USPatent applicationPatented

Plant metabolism genes

Granted 13 Jan 2004 · 2 office actions

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9614912
filed 12 Jul 2000
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Not published
not published
Patent
US 6,677,502
granted 13 Jan 2004

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Abstract

This invention relates to an isolated nucleic acid fragment encoding a GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase protein. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the protein in a transformed host cell.

Description

81 parts
›This application claims the benefit of U.S. Provisional…

This application claims the benefit of U.S. Provisional Applications No. 60/143,401 filed Jul. 12, 1999; No. 60/143,412, filed Jul. 12, 1999; No. 60/146,650, filed Jul. 30, 1999; No. 60/170,906 filed Dec. 15, 1999; No. 60/172,959 filed Dec. 21, 1999; No. 60/172,946 filed Dec. 21, 1999.

›FIELD OF THE INVENTION

This invention is in the field of plant molecular biology. More specifically, this invention pertains to nucleic acid fragments encoding metabolism proteins in plants and seeds.

›BACKGROUND OF THE INVENTION · 1 of 4

Gibberellic acid (GA) is an important regulator (phytohormone) of plant development. Gibberellic acid has been shown to stimulate elongation in the internodes of stems and to play roles in flower and fruit development. Identification and characterization of genes involved in GA biosynthesis will permit genetic engineering methods aimed at modulating levels of GA in plants which will in turn allow for better control of plant stature, fertility and plant development in general (see World Patent Publication No. WO 95/35383).

Gibberellic acid is synthesized from isoprenoid geranylgeranyl diphosphate (GGDP), beginning with the conversion of GGDP to copalyl diphosphate (CDP). Copalyl diphosphate is then converted to GA 12 -aldehyde which in turn can be converted to a number of different gibberellins required for normal plant development. For example GA-20 oxidase catalizes the conversion of GA12 to GA9. A key enzyme in the synthesis of gibberellin is dioxygenase which appears to play a role in the conversion of GA 12 -aldehyde to GA 9 and GA 25 . Because GA 12 -aldehyde dioxygenase appears to catalyze key steps in the synthesis of GA it is a target enzymes that may be manipulated to control GA levels. Ent-Kaurene synthase A (KSA) catalyzes the conversion of GGDP to CDP, which is subsequently converted to ent-kaurene by ent-kaurene synthase B (KSB) (Yamaguchi et al. (1996) Plant J. 10(2):203-213). Gibberillin 3-beta-hydroxylase catalizes the conversion of GA20 to GA1 a major gibberellin that is involved in controlling stem elongation. These enzymes catalyze key steps in the synthesis of GA and thus provide target enzymes that may be manipulated to control GA levels.

Thus there is a great deal of interest in identifying genes that encode proteins that may be used to control plant developmental. Accordingly, the availability of nucleic acid sequences encoding all or a substantial portion of a GA dioxygenase would facilitate studies to better understand plant development and provide tools to genetically engineer improved developmental properties in plants.

Riboflavin is the precursor to essential electron transport chain components and redox coenzymes such as flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Humans, unlike plants and bacteria, are incapable of synthesizing riboflavin (vitamin B 2 ) from GTP, and must obtain this compound through their diet. Thousands of tons of riboflavin are produced each year as additives for food and animal feed (Bacher et al. (1997) Methods Enzymol 280:382-389). Historically, riboflavin has been made via chemical synthesis, however recent advances in biotechnology have enabled industrial production using yeast and bacteria (Humbelin et al. (1999) J Indust Micro & Biotech 22:1-7). The biologically synthesized riboflavin is cheaper to produce, and the process is better for the environment.

Several enzymatic steps are required to take GTP to riboflavin. The first step in bacteria is catalyzed by a GTP cyclohydrolase II activity which takes GTP to 2,5-iamino-6-ribosylamino-4(3H)-pyrimidinone 5′-phosphate. The enzyme performing this step is encoded by the ribA gene. RibA has two enzymatic activities, the above mentioned cyclohydrolase and a 3,4-dihydroxy-2-butanone 4-phosphate synthase activity that takes ribulose 5-phosphate to L-3,4-dihydroxy-2-butanone-4-phosphate, which is combined to a pathway intermediate, to form 6,7-dimethyl-8-ribityllumazine, the penultimate intermediate to riboflavin. The second step in the pathway is encoded by ribG a riboflavin-specific deaminase.

Studies using riboflavin over-producing Bacillus subtilis strains, have led to the conclusion that the GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase enzyme is rate-limiting for high-level riboflavin accumulation (Humbelin et al. (1999) J Indust Micro & Biotech 22:1-7). Increasing the copy number of the ribA gene in these strains results in improved riboflavin productivity. The pathways leading to riboflavin biosynthesis are largely conserved between plants and bacteria. Therefore, the potential exists for improving the riboflavin content in crop plants, thus reducing the need for vitamin supplementation in food.

The present invention describes the identification and utility of GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase sequences from corn, rice, soybean, and wheat. Also disclosed are sequences from corn, rice, and wheat that encode riboflavin-specific deaminases. It is believed that modulation of these activities through over-expression, under-expression, or mutation will lead to altered levels of riboflavin in plants.

Hormones in animal systems and phytohormones in plants control many metabolic processes. Phytohormones differ in their structure and specific actions compared to animal hormones, though the signal transduction mechanisms involved may be similar in plants and animals. Phytohormones affect shoot elongation, stem elongation, root growth, seed dormancy, fruit ripening, leaf senescence and morphogenesis, disease resistance (Hoffman et al. (1999) Plant Physiol 119:935-949), to name a few.

Among the phytohormones that have been studied so far, ethylene is the simplest in terms of chemical structure. Its effects, however, are far-ranging, affecting seed dormancy, fruit ripening and abscission, flower development, leaf senescence, adventitious root formation, and shoot and root growth and differentiation. Ethylene is synthesized from methionine via three enzymatic steps. Methionine is converted to S-adenosyl-methionine (SAM) by SAM synthetase, which is then converted to 1-aminocyclopropane-1-carboxylic acid (ACC) by ACC synthase. Finally, ACC oxidase acts on ACC to yield ethylene. The genes encoding these enzymes have been cloned, and transgenic approaches based on these genes have been attempted to control ethylene levels, and consequently fruit ripening. Using antisense technology, ACC synthase or ACC oxidase activities have been reduced in transgenic plants leading to inhibition of fruit ripening (Hamilton et al. (1990) Nature 346:284-287; Oeller et al. (1991) Science 254:437-439).

›BACKGROUND OF THE INVENTION · 2 of 4

The mechanisms by which ethylene regulates plant development however are yet to be clearly defined. Ethylene-insensitive mutants and constitutive ethylene response mutants, principally in Arabidopsis, have been valuable in outlining the ethylene response pathway (Kieber (1997) Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:277-296). The isolation of the genes affected in these mutants indicates the involvement of a protein kinase cascade in ethylene signaling. CTR1, a negative regulator of the ethylene response encodes a serine/threonine kinase that is most similar to the Raf family of protein kinases (Kieber et al. (1993) Cell 72:427-441). ETR1 in which dominant mutations lead to defective ethylene responses encodes a protein that is similar to bacterial two-component histidine kinases (Chang et al. (1993) Science 262:539-544). It most probably serves as an ethylene receptor/ethylene response factor since etr1 mutant seedlings bind ethylene at reduced levels compared to wild-type (Bleecker et al. (1988) Science 241:1086-1089), the ETR1 protein has been shown to bind ethylene (Schaller and Bleeker (1995) Science 270:1809-1811), and genetic epistasis analysis puts ETR1 the start of the ethylene response pathway (Kieber et al. (1993) Cell 72:427-441). U.S. Pat. Nos. 5,689,055 and 5,824,868 describe the Arabidopsis ETR1 gene, its tomato homologs, and their use in generating transgenic plants with modified response to ethylene.

ETR1 belongs to a small gene family in Arabidopsis, and at least one ETR1 homolog in Arabidopsis, ERS, has already been cloned (Hua et al. (1995) Science 269:1712-1714). Homologs in other species like rice and tomato have been isolated as well (Wilkinson et al. (1995) Science 270:1807-1809; Yau and Yip (1997) Plant Physiol 115:315; Tieman and Klee (1999) Plant Physiol 120:165-172). Many questions however remain regarding ethylene response factor gene organization, evolution, structure and function. Accordingly, additional nucleic acid sequences encoding ethylene response factors are disclosed herein which would facilitate studies to better understand ethylene response factors and ethylene signaling in plants and could provide genetic tools to enhance or otherwise alter developmental and physiological processes regulated by ethylene.

Acyl-CoA thioesterases catalyze the hydrolysis of variable length acyl-CoAs to produce free fatty acids and CoASH. Acyl-CoA thioesterase activities are generally found in most organisms from prokaryotes to eukaryotes. Eukaryotic acyl-CoA thioesterase activities have been detected in various subcellular organelles including lysosomes, peroxisomes, and mitochondria, as well as in the cytosol. Long chain acyl-CoA esters are important intermediates in degradation and synthesis of fatty acids, and may have important roles in regulating intermediary metabolism and gene expression (Waku (1992) Biochem Biophys Acta 1124:101-111). In animal systems, free fatty acids and acyl-CoAs have been shown to be nuclear receptor ligands which regulate lipid homeostasis. Also, acyl-CoAs act as potent feedback inhibitors of fatty acid synthesis. Typically, fatty acids entering cells are rapidly esterified to their corresponding CoA-esters. These acyl-CoAs are then oxidized in mitochondria or peroxisomes, elongated, desaturated or esterified into complex lipids or transferred post-translationally to proteins. The precise role of acyl-CoA thioesterases, their substrates, and products is not yet fully understood. However it is clear that animal and yeast acyl-CoA thioesterase enzymes are involved in the regulation of lipid metabolism by modulation of cellular concentrations of acyl-CoAs and fatty acids. It is unclear at this time what role the acyl-CoA thioesterases play in plants although these enzymatic activities are well known in plants (Murphy et al. (1984) Eur J Biochem 142:43-48).

Previously, acyl-CoA thioesterases were thought to coordinate with the fatty acid synthases in the biosynthesis of fatty acids in the cytosol. Although this is true, recent findings of peroxisomal and mitochondrial forms of the enzyme implicate additional roles for the thioesterases since peroxisomes and mitochondria do not contain synthase activities (Hunt et al. (1999) J Biol Chem 274:34317-34326, Jones et al. (1999) J Biol Chem 274:9216-9223, and Liu et al. (1997) J Biol Chem 272:13779-13785). Many aspects of fatty acid utilization and regulation are tied into growth rates and the ability of plants to adapt to their environment. It is therefore likely that the acyl-CoA thioesterases will play some role in regulating plant metabolism, just as they do in animal systems.

Members of the superfamily of adenosine triphosphate (ATP)-binding-cassette (ABC) transport systems couple the hydrolysis of ATP to the translocation of solutes across a biological membrane. Recognized by their common modular organization and two sequence motifs that constitute a nucleotide binding fold, ABC transporters are widespread among all living organisms. They accomplish not only the uptake of nutrients in bacteria but are involved in diverse processes, such as signal transduction, protein secretion, drug and antibiotic resistance, antigen presentation, bacterial pathogenesis and sporulation. Moreover, some human inheritable diseases, like cystic fibrosis, adrenoleukodystrophy and Stargardt's disease are caused by defective ABC transport systems. Details of the molecular mechanism by which these systems exert their functions are still poorly understood (Schneider, E. and Hunke, S. (1998) FEMS Microbiol Rev 22:1-20).

The pleiotropic drug resistance gene PDR5 encodes a protein which is a member of the ABC-transport protein superfamily. This ABC transporter functions as a drug extrusion pump by being involved in the ATP-dependent efflux of a variety of structurally unrelated cytotoxic compounds. The transcription regulators PDR1, PDR3, PDR7, and PDR9 control the expression of the gene PDR5 (Balzi, E. and Goffeau, A. (1995) J Bioenerg Biomembr 27:71-76). PDR5 encodes a 160-kDa protein with a predicted duplicated six membrane-spanning domain and a repeated putative ATP-binding domain. PDR5 shares this structural feature with the mammalian multidrug resistance pumps as well as the functional capacity of conferring resistance to various inhibitors upon amplification (Leppert, G. et al. (1990) Genetics 125:13-20). PDR5 homologs are present in plants, may function during stress conditions in an analogous fashion to that described in yeast, and expression of such ABC transporters is subject to a complex hormonal and environmental regulation (Smart, C. C. and Fleming, A. J. (1996) J Biol Chem 271:19351-19357).

›BACKGROUND OF THE INVENTION · 3 of 4

Also a member of the ABC-superfamily, GCN20 uptakes ions and amino acids. GCN20 is co-immunoprecipitated from cell extracts with GCN 1, another factor required to activate GCN2, and the two proteins interact in the yeast two-hybrid system. These two factors indicate that GCN1 and GCN20 are components of a protein complex that couples the kinase activity of GCN2 to the availability of amino acids. GCN20 is closely related to ABC proteins identified in Caenorhabditis elegans, rice and humans, suggesting that the function of GCN20 may be conserved among diverse eukaryotic organisms (Vazquez de Aldana, C. R. et al. (1995) EMBO J 14:3184-3199). As part of the GCN1/GCN20 complex, GCN20 may be involved in the modulation of the EF3-related function which facilitates the activation of GCN2 by uncharged tRNA on translating ribosomes (Marton, M. J. et al. (1997) Mol Cell Biol 17:4474-4489).

ABC transporters play a role in the protection of organisms against exogenous toxins by cellular detoxification processes. P-glycoprotein, the product of the multidrug resistance (MDR1) gene, is an ATP-driven transmembrane pump that increases the resistance of cells by actively exporting toxic chemicals. In addition to transporting anticancer drugs, P-glycoprotein also extrudes steroid hormones and a variety of lipophilic drugs, such as calcium channel blockers, phenothiazines, cyclosporines, etc.

The 70-kDa peroxisomal membrane protein (PMP70) is one of the major integral membrane proteins of rat liver peroxisomes. The carboxyl-terminal region of PMP70 has strong sequence similarities to a group of ATP-binding proteins such as MalK and MDR. These proteins form a superfamily and are involved in various biological processes including membrane transport. The PMP70 protein is a transmembrane protein possibly forming a channel, with its ATP-binding domain exposed to the cytosol. PMP70 is involved in active transport across the peroxisomal membrane. (Kamijo, K. et al. (1990) J. Biol. Chem. 265:4534-4540).

Immunoblot analysis shows that PMP70 is associated with the peroxisomal membrane in liver, renal cortex, and jejunal mucosa (Usuda, N. et al. (1991) Cytochem 39:1357-1366). cDNAs for human and rat PMP70 have been cloned and sequenced and the gene mapped to human chromosome 1p21-22. In humans, mutations in the PMP70 gene have been found in a subset of patients with Zellweger-syndrome, a lethal inborn error of peroxisome biogenesis (Gartner, J. and Valle, D. (1993) i Semin Cell Biol 4:45-52). Members of an MDR-like gene family, with no similarity to PMP70, have been identified from an Arabidopsis thaliana cDNA library (Dudler, R. and Hertig, C. (1991) J. Biol. Chem. 267:5882-5888). No other plant MDR-like genes have been identified to date, although identification of these genes in plants will be useful to understand the herbicide resistance.

Plants produce cytotoxic compounds to which they are susceptible, and are exposed to exogenous toxins (microbial products, allelochemicals, and agrochemicals) making cell survival contingent on mechanisms for detoxifying these agents. One detoxification mechanism is the glutathione S-transferase-catalyzed glutathionation of the toxin, or an activated derivative, and transport of the conjugate out of the cytosol. The glutathione S-conjugate (GS-X) pump family is a new class of ATP-binding cassette (ABC) transporters responsible for the elimination and/or sequestration of pharmacologically and agronomically important compounds in mammalian, yeast and plant cells. The molecular structure and function of GS-X pumps encoded by mammalian and plant MRP, cMOAT (canalicular multispecific anion transporter), and YCF1 (yeast cadmium factor) genes have been conserved throughout molecular evolution. The physiologic function of GS-X pumps is closely related to cellular detoxification, oxidative stress, inflammation, and cancer drug resistance. Coordinated expression of GS-X pump genes, such as MRP1 and YCF1, and gamma-glutamylcystaine synthetase, a rate-limiting enzyme of cellular glutathione biosynthesis, is frequently observed (Ishikawa T. et al. (1997) Biosci. Rep. 17:189-207).

Four expressed sequence tags identified from an Arabidopsis cDNA library have deduced amino acid sequences which are highly similar to MRP and YCF1. These genes are differentially expressed in response to treatments of Arabidopsis with several herbicides, heavy metals and other toxic compounds indicating the involvement of different ABC transporters in different-detoxification mechanisms. The full-length cDNA from one of these ESTs (named AtMRP) has been sequenced and this information is present in GenBank (Accession No. U92650). At the amino acid level AtMRP is 34% identical to both MRP and YCF1 but its biochemical function has not been demonstrated (Tommasini, R. et al. (1997) FEBS Lett. 411:206-210).

An AtMRP1 gene which encodes a transporter responsible for the removal of glutathione S-conjugates from the cytosol has been isolated and sequenced. The AtMRP1 gene encodes an ABC transporter competent in the transport of glutathione S-conjugates of xenobiotics and endogenous substances, including herbicides and anthocyanins (Lu, Y. P. et al. (1997) Proc Natl Acad Sci USA 94:8243-8248). AtMRP1 possesses the same overall domain organization as HmMRP1 and ScYCF1. These three ABC transporters catalyze Mg-ATP-energized, vanadate-inhibitable transport of GS conjugates. A cDNA encoding a putative ABC transporter from Arabidopsis, AtMRP1 (not necessarily the same one as above), was identified based on sequence similarities to mammalian MRP1 (Marin, E. et al. (1998) Biochim Biophys Acta 1369:7-13).

Another gene, AtMRP2, from Arabidopsis has been isolated which is 87% identical at the amino acid level to AtMRP1 described by Lu et al. (1997) Proc Natl Acad Sci USA 94:8243-8248. This gene belongs to the same subclass of ABC transporter genes as AtMRP1, and its heterologous expression in yeast also confers Mg-ATP-energized GS conjugate transport. Besides transporting glutathione conjugates, AtMRP2 transports at high capacity Bn-NCC-1, a malonyl ester of the predominant nonfluorescent chlorophyll catabolite from Brassica napus. AtMRP2 simultaneously transports Bn-NCC-1 and GS conjugates without either substrate interfering with the transport of the other suggesting that the same pump may have both functions (Lu, Y. P. (1998) Plant Cell 10:267-282).

›BACKGROUND OF THE INVENTION · 4 of 4

AtMRP3, another ABC-transporter from Arabidopsis thaliana exhibits high sequence similarity to the human (MRP1) and yeast (YCF1) glutathione-conjugate transporters and complements a cadmium-sensitive yeast mutant that also lacks glutathione-conjugate transport activity. The kinetic properties of AtMRP3 are very similar to those described for the vacuolar glutathione-conjugate transporter of barley and mung bean. AtMRP3 also is involved in the uptake of the chlorophyll catabolite Bn-NCC-1 (Tommasini, R. et al. (1998) Plant J. 13:773-780).

Also forming part of the Arabidopsis thaliana MRP gene family, encoding a putative GS-X pump, is AtMRP4. The derived amino acid sequence from AtMRP4 shares high levels of similarity (55-63%) with human, yeast, and other Arabidopsis homologues. Expression of the different members of the AtMRP gene family in Arabidopsis cell suspensions after treatment with chemicals that modify glutathione metabolism (compounds that induce different types of stress and that act as herbicide antidotes- safenus- in monocotyledonous species) reveals that the members of this gene family are differentially regulated (Sanchez-Fernandez, R. (1998) Mol. Gen. Genet: 258:655-662).

›SUMMARY OF THE INVENTION · 1 of 4

An isolated polynucleotide comprising a first nucleotide sequence selected from the group consisting of: (a) first nucleotide sequence encoding a polypeptide of at least 30 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202 and 204; or (b) a second nucleotide sequence comprising a complement of the first nucleotide sequence.

The present invention concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 351 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, and 8, 50, 82, 100 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 72 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:12, 16, 18, 20, 22, 24, 26 and 28, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 110 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:10, 32, 36, 38, 40, 42, 44, 46, 48, 52, 60, 102, 104, 124 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 189 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:14, 34, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 120, 122 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 67 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:84, 86, 88, 90, 92, 94 and 96, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 87 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:98, 110, 112, 114, 116 and 118, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 155 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:128, 130, 132, 134, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 161 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:54, 136, 138, 140, 142 144, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 66 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166 168 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 141 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:58, 106, 170, 172, 174, 176, 178, 180, 182 and 184 or (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 81 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:186, 188, 190, 192, 194, 196, 198, 200, 202, 204 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

›SUMMARY OF THE INVENTION · 2 of 4

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of (a) a first nucleotide sequence encoding a polypeptide of at least 48 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:30, 56, 108, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

In a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:1, 3, 5, 7, 49, 81 and 99 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, 8, 50, 82 and 100.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a first nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:11, 15, 17, 19, 21, 23, 25 and 27 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:12, 16, 18, 20, 22, 24, 26 and 28.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:9, 31, 35, 37, 39, 41, 43, 45, 47, 51, 59, 101, 103 and 123 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:10, 32, 36, 38, 40, 42, 44, 46, 48, 52, 60, 102, 104, and 124.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:13, 33, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 119 and 121 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:14, 34, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 120 and 122.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:83, 85, 87, 89, 91, 93 and 95 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:84, 86, 88, 90, 92, 94 and 96.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:97, 109, 111, 113, 115 and 117 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:98, 110, 112, 114, 116 and 118.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:127, 129, 131 and 133 that codes for the polypeptide selected from the group consisting of SEQ ID NOs: 128, 130, 132 and 134.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:53, 135, 137, 139, 141 and 143 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:54, 136, 138, 140, 142 and 144.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165 and 167 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166 and 168.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:57, 105, 169, 171, 173, 175, 177, 179, 181 and 183 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:58, 106, 170, 172, 174, 176, 178, 180, 182 and 184.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the claimed invention comprises a nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:185, 187, 189, 191, 193, 195, 197, 199, 201 and 203 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:186, 188, 190, 192, 194, 196, 198, 200, 202 and 204.

Also in a second embodiment, it is preferred that the isolated polynucleotide of the invention comprises a first nucleotide sequence which comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:29, 55 and 107, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:30, 56 and 108.

In a third embodiment, this invention concerns an isolated polynucleotide comprising a nucleotide sequence of at least one of 60 (preferably at least one of 40, most preferably at least one of 30) contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183 185, 187, 189, 191, 193, 195, 197, 199, 201 and 203 and the complement of such nucleotide sequences.

In a fourth embodiment, this invention relates to a chimeric gene comprising an isolated polynucleotide of the present invention operably linked to at least one suitable regulatory sequence.

›SUMMARY OF THE INVENTION · 3 of 4

In a fifth embodiment, the present invention concerns a host cell comprising a chimeric gene of the present invention or an isolated polynucleotide of the present invention. The host cell may be eukaryotic, such as a yeast or a plant cell, or prokaryotic, such as a bacterial cell. The present invention also relates to a virus, preferably a baculovirus, comprising an isolated polynucleotide of the present invention or a chimeric gene of the present invention.

In a sixth embodiment, the invention also relates to a process for producing a host cell comprising a chimeric gene of the present invention or an isolated polynucleotide of the present invention, the process comprising either transforming or transfecting a compatible host cell with a chimeric gene or isolated polynucleotide of the present invention.

In a seventh embodiment, the invention concerns a polypeptide of at least 351 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, 8, 50, 82, and 100.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 72 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:12, 16, 18, 20, 22, 24, 26 and 28.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 110 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:10, 32, 36, 38, 40, 42, 44, 46, 48, 52, 60, 102, 104 and 124.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 189 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:14, 34, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 120 and 122.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 67 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:84, 86, 88, 90, 92, 94 and 96.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 87 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:98, 110, 112, 114, 116 and 118.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 155 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs: 128, 130, 132 and 134.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 161 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:54, 136, 138, 140, 142 and 144.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 66 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166 and 168.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 141 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:58, 106, 170, 172, 174, 176, 178, 180, 182 and 184.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 81 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:186, 188, 190, 192, 194, 196, 198, 200, 202 and 204.

Also in a seventh embodiment, the invention concerns a polypeptide of at least 48 amino acids comprising at least 80% identity based on the Clustal method of alignment compared to a polypeptide selected from the group consisting of SEQ ID NOs:30, 56 and 108.

In an eighth embodiment, the invention relates to a method of selecting an isolated polynucleotide that affects the level of expression of a dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide or enzyme activity in a host cell, preferably a plant cell, the method comprising the steps of: (a) constructing an isolated polynucleotide of the present invention or a chimeric gene of the present invention; (b) introducing the isolated polynucleotide or the chimeric gene into a host cell; (c) measuring the level of the dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide or enzyme activity in the host cell containing the isolated polynucleotide; and (d) comparing the level of the dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide or enzyme activity in the host cell containing the isolated polynucleotide with the level of the dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide or enzyme activity in the host cell that does not contain the isolated polynucleotide.

›SUMMARY OF THE INVENTION · 4 of 4

In a ninth embodiment, the invention concerns a method of obtaining a nucleic acid fragment encoding a substantial portion of a dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide, preferably a plant dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide, comprising the steps of: synthesizing an oligonucleotide primer comprising a nucleotide sequence of at least one of 60 (preferably at least one of 40, most preferably at least one of 30) contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ ID: NOs: dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter, and the complement of such nucleotide sequences; and amplifying a nucleic acid fragment (preferably a cDNA inserted in a cloning vector) using the oligonucleotide primer. The amplified nucleic acid fragment preferably will encode a substantial portion of a dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter amino acid sequence.

In a tenth embodiment, this invention relates to a method of obtaining a nucleic acid fragment encoding all or a substantial portion of the amino acid sequence encoding a dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide comprising the steps of: probing a cDNA or genomic library with an isolated polynucleotide of the present invention; identifying a DNA clone that hybridizes with an isolated polynucleotide of the present invention; isolating the identified DNA clone; and sequencing the cDNA or genomic fragment that comprises the isolated DNA clone.

In an eleventh embodiment, this invention concerns a composition, such as a hybridization mixture, comprising an isolated polynucleotide or polypeptide of the present invention.

In a twelfth embodiment, this invention concerns a method for positive selection of a transformed cell comprising: (a) transforming a host cell with the chimeric gene of the present invention or a construct of the present invention; and (b) growing the transformed host cell, preferably a plant cell, such as a monocot or a dicot, under conditions which allow expression of the dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polynucleotide in an amount sufficient to complement a null mutant to provide a positive selection means.

In a thirteenth embodiment, this invention relates to a method of altering the level of expression of a metabolism protein in a host cell comprising: (a) transforming a host cell with a chimeric gene of the present invention; and (b) growing the transformed host cell under conditions that are suitable for expression of the chimeric gene wherein expression of the chimeric gene results in production of altered levels of the metabolism proteins in the transformed host cell.

›BRIEF DESCRIPTION OF THE SEQUENCE LISTINGS

The invention can be more fully understood from the following detailed description and the accompanying Sequence Listing which form a part of this application.

Table 1 lists the polypeptides that are described herein, the designation of the cDNA clones that comprise the nucleic acid fragments encoding polypeptides representing all or a substantial portion of these polypeptides, and the corresponding identifier (SEQ ID NO:) as used in the attached Sequence Listing. The sequence descriptions and Sequence Listing attached hereto comply with the rules governing nucleotide and/or amino acid sequence disclosures in patent applications as set forth in 37 C.F.R. §1.821-1.825. Table 1 also identifies the cDNA clones as individual ESTs (“EST”), the sequences of the entire cDNA inserts comprising the indicated cDNA clones (“FIS”), contigs assembled from two or more ESTs (“Contig”), contigs assembled from an FIS and one or more ESTs (“Contig*”), or sequences encoding the entire protein derived from an FIS, a contig, or an FIS and PCR (“CGS”).

Nucleotide sequences, SEQ ID NOs:1, 3, 5 and 7 and amino acid sequences SEQ ID NOs:2, 4, 6 and 8 were determined by further sequence analysis of cDNA clones encoding the amino acid sequences set forth in SEQ ID NOs:10, 12, 14 and 16. Nucleotide SEQ ID NOs:9, 11, 13 and 15 amino acid SEQ ID NOs:10, 12, 14 and 16 were presented in a U.S. Provisional Application No. 60/143,401, filed Jul. 12, 1999.

Nucleotide sequences, SEQ ID NOs:17, 21 and 23 and amino acid sequences SEQ ID NOs:18, 20 and 24 were determined by further sequence analysis of cDNA clones encoding the amino acid sequences set forth in SEQ ID NOs:30, 32 and 34. Nucleotide SEQ ID NOs:25, 27, 29, 31 and 33 amino acid SEQ ID NOs:26, 28, 30, 32 and 34 were presented in a U.S. Provisional Application No. 60/143,412, filed Jul. 12, 1999.

Nucleotide sequences, SEQ ID NOs:35, 37, 39, 41, 43 and 45 and amino acid sequences SEQ ID NOs:36, 38, 40, 42, 44 and 46 were determined by further sequence analysis of cDNA clones encoding the amino acid sequences set forth in SEQ ID NOs:50, 52, 54, 56, 58 and 60. Nucleotide SEQ ID NOs:47, 49, 51. 53. 55, 57 and 59 amino acid SEQ ID NOs:48, 50, 52, 54, 56, 58 and 60 were presented in a U.S. Provisional Application No. 60/146,650 filed Jul. 30, 1999.

Nucleotide sequence, SEQ ID NO:69 and amino acid sequence SEQ ID NO:70, were determined by further sequence analysis of cDNA clones encoding the amino acid sequence set forth in SEQ ID NO:82. Nucleotide SEQ ID NOs:65, 73 and 81 amino acid SEQ ID NOs:66, 74 and 82 were presented in a U.S. Provisional Application No. 60/170,906 filed Dec. 14, 1999.

Nucleotide sequences, SEQ ID NOs:85, 87, 89, 91, 93 and 95 and amino acid sequences SEQ ID NOs:84, 86, 88, 90, 92, 94 and 96, were determined by further sequence analysis of cDNA clones encoding the amino acid sequences set forth in SEQ ID NOs:100, 102, 104, 106, 108 and 110. Nucleotide SEQ ID NOs:97, 99, 101, 103, 105, 107 and 109 and amino acid SEQ ID NOs:98, 100, 102, 104, 106, 108 and 110 were presented in a U.S. Provisional Application No. 60/172,959 filed Dec. 21, 1999.

Nucleotide sequences, SEQ ID NOs:109, 111, 115 and 117 and amino acid sequences SEQ ID NOs:110, 112, 116 and 118, were determined by further sequence analysis of cDNA clones encoding the amino acid sequences set forth in SEQ ID NO:120, 122, 124 and 126. Nucleotide SEQ ID NOs:113, 119, 121, 123 and 125 amino acid SEQ ID NOs:114, 120, 122, 124 and 126 were presented in a U.S. Provisional Application No. 60/172,946 filed Dec. 21, 1999.

The Sequence Listing contains the one letter code for nucleotide sequence characters and the three letter codes for amino acids as defined in conformity with the IUPAC-IUBMB standards described in Nucleic Acids Res. 13:3021-3030 (1985) and in the Biochemical J. 219 (No. 2):345-373 (1984) which are herein incorporated by reference. The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. §1.822.

›DETAILED DESCRIPTION OF THE INVENTION · 1 of 9

In the context of this disclosure, a number of terms shall be utilized. The terms “polynucleotide”, “polynucleotide sequence”, “nucleic acid sequence”, and “nucleic acid fragment”/“isolated nucleic acid fragment” are used interchangeably herein. These terms encompass nucleotide sequences and the like. A polynucleotide may be a polymer of RNA or DNA that is single- or double-stranded, that optionally contains synthetic, non-natural or altered nucleotide bases. A polynucleotide in the form of a polymer of DNA may be comprised of one or more segments of cDNA, genomic DNA, synthetic DNA, or mixtures thereof. An isolated polynucleotide of the present invention may include at least one of 60 contiguous nucleotides, preferably at least one of 40 contiguous nucleotides, most preferably one of at least 30 contiguous nucleotides derived from SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183 185, 187, 189, 191, 193, 195, 197, 199, 201 and 203, or the complement of such sequences.

The term “isolated polynucleotide” refers to a polynucleotide that is substantially free from other nucleic acid sequences, such as and not limited to other chromosomal and extrachromosomal DNA and RNA, that normally accompany or interact with it as found in its naturally occuring environment. Isolated polynucleotides may be purified from a host cell in which they naturally occur. Conventional nucleic acid purification methods known to skilled artisans may be used to obtain isolated polynucleotides. The term also embraces recombinant polynucleotides and chemically synthesized polynucleotides.

The term “recombinant” means, for example, that a nucleic acid sequence is made by an artificial combination of two otherwise separated segments of sequence, e.g., by chemical synthesis or by the manipulation of isolated nucleic acids by genetic engineering techniques.

As used herein, “contig” refers to a nucleotide sequence that is assembled from two or more constituent nucleotide sequences that share common or overlapping regions of sequence homology. For example, the nucleotide sequences of two or more nucleic acid fragments can be compared and aligned in order to identify common or overlapping sequences. Where common or overlapping sequences exist between two or more nucleic acid fragments, the sequences (and thus their corresponding nucleic acid fragments) can be assembled into a single contiguous nucleotide sequence.

As used herein, “substantially similar” refers to nucleic acid fragments wherein changes in one or more nucleotide bases results in substitution of one or more amino acids, but do not affect the functional properties of the polypeptide encoded by the nucleotide sequence. “Substantially similar” also refers to nucleic acid fragments wherein changes in one or more nucleotide bases does not affect the ability of the nucleic acid fragment to mediate alteration of gene expression by gene silencing through for example antisense or co-suppression technology. “Substantially similar” also refers to modifications of the nucleic acid fragments of the instant invention such as deletion or insertion of one or more nucleotides that do not substantially affect the functional properties of the resulting transcript vis-a-vis the ability to mediate gene silencing or alteration of the functional properties of the resulting protein molecule. It is therefore understood that the invention encompasses more than the specific exemplary nucleotide or amino acid sequences and includes functional equivalents thereof. The terms “substantially similar” and “corresponding substantially” are used interchangeably herein.

Substantially similar nucleic acid fragments may be selected by screening nucleic acid fragments representing subfragments or modifications of the nucleic acid fragments of the instant invention, wherein one or more nucleotides are substituted, deleted and/or inserted, for their ability to affect the level of the polypeptide encoded by the unmodified nucleic acid fragment in a plant or plant cell. For example, a substantially similar nucleic acid fragment representing at least one of 30 contiguous nucleotides derived from the instant nucleic acid fragment can be constructed and introduced into a plant or plant cell. The level of the polypeptide encoded by the unmodified nucleic acid fragment present in a plant or plant cell exposed to the substantially similar nucleic fragment can then be compared to the level of the polypeptide in a plant or plant cell that is not exposed to the substantially similar nucleic acid fragment.

For example, it is well known in the art that antisense suppression and co-suppression of gene expression may be accomplished using nucleic acid fragments representing less than the entire coding region of a gene, and by using nucleic acid fragments that do not share 100% sequence identity with the gene to be suppressed. Moreover, alterations in a nucleic acid fragment which result in the production of a chemically equivalent amino acid at a given site, but do not effect the functional properties of the encoded polypeptide, are well known in the art. Thus, a codon for the amino acid alanine, a hydrophobic amino acid, may be substituted by a codon encoding another less hydrophobic residue, such as glycine, or a more hydrophobic residue, such as valine, leucine, or isoleucine. Similarly, changes which result in substitution of one negatively charged residue for another, such as aspartic acid for glutamic acid, or one positively charged residue for another, such as lysine for arginine, can also be expected to produce a functionally equivalent product. Nucleotide changes which result in alteration of the N-terminal and C-terminal portions of the polypeptide molecule would also not be expected to alter the activity of the polypeptide. Each of the proposed modifications is well within the routine skill in the art, as is determination of retention of biological activity of the encoded products. Consequently, an isolated polynucleotide comprising a nucleotide sequence of at least one of 60 (preferably at least one of 40, most preferably at least one of 30) contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183 185, 187, 189, 191, 193, 195, 197, 199, 201 and 203, and the complement of such nucleotide sequences may be used in methods of selecting an isolated polynucleotide that affects the expression of a dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide in a host cell. A method of selecting an isolated polynucleotide that affects the level of expression of a polypeptide in a virus or in a host cell (eukaryotic, such as plant or yeast, prokaryotic such as bacterial) may comprise the steps of: constructing an isolated polynucleotide of the present invention or a chimeric gene of the present invention; introducing the isolated polynucleotide or the chimeric gene into a host cell; measuring the level of a polypeptide or enzyme activity in the host cell containing the isolated polynucleotide; and comparing the level of a polypeptide or enzyme activity in the host cell containing the isolated polynucleotide with the level of a polypeptide or enzyme activity in a host cell that does not contain the isolated polynucleotide.

›DETAILED DESCRIPTION OF THE INVENTION · 2 of 9

Moreover, substantially similar nucleic acid fragments may also be characterized by their ability to hybridize. Estimates of such homology are provided by either DNA-DNA or DNA-RNA hybridization under conditions of stringency as is well understood by those skilled in the art (Hames and Higgins, Eds. (1985) Nucleic Acid Hybridisation, IRL Press, Oxford, U.K.). Stringency conditions can be adjusted to screen for moderately similar fragments, such as homologous sequences from distantly related organisms, to highly similar fragments, such as genes that duplicate functional enzymes from closely related organisms. Post-hybridization washes determine stringency conditions. One set of preferred conditions uses a series of washes starting with 6×SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2×SSC, 0.5% SDS at 45° C. for 30 min, and then repeated twice with 0.2×SSC, 0.5% SDS at 50° C. for 30 min. A more preferred set of stringent conditions uses higher temperatures in which the washes are identical to those above except for the temperature of the final two 30 min washes in 0.2×SSC, 0.5% SDS which was increased to 60° C. Another preferred set of highly stringent conditions uses two final washes in 0.1×SSC, 0.1% SDS at 65° C.

Substantially similar nucleic acid fragments of the instant invention may also be characterized by the percent identity of the amino acid sequences that they encode to the amino acid sequences disclosed herein, as determined by algorithms commonly employed by those skilled in this art. Suitable nucleic acid fragments (isolated polynucleotides of the present invention) encode polypeptides that are at least about 70% identical, preferably at least about 80% identical to the amino acid sequences reported herein. Preferred nucleic acid fragments encode amino acid sequences that are about 85% identical to the amino acid sequences reported herein. More preferred nucleic acid fragments encode amino acid sequences that are at least about 90% identical to the amino acid sequences reported herein. Most preferred are nucleic acid fragments that encode amino acid sequences that are at least about 95% identical to the amino acid sequences reported herein. Suitable nucleic acid fragments not only have the above identities but typically encode a polypeptide having at least 50 amino acids, preferably at least 100 amino acids, more preferably at least 150 amino acids, still more preferably at least 200 amino acids, and most preferably at least 250 amino acids. Sequence alignments and percent identity calculations were performed using the Megalign program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Multiple alignment of the sequences was performed using the Clustal method of alignment (Higgins and Sharp (1989) CABIOS. 5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments using the Clustal method were KTUPLE 1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5.

A “substantial portion” of an amino acid or nucleotide sequence comprises an amino acid or a nucleotide sequence that is sufficient to afford putative identification of the protein or gene that the amino acid or nucleotide sequence comprises. Amino acid and nucleotide sequences can be evaluated either manually by one skilled in the art, or by using computer-based sequence comparison and identification tools that employ algorithms such as BLAST (Basic Local Alignment Search Tool; Altschul et al. (1993) J. Mol. Biol. 215:403-410). In general, a sequence of ten or more contiguous amino acids or thirty or more contiguous nucleotides is necessary in order to putatively identify a polypeptide or nucleic acid sequence as homologous to a known protein or gene. Moreover, with respect to nucleotide sequences, gene-specific oligonucleotide probes comprising 30 or more contiguous nucleotides may be used in sequence-dependent methods of gene identification (e.g., Southern hybridization) and isolation (e.g., in situ hybridization of bacterial colonies or bacteriophage plaques). In addition, short oligonucleotides of 12 or more nucleotide, may be used as amplification primers in PCR in order to obtain a particular nucleic acid fragment comprising the primers. Accordingly, a “substantial portion” of a nucleotide sequence comprises a nucleotide sequence that will afford specific identification and/or isolation of a nucleic acid fragment comprising the sequence. The instant specification teaches amino acid and nucleotide sequences encoding polypeptides that comprise one or more particular plant proteins. The skilled artisan, having the benefit of the sequences as reported herein, may now use all or a substantial portion of the disclosed sequences for purposes known to those skilled in this art. Accordingly, the instant invention comprises the complete sequences as reported in the accompanying Sequence Listing, as well as substantial portions of those sequences as defined above.

“Codon degeneracy” refers to divergence in the genetic code permitting variation of the nucleotide sequence without effecting the amino acid sequence of an encoded polypeptide. Accordingly, the instant invention relates to any nucleic acid fragment comprising a nucleotide sequence that encodes all or a substantial portion of the amino acid sequences set forth herein. The skilled artisan is well aware of the “codon-bias” exhibited by a specific host cell in usage of nucleotide codons to specify a given amino acid. Therefore, when synthesizing a nucleic acid fragment for improved expression in a host cell, it is desirable to design the nucleic acid fragment such that its frequency of codon usage approaches the frequency of preferred codon usage of the host cell.

“Synthetic nucleic acid fragments” can be assembled from oligonucleotide building blocks that are chemically synthesized using procedures known to those skilled in the art. These building blocks are ligated and annealed to form larger nucleic acid fragments which may then be enzymatically assembled to construct the entire desired nucleic acid fragment. “Chemically synthesized”, as related to a nucleic acid fragment, means that the component nucleotides were assembled in vitro. Manual chemical synthesis of nucleic acid fragments may be accomplished using well established procedures, or automated chemical synthesis can be performed using one of a number of commercially available machines. Accordingly, the nucleic acid fragments can be tailored for optimal gene expression based on optimization of the nucleotide sequence to reflect the codon bias of the host cell. The skilled artisan appreciates the likelihood of successful gene expression if codon usage is biased towards those codons favored by the host. Determination of preferred codons can be based on a survey of genes derived from the host cell where sequence information is available.

›DETAILED DESCRIPTION OF THE INVENTION · 3 of 9

“Gene” refers to a nucleic acid fragment that expresses a specific protein, including regulatory sequences preceding (5′ non-coding sequences) and following (3′ non-coding sequences) the coding sequence. “Native gene” refers to a gene as found in nature with its own regulatory sequences. “Chimeric gene” refers any gene that is not a native gene, comprising regulatory and coding sequences that are not found together in nature. Accordingly, a chimeric gene may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. “Endogenous gene” refers to a native gene in its natural location in the genome of an organism. A “foreign gene” refers to a gene not normally found in the host organism, but that is introduced into the host organism by gene transfer. Foreign genes can comprise native genes inserted into a non-native organism, or chimeric genes. A “transgene” is a gene that has been introduced into the genome by a transformation procedure.

