca   6060

cggggcttgg ttaaccagct tatacagttc ggtagttcac tattggggta ca

#gtgagcgt   6120

caggatgcgg aagctatgag tcaactactg caaacccaag ccagcgagtt aa

#tactgacc   6180

agtattcgta tgcaggataa ccaattggca gagctggatt cggaaaaaac cg

#ccttgcaa   6240

gtctctttag ctggagtgca acaacggttt gacagctata gccaactgta tg

#aggagaac   6300

atcaacgcag gtgagcagcg agcgctggcg ttacgctcag aatctgctat tg

#agtctcag   6360

ggagcgcaga tttcccgtat ggcaggcgcg ggtgttgata tggcaccaaa ta

#tcttcggc   6420

ctggctgatg gcggcatgca ttatggtgct attgcctatg ccatcgctga cg

#gtattgag   6480

ttgagtgctt ctgccaagat ggttgatgcg gagaaagttg ctcagtcgga aa

#tatatcgc   6540

cgtcgccgtc aagaatggaa aattcagcgt gacaacgcac aagcggagat ta

#accagtta   6600

aacgcgcaac tggaatcact gtctattcgc cgtgaagccg ctgaaatgca aa

#aagagtac   6660

ctgaaaaccc agcaagctca ggcgcaggca caacttactt tcttaagaag ca

#aattcagt   6720

aatcaagcgt tatatagttg gttacgaggg cgtttgtcag gtatttattt cc

#agttctat   6780

gacttggccg tatcacgttg cctgatggca gagcaatcct atcaatggga ag

#ctaatgat   6840

aattccatta gctttgtcaa accgggtgca tggcaaggaa cttacgccgg ct

#tattgtgt   6900

ggagaagctt tgatacaaaa tctggcacaa atggaagagg catatctgaa at

#gggaatct   6960

cgcgctttgg aagtagaacg cacggtttca ttggcagtgg tttatgattc ac

#tggaaggt   7020

aatgatcgtt ttaatttagc ggaacaaata cctgcattat tggataaggg gg

#agggaaca   7080

gcaggaacta aagaaaatgg gttatcattg gctaatgcta tcctgtcagc tt

#cggtcaaa   7140

ttgtccgact tgaaactggg aacggattat ccagacagta tcgttggtag ca

#acaaggtt   7200

cgtcgtatta agcaaatcag tgtttcgcta cctgcattgg ttgggcctta tc

#aggatgtt   7260

caggctatgc tcagctatgg tggcagtact caattgccga aaggttgttc ag

#cgttggct   7320

gtgtctcatg gtaccaatga tagtggtcag ttccagttgg atttcaatga cg

#gcaaatac   7380

ctgccatttg aaggtattgc tcttgatgat cagggtacac tgaatcttca at

#ttccgaat   7440

gctaccgaca agcagaaagc aatattgcaa actatgagcg atattatttt gc

#atattcgt   7500

tataccatcc gt

#

#

#     7512

<210> SEQ ID NO 8

<211> LENGTH: 2504

<212> TYPE: PRT

<213> ORGANISM: Photorhabdus luminescens

<400> SEQUENCE: 8

Met Gln Asn Ser Leu Ser Ser Thr Ile Asp Th

#r Ile Cys Gln Lys Leu

1               5

#                10

#                15

Gln Leu Thr Cys Pro Ala Glu Ile Ala Leu Ty

#r Pro Phe Asp Thr Phe

20

#            25

#            30

Arg Glu Lys Thr Arg Gly Met Val Asn Trp Gl

#y Glu Ala Lys Arg Ile

35

#        40

#        45

Tyr Glu Ile Ala Gln Ala Glu Gln Asp Arg As

#n Leu Leu His Glu Lys

50

#    55

#    60

Arg Ile Phe Ala Tyr Ala Asn Pro Leu Leu Ly

#s Asn Ala Val Arg Leu

65

#70

#75

#80

Gly Thr Arg Gln Met Leu Gly Phe Ile Gln Gl

#y Tyr Ser Asp Leu Phe

85

#                90

#                95

Gly Asn Arg Ala Asp Asn Tyr Ala Ala Pro Gl

#y Ser Val Ala Ser Met

100

#           105

#           110

Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Ty

#r Arg Glu Ala Lys Asn

115

#       120

#       125

Leu His Asp Ser Ser Ser Ile Tyr Tyr Leu As

#p Lys Arg Arg Pro Asp

130

#   135

#   140

Leu Ala Ser Leu Met Leu Ser Gln Lys Asn Me

#t Asp Glu Glu Ile Ser

145                 1

#50                 1

#55                 1

#60

Thr Leu Ala Leu Ser Asn Glu Leu Cys Leu Al

#a Gly Ile Glu Thr Lys

165

#               170

#               175

Thr Gly Lys Ser Gln Asp Glu Val Met Asp Me

#t Leu Ser Thr Tyr Arg

180

#           185

#           190

Leu Ser Gly Glu Thr Pro Tyr His His Ala Ty

#r Glu Thr Val Arg Glu

195

#       200

#       205

Ile Val His Glu Arg Asp Pro Gly Phe Arg Hi

#s Leu Ser Gln Ala Pro

210

#   215

#   220

Ile Val Ala Ala Lys Leu Asp Pro Val Thr Le

#u Leu Gly Ile Ser Ser

225                 2

#30                 2

#35                 2

#40

His Ile Ser Pro Glu Leu Tyr Asn Leu Leu Il

#e Glu Glu Ile Pro Glu

245

#               250

#               255

Lys Asp Glu Ala Ala Leu Asp Thr Leu Tyr Ly

#s Thr Asn Phe Gly Asp

260

#           265

#           270

Ile Thr Thr Ala Gln Leu Met Ser Pro Ser Ty

#r Leu Ala Arg Tyr Tyr

275

#       280

#       285

Gly Val Ser Pro Glu Asp Ile Ala Tyr Val Th

#r Thr Ser Leu Ser His

290

#   295

#   300

Val Gly Tyr Ser Ser Asp Ile Leu Val Ile Pr

#o Leu Val Asp Gly Val

305                 3

#10                 3

#15                 3

#20

Gly Lys Met Glu Val Val Arg Val Thr Arg Th

#r Pro Ser Asp Asn Tyr

325

#               330

#               335

Thr Ser Gln Thr Asn Tyr Ile Glu Leu Tyr Pr

#o Gln Gly Gly Asp Asn

340

#           345

#           350

Tyr Leu Ile Lys Tyr Asn Leu Ser Asn Ser Ph

#e Gly Leu Asp Asp Phe

355

#       360

#       365

Tyr Leu Gln Tyr Lys Asp Gly Ser Ala Asp Tr

#p Thr Glu Ile Ala His

370

#   375

#   380

Asn Pro Tyr Pro Asp Met Val Ile Asn Gln Ly

#s Tyr Glu Ser Gln Ala

385                 3

#90                 3

#95                 4

#00

Thr Ile Lys Arg Ser Asp Ser Asp Asn Ile Le

#u Ser Ile Gly Leu Gln

405

#               410

#               415

Arg Trp His Ser Gly Ser Tyr Asn Phe Ala Al

#a Ala Asn Phe Lys Ile

420

#           425

#           430

Asp Gln Tyr Ser Pro Lys Ala Phe Leu Leu Ly

#s Met Asn Lys Ala Ile

435

#       440

#       445

Arg Leu Leu Lys Ala Thr Gly Leu Ser Phe Al

#a Thr Leu Glu Arg Ile

450

#   455

#   460

Val Asp Ser Val Asn Ser Thr Lys Ser Ile Th

#r Val Glu Val Leu Asn

465                 4

#70                 4