“Coding sequence” refers to a nucleotide sequence that codes for a specific amino acid sequence. “Regulatory sequences” refers to nucleotide sequences located upstream (5′ non-coding sequences), within, or downstream (3′ non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence. Regulatory sequences may include promoters, translation leader sequences, introns, and polyadenylation recognition sequences.

“Promoter” refers to a nucleotide sequence capable of controlling the expression of a coding sequence or functional RNA. In general, a coding sequence is located 3′ to a promoter sequence. The promoter sequence consists of proximal and more distal upstream elements, the latter elements often referred to as enhancers. Accordingly, an “enhancer” is a nucleotide sequence which can stimulate promoter activity and may be an innate element of the promoter or a heterologous element inserted to enhance the level or tissue-specificity of a promoter. Promoters may be derived in their entirety from a native gene, or may be composed of different elements derived from different promoters found in nature, or may even comprise synthetic nucleotide segments. It is understood by those skilled in the art that different promoters may direct the expression of a gene in different tissues or cell types, or at different stages of development, or in response to different environmental conditions. Promoters which cause a nucleic acid fragment to be expressed in most cell types at most times are commonly referred to as “constitutive promoters”. New promoters of various types useful in plant cells are constantly being discovered; numerous examples may be found in the compilation by Okamuro and Goldberg (1989) Biochemistry of Plants 15:1-82. It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, nucleic acid fragments of different lengths may have identical promoter activity.

“Translation leader sequence” refers to a nucleotide sequence located between the promoter sequence of a gene and the coding sequence. The translation leader sequence is present in the fully processed mRNA upstream of the translation start sequence. The translation leader sequence may affect processing of the primary transcript to mRNA, mRNA stability or translation efficiency. Examples of translation leader sequences have been described (Turner and Foster (1995) Mol. Biotechnol. 3:225-236).

“3′ Non-coding sequences” refers to nucleotide sequences located downstream of a coding sequence and includes polyadenylation recognition sequences and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3′ end of the mRNA precursor. The use of different 3 non-coding sequences is exemplified by Ingelbrecht et al. (1989) Plant Cell 1:671-680.

“RNA transcript” refers to the product resulting from RNA polymerase-catalyzed transcription of a DNA sequence. When the RNA transcript is a perfect complementary copy of the DNA sequence, it is referred to as the primary transcript or it may be a RNA sequence derived from posttranscriptional processing of the primary transcript and is referred to as the mature RNA. “Messenger RNA (mRNA)” refers to the RNA that is without introns and can be translated into polypeptides by the cell. “cDNA” refers to DNA that is complementary to and derived from an mRNA template. The cDNA can be single-stranded or converted to double stranded form using, for example, the Klenow fragment of DNA polymerase I. “Sense RNA” refers to an RNA transcript that includes the mRNA and can be translated into a polypeptide by the cell. “Antisense RNA” refers to an RNA transcript that is complementary to all or part of a target primary transcript or mRNA and that blocks the expression of a target gene (see U.S. Pat. No. 5,107,065, incorporated herein by reference). The complementarity of an antisense RNA may be with any part of the specific nucleotide sequence, i.e., at the 5′ non-coding sequence, 3′ non-coding sequence, introns, or the coding sequence. “Functional RNA” refers to sense RNA, antisense RNA, ribozyme RNA, or other RNA that may not be translated but yet has an effect on cellular processes.

The term “operably linked” refers to the association of two or more nucleic acid fragments so that the function of one is affected by the other. For example, a promoter is operably linked with a coding sequence when it is capable of affecting the expression of that coding sequence (i.e., that the coding sequence is under the transcriptional control of the promoter). Coding sequences can be operably linked to regulatory sequences in sense or antisense orientation.

›DETAILED DESCRIPTION OF THE INVENTION · 4 of 9

The term “expression”, as used herein, refers to the transcription and stable accumulation of sense (mRNA) or antisense RNA derived from the nucleic acid fragment of the invention. “Expression” may also refer to translation of mRNA into a polypeptide. “Antisense inhibition” refers to the production of antisense RNA transcripts capable of suppressing the expression of the target protein. “Overexpression” refers to the production of a gene product in transgenic organisms that exceeds levels of production in normal or non-transformed organisms. “Co-suppression” refers to the production of sense RNA transcripts capable of suppressing the expression of identical or substantially similar foreign or endogenous genes (U.S. Pat. No. 5,231,020, incorporated herein by reference).

A “protein” or “polypeptide” is a chain of amino acids arranged in a specific order determined by the coding sequence in a polynucleotide encoding the polypeptide. Each protein or polypeptide has a unique function.

“Altered levels” or “altered expression” refers to the production of gene product(s) in transgenic organisms in amounts or proportions that differ from that of normal or non-transformed organisms.

“Null mutant” refers to a host cell which either lacks the expression of a certain polypeptide or expresses a polypeptide which is inactive or does not have any detectable expected enzymatic function.

“Mature protein” or the term “mature” when used in describing a protein refers to a post-translationally processed polypeptide; i.e., one from which any pre- or propeptides present in the primary translation product have been removed. “Precursor protein” or the term “precursor” when used in describing a protein refers to the primary product of translation of mRNA; i.e., with pre- and propeptides still present. Pre- and propeptides may be but are not limited to intracellular localization signals.

A “chloroplast transit peptide” is an amino acid sequence which is translated in conjunction with a protein and directs the protein to the chloroplast or other plastid types present in the cell in which the protein is made. “Chloroplast transit sequence” refers to a nucleotide sequence that encodes a chloroplast transit peptide. A “signal peptide” is an amino acid sequence which is translated in conjunction with a protein and directs the protein to the secretory system (Chrispeels (1991) Ann. Rev. Plant Phys. Plant Mol. Biol. 42:21-53). If the protein is to be directed to a vacuole, a vacuolar targeting signal (supra) can further be added, or if to the endoplasmic reticulum, an endoplasmic reticulum retention signal (supra) may be added. If the protein is to be directed to the nucleus, any signal peptide present should be removed and instead a nuclear localization signal included (Raikhel (1992) Plant Phys. 100:1627-1632).

“Transformation” refers to the transfer of a nucleic acid fragment into the genome of a host organism, resulting in genetically stable inheritance. Host organisms containing the transformed nucleic acid fragments are referred to as “transgenic” organisms. Examples of methods of plant transformation include Agrobacterium-mediated transformation (De Blaere et al. (1987) Meth. Enzymol. 143:277) and particle-accelerated or “gene gun” transformation technology (Klein et al. (1987) Nature (London) 327:70-73; U.S. Pat. No. 4,945,050, incorporated herein by reference). Thus, isolated polynucleotides of the present invention can be incorporated into recombinant constructs, typically DNA constructs, capable of introduction into and replication in a host cell. Such a construct can be a vector that includes a replication system and sequences that are capable of transcription and translation of a polypeptide-encoding sequence in a given host cell. A number of vectors suitable for stable transfection of plant cells or for the establishment of transgenic plants have been described in, e.g., Pouwels et al., Cloning Vectors: A Laboratory Manual, 1985, supp. 1987; Weissbach and Weissbach, Methods for Plant Molecular Biology, Academic Press, 1989; and Flevin et al., Plant Molecular Biology Manual, Kluwer Academic Publishers, 1990. Typically, plant expression vectors include, for example, one or more cloned plant genes under the transcriptional control of 5′ and 3′ regulatory sequences and a dominant selectable marker. Such plant expression vectors also can contain a promoter regulatory region (e.g., a regulatory region controlling inducible or constitutive, environmentally- or developmentally-regulated, or cell- or tissue-specific expression), a transcription initiation start site, a ribosome binding site, an RNA processing signal, a transcription termination site, and/or a polyadenylation signal.

Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described more fully in Sambrook et al. Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, 1989 (hereinafter “Maniatis”).

“PCR” or “polymerase chain reaction” is well known by those skilled in the art as a technique used for the amplification of specific DNA segments (U.S. Pat. Nos. 4,683,195 and 4,800,159).

The present invention concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) first nucleotide sequence encoding a polypeptide of at least 351 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, 8, 50, 82 and 100 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) first nucleotide sequence encoding a polypeptide of at least 72 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:12, 16, 18, 20, 22, 24, 26 and 28, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

›DETAILED DESCRIPTION OF THE INVENTION · 5 of 9

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) first nucleotide sequence encoding a polypeptide of at least 110 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:10, 32, 36, 38, 40, 42, 44, 46, 48, 52, 60, 102, 104, 124or (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 189 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:14, 34, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 120, 122 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 67 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:84, 86, 88, 90, 92, 94 and 96, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 87 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:98, 110, 112, 114, 116 and 118, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 155 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:128, 130, 132, and 134 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 161 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:54, 136, 138, 140, 142 and 144 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 66 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs: 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166 and 168 and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 141 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:58, 106, 170, 172, 174, 176, 178, 180, 182 and 184, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 81 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:186, 188, 190, 192, 194, 196, 198, 200, 202 and 204, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

The present invention also concerns an isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (a) a first nucleotide sequence encoding a polypeptide of at least 48 amino acids having at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:30, 56 and 108, and (b) a second nucleotide sequence comprising the complement of the first nucleotide sequence.

Preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:1, 3, 5, 7, 49, 81 and 99 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, 8, 50, 82 and 100.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:11, 15, 17, 19, 21, 23, 25 and 27, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:12, 16, 18, 20, 22, 24, 26 and 28.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:9, 31, 35, 37, 39, 41, 43, 45, 47, 51, 59, 101, 103 and 123 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:10, 32, 36, 38, 40, 42, 44, 46, 48, 52, 60, 102, 104 and 124.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:13, 33, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 119, 121 that codes for the polypeptide selected from the group consisting of SEQ ID NOs:14, 34, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 120 and 122.

›DETAILED DESCRIPTION OF THE INVENTION · 6 of 9

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:83, 85, 87, 89, 91, 93 and 95, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:84, 86, 88, 90, 92, 94 and 96.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:97, 109, 111, 113, 115 and 117, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:98, 110, 112, 114, 116 and 118.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:127, 129, 131 and 133, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:128, 130, 132 and 134.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:53, 135, 137, 139, 141 and 143, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:54, 136, 138, 140, 142 and 144.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165 and 167, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166 and 168.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:57, 105, 169, 171, 173, 175, 177, 179, 181 and 183, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:58, 106, 170, 172, 174, 176, 178, 180, 182 and 184.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:185, 187, 189, 191, 193, 195, 197, 199, 201 and 203, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:186, 188, 190, 192, 194, 196, 198, 200, 202 and 204.

Also preferably, the first nucleotide sequence comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:29, 55 and 107, that codes for the polypeptide selected from the group consisting of SEQ ID NOs:30, 56 and 108.

Nucleic acid fragments encoding at least a substantial portion of several metabolism proteins have been isolated and identified by comparison of random plant cDNA sequences to public databases containing nucleotide and protein sequences using the BLAST algorithms well known to those skilled in the art. The nucleic acid fragments of the instant invention may be used to isolate cDNAs and genes encoding homologous proteins from the same or other plant species. Isolation of homologous genes using sequence-dependent protocols is well known in the art. Examples of sequence-dependent protocols include, but are not limited to, methods of nucleic acid hybridization, and methods of DNA and RNA amplification as exemplified by various uses of nucleic acid amplification technologies (e.g., polymerase chain reaction, ligase chain reaction).

For example, genes encoding other dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter proteins, either as cDNAs or genomic DNAs, could be isolated directly by using all or a substantial portion of the instant nucleic acid fragments as DNA hybridization probes to screen libraries from any desired plant employing methodology well known to those skilled in the art. Specific oligonucleotide probes based upon the instant nucleic acid sequences can be designed and synthesized by methods known in the art (Maniatis). Moreover, an entire sequence(s) can be used directly to synthesize DNA probes by methods known to the skilled artisan such as random primer DNA labeling, nick translation, end-labeling techniques, or RNA probes using available in vitro transcription systems. In addition, specific primers can be designed and used to amplify a part or all of the instant sequences. The resulting amplification products can be labeled directly during amplification reactions or labeled after amplification reactions, and used as probes to isolate full length cDNA or genomic fragments under conditions of appropriate stringency.

In addition, two short segments of the instant nucleic acid fragments may be used in polymerase chain reaction protocols to amplify longer nucleic acid fragments encoding homologous genes from DNA or RNA. The polymerase chain reaction may also be performed on a library of cloned nucleic acid fragments wherein the sequence of one primer is derived from the instant nucleic acid fragments, and the sequence of the other primer takes advantage of the presence of the polyadenylic acid tracts to the 3′ end of the mRNA precursor encoding plant genes. Alternatively, the second primer sequence may be based upon sequences derived from the cloning vector. For example, the skilled artisan can follow the RACE protocol (Frohman et al. (1988) Proc. Natl. Acad. Sci. USA 85:8998-9002) to generate cDNAs by using PCR to amplify copies of the region between a single point in the transcript and the 3′ or 5′ end. Primers oriented in the 3′ and 5′ directions can be designed from the instant sequences. Using commercially available 3′ RACE or 5′ RACE systems (BRL), specific 3′ or 5′ cDNA fragments can be isolated (Ohara et al. (1989) Proc. Natl. Acad. Sci. USA 86:5673-5677; Loh et al. (1989) Science 243:217-220). Products generated by the 3′ and 5′ RACE procedures can be combined to generate full-length cDNAs (Frohman and Martin (1989) Techniques 1:165). Consequently, a polynucleotide comprising a nucleotide sequence of at least one of 60 (preferably one of at least 40, most preferably one of at least 30) contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183 185, 187, 189, 191, 193, 195, 197, 199, 201 and 203 and the complement of such nucleotide sequences may be used in such methods to obtain a nucleic acid fragment encoding a substantial portion of an amino acid sequence of a polypeptide.

›DETAILED DESCRIPTION OF THE INVENTION · 7 of 9

The present invention relates to a method of obtaining a nucleic acid fragment encoding a substantial portion of a dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide, preferably a substantial portion of a plant dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide, comprising the steps of: synthesizing an oligonucleotide primer comprising a nucleotide sequence of at least one of 60 (preferably at least one of 40, most preferably at least one of 30) contiguous nucleotides derived from a nucleotide sequence selected from the group consisting of SEQ ID NOs:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, 133, 135, 137, 139, 141, 143, 145, 147, 149, 151, 153, 155, 157, 159, 161, 163, 165, 167, 169, 171, 173, 175, 177, 179, 181, 183 185, 187, 189, 191, 193, 195, 197, 199, 201 and 203, and the complement of such nucleotide sequences; and amplifying a nucleic acid fragment (preferably a cDNA inserted in a cloning vector) using the oligonucleotide primer. The amplified nucleic acid fragment preferably will encode a portion of a dioxygenase, Ent-kaurene synthase A, Ent-Kaurene synthase B, GA-20 or Gibberellin 3-beta Hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase, GCN20-like ABC transporter, PDR5-like ABC transporter, P-glycoprotein, ABC transporter or MRP4 ABC transporter polypeptide.

Availability of the instant nucleotide and deduced amino acid sequences facilitates immunological screening of cDNA expression libraries. Synthetic peptides representing substantial portions of the instant amino acid sequences may be synthesized. These peptides can be used to immunize animals to produce polyclonal or monoclonal antibodies with specificity for peptides or proteins comprising the amino acid sequences. These antibodies can be then be used to screen cDNA expression libraries to isolate full-length cDNA clones of interest (Lerner (1984) Adv. Immunol. 36:1-34; Maniatis).

In another embodiment, this invention concerns viruses and host cells comprising either the chimeric genes of the invention as described herein or an isolated polynucleotide of the invention as described herein. Examples of host cells which can be used to practice the invention include, but are not limited to, yeast, bacteria, and plants.

As was noted above, the nucleic acid fragments of the instant invention may be used to create transgenic plants in which the disclosed polypeptides are present at higher or lower levels than normal or in cell types or developmental stages in which they are not normally found. This would have the effect of altering the level of activity of those proteins and subsequently modifying specific metabolic steps in those cells.

Overexpression of the proteins of the instant invention may be accomplished by first constructing a chimeric gene in which the coding region is operably linked to a promoter capable of directing expression of a gene in the desired tissues at the desired stage of development. The chimeric gene may comprise promoter sequences and translation leader sequences derived from the same genes. 3′ Non-coding sequences encoding transcription termination signals may also be provided. The instant chimeric gene may also comprise one or more introns in order to facilitate gene expression.

Plasmid vectors comprising the instant isolated polynucleotide (or chimeric gene) may be constructed. The choice of plasmid vector is dependent upon the method that will be used to transform host plants. The skilled artisan is well aware of the genetic elements that must be present on the plasmid vector in order to successfully transform, select and propagate host cells containing the chimeric gene. The skilled artisan will also recognize that different independent transformation events will result in different levels and patterns of expression (Jones et al. (1985) EMBO J. 4:2411-2418; De Almeida et al. (1989) Mol. Gen. Genetics 218:78-86), and thus that multiple events must be screened in order to obtain lines displaying the desired expression level and pattern. Such screening may be accomplished by Southern analysis of DNA, Northern analysis of mRNA expression, Western analysis of protein expression, or phenotypic analysis.

For some applications it may be useful to direct the instant polypeptides to different cellular compartments, or to facilitate their secretion from the cell. It is thus envisioned that the chimeric gene described above may be further supplemented by directing the coding sequence to encode the instant polypeptides with appropriate intracellular targeting sequences such as transit sequences (Keegstra (1989) Cell 56:247-253), signal sequences or sequences encoding endoplasmic reticulum localization (Chrispeels (1991) Ann. Rev. Plant Phys. Plant Mol. Biol. 42:21-53), or nuclear localization signals (Raikhel (1992) Plant Phys. 100:1627-1632) with or without removing targeting sequences that are already present. While the references cited give examples of each of these, the list is not exhaustive and more targeting signals of use may be discovered in the future.

It may also be desirable to reduce or eliminate expression of genes encoding the instant polypeptides in plants for some applications. In order to accomplish this, a chimeric gene designed for co-suppression of the instant polypeptide can be constructed by linking a gene or gene fragment encoding that polypeptide to plant promoter sequences. Alternatively, a chimeric gene designed to express anti sense RNA for all or part of the instant nucleic acid fragment can be constructed by linking the gene or gene fragment in reverse orientation to plant promoter sequences. Either the co-suppression or antisense chimeric genes could be introduced into plants via transformation wherein expression of the corresponding endogenous genes are reduced or eliminated.

›DETAILED DESCRIPTION OF THE INVENTION · 8 of 9

Molecular genetic solutions to the generation of plants with altered gene expression have a decided advantage over more traditional plant breeding approaches. Changes in plant phenotypes can be produced by specifically inhibiting expression of one or more genes by antisense inhibition or cosuppression (U.S. Pat. Nos. 5,190,931, 5,107,065 and 5,283,323). An antisense or cosuppression construct would act as a dominant negative regulator of gene activity. While conventional mutations can yield negative regulation of gene activity these effects are most likely recessive. The dominant negative regulation available with a transgenic approach may be advantageous from a breeding perspective. In addition, the ability to restrict the expression of a specific phenotype to the reproductive tissues of the plant by the use of tissue specific promoters may confer agronomic advantages relative to conventional mutations which may have an effect in all tissues in which a mutant gene is ordinarily expressed.

The person skilled in the art will know that special considerations are associated with the use of antisense or cosuppression technologies in order to reduce expression of particular genes. For example, the proper level of expression of sense or antisense genes may require the use of different chimeric genes utilizing different regulatory elements known to the skilled artisan. Once transgenic plants are obtained by one of the methods described above, it will be necessary to screen individual transgenics for those that most effectively display the desired phenotype. Accordingly, the skilled artisan will develop methods for screening large numbers of transformants. The nature of these screens will generally be chosen bn practical grounds. For example, one can screen by looking for changes in gene expression by using antibodies specific for the protein encoded by the gene being suppressed, or one could establish assays that specifically measure enzyme activity. A preferred method will be one which allows large numbers of samples to be processed rapidly, since it will be expected that a large number of transformants will be negative for the desired phenotype.

In another embodiment, the present invention concerns a polypeptide of at least 351 amino acids that has at least 80% identity-based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:2, 4, 6, 8, 50, 82 and 100.

In another embodiment, the present invention concerns a polypeptide of at least 72 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:12, 16, 18, 20, 22, 24, 26 and 28.

In another embodiment, the present invention concerns a polypeptide of at least 110 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:10, 32, 36, 38, 40, 42, 44, 46, 48, 52, 60, 102, 104 and 124.

In another embodiment, the present invention concerns a polypeptide of at least 189 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:14, 34, 62, 64, 68, 70, 72, 74, 76, 78, 80, 120 and 122.

In another embodiment, the present invention concerns a polypeptide of at least 67 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:84, 86, 88, 90, 92, 94 and 96.

In another embodiment, the present invention concerns a polypeptide of at least 87 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:98, 110, 112, 114, 116 and 118.

In another embodiment, the present invention concerns a polypeptide of at least 155 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:128, 130, 132 and 134.

In another embodiment, the present invention concerns a polypeptide of at least 161 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:54, 136, 138, 140, 142 and 144.

In another embodiment, the present invention concerns a polypeptide of at least 66 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166 and 168.

In another embodiment, the present invention concerns a polypeptide of at least 141 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID NOs:58, 106, 170, 172, 174, 176, 178, 180, 182 and 184.

In another embodiment, the present invention concerns a polypeptide of at least 81 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID:186, 188, 190, 192, 194, 196, 198, 200, 202 and 204.

In another embodiment, the present invention concerns a polypeptide of at least 48 amino acids that has at least 80% identity based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of SEQ ID:30, 56 and 108.

The instant polypeptides (or substantial portions thereof) may be produced in heterologous host cells, particularly in the cells of microbial hosts, and can be used to prepare antibodies to these proteins by methods well known to those skilled in the art. The antibodies are useful for detecting the polypeptides of the instant invention in situ in cells or in vitro in cell extracts. Preferred heterologous host cells for production of the instant polypeptides are microbial hosts. Microbial expression systems and expression vectors containing regulatory sequences that direct high level expression of foreign proteins are well known to those skilled in the art. Any of these could be used to construct a chimeric gene for production of the instant polypeptides. This chimeric gene could then be introduced into appropriate microorganisms via transformation to provide high level expression of the encoded metabolism protein. An example of a vector for high level expression of the instant polypeptides in a bacterial host is provided (Example 6).

›DETAILED DESCRIPTION OF THE INVENTION · 9 of 9

All or a substantial portion of the polynucleotides of the instant invention may also be used as probes for genetically and physically mapping the genes that they are a part of, and used as markers for traits linked to those genes. Such information may be useful in plant breeding in order to develop lines with desired phenotypes. For example, the instant nucleic acid fragments may be used as restriction fragment length polymorphism (RFLP) markers. Southern blots (Maniatis) of restriction-digested plant genomic DNA may be probed with the nucleic acid fragments of the instant invention. The resulting banding patterns may then be subjected to genetic analyses using computer programs such as MapMaker (Lander et al. (1987) Genomics 1:174-181) in order to construct a genetic map. In addition, the nucleic acid fragments of the instant invention may be used to probe Southern blots containing restriction endonuclease-treated genomic DNAs of a set of individuals representing parent and progeny of a defined genetic cross. Segregation of the DNA polymorphisms is noted and used to calculate the position of the instant nucleic acid sequence in the genetic map previously obtained using this population (Botstein et al. (1980) Am. J Hum. Genet. 32:314-331).

The production and use of plant gene-derived probes for use in genetic mapping is described in Bernatzky and Tanksley (1986) Plant Mol. Biol. Reporter 4:37-41. Numerous publications describe genetic mapping of specific cDNA clones using the methodology outlined above or variations thereof. For example, F2 intercross populations, backcross populations, randomly mated populations, near isogenic lines, and other sets of individuals may be used for mapping. Such methodologies are well known to those skilled in the art.

Nucleic acid probes derived from the instant nucleic acid sequences may also be used for physical mapping (i.e., placement of sequences on physical maps; see Hoheisel et al. In: Nonmammalian Genomic Analysis: A Practical Guide, Academic press 1996, pp. 319-346, and references cited therein).

In another embodiment, nucleic acid probes derived from the instant nucleic acid sequences may be used in direct fluorescence in situ hybridization (FISH) mapping (Trask (1991) Trends Genet. 7:149-154). Although current methods of FISH mapping favor use of large clones (several to several hundred KB; see Laan et al. (1995) Genome Res. 5:13-20), improvements in sensitivity may allow performance of FISH mapping using shorter probes.

A variety of nucleic acid amplification-based methods of genetic and physical mapping may be carried out using the instant nucleic acid sequences. Examples include allele-specific amplification (Kazazian (1989) J. Lab. Clin. Med. 11:95-96), polymorphism of PCR-amplified fragments (CAPS; Sheffield et al. (1993) Genomics 16:325-332), allele-specific ligation (Landegren et al. (1988) Science 241:1077-1080), nucleotide extension reactions (Sokolov (1990) Nucleic Acid Res. 18:3671), Radiation Hybrid Mapping (Walter et al. (1997) Nat. Genet. 7:22-28) and Happy Mapping (Dear and Cook (1989) Nucleic Acid Res. 17:6795-6807). For these methods, the sequence of a nucleic acid fragment is used to design and produce primer pairs for use in the amplification reaction or in primer extension reactions. The design of such primers is well known to those skilled in the art. In methods employing PCR-based genetic mapping, it may be necessary to identify DNA sequence differences between the parents of the mapping cross in the region corresponding to the instant nucleic acid sequence. This, however, is generally not necessary for mapping methods.

Loss of function mutant phenotypes may be identified for the instant cDNA clones either by targeted gene disruption protocols or by identifying specific mutants for these genes contained in a maize population carrying mutations in all possible genes (Ballinger and Benzer (1989) Proc. Natl. Acad. Sci USA 86:9402-9406; Koes et al. (1995) Proc. Natl. Acad. Sci USA 92:8149-8153; Bensen et al. (1995) Plant Cell 7:75-84). The latter approach may be accomplished in two ways. First, short segments of the instant nucleic acid fragments may be used in polymerase chain reaction protocols in conjunction with a mutation tag sequence primer on DNAs prepared from a population of plants in which Mutator transposons or some other mutation-causing DNA element has been introduced (see Bensen, supra). The amplification of a specific DNA fragment with these primers indicates the insertion of the mutation tag element in or near the plant gene encoding the instant polypeptides. Alternatively, the instant nucleic acid fragment may be used as a hybridization probe against PCR amplification products generated from the mutation population using the mutation tag sequence primer in conjunction with an arbitrary genomic site primer, such as that for a restriction enzyme site-anchored synthetic adaptor. With either method, a plant containing a mutation in the endogenous gene encoding the instant polypeptides can be identified and obtained. This mutant plant can then be used to determine or confirm the natural function of the instant polypeptides disclosed herein.

›EXAMPLES

The present invention is further defined in the following Examples, in which parts and percentages are by weight and degrees are Celsius, unless otherwise stated. It should be understood that these Examples, while indicating preferred embodiments of the invention, are given by way of illustration only. From the above discussion and these Examples, one skilled in the art can ascertain the essential characteristics of this invention, and without departing from the spirit and scope thereof, can make various changes and modifications of the invention to adapt it to various usages and conditions. Thus, various modifications of the invention in addition to those shown and described herein will be apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims.

The disclosure of each reference set forth herein is incorporated herein by reference in its entirety.

›Examples7
›Example 1

Composition of cDNA Libraries, Isolation and Sequencing of cDNA Clones

cDNA libraries representing mRNAs from various corn, rice, soybean and wheat tissues were prepared. The characteristics of the libraries are described below.

cDNA libraries may be prepared by any one of many methods available. For example, the cDNAs may be introduced into plasmid vectors by first preparing the cDNA libraries in Uni-ZAP™ XR vectors according to the manufacturer's protocol (Stratagene Cloning Systems, La Jolla, Calif.). The Uni-ZAP™ XR libraries are converted into plasmid libraries according to the protocol provided by Stratagene. Upon conversion, cDNA inserts will be contained in the plasmid vector pBluescript. In addition, the cDNAs may be introduced directly into precut Bluescript II SK(+) vectors (Stratagene) using T4 DNA ligase (New England Biolabs), followed by transfection into DH10B cells according to the manufacturer's protocol (GIBCO BRL Products). Once the cDNA inserts are in plasmid vectors, plasmid DNAs are prepared from randomly picked bacterial colonies containing recombinant pBluescript plasmids, or the insert cDNA sequences are amplified via polymerase chain reaction using primers specific for vector sequences flanking the inserted cDNA sequences. Amplified insert DNAs or plasmid DNAs are sequenced in dye-primer sequencing reactions to generate partial cDNA sequences (expressed sequence tags or “ESTs”; see Adams et al., (1991) Science 252:1651-1656). The resulting ESTs are analyzed using a Perkin Elmer Model 377 fluorescent sequencer.

›Example 2

Identification of cDNA Clones

cDNA clones encoding transcription factors were identified by conducting BLAST (Basic Local Alignment Search Tool; Altschul et al. (1993) J. Mol. Biol. 215:403-410) searches for similarity to sequences contained in the BLAST “nr” database (comprising all non-redundant GenBank CDS translations, sequences derived from the 3-dimensional structure Brookhaven Protein Data Bank, the last major release of the SWISS-PROT protein sequence database, EMBL, and DDBJ databases). The cDNA sequences obtained in Example 1 were analyzed for similarity to all publicly available DNA sequences contained in the “nr” database using the BLASTN algorithm provided by the National Center for Biotechnology Information (NCBI). The DNA sequences were translated in all reading frames and compared for similarity to all publicly available protein sequences contained in the “nr” database using the BLASTX algorithm (Gish and States (1993) Nat. Genet. 3:266-272) provided by the NCBI. For convenience, the P-value (probability) of observing a match of a cDNA sequence to a sequence contained in the searched databases merely by chance as calculated by BLAST are reported herein as “pLog” values, which represent the negative of the logarithm of the reported P-value. Accordingly, the greater the pLog value, the greater the likelihood that the cDNA sequence and the BLAST “hit” represent homologous proteins.

›Example 3 · 1 of 2

Characterization of cDNA Clones Encoding Plant Metabolism Proteins

The BLASTX search using the EST sequences from clones listed in Table 3 revealed similarity of the polypeptides encoded by the cDNAs to the following proteins.

Dioxygenase from Marah macrocarpus (NCBI General Identifier No. gi 1666096).

Ent-Kaurene synthase A from Cucurbita maxima (NCBI General Identifer No. gi 4151195), Zea mays (NCBI General Identifer No. gi 7489781), Stevia rebaudiana (NCBI General Identifer No. gi 2642661) and Pisum sativum (NCBI General Identifer No. gi 2160544).

Ent-Kaurene synthase B from Cucurbita maxima (NCBI General Identifer No. gi 74847663), Cucurbita maxima (NCBI General Identifer No. gi 1431870) and Arabidopsis thaliana (NCBI General Identifer No. gi 3056725).

GA-20 oxidase from Triticum aestivum (NCBI Identification No. gi 2222798) and Pisum sativum (NCBI Identification No. gi 1381673).

Gibberellin 3-beta hydroxylase from Lycopersicon esculentum (NCBI Identifier No. gi 3834350), Lactuca sativa (NCBI Identifier No. gi 4164145) and Pisum sativum (NCBI Identifier No. gi 2316102).

GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase from Arabidopsis thaliana (NCBI General Identifer No. gi 2462925).

Riboflavin specific deaminase from Arabidopsis thaliana (NCBI General Identifer No. gi 5262786) and Arabidopsis thaliana (NCBI General Identifer No. gi 5541706).

Ethylene response factor from Phalaenopsis sp. (NCBI General Identifier No. gi 4650821), Oryza sativa (NCBI General Identifier No. gi 2281705), Vigna radiata (NCBI General Identifier No. gi 4138853), Lycopersicon esculentum (NCBI General Identifier No. gi 4877653) and Nicotiana tabacum (NCBI General dentifier No. gi 5733831).

Acyl-CoA thioesterase from Arabidopsis thaliana (NCBI General Identifier No. gi 3047124), Homo sapiens (NCBI General Identifier No. gi 4885565), Caenorhabditis elegans (NCBI General Identifier No. gi 1213545).

ABC transporter GCN20-like from Arabidopsis thaliana (NCBI General Identifier No. gi 6633814).

ABC transporter PDR5-like from Arabidopsis thaliana (NCBI General Identifier No. gi 4581139), Arabidopsis thaliana (NCBI General Identifier No. gi 2062169), Arabidopsis thaliana (NCBI General Identifier No. gi 8072390) and Spirodela polyrrhiza (NCBI General Identifier No. gi 4581139).

P-glycoproteins (1, 3, ATPGP and HVMDR2) from Arabidopsis thaliana (NCBI General Identifier No. gi 7268557), Arabidopsis thaliana (NCBI General Identifier No. gi 4558552), Arabidopsis thaliana (NCBI General Identifier No. gi 3522943), Mus musculus (NCBI General Identifier No. gi 8571454), Arabidopsis thaliana (NCBI General Identifier No. gi 2739309), Oryza sativa (NCBI General Identifier No. gi 8468012), Arabidopsis thaliana (NCBI General Identifier No. gi 6573748), Arabidopsis thaliana (NCBI General Identifier No. gi 7486813) and Hordeum vulgare (NCBI General Identifier No. gi 3047124), Arabadopsis thaliana (NCBI General Indentifier No. gi 7442648) and Hordeum vulgare (NCBI General Identifier No. gi 7442649).

ABC transporter proteins from Arabidopsis thaliana (NCBI General Identifier No. gi 4115931), Arabidopsis thaliana (NCBI General Identifier No. gi 7487703) and Arabidopsis thaliana (NCBI General Identifier No. gi 4585979).

MRP4 ABC transproter proteins from Arabidopsis thaliana (NCBI General Identifier No. gi 7485868), Arabidopsis thaliana (NCBI General Identifier No. gi 2980641) and Arabidopsis thaliana (NCBI General Identifier No. gi 7362750), Arabidopsis thaliana (NCBI General Identifier No. gi 2316016), Arabidopsis thaliana (NCBI General Identifier No. gi 2959767) and Arabidopsis thaliana (NCBI General Identifier No. gi 7076769).

Shown in Table 3 are the BLAST results for individual ESTs (“EST”), the sequences of the entire cDNA inserts comprising the indicated cDNA clones (“FIS”), the sequences of contigs assembled from two or more ESTs (“Contig”), sequences of contigs assembled from an FIS and one or more ESTs (“Contig*”), or sequences encoding an entire protein derived from an FIS, a contig, or an FIS and PCR (“CGS”):

The sequence of the entire cDNA insert in the clones listed in Table 3 was determined. Further sequencing and searching of the DuPont proprietary database allowed the identification of other corn, rice, soybean and/or wheat clones encoding dioxygenase, Ent-kaurene synthase A, Ent-kaurene synthase B, GA-20 oxidase, gibberellin 3-beta hydroxylase, GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase, riboflavin specific deaminase, ethylene response factor, acyl-CoA thioesterase.

The BLASTX search using the EST sequences from clones listed in Table 4 revealed similarity of the polypeptides encoded by the cDNAs to the following proteins.

Dioxygenase from Marah macrocarpus (NCBI General Identifier No. gi 1666096) and Phaseolus coccineus (NCBI General Identifier No. gi 4678586).

Ent-kaurene synthase A from Cucurbita maxima (NCBI General Identifer No. gi 4151195), Zea mays (NCBI General Identifer No. gi 7489781), and Pisum sativum (NCBI General Identifer No. gi 2160544).

Ent-kaurene synthase B from Cucurbita maxima (NCBI General Identifer No. gi 7484763), and Arabidopsis thaliana (NCBI General Identifer No. gi 3056725).

GA-20 oxidase from Triticum aestivum (NCBI General Identifer No. gi 7433220), Triticum aestivum (NCBI General Identifer No. gi 2222798) and Pisum sativum (NCBI General Identifer No. gi 1381673).

Gibberellin 3-beta hydroxylase from Pisum sativum (NCBI General Identifer No. gi 5813796), Lactuca sativa (NCBI General Identifer No. gi 4164145), Arabdopsis thaliana (NCBI General Identifer No. gi 3924597) and Lycoperscon esculentum (NCBI General Identifer No. gi 3834350).

GTP cyclohydrolase II/3,4-dihydroxy-2-butanone-4-phosphate synthase from Arabidopsis thaliana (NCBI General Identifer No. gi 2462925) and Arabidopsis thaliana (NCBI General Identifer No. gi 1346113).

Riboflavin specific deaminase from Arabidopsis thaliana (NCBI General Identifer No. gi 5262786) and Arabidopsis thaliana (NCBI General Identifer No. gi 5541706).

›Example 3 · 2 of 2

Ethylene response factor from Phalaenopsis sp. (NCBI General Identifier No. gi 4650821), Phalaenopsis sp. (NCBI General Identifier No. gi 4154359), Cucumis sativus (NCBI General Identifier No. gi 6136818), Oryza sativa (NCBI General dentifier No. gi 7489538), Oryza sativa (NCBI General dentifier No. gi 2281705), Vigna radiata (NCBI General dentifier No. gi 4138853), and Lycopersicon esculentum (NCBI General dentifier No. gi 4877653).

Acyl-CoA thioesterase from Arabidopsis thaliana (NCBI General Identifier No. gi 3047124), Homo sapiens (NCBI General Identifier No. gi 4885565), Caenorhabditis elegans (NCBI General Identifier No. gi 1213545).

Shown in Table 4 are the BLAST results for individual ESTs (“EST”), the sequences of the entire cDNA inserts comprising the indicated cDNA clones (“FIS”), sequences of contigs assembled from two or more ESTs (“Contig”), sequences of contigs assembled from an FIS and one or more ESTs (“Contig*”), or sequences encoding the entire protein derived from an FIS, a contig, or an FIS and PCR (“CGS”):

The data in Table 5 represents a calculation of the percent identity of the amino acid sequences set forth in SEQ ID NOs:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202 and 204 and the homologous sequence from the above identified species.

Sequence alignments and percent identity calculations were performed using the Megalign program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis. ). Multiple alignment of the sequences was performed using the Clustal method of alignment (Higgins and Sharp (1989) CABIOS. 5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments using the Clustal method were KTUPLE 1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5. Sequence alignments, BLAST scores and probabilities indicate that the nucleic acid fragments comprising the instant cDNA clones encode a substantial portion of various plant metabolism proteins.

›Example 4

Expression of Chimeric Genes in Monocot Cells

A chimeric gene comprising a cDNA encoding the instant polypeptides in sense orientation with respect to the maize 27 kD zein promoter that is located 5′ to the cDNA fragment, and the 10 kD zein 3′ end that is located 3′ to the cDNA fragment, can be constructed. The cDNA fragment of this gene may be generated by polymerase chain reaction (PCR) of the cDNA clone using appropriate oligonucleotide primers. Cloning sites (NcoI or SmaI) can be incorporated into the oligonucleotides to provide proper orientation of the DNA fragment when inserted into the digested vector pML 103 as described below. Amplification is then performed in a standard PCR. The amplified DNA is then digested with restriction enzymes NcoI and SmaI and fractionated on an agarose gel. The appropriate band can be isolated from the gel and combined with a 4.9 kb NcoI-SmaI fragment of the plasmid pML 103. Plasmid pML 103 has been deposited under the terms of the Budapest Treaty at ATCC (American Type Culture Collection, 10801 University Blvd., Manassas, Va. 20110-2209), and bears accession number ATCC 97366. The DNA segment from pML 103 contains a 1.05 kb SalI-NcoI promoter fragment of the maize 27 kD zein gene and a 0.96 kb SmaI-SalI fragment from the 3′ end of the maize 10 kD zein gene in the vector pGem9Zf(+) (Promega). Vector and insert DNA can be ligated at 15° C. overnight, essentially as described (Maniatis). The ligated DNA may then be used to transform E. coli XL1-Blue (Epicurian Coli XL-1 Blue™; Stratagene). Bacterial transformants can be screened by restriction enzyme digestion of plasmid DNA and limited nucleotide sequence analysis using the dideoxy chain termination method (Sequenase™ DNA Sequencing Kit; U.S. Biochemical). The resulting plasmid construct would comprise a chimeric gene encoding, in the 5′ to 3′ direction, the maize 27 kD zein promoter, a cDNA fragment encoding the instant polypeptides, and the 10 kD zein 3′ region.

The chimeric gene described above can then be introduced into corn cells by the following procedure. Immature corn embryos can be dissected from developing caryopses derived from crosses of the inbred corn lines H99 and LH132. The embryos are isolated 10 to 11 days after pollination when they are 1.0 to 1.5 mm long. The embryos are then placed with the axis-side facing down and in contact with agarose-solidified N6 medium (Chu et al. (1975) Sci. Sin. Peking 18:659-668). The embryos are kept in the dark at 27° C. Friable embryogenic callus consisting of undifferentiated masses of cells with somatic proembryoids and embryoids borne on suspensor structures proliferates from the scutellum of these immature embryos. The embryogenic callus isolated from the primary explant can be cultured on N6 medium and sub-cultured on this medium every 2 to 3 weeks.

The plasmid, p35S/Ac (obtained from Dr. Peter Eckes, Hoechst Ag, Frankfurt, Germany) may be used in transformation experiments in order to provide for a selectable marker. This plasmid contains the Pat gene (see European Patent Publication 0 242 236) which encodes phosphinothricin acetyl transferase (PAT). The enzyme PAT confers resistance to herbicidal glutamine synthetase inhibitors such as phosphinothricin. The pat gene in p35S/Ac is under the control of the 35S promoter from Cauliflower Mosaic Virus (Odell et al. (1985) Nature 313:810-812) and the 3′ region of the nopaline synthase gene from the T-DNA of the Ti plasmid of Agrobacterium tumefaciens.

The particle bombardment method (Klein et al. (1987) Nature 327:70-73) may be used .to transfer genes to the callus culture cells. According to this method, gold particles (1 μm in diameter) are coated with DNA using the following technique. Ten μg of plasmid DNAs are added to 50 μL of a suspension of gold particles (60 mg per mL). Calcium chloride (50 μL of a 2.5 M solution) and spermidine free base (20 μL of a 1.0 M solution) are added to the particles. The suspension is vortexed during the addition of these solutions. After 10 minutes, the tubes are briefly centrifuged (5 sec at 15,000 rpm) and the supernatant removed. The particles are resuspended in 200 μL of absolute ethanol, centrifuged again and the supernatant removed. The ethanol rinse is performed again and the particles resuspended in a final volume of 30 μL of ethanol. An aliquot (5 μL) of the DNA-coated gold particles can be placed in the center of a Kapton™ flying disc (Bio-Rad Labs). The particles are then accelerated into the corn tissue with a Biolistic™ PDS-1000/He (Bio-Rad Instruments, Hercules Calif.), using a helium pressure of 1000 psi, a gap distance of 0.5 cm and a flying distance of 1.0 cm.

For bombardment, the embryogenic tissue is placed on filter paper over agarose-solidified N6 medium. The tissue is arranged as a thin lawn and covered a circular area of about 5 cm in diameter. The petri dish containing the tissue can be placed in the chamber of the PDS-1000/He approximately 8 cm from the stopping screen. The air in the chamber is then evacuated to a vacuum of 28 inches of mercury (Hg). The macrocarrier is accelerated with a helium shock wave using a rupture membrane that bursts when the He pressure in the shock tube reaches 1000 psi.

Seven days after bombardment the tissue can be transferred to N6 medium that contains gluphosinate (2 mg per liter) and lacks casein or proline. The tissue continues to grow slowly on this medium. After an additional 2 weeks the tissue can be transferred to fresh N6 medium containing gluphosinate. After 6 weeks, areas of about 1 cm in diameter of actively growing callus can be identified on some of the plates containing the glufosinate-supplemented medium. These calli may continue to grow when sub-cultured on the selective medium.

Plants can be regenerated from the transgenic callus by first transferring clusters of tissue to N6 medium supplemented with 0.2 mg per liter of 2,4-D. After two weeks the tissue can be transferred to regeneration medium (Fromm et al. (1990) Bio/Technology 8:833-839).

›Example 5

Expression of Chimeric Genes in Dicot Cells

A seed-specific construct composed of the promoter and transcription terminator from the gene encoding the β subunit of the seed storage protein phaseolin from the bean Phaseolus vulgaris (Doyle et al. (1986) J. Biol. Chem. 261:9228-9238) can be used for expression of the instant polypeptides in transformed soybean. The phaseolin construct includes about 500 nucleotides upstream (5′) from the translation initiation codon and about 1650 nucleotides downstream (3′) from the translation stop codon of phaseolin. Between the 5′ and 3′ regions are the unique restriction endonuclease sites Nco I (which includes the ATG translation initiation codon), Sma I, Kpn I and Xba I. The entire construct is flanked by Hind III sites.

The cDNA fragment of this gene may be generated by polymerase chain reaction (PCR) of the cDNA clone using appropriate oligonucleotide primers. Cloning sites can be incorporated into the oligonucleotides to provide proper orientation of the DNA fragment when inserted into the expression vector. Amplification is then performed as described above, and the isolated fragment is inserted into a pUC18 vector carrying the seed construct.

Soybean embryos may then be transformed with the expression vector comprising sequences encoding the instant polypeptides. To induce somatic embryos, cotyledons, 3-5 mm in length dissected from surface sterilized, immature seeds of the soybean cultivar A2872, can be cultured in the light or dark at 26° C. on an appropriate agar medium for 6-10 weeks. Somatic embryos which produce secondary embryos are then excised and placed into a suitable liquid medium. After repeated selection for clusters of somatic embryos which multiplied as early, globular staged embryos, the suspensions are maintained as described below.

Soybean embryogenic suspension cultures can be maintained in 35 mL liquid media on a rotary shaker, 150 rpm, at 26° C. with florescent lights on a 16:8 hour day/night schedule. Cultures are subcultured every two weeks by inoculating approximately 35 mg of tissue into 35 mL of liquid medium.

Soybean embryogenic suspension cultures may then be transformed by the method of particle gun bombardment (Klein et al. (1987) Nature (London) 327:70-73, U.S. Pat. No. 4,945,050). A DuPont Biolistic™ PDS1000/HE instrument (helium retrofit) can be used for these transformations.

A selectable marker gene which can be used to facilitate soybean transformation is a chimeric gene composed of the 35S promoter from Cauliflower Mosaic Virus (Odell et al. (1985) Nature 313:810-812), the hygromycin phosphotransferase gene from plasmid pJR225 (from E. coli ; Gritz et al.(1983) Gene 25:179-188) and the 3′ region of the nopaline synthase gene from the T-DNA of the Ti plasmid of Agrobacterium tumefaciens . The seed construct comprising the phaseolin 5′ region, the fragment encoding the instant polypeptides and the phaseolin 3′ region can be isolated as a restriction fragment. This fragment can then be inserted into a unique restriction site of the vector carrying the marker gene.

To 50 μL of a 60 mg/mL 1 μm gold particle suspension is added (in order): 5 μL DNA (1 μg/μL), 20 μL spermidine (0.1 M), and 50 μL CaCl 2 (2.5 M). The particle preparation is then agitated for three minutes, spun in a microfuge for 10 seconds and the supernatant removed. The DNA-coated particles are then washed once in 400 μL 70% ethanol and resuspended in 40 μL of anhydrous ethanol. The DNA/particle suspension can be sonicated three times for one second each. Five μL of the DNA-coated gold particles are then loaded on each macro carrier disk.

Approximately 300-400 mg of a two-week-old suspension culture is placed in an empty 60×15 mm petri dish and the residual liquid removed from the tissue with a pipette. For each transformation experiment, approximately 5-10 plates of tissue are normally bombarded. Membrane rupture pressure is set at 1100 psi and the chamber is evacuated to a vacuum of 28 inches of mercury (Hg). The tissue is placed approximately 3.5 inches away from the retaining screen and bombarded three times. Following bombardment, the tissue can be divided in half and placed back into liquid and cultured as described above.

Five to seven days post bombardment, the liquid media may be exchanged with fresh media, and eleven to twelve days post bombardment with fresh media containing 50 mg/mL hygromycin. This selective media can be refreshed weekly. Seven to eight weeks post bombardment, green, transformed tissue may be observed growing from untransformed, necrotic embryogenic clusters. Isolated green tissue is removed and inoculated into individual flasks to generate new, clonally propagated, transformed embryogenic suspension cultures. Each new line may be treated as an independent transformation event. These suspensions can then be subcultured and maintained as clusters of immature embryos or regenerated into whole plants by maturation and germination of individual somatic embryos.

›Example 6

Expression of Chimeric Genes in Microbial Cells

The cDNAs encoding the instant polypeptides can be inserted into the T7 E. coli expression vector pBT430. This vector is a derivative of pET-3a (Rosenberg et al. (1987) Gene 56:125-135) which employs the bacteriophage T7 RNA polymerase/T7 promoter system. Plasmid pBT430 was constructed by first destroying the EcoR I and Hind III sites in pET-3a at their original positions. An oligonucleotide adaptor containing EcoR I and Hind III sites was inserted at the BamH I site of pET-3a. This created pET-3aM with additional unique cloning sites for insertion of genes into the expression vector. Then, the Nde I site at the position of translation initiation was converted to an Nco I site using oligonucleotide-directed mutagenesis. The DNA sequence of pET-3aM in this region, 5′-CATATGG, was converted to 5′-CCCATGG in pBT430.

Plasmid DNA containing a cDNA may be appropriately digested to release a nucleic acid fragment encoding the protein. This fragment may then be purified on a 1% low melting agarose gel. Buffer and agarose contain 10 μg/mL ethidium bromide for visualization of the DNA fragment. The fragment can then be purified from the agarose gel by digestion with GELase™ (Epicentre Technologies, Madison, Wis.) according to the manufacturer's instructions, ethanol precipitated, dried and resuspended in 20 μL of water. Appropriate oligonucleotide adapters may be ligated to the fragment using T4 DNA ligase (New England Biolabs (NEB), Beverly, Mass.). The fragment containing the ligated adapters can be purified from the excess adapters using low melting agarose as described above. The vector pBT430 is digested, dephosphorylated with alkaline phosphatase (NEB) and deproteinized with phenol/chloroform as described above. The prepared vector pBT430 and fragment can then be ligated at 16° C. for 15 hours followed by transformation into DH5 electrocompetent cells (GIBCO BRL). Transformants can be selected on agar plates containing LB media and 100 μ/mL ampicillin. Transformants containing the gene encoding the instant polypeptides are then screened for the correct orientation with respect to the T7 promoter by restriction enzyme analysis.

For high level expression, a plasmid clone with the cDNA insert in the correct orientation relative to the T7 promoter can be transformed into E. coli strain BL21(DE3) (Studier et al. (1986) J. Mol. Biol. 189:113-130). Cultures are grown in LB medium containing ampicillin (100 mg/L) at 25° C. At an optical density at 600 nm of approximately 1, IPTG (isopropylthio-β-galactoside, the inducer) can be added to a final concentration of 0.4 mM and incubation can be continued for 3 h at 25° C. Cells are then harvested by centrifugation and re-suspended in 50 μL of 50 mM Tris-HCl at pH 8.0 containing 0.1 mM DTT and 0.2 mM phenyl methylsulfonyl fluoride. A small amount of 1 mm glass beads can be added and the mixture sonicated 3 times for about 5 seconds each time with a microprobe sonicator. The mixture is centrifuged and the-protein concentration of the supernatant determined. One μg of protein from the soluble fraction of the culture can be separated by SDS-polyacrylamide gel electrophoresis. Gels can be observed for protein bands migrating at the expected molecular weight.

›SEQUENCE LISTING · 1 of 53

<160> NUMBER OF SEQ ID NOS: 204

<210> SEQ ID NO 1

<211> LENGTH: 1555

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 1

cacgcacacc gcctcgactc gactcccacc tcccctgttc tcaccgtgct tgccctgttt 60

ccacctctgc tgcccatccg cctcgcttgc ccgccgtaca gtacaggcca gacagccatg 120

gtggtgctcg ccaaaccgcc tgtcgtcgac cagatcccgc tcctgcggtc cccgggcccc 180

agggacagct tctcgggagt gccggtcgtc gacctgtcca gccacggcgc ggcgcgggcg 240

atcgtcgacg cctgcgagcg cttcgggttc ttcaaggtcg tcaaccacgg cgtggccgcg 300

gccaccatgg acagggccga gtccgaggcc gtcaggttct tcgcgcaggc gcaggcggac 360

aaggaccgcg cggggccggc gtacccgttc gggtacggca gcaagcggat cgggctcaat 420

ggcgacatgg ggtggctcga gtacctcctc ctcgccgtcg acgccgcgtc gctctccgac 480

gcctgccccg tgccctccag cgccgcgttc cggagcgcgc tgaacgagta cgtcgcggcc 540

gtgcggaagg tggcggcgcg tgtgctggag gcgatggcgg agggcctggg cattgcggac 600

gcggacgcgc tgagctccat ggtgagcggc gccgggagcg accaggtgtt ccgcgtgaac 660

cactacccgc cctgccccgc gctgcagggc ctgggctgca gcaccacggg cttcggcgag 720

cacaccgacc cgcagatcat ctccgtgctc cgctccaacg gcacctccgg cctgcagatc 780

gcgctccgcg acggcgcgca gtgggtctcc gtgccctccg accgcgacgc cttcttcgtt 840

aacgtcggcg actcgttgca ggtgctgacc aacgggaggt tcaggagcgt gaagcaccgg 900

gtggtgacca acagcctcaa gtccagagtt tccttcatct acttcgcggg gccgccgctg 960

gggcagcgga tcgcgccgct gccgcaggtg ctggcggagg gagaggagag cctgtacaag 1020

gagttcacgt ggggcgagta caagaaggcc gcgtacaaga cgaggctcgg cgacaacagg 1080

ctggcccagt ttgagaagcg tagcaacatc tagctaagct agcaaggcgc gcggcccggg 1140

catgcacgcc gcgtcttcac aggacaacaa taacaaccac catgacgagc gagttacttg 1200

caagaaatga tgacaaggaa gaagaagaag aagaaggtga agacgatgat gtgaacagcc 1260

agggagcgta gtgactactc cagtagctag atttttttgt gtggtgggtg cagggtttcc 1320

gcaaagttgc taatggcctg ttctggtgct tgcggtgggg ttagttgcct atttcggtat 1380

gctggagctg gacgggcgat gagccgccgc ctgtaattag ggttaaccat gatagcatgc 1440

agcgaagcga aggggacgat cctctctggc tccgtagctt cccacgtttg cttccttgtg 1500

ctttcccttt cctggtttat atatattttc gtataaaaaa aaaaaaaaaa aaaag 1555

<210> SEQ ID NO 2

<211> LENGTH: 370

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 2

His Ala His Arg Leu Asp Ser Thr Pro Thr Ser Pro Val Leu Thr Val

1 5 10 15

Leu Ala Leu Phe Pro Pro Leu Leu Pro Ile Arg Leu Ala Cys Pro Pro

20 25 30

Tyr Ser Thr Gly Gln Thr Ala Met Val Val Leu Ala Lys Pro Pro Val

35 40 45

Val Asp Gln Ile Pro Leu Leu Arg Ser Pro Gly Pro Arg Asp Ser Phe

50 55 60

Ser Gly Val Pro Val Val Asp Leu Ser Ser His Gly Ala Ala Arg Ala

65 70 75 80

Ile Val Asp Ala Cys Glu Arg Phe Gly Phe Phe Lys Val Val Asn His

85 90 95

Gly Val Ala Ala Ala Thr Met Asp Arg Ala Glu Ser Glu Ala Val Arg

100 105 110

Phe Phe Ala Gln Ala Gln Ala Asp Lys Asp Arg Ala Gly Pro Ala Tyr

115 120 125

Pro Phe Gly Tyr Gly Ser Lys Arg Ile Gly Leu Asn Gly Asp Met Gly

130 135 140

Trp Leu Glu Tyr Leu Leu Leu Ala Val Asp Ala Ala Ser Leu Ser Asp

145 150 155 160

Ala Cys Pro Val Pro Ser Ser Ala Ala Phe Arg Ser Ala Leu Asn Glu

165 170 175

Tyr Val Ala Ala Val Arg Lys Val Ala Ala Arg Val Leu Glu Ala Met

180 185 190

Ala Glu Gly Leu Gly Ile Ala Asp Ala Asp Ala Leu Ser Ser Met Val

195 200 205

Ser Gly Ala Gly Ser Asp Gln Val Phe Arg Val Asn His Tyr Pro Pro

210 215 220

Cys Pro Ala Leu Gln Gly Leu Gly Cys Ser Thr Thr Gly Phe Gly Glu

225 230 235 240

His Thr Asp Pro Gln Ile Ile Ser Val Leu Arg Ser Asn Gly Thr Ser

245 250 255

Gly Leu Gln Ile Ala Leu Arg Asp Gly Ala Gln Trp Val Ser Val Pro

260 265 270

Ser Asp Arg Asp Ala Phe Phe Val Asn Val Gly Asp Ser Leu Gln Val

275 280 285

Leu Thr Asn Gly Arg Phe Arg Ser Val Lys His Arg Val Val Thr Asn

290 295 300

Ser Leu Lys Ser Arg Val Ser Phe Ile Tyr Phe Ala Gly Pro Pro Leu

305 310 315 320

Gly Gln Arg Ile Ala Pro Leu Pro Gln Val Leu Ala Glu Gly Glu Glu

325 330 335

Ser Leu Tyr Lys Glu Phe Thr Trp Gly Glu Tyr Lys Lys Ala Ala Tyr

340 345 350

Lys Thr Arg Leu Gly Asp Asn Arg Leu Ala Gln Phe Glu Lys Arg Ser

355 360 365

Asn Ile

370

<210> SEQ ID NO 3

<211> LENGTH: 1650

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 3

caacaccaca gcaacgcacc accggccatc aacctcctct cccctgcttc tcgctcgctc 60

tcctgccctg tttcttggag aagtgagctt aagtgagagc ctctgtccct cgcacacgct 120

attggccatg gtggttctcg ctggcccgcc cgccgtcgat cacatcccgc tgctgaggtc 180

gccggacccc ggcgacgtct tctccggcgt gccggtcgtc gacctcggca gccccggcgc 240

ggcgagggcc gtggtggacg cctgcgagcg gtacgggttc ttcaaggtcg tcaaccacgg 300

cgtggccacg gacacgatgg acaaggccga gtcggaggcc gtcaggttct tctcccagac 360

gcagcccgac aaggaccgct ccggcccggc ctacccgttc gggtacggca gcaagcggat 420

cgggttcaat ggcgacatgg ggtggctcga gtacctcctc ctcgccctcg acgacgcgtc 480

gctcgccgac gcctgcaccg tcccgtcctg cgcggtcttc cgggccgctc tgaacgagta 540

catctcgggg gtgcggaagg tggcggtgcg ggtgatggag gcgatgtcgg aggggctggg 600

cattgcgcag gcggacgcgc tgagcgcgct ggtgacggcg gaagggagcg accaggtgtt 660

ccgcgtgaac cactacccgc cgtgccgcgc gctgcagggg ctcggctgca gcgtcaccgg 720

cttcggcgag cacaccgacc cgcagctcgt ctccgtgctc cgctcaaacg gcacgtccgg 780

cctgcagatc gcgctccgcg acggccagtg ggtgtccgtg ccctccgacc gcgactcctt 840

cttcgtcaac gtcggcgact cgttgcaggt tctgaccaat gggaggttca agagcgtgaa 900

gcacagggtg gtggccaaca gcctaaagtc tagggtttcc ttcatctact ttggagggcc 960

accgttagca cagaggattg caccattgcc acagctgctg ggggagggag agcagagcct 1020

gtacaaggag ttcacatggg atgagtacaa gaaggctgcc tacaaatcaa ggcttggaga 1080

caacaggctg gcccagtttg agaagaagta gctagctaga tgcctagcaa cagaactggc 1140

cggtcaagca acaacgactt tactgcatgg cagctagcta ccttagctat gtctcacgca 1200

cacgtacggt acaccatgac cacgagaaga tgaagctgct taagagagaa aaagaaaaga 1260

aaataaaaac aggaagaaga taataacttc aagatgcaca atgtacagcc aggttggatg 1320

gatagctaga tttttctcgt gtggctaatc accgtgtgtg gtgaggtggg ctcctttcct 1380

gtttcggaat actgatcgat ggatgaacag cctccaatag ttaatgttaa ccaaatatag 1440

cacaacaaag gggttctttt cttcctttgg ttgtttcttt tttcctcttg cctttgattt 1500

tctcgtcatg ctttctgagc attaattatg tgtgcgtgta tttcggtata cgtaacgtac 1560

ttttacctat gtgtgtatag tacgtggctt gtgttaacaa gtaaatatta tagtctctct 1620

ctttcgttaa aaaaaaaaaa aaaaaaaaaa 1650

<210> SEQ ID NO 4

<211> LENGTH: 368

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 4

Thr Thr Ala Thr His His Arg Pro Ser Thr Ser Ser Pro Leu Leu Leu

1 5 10 15

Ala Arg Ser Pro Ala Leu Phe Leu Gly Glu Val Ser Leu Ser Glu Ser

20 25 30

Leu Cys Pro Ser His Thr Leu Leu Ala Met Val Val Leu Ala Gly Pro

›SEQUENCE LISTING · 2 of 53

35 40 45

Pro Ala Val Asp His Ile Pro Leu Leu Arg Ser Pro Asp Pro Gly Asp

50 55 60

Val Phe Ser Gly Val Pro Val Val Asp Leu Gly Ser Pro Gly Ala Ala

65 70 75 80

Arg Ala Val Val Asp Ala Cys Glu Arg Tyr Gly Phe Phe Lys Val Val

85 90 95

Asn His Gly Val Ala Thr Asp Thr Met Asp Lys Ala Glu Ser Glu Ala

100 105 110

Val Arg Phe Phe Ser Gln Thr Gln Pro Asp Lys Asp Arg Ser Gly Pro

115 120 125

Ala Tyr Pro Phe Gly Tyr Gly Ser Lys Arg Ile Gly Phe Asn Gly Asp

130 135 140

Met Gly Trp Leu Glu Tyr Leu Leu Leu Ala Leu Asp Asp Ala Ser Leu

145 150 155 160

Ala Asp Ala Cys Thr Val Pro Ser Cys Ala Val Phe Arg Ala Ala Leu

165 170 175

Asn Glu Tyr Ile Ser Gly Val Arg Lys Val Ala Val Arg Val Met Glu

180 185 190

Ala Met Ser Glu Gly Leu Gly Ile Ala Gln Ala Asp Ala Leu Ser Ala

195 200 205

Leu Val Thr Ala Glu Gly Ser Asp Gln Val Phe Arg Val Asn His Tyr

210 215 220

Pro Pro Cys Arg Ala Leu Gln Gly Leu Gly Cys Ser Val Thr Gly Phe

225 230 235 240

Gly Glu His Thr Asp Pro Gln Leu Val Ser Val Leu Arg Ser Asn Gly

245 250 255

Thr Ser Gly Leu Gln Ile Ala Leu Arg Asp Gly Gln Trp Val Ser Val

260 265 270

Pro Ser Asp Arg Asp Ser Phe Phe Val Asn Val Gly Asp Ser Leu Gln

275 280 285

Val Leu Thr Asn Gly Arg Phe Lys Ser Val Lys His Arg Val Val Ala

290 295 300

Asn Ser Leu Lys Ser Arg Val Ser Phe Ile Tyr Phe Gly Gly Pro Pro

305 310 315 320

Leu Ala Gln Arg Ile Ala Pro Leu Pro Gln Leu Leu Gly Glu Gly Glu

325 330 335

Gln Ser Leu Tyr Lys Glu Phe Thr Trp Asp Glu Tyr Lys Lys Ala Ala

340 345 350

Tyr Lys Ser Arg Leu Gly Asp Asn Arg Leu Ala Gln Phe Glu Lys Lys

355 360 365

<210> SEQ ID NO 5

<211> LENGTH: 1365

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 5

gcacgagctc aaagccaaac caaattttct ctctttcact tatttattga ctccccaaaa 60

gcttttcaaa tatctctgtt cctctctcac gtaccttctc cctatctttc actccacttc 120

ttaacaccac aaaacagaat aacggccgag caaacaacaa gaaaaatggt gttgttgtcc 180

aaagcaacaa cagaacaata ctcctacatt aagaactgca tgccaaccaa attttcctca 240

acaattccca tagtggacct ctccaaacct gatgcaaaga cccttatagt gaaggcttgt 300

gaggagtttg gattcttcaa agtcatcaat catggtgtct ccatggaagc tatatccgaa 360

ttggaatatg aagccttcaa attcttctct atgtcactca atgaaaagga aaaagtagga 420

cctcccaatc catttgggta tggtagcaag aaaattggac acaataggga cgttggttgg 480

attgagtacc ttcttctcaa caccaatcaa gaacacaact tctctgttta tggcaaaaac 540

cctgagaaat tcaggtgtct gttgaacagt tacatgtctt ctgtgaggaa gatggcatgt 600

gagattcttg agttgatggc agaagggttg aagattcagc aaaaggatgt gtttagcaag 660

cttctaatgg ataaacaaag tgactctatt ttcagggtga atcattaccc tgcttgtcct 720

gaaatgactc tgaatgatca gaacttgatt gggtttggag aacacacaga cccacaaatc 780

atctctctgt taagatccaa caacacttca ggccttcaga tttatcttag agatggaaat 840

tggatttcag tcccaccaga tgacaaatcc ttttttatta acgttggtga ttctcttcag 900

gttatgacaa atggaaggtt ccgaagtgtg agacacagag tgttggcaaa tgggttcaag 960

tccaggcttt caatgattta ctttggaggt ccacctttga gtgagaaaat agcaccatta 1020

tcctctctca tgaaaggaaa agaaagtcta tataaagagt ttacctggtt tgagtacaaa 1080

aaatcaatct acggttcaag attatctaaa aatagacttg aacattttga aagaattgca 1140

gcttcgtaat atatattcta gagaattgtt tgagacactt gtcaatacaa aatgggagat 1200

tacaatatgt agtttgtata ctataaatgc tttttttttt ttttttttac ttttaaggtt 1260

atttatcaat cagaaggctt catagacatg ataacatatg tatccatttt gtttaaatgc 1320

tagtgaatga catctaatgt tgttatcaaa aaaaaaaaaa aaaaa 1365

<210> SEQ ID NO 6

<211> LENGTH: 382

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 6

Ala Arg Ala Gln Ser Gln Thr Lys Phe Ser Leu Phe His Leu Phe Ile

1 5 10 15

Asp Ser Pro Lys Ala Phe Gln Ile Ser Leu Phe Leu Ser His Val Pro

20 25 30

Ser Pro Tyr Leu Ser Leu His Phe Leu Thr Pro Gln Asn Arg Ile Thr

35 40 45

Ala Glu Gln Thr Thr Arg Lys Met Val Leu Leu Ser Lys Ala Thr Thr

50 55 60

Glu Gln Tyr Ser Tyr Ile Lys Asn Cys Met Pro Thr Lys Phe Ser Ser

65 70 75 80

Thr Ile Pro Ile Val Asp Leu Ser Lys Pro Asp Ala Lys Thr Leu Ile

85 90 95

Val Lys Ala Cys Glu Glu Phe Gly Phe Phe Lys Val Ile Asn His Gly

100 105 110

Val Ser Met Glu Ala Ile Ser Glu Leu Glu Tyr Glu Ala Phe Lys Phe

115 120 125

Phe Ser Met Ser Leu Asn Glu Lys Glu Lys Val Gly Pro Pro Asn Pro

130 135 140

Phe Gly Tyr Gly Ser Lys Lys Ile Gly His Asn Arg Asp Val Gly Trp

145 150 155 160

Ile Glu Tyr Leu Leu Leu Asn Thr Asn Gln Glu His Asn Phe Ser Val

165 170 175

Tyr Gly Lys Asn Pro Glu Lys Phe Arg Cys Leu Leu Asn Ser Tyr Met

180 185 190

Ser Ser Val Arg Lys Met Ala Cys Glu Ile Leu Glu Leu Met Ala Glu

195 200 205

Gly Leu Lys Ile Gln Gln Lys Asp Val Phe Ser Lys Leu Leu Met Asp

210 215 220

Lys Gln Ser Asp Ser Ile Phe Arg Val Asn His Tyr Pro Ala Cys Pro

225 230 235 240

Glu Met Thr Leu Asn Asp Gln Asn Leu Ile Gly Phe Gly Glu His Thr

245 250 255

Asp Pro Gln Ile Ile Ser Leu Leu Arg Ser Asn Asn Thr Ser Gly Leu

260 265 270

Gln Ile Tyr Leu Arg Asp Gly Asn Trp Ile Ser Val Pro Pro Asp Asp

275 280 285

Lys Ser Phe Phe Ile Asn Val Gly Asp Ser Leu Gln Val Met Thr Asn

290 295 300

Gly Arg Phe Arg Ser Val Arg His Arg Val Leu Ala Asn Gly Phe Lys

305 310 315 320

Ser Arg Leu Ser Met Ile Tyr Phe Gly Gly Pro Pro Leu Ser Glu Lys

325 330 335

Ile Ala Pro Leu Ser Ser Leu Met Lys Gly Lys Glu Ser Leu Tyr Lys

340 345 350

Glu Phe Thr Trp Phe Glu Tyr Lys Lys Ser Ile Tyr Gly Ser Arg Leu

355 360 365

Ser Lys Asn Arg Leu Glu His Phe Glu Arg Ile Ala Ala Ser

370 375 380

<210> SEQ ID NO 7

<211> LENGTH: 1473

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 7

gcacgagagc atctcccctg ttccgcccgc tcgcctctgc ttctttagtt ctttccagag 60

cggctctgct ctgctcctcg cctctctcga tcgcgcatac gtaccatatt tggcagccca 120

gccatggtgg ttctcgccag cacgcccgcc gtcgatcaca tcccgctcct caggtcgccc 180

gaccccgggg actacttctc cggcatgccg gtggtcgacc tctccagccc tggcgcgccg 240

cgggccatcg ccgacgcgtg cgagcgcttc gggttcttca agctcgtcaa ccacggggtg 300

cccgcggaca cgatggacag gctcgagtcg gaggccgtca ggttcttctc gctgccgcag 360

gccgacaagg accgctccgg cccggcctac ccgttcggct acggcagcaa gcgcatcggg 420

ctcaatggcg acatggggtg gctcgagtac ctgctcctgg ccgtcgactc cgcgtcgctc 480

tccggcgcct gcgccgtccc gtcctgcgcg ctcttccggg cggcgctgaa cgagtacatc 540

gcggcggtgc ggaaggtggc ggtgcgggtg atggaggcga tggcggaggg gctgggcatt 600

gcggccgtgg acgcgctgag cgggatggtg acggcggagg ggagcgacca ggtgttccgg 660

gtgaaccact acccgccgtg ccacgcgctg caggggctgg gctgcagcgc caccggcttc 720

ggcgagcaca cggacccgca gctcatctcc gtgctgcgct ccaacggcac gtccggcctg 780

›SEQUENCE LISTING · 3 of 53

cagatcgcgc tccagaacgg gcagtgggtg tccgtgccct cggaccgcga cgccttcttc 840

gtcaacgtcg gcgactcgtt gcaggtgctg accaacggga ggttcaagag cgtgaagcac 900

agggtggtgg ccaacagcct aaagtctagg gtttccatga tctactttgg agggccagcg 960

atgacacaga ggattgcacc attgccgcag ctgctgggcg cgggagagca gagcctgtac 1020

aaggacttca catggggcga gtacaagaag gctgcctaca actccaggct cggggacaac 1080

aggctggctc agttccacag gtagcatcgc ctgccgcctg ggcccgctca aggagcagct 1140

tttaccaagc cagccagcta gctagtagct agctagctgc cgtggcttat gtttcgcaca 1200

ccgtgaagac gaagatgaag atgaagagga aagctactag aaaagaaagg gaaacagagc 1260

taaaagaaga tgatttcaag acacatgtgg tagagctgcg gacgtagcta gtagatcttt 1320

ttcgtgtgcg tgtgcgcgtg tgtgtgcgtg tgcgtgtgtg ttcatagttg ctaaaactcg 1380

cctttcctat tcgggaatgc cggacgaacc atgaacagat aagttaactt gatgaatagt 1440

tagttagtgt gaaccaaaaa aaaaaaaaaa aaa 1473

<210> SEQ ID NO 8

<211> LENGTH: 351

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 8

Phe Phe Pro Glu Arg Leu Cys Ser Ala Pro Arg Leu Ser Arg Ser Arg

1 5 10 15

Ile Arg Thr Ile Phe Gly Ser Pro Ala Met Val Val Leu Ala Ser Thr

20 25 30

Pro Ala Val Asp His Ile Pro Leu Leu Arg Ser Pro Asp Pro Gly Asp

35 40 45

Tyr Phe Ser Gly Met Pro Val Val Asp Leu Ser Ser Pro Gly Ala Pro

50 55 60

Arg Ala Ile Ala Asp Ala Cys Glu Arg Phe Gly Phe Phe Lys Leu Val

65 70 75 80

Asn His Gly Val Pro Ala Asp Thr Met Asp Arg Leu Glu Ser Glu Ala

85 90 95

Val Arg Phe Phe Ser Leu Pro Gln Ala Asp Lys Asp Arg Ser Gly Pro

100 105 110

Ala Tyr Pro Phe Gly Tyr Gly Ser Lys Arg Ile Gly Leu Asn Gly Asp

115 120 125

Met Gly Trp Leu Glu Tyr Leu Leu Leu Ala Val Asp Ser Ala Ser Leu

130 135 140

Ser Gly Ala Cys Ala Val Pro Ser Cys Ala Leu Phe Arg Ala Ala Leu

145 150 155 160

Asn Glu Tyr Ile Ala Ala Val Arg Lys Val Ala Val Arg Val Met Glu

165 170 175

Ala Met Ala Glu Gly Leu Gly Ile Ala Ala Val Asp Ala Leu Ser Gly

180 185 190

Met Val Thr Ala Glu Gly Ser Asp Gln Val Phe Arg Val Asn His Tyr

195 200 205

Pro Pro Cys His Ala Leu Gln Gly Leu Gly Cys Ser Ala Thr Gly Phe

210 215 220

Gly Glu His Thr Asp Pro Gln Leu Ile Ser Val Leu Arg Ser Asn Gly

225 230 235 240

Thr Ser Gly Leu Gln Ile Ala Leu Gln Asn Gly Gln Trp Val Ser Val

245 250 255

Pro Ser Asp Arg Asp Ala Phe Phe Val Asn Val Gly Asp Ser Leu Gln

260 265 270

Val Leu Thr Asn Gly Arg Phe Lys Ser Val Lys His Arg Val Val Ala

275 280 285

Asn Ser Leu Lys Ser Arg Val Ser Met Ile Tyr Phe Gly Gly Pro Ala

290 295 300

Met Thr Gln Arg Ile Ala Pro Leu Pro Gln Leu Leu Gly Ala Gly Glu

305 310 315 320

Gln Ser Leu Tyr Lys Asp Phe Thr Trp Gly Glu Tyr Lys Lys Ala Ala

325 330 335

Tyr Asn Ser Arg Leu Gly Asp Asn Arg Leu Ala Gln Phe His Arg

340 345 350

<210> SEQ ID NO 9

<211> LENGTH: 450

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (439)

<400> SEQUENCE: 9

gactcgactc ccacctcccc tgttctcacc gtgcttgccc tgtttccacc tctgctgccc 60

atccgcctcg cttgcccgcc gtacagtaca ggccagacag ccatggtggt gctcgccaaa 120

ccgcctgtcg tcgaccagat cccgctcctg cggtccccgg gccccaggga cagcttctcg 180

ggagtgccgg tcgtcgacct gtccagccac ggcgcggcgc gggcgatcgt cgacgcctgc 240

gagcgcttcg ggttcttcaa ggtcgtcaac cacggcgtgg ccgcggccac catggacagg 300

gccgagtccg aggccgtcag gttcttcgcg caggcgcagg cggacaagga ccgcgcgggg 360

ccggcgtacc cgttcgggta cggcagcaag cggatcgggc tcaatggcga catggggtgg 420

ctcgagtacc tctcctcgnc gtcgacgccc 450

<210> SEQ ID NO 10

<211> LENGTH: 110

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 10

Met Val Val Leu Ala Lys Pro Pro Val Val Asp Gln Ile Pro Leu Leu

1 5 10 15

Arg Ser Pro Gly Pro Arg Asp Ser Phe Ser Gly Val Pro Val Val Asp

20 25 30

Leu Ser Ser His Gly Ala Ala Arg Ala Ile Val Asp Ala Cys Glu Arg

35 40 45

Phe Gly Phe Phe Lys Val Val Asn His Gly Val Ala Ala Ala Thr Met

50 55 60

Asp Arg Ala Glu Ser Glu Ala Val Arg Phe Phe Ala Gln Ala Gln Ala

65 70 75 80

Asp Lys Asp Arg Ala Gly Pro Ala Tyr Pro Phe Gly Tyr Gly Ser Lys

85 90 95

Arg Ile Gly Leu Asn Gly Asp Met Gly Trp Leu Glu Tyr Leu

100 105 110

<210> SEQ ID NO 11

<211> LENGTH: 618

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (482)

<221> NAME/KEY: unsure

<222> LOCATION: (531)

<221> NAME/KEY: unsure

<222> LOCATION: (579)

<221> NAME/KEY: unsure

<222> LOCATION: (616)

<400> SEQUENCE: 11

acaccacagc aacgcaccac cggccatcaa cctcctctcc cctgcttctc gctcgctctc 60

ctgccctgtt tcttggagaa gtgagcttaa gtgagagcct ctgtccctcg cacacgctat 120

tggccatggt ggttctcgct ggcccgcccg ccgtcgatca catcccgctg ctgaggtcgc 180

cggaccccgg cgacgtcttc tccggcgtgc cggtcgtcga cctcggcagc cccggcgcgg 240

cgagggccgt ggtggacgcc tgcgagcggt acgggttctt caaggtcgtc aaccacggcg 300

tggccacgga cacgatggac aaggccgatc ggaggccgtc aggttcttct cccaagacgc 360

agcccgacaa ggaccgctcc ggcccggcta ccgttcgggt acggcaacaa gcggatcggg 420

ttcaatggcg acatgggtgg tcgagtactc ctctcgccct cgacgaccgt cgctcgccga 480

cnctgcacgt cccttctgcg cgtcttccgg gccctctgaa caataatctc ngggttccga 540

agtggcgtcc ggtgatggag caattcgagg ggtgggatnc caagcgaccc ctaacccctg 600

tacgcgaagg acacantt 618

<210> SEQ ID NO 12

<211> LENGTH: 105

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (88)..(89)

<400> SEQUENCE: 12

Met Val Val Leu Ala Gly Pro Pro Ala Val Asp His Ile Pro Leu Leu

1 5 10 15

Arg Ser Pro Asp Pro Gly Asp Val Phe Ser Gly Val Pro Val Val Asp

20 25 30

Leu Gly Ser Pro Gly Ala Ala Arg Ala Val Val Asp Ala Cys Glu Arg

35 40 45

Tyr Gly Phe Phe Lys Val Val Asn His Gly Val Ala Thr Asp Thr Met

50 55 60

Asp Lys Ala Asp Arg Arg Pro Ser Gly Ser Ser Pro Lys Thr Gln Pro

65 70 75 80

Asp Lys Asp Arg Ser Gly Pro Xaa Xaa Pro Phe Gly Tyr Gly Asn Lys

85 90 95

Arg Ile Gly Phe Asn Gly Asp Met Gly

100 105

<210> SEQ ID NO 13

<211> LENGTH: 1352

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 13

ctcaaagcca aaccaaattt tctctctttc acttatttat tgactcccca aaagcttttc 60

aaatatctct gttcctctct cacgtacctt ctccctatct ttcactccac ttcttaacac 120

cacaaaacag aataacggcc gagcaaacaa caagaaaaat ggtgttgttg tccaaagcaa 180

caacagaaca atactcctac attaagaact gcatgccaac caaattttcc tcaacaattc 240

ccatagtgga cctctccaaa cctgatgcaa agacccttat agtgaaggct tgtgaggagt 300

ttggattctt caaagtcatc aatcatggtg tctccatgga agctatatcc gaattggaat 360

atgaagcctt caaattcttc tctatgtcac tcaatgaaaa ggaaaaagta ggacctccca 420

atccatttgg gtatggtagc aagaaaattg gacacaatgg ggacgttggt tggattgagt 480

›SEQUENCE LISTING · 4 of 53

accttcttct caacaccaat caagaacaca acttctctgt ttatgggaaa aaccctgaga 540

aattcaggtg tctgttgaac agttacatgt cttctgtgag gaagatggca tgtgagattc 600

ttgagttgat ggcagaaggg ttgaagattc agcaaaagga tgtgtttagc aagcttctaa 660

tggataaaca aagtgactct attttcaggg tgaatcatta ccctgcttgt cctgaaatga 720

ctctgaatga tcagaacttg attgggtttg gagaacacac agacccacaa atcatctctc 780

tgttaagatc caacaacact tcaggccttc agatttatct tagagatgga aattggattt 840

cagtcccacc agatgacaaa tcctttttta ttaacgttgg tgattctctt caggttatga 900

caaatggaag gttccgaagt gtgagacaca gagtgttggc aaatgggttc aagtccaggc 960

tttcaatgat ttactttgga ggtccacctt tgagtgagaa aatagcacca ttatcctctc 1020

tcatgaaagg aaaagaaagt ctatataaag agtttacctg gtttgagtac aaaaaatcaa 1080

tctacggttc aagattatct aaaaatagac ttgaacattt tgaaagaatt gcagcttcgt 1140

aatatatatt ctagagaatt gtttgagaca cttgtcaata caaaatggga gattacaata 1200

tgtagtttgt atactataaa tgcttttttt tttttttttt tacttttaag gttatttatc 1260

aatcaagaag gcttcataga catgataaca tatgtatcca ttttgtttaa atgctagtga 1320

atgacatcta atgttgttat caaaaaaaaa aa 1352

<210> SEQ ID NO 14

<211> LENGTH: 327

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 14

Met Val Leu Leu Ser Lys Ala Thr Thr Glu Gln Tyr Ser Tyr Ile Lys

1 5 10 15

Asn Cys Met Pro Thr Lys Phe Ser Ser Thr Ile Pro Ile Val Asp Leu

20 25 30

Ser Lys Pro Asp Ala Lys Thr Leu Ile Val Lys Ala Cys Glu Glu Phe

35 40 45

Gly Phe Phe Lys Val Ile Asn His Gly Val Ser Met Glu Ala Ile Ser

50 55 60

Glu Leu Glu Tyr Glu Ala Phe Lys Phe Phe Ser Met Ser Leu Asn Glu

65 70 75 80

Lys Glu Lys Val Gly Pro Pro Asn Pro Phe Gly Tyr Gly Ser Lys Lys

85 90 95

Ile Gly His Asn Gly Asp Val Gly Trp Ile Glu Tyr Leu Leu Leu Asn

100 105 110

Thr Asn Gln Glu His Asn Phe Ser Val Tyr Gly Lys Asn Pro Glu Lys

115 120 125

Phe Arg Cys Leu Leu Asn Ser Tyr Met Ser Ser Val Arg Lys Met Ala

130 135 140

Cys Glu Ile Leu Glu Leu Met Ala Glu Gly Leu Lys Ile Gln Gln Lys

145 150 155 160

Asp Val Phe Ser Lys Leu Leu Met Asp Lys Gln Ser Asp Ser Ile Phe

165 170 175

Arg Val Asn His Tyr Pro Ala Cys Pro Glu Met Thr Leu Asn Asp Gln

180 185 190

Asn Leu Ile Gly Phe Gly Glu His Thr Asp Pro Gln Ile Ile Ser Leu

195 200 205

Leu Arg Ser Asn Asn Thr Ser Gly Leu Gln Ile Tyr Leu Arg Asp Gly

210 215 220

Asn Trp Ile Ser Val Pro Pro Asp Asp Lys Ser Phe Phe Ile Asn Val

225 230 235 240

Gly Asp Ser Leu Gln Val Met Thr Asn Gly Arg Phe Arg Ser Val Arg

245 250 255

His Arg Val Leu Ala Asn Gly Phe Lys Ser Arg Leu Ser Met Ile Tyr

260 265 270

Phe Gly Gly Pro Pro Leu Ser Glu Lys Ile Ala Pro Leu Ser Ser Leu

275 280 285

Met Lys Gly Lys Glu Ser Leu Tyr Lys Glu Phe Thr Trp Phe Glu Tyr

290 295 300

Lys Lys Ser Ile Tyr Gly Ser Arg Leu Ser Lys Asn Arg Leu Glu His

305 310 315 320

Phe Glu Arg Ile Ala Ala Ser

325

<210> SEQ ID NO 15

<211> LENGTH: 595

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (451)

<221> NAME/KEY: unsure

<222> LOCATION: (467)

<221> NAME/KEY: unsure

<222> LOCATION: (486)

<221> NAME/KEY: unsure

<222> LOCATION: (506)

<221> NAME/KEY: unsure

<222> LOCATION: (510)

<221> NAME/KEY: unsure

<222> LOCATION: (559)

<221> NAME/KEY: unsure

<222> LOCATION: (564)

<221> NAME/KEY: unsure

<222> LOCATION: (571)..(572)

<221> NAME/KEY: unsure

<222> LOCATION: (578)

<221> NAME/KEY: unsure

<222> LOCATION: (582)

<221> NAME/KEY: unsure

<222> LOCATION: (591)..(592)