#75                 4

#80

Lys Val Tyr Arg Val Lys Phe Tyr Ile Asp Ar

#g Tyr Gly Ile Ser Glu

485

#               490

#               495

Glu Thr Ala Ala Ile Leu Ala Asn Ile Asn Il

#e Ser Gln Gln Ala Val

500

#           505

#           510

Gly Asn Gln Leu Ser Gln Phe Glu Gln Leu Ph

#e Asn His Pro Pro Leu

515

#       520

#       525

Asn Gly Ile Arg Tyr Glu Ile Ser Glu Asp As

#n Ser Lys His Leu Pro

530

#   535

#   540

Asn Pro Asp Leu Asn Leu Lys Pro Asp Ser Th

#r Gly Asp Asp Gln Arg

545                 5

#50                 5

#55                 5

#60

Lys Ala Val Leu Lys Arg Ala Phe Gln Val As

#n Ala Ser Glu Leu Tyr

565

#               570

#               575

Gln Met Leu Leu Ile Thr Asp Arg Lys Glu As

#p Gly Val Ile Lys Asn

580

#           585

#           590

Asn Leu Glu Asn Leu Ser Asp Leu Tyr Leu Va

#l Ser Leu Leu Ala Gln

595

#       600

#       605

Ile His Asn Leu Thr Ile Ala Glu Leu Asn Il

#e Leu Leu Val Ile Cys

610

#   615

#   620

Gly Tyr Gly Asp Thr Asn Ile Tyr Gln Ile Th

#r Asp Asp Asn Leu Ala

625                 6

#30                 6

#35                 6

#40

Lys Ile Val Glu Thr Leu Leu Trp Ile Thr Gl

#n Trp Leu Lys Thr Gln

645

#               650

#               655

Lys Trp Thr Val Thr Asp Leu Phe Leu Met Th

#r Thr Ala Thr Tyr Ser

660

#           665

#           670

Thr Thr Leu Thr Pro Glu Ile Ser Asn Leu Th

#r Ala Thr Leu Ser Ser

675

#       680

#       685

Thr Leu His Gly Lys Glu Ser Leu Ile Gly Gl

#u Asp Leu Lys Arg Ala

690

#   695

#   700

Met Ala Pro Cys Phe Thr Ser Ala Leu His Le

#u Thr Ser Gln Glu Val

705                 7

#10                 7

#15                 7

#20

Ala Tyr Asp Leu Leu Leu Trp Ile Asp Gln Il

#e Gln Pro Ala Gln Ile

725

#               730

#               735

Thr Val Asp Gly Phe Trp Glu Glu Val Gln Th

#r Thr Pro Thr Ser Leu

740

#           745

#           750

Lys Val Ile Thr Phe Ala Gln Val Leu Ala Gl

#n Leu Ser Leu Ile Tyr

755

#       760

#       765

Arg Arg Ile Gly Leu Ser Glu Thr Glu Leu Se

#r Leu Ile Val Thr Gln

770

#   775

#   780

Ser Ser Leu Leu Val Ala Gly Lys Ser Ile Le

#u Asp His Gly Leu Leu

785                 7

#90                 7

#95                 8

#00

Thr Leu Met Ala Leu Glu Gly Phe His Thr Tr

#p Val Asn Gly Leu Gly

805

#               810

#               815

Gln His Ala Ser Leu Ile Leu Ala Ala Leu Ly

#s Asp Gly Ala Leu Thr

820

#           825

#           830

Val Thr Asp Val Ala Gln Ala Met Asn Lys Gl

#u Glu Ser Leu Leu Gln

835

#       840

#       845

Met Ala Ala Asn Gln Val Glu Lys Asp Leu Th