<400> SEQUENCE: 15

agcatctccc ctgttccgcc cgctcgcctc tgcttcttta gttctttcca gagcggctct 60

gctctgctcc tcgcctctct cgatcgcgca tacgtaccat atttggcagc ccagccatgg 120

tggttctcgc cagcacgccc gccgtcgatc acatcccgct cctcaggtcg cccgaccccg 180

gggactactt ctccggcatg ccggtggtcg acctctccag ccctggcgcg ccgcgggcca 240

tcgccgacgc gtgcgagcgc ttcgggttct tcaagctcgt caaccacggg gtgcccgcgg 300

acacgatgga caggctcgag tcggaggccg tcaggttctt ctcgctgccg caagccgaca 360

aggaccgctc cggccgggct accgttcggt acggaacaag cgcacgggct catggcgaca 420

tggggtggtc gagtacctgt ctggccgtca ntccgcgtcg tctcggngct gcgcgtccgt 480

ctgggngctc ttcgggcggg gtgaanatan acgcggggtc ggagtgcgtt cggtatgaag 540

catgcgaggg ctggaattng ctgnacctaa nngaatgnaa gngggggaac nnaat 595

<210> SEQ ID NO 16

<211> LENGTH: 86

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 16

Met Val Val Leu Ala Ser Thr Pro Ala Val Asp His Ile Pro Leu Leu

1 5 10 15

Arg Ser Pro Asp Pro Gly Asp Tyr Phe Ser Gly Met Pro Val Val Asp

20 25 30

Leu Ser Ser Pro Gly Ala Pro Arg Ala Ile Ala Asp Ala Cys Glu Arg

35 40 45

Phe Gly Phe Phe Lys Leu Val Asn His Gly Val Pro Ala Asp Thr Met

50 55 60

Asp Arg Leu Glu Ser Glu Ala Val Arg Phe Phe Ser Leu Pro Gln Ala

65 70 75 80

Asp Lys Asp Arg Ser Gly

85

<210> SEQ ID NO 17

<211> LENGTH: 682

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 17

gcacgagctt acagacattc agtagaacag aagacataac atttgcaacc gaggaatgcc 60

tccttcttac tatagcataa agcgatggga atctatcttt agaggtactg tttgattgag 120

gttgtataca actaattaac actacgacaa acgtacgtat ttatagagac caaaaactat 180

gtgtaaaatt agtaatttaa tgtctgtcga ttgattgtat tgatcaatgt cgacacgtag 240

attggttacg ccctagactt cccctggaaa gcaagtctgc cacgtgttga aacaagaatg 300

tatctggagc aatatggcgc cggcagagat gtgtggatcg gtaagtcgct ttacaggctg 360

ccactcgtga acaatgatct ctatcacaac atggcaaaag ctgatttcaa gtgtttccag 420

aggcgctgcc aacttgagtg gcatggcatc aacaagtgag ctgcctcctg cctctccatc 480

cgactgatct actttaattt ggaatggaaa taaacaaaga attactacgt actaccccct 540

cctaaaatag ttgtgtttat aggattaaaa ttgtcctaaa atagtttaaa tctactttgc 600

aattttttaa actattgtct cattaattac attatattca aatttctctt ctttttaaaa 660

aaaaaaaaaa aaaaaaaaaa aa 682

<210> SEQ ID NO 18

<211> LENGTH: 72

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 18

Ile Gly Tyr Ala Leu Asp Phe Pro Trp Lys Ala Ser Leu Pro Arg Val

1 5 10 15

Glu Thr Arg Met Tyr Leu Glu Gln Tyr Gly Ala Gly Arg Asp Val Trp

20 25 30

Ile Gly Lys Ser Leu Tyr Arg Leu Pro Leu Val Asn Asn Asp Leu Tyr

35 40 45

His Asn Met Ala Lys Ala Asp Phe Lys Cys Phe Gln Arg Arg Cys Gln

50 55 60

Leu Glu Trp His Gly Ile Asn Lys

65 70

<210> SEQ ID NO 19

<211> LENGTH: 497

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (270)

<221> NAME/KEY: unsure

<222> LOCATION: (335)

<221> NAME/KEY: unsure

<222> LOCATION: (443)

<221> NAME/KEY: unsure

<222> LOCATION: (456)

<400> SEQUENCE: 19

tacgacaaaa caagattacg ctcatatggc catggaggcc agtgaatacc tcgatggaat 60

›SEQUENCE LISTING · 5 of 53

cgtcaaaaag ttcaatggag gagttccctg tatctaccct ttggatgtgt acgaacgctt 120

atgggccgtc gataggctga cgaggctggg catatcaagg cacttcacaa gtgaaattga 180

ggattgctta gactacattt tcaggaactg gactccagat ggattagctc acacaaagaa 240

ctgcccggta aaggatatcg atgacacggn catgggtttc cgtctcctcc gactttacgg 300

ataccaagtc gacccatgtg tgttgaataa gttcnaaaag gatggcaagt tcttctgctt 360

gcacggggag tccaacccat cctctgtcac cccaatgtac aacacttacc gggcctccca 420

actcaaattt cctggcgatg acngtgtcct tgggcnaact gaggtgtttt gccgctcaat 480

cctccaaaga caaggag 497

<210> SEQ ID NO 20

<211> LENGTH: 139

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (82)

<221> NAME/KEY: UNSURE

<222> LOCATION: (104)

<400> SEQUENCE: 20

Met Ala Met Glu Ala Ser Glu Tyr Leu Asp Gly Ile Val Lys Lys Phe

1 5 10 15

Asn Gly Gly Val Pro Cys Ile Tyr Pro Leu Asp Val Tyr Glu Arg Leu

20 25 30

Trp Ala Val Asp Arg Leu Thr Arg Leu Gly Ile Ser Arg His Phe Thr

35 40 45

Ser Glu Ile Glu Asp Cys Leu Asp Tyr Ile Phe Arg Asn Trp Thr Pro

50 55 60

Asp Gly Leu Ala His Thr Lys Asn Cys Pro Val Lys Asp Ile Asp Asp

65 70 75 80

Thr Xaa Met Gly Phe Arg Leu Leu Arg Leu Tyr Gly Tyr Gln Val Asp

85 90 95

Pro Cys Val Leu Asn Lys Phe Xaa Lys Asp Gly Lys Phe Phe Cys Leu

100 105 110

His Gly Glu Ser Asn Pro Ser Ser Val Thr Pro Met Tyr Asn Thr Tyr

115 120 125

Arg Ala Ser Gln Leu Lys Phe Pro Gly Asp Asp

130 135

<210> SEQ ID NO 21

<211> LENGTH: 2543

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 21

gcacgaggcc accttccctc tttctcttct tctaaccatt tccctttttc atcttcttct 60

tctatctctc ttcaccactt ctccaaatcc tctctgggtg ccgtgttgtc cgagactaac 120

gacaaacaag agataagatg cagagcaata tccaaaccac gaacacaaga atattcagac 180

atatttcagg gtagtctagc aacgttaaag tttcgtgaga ttaatgtgga agatgacata 240

gaagaggagc aagacatcgg gaaggttttt gtggcaaatg agatcaagaa gagagtggac 300

actatcaaat caatattggg ttccatggaa gatggagaga taacagtatc tgcttatgac 360

actgcttggg ttgctctgat agaagatgtt catggaagtg gtgctcctca atttccatcg 420

agtctagaat ggatagcgaa aaatcaacac cctgatggtt catggggtga taaggaatta 480

ttctcagccc atgatcggat tattaacacg ttggcttgtg ttattgcatt aaaatcatgg 540

catatgcacc ctgaaaagtg tgagaaagga atgacatttt ttaaggagaa tcttaaccag 600

cttcagaatg agaatgtgga gcatatgcca attgggtttg aagtagcttt tccatcactt 660

cttgacatgg ctcgtggctt gaacattgaa gtgccggaca attccccaat cctgaacaaa 720

atcttcgcaa tgagaaacgt aaaactcaca agaataccaa aagcgatgat gcataaagtg 780

cccacaagtc tgcttcatag cttggaaggg atgtcaggcc ttgactggaa agagcttcta 840

aaactgcagt ctcaagacgg gtccttcttg ttttctccgt cctccactgc ctttgctctc 900

atgcaaacta aagaccaaaa ttgccacaat tacttgaata aagtggtcaa gaggttcaat 960

gggggagttc cgaacgtgta cccagtggat ttgttcgaac atatttgggt ggttgatagg 1020

cttgaacgcc tcggaatatc tcagtatttt cagcaagaga tcaaggactg tttgagttat 1080

gttcacagat attggactga aaagggtatt tgttgggcaa gaaattcaaa tgttcaagac 1140

attgatgaca cggcaatggg tttcagatta ctaagattac acggctatca agtttcagcc 1200

gatgtgttca agaactttga gagaaatggt gaatttttct gcttcacggg gcagactaca 1260

caagccgtga caggaatgtt taatctatat agggccacac aagtaatgtt cccgggagag 1320

aaaattcttg agcatgggaa gcatttctct gccaaatttt tgagggataa gagagcagca 1380

aatgagctag tacataaatg gatcataatg aagaacctgg cagaagaggt tgcgtacgct 1440

ttggacgtac catggtatgc aagcctacct cgagtggaga caagattcta cattgatcaa 1500

tacggtggtg agagtgacgt gtggataggc aaaacccttt acaggatggc ctatgtgaac 1560

aacaataact atctcgagct tgctaaatta gattacaaca attgccaggc actgcatcta 1620

atagagtggg ggagaattca gaagtggtac tcagaatcta ggttgggaga gtttggattg 1680

aacagaagaa ctcttctgtt ggcctatttt ctggcagcag caagcatatt tgagcctgaa 1740

aagtctcatg tgagactagc gtgggccaaa accagtgtct tacttgagac cataacatcc 1800

tatgttagtg atgcagaaat gaggaaagat ttcatgaaaa aattcagtga ctgcattaac 1860

aggcgagact actccatggg ctggaggttg aacaggaaca gaataggaca tggactggct 1920

gaggctttgg ttgccaccat agatcaaatc tcgtgggata tactcgtgtc tcacggtcac 1980

gaaattggat atcacatgca tcgcagttgg gaaaagtggc tttcaagttg gcatcgcgaa 2040

ggagacaaat gtgaaggaca agctgagctt ttggcgcaga taataaacct atgtgacggg 2100

cattggattt ccgaggatca agtgttcgat ccacaatatc agagtctcct tcaactcact 2160

aacacactct gcaatagact ccgttgccac caaaaggaca aggaactcga gagcggcaac 2220

tgcggcacga atgtaaacag catgatcacc caagaagaag agtcaaaaat gcaagaactc 2280

gtgcaattgg tgtaccaaaa atctccaact ggcattgatt tcaatatcaa gaatactttc 2340

ctcacagtgg ccaagagttt ttactataca gctttctgtg attcaaggac cgtcaacttc 2400

catattgcca aagttctgtt tgataaagtc gtttaaatca aattttattt cttgaataga 2460

tattagactc caccatatca ttgtgaatat tatccctctg taatgataaa aagaaaggaa 2520

aacaaaaaaa aaaaaaaaaa aaa 2543

<210> SEQ ID NO 22

<211> LENGTH: 811

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 22

Ala Arg Gly His Leu Pro Ser Phe Ser Ser Ser Asn His Phe Pro Phe

1 5 10 15

Ser Ser Ser Ser Ser Ile Ser Leu His His Phe Ser Lys Ser Ser Leu

20 25 30

Gly Ala Val Leu Ser Glu Thr Asn Asp Lys Gln Glu Ile Arg Cys Arg

35 40 45

Ala Ile Ser Lys Pro Arg Thr Gln Glu Tyr Ser Asp Ile Phe Gln Gly

50 55 60

Ser Leu Ala Thr Leu Lys Phe Arg Glu Ile Asn Val Glu Asp Asp Ile

65 70 75 80

Glu Glu Glu Gln Asp Ile Gly Lys Val Phe Val Ala Asn Glu Ile Lys

85 90 95

Lys Arg Val Asp Thr Ile Lys Ser Ile Leu Gly Ser Met Glu Asp Gly

100 105 110

Glu Ile Thr Val Ser Ala Tyr Asp Thr Ala Trp Val Ala Leu Ile Glu

115 120 125

Asp Val His Gly Ser Gly Ala Pro Gln Phe Pro Ser Ser Leu Glu Trp

130 135 140

Ile Ala Lys Asn Gln His Pro Asp Gly Ser Trp Gly Asp Lys Glu Leu

145 150 155 160

Phe Ser Ala His Asp Arg Ile Ile Asn Thr Leu Ala Cys Val Ile Ala

165 170 175

Leu Lys Ser Trp His Met His Pro Glu Lys Cys Glu Lys Gly Met Thr

180 185 190

Phe Phe Lys Glu Asn Leu Asn Gln Leu Gln Asn Glu Asn Val Glu His

195 200 205

Met Pro Ile Gly Phe Glu Val Ala Phe Pro Ser Leu Leu Asp Met Ala

210 215 220

Arg Gly Leu Asn Ile Glu Val Pro Asp Asn Ser Pro Ile Leu Asn Lys

225 230 235 240

Ile Phe Ala Met Arg Asn Val Lys Leu Thr Arg Ile Pro Lys Ala Met

245 250 255

Met His Lys Val Pro Thr Ser Leu Leu His Ser Leu Glu Gly Met Ser

260 265 270

Gly Leu Asp Trp Lys Glu Leu Leu Lys Leu Gln Ser Gln Asp Gly Ser

275 280 285

Phe Leu Phe Ser Pro Ser Ser Thr Ala Phe Ala Leu Met Gln Thr Lys

290 295 300

Asp Gln Asn Cys His Asn Tyr Leu Asn Lys Val Val Lys Arg Phe Asn

305 310 315 320

Gly Gly Val Pro Asn Val Tyr Pro Val Asp Leu Phe Glu His Ile Trp

›SEQUENCE LISTING · 6 of 53

325 330 335

Val Val Asp Arg Leu Glu Arg Leu Gly Ile Ser Gln Tyr Phe Gln Gln

340 345 350

Glu Ile Lys Asp Cys Leu Ser Tyr Val His Arg Tyr Trp Thr Glu Lys

355 360 365

Gly Ile Cys Trp Ala Arg Asn Ser Asn Val Gln Asp Ile Asp Asp Thr

370 375 380

Ala Met Gly Phe Arg Leu Leu Arg Leu His Gly Tyr Gln Val Ser Ala

385 390 395 400

Asp Val Phe Lys Asn Phe Glu Arg Asn Gly Glu Phe Phe Cys Phe Thr

405 410 415

Gly Gln Thr Thr Gln Ala Val Thr Gly Met Phe Asn Leu Tyr Arg Ala

420 425 430

Thr Gln Val Met Phe Pro Gly Glu Lys Ile Leu Glu His Gly Lys His

435 440 445

Phe Ser Ala Lys Phe Leu Arg Asp Lys Arg Ala Ala Asn Glu Leu Val

450 455 460

His Lys Trp Ile Ile Met Lys Asn Leu Ala Glu Glu Val Ala Tyr Ala

465 470 475 480

Leu Asp Val Pro Trp Tyr Ala Ser Leu Pro Arg Val Glu Thr Arg Phe

485 490 495

Tyr Ile Asp Gln Tyr Gly Gly Glu Ser Asp Val Trp Ile Gly Lys Thr

500 505 510

Leu Tyr Arg Met Ala Tyr Val Asn Asn Asn Asn Tyr Leu Glu Leu Ala

515 520 525

Lys Leu Asp Tyr Asn Asn Cys Gln Ala Leu His Leu Ile Glu Trp Gly

530 535 540

Arg Ile Gln Lys Trp Tyr Ser Glu Ser Arg Leu Gly Glu Phe Gly Leu

545 550 555 560

Asn Arg Arg Thr Leu Leu Leu Ala Tyr Phe Leu Ala Ala Ala Ser Ile

565 570 575

Phe Glu Pro Glu Lys Ser His Val Arg Leu Ala Trp Ala Lys Thr Ser

580 585 590

Val Leu Leu Glu Thr Ile Thr Ser Tyr Val Ser Asp Ala Glu Met Arg

595 600 605

Lys Asp Phe Met Lys Lys Phe Ser Asp Cys Ile Asn Arg Arg Asp Tyr

610 615 620

Ser Met Gly Trp Arg Leu Asn Arg Asn Arg Ile Gly His Gly Leu Ala

625 630 635 640

Glu Ala Leu Val Ala Thr Ile Asp Gln Ile Ser Trp Asp Ile Leu Val

645 650 655

Ser His Gly His Glu Ile Gly Tyr His Met His Arg Ser Trp Glu Lys

660 665 670

Trp Leu Ser Ser Trp His Arg Glu Gly Asp Lys Cys Glu Gly Gln Ala

675 680 685

Glu Leu Leu Ala Gln Ile Ile Asn Leu Cys Asp Gly His Trp Ile Ser

690 695 700

Glu Asp Gln Val Phe Asp Pro Gln Tyr Gln Ser Leu Leu Gln Leu Thr

705 710 715 720

Asn Thr Leu Cys Asn Arg Leu Arg Cys His Gln Lys Asp Lys Glu Leu

725 730 735

Glu Ser Gly Asn Cys Gly Thr Asn Val Asn Ser Met Ile Thr Gln Glu

740 745 750

Glu Glu Ser Lys Met Gln Glu Leu Val Gln Leu Val Tyr Gln Lys Ser

755 760 765

Pro Thr Gly Ile Asp Phe Asn Ile Lys Asn Thr Phe Leu Thr Val Ala

770 775 780

Lys Ser Phe Tyr Tyr Thr Ala Phe Cys Asp Ser Arg Thr Val Asn Phe

785 790 795 800

His Ile Ala Lys Val Leu Phe Asp Lys Val Val

805 810

<210> SEQ ID NO 23

<211> LENGTH: 2403

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (1049)

<400> SEQUENCE: 23

ccacgcgtcc gctgcagaac cagcaggatg atggatcttg gggtgtcagc caatctgact 60

catcggtcag caaggatgtt ctcctatcca cgttggcatg tgttcttgcg ttgaagagat 120

ggaatgttgg cagagagaac atttggagag gactgcattt catcgggagg aatttctctg 180

ttgctatgga cgagcagttc acttctccta taggtttcaa cttcaccttt cctggtttgc 240

ttagcctcgg cattgatatg ggtttagaat ttcctgtaag acaaattgat gtctgtggca 300

ttcttcaccg ccgggagatg gaattgaaaa ggctggctgt ggatagttct tttggaagaa 360

aagcatatat ggcttttatc ccagaaggat tcggaaatat gctggactgg gatcaagtta 420

tgaagtttca gaggaagaat ggatcattgt tcagcactcc ttccacaact gctgttgcat 480

taatccacaa atacaacgac caagcccttc aatacctaaa tttgcttgtc aatgaatttg 540

gcagtgcagt accagcaatg tatccttcaa gggtacattg tcagctttca atggtggacg 600

cgcttgaaaa aatgggaatt tctcagcgct ttgtcagtga aatagaaagc atcctggaca 660

tggcatacaa ttgctggtta cagaatgatg aggaactcat gatggacata gcaacatttg 720

caatggcatt tcgccttttg aggatgaatg gttacgatgt ttcctcagat gagctgtctc 780

acgttgctgg agcttccact ttccatgatt cactacaagg atatttaaat gatacaaaat 840

ccctactgga attgtacaag acctcaaaag tcaccttatc agaaaacgat ctgatcttag 900

atcgcatagg ttcctggtct ggcaacttat tgaaggataa gatgtgctgt agtaaggtgc 960

aaaagactcg atttttggag agatgctgca aacaaaattt aaattctcat tttcaccttg 1020

gaggttcagt ttatgtctgt gttttgtgnt ttcagatcga gtatgctgtt aattttccct 1080

tgtattccac actggagcgt ctagaacaca agagaaacat cgaacatttt gatgcttggg 1140

gttctctgat gctaacaaca aaatcctcat cttttcgtat caatcaagaa ttcctagctt 1200

tggcagtcga agatttcagt ttctctcaac gtgtttaccg ggatgaactt cggcatcttg 1260

atagttgggt gaaggagaac aagctggacc agctacaatt tgctcggcag aaactgacat 1320

attgctatct gtctgctgct gctaccgtat tttcttctga attgtctgac gctcgcattt 1380

catgggccaa aaatggtgtc ctcacaactg tggttgatga cttcttcgat gttggtggat 1440

caaaagaaga attagaaaac ctgatagcac tagttgagaa atggcatggg caccatgcag 1500

ttgagttcta ttcggaacag gtgaaaatag tattttctgc tatttataca acagtgaacc 1560

atcttggagc aatggcttct gcagcacaag gccgtgatct tacaaaccac ctagtagaaa 1620

tatggctgga tttgttaaga tctatgatgg tcgaggcaga atggcagaga tgccaatatg 1680

taccaacagt tgaagaatac atgacaaatg ctgttgtctc atttgcactg ggcccaattg 1740

tgctcccagc attgtatttt gtagggcaag agctattaga gcatgctgtc aaagatgaag 1800

agtacgataa attatttagg ctagtgagca cttgcgggag gctcctcaat gactaccaaa 1860

gtttagagag ggaaggcaac caggggaagc tgaatagtgt ttctctactt gtgctccaca 1920

gtggtggttc tatgtccata gaagccgcta aaaaggcaat gcagaagtcc atagacgtgt 1980

ctaggagaga cttgctaaga ttggttctca ggaaagaaag tgctgttcct aggccatgca 2040

aggagctctt ctggaagatg tgtaagatac ttcacctgtt ttactctcag aatgatggat 2100

ttagctcccc aaaggaaatg gtcagtgcag tgaatgctgt tatcaacgag ccactcaaag 2160

tccaaaacag tactacgttt ttgtctagtt catcaaggta gcgaggatat catgtaattt 2220

atcctgttca tatggatcag aagcaacagc agcaagctta agatatttgt aagtttgtga 2280

ctgggctgct gtggatctgt gaagctacaa ctttgctgta tgtggatcaa ggtattagga 2340

agatctggtt cacatattga taacataatg gataaaccat aacgttaaaa aaaaaaaaaa 2400

aag 2403

<210> SEQ ID NO 24

<211> LENGTH: 732

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (349)

<400> SEQUENCE: 24

Thr Arg Pro Leu Gln Asn Gln Gln Asp Asp Gly Ser Trp Gly Val Ser

1 5 10 15

Gln Ser Asp Ser Ser Val Ser Lys Asp Val Leu Leu Ser Thr Leu Ala

20 25 30

Cys Val Leu Ala Leu Lys Arg Trp Asn Val Gly Arg Glu Asn Ile Trp

35 40 45

Arg Gly Leu His Phe Ile Gly Arg Asn Phe Ser Val Ala Met Asp Glu

50 55 60

Gln Phe Thr Ser Pro Ile Gly Phe Asn Phe Thr Phe Pro Gly Leu Leu

65 70 75 80

Ser Leu Gly Ile Asp Met Gly Leu Glu Phe Pro Val Arg Gln Ile Asp

85 90 95

Val Cys Gly Ile Leu His Arg Arg Glu Met Glu Leu Lys Arg Leu Ala

100 105 110

Val Asp Ser Ser Phe Gly Arg Lys Ala Tyr Met Ala Phe Ile Pro Glu

115 120 125

Gly Phe Gly Asn Met Leu Asp Trp Asp Gln Val Met Lys Phe Gln Arg

130 135 140

Lys Asn Gly Ser Leu Phe Ser Thr Pro Ser Thr Thr Ala Val Ala Leu

›SEQUENCE LISTING · 7 of 53

145 150 155 160

Ile His Lys Tyr Asn Asp Gln Ala Leu Gln Tyr Leu Asn Leu Leu Val

165 170 175

Asn Glu Phe Gly Ser Ala Val Pro Ala Met Tyr Pro Ser Arg Val His

180 185 190

Cys Gln Leu Ser Met Val Asp Ala Leu Glu Lys Met Gly Ile Ser Gln

195 200 205

Arg Phe Val Ser Glu Ile Glu Ser Ile Leu Asp Met Ala Tyr Asn Cys

210 215 220

Trp Leu Gln Asn Asp Glu Glu Leu Met Met Asp Ile Ala Thr Phe Ala

225 230 235 240

Met Ala Phe Arg Leu Leu Arg Met Asn Gly Tyr Asp Val Ser Ser Asp

245 250 255

Glu Leu Ser His Val Ala Gly Ala Ser Thr Phe His Asp Ser Leu Gln

260 265 270

Gly Tyr Leu Asn Asp Thr Lys Ser Leu Leu Glu Leu Tyr Lys Thr Ser

275 280 285

Lys Val Thr Leu Ser Glu Asn Asp Leu Ile Leu Asp Arg Ile Gly Ser

290 295 300

Trp Ser Gly Asn Leu Leu Lys Asp Lys Met Cys Cys Ser Lys Val Gln

305 310 315 320

Lys Thr Arg Phe Leu Glu Arg Cys Cys Lys Gln Asn Leu Asn Ser His

325 330 335

Phe His Leu Gly Gly Ser Val Tyr Val Cys Val Leu Xaa Phe Gln Ile

340 345 350

Glu Tyr Ala Val Asn Phe Pro Leu Tyr Ser Thr Leu Glu Arg Leu Glu

355 360 365

His Lys Arg Asn Ile Glu His Phe Asp Ala Trp Gly Ser Leu Met Leu

370 375 380

Thr Thr Lys Ser Ser Ser Phe Arg Ile Asn Gln Glu Phe Leu Ala Leu

385 390 395 400

Ala Val Glu Asp Phe Ser Phe Ser Gln Arg Val Tyr Arg Asp Glu Leu

405 410 415

Arg His Leu Asp Ser Trp Val Lys Glu Asn Lys Leu Asp Gln Leu Gln

420 425 430

Phe Ala Arg Gln Lys Leu Thr Tyr Cys Tyr Leu Ser Ala Ala Ala Thr

435 440 445

Val Phe Ser Ser Glu Leu Ser Asp Ala Arg Ile Ser Trp Ala Lys Asn

450 455 460

Gly Val Leu Thr Thr Val Val Asp Asp Phe Phe Asp Val Gly Gly Ser

465 470 475 480

Lys Glu Glu Leu Glu Asn Leu Ile Ala Leu Val Glu Lys Trp His Gly

485 490 495

His His Ala Val Glu Phe Tyr Ser Glu Gln Val Lys Ile Val Phe Ser

500 505 510

Ala Ile Tyr Thr Thr Val Asn His Leu Gly Ala Met Ala Ser Ala Ala

515 520 525

Gln Gly Arg Asp Leu Thr Asn His Leu Val Glu Ile Trp Leu Asp Leu

530 535 540

Leu Arg Ser Met Met Val Glu Ala Glu Trp Gln Arg Cys Gln Tyr Val

545 550 555 560

Pro Thr Val Glu Glu Tyr Met Thr Asn Ala Val Val Ser Phe Ala Leu

565 570 575

Gly Pro Ile Val Leu Pro Ala Leu Tyr Phe Val Gly Gln Glu Leu Leu

580 585 590

Glu His Ala Val Lys Asp Glu Glu Tyr Asp Lys Leu Phe Arg Leu Val

595 600 605

Ser Thr Cys Gly Arg Leu Leu Asn Asp Tyr Gln Ser Leu Glu Arg Glu

610 615 620

Gly Asn Gln Gly Lys Leu Asn Ser Val Ser Leu Leu Val Leu His Ser

625 630 635 640

Gly Gly Ser Met Ser Ile Glu Ala Ala Lys Lys Ala Met Gln Lys Ser

645 650 655

Ile Asp Val Ser Arg Arg Asp Leu Leu Arg Leu Val Leu Arg Lys Glu

660 665 670

Ser Ala Val Pro Arg Pro Cys Lys Glu Leu Phe Trp Lys Met Cys Lys

675 680 685

Ile Leu His Leu Phe Tyr Ser Gln Asn Asp Gly Phe Ser Ser Pro Lys

690 695 700

Glu Met Val Ser Ala Val Asn Ala Val Ile Asn Glu Pro Leu Lys Val

705 710 715 720

Gln Asn Ser Thr Thr Phe Leu Ser Ser Ser Ser Arg

725 730

<210> SEQ ID NO 25

<211> LENGTH: 468

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (435)

<221> NAME/KEY: unsure

<222> LOCATION: (448)

<400> SEQUENCE: 25

cttaagtttc ccttctacac cacactggac cgtctagatc acaagaggaa cattgaacgt 60

tttgatgcaa aggattccca gatgttaaag acggaatact tgcttcctca tgccaatcaa 120

gatattctag ctttggctgt tgaagatttc agtagttctc aatctatata ccaggatgaa 180

cttaattatc ttgagtgttg ggtgaaagat gaaaagctcg atcagctgcc atttgcacgc 240

cagaagttga catattgcta cctttctgct gctgctacca tattcccccg tgaattgtct 300

gaagcccgca ttgcatgggc taaaaatggt gtacctgaca actgttgttg atgacttctt 360

tgatcttggg ggatcaaaag aagaactaag aaaacctcat tgctttagtt gagaagtggg 420

atggacatca aagangagtt ctactcanaa caagtaagaa tagttttt 468

<210> SEQ ID NO 26

<211> LENGTH: 140

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (34)

<221> NAME/KEY: UNSURE

<222> LOCATION: (112)

<221> NAME/KEY: UNSURE

<222> LOCATION: (130)

<400> SEQUENCE: 26

Leu Lys Phe Pro Phe Tyr Thr Thr Leu Asp Arg Leu Asp His Lys Arg

1 5 10 15

Asn Ile Glu Arg Phe Asp Ala Lys Asp Ser Gln Met Leu Lys Thr Glu

20 25 30

Tyr Xaa Leu Pro His Ala Asn Gln Asp Ile Leu Ala Leu Ala Val Glu

35 40 45

Asp Phe Ser Ser Ser Gln Ser Ile Tyr Gln Asp Glu Leu Asn Tyr Leu

50 55 60

Glu Cys Trp Val Lys Asp Glu Lys Leu Asp Gln Leu Pro Phe Ala Arg

65 70 75 80

Gln Lys Leu Thr Tyr Cys Tyr Leu Ser Ala Ala Ala Thr Ile Phe Pro

85 90 95

Arg Glu Leu Ser Glu Ala Arg Ile Ala Trp Ala Lys Asn Gly Val Xaa

100 105 110

Thr Thr Val Val Asp Asp Phe Phe Asp Leu Gly Gly Ser Lys Glu Glu

115 120 125

Leu Xaa Asn Leu Ile Ala Leu Val Glu Lys Trp Asp

130 135 140

<210> SEQ ID NO 27

<211> LENGTH: 531

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 27

gctgctgcaa ccatatccgc tcatgaattc tctgatgctc gcattgcatg tgcgaaagct 60

attgcactcg tacttgttac tgatgacttc tttgatgttg gagcatccaa ggaagaacaa 120

gaaaacctca tagcattagt agagaagtgg gatcaccatc acaaagttga gttctactcc 180

gagcaagtag aagtagtatt ttctgctttt tataccacag ttaatcagct tggagaaatg 240

gcttccgcag tacaaaactg cgatgttaca aagcacttgg atgaaacatg gctacattac 300

atgaggtctg tggcaaccga ggcaaaatgg caacggaatc aatatgtgcc aacagttgag 360

gaatacatga cagacgcgct tacctcatac gggatgggcc cgattatgct cacatcactg 420

tattttgtcc aaaacaaact tatgaagcac attatcaaag accggagtac agtgagttgc 480

ttagactaat gggtacatgt ggccgtctct tgaatgatac caaggcttgg a 531

<210> SEQ ID NO 28

<211> LENGTH: 177

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (153)..(154)..(155)

<400> SEQUENCE: 28

Ala Ala Ala Thr Ile Ser Ala His Glu Phe Ser Asp Ala Arg Ile Ala

1 5 10 15

Cys Ala Lys Ala Ile Ala Leu Val Leu Val Thr Asp Asp Phe Phe Asp

20 25 30

Val Gly Ala Ser Lys Glu Glu Gln Glu Asn Leu Ile Ala Leu Val Glu

35 40 45

Lys Trp Asp His His His Lys Val Glu Phe Tyr Ser Glu Gln Val Glu

50 55 60

Val Val Phe Ser Ala Phe Tyr Thr Thr Val Asn Gln Leu Gly Glu Met

65 70 75 80

Ala Ser Ala Val Gln Asn Cys Asp Val Thr Lys His Leu Asp Glu Thr

85 90 95

Trp Leu His Tyr Met Arg Ser Val Ala Thr Glu Ala Lys Trp Gln Arg

100 105 110

Asn Gln Tyr Val Pro Thr Val Glu Glu Tyr Met Thr Asp Ala Leu Thr

115 120 125

Ser Tyr Gly Met Gly Pro Ile Met Leu Thr Ser Leu Tyr Phe Val Gln

130 135 140

Asn Lys Leu Met Lys His Ile Ile Xaa Xaa Xaa Glu Tyr Ser Glu Leu

145 150 155 160

Leu Arg Leu Met Gly Thr Cys Gly Arg Leu Leu Asn Asp Thr Lys Ala

165 170 175

Trp

<210> SEQ ID NO 29

›SEQUENCE LISTING · 8 of 53

<211> LENGTH: 476

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 29

cttacagaca ttcagtagaa cagaagacat aacatttgca accgaggaat gcctccttct 60

tactatagca taaagcgatg ggaatctatc tttagaggta ctgtttgatt gaggttgtat 120

acaactaatt aacactacga caaacgtacg tatttataga gaccaaaaac tatgtgtaaa 180

attaagtaat ttaatgtctg tcgattgatt gtattgatca atgtcgacac gtagattggt 240

tacgccctag acttcccctg gaaagcaagt ctgccacgtg ttgaaacaag aatgtatctg 300

gagcaatatg gcgccggcag agatgtgtgg atcggtaagt cgctttacag gtaaaagcac 360

ggtaattaac caacaattgt gaaatactct ctcctttatc atgtcatcat cctcaaatat 420

actgggcgta cacggtccta aacctttctg gaaggctggc actccgtgaa caaagg 476

<210> SEQ ID NO 30

<211> LENGTH: 48

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (9)

<400> SEQUENCE: 30

Ile Val Leu Ile Asn Val Asp Thr Xaa Ile Gly Tyr Ala Leu Asp Phe

1 5 10 15

Pro Trp Lys Ala Ser Leu Pro Arg Val Glu Thr Arg Met Tyr Leu Glu

20 25 30

Gln Tyr Gly Ala Gly Arg Asp Val Trp Ile Gly Lys Ser Leu Tyr Arg

35 40 45

<210> SEQ ID NO 31

<211> LENGTH: 518

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (245)

<221> NAME/KEY: unsure

<222> LOCATION: (406)

<221> NAME/KEY: unsure

<222> LOCATION: (427)

<221> NAME/KEY: unsure

<222> LOCATION: (461)

<221> NAME/KEY: unsure

<222> LOCATION: (466)

<221> NAME/KEY: unsure

<222> LOCATION: (492)

<221> NAME/KEY: unsure

<222> LOCATION: (512)

<400> SEQUENCE: 31

gccaccttcc ctctttctct tcttctaacc atttcccttt ttcatcttct tcttctatct 60

ctcttcacca cttctccaaa tcctctctgg gtgccgtgtt gtccgagact aacgacaaac 120

aagagataag atgcagagca atatccaaac cacgaacaca agaatattca gacatatttc 180

agggtagtct agcaacgtta aagtttcgtg agattaatgt ggaagatgac atagaagagg 240

agcangacat cgggaaggtt tttgtggcaa atgagatcaa gaagagagtg gacactatca 300

aatcaatatt gggttccatg gaagatggag agataacagt atctgcttat gacactgctt 360

gggttgctct gatagaagat gttcaaggga agtggtgctc ctcaanttca accgagtcta 420

gaatggntaa gcgaaaaaat caacaccctg gatgggtcaa nggggngata agggaattat 480

cccaagccca angttcgggt tttttaaaac gntgggtt 518

<210> SEQ ID NO 32

<211> LENGTH: 127

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (81)

<400> SEQUENCE: 32

His Leu Pro Ser Phe Ser Ser Ser Asn His Phe Pro Phe Ser Ser Ser

1 5 10 15

Ser Ser Ile Ser Leu His His Phe Ser Lys Ser Ser Leu Gly Ala Val

20 25 30

Leu Ser Glu Thr Asn Asp Lys Gln Glu Ile Arg Cys Arg Ala Ile Ser

35 40 45

Lys Pro Arg Thr Gln Glu Tyr Ser Asp Ile Phe Gln Gly Ser Leu Ala

50 55 60

Thr Leu Lys Phe Arg Glu Ile Asn Val Glu Asp Asp Ile Glu Glu Glu

65 70 75 80

Xaa Asp Ile Gly Lys Val Phe Val Ala Asn Glu Ile Lys Lys Arg Val

85 90 95

Asp Thr Ile Lys Ser Ile Leu Gly Ser Met Glu Asp Gly Glu Ile Thr

100 105 110

Val Ser Ala Tyr Asp Thr Ala Trp Val Ala Leu Ile Glu Asp Val

115 120 125

<210> SEQ ID NO 33

<211> LENGTH: 1157

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (40)

<221> NAME/KEY: unsure

<222> LOCATION: (44)

<221> NAME/KEY: unsure

<222> LOCATION: (60)

<221> NAME/KEY: unsure

<222> LOCATION: (64)

<221> NAME/KEY: unsure

<222> LOCATION: (712)

<221> NAME/KEY: unsure

<222> LOCATION: (721)

<221> NAME/KEY: unsure

<222> LOCATION: (723)

<221> NAME/KEY: unsure

<222> LOCATION: (732)

<221> NAME/KEY: unsure

<222> LOCATION: (738)

<221> NAME/KEY: unsure

<222> LOCATION: (757)

<221> NAME/KEY: unsure

<222> LOCATION: (763)

<221> NAME/KEY: unsure

<222> LOCATION: (782)

<221> NAME/KEY: unsure

<222> LOCATION: (809)

<221> NAME/KEY: unsure

<222> LOCATION: (813)

<221> NAME/KEY: unsure

<222> LOCATION: (824)

<221> NAME/KEY: unsure

<222> LOCATION: (826)..(827)

<221> NAME/KEY: unsure

<222> LOCATION: (849)..(850)

<221> NAME/KEY: unsure

<222> LOCATION: (858)

<221> NAME/KEY: unsure

<222> LOCATION: (863)

<221> NAME/KEY: unsure

<222> LOCATION: (872)

<221> NAME/KEY: unsure

<222> LOCATION: (874)

<221> NAME/KEY: unsure

<222> LOCATION: (888)

<221> NAME/KEY: unsure

<222> LOCATION: (930)..(931)

<221> NAME/KEY: unsure

<222> LOCATION: (938)

<221> NAME/KEY: unsure

<222> LOCATION: (942)

<221> NAME/KEY: unsure

<222> LOCATION: (961)

<221> NAME/KEY: unsure

<222> LOCATION: (967)

<221> NAME/KEY: unsure

<222> LOCATION: (971)

<221> NAME/KEY: unsure

<222> LOCATION: (981)

<221> NAME/KEY: unsure

<222> LOCATION: (988)..(989)

<221> NAME/KEY: unsure

<222> LOCATION: (993)

<221> NAME/KEY: unsure

<222> LOCATION: (999)

<221> NAME/KEY: unsure

<222> LOCATION: (1024)

<221> NAME/KEY: unsure

<222> LOCATION: (1028)

<221> NAME/KEY: unsure

<222> LOCATION: (1031)

<221> NAME/KEY: unsure

<222> LOCATION: (1035)

<221> NAME/KEY: unsure

<222> LOCATION: (1040)

<221> NAME/KEY: unsure

<222> LOCATION: (1049)..(1050)

<221> NAME/KEY: unsure

<222> LOCATION: (1068)

<221> NAME/KEY: unsure

<222> LOCATION: (1089)

<221> NAME/KEY: unsure

<222> LOCATION: (1105)..(1106)

<221> NAME/KEY: unsure

<222> LOCATION: (1121)

<221> NAME/KEY: unsure

<222> LOCATION: (1124)

<221> NAME/KEY: unsure

<222> LOCATION: (1131)

<221> NAME/KEY: unsure

<222> LOCATION: (1136)..(1137)

<221> NAME/KEY: unsure

<222> LOCATION: (1155)

<400> SEQUENCE: 33

atgcaaaacc aacaagataa tggatcttgg ggtgtcagcn gggnctgact catcggtcan 60

caangatgtt ctcctatcca cgttggcatg tgttcttgcg ttgaagagat ggaatgttgg 120

cagagagaac atttggagag gactgcattt catcgggagg aatttctctg ttgctatgga 180

cgagcagttc acttctccta taggtttcaa cttcaccttt cctggtttgc ttagcctcgg 240

cattgatatg ggtttagaat ttcctgtaag acaaattgat gtctgtggca ttcttcaccg 300

ccgggagatg gaattgaaaa ggctggctgt ggatagttct tttggaagaa aagcatatat 360

ggcttttatc ccagaaggat tcggaaatat gctggactgg gatcaagtta tgaagtttca 420

gaggaagaat ggatcattgt tcagcactcc ttccacaact gctgttgcat taatccacaa 480

atacaacgac caagcccttc aatacctaaa tttgcttgtc aatgaatttg gcagtgcagt 540

accagcaatg tatccttcaa gggtacattg tcagctttca atggtggacg cgcttgaaaa 600

aatgggaatt tctcagcgct ttgtcagtga aataaaaaac atcctggaca tggcatacaa 660

ttgctggtta cagaaagatg aggaaatcat gatggacata gcaacatttg cnatggcatt 720

ncnccttttg angatgantg gttacaatgt ttcctcngat ganctgtctc acgttgctgg 780

ancttccact ttccatgaat cactacaang atntttaaat gatncnnaat ccctactgga 840

attgtacann acctcaanag tcnccttatc ananaacgat ctgatctnag atcgcatagg 900

ttcctggtct ggcgacttat tgaacgatan natgtgcngt tntaaggtgc aaaaagactc 960

nattttngga nagatggact ntgctgtnna ttntccctnt gtattccccc ctgggaccgt 1020

›SEQUENCE LISTING · 9 of 53

ctanaacncc naganaaacn tccaacatnn tgatgcttgg ggttctcnga tgctcacaaa 1080

ccaaatccnc atcttttcgt atccnncccc acaaatccct nccnttggcc nctccnnaaa 1140

ttcccccttt ccccnca 1157

<210> SEQ ID NO 34

<211> LENGTH: 259

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (217)..(218)

<221> NAME/KEY: UNSURE

<222> LOCATION: (221)

<221> NAME/KEY: UNSURE

<222> LOCATION: (223)

<221> NAME/KEY: UNSURE

<222> LOCATION: (231)

<221> NAME/KEY: UNSURE

<222> LOCATION: (238)

<221> NAME/KEY: UNSURE

<222> LOCATION: (247)..(248)

<221> NAME/KEY: UNSURE

<222> LOCATION: (252)..(253)

<400> SEQUENCE: 34

Leu Leu Ser Thr Leu Ala Cys Val Leu Ala Leu Lys Arg Trp Asn Val

1 5 10 15

Gly Arg Glu Asn Ile Trp Arg Gly Leu His Phe Ile Gly Arg Asn Phe

20 25 30

Ser Val Ala Met Asp Glu Gln Phe Thr Ser Pro Ile Gly Phe Asn Phe

35 40 45

Thr Phe Pro Gly Leu Leu Ser Leu Gly Ile Asp Met Gly Leu Glu Phe

50 55 60

Pro Val Arg Gln Ile Asp Val Cys Gly Ile Leu His Arg Arg Glu Met

65 70 75 80

Glu Leu Lys Arg Leu Ala Val Asp Ser Ser Phe Gly Arg Lys Ala Tyr

85 90 95

Met Ala Phe Ile Pro Glu Gly Phe Gly Asn Met Leu Asp Trp Asp Gln

100 105 110

Val Met Lys Phe Gln Arg Lys Asn Gly Ser Leu Phe Ser Thr Pro Ser

115 120 125

Thr Thr Ala Val Ala Leu Ile His Lys Tyr Asn Asp Gln Ala Leu Gln

130 135 140

Tyr Leu Asn Leu Leu Val Asn Glu Phe Gly Ser Ala Val Pro Ala Met

145 150 155 160

Tyr Pro Ser Arg Val His Cys Gln Leu Ser Met Val Asp Ala Leu Glu

165 170 175

Lys Met Gly Ile Ser Gln Arg Phe Val Ser Glu Ile Lys Asn Ile Leu

180 185 190

Asp Met Ala Tyr Asn Cys Trp Leu Gln Lys Asp Glu Glu Ile Met Met

195 200 205

Asp Ile Ala Thr Phe Ala Met Ala Xaa Xaa Leu Leu Xaa Met Xaa Gly

210 215 220

Tyr Asn Val Ser Ser Asp Xaa Leu Ser His Val Ala Gly Xaa Ser Thr

225 230 235 240

Phe His Glu Ser Leu Gln Xaa Xaa Leu Asn Asp Xaa Xaa Ser Leu Leu

245 250 255

Glu Leu Tyr

<210> SEQ ID NO 35

<211> LENGTH: 1436

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 35

ccacgcgtcc gagcagttgc agttgcagcg gcctcctcct ctgcttcctc cctcctcctc 60

ctcaccatgg tgctggctgc gcacgatccc cctccccttg tgttcgacgc tgcccgcctg 120

agcggcctct ccgacatccc gcagcagttc atctggccgg cggacgagag ccccaccccg 180

gactccgccg aggagctggc cgtgccgctc atcgacctct ccggggacgc cgccgaggtg 240

gtccggcagg tccggcgcgc ctgcgacctg cacggcttct tccaggtggt ggggcacggc 300

atcgacgcgg cgctgacggc ggaggcccac cgctgcatgg acgccttctt cacgctgccg 360

ctcccggaca agcagcgcgc gcagcgccgc cagggggaca gctgcggcta cgccagcagc 420

ttcacgggcc ggttcgcgtc caagctgccc tggaaggaga cgctgtcgtt ccgctacacc 480

gacgacgacg acggcgacaa gtccaaggac gtcgtggcgt cctacttcgt ggacaagctg 540

ggcgaggggt accggcacca cggggaggtg tacgggcgct actgctctga gatgagccgt 600

ctgtcgctgg agctcatgga ggtgctaggc gagagcctgg gcgtgggccg gcgccacttc 660

cggcgcttct tccaggggaa cgactccatc atgcgcctca actactaccc gccgtgccag 720

cggccctacg acacgctggg cacggggccg cattgcgacc ccacgtcgct caccatcctg 780

caccaggacg acgtgggcgg actccaggtg ttcgacgccg ccacgctcgc gtggcgctcc 840

atcaggcccc gcccgggcgc cttcgtcgtc aacatcggcg acaccttcat ggcgctctcc 900

aacgggcgct acaggagctg cctccaccgc gccgtcgtca acagccgggt ggcacgccgc 960

tcgctcgcct tcttcctgtg cccggagatg gacaaggtgg tcaggccgcc caaggagctg 1020

gtggacgacg ccaacccgag ggcgtacccg gacttcacgt ggaggacgct gctggacttc 1080

accatgaggc actacaggtc ggacatgagg acgctcgagg ccttctccaa ctggctcagc 1140

accagtagca atggcggaca gcacctgctg gagaagaagt aggcatgcta tttgggtatg 1200

gaagatggtg gatgtaagca aacaaagcca aattaagcag agtaggttaa ttaaggttgg 1260

ctgatgatcc atttagggaa ggagctgatc tccctgactc cctcctccaa ttttctcaac 1320

caaatttata tagtataata ataataataa aatagcaagt aatagttgta tcgtattatt 1380

attaattaat ttattagctg gtaggcaagt agtattaaaa aaaaaaaaaa aaaaag 1436

<210> SEQ ID NO 36

<211> LENGTH: 393

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 36

Pro Arg Val Arg Ala Val Ala Val Ala Ala Ala Ser Ser Ser Ala Ser

1 5 10 15

Ser Leu Leu Leu Leu Thr Met Val Leu Ala Ala His Asp Pro Pro Pro

20 25 30

Leu Val Phe Asp Ala Ala Arg Leu Ser Gly Leu Ser Asp Ile Pro Gln

35 40 45

Gln Phe Ile Trp Pro Ala Asp Glu Ser Pro Thr Pro Asp Ser Ala Glu

50 55 60

Glu Leu Ala Val Pro Leu Ile Asp Leu Ser Gly Asp Ala Ala Glu Val

65 70 75 80

Val Arg Gln Val Arg Arg Ala Cys Asp Leu His Gly Phe Phe Gln Val

85 90 95

Val Gly His Gly Ile Asp Ala Ala Leu Thr Ala Glu Ala His Arg Cys

100 105 110

Met Asp Ala Phe Phe Thr Leu Pro Leu Pro Asp Lys Gln Arg Ala Gln

115 120 125

Arg Arg Gln Gly Asp Ser Cys Gly Tyr Ala Ser Ser Phe Thr Gly Arg

130 135 140

Phe Ala Ser Lys Leu Pro Trp Lys Glu Thr Leu Ser Phe Arg Tyr Thr

145 150 155 160

Asp Asp Asp Asp Gly Asp Lys Ser Lys Asp Val Val Ala Ser Tyr Phe

165 170 175

Val Asp Lys Leu Gly Glu Gly Tyr Arg His His Gly Glu Val Tyr Gly

180 185 190

Arg Tyr Cys Ser Glu Met Ser Arg Leu Ser Leu Glu Leu Met Glu Val

195 200 205

Leu Gly Glu Ser Leu Gly Val Gly Arg Arg His Phe Arg Arg Phe Phe

210 215 220

Gln Gly Asn Asp Ser Ile Met Arg Leu Asn Tyr Tyr Pro Pro Cys Gln

225 230 235 240

Arg Pro Tyr Asp Thr Leu Gly Thr Gly Pro His Cys Asp Pro Thr Ser

245 250 255

Leu Thr Ile Leu His Gln Asp Asp Val Gly Gly Leu Gln Val Phe Asp

260 265 270

Ala Ala Thr Leu Ala Trp Arg Ser Ile Arg Pro Arg Pro Gly Ala Phe

275 280 285

Val Val Asn Ile Gly Asp Thr Phe Met Ala Leu Ser Asn Gly Arg Tyr

290 295 300

Arg Ser Cys Leu His Arg Ala Val Val Asn Ser Arg Val Ala Arg Arg

305 310 315 320

Ser Leu Ala Phe Phe Leu Cys Pro Glu Met Asp Lys Val Val Arg Pro

325 330 335

Pro Lys Glu Leu Val Asp Asp Ala Asn Pro Arg Ala Tyr Pro Asp Phe

340 345 350

Thr Trp Arg Thr Leu Leu Asp Phe Thr Met Arg His Tyr Arg Ser Asp

355 360 365

Met Arg Thr Leu Glu Ala Phe Ser Asn Trp Leu Ser Thr Ser Ser Asn

370 375 380

Gly Gly Gln His Leu Leu Glu Lys Lys

385 390

<210> SEQ ID NO 37

<211> LENGTH: 1361

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 37

gcacgagggc agccgtggtg ttcgacgcgg cgatcctgag caagcaggag gcgatcccgg 60

cgcagttcgt ctggccggcc gacgaggcgc cggcggcgga cgacggcgtg gtggaggaga 120

tcgccatccc cgtcgtcgac ctggccgcgt tcctcgccag cggcggcatt gggcgcgacg 180

tggcggaggc gtgcgagcgg cacgggttct tccaggtggt gaaccacggc gtcgacccgg 240

cgctgctcgc cgaggcgtac cgctgctgtg acgcgttcta cgcgcggccg ctcgccgaga 300

agcagcgcgc gcggcgccgc cccggcgaga accacggcta cgcgagcagc ttcacggggc 360

ggttcgactg caagctgccg tggaaggaga ccatgtcgtt caactgctcc gccgcgccgg 420

›SEQUENCE LISTING · 10 of 53

ggaacgcgcg catggtcgcc gactatttcg tcgacgccct cggcgaggaa taccgccaca 480

tgggggaggt gtaccaggag tactgcgacg tgatgacgcg gctggcgctg gacgtgacgg 540

aggtgctcgc ggtggcgctc gggctcggcc gcggcgagct ccgcggcttc ttcgccgacg 600

gcgacccggt gatgcggctg aaccactacc cgccgtgccg gcagccgcac ctgacgctgg 660

gcacgggccc gcaccgcgac ccgacgtcgc tgacgctgct ccaccaggac gacgtcggcg 720

gcctgcaggt gctccccgac gacgccgcgg cggcggcggg cgggtggcgc gccgtgcgcc 780

cgcgcgccga cgcgttcgtc gtcaacatcg gcgacacgtt cgcggcgctc accaacgggc 840

gccacgccag ctgcctccac cgcgccgtgg tgaacggccg cgtcgcgcgc aggtcgctca 900

ccttcttcct caacccgcgc ctcgaccgcg tcgtgtcgcc gccgccggcg ctcgtcgacg 960

ccgcgcaccc gcgcgcgttc ccggacttca cgtggcgcga gttcctcgag ttcacgcaga 1020

ggcactaccg gtcggacacc aacaccatgg acgccttcgt cgcctggatc aagcaaagga 1080

atggctatga aagcctcgac aagtactaga cagatgggat gagaagatca ggtcatcaag 1140

attcaagagt tgatggacga ataaaccaga gtttctactc gatcgatcgc cattttcgtg 1200

tttatttgtt tttaagatat atatagcgtg cattgcaaca tgcatgtttt aaataaggga 1260

acatgtcagt gaatttgaaa aaggtttaaa ttgtatatat attgcaatct ctttcgtatt 1320

gttcgagaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a 1361

<210> SEQ ID NO 38

<211> LENGTH: 368

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 38

Thr Arg Ala Ala Val Val Phe Asp Ala Ala Ile Leu Ser Lys Gln Glu

1 5 10 15

Ala Ile Pro Ala Gln Phe Val Trp Pro Ala Asp Glu Ala Pro Ala Ala

20 25 30

Asp Asp Gly Val Val Glu Glu Ile Ala Ile Pro Val Val Asp Leu Ala

35 40 45

Ala Phe Leu Ala Ser Gly Gly Ile Gly Arg Asp Val Ala Glu Ala Cys

50 55 60

Glu Arg His Gly Phe Phe Gln Val Val Asn His Gly Val Asp Pro Ala

65 70 75 80

Leu Leu Ala Glu Ala Tyr Arg Cys Cys Asp Ala Phe Tyr Ala Arg Pro

85 90 95

Leu Ala Glu Lys Gln Arg Ala Arg Arg Arg Pro Gly Glu Asn His Gly

100 105 110

Tyr Ala Ser Ser Phe Thr Gly Arg Phe Asp Cys Lys Leu Pro Trp Lys

115 120 125

Glu Thr Met Ser Phe Asn Cys Ser Ala Ala Pro Gly Asn Ala Arg Met

130 135 140

Val Ala Asp Tyr Phe Val Asp Ala Leu Gly Glu Glu Tyr Arg His Met

145 150 155 160

Gly Glu Val Tyr Gln Glu Tyr Cys Asp Val Met Thr Arg Leu Ala Leu

165 170 175

Asp Val Thr Glu Val Leu Ala Val Ala Leu Gly Leu Gly Arg Gly Glu

180 185 190

Leu Arg Gly Phe Phe Ala Asp Gly Asp Pro Val Met Arg Leu Asn His

195 200 205

Tyr Pro Pro Cys Arg Gln Pro His Leu Thr Leu Gly Thr Gly Pro His

210 215 220

Arg Asp Pro Thr Ser Leu Thr Leu Leu His Gln Asp Asp Val Gly Gly

225 230 235 240

Leu Gln Val Leu Pro Asp Asp Ala Ala Ala Ala Ala Gly Gly Trp Arg

245 250 255

Ala Val Arg Pro Arg Ala Asp Ala Phe Val Val Asn Ile Gly Asp Thr

260 265 270

Phe Ala Ala Leu Thr Asn Gly Arg His Ala Ser Cys Leu His Arg Ala

275 280 285

Val Val Asn Gly Arg Val Ala Arg Arg Ser Leu Thr Phe Phe Leu Asn

290 295 300

Pro Arg Leu Asp Arg Val Val Ser Pro Pro Pro Ala Leu Val Asp Ala

305 310 315 320

Ala His Pro Arg Ala Phe Pro Asp Phe Thr Trp Arg Glu Phe Leu Glu

325 330 335

Phe Thr Gln Arg His Tyr Arg Ser Asp Thr Asn Thr Met Asp Ala Phe

340 345 350

Val Ala Trp Ile Lys Gln Arg Asn Gly Tyr Glu Ser Leu Asp Lys Tyr

355 360 365

<210> SEQ ID NO 39

<211> LENGTH: 1270

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 39

gcacgagata gcaagctcat agctcaagct cataagctca tagatgattt cttctgcatg 60

caactctcac agaagcagaa ggctcagaga aagattggag aacattgtgg ctatgctaat 120

agcttcattg gaagattctc ctccaaactt ccttggaagg aaacactttc tttccattat 180

tctgcagata aatccagaaa aactgtggag gattatttcc ttaatgtcat gggggaagat 240

ttcaagcaat ttgggagtgt gttccaagaa tactgtgaag ccatgagcaa actctctctt 300

gggataatgg agcttctggg gatgagccta ggagttggca gggaatgttt cagagatttc 360

ttcgaaggaa atgagtcggt tatgaggttg aattactacc caccatgcca aaaacctgag 420

ttagctttag gaactggacc tcattgtgac cctacatccc taaccattct ccaccaagat 480

caagtcgagg gcctccaagt ctttgttgat ggaagatggt actctgtcgc tcctaaagaa 540

gatgctttcg ttgtcaatat tggcgacaca tttatggctc tatcgaatgg gatgttcaag 600

agttgcttgc atagagcagt tgtaaacaac aaaattgtga gaaaatcact tgctttcttc 660

ctatgtccaa atagagacaa agtggtcacc cctccaaaag atctaatcag ctacgaaaat 720

tcaagaacat acccagattt cacatggcca agccttcttg aatttacaca gaaacactac 780

agatctgaca caaaaacact tgatgctttc tcgagatggc tactagaaaa aaacaatccg 840

aacaggggtg cccccacaaa tgttccatgg ccaacaaaat ctataaactg agctttaact 900

tgcatctgct tgaatgagga agatatgaca tcatataaag tgaaaaagag ggtgcttttg 960

gtgggtcgac ttgtgaagaa aaagataaca gaaacatcaa aaagggaagg gctaatcatc 1020

agtaattagt tgtggtgtta gtgttgggag aatcctatat ggtgcaaatg gaagaagaac 1080

tgaggccata ttctttggaa taaagagagt tgatttgtgt agtgaagtta ttcgatgttg 1140

tcactactta tgcattatta gttgtcttga actatgttgt agttatgtga tatgcatgta 1200

tgtagaactt ttaattaatt aataataaac tgcattgcat tttctgtcat caaaaaaaaa 1260

aaaaaaaaaa 1270

<210> SEQ ID NO 40

<211> LENGTH: 296

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 40

Ala Arg Asp Ser Lys Leu Ile Ala Gln Ala His Lys Leu Ile Asp Asp

1 5 10 15

Phe Phe Cys Met Gln Leu Ser Gln Lys Gln Lys Ala Gln Arg Lys Ile

20 25 30

Gly Glu His Cys Gly Tyr Ala Asn Ser Phe Ile Gly Arg Phe Ser Ser

35 40 45

Lys Leu Pro Trp Lys Glu Thr Leu Ser Phe His Tyr Ser Ala Asp Lys

50 55 60

Ser Arg Lys Thr Val Glu Asp Tyr Phe Leu Asn Val Met Gly Glu Asp

65 70 75 80

Phe Lys Gln Phe Gly Ser Val Phe Gln Glu Tyr Cys Glu Ala Met Ser

85 90 95

Lys Leu Ser Leu Gly Ile Met Glu Leu Leu Gly Met Ser Leu Gly Val

100 105 110

Gly Arg Glu Cys Phe Arg Asp Phe Phe Glu Gly Asn Glu Ser Val Met

115 120 125

Arg Leu Asn Tyr Tyr Pro Pro Cys Gln Lys Pro Glu Leu Ala Leu Gly

130 135 140

Thr Gly Pro His Cys Asp Pro Thr Ser Leu Thr Ile Leu His Gln Asp

145 150 155 160

Gln Val Glu Gly Leu Gln Val Phe Val Asp Gly Arg Trp Tyr Ser Val

165 170 175

Ala Pro Lys Glu Asp Ala Phe Val Val Asn Ile Gly Asp Thr Phe Met

180 185 190

Ala Leu Ser Asn Gly Met Phe Lys Ser Cys Leu His Arg Ala Val Val

195 200 205

Asn Asn Lys Ile Val Arg Lys Ser Leu Ala Phe Phe Leu Cys Pro Asn

210 215 220

Arg Asp Lys Val Val Thr Pro Pro Lys Asp Leu Ile Ser Tyr Glu Asn

225 230 235 240

Ser Arg Thr Tyr Pro Asp Phe Thr Trp Pro Ser Leu Leu Glu Phe Thr

245 250 255

Gln Lys His Tyr Arg Ser Asp Thr Lys Thr Leu Asp Ala Phe Ser Arg

260 265 270

Trp Leu Leu Glu Lys Asn Asn Pro Asn Arg Gly Ala Pro Thr Asn Val

275 280 285

Pro Trp Pro Thr Lys Ser Ile Asn

290 295

<210> SEQ ID NO 41

<211> LENGTH: 634

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 41

›SEQUENCE LISTING · 11 of 53

gcacgagcct gaccatcctg taccaggacg acgtcggggg gctcgacgtc cggcggcgct 60

ccgacggcga gtgggtccgc gtcaggcccg tgcccgactc gttcatcatc aacgtcggcg 120

acctcatcca ggtgtggagc aacgacaggt acgagagcgc ggagcaccgg gtgtcggtga 180

actcggcgag ggagaggttc tccatgccct acttcttcaa cccggcgacc tacaccatgg 240

tggagccggt ggaggagctg gtgagcaagg acgatccgcc caggtacgac gcctacaact 300

ggggcgactt cttcagcacc aggaagaaca gcaacttcaa gaagctcaac gtggagaaca 360

ttcagatcgc gcatttcaag aagagcctcg tcctcgccta actactgcta ctgctaggat 420

ccatgccatt gccatgtcgt cttcagattc agagcacgcc atgtcgtcgc tagcttcgtg 480

gtagaacaaa taatgatgtg cgtgctgtgt gtaagcatgg atatggatgt gaatatgtaa 540

tatgatgagc actcctactt tggtatgttt gggaataaca gacttgtgtt ggtctggttc 600

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 634

<210> SEQ ID NO 42

<211> LENGTH: 132

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 42

Thr Ser Leu Thr Ile Leu Tyr Gln Asp Asp Val Gly Gly Leu Asp Val

1 5 10 15

Arg Arg Arg Ser Asp Gly Glu Trp Val Arg Val Arg Pro Val Pro Asp

20 25 30

Ser Phe Ile Ile Asn Val Gly Asp Leu Ile Gln Val Trp Ser Asn Asp

35 40 45

Arg Tyr Glu Ser Ala Glu His Arg Val Ser Val Asn Ser Ala Arg Glu

50 55 60

Arg Phe Ser Met Pro Tyr Phe Phe Asn Pro Ala Thr Tyr Thr Met Val

65 70 75 80

Glu Pro Val Glu Glu Leu Val Ser Lys Asp Asp Pro Pro Arg Tyr Asp

85 90 95

Ala Tyr Asn Trp Gly Asp Phe Phe Ser Thr Arg Lys Asn Ser Asn Phe

100 105 110

Lys Lys Leu Asn Val Glu Asn Ile Gln Ile Ala His Phe Lys Lys Ser

115 120 125

Leu Val Leu Ala

130

<210> SEQ ID NO 43

<211> LENGTH: 1066

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 43

gcacgagctc cacctcagtc acattattcc cttagacttt tcttcagccc ttagcttacc 60

tgattctcac gcatggcctc actgtcaacc caatgatgat gcatcatcat catcatcatc 120

atcatccata cccatcatag acctcatgga tcccaatgct atggacctca taggccatgc 180

atgtgagaag tggggtgctt tccaattgaa gaaccatggc ataccttttg gtgtcattga 240

agatgttgaa gaagaggcta aaagactttt tgctcttccc actgaacaga agttgaaggc 300

tcttcgatct cccggtggtg ccactgggta tggcagagca aggatttcac cgttcttccc 360

aaagtttatg tggcacgaag gattcaccat cattggctct ccctctcacg atgccaaaaa 420

gatatggcct aatgattatg cacgcttttg tgatttgatg gagaattatg agaagcaaat 480

gaaggttcta gcggatagac taacagagat gatattcaac ttaatggaca taagtgagga 540

aaaaagaaag tgggttggtg caagcaacat aagtgaagct gttcagttaa atttctaccc 600

aagttgtccc gagccaaacc gagccatggg cttagcccct cacaccgaca cttctctctt 660

cacaattctt caccaaagtc aaatcactgg tcttcaaatc ttcaaggaag gaaaagggtg 720

ggttcctgtg caccctcacc ctaataccct ggtcgtgcac acaggcgatc tcttgcacat 780

catttccaac gcaaggtttc gctgtgcact tcaccgcgta acggtgaata ggacatggga 840

acgttactct gtggcttatt tctattctcc accgatggat tatgtagttt ctcctttggt 900

tcactctgtt gctcgttttc gtgatgtgac tgtgaaggag tatattggga ttaaggctaa 960

gaattttgga gaagcattgt cctttattag tacttgaaac tatgtatgta tcttatgatt 1020

ttgttatatg tattaattcc ttaaataaaa aaaaaaaaaa aaaaaa 1066

<210> SEQ ID NO 44

<211> LENGTH: 331

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 44

His Glu Leu His Leu Ser His Ile Ile Pro Leu Asp Phe Ser Ser Ala

1 5 10 15

Leu Ser Leu Pro Asp Ser His Ala Trp Pro His Cys Gln Pro Asn Asp

20 25 30

Asp Ala Ser Ser Ser Ser Ser Ser Ser Ser Ile Pro Ile Ile Asp Leu

35 40 45

Met Asp Pro Asn Ala Met Asp Leu Ile Gly His Ala Cys Glu Lys Trp

50 55 60

Gly Ala Phe Gln Leu Lys Asn His Gly Ile Pro Phe Gly Val Ile Glu

65 70 75 80

Asp Val Glu Glu Glu Ala Lys Arg Leu Phe Ala Leu Pro Thr Glu Gln

85 90 95

Lys Leu Lys Ala Leu Arg Ser Pro Gly Gly Ala Thr Gly Tyr Gly Arg

100 105 110

Ala Arg Ile Ser Pro Phe Phe Pro Lys Phe Met Trp His Glu Gly Phe

115 120 125

Thr Ile Ile Gly Ser Pro Ser His Asp Ala Lys Lys Ile Trp Pro Asn

130 135 140

Asp Tyr Ala Arg Phe Cys Asp Leu Met Glu Asn Tyr Glu Lys Gln Met

145 150 155 160

Lys Val Leu Ala Asp Arg Leu Thr Glu Met Ile Phe Asn Leu Met Asp

165 170 175

Ile Ser Glu Glu Lys Arg Lys Trp Val Gly Ala Ser Asn Ile Ser Glu

180 185 190

Ala Val Gln Leu Asn Phe Tyr Pro Ser Cys Pro Glu Pro Asn Arg Ala

195 200 205

Met Gly Leu Ala Pro His Thr Asp Thr Ser Leu Phe Thr Ile Leu His

210 215 220

Gln Ser Gln Ile Thr Gly Leu Gln Ile Phe Lys Glu Gly Lys Gly Trp

225 230 235 240

Val Pro Val His Pro His Pro Asn Thr Leu Val Val His Thr Gly Asp

245 250 255

Leu Leu His Ile Ile Ser Asn Ala Arg Phe Arg Cys Ala Leu His Arg

260 265 270

Val Thr Val Asn Arg Thr Trp Glu Arg Tyr Ser Val Ala Tyr Phe Tyr

275 280 285

Ser Pro Pro Met Asp Tyr Val Val Ser Pro Leu Val His Ser Val Ala

290 295 300

Arg Phe Arg Asp Val Thr Val Lys Glu Tyr Ile Gly Ile Lys Ala Lys

305 310 315 320

Asn Phe Gly Glu Ala Leu Ser Phe Ile Ser Thr

325 330

<210> SEQ ID NO 45

<211> LENGTH: 790

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 45

gcacgagggg aaccccgcct tctgcgacac gatcgcgtcc gcggccaaga acgtgtggga 60

gctgcagcgc accgtggaga cgatgatcct ggagggcctg ggcgtcggcg aggagcacat 120

cggcgcgcat ctggacacgc tcgcccacag cgtccggctg tcgcgctacg ggcccccgcc 180

ggacgcggag accagcatgt ccctgcaggc gcaccgcgac gacagcatca tgacggcgat 240

cgtgcagcac ggcgtggagg gcctcgaggt gcaggctcag gacgggatct ggctcacggt 300

gccgcctgag ccggacttgt tcaccttcgt cgccggcgag ctgttcacgg tcgtgactaa 360

cgggagggtg ccgggttgct tccaccgcgt gaggacgccg agcaaccgcg agcgcctgtc 420

ggcgctgttc ggctgcccgg ggaaggacgg cgtccttctg ggcgccattg acgagctcgt 480

cgacgacgaa caccctctgc tgtaccgtcc ctgcaccaac gacggctaca caacgttccg 540

gcactcggac gaagggcgca aggccagtga tccattgaag gccttctgtg gggtggagaa 600

agatgatcgc cgttcgtaag tcaagctcac ttcacctcac cattctgaag tcgtcaagct 660

ccgttctcca caccattctg aagccgacat tgctaggtta cccatgtaat gattgcgacg 720

ccattctgaa gtcctattgc tactgaactt tactcaaaaa aaaaaaaaaa aaaaaaaaaa 780

aaaaaaaaaa 790

<210> SEQ ID NO 46

<211> LENGTH: 205

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 46

His Glu Gly Asn Pro Ala Phe Cys Asp Thr Ile Ala Ser Ala Ala Lys

1 5 10 15

Asn Val Trp Glu Leu Gln Arg Thr Val Glu Thr Met Ile Leu Glu Gly

20 25 30

Leu Gly Val Gly Glu Glu His Ile Gly Ala His Leu Asp Thr Leu Ala

35 40 45

His Ser Val Arg Leu Ser Arg Tyr Gly Pro Pro Pro Asp Ala Glu Thr

50 55 60

Ser Met Ser Leu Gln Ala His Arg Asp Asp Ser Ile Met Thr Ala Ile

65 70 75 80

Val Gln His Gly Val Glu Gly Leu Glu Val Gln Ala Gln Asp Gly Ile

85 90 95

Trp Leu Thr Val Pro Pro Glu Pro Asp Leu Phe Thr Phe Val Ala Gly

100 105 110

Glu Leu Phe Thr Val Val Thr Asn Gly Arg Val Pro Gly Cys Phe His

›SEQUENCE LISTING · 12 of 53

115 120 125

Arg Val Arg Thr Pro Ser Asn Arg Glu Arg Leu Ser Ala Leu Phe Gly

130 135 140

Cys Pro Gly Lys Asp Gly Val Leu Leu Gly Ala Ile Asp Glu Leu Val

145 150 155 160

Asp Asp Glu His Pro Leu Leu Tyr Arg Pro Cys Thr Asn Asp Gly Tyr

165 170 175

Thr Thr Phe Arg His Ser Asp Glu Gly Arg Lys Ala Ser Asp Pro Leu

180 185 190

Lys Ala Phe Cys Gly Val Glu Lys Asp Asp Arg Arg Ser

195 200 205

<210> SEQ ID NO 47

<211> LENGTH: 445

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (445)

<400> SEQUENCE: 47

ctcgtgccga attcggcacg aggccctcac cattctcctc atggaccccc acgtctccgg 60

cctgcaggtc ctcaaggacg gcgcccagtg gatcgccgtt gacccccggc ccaacgccct 120

cgtcgtcaac ctaggcgacc agttgcaggc gctgagcaac ggcgcgtaca agagcgtctg 180

gcaccgcgcg gtggtcaatg cggcgcggga gcgcctgtcg gtggcatctt tcctgtgccc 240

gtgcaacagc gcggtgatcg gcccggtggc caagctcgtc ggcgacgggg acgagcccgt 300

gtaccgcagc tacacctacg acgagtacta caagaagttc tggagcagga acctggacca 360

aggaccattg cctggagctc ttcacgggtc agaaagtgat ccgtgtgcac aagcttgcct 420

catccacgac gcaacattac tcaan 445

<210> SEQ ID NO 48

<211> LENGTH: 110

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 48

Leu Thr Ile Leu Leu Met Asp Pro His Val Ser Gly Leu Gln Val Leu

1 5 10 15

Lys Asp Gly Ala Gln Trp Ile Ala Val Asp Pro Arg Pro Asn Ala Leu

20 25 30

Val Val Asn Leu Gly Asp Gln Leu Gln Ala Leu Ser Asn Gly Ala Tyr

35 40 45

Lys Ser Val Trp His Arg Ala Val Val Asn Ala Ala Arg Glu Arg Leu

50 55 60

Ser Val Ala Ser Phe Leu Cys Pro Cys Asn Ser Ala Val Ile Gly Pro

65 70 75 80

Val Ala Lys Leu Val Gly Asp Gly Asp Glu Pro Val Tyr Arg Ser Tyr

85 90 95

Thr Tyr Asp Glu Tyr Tyr Lys Lys Phe Trp Ser Arg Asn Leu

100 105 110

<210> SEQ ID NO 49

<211> LENGTH: 1431

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (1309)

<221> NAME/KEY: unsure

<222> LOCATION: (1339)

<221> NAME/KEY: unsure

<222> LOCATION: (1351)

<221> NAME/KEY: unsure

<222> LOCATION: (1402)

<221> NAME/KEY: unsure

<222> LOCATION: (1429)

<400> SEQUENCE: 49

gagcagtttc agttgcagcg gcctcctcct ctgcttcctc cctcctcctc ctcaccatgg 60

tgctggctgc gcacgatccc cctccccttg tgttcgacgc tgcccgcctg agcggcctct 120

ccgacatccc gcagcagttc atctggccgg cggacgagag ccccaccccg gactccgccg 180

aggagctggc cgtgccgctc atcgacctct ccggggaagc cgccgaggtg gtccggcagg 240

tccggcgcgc ctgcgacctg cacggcttct tccaggtggt ggggcacggc atcgacgcgg 300

cgctgacggc ggaggcccac cgctgcatgg acgccttctt cacgctgccg ctcccggaca 360

agcagcgcgc gcagcgccgt cagggggaca gctgcggcta cgccagcagc ttcacgggcc 420

ggttcgcgtc caagctgccc tggaaggaga cgctgtcgtt ccggtacacc gacgacgacg 480

acggcgacaa gtccaaggac gtcgtggcgt cctacttcgt ggacaagctg ggcgaggggt 540

accggcacca cggggaggtg tacgggcgct actgctctga gatgagccgt ctgtcgctgg 600

agctcatgga ggtgctaggc gagagcctgg gcgtgggccg gcgccacttc cggcgcttct 660

tccaggggaa cgactccatc atgcgcctca actactaccc gccgtgccag cggccctacg 720

acacgctggg cacggggccg cattgcgacc ccacgtcgct caccatcctg caccaggacg 780

acgtgggcgg actccaggtg ttcgacgccg ccacgctcgc gtggcgctcc atcaggcccc 840

gcccgggcgc cttcgtcgtc aacatcggcg acaccttcat ggcgctctcc aacgggcgct 900

acaggagctg cctccaccgc gccgtcgtca acagccgggt ggcacgccgc tcgctcgcct 960

tcttcctgtg cccggagatg gacaaggtgg tcaggccgcc caaggagctg gtggacgacg 1020

ccaacccgag ggcgtacccg gacttcacgt ggaggacgct gctggacttc accatgaggc 1080

actacaggtc ggacatgagg acgctcgagg ccttctccaa ctggctcagc accagtagca 1140

atggcggaca gcacctgctg gagaagaagt aggatgctat ttgggtatgg aagatggtgg 1200

atgtaagcaa acaaagccaa attaagcaga gtaggttaat taagggttgg ctgatgatca 1260

attaagggaa aggagctgat ttccctgact ccctcctcca aattttcanc aaattaatat 1320

ataatatata taaatacant aatagttgta nctattataa taataattta ctgagcagta 1380

tattaataca tttttacaag gntattcatt tggttaaaaa aaaaaaaanc a 1431

<210> SEQ ID NO 50

<211> LENGTH: 371

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 50

Met Val Leu Ala Ala His Asp Pro Pro Pro Leu Val Phe Asp Ala Ala

1 5 10 15

Arg Leu Ser Gly Leu Ser Asp Ile Pro Gln Gln Phe Ile Trp Pro Ala

20 25 30

Asp Glu Ser Pro Thr Pro Asp Ser Ala Glu Glu Leu Ala Val Pro Leu

35 40 45

Ile Asp Leu Ser Gly Glu Ala Ala Glu Val Val Arg Gln Val Arg Arg

50 55 60

Ala Cys Asp Leu His Gly Phe Phe Gln Val Val Gly His Gly Ile Asp

65 70 75 80

Ala Ala Leu Thr Ala Glu Ala His Arg Cys Met Asp Ala Phe Phe Thr

85 90 95

Leu Pro Leu Pro Asp Lys Gln Arg Ala Gln Arg Arg Gln Gly Asp Ser

100 105 110

Cys Gly Tyr Ala Ser Ser Phe Thr Gly Arg Phe Ala Ser Lys Leu Pro

115 120 125

Trp Lys Glu Thr Leu Ser Phe Arg Tyr Thr Asp Asp Asp Asp Gly Asp

130 135 140

Lys Ser Lys Asp Val Val Ala Ser Tyr Phe Val Asp Lys Leu Gly Glu

145 150 155 160

Gly Tyr Arg His His Gly Glu Val Tyr Gly Arg Tyr Cys Ser Glu Met

165 170 175

Ser Arg Leu Ser Leu Glu Leu Met Glu Val Leu Gly Glu Ser Leu Gly

180 185 190

Val Gly Arg Arg His Phe Arg Arg Phe Phe Gln Gly Asn Asp Ser Ile

195 200 205

Met Arg Leu Asn Tyr Tyr Pro Pro Cys Gln Arg Pro Tyr Asp Thr Leu

210 215 220

Gly Thr Gly Pro His Cys Asp Pro Thr Ser Leu Thr Ile Leu His Gln

225 230 235 240

Asp Asp Val Gly Gly Leu Gln Val Phe Asp Ala Ala Thr Leu Ala Trp

245 250 255

Arg Ser Ile Arg Pro Arg Pro Gly Ala Phe Val Val Asn Ile Gly Asp

260 265 270

Thr Phe Met Ala Leu Ser Asn Gly Arg Tyr Arg Ser Cys Leu His Arg

275 280 285

Ala Val Val Asn Ser Arg Val Ala Arg Arg Ser Leu Ala Phe Phe Leu

290 295 300

Cys Pro Glu Met Asp Lys Val Val Arg Pro Pro Lys Glu Leu Val Asp

305 310 315 320

Asp Ala Asn Pro Arg Ala Tyr Pro Asp Phe Thr Trp Arg Thr Leu Leu

325 330 335

Asp Phe Thr Met Arg His Tyr Arg Ser Asp Met Arg Thr Leu Glu Ala

340 345 350

Phe Ser Asn Trp Leu Ser Thr Ser Ser Asn Gly Gly Gln His Leu Leu

355 360 365

Glu Lys Lys

370

<210> SEQ ID NO 51

<211> LENGTH: 432

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (33)

<221> NAME/KEY: unsure

<222> LOCATION: (342)

<400> SEQUENCE: 51

ggcagccgtg gtgttcgacg cggcgatcct gancaagcag gaggcgatcc cggcgcagtt 60

cgtctggccg gccgacgagg cgccggcggc ggacgacggc gtggtggagg agatcgccat 120

ccccgtcgtc gacctggccg cgttcctcgc cagcggcggc attgggcgcg acgtggcgga 180

ggcgtgcgag cggcacgggt tcttccaggt ggtgaaccac ggcgtcgacc cggcgctgct 240

cgccgaggcg taccgctgct gtgacgcgtt ctacgcgcgg ccgctcgccg agaagcagcg 300

cgcgcggcgc cgccccggcg agaaccacgg ctacgcgagc ancttcacgg ggcggttccg 360

›SEQUENCE LISTING · 13 of 53

actgcaagct ggcccgtgga aagagaccat gtcgttcaac tggctccgcc cgcgcccggg 420

ggaaccccgc aa 432

<210> SEQ ID NO 52

<211> LENGTH: 132

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (11)

<221> NAME/KEY: UNSURE

<222> LOCATION: (114)

<400> SEQUENCE: 52

Ala Ala Val Val Phe Asp Ala Ala Ile Leu Xaa Lys Gln Glu Ala Ile

1 5 10 15

Pro Ala Gln Phe Val Trp Pro Ala Asp Glu Ala Pro Ala Ala Asp Asp

20 25 30

Gly Val Val Glu Glu Ile Ala Ile Pro Val Val Asp Leu Ala Ala Phe

35 40 45

Leu Ala Ser Gly Gly Ile Gly Arg Asp Val Ala Glu Ala Cys Glu Arg

50 55 60

His Gly Phe Phe Gln Val Val Asn His Gly Val Asp Pro Ala Leu Leu

65 70 75 80

Ala Glu Ala Tyr Arg Cys Cys Asp Ala Phe Tyr Ala Arg Pro Leu Ala

85 90 95

Glu Lys Gln Arg Ala Arg Arg Arg Pro Gly Glu Asn His Gly Tyr Ala

100 105 110

Ser Xaa Phe Thr Gly Arg Phe Arg Leu Gln Ala Gly Pro Trp Lys Glu

115 120 125

Thr Met Ser Phe

130

<210> SEQ ID NO 53

<211> LENGTH: 522

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (30)

<221> NAME/KEY: unsure

<222> LOCATION: (341)

<221> NAME/KEY: unsure

<222> LOCATION: (445)

<221> NAME/KEY: unsure

<222> LOCATION: (450)

<221> NAME/KEY: unsure

<222> LOCATION: (484)

<221> NAME/KEY: unsure

<222> LOCATION: (521)

<400> SEQUENCE: 53

gagatagcaa gctcatagct caagctcatn aagctcatag atgatttctt ctgcatgcaa 60

ctctcacaga agcagaaggc tcagagaaag attggagaac attgtggcta tgctaatagc 120

ttcattggaa gattctcctc caaacttcct tggaaggaaa cacttctttc cattattctg 180

cagataaatc cagaaaaact gtggaggatt atttccttaa tgtcatgggg gaagatttca 240

agcaatttgg gagtgtgttc caagaatact gtgaagccat gagcaaactc tctcttggga 300

taatggagct tctggggatg agcctaggag ttggcaggga ngtttcagag atttcttcga 360

aggaaatgag tcggttatga ggttgaatta ctacccacca tgccaaaaac ctgagttagc 420

tttaggaact ggacctcatt gtgancctan atccctaacc attctccacc aagatcaagt 480

cgangggctc caagtctttg ttgaagggaa gatggtaccc nt 522

<210> SEQ ID NO 54

<211> LENGTH: 169

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (148)

<221> NAME/KEY: UNSURE

<222> LOCATION: (150)

<221> NAME/KEY: UNSURE

<222> LOCATION: (161)

<400> SEQUENCE: 54

Asp Ser Lys Leu Ile Ala Gln Ala His Lys Leu Ile Asp Asp Phe Phe

1 5 10 15

Cys Met Gln Leu Ser Gln Lys Gln Lys Ala Gln Arg Lys Ile Gly Glu

20 25 30

His Cys Gly Tyr Ala Asn Ser Phe Ile Gly Arg Phe Ser Ser Lys Leu

35 40 45

Pro Trp Lys Glu Thr Leu Ser Phe His Tyr Ser Ala Asp Lys Ser Arg

50 55 60

Lys Thr Val Glu Asp Tyr Phe Leu Asn Val Met Gly Glu Asp Phe Lys

65 70 75 80

Gln Phe Gly Ser Val Phe Gln Glu Tyr Cys Glu Ala Met Ser Lys Leu

85 90 95

Ser Leu Gly Ile Met Glu Leu Leu Gly Met Ser Leu Gly Val Gly Gln

100 105 110

Glu Cys Phe Arg Asp Phe Phe Glu Gly Asn Glu Ser Val Met Arg Leu

115 120 125

Asn Tyr Tyr Pro Pro Cys Gln Lys Pro Glu Leu Ala Leu Gly Thr Gly

130 135 140

Pro His Cys Xaa Pro Xaa Ser Leu Thr Ile Leu His Gln Asp Gln Val

145 150 155 160

Xaa Gly Leu Gln Val Phe Val Glu Gly

165

<210> SEQ ID NO 55

<211> LENGTH: 490

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (394)

<221> NAME/KEY: unsure

<222> LOCATION: (399)

<221> NAME/KEY: unsure

<222> LOCATION: (427)

<221> NAME/KEY: unsure

<222> LOCATION: (432)

<221> NAME/KEY: unsure

<222> LOCATION: (445)

<221> NAME/KEY: unsure

<222> LOCATION: (448)..(449)

<221> NAME/KEY: unsure

<222> LOCATION: (455)

<221> NAME/KEY: unsure

<222> LOCATION: (460)

<221> NAME/KEY: unsure

<222> LOCATION: (475)