#r Lys Leu Thr Ser Trp

850

#   855

#   860

Thr Gln Ile Asp Ala Ile Leu Gln Trp Leu Gl

#n Met Ser Ser Ala Leu

865                 8

#70                 8

#75                 8

#80

Ala Val Ser Pro Leu Asp Leu Ala Gly Met Me

#t Ala Leu Lys Tyr Gly

885

#               890

#               895

Ile Asp His Asn Tyr Ala Ala Trp Gln Ala Al

#a Ala Ala Ala Leu Met

900

#           905

#           910

Ala Asp His Ala Asn Gln Ala Gln Lys Lys Le

#u Asp Glu Thr Phe Ser

915

#       920

#       925

Lys Ala Leu Cys Asn Tyr Tyr Ile Asn Ala Va

#l Val Asp Ser Ala Ala

930

#   935

#   940

Gly Val Arg Asp Arg Asn Gly Leu Tyr Thr Ty

#r Leu Leu Ile Asp Asn

945                 9

#50                 9

#55                 9

#60

Gln Val Ser Ala Asp Val Ile Thr Ser Arg Il

#e Ala Glu Ala Ile Ala

965

#               970

#               975

Gly Ile Gln Leu Tyr Val Asn Arg Ala Leu As

#n Arg Asp Glu Gly Gln

980

#           985

#           990

Leu Ala Ser Asp Val Ser Thr Arg  Gln Phe

#Phe Thr Asp  Trp Glu Arg

995

#       1000

#       1005

Tyr Asn  Lys Arg Tyr Ser Thr  Trp Ala G

#ly Val Ser  Glu Leu Val

1010

#    1015

#    1020

Tyr Tyr  Pro Glu Asn Tyr Val  Asp Pro T

#hr Gln Arg  Ile Gly Gln

1025

#    1030

#    1035

Thr Lys  Met Met Asp Ala Leu  Leu Gln S

#er Ile Asn  Gln Ser Gln

1040

#    1045

#    1050

Leu Asn  Ala Asp Thr Val Glu  Asp Ala P

#he Lys Thr  Tyr Leu Thr

1055

#    1060

#    1065

Ser Phe  Glu Gln Val Ala Asn  Leu Lys V

#al Ile Ser  Ala Tyr His

1070

#    1075

#    1080

Asp Asn  Val Asn Val Asp Gln  Gly Leu T

#hr Tyr Phe  Ile Gly Ile

1085

#    1090

#    1095

Asp Gln  Ala Ala Pro Gly Thr  Tyr Tyr T

#rp Arg Ser  Val Asp His

1100

#    1105

#    1110

Ser Lys  Cys Glu Asn Gly Lys  Phe Ala A

#la Asn Ala  Trp Gly Glu

1115

#    1120

#    1125

Trp Asn  Lys Ile Thr Cys Ala  Val Asn P

#ro Trp Lys  Asn Ile Ile

1130

#    1135

#    1140

Arg Pro  Val Val Tyr Met Ser  Arg Leu T

#yr Leu Leu  Trp Leu Glu

1145

#    1150

#    1155

Gln Gln  Ser Lys Lys Ser Asp  Asp Gly L

#ys Thr Thr  Ile Tyr Gln

1160

#    1165

#    1170

Tyr Asn  Leu Lys Leu Ala His  Ile Arg T

#yr Asp Gly  Ser Trp Asn

1175

#    1180

#    1185

Thr Pro  Phe Thr Phe Asp Val  Thr Glu L

#ys Val Lys  Asn Tyr Thr

1190

#    1195

#    1200

Ser Ser  Thr Asp Ala Ala Glu  Ser Leu G

#ly Leu Tyr  Cys Thr Gly

1205

#    1210

#    1215

Tyr Gln  Gly Glu Asp Thr Leu  Leu Val M

#et Phe Tyr  Ser Met Gln

1220

#    1225

#    1230

Ser Ser  Tyr Ser Ser Tyr Thr  Asp Asn A

#sn Ala Pro  Val Thr Gly

1235

#    1240

#    1245

Leu Tyr  Ile Phe Ala Asp Met  Ser Ser A

#sp Asn Met  Thr Asn Ala

1250

#    1255

#    1260

Gln Ala  Thr Asn Tyr Trp Asn  Asn Ser T

#yr Pro Gln  Phe Asp Thr

1265

#    1270

#    1275

Val Met  Ala Asp Pro Asp Ser  Asp Asn L

#ys Lys Val  Ile Thr Arg

1280

#    1285

#    1290

Arg Val  Asn Asn Arg Tyr Ala  Glu Asp T

#yr Glu Ile  Pro Ser Ser

1295

#    1300

#    1305

Val Thr  Ser Asn Ser Asn Tyr  Ser Trp G

#ly Asp His  Ser Leu Thr

1310

#    1315

#    1320

Met Leu  Tyr Gly Gly Ser Val  Pro Asn I

#le Thr Phe  Glu Ser Ala

1325

#    1330

#    1335

Ala Glu  Asp Leu Arg Leu Ser  Thr Asn M

#et Ala Leu  Ser Ile Ile

1340

#    1345

#    1350

His Asn  Gly Tyr Ala Gly Thr  Arg Arg I

#le Gln Cys  Asn Leu Met

1355

#    1360

#    1365

Lys Gln  Tyr Ala Ser Leu Gly  Asp Lys P

#he Ile Ile  Tyr Asp Ser

1370

#    1375

#    1380

Ser Phe  Asp Asp Ala Asn Arg  Phe Asn L

#eu Val Pro  Leu Phe Lys

1385

#    1390

#    1395

Phe Gly  Lys Asp Glu Asn Ser  Asp Asp S

#er Ile Cys  Ile Tyr Asn

1400

#    1405

#    1410

Glu Asn  Pro Ser Ser Glu Asp  Lys Lys T

#rp Tyr Phe  Ser Ser Lys

1415

#    1420

#    1425

Asp Asp  Asn Lys Thr Ala Asp  Tyr Asn G

#ly Gly Thr  Gln Cys Ile

1430

#    1435

#    1440

Asp Ala  Gly Thr Ser Asn Lys  Asp Phe T

#yr Tyr Asn  Leu Gln Glu

1445

#    1450

#    1455

Ile Glu  Val Ile Ser Val Thr  Gly Gly T

#yr Trp Ser  Ser Tyr Lys

1460

#    1465

#    1470

Ile Ser  Asn Pro Ile Asn Ile  Asn Thr G

#ly Ile Asp  Ser Ala Lys

1475

#    1480

#    1485

Val Lys  Val Thr Val Lys Ala  Gly Gly A

#sp Asp Gln  Ile Phe Thr

1490

#    1495

#    1500

Ala Asp  Asn Ser Thr Tyr Val  Pro Gln G

#ln Pro Ala  Pro Ser Phe

1505

#    1510

#    1515

Glu Glu  Met Ile Tyr Gln Phe  Asn Asn L

#eu Thr Ile  Asp Cys Lys

1520

#    1525

#    1530

Asn Leu  Asn Phe Ile Asp Asn  Gln Ala H

#is Ile Glu  Ile Asp Phe

1535

#    1540

#    1545

Thr Ala  Thr Ala Gln Asp Gly  Arg Phe L

#eu Gly Ala  Glu Thr Phe

1550

#    1555

#    1560

Ile Ile  Pro Val Thr Lys Lys  Val Leu G

#ly Thr Glu  Asn Val Ile

1565

#    1570

#    1575

Ala Leu  Tyr Ser Glu Asn Asn  Gly Val G

#ln Tyr Met  Gln Ile Gly

1580

#    1585

#    1590

Ala Tyr  Arg Thr Arg Leu Asn  Thr Leu P

#he Ala Gln  Gln Leu Val

1595

#    1600

#    1605

Ser Arg  Ala Asn Arg Gly Ile  Asp Ala V

#al Leu Ser  Met Glu Thr

1610

#    1615

#    1620

Gln Asn  Ile Gln Glu Pro Gln  Leu Gly A

#la Gly Thr  Tyr Val Gln

1625

#    1630

#    1635

Leu Val  Leu Asp Lys Tyr Asp  Glu Ser I

#le His Gly  Thr Asn Lys

1640

#    1645

#    1650

Ser Phe  Ala Ile Glu Tyr Val  Asp Ile P

#he Lys Glu  Asn Asp Ser

1655

#    1660

#    1665

Phe Val  Ile Tyr Gln Gly Glu  Leu Ser G