<400> SEQUENCE: 55

cctgaccatc ctgtaccagg acgacgtcgg ggggctcgac gtccggcggc gctccgacgg 60

cgagtgggtc cgcgtcaggc ccgtgcccga ctcgttcatc atcaacgtcg gcgacctcat 120

ccaggtgtgg agcaacgaca ggtacgagag cgcggacacc gggtgtcggt gaactcggcg 180

agggagaggt tctccatgcc ctacttcttc aacccggcga cctacaccat ggtggagccg 240

gtggaggagc tggtgagcaa ggacgatccg cccaggtacg acgcctacaa ctggggcgac 300

ttcttcagca ccaggaagaa cagcaacttc aagaagtcaa cgtggagaca ttcagatcgc 360

gcatttcaag aagagctcgt ctcgctaact actntactnt agatccatgc attgcatgcg 420

gctcaantag anacgcatgt gtccnacnng tgganaaaan atatggcgtc cttgnacatg 480

aatgattaaa 490

<210> SEQ ID NO 56

<211> LENGTH: 50

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 56

Leu Thr Ile Leu Tyr Gln Asp Asp Val Gly Gly Leu Asp Val Arg Arg

1 5 10 15

Arg Ser Asp Gly Glu Trp Val Arg Val Arg Pro Val Pro Asp Ser Phe

20 25 30

Ile Ile Asn Val Gly Asp Leu Ile Gln Val Trp Ser Asn Asp Arg Tyr

35 40 45

Glu Ser

50

<210> SEQ ID NO 57

<211> LENGTH: 536

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (291)

<221> NAME/KEY: unsure

<222> LOCATION: (394)

<221> NAME/KEY: unsure

<222> LOCATION: (401)

<221> NAME/KEY: unsure

<222> LOCATION: (406)

<221> NAME/KEY: unsure

<222> LOCATION: (436)

<221> NAME/KEY: unsure

<222> LOCATION: (439)

<221> NAME/KEY: unsure

<222> LOCATION: (457)

<221> NAME/KEY: unsure

<222> LOCATION: (482)

<221> NAME/KEY: unsure

<222> LOCATION: (493)

<221> NAME/KEY: unsure

<222> LOCATION: (508)

<221> NAME/KEY: unsure

<222> LOCATION: (522)

<400> SEQUENCE: 57

aattaaaaat gaacactact ctctctgaag cctacaaagc acaccctctc cacctccgtg 60

acattattcc cttagacttt tcttcagccc atagcttacc tgattctcac gcatggtctc 120

actctcaacc caacgatgat gattatgtct cattcaatga tgatgcatca tcatcatcat 180

tcatacccat catagacctc atggatccaa atgccatgga acaaataggc catgcatgtg 240

agaaatgggg tgctttccaa ttgaagaacc atgggcatac ccttttgtgt nattgaagat 300

gtagaagaag aggctaaaag gctctttgct cttcccactg aacagaagtt gaagggctct 360

tcgatctcct ggtggtgcga ggggtatggc agancaagga nttcancgtt cttcccaaag 420

ttcagtgggc aagaanggnt taccatcatt gggtctncct ctcatgatgg ccaaaaagat 480

antggcctaa cgnatcatgc aagggttngg gaattggatg gnagattaag gaaaaa 536

<210> SEQ ID NO 58

<211> LENGTH: 148

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (90)

<221> NAME/KEY: UNSURE

<222> LOCATION: (129)

<221> NAME/KEY: UNSURE

<222> LOCATION: (131)

<221> NAME/KEY: UNSURE

<222> LOCATION: (133)

<221> NAME/KEY: UNSURE

<222> LOCATION: (143)..(144)

<400> SEQUENCE: 58

Met Asn Thr Thr Leu Ser Glu Ala Tyr Lys Ala His Pro Leu His Leu

1 5 10 15

Arg Asp Ile Ile Pro Leu Asp Phe Ser Ser Ala His Ser Leu Pro Asp

20 25 30

Ser His Ala Trp Ser His Ser Gln Pro Asn Asp Asp Asp Tyr Val Ser

35 40 45

Phe Asn Asp Asp Ala Ser Ser Ser Ser Phe Ile Pro Ile Ile Asp Leu

›SEQUENCE LISTING · 14 of 53

50 55 60

Met Asp Pro Asn Ala Met Glu Gln Ile Gly His Ala Cys Glu Lys Trp

65 70 75 80

Gly Ala Phe Gln Leu Lys Asn His Gly Xaa Pro Phe Cys Val Ile Glu

85 90 95

Asp Val Glu Glu Glu Ala Lys Arg Leu Phe Ala Leu Pro Thr Glu Gln

100 105 110

Lys Leu Lys Gly Ser Ser Ile Ser Trp Trp Cys Glu Gly Tyr Gly Arg

115 120 125

Xaa Arg Xaa Ser Xaa Phe Phe Pro Lys Phe Ser Gly Gln Glu Xaa Xaa

130 135 140

Thr Ile Ile Gly

145

<210> SEQ ID NO 59

<211> LENGTH: 459

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (378)

<221> NAME/KEY: unsure

<222> LOCATION: (422)

<400> SEQUENCE: 59

gggaaccccg ccttctgcga cacgatcgcg tccgcggcca agaacgtgtg ggagctgcag 60

cgcaccgtgg agacgatgat cctggagggc ctgggcgtcg gcgaggagca catcggcgcg 120

catctggaca cgctcgccca cagcgtccgg ctgtcgcgct acgggccccc gccggacgcg 180

gagaccagca tgtccctgca ggcgcaccgc gacgacagca tcatgacggc gatcgtgcag 240

cacggcgtgg agggcctcga ggtgcaggct caggacggga tctggctcac ggtgccgcct 300

gagccggact tgttcacctt cgtcgccggc gagctgttca cggtcgtgac taacgggagg 360

gtgccgggtt gcttcaancg cgtgaggacc cgagcaaccg cgagcgcctg tcggcgcttt 420

cngctgcccg gggaaggacg gcgtccttct gggcgcaat 459

<210> SEQ ID NO 60

<211> LENGTH: 129

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (122)

<400> SEQUENCE: 60

Phe Cys Asp Thr Ile Ala Ser Ala Ala Lys Asn Val Trp Glu Leu Gln

1 5 10 15

Arg Thr Val Glu Thr Met Ile Leu Glu Gly Leu Gly Val Gly Glu Glu

20 25 30

His Ile Gly Ala His Leu Asp Thr Leu Ala His Ser Val Arg Leu Ser

35 40 45

Arg Tyr Gly Pro Pro Pro Asp Ala Glu Thr Ser Met Ser Leu Gln Ala

50 55 60

His Arg Asp Asp Ser Ile Met Thr Ala Ile Val Gln His Gly Val Glu

65 70 75 80

Gly Leu Glu Val Gln Ala Gln Asp Gly Ile Trp Leu Thr Val Pro Pro

85 90 95

Glu Pro Asp Leu Phe Thr Phe Val Ala Gly Glu Leu Phe Thr Val Val

100 105 110

Thr Asn Gly Arg Val Pro Gly Cys Phe Xaa Arg Val Arg Thr Arg Ala

115 120 125

Thr

<210> SEQ ID NO 61

<211> LENGTH: 1209

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 61

gcacgaggaa acagtgtttg ctggtaaaaa gaaagaaaaa agaagctgaa agtgttttca 60

ggcagatgcc tacatttttc tctctatcct agttgtaaca tatgattgaa agtgatgtac 120

ttaatatggt tgcaagaatc atattaatac ctatggctgg ttatgtacac gcctcttcgg 180

tgttcttatg tgctgttgct ggtgatcctt gagatattga ttgccatgct agcaaatttt 240

atctgttgca ggtataggag gaaaagagac aggctaattg aacgcgcttc tgttgcacgc 300

ttgcctttga aatggggcaa tgtccatgcc tattgctaca aatctgttat tgatggcatt 360

gagcatattg ccatggtgaa aggtgatatt ggtgacggcc aagacatttt ggtgagggtg 420

cactctgagt gcctcacagg cgacattttt ggatccgcaa gatgtgattg tggcgaccag 480

cttgcgatgg caatggagat gattgagaag actaatacac cccttccagc cttctccgtg 540

gacatgaagg aaggggtatt ggtctgggcc acaagcttcg tgcttataac ttacaggatg 600

atgggcgtga tactgtggaa gctaacgagg aacttggtct tcctgtggac tcacgggaat 660

atgggattgg cgcacagata ttgcgggatc taggagtccg ttcaatgaag ctgatgacta 720

ataaccctgc gaaatatagt ggtctcaagg gctatggatt gagcattgcg ggcagggtgc 780

ccttgattac gccaattacc agtgagaacc gtaggtacct ggagactaaa agaacaaaga 840

tgggacatgt atatgggttg gccaatgcgc aggccaacca agcaaatggc agccagagca 900

cagaagagaa tcattaggcc cccaagcatg tttccattat tattccagca atccagcttg 960

gtgtggtggc acaaaagagt gatatgattc ttcgtttggg gagttcctga aatcttctcc 1020

cccgatattt ccgtagattc tttcgcaaaa aagccgatat ttcggtagat cgatttgttc 1080

ttcaattgta actgtaggtt tcaaggtggc taatgaacgg taggtttcaa gatggcccgt 1140

aatgatatac acggagtttg tcgtcaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1200

aaaaaaaaa 1209

<210> SEQ ID NO 62

<211> LENGTH: 250

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (123)

<400> SEQUENCE: 62

Met Tyr Thr Pro Leu Arg Cys Ser Tyr Val Leu Leu Leu Val Ile Leu

1 5 10 15

Glu Ile Leu Ile Ala Met Leu Ala Asn Phe Ile Cys Cys Arg Tyr Arg

20 25 30

Arg Lys Arg Asp Arg Leu Ile Glu Arg Ala Ser Val Ala Arg Leu Pro

35 40 45

Leu Lys Trp Gly Asn Val His Ala Tyr Cys Tyr Lys Ser Val Ile Asp

50 55 60

Gly Ile Glu His Ile Ala Met Val Lys Gly Asp Ile Gly Asp Gly Gln

65 70 75 80

Asp Ile Leu Val Arg Val His Ser Glu Cys Leu Thr Gly Asp Ile Phe

85 90 95

Gly Ser Ala Arg Cys Asp Cys Gly Asp Gln Leu Ala Met Ala Met Glu

100 105 110

Met Ile Glu Lys Thr Asn Thr Pro Leu Pro Xaa Leu Arg Gly His Glu

115 120 125

Gly Arg Gly Ile Gly Leu Gly His Lys Leu Arg Ala Tyr Asn Leu Gln

130 135 140

Asp Asp Gly Arg Asp Thr Val Glu Ala Asn Glu Glu Leu Gly Leu Pro

145 150 155 160

Val Asp Ser Arg Glu Tyr Gly Ile Gly Ala Gln Ile Leu Arg Asp Leu

165 170 175

Gly Val Arg Ser Met Lys Leu Met Thr Asn Asn Pro Ala Lys Tyr Ser

180 185 190

Gly Leu Lys Gly Tyr Gly Leu Ser Ile Ala Gly Arg Val Pro Leu Ile

195 200 205

Thr Pro Ile Thr Ser Glu Asn Arg Arg Tyr Leu Glu Thr Lys Arg Thr

210 215 220

Lys Met Gly His Val Tyr Gly Leu Ala Asn Ala Gln Ala Asn Gln Ala

225 230 235 240

Asn Gly Ser Gln Ser Thr Glu Glu Asn His

245 250

<210> SEQ ID NO 63

<211> LENGTH: 1454

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 63

gcacgaggat cttagtacga gtgcattcgg agtgcctaac tggggacata tttggatcag 60

cgagatgcga ttatgggaat taacttgctc tggcaatgac catgattgag aaaactggcc 120

ggggtgtagt agtctatctt cgtggccatg agggtagggg tattgggctg ggtcacaagc 180

ttcaagcata caacttacaa gatgatgggc gcgatactgt cgaggcaaac ttggagctag 240

ggctgcctgc tgactcgcgg gagtacggta taggtgcaca gatattacgc catcttggtg 300

ccaggaccat gcggctgatg actaacaacc ctgccaagta cactggactc aagggctatg 360

gtttaagtgt ccttggtaga gtgccattgc taacaccgat aaccaccgag aataggcgct 420

atatggaaac aaagcgattg aagatggggc atgtttatgg aagccgctcc agtaatcatg 480

caaacggcag tggcttggct ggtgctggcg agagagagga gcaggatcag aatgacagtg 540

ctggcgtgca agagcgaact ccagaagctt gagtgcctaa ctctgcgttc cttagagctg 600

ctccaatggg agtcttaaaa agagatgcat cttaaaatat agggctccat ggcaaaaaaa 660

tcactccaac ggggacctca ttttacaaaa actcaggaaa caaatttggg ctccggtctc 720

attggtccca aatgtaggac ttgaaactat cggccctatt ctcgttccca cgcccccacg 780

accttcggtc ttttcctgtt gcattcgttc tcaagccgtc tctcccacaa accaaaccct 840

ctgcctgtcg agaagcattg gcgagatgga tggacggcca ccgccttttc ctcgttcacg 900

gccaacaggt tggcctcgtt gaacttccat ggagatggca ctagatgtga tagggagatc 960

atcagctgtg tcttctccgc cagatttgcg caggcctcct gtggccgtgg ctgcagagcg 1020

aaggcctgca agtatgtctg caacttccca acagacacaa ggacaagcgt cttggcatcg 1080

aatcgctgca gaccatgccg atcggagttt atcagtggca ccgatgatgt atcaagatcc 1140

›SEQUENCE LISTING · 15 of 53

attgtcaatg attgatgact ataggtaata acctttctgt tgagattagt agtcgattat 1200

atttgctcat gaattcaatc gatggtgatc tgggatctcc atggttgaga aattgaagtc 1260

aaggcctggt gaggttactt gtactggtag gtgctgatag atgtagtgct caccgatgct 1320

tgtgaattga attattggtt ctgaaaatat cctcacttca accatgcata cgagattatg 1380

agtaaatgtg aattccataa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1440

aaaaaaaaaa aaaa 1454

<210> SEQ ID NO 64

<211> LENGTH: 189

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (27)