#lu Thr Ser  Gln Thr Val

1670

#    1675

#    1680

Val Lys  Val Phe Leu Ser Tyr  Phe Ile G

#lu Ala Thr  Gly Asn Lys

1685

#    1690

#    1695

Asn His  Leu Trp Val Arg Ala  Lys Tyr G

#ln Lys Glu  Thr Thr Asp

1700

#    1705

#    1710

Lys Ile  Leu Phe Asp Arg Thr  Asp Glu L

#ys Asp Pro  His Gly Trp

1715

#    1720

#    1725

Phe Leu  Ser Asp Asp His Lys  Thr Phe S

#er Gly Leu  Ser Ser Ala

1730

#    1735

#    1740

Gln Ala  Leu Lys Asn Asp Ser  Glu Pro M

#et Asp Phe  Ser Gly Ala

1745

#    1750

#    1755

Asn Ala  Leu Tyr Phe Trp Glu  Leu Phe T

#yr Tyr Thr  Pro Met Met

1760

#    1765

#    1770

Met Ala  His Arg Leu Leu Gln  Glu Gln A

#sn Phe Asp  Ala Ala Asn

1775

#    1780

#    1785

His Trp  Phe Arg Tyr Val Trp  Ser Pro S

#er Gly Tyr  Ile Val Asp

1790

#    1795

#    1800

Gly Lys  Ile Ala Ile Tyr His  Trp Asn V

#al Arg Pro  Leu Glu Glu

1805

#    1810

#    1815

Asp Thr  Ser Trp Asn Ala Gln  Gln Leu A

#sp Ser Thr  Asp Pro Asp

1820

#    1825

#    1830

Ala Val  Ala Gln Asp Asp Pro  Met His T

#yr Lys Val  Ala Thr Phe

1835

#    1840

#    1845

Met Ala  Thr Leu Asp Leu Leu  Met Ala A

#rg Gly Asp  Ala Ala Tyr

1850

#    1855

#    1860

Arg Gln  Leu Glu Arg Asp Thr  Leu Ala G

#lu Ala Lys  Met Trp Tyr

1865

#    1870

#    1875

Thr Gln  Ala Leu Asn Leu Leu  Gly Asp G

#lu Pro Gln  Val Met Leu

1880

#    1885

#    1890

Ser Thr  Thr Trp Ala Asn Pro  Thr Leu G

#ly Asn Ala  Ala Ser Lys

1895

#    1900

#    1905

Thr Thr  Gln Gln Val Arg Gln  Gln Val L

#eu Thr Gln  Leu Arg Leu

1910

#    1915

#    1920

Asn Ser  Arg Val Lys Thr Pro  Leu Leu G

#ly Thr Ala  Asn Ser Leu

1925

#    1930

#    1935

Thr Ala  Leu Phe Leu Pro Gln  Glu Asn S

#er Lys Leu  Lys Gly Tyr

1940

#    1945

#    1950

Trp Arg  Thr Leu Ala Gln Arg  Met Phe A

#sn Leu Arg  His Asn Leu

1955

#    1960

#    1965

Ser Ile  Asp Gly Gln Pro Leu  Ser Leu P

#ro Leu Tyr  Ala Lys Pro

1970

#    1975

#    1980

Ala Asp  Pro Lys Ala Leu Leu  Ser Ala A

#la Val Ser  Ala Ser Gln

1985

#    1990

#    1995

Gly Gly  Ala Asp Leu Pro Lys  Ala Pro L

#eu Thr Ile  His Arg Phe

2000

#    2005

#    2010

Pro Gln  Met Leu Glu Gly Ala  Arg Gly L

#eu Val Asn  Gln Leu Ile

2015

#    2020

#    2025

Gln Phe  Gly Ser Ser Leu Leu  Gly Tyr S

#er Glu Arg  Gln Asp Ala

2030

#    2035

#    2040

Glu Ala  Met Ser Gln Leu Leu  Gln Thr G

#ln Ala Ser  Glu Leu Ile

2045

#    2050

#    2055

Leu Thr  Ser Ile Arg Met Gln  Asp Asn G

#ln Leu Ala  Glu Leu Asp

2060

#    2065

#    2070

Ser Glu  Lys Thr Ala Leu Gln  Val Ser L

#eu Ala Gly  Val Gln Gln

2075

#    2080

#    2085

Arg Phe  Asp Ser Tyr Ser Gln  Leu Tyr G

#lu Glu Asn  Ile Asn Ala

2090

#    2095

#    2100

Gly Glu  Gln Arg Ala Leu Ala  Leu Arg S

#er Glu Ser  Ala Ile Glu

2105

#    2110

#    2115

Ser Gln  Gly Ala Gln Ile Ser  Arg Met A

#la Gly Ala  Gly Val Asp

2120

#    2125

#    2130

Met Ala  Pro Asn Ile Phe Gly  Leu Ala A

#sp Gly Gly  Met His Tyr

2135

#    2140

#    2145

Gly Ala  Ile Ala Tyr Ala Ile  Ala Asp G

#ly Ile Glu  Leu Ser Ala

2150

#    2155

#    2160

Ser Ala  Lys Met Val Asp Ala  Glu Lys V

#al Ala Gln  Ser Glu Ile

2165

#    2170

#    2175

Tyr Arg  Arg Arg Arg Gln Glu  Trp Lys I

#le Gln Arg  Asp Asn Ala

2180

#    2185

#    2190

Gln Ala  Glu Ile Asn Gln Leu  Asn Ala G

#ln Leu Glu  Ser Leu Ser

2195

#    2200

#    2205

Ile Arg  Arg Glu Ala Ala Glu  Met Gln L

#ys Glu Tyr  Leu Lys Thr

2210

#    2215

#    2220

Gln Gln  Ala Gln Ala Gln Ala  Gln Leu T

#hr Phe Leu  Arg Ser Lys

2225

#    2230

#    2235

Phe Ser  Asn Gln Ala Leu Tyr  Ser Trp L

#eu Arg Gly  Arg Leu Ser

2240

#    2245

#    2250

Gly Ile  Tyr Phe Gln Phe Tyr  Asp Leu A

#la Val Ser  Arg Cys Leu

2255

#    2260

#    2265

Met Ala  Glu Gln Ser Tyr Gln  Trp Glu A

#la Asn Asp  Asn Ser Ile

2270

#    2275

#    2280

Ser Phe  Val Lys Pro Gly Ala  Trp Gln G

#ly Thr Tyr  Ala Gly Leu

2285

#    2290

#    2295

Leu Cys  Gly Glu Ala Leu Ile  Gln Asn L

#eu Ala Gln  Met Glu Glu

2300

#    2305

#    2310

Ala Tyr  Leu Lys Trp Glu Ser  Arg Ala L

#eu Glu Val  Glu Arg Thr

2315

#    2320

#    2325

Val Ser  Leu Ala Val Val Tyr  Asp Ser L

#eu Glu Gly  Asn Asp Arg

2330

#    2335

#    2340

Phe Asn  Leu Ala Glu Gln Ile  Pro Ala L

#eu Leu Asp  Lys Gly Glu

2345

#    2350

#    2355

Gly Thr  Ala Gly Thr Lys Glu  Asn Gly L

#eu Ser Leu  Ala Asn Ala

2360

#    2365

#    2370

Ile Leu  Ser Ala Ser Val Lys  Leu Ser A

#sp Leu Lys  Leu Gly Thr

2375

#    2380

#    2385

Asp Tyr  Pro Asp Ser Ile Val  Gly Ser A

#sn Lys Val  Arg Arg Ile

2390

#    2395

#    2400

Lys Gln  Ile Ser Val Ser Leu  Pro Ala L

#eu Val Gly  Pro Tyr Gln

2405

#    2410

#    2415

Asp Val  Gln Ala Met Leu Ser  Tyr Gly G

#ly Ser Thr  Gln Leu Pro

2420

#    2425

#    2430

Lys Gly  Cys Ser Ala Leu Ala  Val Ser H

#is Gly Thr  Asn Asp Ser

2435

#    2440

#    2445

Gly Gln  Phe Gln Leu Asp Phe  Asn Asp G

#ly Lys Tyr  Leu Pro Phe

2450

#    2455

#    2460

Glu Gly  Ile Ala Leu Asp Asp  Gln Gly T

#hr Leu Asn  Leu Gln Phe

2465

#    2470

#    2475

Pro Asn  Ala Thr Asp Lys Gln  Lys Ala I

#le Leu Gln  Thr Met Ser

2480

#    2485

#    2490

Asp Ile  Ile Leu His Ile Arg  Tyr Thr I

#le Arg

2495

#    2500