<400> SEQUENCE: 64

Thr Arg Ile Leu Val Arg Val His Ser Glu Cys Leu Thr Gly Asp Ile

1 5 10 15

Phe Gly Ser Ala Arg Cys Asp Tyr Gly Asn Xaa Leu Ala Leu Ala Met

20 25 30

Thr Met Ile Glu Lys Thr Gly Arg Gly Val Val Val Tyr Leu Arg Gly

35 40 45

His Glu Gly Arg Gly Ile Gly Leu Gly His Lys Leu Gln Ala Tyr Asn

50 55 60

Leu Gln Asp Asp Gly Arg Asp Thr Val Glu Ala Asn Leu Glu Leu Gly

65 70 75 80

Leu Pro Ala Asp Ser Arg Glu Tyr Gly Ile Gly Ala Gln Ile Leu Arg

85 90 95

His Leu Gly Ala Arg Thr Met Arg Leu Met Thr Asn Asn Pro Ala Lys

100 105 110

Tyr Thr Gly Leu Lys Gly Tyr Gly Leu Ser Val Leu Gly Arg Val Pro

115 120 125

Leu Leu Thr Pro Ile Thr Thr Glu Asn Arg Arg Tyr Met Glu Thr Lys

130 135 140

Arg Leu Lys Met Gly His Val Tyr Gly Ser Arg Ser Ser Asn His Ala

145 150 155 160

Asn Gly Ser Gly Leu Ala Gly Ala Gly Glu Arg Glu Glu Gln Asp Gln

165 170 175

Asn Asp Ser Ala Gly Val Gln Glu Arg Thr Pro Glu Ala

180 185

<210> SEQ ID NO 65

<211> LENGTH: 1788

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 65

ccacgcgtcc gccaaatgga ccgtgttctt ctgagctccc acttgccatc tcacgctcat 60

gcaaacacga ggttgcagca gggtcctggt ggcctcaaca gtataggatt tgcagtcata 120

aaaaagggat gcttgaagtt aagatgttac gcgatcggcg atgaactcgg tggtccaggg 180

cgctttagta agcatttcat cgagaacagt aatggacctg ttttccaggg actcgatgcg 240

tctgctggtt cttttagaac tgtcggtgct gaaatcactc aggacactgg agatttcttt 300

gtcagtgacg cagagggtga tccagacaaa cctaccgatg ggttctcctc gatcgaccag 360

gctataggtg ctttgcgtga agggaagttt gttattgctg tagatgatga gaacggcgac 420

aacgaaggcg accttgtgat ggcggccact ctagtggacc agaagtcagt cgcattcatg 480

atcaggaatg gctctgggat tatctcggta ggcatggagg aggaggacct gactagactg 540

atgattccga tgatgtcccc tatcacagaa atcgaagata tcacggctgc tgcttccacg 600

gtgacagtgg atgccagggt tggcatatct acgggtgtgt cggctgcaga cagggcgaag 660

accatcctca ctctagcctc ctctgactcc aagcccagtg atctgaggag accaggccac 720

atattccccc tcaagtaccg gaacgggggc gtgctgaaga gagctggcca cacagaagcg 780

tcggtggatc tggttgcgtt ggcaggtctc cgtcctgtgt ccgttctctc caccgtcatg 840

gaccccaggg acggttcgat ggcaggcgta acggttctcc agcagatggc tatggagcat 900

gacataccca tcgtttcgat tgctgatctc atccggtata ggagaaagcg ggagaagctg 960

gtggagctga ttgctgtgtc tcgcttgccg acgaaatggg gcctcttccg agcctactgc 1020

taccagtcca ggctcgatgg aaccgagcac atcgctgttg tgaagggcga tatcggcgac 1080

ggcgaagagg tcctggtgag ggtgcactcg gagtgcctga cgggggacat cctcggctca 1140

gcccgctgcg actgcggcaa ccagctggag ctggcgatgc agctgatcga gaaggccggc 1200

cgtggcgtgc tcgtgtacct ccgcggccac gagggccgcg gcattggcct gggccagaag 1260

ctccgcgcct acaacctcca ggacgagggc cacgacaccg tcgaggccaa cgtcgagctc 1320

ggcctcgccg tggacgcccg cgagtacggc atcggcgccc agatactccg tgacatcggc 1380

gtgcgcacga tgcggctgat gaccaacaac cccgccaagt tcatcgggct caagggctac 1440

gggctcgccg tggtggggag ggtgccggtg atctctccca tcaccaagga gaaccagaag 1500

tacctcgaga ccaagcgcac caagatgggc cacgtctacg gctccgatct cccgggaagc 1560

ctgccggagt tcgccaaccc acaagaggcc cccacagaac aagacgacga cgataaccaa 1620

aactgataaa aaaactatac ggtgtcgttt ttttaggaaa agataccaat ataaccacaa 1680

attcttgtat tccactgaga acgagtattg cagagtattt atttatcaac tgcaaaatta 1740

ttaagtataa tagagataca aacttttatc aaaaaaaaaa aaaaaaag 1788

<210> SEQ ID NO 66

<211> LENGTH: 541

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 66

Pro Arg Val Arg Gln Met Asp Arg Val Leu Leu Ser Ser His Leu Pro

1 5 10 15

Ser His Ala His Ala Asn Thr Arg Leu Gln Gln Gly Pro Gly Gly Leu

20 25 30

Asn Ser Ile Gly Phe Ala Val Ile Lys Lys Gly Cys Leu Lys Leu Arg

35 40 45

Cys Tyr Ala Ile Gly Asp Glu Leu Gly Gly Pro Gly Arg Phe Ser Lys

50 55 60

His Phe Ile Glu Asn Ser Asn Gly Pro Val Phe Gln Gly Leu Asp Ala

65 70 75 80

Ser Ala Gly Ser Phe Arg Thr Val Gly Ala Glu Ile Thr Gln Asp Thr

85 90 95

Gly Asp Phe Phe Val Ser Asp Ala Glu Gly Asp Pro Asp Lys Pro Thr

100 105 110

Asp Gly Phe Ser Ser Ile Asp Gln Ala Ile Gly Ala Leu Arg Glu Gly

115 120 125

Lys Phe Val Ile Ala Val Asp Asp Glu Asn Gly Asp Asn Glu Gly Asp

130 135 140

Leu Val Met Ala Ala Thr Leu Val Asp Gln Lys Ser Val Ala Phe Met

145 150 155 160

Ile Arg Asn Gly Ser Gly Ile Ile Ser Val Gly Met Glu Glu Glu Asp

165 170 175

Leu Thr Arg Leu Met Ile Pro Met Met Ser Pro Ile Thr Glu Ile Glu

180 185 190

Asp Ile Thr Ala Ala Ala Ser Thr Val Thr Val Asp Ala Arg Val Gly

195 200 205

Ile Ser Thr Gly Val Ser Ala Ala Asp Arg Ala Lys Thr Ile Leu Thr

210 215 220

Leu Ala Ser Ser Asp Ser Lys Pro Ser Asp Leu Arg Arg Pro Gly His

225 230 235 240

Ile Phe Pro Leu Lys Tyr Arg Asn Gly Gly Val Leu Lys Arg Ala Gly

245 250 255

His Thr Glu Ala Ser Val Asp Leu Val Ala Leu Ala Gly Leu Arg Pro

260 265 270

Val Ser Val Leu Ser Thr Val Met Asp Pro Arg Asp Gly Ser Met Ala

275 280 285

Gly Val Thr Val Leu Gln Gln Met Ala Met Glu His Asp Ile Pro Ile

290 295 300

Val Ser Ile Ala Asp Leu Ile Arg Tyr Arg Arg Lys Arg Glu Lys Leu

305 310 315 320

Val Glu Leu Ile Ala Val Ser Arg Leu Pro Thr Lys Trp Gly Leu Phe

325 330 335

Arg Ala Tyr Cys Tyr Gln Ser Arg Leu Asp Gly Thr Glu His Ile Ala

340 345 350

Val Val Lys Gly Asp Ile Gly Asp Gly Glu Glu Val Leu Val Arg Val

355 360 365

His Ser Glu Cys Leu Thr Gly Asp Ile Leu Gly Ser Ala Arg Cys Asp

370 375 380

Cys Gly Asn Gln Leu Glu Leu Ala Met Gln Leu Ile Glu Lys Ala Gly

385 390 395 400

Arg Gly Val Leu Val Tyr Leu Arg Gly His Glu Gly Arg Gly Ile Gly

405 410 415

Leu Gly Gln Lys Leu Arg Ala Tyr Asn Leu Gln Asp Glu Gly His Asp

420 425 430

Thr Val Glu Ala Asn Val Glu Leu Gly Leu Ala Val Asp Ala Arg Glu

435 440 445

Tyr Gly Ile Gly Ala Gln Ile Leu Arg Asp Ile Gly Val Arg Thr Met

450 455 460

Arg Leu Met Thr Asn Asn Pro Ala Lys Phe Ile Gly Leu Lys Gly Tyr

465 470 475 480

Gly Leu Ala Val Val Gly Arg Val Pro Val Ile Ser Pro Ile Thr Lys

485 490 495

Glu Asn Gln Lys Tyr Leu Glu Thr Lys Arg Thr Lys Met Gly His Val

500 505 510

›SEQUENCE LISTING · 16 of 53

Tyr Gly Ser Asp Leu Pro Gly Ser Leu Pro Glu Phe Ala Asn Pro Gln

515 520 525

Glu Ala Pro Thr Glu Gln Asp Asp Asp Asp Asn Gln Asn

530 535 540

<210> SEQ ID NO 67

<211> LENGTH: 1239

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 67

gcacgagctt acacctgact ctaagcctga agacttcaat cggcctggtc atattttccc 60

tctgaaatac agagagggtg gtgttctgaa acgagctgga catactgaag catctgtaga 120

tcttgctatg ttagctgggt tacctcctgc tgcggtctta tgtgagattg tggatgagga 180

tgggtccatg gctcgcctac caaagctgcg cgtgtttgct gaaagggaga atttgaagat 240

tgtatccatt gctgacttga ttagatatag gagaaaaaga gacaggctag ttgaacgttc 300

atctgttgca cgcttacctt tgagatgggg taatgtccgc gcttactgct acagatctgt 360

tattgatggg atagagcata ttgcaatggt gaaaggggag attggagatg gtcaaggcgt 420

tttagtgagg gtgcactccg aatgcctcac aggggacatt tttggatcag caagatgtga 480

ttgtggtgat cagcttgcca tggcaatgga gatgattgag aaggctggaa ggggtgtact 540

agtttatctt cgtggacatg aagggagggg tattggtctc ggtcacaagc tccgtgctta 600

taacctgcaa gatgacgggc gcgacactgt ggaggctaat gaagacctag gactgcctgt 660

ggattcacgg gaatatggca ttggtgcaca gatattacgt gatctggggg tccgttcaat 720

gaagttgatg actaataacc cagcaaagta tggtggtctc aagggttatg gattgagcat 780

tgtgggcagg gttcccttgg taactccaat cacctcagag aatcggagat acctggaaac 840

aaagagaaca aagatggggc atgtatatgg attggccaat ggccaagcta gccatcagac 900

gggcagcaat ggtgccaaag gggagcatta ggccaatcgg actcaggttc acattctaat 960

ccacgacaat cttatgtgat ggagtttgtc aaattatgct acaacatatg ttcgtggtga 1020

accaattttt ttttagctga cagtaggttg aaagatgggc gtaataaaag tatcccttta 1080

tttgttgtaa tccacagcca cacaactggg ttattactgt aaatatatac acacacataa 1140

actgctccct agcgggtaca gactccagcc ttacctgaat tgagaagact ggcgataaaa 1200

atatcgactg agatccaagg caaaaaaaaa aaaaaaaaa 1239

<210> SEQ ID NO 68

<211> LENGTH: 309

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 68

His Glu Leu Thr Pro Asp Ser Lys Pro Glu Asp Phe Asn Arg Pro Gly

1 5 10 15

His Ile Phe Pro Leu Lys Tyr Arg Glu Gly Gly Val Leu Lys Arg Ala

20 25 30

Gly His Thr Glu Ala Ser Val Asp Leu Ala Met Leu Ala Gly Leu Pro

35 40 45

Pro Ala Ala Val Leu Cys Glu Ile Val Asp Glu Asp Gly Ser Met Ala

50 55 60

Arg Leu Pro Lys Leu Arg Val Phe Ala Glu Arg Glu Asn Leu Lys Ile

65 70 75 80

Val Ser Ile Ala Asp Leu Ile Arg Tyr Arg Arg Lys Arg Asp Arg Leu

85 90 95

Val Glu Arg Ser Ser Val Ala Arg Leu Pro Leu Arg Trp Gly Asn Val

100 105 110

Arg Ala Tyr Cys Tyr Arg Ser Val Ile Asp Gly Ile Glu His Ile Ala

115 120 125

Met Val Lys Gly Glu Ile Gly Asp Gly Gln Gly Val Leu Val Arg Val

130 135 140

His Ser Glu Cys Leu Thr Gly Asp Ile Phe Gly Ser Ala Arg Cys Asp

145 150 155 160

Cys Gly Asp Gln Leu Ala Met Ala Met Glu Met Ile Glu Lys Ala Gly

165 170 175

Arg Gly Val Leu Val Tyr Leu Arg Gly His Glu Gly Arg Gly Ile Gly

180 185 190

Leu Gly His Lys Leu Arg Ala Tyr Asn Leu Gln Asp Asp Gly Arg Asp

195 200 205

Thr Val Glu Ala Asn Glu Asp Leu Gly Leu Pro Val Asp Ser Arg Glu

210 215 220

Tyr Gly Ile Gly Ala Gln Ile Leu Arg Asp Leu Gly Val Arg Ser Met

225 230 235 240

Lys Leu Met Thr Asn Asn Pro Ala Lys Tyr Gly Gly Leu Lys Gly Tyr

245 250 255

Gly Leu Ser Ile Val Gly Arg Val Pro Leu Val Thr Pro Ile Thr Ser

260 265 270

Glu Asn Arg Arg Tyr Leu Glu Thr Lys Arg Thr Lys Met Gly His Val

275 280 285

Tyr Gly Leu Ala Asn Gly Gln Ala Ser His Gln Thr Gly Ser Asn Gly

290 295 300

Ala Lys Gly Glu His

305

<210> SEQ ID NO 69

<211> LENGTH: 1636

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 69

acgtcgccat ggcctaccat acgacatacc aagattacgc tcatatggcc atggaggcca 60

gtgaatgatc ctttaaagga aagcaataat ggacctgttt tgcaaggatt caatggatct 120

agtgcttcct ttcggactgt tggtgctaaa atcactcagg aaactggtga tttctttgtt 180

agcgatgcag agggtgaccc agacaaacca actgatggtt tttcctctat tgatgaggct 240

ataggcgcat tgcatgaagg aaagtttgtt attgctgtag atgatgaaag cggtgataat 300

gaaggggatc ttgtcatggc agctacgctg gcagacccag aatctattgc attcatgatc 360

aggaatggtt ctgggatcat ctcagtgggc atgaaggaag aggacttaac aagattgatg 420

attcctatga tgtctccaat tgcagaaatt gaggatattt cagctgctgc ttccacagta 480

acagtggatg ccagagtggg catatcaacc ggcgtctcgg ctgcagatag ggcaaaaacg 540

atttttactc tagcctcccc tgattctaag ccaactgacc tcagaagacc aggccatata 600

ttccctctaa aataccgaaa cggtggtgtg ctaaaaagag ctggacatac tgaggcatcc 660

gtcgatcttg tcgcgttggc tggcttgcgc cctgtgtctg tcctgtcaac cgtcatcaac 720

ccagtggatg gttcaatggc aggaatgcca gtgctgaaac agatggcttt ggagcatgat 780

atcccaattg tttcaatcgc tgatctcatc cggtatagaa ggaagaggga gaagctggtg 840

gaactgattg ctgtatctcg tttgccgacg aaatggggcc ttttccgagc ttactgctac 900

caatccaagc ttgatggaac cgagcacatt gctgttgcaa agggcgacat cggcgacggc 960

gaggacgtct tggtgagggt ccattccgag tgcctgaccg gcgacatcct cggctccgcc 1020

cgctgcgact gcggcaacca gctggacctg gcgatgcagc tcatcgacaa ggccggccgc 1080

ggcgtcctcg tctacctccg cggccacgag ggccgcggca tcggcctcgg ccagaagctc 1140

cgcgcctaca acctccagga cgacggccac gacaccgtcc aggccaacgt cgagctcggc 1200

ctcgccgtcg actcccgcga gtacggcatc ggcgcccaga ttctgcggga catgggggtg 1260

cgcacgatgc ggctgatgac gaacaacccg gcaaagttcg tcgggctcaa gggctacggg 1320

ctcgccgtcg tcggcagggt tccggtgatc tcccccatca ccaaggagaa ccagaggtac 1380

ctcgagacga agcgcaccaa gatgggccat gtctacggct ccgacctccc cggcaacgtc 1440

ccggaggaat tcctcaaccc ggacgacatc gccggagacc aagacgaaga cgacacccac 1500

aactgaatgc aacaacaaaa ttcacaacca cagattctcc acatctcatt ttgtgatcaa 1560

attgcatctc aaactgcacc gatcttgtta tactagtaac aaatcttgat ttggaaccaa 1620

aaaaaaaaaa aaaaaa 1636

<210> SEQ ID NO 70

<211> LENGTH: 501

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 70

Thr Ser Pro Trp Pro Thr Ile Arg His Thr Lys Ile Thr Leu Ile Trp

1 5 10 15

Pro Trp Arg Pro Val Asn Asp Pro Leu Lys Glu Ser Asn Asn Gly Pro

20 25 30

Val Leu Gln Gly Phe Asn Gly Ser Ser Ala Ser Phe Arg Thr Val Gly

35 40 45

Ala Lys Ile Thr Gln Glu Thr Gly Asp Phe Phe Val Ser Asp Ala Glu

50 55 60

Gly Asp Pro Asp Lys Pro Thr Asp Gly Phe Ser Ser Ile Asp Glu Ala

65 70 75 80

Ile Gly Ala Leu His Glu Gly Lys Phe Val Ile Ala Val Asp Asp Glu

85 90 95

Ser Gly Asp Asn Glu Gly Asp Leu Val Met Ala Ala Thr Leu Ala Asp

100 105 110

Pro Glu Ser Ile Ala Phe Met Ile Arg Asn Gly Ser Gly Ile Ile Ser

115 120 125

Val Gly Met Lys Glu Glu Asp Leu Thr Arg Leu Met Ile Pro Met Met

130 135 140

Ser Pro Ile Ala Glu Ile Glu Asp Ile Ser Ala Ala Ala Ser Thr Val

145 150 155 160

Thr Val Asp Ala Arg Val Gly Ile Ser Thr Gly Val Ser Ala Ala Asp

›SEQUENCE LISTING · 17 of 53

165 170 175

Arg Ala Lys Thr Ile Phe Thr Leu Ala Ser Pro Asp Ser Lys Pro Thr

180 185 190

Asp Leu Arg Arg Pro Gly His Ile Phe Pro Leu Lys Tyr Arg Asn Gly

195 200 205

Gly Val Leu Lys Arg Ala Gly His Thr Glu Ala Ser Val Asp Leu Val

210 215 220

Ala Leu Ala Gly Leu Arg Pro Val Ser Val Leu Ser Thr Val Ile Asn

225 230 235 240

Pro Val Asp Gly Ser Met Ala Gly Met Pro Val Leu Lys Gln Met Ala

245 250 255

Leu Glu His Asp Ile Pro Ile Val Ser Ile Ala Asp Leu Ile Arg Tyr

260 265 270

Arg Arg Lys Arg Glu Lys Leu Val Glu Leu Ile Ala Val Ser Arg Leu

275 280 285

Pro Thr Lys Trp Gly Leu Phe Arg Ala Tyr Cys Tyr Gln Ser Lys Leu

290 295 300

Asp Gly Thr Glu His Ile Ala Val Ala Lys Gly Asp Ile Gly Asp Gly

305 310 315 320

Glu Asp Val Leu Val Arg Val His Ser Glu Cys Leu Thr Gly Asp Ile

325 330 335

Leu Gly Ser Ala Arg Cys Asp Cys Gly Asn Gln Leu Asp Leu Ala Met

340 345 350

Gln Leu Ile Asp Lys Ala Gly Arg Gly Val Leu Val Tyr Leu Arg Gly

355 360 365

His Glu Gly Arg Gly Ile Gly Leu Gly Gln Lys Leu Arg Ala Tyr Asn

370 375 380

Leu Gln Asp Asp Gly His Asp Thr Val Gln Ala Asn Val Glu Leu Gly

385 390 395 400

Leu Ala Val Asp Ser Arg Glu Tyr Gly Ile Gly Ala Gln Ile Leu Arg

405 410 415

Asp Met Gly Val Arg Thr Met Arg Leu Met Thr Asn Asn Pro Ala Lys

420 425 430

Phe Val Gly Leu Lys Gly Tyr Gly Leu Ala Val Val Gly Arg Val Pro

435 440 445

Val Ile Ser Pro Ile Thr Lys Glu Asn Gln Arg Tyr Leu Glu Thr Lys

450 455 460

Arg Thr Lys Met Gly His Val Tyr Gly Ser Asp Leu Pro Gly Asn Val

465 470 475 480

Pro Glu Glu Phe Leu Asn Pro Asp Asp Ile Ala Gly Asp Gln Asp Glu

485 490 495

Asp Asp Thr His Asn

500

<210> SEQ ID NO 71

<211> LENGTH: 2079

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 71

gcacgagatt cattcattga caaaacaaaa caatggcttc tttcaatctc tcttattctt 60

cctcaccagc cctctcacgt ccccgggcaa gcaaacactt caaattcttc aatgatgtgc 120

cttttgtccg aatgaattct aaatttccct caagtgataa tgcttttggg agaatcaaag 180

ctatgttgat ctctggaggg ggtgaactac tttcccatcc aaatggcaat gacattgtga 240

taggtacaaa tctggttgga gatgaatctg ttcaaattga ggtacagcct aatggaacac 300

tggcagatga cacagggcta acaagcaatg ttttctctat ggacaacaat gaatttgatt 360

cggacagccc ctcagcaggt tttgcttcca ttcctgaggc aattgaagac attcgccagg 420

gaaagatggt agtggttgtg gacgatgaag atcgagaaaa tgaaggagac ttgataatgg 480

cagcacagtt ggcaacaccc gaggcaatgg cttttattgt aaagcatgga actggtatag 540

tttgtgtaag catgaaagag gaagatctgg atagattgga acttcctttg atggtgaaca 600

gtaaggacaa tgatgagaaa ctttgtacgg cattcactgt gacagtggat gctaaacatg 660

gtacaaccac tggggtgtca gctcacgata gggcaacaac agtcttggcc cttgcgtcta 720

gagattctac tccaggcgat ttcaatcgcc cgggccatat tttcccacta aaatacaggg 780

aaggtggtgt cttgaagaga gccggacata cagaagcttc tgttgatctt gctgtacttg 840

ctggtttgga tccagtggca gttctgtgtg agattgtaga tgatgatggt tccatggcta 900

gactgccaaa gcttcgccag tttgcagagc atgaaaattt gaaaattgta tctattgctg 960

acttaataag atatagaaga aaaagggata agttagtgga tcgctcttct gctgcacaga 1020

tacctacaat gtggggacca ttcacagctt actgttacag atcactttta gacgggattg 1080

agcatattgc aatggttaag ggtgacattg gagatggaca agatgttctt gtgagagtac 1140

actcagagtg tctgacagga gacatatttg gatctgccag atgtgactgc ggaaatcagc 1200

ttgcacttgc aatgcaacag attgaggctg ctggtaaggg tgtgctggta tatctccgtg 1260

gacacgaagg aaggggtatt ggattgggcc acaagcttcg tgcttataac ctacaggatg 1320

atggacgaga tactgttgaa gccaatgagg aattgggatt gccagttgac tccagggagt 1380

atggcattgg tgcacagatg ttgagggact tgggtgtacg atctatgaag ctgatgacaa 1440

acaatccggc aaaatatatt gggctcaaag gttatggttt gacagtttcg ggtaggatcc 1500

cattgttaac tcttatcact tcggagaaca agagatattt agagaccaaa cgtgtgaaaa 1560

tgggtcacat atatggcacg gaatttaacg gccgattgag cactcatgac agtggtaatg 1620

gtaatgccac caggattgag gactctaatg ctgttactgg cttataaaca tccttttttt 1680

aactagataa tatggaagaa ctagatttcc atggtgcaga tgaatacata aagttataaa 1740

gttatagaaa ccatttttct tattcatttg tctgataaac ttcagctcta ctaaactata 1800

ggctctaccg aacaccattc atcttgatta ttgaaacata gtgccatgac aagggcattg 1860

ctatttcagg gttatttttt ctttggtgcc ttttagaggt tcatcatttc tagactctgg 1920

aaatcattag ccgatttaaa cctagtttct tcattctttt ttatccaatt acccatgcat 1980

cacagttgcc tcaataatgc atgatagaaa agttatatga tttgtagctc cacaatttat 2040

aataaataaa ttccttttgt aaaaaaaaaa aaaaaaaaa 2079

<210> SEQ ID NO 72

<211> LENGTH: 544

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 72

Met Ala Ser Phe Asn Leu Ser Tyr Ser Ser Ser Pro Ala Leu Ser Arg

1 5 10 15

Pro Arg Ala Ser Lys His Phe Lys Phe Phe Asn Asp Val Pro Phe Val

20 25 30

Arg Met Asn Ser Lys Phe Pro Ser Ser Asp Asn Ala Phe Gly Arg Ile

35 40 45

Lys Ala Met Leu Ile Ser Gly Gly Gly Glu Leu Leu Ser His Pro Asn

50 55 60

Gly Asn Asp Ile Val Ile Gly Thr Asn Leu Val Gly Asp Glu Ser Val

65 70 75 80

Gln Ile Glu Val Gln Pro Asn Gly Thr Leu Ala Asp Asp Thr Gly Leu

85 90 95

Thr Ser Asn Val Phe Ser Met Asp Asn Asn Glu Phe Asp Ser Asp Ser

100 105 110

Pro Ser Ala Gly Phe Ala Ser Ile Pro Glu Ala Ile Glu Asp Ile Arg

115 120 125

Gln Gly Lys Met Val Val Val Val Asp Asp Glu Asp Arg Glu Asn Glu

130 135 140

Gly Asp Leu Ile Met Ala Ala Gln Leu Ala Thr Pro Glu Ala Met Ala

145 150 155 160

Phe Ile Val Lys His Gly Thr Gly Ile Val Cys Val Ser Met Lys Glu

165 170 175

Glu Asp Leu Asp Arg Leu Glu Leu Pro Leu Met Val Asn Ser Lys Asp

180 185 190

Asn Asp Glu Lys Leu Cys Thr Ala Phe Thr Val Thr Val Asp Ala Lys

195 200 205

His Gly Thr Thr Thr Gly Val Ser Ala His Asp Arg Ala Thr Thr Val

210 215 220

Leu Ala Leu Ala Ser Arg Asp Ser Thr Pro Gly Asp Phe Asn Arg Pro

225 230 235 240

Gly His Ile Phe Pro Leu Lys Tyr Arg Glu Gly Gly Val Leu Lys Arg

245 250 255

Ala Gly His Thr Glu Ala Ser Val Asp Leu Ala Val Leu Ala Gly Leu

260 265 270

Asp Pro Val Ala Val Leu Cys Glu Ile Val Asp Asp Asp Gly Ser Met

275 280 285

Ala Arg Leu Pro Lys Leu Arg Gln Phe Ala Glu His Glu Asn Leu Lys

290 295 300

Ile Val Ser Ile Ala Asp Leu Ile Arg Tyr Arg Arg Lys Arg Asp Lys

305 310 315 320

Leu Val Asp Arg Ser Ser Ala Ala Gln Ile Pro Thr Met Trp Gly Pro

325 330 335

Phe Thr Ala Tyr Cys Tyr Arg Ser Leu Leu Asp Gly Ile Glu His Ile

340 345 350

Ala Met Val Lys Gly Asp Ile Gly Asp Gly Gln Asp Val Leu Val Arg

355 360 365

Val His Ser Glu Cys Leu Thr Gly Asp Ile Phe Gly Ser Ala Arg Cys

370 375 380

Asp Cys Gly Asn Gln Leu Ala Leu Ala Met Gln Gln Ile Glu Ala Ala

385 390 395 400

Gly Lys Gly Val Leu Val Tyr Leu Arg Gly His Glu Gly Arg Gly Ile

›SEQUENCE LISTING · 18 of 53

405 410 415

Gly Leu Gly His Lys Leu Arg Ala Tyr Asn Leu Gln Asp Asp Gly Arg

420 425 430

Asp Thr Val Glu Ala Asn Glu Glu Leu Gly Leu Pro Val Asp Ser Arg

435 440 445

Glu Tyr Gly Ile Gly Ala Gln Met Leu Arg Asp Leu Gly Val Arg Ser

450 455 460

Met Lys Leu Met Thr Asn Asn Pro Ala Lys Tyr Ile Gly Leu Lys Gly

465 470 475 480

Tyr Gly Leu Thr Val Ser Gly Arg Ile Pro Leu Leu Thr Leu Ile Thr

485 490 495

Ser Glu Asn Lys Arg Tyr Leu Glu Thr Lys Arg Val Lys Met Gly His

500 505 510

Ile Tyr Gly Thr Glu Phe Asn Gly Arg Leu Ser Thr His Asp Ser Gly

515 520 525

Asn Gly Asn Ala Thr Arg Ile Glu Asp Ser Asn Ala Val Thr Gly Leu

530 535 540

<210> SEQ ID NO 73

<211> LENGTH: 934

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 73

gcacgagatc tgtcctttca accatcatcg acccgaagga cggctcgatg gcatcaacac 60

aagcgctgaa acagatggct ttggagcatg atgtcccgat tgtttcgatc gctgatctca 120

tccggtacag gaggaaaagg gagaagctgg tggaattgat cgctgtatct cgtttgccta 180

ctaaatgggg cctattccgt gcttactgct accaatccaa gctggatgga actgagcaca 240

tcgctgttgc aaagggtgat attggcgatg gagaagacgt gctggtgagg gtgcattcag 300

agtgcctgac cggcgacatc ctcggctccg cccgctgcga ctgtggcgag cagctggacc 360

tggcgatgca gctgatcgag aaggccggcc gcggcgtcct cgtgtaccta cgcggacatg 420

aaggccgagg catcgggctc ggccagaagc tccgcgccta caacctgcag gaccaaggca 480

gcgacacggt ggaggccaac gtcgagctcg gcctcgccat cgactcccgc gagtacggca 540

tcggtgccca gattctcagg gacattggcg tgcgcacgat gcggctgatg acgaacaacc 600

cggccaaatt cgtggggctc aaggggtacg ggctcgcggt ggtgggccgg gtcccggtga 660

tctcgccgat cacaaaggag aaccagaaat acctcgagac caagaggacc aagatggggc 720

acctctacgg ctccgacctc cccggcggcc tcctcaagga attcctcaac cctacggaag 780

acaacgccac gaactaatta tacacccacc acacgatcca cggatcattt ggtttctttt 840

aacttcattt ctttctgata tacaacgatc gaacgagaat tgagacgaaa tattgcaacc 900

aatctgtagc gattccaaaa aaaaaaaaaa aaaa 934

<210> SEQ ID NO 74

<211> LENGTH: 264

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 74

Thr Arg Ser Val Leu Ser Thr Ile Ile Asp Pro Lys Asp Gly Ser Met

1 5 10 15

Ala Ser Thr Gln Ala Leu Lys Gln Met Ala Leu Glu His Asp Val Pro

20 25 30

Ile Val Ser Ile Ala Asp Leu Ile Arg Tyr Arg Arg Lys Arg Glu Lys

35 40 45

Leu Val Glu Leu Ile Ala Val Ser Arg Leu Pro Thr Lys Trp Gly Leu

50 55 60

Phe Arg Ala Tyr Cys Tyr Gln Ser Lys Leu Asp Gly Thr Glu His Ile

65 70 75 80

Ala Val Ala Lys Gly Asp Ile Gly Asp Gly Glu Asp Val Leu Val Arg

85 90 95

Val His Ser Glu Cys Leu Thr Gly Asp Ile Leu Gly Ser Ala Arg Cys

100 105 110

Asp Cys Gly Glu Gln Leu Asp Leu Ala Met Gln Leu Ile Glu Lys Ala

115 120 125

Gly Arg Gly Val Leu Val Tyr Leu Arg Gly His Glu Gly Arg Gly Ile

130 135 140

Gly Leu Gly Gln Lys Leu Arg Ala Tyr Asn Leu Gln Asp Gln Gly Ser

145 150 155 160

Asp Thr Val Glu Ala Asn Val Glu Leu Gly Leu Ala Ile Asp Ser Arg

165 170 175

Glu Tyr Gly Ile Gly Ala Gln Ile Leu Arg Asp Ile Gly Val Arg Thr

180 185 190

Met Arg Leu Met Thr Asn Asn Pro Ala Lys Phe Val Gly Leu Lys Gly

195 200 205

Tyr Gly Leu Ala Val Val Gly Arg Val Pro Val Ile Ser Pro Ile Thr

210 215 220

Lys Glu Asn Gln Lys Tyr Leu Glu Thr Lys Arg Thr Lys Met Gly His

225 230 235 240

Leu Tyr Gly Ser Asp Leu Pro Gly Gly Leu Leu Lys Glu Phe Leu Asn

245 250 255

Pro Thr Glu Asp Asn Ala Thr Asn

260

<210> SEQ ID NO 75

<211> LENGTH: 970

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 75

gcacgagggc atccaaaggg attgagaagc tccaaggtgc tgggataagt gtgagggtgg 60

gtgtggagga agcgttatgc cgcaaactaa acgaggctta tatccatcgt atgctcaccg 120

ggaaggcttt tgcaactctg agagctacac tctcaatgaa tggaatcatc acgaaccaga 180

ttgggaaggg agctgatcag tcaggtgggt actactcgca gctgatgaaa gaatacgacg 240

gagtcataat ttcaagcgac ctggccaaga tgtcggcctt acctttgtcg cgtgaagccg 300

gcacaaacca acctctctgt atcatcatag cacagggtga aagctcccgg ctacacatcc 360

catctctcag ccaagagcat gcatccaggg ccatagtcct tgccgatagt ccggtcactg 420

tggagccagc aggagtggaa gttgcagttt ttcgtcagat agacctggag tctattctcc 480

agcttcttgc gcagcgaggg ctctgcagcg tgctggtgga cttcagagag gccggcgaaa 540

gcttcgcgtc gcttctgaac gacttccagg aggacaagct ggtgcagaag gtcgtcgttg 600

aggtcctgcc cttttggctc gcgagcgacg ggctcagcaa cctggcattt ggtggcagcc 660

aatcgttccc gttgaagaac ttggagctca gggatgtgaa tgggagtgtg ctgctggaag 720

gctatgtgta atagcctctc ttctgtcgga gattggcatt gtttgttttc cctcgtgtaa 780

acagatagcc ctgtaatata cttgcagagc cgcccacgag ccatgtgggg ttttgctttt 840

ttatatatat agttgaaaat caatgccatg cgtccttcat tttgctgaca tggaactgct 900

gcccggtgat actccctgtc tgcaaatcaa tgcaaatgtc ttattaaaaa aaaaaaaaaa 960

aaaaaaaaaa 970

<210> SEQ ID NO 76

<211> LENGTH: 242

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 76

Thr Arg Ala Ser Lys Gly Ile Glu Lys Leu Gln Gly Ala Gly Ile Ser

1 5 10 15

Val Arg Val Gly Val Glu Glu Ala Leu Cys Arg Lys Leu Asn Glu Ala

20 25 30

Tyr Ile His Arg Met Leu Thr Gly Lys Ala Phe Ala Thr Leu Arg Ala

35 40 45

Thr Leu Ser Met Asn Gly Ile Ile Thr Asn Gln Ile Gly Lys Gly Ala

50 55 60

Asp Gln Ser Gly Gly Tyr Tyr Ser Gln Leu Met Lys Glu Tyr Asp Gly

65 70 75 80

Val Ile Ile Ser Ser Asp Leu Ala Lys Met Ser Ala Leu Pro Leu Ser

85 90 95

Arg Glu Ala Gly Thr Asn Gln Pro Leu Cys Ile Ile Ile Ala Gln Gly

100 105 110

Glu Ser Ser Arg Leu His Ile Pro Ser Leu Ser Gln Glu His Ala Ser

115 120 125

Arg Ala Ile Val Leu Ala Asp Ser Pro Val Thr Val Glu Pro Ala Gly

130 135 140

Val Glu Val Ala Val Phe Arg Gln Ile Asp Leu Glu Ser Ile Leu Gln

145 150 155 160

Leu Leu Ala Gln Arg Gly Leu Cys Ser Val Leu Val Asp Phe Arg Glu

165 170 175

Ala Gly Glu Ser Phe Ala Ser Leu Leu Asn Asp Phe Gln Glu Asp Lys

180 185 190

Leu Val Gln Lys Val Val Val Glu Val Leu Pro Phe Trp Leu Ala Ser

195 200 205

Asp Gly Leu Ser Asn Leu Ala Phe Gly Gly Ser Gln Ser Phe Pro Leu

210 215 220

Lys Asn Leu Glu Leu Arg Asp Val Asn Gly Ser Val Leu Leu Glu Gly

225 230 235 240

Tyr Val

<210> SEQ ID NO 77

<211> LENGTH: 1445

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 77

gcacgaggtt ctaacctcct ctgcgcgacg gacatggcct cctcgctcgt ctcgcgcacc 60

cacctcactc cgcgccccgc cgccgccgcc tcgacggcct ctccttgcag cgcccgcttc 120

gcggtccggc ggcgccgtgg cctcgtcggc ggtgtgcggt gccaggcgca ggcgagcgac 180

atggacggcc actacatgcg gcggtgcgtg gagctggcgc gcaaggcggc cggacacacc 240

agccctaacc ccatggtcgg ctgtgtcgtt gtccgcgacg gccgcgtcgt cggcgaggga 300

ttccatccca aagccggcca gccccacgcc gaggtgtttg ctctgagaga tgctggggat 360

›SEQUENCE LISTING · 19 of 53

ttagcagaga atgcaacagc ctatgtgagc ttagagccct gcaaccatta cgggcggaca 420

cctccctgca ctgaagcact catcaatgcc aaagttaaag atgttgtggt ggggatgact 480

gacccaaatc ctattgtggc atccaagggg attgaaaggc tcagaagcgc cgggatagat 540

gtgaggttgt gcgtggatga agaagcgtca tgccgcaagc taaatgaggc ttacatccat 600

cgcatgctta ctgggaaggc ctttgcaact ttaagaacta cactctcgat gaatggagtt 660

gtcatcaatc agattgggag tggagctgat caaccagaag gatactactc gcagttattg 720

aaagaatacg atggagtaat aatttcaagc aattttgcca agacaaatac cttgcctgta 780

tcccgtgaag ctggtgcgaa gcagcctctt tatatcatca tagcgcagtg cgaaaattcc 840

caattaaaca ttccgtttct caaagaagag cgtgcagccg aggcagtagt tctgtcggat 900

agtcctatca ctgtggagcc agcaggagtt gatgttttag tccttgatca gatgaacctg 960

gatgccattc ttcaacttct tgcacaacga gggctatgta gtgtgctggt ggatttcagg 1020

gaggctggag gaggcattgc atcacttctg aacaattttc aggatgacaa gctggtacag 1080

aaggttgttg tggagctcct gcctgtctgg gcggtcagca aagggccgag cgacctggcg 1140

tttggtggga gccagtcatt tccactgaag gatgtggagc acagtgaggt aaatggatcc 1200

gtgctgcttc agggctatgt gtaacaaact tttttggttc gaggttgatc ctgccctgag 1260

tagcgctgca tatttttacc tctcactctc tctcgttctg gctctggtaa actgtccgtc 1320

atgtaaactt acctgaatct gcagtcaatg cggtggtgct cctagacagg gatctggttc 1380

ttctgatttt tacagtatca tgcaataaat ctatgagaga actgcgaaaa aaaaaaaaaa 1440

aaaaa 1445

<210> SEQ ID NO 78

<211> LENGTH: 407

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 78

Ala Arg Gly Ser Asn Leu Leu Cys Ala Thr Asp Met Ala Ser Ser Leu

1 5 10 15

Val Ser Arg Thr His Leu Thr Pro Arg Pro Ala Ala Ala Ala Ser Thr

20 25 30

Ala Ser Pro Cys Ser Ala Arg Phe Ala Val Arg Arg Arg Arg Gly Leu

35 40 45

Val Gly Gly Val Arg Cys Gln Ala Gln Ala Ser Asp Met Asp Gly His

50 55 60

Tyr Met Arg Arg Cys Val Glu Leu Ala Arg Lys Ala Ala Gly His Thr

65 70 75 80

Ser Pro Asn Pro Met Val Gly Cys Val Val Val Arg Asp Gly Arg Val

85 90 95

Val Gly Glu Gly Phe His Pro Lys Ala Gly Gln Pro His Ala Glu Val

100 105 110

Phe Ala Leu Arg Asp Ala Gly Asp Leu Ala Glu Asn Ala Thr Ala Tyr

115 120 125

Val Ser Leu Glu Pro Cys Asn His Tyr Gly Arg Thr Pro Pro Cys Thr

130 135 140

Glu Ala Leu Ile Asn Ala Lys Val Lys Asp Val Val Val Gly Met Thr

145 150 155 160

Asp Pro Asn Pro Ile Val Ala Ser Lys Gly Ile Glu Arg Leu Arg Ser

165 170 175

Ala Gly Ile Asp Val Arg Leu Cys Val Asp Glu Glu Ala Ser Cys Arg

180 185 190

Lys Leu Asn Glu Ala Tyr Ile His Arg Met Leu Thr Gly Lys Ala Phe

195 200 205

Ala Thr Leu Arg Thr Thr Leu Ser Met Asn Gly Val Val Ile Asn Gln

210 215 220

Ile Gly Ser Gly Ala Asp Gln Pro Glu Gly Tyr Tyr Ser Gln Leu Leu

225 230 235 240

Lys Glu Tyr Asp Gly Val Ile Ile Ser Ser Asn Phe Ala Lys Thr Asn

245 250 255

Thr Leu Pro Val Ser Arg Glu Ala Gly Ala Lys Gln Pro Leu Tyr Ile

260 265 270

Ile Ile Ala Gln Cys Glu Asn Ser Gln Leu Asn Ile Pro Phe Leu Lys

275 280 285

Glu Glu Arg Ala Ala Glu Ala Val Val Leu Ser Asp Ser Pro Ile Thr

290 295 300

Val Glu Pro Ala Gly Val Asp Val Leu Val Leu Asp Gln Met Asn Leu

305 310 315 320

Asp Ala Ile Leu Gln Leu Leu Ala Gln Arg Gly Leu Cys Ser Val Leu

325 330 335

Val Asp Phe Arg Glu Ala Gly Gly Gly Ile Ala Ser Leu Leu Asn Asn

340 345 350

Phe Gln Asp Asp Lys Leu Val Gln Lys Val Val Val Glu Leu Leu Pro

355 360 365

Val Trp Ala Val Ser Lys Gly Pro Ser Asp Leu Ala Phe Gly Gly Ser

370 375 380

Gln Ser Phe Pro Leu Lys Asp Val Glu His Ser Glu Val Asn Gly Ser

385 390 395 400

Val Leu Leu Gln Gly Tyr Val

405

<210> SEQ ID NO 79

<211> LENGTH: 866

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 79

cccggcgact gctacgggga cagcaccgcc gtctcctccc tcgtgcagtc aggaattaca 60

agagttgtgg tgggtcttag acatcccttg aagcacttga gagggaaagc gattcaatca 120

ttgcgaagtg aaggcattca ggtcgatatt gtgggggaag atctccagag caaattgttc 180

aaggaagcct ttacttcatg cctcatcgta aatgctccat tactttaccg agctgccttt 240

cgtgttccat tctctgttct gaagtatgct atgactgcag atggaaaaat tgcagctagt 300

agtgggcatg cgtcttgggt aagcggcaga gcatccagag gccgagtatt tgaattgcgt 360

ggaagaagtg atgctgttat agttggtggg aatacagttc gccgtgatga tcctcgatta 420

actgcaaggc atgtgaaggg gcatgttcca gtgcgcattg taatgtcaca aacttgtaac 480

cttcctgaag aagcaaattt atggaatgtt catgaggcct atacaatagt tgcaacacaa 540

agaggtgctc ggagagattt ccaaaagaag cttgctatga agggtgttga agtagtggaa 600

tttgacatgc taaatcctag agatgttatg tcatattgtt atgatcgtgg ttacctttct 660

gtattatggg agtgcggtgg gaccctatca gctgctgcta tatctgcaag agttattcac 720

aaggtttatg cattctgtgc tccaaaaata atcggtggag taactgctcc aacaccagta 780

ggtgacctag ggatgaatca aatgactcag gcgattgatt taattgatgt ttcacatgag 840

catgtcacgg tcaatctgcc tcgtgc 866

<210> SEQ ID NO 80

<211> LENGTH: 288

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 80

Pro Gly Asp Cys Tyr Gly Asp Ser Thr Ala Val Ser Ser Leu Val Gln

1 5 10 15

Ser Gly Ile Thr Arg Val Val Val Gly Leu Arg His Pro Leu Lys His

20 25 30

Leu Arg Gly Lys Ala Ile Gln Ser Leu Arg Ser Glu Gly Ile Gln Val

35 40 45

Asp Ile Val Gly Glu Asp Leu Gln Ser Lys Leu Phe Lys Glu Ala Phe

50 55 60

Thr Ser Cys Leu Ile Val Asn Ala Pro Leu Leu Tyr Arg Ala Ala Phe

65 70 75 80

Arg Val Pro Phe Ser Val Leu Lys Tyr Ala Met Thr Ala Asp Gly Lys

85 90 95

Ile Ala Ala Ser Ser Gly His Ala Ser Trp Val Ser Gly Arg Ala Ser

100 105 110

Arg Gly Arg Val Phe Glu Leu Arg Gly Arg Ser Asp Ala Val Ile Val

115 120 125

Gly Gly Asn Thr Val Arg Arg Asp Asp Pro Arg Leu Thr Ala Arg His

130 135 140

Val Lys Gly His Val Pro Val Arg Ile Val Met Ser Gln Thr Cys Asn

145 150 155 160

Leu Pro Glu Glu Ala Asn Leu Trp Asn Val His Glu Ala Tyr Thr Ile

165 170 175

Val Ala Thr Gln Arg Gly Ala Arg Arg Asp Phe Gln Lys Lys Leu Ala

180 185 190

Met Lys Gly Val Glu Val Val Glu Phe Asp Met Leu Asn Pro Arg Asp

195 200 205

Val Met Ser Tyr Cys Tyr Asp Arg Gly Tyr Leu Ser Val Leu Trp Glu

210 215 220

Cys Gly Gly Thr Leu Ser Ala Ala Ala Ile Ser Ala Arg Val Ile His

225 230 235 240

Lys Val Tyr Ala Phe Cys Ala Pro Lys Ile Ile Gly Gly Val Thr Ala

245 250 255

Pro Thr Pro Val Gly Asp Leu Gly Met Asn Gln Met Thr Gln Ala Ile

260 265 270

Asp Leu Ile Asp Val Ser His Glu His Val Thr Val Asn Leu Pro Arg

275 280 285

<210> SEQ ID NO 81

<211> LENGTH: 1251

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 81

gcacgagctt acaagaggac ttaacaagat tgatgattcc tatgatgtct ccaattgcag 60

aaattgagga tatttcagct gctgcttcca cagtaacagt ggatgccaga gtgggcatat 120

caaccggcgt ctcggctgca gatagggcaa aaacgatttt tactctagcc tcccctgatt 180

›SEQUENCE LISTING · 20 of 53

ctaagccaac tgacctcaga agaccaggcc atatattccc tctaaaatac cgaaacggtg 240

gtgtgctaaa aagagctgga catactgagg catccgtcga tcttgtcgcg ttggctggct 300

tgcgccctgt gtctgtcctg tcaaccgtca tcaacccagt ggatggttca atggcaggaa 360

tgccagtgct gaaacagatg gctttggagc atgatatccc aattgtttca atcgctgatc 420

tcatccggta tagaaggaag agggagaagc tggtggaact gattgctgta tctcgtttgc 480

cgacgaaatg gggccttttc cgagcttact gctaccaatc caagcttgat ggaaccgagc 540

acattgctgt tgcaaagggc gacatcggcg acggcgagga cgtcttggtg agggtccatt 600

ccgagtgcct gaccggcgac atcctcggct ccgcccgctg cgactgcggc aaccagctgg 660

acctggcgat gcagctcatc gacaaggccg gccgcggcgt cctcgtctac ctccgcggcc 720

acgagggccg cggcatcggc ctcggccaga agctccgcgc ctacaacctc caggacgacg 780

gccacgacac cgtccaggcc aacgtcgagc tcggcctcgc cgtcgactcc cgcgagtacg 840

gcatcggcgc ccagattctg cgggacatgg gggtgcgcac gatgcggctg atgacgaaca 900

acccggcaaa gttcgtcggg ctcaagggct acgggctcgc cgtcgtcggc agggttccgg 960

tgatctcccc catcaccaag gagaaccaga ggtacctcga gacgaagcgc accaagatgg 1020

gccatgtcta cggctccgac ctccccggca acgtcccgga ggaattcctc aacccggacg 1080

acatcgccgg agaccaagac gaagacgaca cccacaactg aatgcaacaa caaaattcac 1140

aaccacagat tctccacatc tcattttgtg atcaaattgc atctcaaact gcaccgatct 1200

tgttatacta gtaacaaatc ttgatttgga accaaaaaaa aaaaaaaaaa a 1251

<210> SEQ ID NO 82

<211> LENGTH: 372

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 82

Thr Ser Leu Gln Glu Asp Leu Thr Arg Leu Met Ile Pro Met Met Ser

1 5 10 15

Pro Ile Ala Glu Ile Glu Asp Ile Ser Ala Ala Ala Ser Thr Val Thr

20 25 30

Val Asp Ala Arg Val Gly Ile Ser Thr Gly Val Ser Ala Ala Asp Arg

35 40 45

Ala Lys Thr Ile Phe Thr Leu Ala Ser Pro Asp Ser Lys Pro Thr Asp

50 55 60

Leu Arg Arg Pro Gly His Ile Phe Pro Leu Lys Tyr Arg Asn Gly Gly

65 70 75 80

Val Leu Lys Arg Ala Gly His Thr Glu Ala Ser Val Asp Leu Val Ala

85 90 95

Leu Ala Gly Leu Arg Pro Val Ser Val Leu Ser Thr Val Ile Asn Pro

100 105 110

Val Asp Gly Ser Met Ala Gly Met Pro Val Leu Lys Gln Met Ala Leu

115 120 125

Glu His Asp Ile Pro Ile Val Ser Ile Ala Asp Leu Ile Arg Tyr Arg

130 135 140

Arg Lys Arg Glu Lys Leu Val Glu Leu Ile Ala Val Ser Arg Leu Pro

145 150 155 160

Thr Lys Trp Gly Leu Phe Arg Ala Tyr Cys Tyr Gln Ser Lys Leu Asp

165 170 175

Gly Thr Glu His Ile Ala Val Ala Lys Gly Asp Ile Gly Asp Gly Glu

180 185 190

Asp Val Leu Val Arg Val His Ser Glu Cys Leu Thr Gly Asp Ile Leu

195 200 205

Gly Ser Ala Arg Cys Asp Cys Gly Asn Gln Leu Asp Leu Ala Met Gln

210 215 220

Leu Ile Asp Lys Ala Gly Arg Gly Val Leu Val Tyr Leu Arg Gly His

225 230 235 240

Glu Gly Arg Gly Ile Gly Leu Gly Gln Lys Leu Arg Ala Tyr Asn Leu

245 250 255

Gln Asp Asp Gly His Asp Thr Val Gln Ala Asn Val Glu Leu Gly Leu

260 265 270

Ala Val Asp Ser Arg Glu Tyr Gly Ile Gly Ala Gln Ile Leu Arg Asp

275 280 285

Met Gly Val Arg Thr Met Arg Leu Met Thr Asn Asn Pro Ala Lys Phe

290 295 300

Val Gly Leu Lys Gly Tyr Gly Leu Ala Val Val Gly Arg Val Pro Val

305 310 315 320

Ile Ser Pro Ile Thr Lys Glu Asn Gln Arg Tyr Leu Glu Thr Lys Arg

325 330 335

Thr Lys Met Gly His Val Tyr Gly Ser Asp Leu Pro Gly Asn Val Pro

340 345 350

Glu Glu Phe Leu Asn Pro Asp Asp Ile Ala Gly Asp Gln Asp Glu Asp

355 360 365

Asp Thr His Asn

370

<210> SEQ ID NO 83

<211> LENGTH: 475

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (140)

<221> NAME/KEY: unsure

<222> LOCATION: (404)

<221> NAME/KEY: unsure

<222> LOCATION: (450)

<221> NAME/KEY: unsure

<222> LOCATION: (460)

<221> NAME/KEY: unsure

<222> LOCATION: (463)

<400> SEQUENCE: 83

gcaaggtgaa ccaggagacg cgcggtagat acatagtagc tgcaagtaca ttaattcgca 60

ctgaagatta ccaaaaccaa tggctgcgcg gtgcagcggc ggctgcgacg ggcgcgagga 120

cagcgccgtg gaggcctggn tgcagtggca gaaggtgagc gacttcctga tcggggcgtc 180

ctacatgtcc atcccgctgg aactgctgca cttcgccacc tgcgccgacc tcgcgccgct 240

gcggtgggtg ctcctccagt tcggcgcctt catcgtgctc tgggctcgtc caactcgtcg 300

catttcacta cgcccgccgg atcgcgccgc ttctgccggc agtatggtgg ccaacttgtg 360

actgtaatcg gtgctgaaat acacaagacc gacgagtggc attnagacta cctactgcgc 420

gtatgtacaa aagcaagttt agcggtcggn taaagacaan canttgtatg aaatg 475

<210> SEQ ID NO 84

<211> LENGTH: 67

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (21)

<400> SEQUENCE: 84

Met Ala Ala Arg Cys Ser Gly Gly Cys Asp Gly Arg Glu Asp Ser Ala

1 5 10 15

Val Glu Ala Trp Xaa Gln Trp Gln Lys Val Ser Asp Phe Leu Ile Gly

20 25 30

Ala Ser Tyr Met Ser Ile Pro Leu Glu Leu Leu His Phe Ala Thr Cys

35 40 45

Ala Asp Leu Ala Pro Leu Arg Trp Val Leu Leu Gln Phe Gly Ala Phe

50 55 60

Ile Val Leu

65

<210> SEQ ID NO 85

<211> LENGTH: 2463

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 85

ccacgcgtcc gcgtctggtg aatgcaggag cgtcggcacc cgcagcgtgg aatcgacgac 60

gggcgcctcc agtcggttca gaaatgcgca aatgcgcgtc tgaatgaagc ctggttggag 120

gtggtagagc cgatggtggt gggaacggca ctgctgcgcg gggtttcctc cgcgtggatc 180

ctcctgttcc tctcctccct gctcctctcg ccgtcagcgg cgtctgtcga tttcggccac 240

tgcggcggct gcgacgacgc cgacgacggc gccctctcca gcacctataa catcctgcaa 300

tgccagaagg tcagcgactt cctcatcgcc gcggcctact tctccatccc gctcgagctg 360

ctctacttcg ccacctgctc cgacctcttc cccctcaaat ggatcgtgct gcagttcggc 420

gccttcatcg tgctctgcgg cctcacgcac ctcatcactg tgttcaccta cgagccgcac 480

tccttccacc tcgtactcgc ccttaccgtc gccaagttcc tgacggcact ggtctccttc 540

gcgacggcca tcaccctgct gacgctgata ccacagctcc tgagggtgaa ggtcagggaa 600

aacttcctga tgaacaaggc gcgtgagctg gaccgggagg tggggaggat gaaaaggaaa 660

gaagaggcga gctggcatgt gcgcatgctc acacaggaga tccgcaagtc gctcgacaga 720

cataccatct tgtacaccac catggttgag ctctcgaagg cactggaact gcagaattgt 780

gctgtctgga tgcctgatga gaccaggagc acgatgatct taacacatca gctgagggaa 840

agggatataa tggacccaca gaaacactcg attcctattg atgatccgga tgttcaagaa 900

ataaaggcaa ccaaggatgc aaaagttctt ggcccagatt cggcgctagg ggtttctagc 960

cgaagcaagc atgaagcagg gcctgtggct gcaataagga tgccgatgtt aagggtgtca 1020

aatttcaaag gagggactcc ggaagtgatg cagacgagct atgctatctt ggttctggtt 1080

ttgcctaatg atggttcatt agggtggggt cgaagagagt tggagattgt tgaggtagtt 1140

gctgaccaag ttgcagtcgc tctgtcacat gctgcactcc tagaggagtc tcagctgatg 1200

cgagagaagc ttgccgagca gcatagggac ttgctgcagg caaaggatga agccatgagg 1260

gcaggggacg ctaggaattc cttccagact gcaatgtacg atggaatgcg aaggccaatg 1320

cactcaatcc ttggtctcgt ctcaatgatg caacaggaga gcatgaatcc agagcaaagg 1380

›SEQUENCE LISTING · 21 of 53

cttgtgatgg atgccattgc caagacaagc agtgttgcat ccacactgat gaacgatgtg 1440

atgcaaacat cgacaatgaa ctgtgagcac ttgtctttgg tcaggaggcc gttcaacctt 1500

cattccttca ttaaagaagt tgttggagtg gtcagatgtc taactggttg caagggtgtg 1560

gagtttgagt ttcaagtgga gaattctttg ccagaaagga tcattggtga tgagaagaga 1620

gtcttccata ttgtcctgca catggtaggc actctaacag accgatgtaa tgctggctgt 1680

atctcattat atgtaaatgt ccataatgag gttgaagata ggcataatca tgactggatg 1740

ctgcgaagag caaacttctc tgggggctat gtatgtgtga aatttgagat taggattaga 1800

aaatcaaagg gctatctgtt gagttcatca agcagtcaga taagtcaggg atccaaaccc 1860

aacaattctg agatggggct tagcttcaat atgtgcaaga agattgtgca gatgatgaat 1920

ggcaatattt ggtcagtatc agattctaaa agcatcggag aaactatcat gctagtcctc 1980

cagttccagt tggaacctgt gactccggtc tctggagcgt cctcagattt gtacagatca 2040

tccgcaattc ccaactttaa tgggctcaga gtcctccttg cggacagcga ctgcaccaac 2100

cgagctgtaa ctcacaggct cctagagaag cttggttgcc gagtcctttc ggtcgcttct 2160

ggcgtccaat gcatcagctc cttcgctgcg gagtcgtcct tccagctggt ggttcttgat 2220

cttgacatgc agacgatgga tggattcgaa gtagcccgcg cgatcaggaa gttcagtagc 2280

aatagttggc tgccgttgat tattgcccta gcagcaagaa tcgacgacaa catccgggat 2340

cgttgccaga ggtcaggagt aaatggcctg atccagaaac cggtcacatt agccgcgctg 2400

ggagatgaac tgtatagagt ccttcagaac aattaaaaga gcctgacggt tctcatttct 2460

ttc 2463

<210> SEQ ID NO 86

<211> LENGTH: 804

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 86

Met Gln Glu Arg Arg His Pro Gln Arg Gly Ile Asp Asp Gly Arg Leu

1 5 10 15

Gln Ser Val Gln Lys Cys Ala Asn Ala Arg Leu Asn Glu Ala Trp Leu

20 25 30

Glu Val Val Glu Pro Met Val Val Gly Thr Ala Leu Leu Arg Gly Val

35 40 45

Ser Ser Ala Trp Ile Leu Leu Phe Leu Ser Ser Leu Leu Leu Ser Pro

50 55 60

Ser Ala Ala Ser Val Asp Phe Gly His Cys Gly Gly Cys Asp Asp Ala

65 70 75 80

Asp Asp Gly Ala Leu Ser Ser Thr Tyr Asn Ile Leu Gln Cys Gln Lys

85 90 95

Val Ser Asp Phe Leu Ile Ala Ala Ala Tyr Phe Ser Ile Pro Leu Glu

100 105 110

Leu Leu Tyr Phe Ala Thr Cys Ser Asp Leu Phe Pro Leu Lys Trp Ile

115 120 125

Val Leu Gln Phe Gly Ala Phe Ile Val Leu Cys Gly Leu Thr His Leu

130 135 140

Ile Thr Val Phe Thr Tyr Glu Pro His Ser Phe His Leu Val Leu Ala

145 150 155 160

Leu Thr Val Ala Lys Phe Leu Thr Ala Leu Val Ser Phe Ala Thr Ala

165 170 175

Ile Thr Leu Leu Thr Leu Ile Pro Gln Leu Leu Arg Val Lys Val Arg

180 185 190

Glu Asn Phe Leu Met Asn Lys Ala Arg Glu Leu Asp Arg Glu Val Gly

195 200 205

Arg Met Lys Arg Lys Glu Glu Ala Ser Trp His Val Arg Met Leu Thr

210 215 220

Gln Glu Ile Arg Lys Ser Leu Asp Arg His Thr Ile Leu Tyr Thr Thr

225 230 235 240

Met Val Glu Leu Ser Lys Ala Leu Glu Leu Gln Asn Cys Ala Val Trp

245 250 255

Met Pro Asp Glu Thr Arg Ser Thr Met Ile Leu Thr His Gln Leu Arg

260 265 270

Glu Arg Asp Ile Met Asp Pro Gln Lys His Ser Ile Pro Ile Asp Asp

275 280 285

Pro Asp Val Gln Glu Ile Lys Ala Thr Lys Asp Ala Lys Val Leu Gly

290 295 300

Pro Asp Ser Ala Leu Gly Val Ser Ser Arg Ser Lys His Glu Ala Gly

305 310 315 320

Pro Val Ala Ala Ile Arg Met Pro Met Leu Arg Val Ser Asn Phe Lys

325 330 335

Gly Gly Thr Pro Glu Val Met Gln Thr Ser Tyr Ala Ile Leu Val Leu

340 345 350

Val Leu Pro Asn Asp Gly Ser Leu Gly Trp Gly Arg Arg Glu Leu Glu

355 360 365

Ile Val Glu Val Val Ala Asp Gln Val Ala Val Ala Leu Ser His Ala

370 375 380

Ala Leu Leu Glu Glu Ser Gln Leu Met Arg Glu Lys Leu Ala Glu Gln

385 390 395 400

His Arg Asp Leu Leu Gln Ala Lys Asp Glu Ala Met Arg Ala Gly Asp

405 410 415

Ala Arg Asn Ser Phe Gln Thr Ala Met Tyr Asp Gly Met Arg Arg Pro

420 425 430

Met His Ser Ile Leu Gly Leu Val Ser Met Met Gln Gln Glu Ser Met

435 440 445

Asn Pro Glu Gln Arg Leu Val Met Asp Ala Ile Ala Lys Thr Ser Ser

450 455 460

Val Ala Ser Thr Leu Met Asn Asp Val Met Gln Thr Ser Thr Met Asn

465 470 475 480

Cys Glu His Leu Ser Leu Val Arg Arg Pro Phe Asn Leu His Ser Phe

485 490 495

Ile Lys Glu Val Val Gly Val Val Arg Cys Leu Thr Gly Cys Lys Gly

500 505 510

Val Glu Phe Glu Phe Gln Val Glu Asn Ser Leu Pro Glu Arg Ile Ile

515 520 525

Gly Asp Glu Lys Arg Val Phe His Ile Val Leu His Met Val Gly Thr

530 535 540

Leu Thr Asp Arg Cys Asn Ala Gly Cys Ile Ser Leu Tyr Val Asn Val

545 550 555 560

His Asn Glu Val Glu Asp Arg His Asn His Asp Trp Met Leu Arg Arg

565 570 575

Ala Asn Phe Ser Gly Gly Tyr Val Cys Val Lys Phe Glu Ile Arg Ile

580 585 590

Arg Lys Ser Lys Gly Tyr Leu Leu Ser Ser Ser Ser Ser Gln Ile Ser

595 600 605

Gln Gly Ser Lys Pro Asn Asn Ser Glu Met Gly Leu Ser Phe Asn Met

610 615 620

Cys Lys Lys Ile Val Gln Met Met Asn Gly Asn Ile Trp Ser Val Ser

625 630 635 640

Asp Ser Lys Ser Ile Gly Glu Thr Ile Met Leu Val Leu Gln Phe Gln

645 650 655

Leu Glu Pro Val Thr Pro Val Ser Gly Ala Ser Ser Asp Leu Tyr Arg

660 665 670

Ser Ser Ala Ile Pro Asn Phe Asn Gly Leu Arg Val Leu Leu Ala Asp

675 680 685

Ser Asp Cys Thr Asn Arg Ala Val Thr His Arg Leu Leu Glu Lys Leu

690 695 700

Gly Cys Arg Val Leu Ser Val Ala Ser Gly Val Gln Cys Ile Ser Ser

705 710 715 720

Phe Ala Ala Glu Ser Ser Phe Gln Leu Val Val Leu Asp Leu Asp Met

725 730 735

Gln Thr Met Asp Gly Phe Glu Val Ala Arg Ala Ile Arg Lys Phe Ser

740 745 750

Ser Asn Ser Trp Leu Pro Leu Ile Ile Ala Leu Ala Ala Arg Ile Asp

755 760 765

Asp Asn Ile Arg Asp Arg Cys Gln Arg Ser Gly Val Asn Gly Leu Ile

770 775 780

Gln Lys Pro Val Thr Leu Ala Ala Leu Gly Asp Glu Leu Tyr Arg Val

785 790 795 800

Leu Gln Asn Asn

<210> SEQ ID NO 87

<211> LENGTH: 2308

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 87

ccacgcgtcc gctttctccc gttcaccccg cgtgcacgct ctttcccttc ctcgtgccga 60

cgaccgggcg accgccgcgc cccggcccgc gcccccttgt ctcgggccac tcgccggcaa 120

cgagcagcca ccaggaaatg ttgtgaggac tgatgcaata actaagcttg ctggatggac 180

ggatgtgatt gcatcgagcc actatggcct accgatgatc tccttgtcaa gtatcagtac 240

atctcagact tcttcatagc cctagcgtac ttctctattc cgttggagct catatatttc 300

gtgaagaagt cgtccttctt cccgtacaga tgggtcttga tccagtttgg tgcgtttata 360

gttctctgtg gggcaaccca tctgataaac ctgtggacgt tcaccacaca tacaaagacc 420

gttgcgatgg tcatgaccat agcaaaggtt tctacagcag ttgtgtcctg tgcaactgct 480

ttgatgcttg ttcatatcat ccccgactta ttgagcgtga aaactagaga gttgttcctg 540

aagaataaag ctgaagagct tgacagagag atgggactga taaggacgca ggaggagacc 600

ggtagacatg ttaggatgct tacacatgaa atcagaagta ctcttgacag gcatacgatt 660

ttgaagacta ctcttgttga gctaggaagg accttgggtc tggaggaatg tgcattgtgg 720

›SEQUENCE LISTING · 22 of 53

atgccatctc gaagtggttc aagccttcag ctttctcata ctttgcacca ccagattact 780

gttggatcat cggtgccaat taatcttcct gtcatcaatc aagtgttcag tagcaaccgg 840

gcaattataa taccccacac atctcctttg gcgcggattc gacctcttac agggcgatat 900

gttccaccag aagtggctgc agtccgtgta cctcttctcc acctttcaaa cttccaaata 960

aatgattggc ctgagctttc agcaaaaagc tttgcaatca tggttttgat gcttccatct 1020

gatagtgcaa gaaaatggca tgtacatgaa ttggagctgg ttgaggtcgt tgctgatcag 1080

gtagcagttg cactatctca tgcggctatt cttgaagagt ccatgcgagc acgtgattta 1140

ctaatggagc agaatgttgc cctggattta gctcgaagag aggctgagat ggctatccgt 1200

gctcgcaatg atttcctagc tgttatgaat cacgaaatga gaacacccat gaatgcaata 1260

atagcccttt cctccttgct tttggaaact gaacttactc ctgagcagcg tctaatggtg 1320

gaaacagtac tgaaaagcag caatctgtta gcaacactca tcaatgatgt gctagatctt 1380

tccaaactcg aggatggaag ccttgaactg gagattaaag cattcaatct tcatgctgtt 1440

ttcaaagaag tgatgggttt cattaaacca attgcatcta tcaagaggct atctgtatcg 1500

gttatgttgg caccagattt gccgttatgt gccattggtg atgaaaagag actcatgcaa 1560

actattctga acatctctgg caacgctgta aagtttacca aggagggaca catcacactt 1620

gtagcttcca ttgtgaaggc tgactctttg agagagttca gaaccccaga atttcatcca 1680

actgcaagtg atgaccattt ctatttgaaa gttcaggtaa aagatacagg ctgtggaatt 1740

ggtccacagg atctacctca tgtatttaca aagtttgctc atcctcaaag cggaggaaac 1800

cgagggttta atggtagtgg tcttggcctt gccatatgca agaggtttgt tagtctcatg 1860

ggagggcaca tctggattga cagcgaagga accggaagag gttgcaccgc aacattcgtc 1920

gtcaagctcg gcgtgtgtga caacacaaac acctaccagc agcagctgat ccctctagta 1980

tggccaagca gtgcagactc cgatttgcgt gctccgaaag cgcttccgga cgggagagga 2040

tctacaccct tgaaatcccg gtaccaaagg agcgtatgag cctagtgtaa atgattgacg 2100

gcatagtgcc aagtagggga ccgattagtg ccaccgtcta attttgtttg taaccctgtc 2160

atagcaggca tatgatgtac aaatactgta aagcaaatgg agactgcggc cgtgtatctg 2220

ggtggcaacg ctgacttgct gcattgagtg gtatatacat atgctaccag cggattgaat 2280

tgcttaaaaa aaaaaaaaaa aaaaaaag 2308

<210> SEQ ID NO 88

<211> LENGTH: 634

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 88

Met Asp Gly Cys Asp Cys Ile Glu Pro Leu Trp Pro Thr Asp Asp Leu

1 5 10 15

Leu Val Lys Tyr Gln Tyr Ile Ser Asp Phe Phe Ile Ala Leu Ala Tyr

20 25 30

Phe Ser Ile Pro Leu Glu Leu Ile Tyr Phe Val Lys Lys Ser Ser Phe

35 40 45

Phe Pro Tyr Arg Trp Val Leu Ile Gln Phe Gly Ala Phe Ile Val Leu

50 55 60

Cys Gly Ala Thr His Leu Ile Asn Leu Trp Thr Phe Thr Thr His Thr

65 70 75 80

Lys Thr Val Ala Met Val Met Thr Ile Ala Lys Val Ser Thr Ala Val

85 90 95

Val Ser Cys Ala Thr Ala Leu Met Leu Val His Ile Ile Pro Asp Leu

100 105 110

Leu Ser Val Lys Thr Arg Glu Leu Phe Leu Lys Asn Lys Ala Glu Glu

115 120 125

Leu Asp Arg Glu Met Gly Leu Ile Arg Thr Gln Glu Glu Thr Gly Arg

130 135 140

His Val Arg Met Leu Thr His Glu Ile Arg Ser Thr Leu Asp Arg His

145 150 155 160

Thr Ile Leu Lys Thr Thr Leu Val Glu Leu Gly Arg Thr Leu Gly Leu

165 170 175

Glu Glu Cys Ala Leu Trp Met Pro Ser Arg Ser Gly Ser Ser Leu Gln

180 185 190

Leu Ser His Thr Leu His His Gln Ile Thr Val Gly Ser Ser Val Pro

195 200 205

Ile Asn Leu Pro Val Ile Asn Gln Val Phe Ser Ser Asn Arg Ala Ile

210 215 220

Ile Ile Pro His Thr Ser Pro Leu Ala Arg Ile Arg Pro Leu Thr Gly

225 230 235 240

Arg Tyr Val Pro Pro Glu Val Ala Ala Val Arg Val Pro Leu Leu His

245 250 255

Leu Ser Asn Phe Gln Ile Asn Asp Trp Pro Glu Leu Ser Ala Lys Ser

260 265 270

Phe Ala Ile Met Val Leu Met Leu Pro Ser Asp Ser Ala Arg Lys Trp

275 280 285

His Val His Glu Leu Glu Leu Val Glu Val Val Ala Asp Gln Val Ala

290 295 300

Val Ala Leu Ser His Ala Ala Ile Leu Glu Glu Ser Met Arg Ala Arg

305 310 315 320

Asp Leu Leu Met Glu Gln Asn Val Ala Leu Asp Leu Ala Arg Arg Glu

325 330 335

Ala Glu Met Ala Ile Arg Ala Arg Asn Asp Phe Leu Ala Val Met Asn

340 345 350

His Glu Met Arg Thr Pro Met Asn Ala Ile Ile Ala Leu Ser Ser Leu

355 360 365

Leu Leu Glu Thr Glu Leu Thr Pro Glu Gln Arg Leu Met Val Glu Thr

370 375 380

Val Leu Lys Ser Ser Asn Leu Leu Ala Thr Leu Ile Asn Asp Val Leu

385 390 395 400

Asp Leu Ser Lys Leu Glu Asp Gly Ser Leu Glu Leu Glu Ile Lys Ala

405 410 415

Phe Asn Leu His Ala Val Phe Lys Glu Val Met Gly Phe Ile Lys Pro

420 425 430

Ile Ala Ser Ile Lys Arg Leu Ser Val Ser Val Met Leu Ala Pro Asp

435 440 445

Leu Pro Leu Cys Ala Ile Gly Asp Glu Lys Arg Leu Met Gln Thr Ile

450 455 460

Leu Asn Ile Ser Gly Asn Ala Val Lys Phe Thr Lys Glu Gly His Ile

465 470 475 480

Thr Leu Val Ala Ser Ile Val Lys Ala Asp Ser Leu Arg Glu Phe Arg

485 490 495

Thr Pro Glu Phe His Pro Thr Ala Ser Asp Asp His Phe Tyr Leu Lys

500 505 510

Val Gln Val Lys Asp Thr Gly Cys Gly Ile Gly Pro Gln Asp Leu Pro

515 520 525

His Val Phe Thr Lys Phe Ala His Pro Gln Ser Gly Gly Asn Arg Gly

530 535 540

Phe Asn Gly Ser Gly Leu Gly Leu Ala Ile Cys Lys Arg Phe Val Ser

545 550 555 560

Leu Met Gly Gly His Ile Trp Ile Asp Ser Glu Gly Thr Gly Arg Gly

565 570 575

Cys Thr Ala Thr Phe Val Val Lys Leu Gly Val Cys Asp Asn Thr Asn

580 585 590

Thr Tyr Gln Gln Gln Leu Ile Pro Leu Val Trp Pro Ser Ser Ala Asp

595 600 605

Ser Asp Leu Arg Ala Pro Lys Ala Leu Pro Asp Gly Arg Gly Ser Thr

610 615 620

Pro Leu Lys Ser Arg Tyr Gln Arg Ser Val

625 630

<210> SEQ ID NO 89

<211> LENGTH: 2593

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 89

gcacgagctt acaattgcat tgagccgctc tggcaggcgg atgatctcct tgtaaagtat 60

cagtacatat ctgacttctt tattgcgctc gcttacttct cgatcccttt ggagctcata 120

tatttcgtta agaagtcagc attcttccct taccgatggg tgcttataca attcggcgca 180

ttcattgttc tttgtggggc aacccacctg ataaatttgt ggacttttgc catatatacc 240

aagactatag ctgtggtact gacagtggcg aaagcagcga cagcggttgt ttcgtgcatc 300

acagctttga tgcttgtgca tataattcct gatttgttga atgtgaagtt gagagagaga 360

tttctgaagg ataaggctga tgagcttgat agagagatgg ggattataag aacacaagag 420

gagacaggaa gacatgtcca catgctgacc catgagataa gaagcacact tgacaggcac 480

accattctgc gaactacgct cgtcgagctg ggaaggactc ttgttttagc ggagtgtgcc 540

ctgtggatgc caacacgctc tggatcggcc cttcagctct ctcatacgat atataacagc 600

gcagcaattg gatcagttgt tcctatcaac cttcccattg tcagtaaggt ttttaatagt 660

aaccgtgtag taaaaattcc gcatacctcc ccgttagctt cgataacggc tgacaaaagc 720

agacagtgca agagaatggc ggccgcatga acgggagctt gttgaagtcg ttgctgatca 780

ggtagcggtc gcattgtctc atgctgccat tttggaagag tccatgcggg cccgtgatct 840

actaatggag caaaacattg ctcttgatgc agcacgtcgg gaggcagaaa tggctatttg 900

›SEQUENCE LISTING · 23 of 53

tgcccgtaat gattttcttg ctgtaatgaa ccatgaaatg cggactccta tgcgagcaat 960

cgtttctttg tcctctctcc ttttagaaac aaatcttagt gctgaacaac gcctaatggt 1020

tgagactata ctaaagagta gcgatcttct ggcaactctt acgaatgatg ttttggacgt 1080

ttcaaagctt gagaatggga gtcttgagct ggaaattgca ccttttaatt tgcattccac 1140

ctttacagat gtggttaatt tgattaagcc agtagcagcg tgcaagaggc tctcggttat 1200

ggtcactttg gcaccagagt tacctctaca tgctattggt gatcaaaagc gattgatgca 1260

aataatccta aatgttgccg ggaactccat taagttcaca aaggagggtc atgtttcgat 1320

tacagcttct atggccagac cagatgcttt gagaggtcca catgaacctg actaccatcc 1380

agttgtctct gatggattct tttacctggc tgttcaggta aaagacacag gctgcggaat 1440

cagccctcag gatatgcccc acacattcag aaagtttgca caccctgaaa acgcaggcaa 1500

atggaacagt ggcagtggat tggggctggc cctttccaga agatttgtca gtctaatgga 1560

aggtaacatc tggctcgaga gcgaaggcgt cgggaagggc tgtaccgcga tgttctttgt 1620

gaaacttggc atgcctgaga aaccaaatgc aaacctccga agaatggcgc cgcaccctct 1680

acagccaaac caaggagctg gaggccctga tgccctcagt atatccataa tggacagcaa 1740

cccgagggtt cctcgggttc gctaccaatc aagcgtatga gaagcaccga tgatgccatc 1800

gccatgccaa ggcagagcat ccgacccagt tttgccatca cagtttacag ctccatttgc 1860

agctctcttt ttgatgtatg gatggtagat gttgttcttg tccatcagtg gaggtacctt 1920

gggcatgctg gtataagttt cattggcatt cttgaatctt gatttcagtt gtgagggtga 1980

ttgggtggtc ttaagtggtg acaattgtga aagctgatgt agtgatcttt tgcagctctc 2040

tttttgatgt atggatggta gatgttgttc ttgtccatca gtagaggtac cttttgggca 2100

tgatggtata agtttcattg gcattcttga ttgcagttgt gagggcgatt gggtgatctt 2160

aagtggtgac aattgggaaa gctgatgtag tgatctgttg tctgcacgag tctgaagtta 2220

ctgggaagtg ccaatttact gcaagtgaca gtgaacatga aggtctatgg ccatcttaca 2280

tctctgatac cctgagctat agaagagctt ggacgaacgt gtttattttt cttacttttt 2340

tttccctaga ttagggctac aacctggaag ggatcgatct actagctgat tgcagtatca 2400

tttgtacttt ttgttttttt tttctgaaat aagagtatca gtggtaattg taagcgttac 2460

tgcagtgctt tataagcaca agcaaattat catcaaatca aattatagaa aaatctagtg 2520

aattaaaaag agctcaataa gatgaaccta caagcgaagt gtttactgct caaaaaaaaa 2580

aaaaaaaaaa aaa 2593

<210> SEQ ID NO 90

<211> LENGTH: 592

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (236)..(237)..(238)..(239)

<400> SEQUENCE: 90

Ala Arg Ala Tyr Asn Cys Ile Glu Pro Leu Trp Gln Ala Asp Asp Leu

1 5 10 15

Leu Val Lys Tyr Gln Tyr Ile Ser Asp Phe Phe Ile Ala Leu Ala Tyr

20 25 30

Phe Ser Ile Pro Leu Glu Leu Ile Tyr Phe Val Lys Lys Ser Ala Phe

35 40 45

Phe Pro Tyr Arg Trp Val Leu Ile Gln Phe Gly Ala Phe Ile Val Leu

50 55 60

Cys Gly Ala Thr His Leu Ile Asn Leu Trp Thr Phe Ala Ile Tyr Thr

65 70 75 80

Lys Thr Ile Ala Val Val Leu Thr Val Ala Lys Ala Ala Thr Ala Val

85 90 95

Val Ser Cys Ile Thr Ala Leu Met Leu Val His Ile Ile Pro Asp Leu

100 105 110

Leu Asn Val Lys Leu Arg Glu Arg Phe Leu Lys Asp Lys Ala Asp Glu

115 120 125

Leu Asp Arg Glu Met Gly Ile Ile Arg Thr Gln Glu Glu Thr Gly Arg

130 135 140

His Val His Met Leu Thr His Glu Ile Arg Ser Thr Leu Asp Arg His

145 150 155 160

Thr Ile Leu Arg Thr Thr Leu Val Glu Leu Gly Arg Thr Leu Val Leu

165 170 175

Ala Glu Cys Ala Leu Trp Met Pro Thr Arg Ser Gly Ser Ala Leu Gln

180 185 190

Leu Ser His Thr Ile Tyr Asn Ser Ala Ala Ile Gly Ser Val Val Pro

195 200 205

Ile Asn Leu Pro Ile Val Ser Lys Val Phe Asn Ser Asn Arg Val Val

210 215 220

Lys Ile Pro His Thr Ser Pro Leu Ala Ser Ile Xaa Xaa Xaa Xaa Ala

225 230 235 240

Asp Ser Ala Arg Glu Trp Arg Pro His Glu Arg Glu Leu Val Glu Val

245 250 255

Val Ala Asp Gln Val Ala Val Ala Leu Ser His Ala Ala Ile Leu Glu

260 265 270

Glu Ser Met Arg Ala Arg Asp Leu Leu Met Glu Gln Asn Ile Ala Leu

275 280 285

Asp Ala Ala Arg Arg Glu Ala Glu Met Ala Ile Cys Ala Arg Asn Asp

290 295 300

Phe Leu Ala Val Met Asn His Glu Met Arg Thr Pro Met Arg Ala Ile

305 310 315 320

Val Ser Leu Ser Ser Leu Leu Leu Glu Thr Asn Leu Ser Ala Glu Gln

325 330 335

Arg Leu Met Val Glu Thr Ile Leu Lys Ser Ser Asp Leu Leu Ala Thr

340 345 350

Leu Thr Asn Asp Val Leu Asp Val Ser Lys Leu Glu Asn Gly Ser Leu

355 360 365

Glu Leu Glu Ile Ala Pro Phe Asn Leu His Ser Thr Phe Thr Asp Val

370 375 380

Val Asn Leu Ile Lys Pro Val Ala Ala Cys Lys Arg Leu Ser Val Met

385 390 395 400

Val Thr Leu Ala Pro Glu Leu Pro Leu His Ala Ile Gly Asp Gln Lys

405 410 415

Arg Leu Met Gln Ile Ile Leu Asn Val Ala Gly Asn Ser Ile Lys Phe

420 425 430

Thr Lys Glu Gly His Val Ser Ile Thr Ala Ser Met Ala Arg Pro Asp

435 440 445

Ala Leu Arg Gly Pro His Glu Pro Asp Tyr His Pro Val Val Ser Asp

450 455 460

Gly Phe Phe Tyr Leu Ala Val Gln Val Lys Asp Thr Gly Cys Gly Ile

465 470 475 480

Ser Pro Gln Asp Met Pro His Thr Phe Arg Lys Phe Ala His Pro Glu

485 490 495

Asn Ala Gly Lys Trp Asn Ser Gly Ser Gly Leu Gly Leu Ala Leu Ser

500 505 510

Arg Arg Phe Val Ser Leu Met Glu Gly Asn Ile Trp Leu Glu Ser Glu

515 520 525

Gly Val Gly Lys Gly Cys Thr Ala Met Phe Phe Val Lys Leu Gly Met

530 535 540

Pro Glu Lys Pro Asn Ala Asn Leu Arg Arg Met Ala Pro His Pro Leu

545 550 555 560

Gln Pro Asn Gln Gly Ala Gly Gly Pro Asp Ala Leu Ser Ile Ser Ile

565 570 575

Met Asp Ser Asn Pro Arg Val Pro Arg Val Arg Tyr Gln Ser Ser Val

580 585 590

<210> SEQ ID NO 91

<211> LENGTH: 2311

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 91

gcacgagtgt cttcacctaa ccaaatgggc gcctccacca cctgatccac ctctgtttct 60

tcttcctttc atccgttcat acgggggtta gatgatggag tcctgtgatt gtatagatac 120

gcagtatccg ccggatgaac ttcttgtgaa gtatcagtat atctcggatg tgctaattgc 180

tcttgcgtat ttttcgattc cggtggagct catctatttt gttcagaagt ctgctttctt 240

tccgtataga tgggtgctta tgcagtttgg tgcctttatt gttctctgtg gagcaactca 300

tttcataaac ctgtggacat tctctccgca ctctaagtct gttgctgttg tcatgacgat 360

tgccaaagtt tcgtgtgcta ttgtgtcatg tgcgacggca ctgatgcttg tacacattat 420

tcccgatctg ttgagtgtca agaggcgcga atcgatcctc aagaacaagg ctgaagagct 480

tgacagagag atgggactta ttcttactca ggaagagacg ggaagacatg ttagaatgtt 540

gactcatgaa attaggagca cacttgacag gcatacaatt ttaaagacta ctcttgtgga 600

gctggggagg actttgggct tggaggagtg tgcattatgg atgccttcga gaagtggtct 660

gaatctgcaa ctttcccata ctttaaccta tcatgtgcaa gttgggtcta cggtgcaaac 720

aaacaatcct attgtcaatg aagttttcaa cagccctcaa gctatgcgga taccacccac 780

ctgtccattg gccaggatca gacctcttgt gggaagatat gtgccccctg aagttgttgc 840

tgttcgggtg ccgcttttga atctgtcaaa ttttcaaatt aacgattggc ctgatatttc 900

›SEQUENCE LISTING · 24 of 53

agcaaagagc tatgcaatca tggttctcat cctccctact gacagtgtta gaaaatggcg 960

agaccatgag ttggaacttg ttgatgttgt tgcagatcag gtagcagttg ccctttcaca 1020

tgctgctatt ttggaggagt ctatgcgtgc ccgtgatcaa ctcatggagc agaatgttgc 1080

tttagattta gcccggcgag aggcagagat ggcaattcat gctcgcaatg attttcttgc 1140

tgtcatgaat catgaaatga ggacaccaat gcatgcaatt atagcattgt catcacttct 1200

cttggagact gaactgacgc cagagcaaag ggttatgata gagacagtgt tgaagagtag 1260

taatgttttg gcaacactca ttaatgatgt tctagatctt tctcgacttg aagatggtag 1320

cctcgaatta gaaatgggaa aattcaacct ccatggagtt ttgggagaga ttgttgaact 1380

gataaaacca atagcatccg tgaaaaagtt acctatcacc ttaattctgt ctcctgatct 1440

gcctactcat gccattggtg atgaaaagcg gcttacacaa actcttttga atgtcgtggg 1500

taatgctgtc aaattcacta aggagggcta tgtttctgta agagtatcag ttgcaaaacc 1560

agaatcttca caggattggc gacctccaga gttttatcca gcatctagtg atggccattt 1620

ctacattcga gtccaggtta aggactctgg atgtgggatt ctcccacaag atattccgca 1680

tctctttacc aagtttgccc agtctcggag tggaccagct cgacctagca gtggtgcagg 1740

tcttgggctt gccatttgta aaagatttgt aaacctcatg ggaggccaca tatggattga 1800

gagtgagggc cttgacaaag gaagcacagc tacatttata gtcaaacttg ggatttgtgg 1860

caatccagat ccatccgatc atcaagctgc aaacagaagc caagcatata gtggaagtgg 1920

tggcctcgct agatttaaac ccttcatcac agatgaaaac gacactggtt tttctactag 1980

acgcaatcaa agaagtttct aatatccaag acaccaaaat tgcatacctc tcaatcacag 2040

tcgacaagat ggagtaaacc tcctactttc attttgcatg tgctaaattg tataatttgc 2100

agtttgcggc tgtagaatag gattactatg tcaggcatat ttctaacaaa caggttttat 2160

gcgaactcct gttgtattaa ctggaataca atacaacaaa acaaagttga gagacgatct 2220

gttacttaat ttgacttgta gtatatattg tcagtggcaa aaaaaaaaaa aaaaaaaaaa 2280

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a 2311

<210> SEQ ID NO 92

<211> LENGTH: 636

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 92

Met Met Glu Ser Cys Asp Cys Ile Asp Thr Gln Tyr Pro Pro Asp Glu

1 5 10 15

Leu Leu Val Lys Tyr Gln Tyr Ile Ser Asp Val Leu Ile Ala Leu Ala

20 25 30

Tyr Phe Ser Ile Pro Val Glu Leu Ile Tyr Phe Val Gln Lys Ser Ala

35 40 45

Phe Phe Pro Tyr Arg Trp Val Leu Met Gln Phe Gly Ala Phe Ile Val

50 55 60

Leu Cys Gly Ala Thr His Phe Ile Asn Leu Trp Thr Phe Ser Pro His

65 70 75 80

Ser Lys Ser Val Ala Val Val Met Thr Ile Ala Lys Val Ser Cys Ala

85 90 95

Ile Val Ser Cys Ala Thr Ala Leu Met Leu Val His Ile Ile Pro Asp

100 105 110

Leu Leu Ser Val Lys Arg Arg Glu Ser Ile Leu Lys Asn Lys Ala Glu

115 120 125

Glu Leu Asp Arg Glu Met Gly Leu Ile Leu Thr Gln Glu Glu Thr Gly

130 135 140

Arg His Val Arg Met Leu Thr His Glu Ile Arg Ser Thr Leu Asp Arg

145 150 155 160

His Thr Ile Leu Lys Thr Thr Leu Val Glu Leu Gly Arg Thr Leu Gly

165 170 175

Leu Glu Glu Cys Ala Leu Trp Met Pro Ser Arg Ser Gly Leu Asn Leu

180 185 190

Gln Leu Ser His Thr Leu Thr Tyr His Val Gln Val Gly Ser Thr Val

195 200 205

Gln Thr Asn Asn Pro Ile Val Asn Glu Val Phe Asn Ser Pro Gln Ala

210 215 220

Met Arg Ile Pro Pro Thr Cys Pro Leu Ala Arg Ile Arg Pro Leu Val

225 230 235 240

Gly Arg Tyr Val Pro Pro Glu Val Val Ala Val Arg Val Pro Leu Leu

245 250 255

Asn Leu Ser Asn Phe Gln Ile Asn Asp Trp Pro Asp Ile Ser Ala Lys

260 265 270

Ser Tyr Ala Ile Met Val Leu Ile Leu Pro Thr Asp Ser Val Arg Lys

275 280 285

Trp Arg Asp His Glu Leu Glu Leu Val Asp Val Val Ala Asp Gln Val

290 295 300

Ala Val Ala Leu Ser His Ala Ala Ile Leu Glu Glu Ser Met Arg Ala

305 310 315 320

Arg Asp Gln Leu Met Glu Gln Asn Val Ala Leu Asp Leu Ala Arg Arg

325 330 335

Glu Ala Glu Met Ala Ile His Ala Arg Asn Asp Phe Leu Ala Val Met

340 345 350

Asn His Glu Met Arg Thr Pro Met His Ala Ile Ile Ala Leu Ser Ser

355 360 365

Leu Leu Leu Glu Thr Glu Leu Thr Pro Glu Gln Arg Val Met Ile Glu

370 375 380

Thr Val Leu Lys Ser Ser Asn Val Leu Ala Thr Leu Ile Asn Asp Val

385 390 395 400

Leu Asp Leu Ser Arg Leu Glu Asp Gly Ser Leu Glu Leu Glu Met Gly

405 410 415

Lys Phe Asn Leu His Gly Val Leu Gly Glu Ile Val Glu Leu Ile Lys

420 425 430

Pro Ile Ala Ser Val Lys Lys Leu Pro Ile Thr Leu Ile Leu Ser Pro

435 440 445

Asp Leu Pro Thr His Ala Ile Gly Asp Glu Lys Arg Leu Thr Gln Thr

450 455 460

Leu Leu Asn Val Val Gly Asn Ala Val Lys Phe Thr Lys Glu Gly Tyr

465 470 475 480

Val Ser Val Arg Val Ser Val Ala Lys Pro Glu Ser Ser Gln Asp Trp

485 490 495

Arg Pro Pro Glu Phe Tyr Pro Ala Ser Ser Asp Gly His Phe Tyr Ile

500 505 510

Arg Val Gln Val Lys Asp Ser Gly Cys Gly Ile Leu Pro Gln Asp Ile

515 520 525

Pro His Leu Phe Thr Lys Phe Ala Gln Ser Arg Ser Gly Pro Ala Arg

530 535 540

Pro Ser Ser Gly Ala Gly Leu Gly Leu Ala Ile Cys Lys Arg Phe Val

545 550 555 560

Asn Leu Met Gly Gly His Ile Trp Ile Glu Ser Glu Gly Leu Asp Lys

565 570 575

Gly Ser Thr Ala Thr Phe Ile Val Lys Leu Gly Ile Cys Gly Asn Pro

580 585 590

Asp Pro Ser Asp His Gln Ala Ala Asn Arg Ser Gln Ala Tyr Ser Gly

595 600 605

Ser Gly Gly Leu Ala Arg Phe Lys Pro Phe Ile Thr Asp Glu Asn Asp

610 615 620

Thr Gly Phe Ser Thr Arg Arg Asn Gln Arg Ser Phe

625 630 635

<210> SEQ ID NO 93

<211> LENGTH: 756

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 93

tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttgtt 60

gttggttatg gtgctttctg tttatgccaa cgatgtggaa tattcacagt gcaattgtga 120

tgaggaaggg ctttggagca tccacagcgt gctggtttgt cagaaagtga gtgacttttt 180

cattgccatt gcatattttt ccatacccct tgagcttctt tactttgtga gctgctccaa 240

tgtccccttt aaattggtct tccttcaatt cattgccttc atagttctct gtggattgac 300

ccatttgctc aatgcctata cttactatgg ccctcactcc ttccagttgt ttctttccct 360

tactgttgct aagttcctca ctgctcttgt ttcttgtgcc actgcaattt cctttcccac 420

actcattcct cttttgctga aaatcaaagt gagggagcta tttttgaggc agaatgtgtt 480

ggaattgggc caagaggttg ggatgatgaa gaaacagaag gaagctagtt ggcatgttag 540

gatgctcact tgtgagatta ggaaatctct ggataagcat accatcttgt ataccacact 600

tgttgagctt tccaaggcat tggatttgca caattgtgca gtatggatgc ctgatgagga 660

taggagagaa atgcatttga ctcatgagtt gaaaccaagc tcagcgagga gttttcacaa 720

ttctattgct atcagtgatc ctgatgtgtc tcgtgc 756

<210> SEQ ID NO 94

<211> LENGTH: 233

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 94

Leu Leu Leu Val Met Val Leu Ser Val Tyr Ala Asn Asp Val Glu Tyr

1 5 10 15

Ser Gln Cys Asn Cys Asp Glu Glu Gly Leu Trp Ser Ile His Ser Val

20 25 30

Leu Val Cys Gln Lys Val Ser Asp Phe Phe Ile Ala Ile Ala Tyr Phe

35 40 45

Ser Ile Pro Leu Glu Leu Leu Tyr Phe Val Ser Cys Ser Asn Val Pro

›SEQUENCE LISTING · 25 of 53

50 55 60

Phe Lys Leu Val Phe Leu Gln Phe Ile Ala Phe Ile Val Leu Cys Gly

65 70 75 80

Leu Thr His Leu Leu Asn Ala Tyr Thr Tyr Tyr Gly Pro His Ser Phe

85 90 95

Gln Leu Phe Leu Ser Leu Thr Val Ala Lys Phe Leu Thr Ala Leu Val

100 105 110

Ser Cys Ala Thr Ala Ile Ser Phe Pro Thr Leu Ile Pro Leu Leu Leu

115 120 125

Lys Ile Lys Val Arg Glu Leu Phe Leu Arg Gln Asn Val Leu Glu Leu

130 135 140

Gly Gln Glu Val Gly Met Met Lys Lys Gln Lys Glu Ala Ser Trp His

145 150 155 160

Val Arg Met Leu Thr Cys Glu Ile Arg Lys Ser Leu Asp Lys His Thr

165 170 175

Ile Leu Tyr Thr Thr Leu Val Glu Leu Ser Lys Ala Leu Asp Leu His

180 185 190

Asn Cys Ala Val Trp Met Pro Asp Glu Asp Arg Arg Glu Met His Leu

195 200 205

Thr His Glu Leu Lys Pro Ser Ser Ala Arg Ser Phe His Asn Ser Ile

210 215 220

Ala Ile Ser Asp Pro Asp Val Ser Arg

225 230

<210> SEQ ID NO 95

<211> LENGTH: 1603

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 95

gcacgaggtt cgtcgccctt ttgccaacct ctccttccct ccctccttcc tttaccccct 60

ctccatctag ctgccgtcgc catgccaccg ccctgccgca cccaacacca actccggtgc 120

tcgccccaca agcagggaac aagtacaggc caggaagaca agtaaacaac aacgacactc 180

tatagttagg catccaggct ccagattaac taaatggagg gctgtgcctg catcgagcag 240

ttccggcgaa ctgacgagct cctcatcaag taccagtaca tctcggactt cttcatagcc 300

ctcgcctact tctccatccc actcgagctg atctacttcg ccaagaagtc gggatccttc 360

ccctacagat gggtgctcct ccagttcagc gccttcattg tcctctgcgg ggccacccac 420

ctcataaacc tctggacctt caccaagcac acccagaccg tcgacatcgt catgacggta 480

accaaggtca ccacggccgt cgtttcctgc gcgacggccc tcacgctcat ccatatcata 540

cctgatttgt tgggtgtcaa gacgagggag ttgttcctca agaagaaggc cgacgagctc 600

gacagggaga tgagcctcat gcggtcgcag gaggacaccg ggaggcatgt caggatgctc 660

atccatgaga tcaggagcac gcttgacagg cacaccatcc tcaagactac tctggttgag 720

ctcgggagga cgctgggtct ggaggaatgt gctttctgga tgccgtcgag gagcggttcg 780

agccttgagc taactcatac catgcgccac cacatcccca cgggctcttc tgtggagatt 840

aatctccctg ttgtcaacca agtcttaagt accaaccgtg cgatcatagt gccacatact 900

tctcctttgg cgaggatcca ccctgttcaa gggcggcatg tcccacccga ggtggctgct 960

gtgagagttc ctctgctgca tctttcaaac tttcatcccg agcttttggc gaaaagctac 1020

gcaattatgg ttttgatgct cccatctgat agtgtgagga aatggcttgc tcatgaactc 1080

gagctcatcg aggtcgtcgc caatcaggta gctgttgccc tctctcaagc ggaaattctt 1140

gaagaatcaa tgcgagcacg tgatctactg acggagcaaa atgtcgccct ggatttagct 1200

ctgcgggagg ctgaaatggc aatacatgct cgcaatgtca acagggtggg ggttgaactt 1260

gtggggcctg aggacgcaga caacgtcgct accatcaact catctgcttc tgcagccaag 1320

gtgcacaacg ccgagccatg atcgacgcgt ctaccccaac ttcgcccgac actgagatgc 1380

gtcacttcta cccaccaaga agtacgcctt gatccaaatc gtactctcta gttgcctttg 1440

cttgcaccct ataggtgccc caaaggccca aaccgtttcc tttgctgctg ctgcgacagc 1500

actatctatc tgaacctatg tagtttaaat ttggccctgc tgtgtgcaat ctatctggat 1560

ttgtgctaag ttatgctatt tatctaaaaa aaaaaaaaaa aaa 1603

<210> SEQ ID NO 96

<211> LENGTH: 375

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 96

Met Glu Gly Cys Ala Cys Ile Glu Gln Phe Arg Arg Thr Asp Glu Leu

1 5 10 15

Leu Ile Lys Tyr Gln Tyr Ile Ser Asp Phe Phe Ile Ala Leu Ala Tyr

20 25 30

Phe Ser Ile Pro Leu Glu Leu Ile Tyr Phe Ala Lys Lys Ser Gly Ser

35 40 45

Phe Pro Tyr Arg Trp Val Leu Leu Gln Phe Ser Ala Phe Ile Val Leu

50 55 60

Cys Gly Ala Thr His Leu Ile Asn Leu Trp Thr Phe Thr Lys His Thr

65 70 75 80

Gln Thr Val Asp Ile Val Met Thr Val Thr Lys Val Thr Thr Ala Val

85 90 95

Val Ser Cys Ala Thr Ala Leu Thr Leu Ile His Ile Ile Pro Asp Leu

100 105 110

Leu Gly Val Lys Thr Arg Glu Leu Phe Leu Lys Lys Lys Ala Asp Glu

115 120 125

Leu Asp Arg Glu Met Ser Leu Met Arg Ser Gln Glu Asp Thr Gly Arg

130 135 140

His Val Arg Met Leu Ile His Glu Ile Arg Ser Thr Leu Asp Arg His

145 150 155 160

Thr Ile Leu Lys Thr Thr Leu Val Glu Leu Gly Arg Thr Leu Gly Leu

165 170 175

Glu Glu Cys Ala Phe Trp Met Pro Ser Arg Ser Gly Ser Ser Leu Glu

180 185 190

Leu Thr His Thr Met Arg His His Ile Pro Thr Gly Ser Ser Val Glu

195 200 205

Ile Asn Leu Pro Val Val Asn Gln Val Leu Ser Thr Asn Arg Ala Ile

210 215 220

Ile Val Pro His Thr Ser Pro Leu Ala Arg Ile His Pro Val Gln Gly

225 230 235 240

Arg His Val Pro Pro Glu Val Ala Ala Val Arg Val Pro Leu Leu His

245 250 255

Leu Ser Asn Phe His Pro Glu Leu Leu Ala Lys Ser Tyr Ala Ile Met

260 265 270

Val Leu Met Leu Pro Ser Asp Ser Val Arg Lys Trp Leu Ala His Glu

275 280 285

Leu Glu Leu Ile Glu Val Val Ala Asn Gln Val Ala Val Ala Leu Ser

290 295 300

Gln Ala Glu Ile Leu Glu Glu Ser Met Arg Ala Arg Asp Leu Leu Thr

305 310 315 320

Glu Gln Asn Val Ala Leu Asp Leu Ala Leu Arg Glu Ala Glu Met Ala

325 330 335

Ile His Ala Arg Asn Val Asn Arg Val Gly Val Glu Leu Val Gly Pro

340 345 350

Glu Asp Ala Asp Asn Val Ala Thr Ile Asn Ser Ser Ala Ser Ala Ala

355 360 365

Lys Val His Asn Ala Glu Pro

370 375

<210> SEQ ID NO 97

<211> LENGTH: 491

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (418)

<221> NAME/KEY: unsure

<222> LOCATION: (428)

<221> NAME/KEY: unsure

<222> LOCATION: (446)

<221> NAME/KEY: unsure

<222> LOCATION: (476)

<221> NAME/KEY: unsure

<222> LOCATION: (491)

<400> SEQUENCE: 97

gcgtctggtg aatgcaggag cgtcggcacc cgcagcgtgg aatcgacgac gggcgcctcc 60

agtcggttca gaaatgcgca aatgcgcgtc tgaatgaagc ctggttggag gtggtagagc 120

cgatggtggt gggaacggca ctgctgcgcg gggtttctcc gcgtggatcc tcctgttcct 180

ctcctccctg ctcctctcgc cgtcagcggc gtctgtcgat ttcggccact gcggcggctg 240

cgacgacgcc gacgacggcg ccctctccag cacctataac atcctgcaat gccagaaggt 300

cagcgacttc ctcatcgccg cggctacttc tccatcccgc tcgagctgtc tacttcgcca 360

cctgctccga cctcttcccc ctcaaatgga tcgtgctgca gttcggcgcc ttcatcgngc 420

tctgcggnct acgcacctca tactgngtca cctacgagcc gacttcttca acttgnactt 480

ggccttaccg n 491

<210> SEQ ID NO 98

<211> LENGTH: 89

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (53)

<221> NAME/KEY: UNSURE

<222> LOCATION: (62)

<221> NAME/KEY: UNSURE

<222> LOCATION: (70)

<221> NAME/KEY: UNSURE

<222> LOCATION: (85)

<400> SEQUENCE: 98

Ile Leu Leu Phe Leu Ser Ser Leu Leu Leu Ser Pro Ser Ala Ala Ser

1 5 10 15

Val Asp Phe Gly His Cys Gly Gly Cys Asp Asp Ala Asp Asp Gly Ala

20 25 30

Leu Ser Ser Thr Tyr Asn Ile Leu Gln Cys Gln Lys Val Ser Asp Phe

35 40 45

Leu Ile Ala Ala Xaa Tyr Phe Ser Ile Pro Leu Glu Leu Xaa Tyr Phe

›SEQUENCE LISTING · 26 of 53

50 55 60

Ala Thr Cys Ser Asp Xaa Phe Pro Leu Lys Trp Ile Val Leu Gln Phe

65 70 75 80

Gly Ala Phe Ile Xaa Leu Cys Gly Leu

85

<210> SEQ ID NO 99

<211> LENGTH: 2403

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 99

gctttctccc gttcaccccg cgtgcacgct ctttcccttc ctcgtgccga cgaccgggcg 60

accgccgcgc cccggcccgc gcccccttgt ctcgggccac tcgccggcaa cgagcagcca 120

ccaggaaatg ttgtgaggac tgatgcaata actaagcttg ctggatggac ggatgtgatt 180

gcatcgagcc actatggcct accgatgatc tccttgtcaa gtatcagtac atctcagact 240

tcttcatagc cctagcgtac ttctctattc cgttggagct catatatttc gtgaagaagt 300

cgtccttctt cccgtacaga tgggtcttga tccagtttgg tgcgtttata gttctytgtg 360

gggcaaccca tctgataaac ctgtggacgt tcaccacaca tacaaagacc gttgcgatgg 420

tcatgaccat agcaaaggtt tctacagcag ttgtgtcctg tgcaactgct ttgatgcttg 480

ttcatatcat ccccgactta ttgagcgtga aaactagaga gttgttcctg aagaataaag 540

ctgaagagct tgacagagag atgggactga taaggacgca ggaggagacc ggtagacatg 600

ttaggatgct tacacatgaa atcagaagta ctcttgacag gcatatgatt ttgaagacta 660

ctcttgttga gctaggaagg accttgggtc tggaagaatg tgcattgtgg atgccatctc 720

gaagtggttc aagccttcag ctttctcata ctttgcacca ccagattact gttggatcat 780

cggtgccaat taatcttcct gtcatcaatc aagtgttcag tagcaaccgg gcaattataa 840

taccccacac atctcctttg gcgcggattc gacctcttac agggcgatat gttccaccag 900

aagtggctgc agtccgtgta cctcttctcc acctttcaaa cttccaaata aatgattggc 960

ctgagctttc ggcaaaaagc tttgcaatca tggttttgat gcttccatct gatagtgcaa 1020

gaaaatggca tgtacatgaa ttggagctgg ttgaggttgt tgctgatcag gtagcagttg 1080

cactatctca tgcggctatt cttgaagagt ccatgcgagc acgtgattta ctaatggagc 1140

agaatgttgc cctggattta gctcgaagag aggctgagat ggctatccgt gctcgcaatg 1200

atttcctagc tgttatgaat cacgaaatga gaacacccat gaatgcaata atagcccttt 1260

cctccttgct tttggaaact gagcttactc ctgagcagcg tctaatggtg gaaacagtac 1320

tgaaaagcag caatttgtta gcaacactca tcaatgatgt tctggatctt tccaaactcg 1380

aggatggaag ccttgaactg gagattaaag cattcaatct tcatgctgtt ttcaaagaag 1440

tgatgggttt cattaaacca attgcatcta tcaagaggct atctgtatcg gttatgttgg 1500

caccagatct gccgttatgt gcaattggtg atgaaaagag actcatgcaa actattctga 1560

acatctctgg caatgctgta aagtttacca aggagggaca catcacgctt gtagcttcca 1620

ttgtgaaggc tgactctttg agagagttca gaaccccaga atttcatcca actgcaagtg 1680

atgaacattt ctatttgaaa gttcaggtaa aagatacagg ctgtggagtt agtcctcagg 1740

atctacctca tgtattcaca aagtttgctc atcctcaaag tggaggaaac cgagggttta 1800

atggtagtgg tcttggcctt gccatatgca agaggtttgt tagtcttatg ggagggcaca 1860

tctggatcga cagcgaagga accggaagag gttgcaccgc aacattcgtc atcaagctcg 1920

gcgtgtgtga caacacaaac acctaccagc agcagctggt tcctctagtc tggccaagca 1980

gtgcagactc caatttgtct gctccgaaag tgctgcccga cgggagagga tctgtttccc 2040

tgaaatctcg gtaccaaaga agcgtatgag ctcagtgtaa atgattgacg gcatagtgcc 2100

aagtagggga tcgattagtg ccattgtcta attttgtttg taacccagtc atagcaacat 2160

atagtgtaca aataatgtaa agccaatgga gactgcagct gtgtatctgg gtagcaacgc 2220

tgacttgctg cattgagtag tatgtcctac cagcggattg aattgcttgt tctggggtgt 2280

gcggcgcgcg ccccgttgat tgttctgttg taacttgtaa tcccatatta atcgtgtaat 2340

atgaaattca atgcaaatac acggtcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2400

aaa 2403

<210> SEQ ID NO 100

<211> LENGTH: 634

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 100

Met Asp Gly Cys Asp Cys Ile Glu Pro Leu Trp Pro Thr Asp Asp Leu

1 5 10 15

Leu Val Lys Tyr Gln Tyr Ile Ser Asp Phe Phe Ile Ala Leu Ala Tyr

20 25 30

Phe Ser Ile Pro Leu Glu Leu Ile Tyr Phe Val Lys Lys Ser Ser Phe

35 40 45

Phe Pro Tyr Arg Trp Val Leu Ile Gln Phe Gly Ala Phe Ile Val Leu

50 55 60

Cys Gly Ala Thr His Leu Ile Asn Leu Trp Thr Phe Thr Thr His Thr

65 70 75 80

Lys Thr Val Ala Met Val Met Thr Ile Ala Lys Val Ser Thr Ala Val

85 90 95

Val Ser Cys Ala Thr Ala Leu Met Leu Val His Ile Ile Pro Asp Leu

100 105 110

Leu Ser Val Lys Thr Arg Glu Leu Phe Leu Lys Asn Lys Ala Glu Glu

115 120 125

Leu Asp Arg Glu Met Gly Leu Ile Arg Thr Gln Glu Glu Thr Gly Arg

130 135 140

His Val Arg Met Leu Thr His Glu Ile Arg Ser Thr Leu Asp Arg His

145 150 155 160

Met Ile Leu Lys Thr Thr Leu Val Glu Leu Gly Arg Thr Leu Gly Leu

165 170 175

Glu Glu Cys Ala Leu Trp Met Pro Ser Arg Ser Gly Ser Ser Leu Gln

180 185 190

Leu Ser His Thr Leu His His Gln Ile Thr Val Gly Ser Ser Val Pro

195 200 205

Ile Asn Leu Pro Val Ile Asn Gln Val Phe Ser Ser Asn Arg Ala Ile

210 215 220

Ile Ile Pro His Thr Ser Pro Leu Ala Arg Ile Arg Pro Leu Thr Gly

225 230 235 240

Arg Tyr Val Pro Pro Glu Val Ala Ala Val Arg Val Pro Leu Leu His

245 250 255

Leu Ser Asn Phe Gln Ile Asn Asp Trp Pro Glu Leu Ser Ala Lys Ser

260 265 270

Phe Ala Ile Met Val Leu Met Leu Pro Ser Asp Ser Ala Arg Lys Trp

275 280 285

His Val His Glu Leu Glu Leu Val Glu Val Val Ala Asp Gln Val Ala

290 295 300

Val Ala Leu Ser His Ala Ala Ile Leu Glu Glu Ser Met Arg Ala Arg

305 310 315 320

Asp Leu Leu Met Glu Gln Asn Val Ala Leu Asp Leu Ala Arg Arg Glu

325 330 335

Ala Glu Met Ala Ile Arg Ala Arg Asn Asp Phe Leu Ala Val Met Asn

340 345 350

His Glu Met Arg Thr Pro Met Asn Ala Ile Ile Ala Leu Ser Ser Leu

355 360 365

Leu Leu Glu Thr Glu Leu Thr Pro Glu Gln Arg Leu Met Val Glu Thr

370 375 380

Val Leu Lys Ser Ser Asn Leu Leu Ala Thr Leu Ile Asn Asp Val Leu

385 390 395 400

Asp Leu Ser Lys Leu Glu Asp Gly Ser Leu Glu Leu Glu Ile Lys Ala

405 410 415

Phe Asn Leu His Ala Val Phe Lys Glu Val Met Gly Phe Ile Lys Pro

420 425 430

Ile Ala Ser Ile Lys Arg Leu Ser Val Ser Val Met Leu Ala Pro Asp

435 440 445

Leu Pro Leu Cys Ala Ile Gly Asp Glu Lys Arg Leu Met Gln Thr Ile

450 455 460

Leu Asn Ile Ser Gly Asn Ala Val Lys Phe Thr Lys Glu Gly His Ile

465 470 475 480

Thr Leu Val Ala Ser Ile Val Lys Ala Asp Ser Leu Arg Glu Phe Arg

485 490 495

Thr Pro Glu Phe His Pro Thr Ala Ser Asp Glu His Phe Tyr Leu Lys

500 505 510

Val Gln Val Lys Asp Thr Gly Cys Gly Val Ser Pro Gln Asp Leu Pro

515 520 525

His Val Phe Thr Lys Phe Ala His Pro Gln Ser Gly Gly Asn Arg Gly

530 535 540

Phe Asn Gly Ser Gly Leu Gly Leu Ala Ile Cys Lys Arg Phe Val Ser

545 550 555 560

Leu Met Gly Gly His Ile Trp Ile Asp Ser Glu Gly Thr Gly Arg Gly

565 570 575

Cys Thr Ala Thr Phe Val Ile Lys Leu Gly Val Cys Asp Asn Thr Asn

580 585 590

Thr Tyr Gln Gln Gln Leu Val Pro Leu Val Trp Pro Ser Ser Ala Asp

595 600 605

Ser Asn Leu Ser Ala Pro Lys Val Leu Pro Asp Gly Arg Gly Ser Val

610 615 620

Ser Leu Lys Ser Arg Tyr Gln Arg Ser Val

›SEQUENCE LISTING · 27 of 53

625 630

<210> SEQ ID NO 101

<211> LENGTH: 530

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (271)

<221> NAME/KEY: unsure

<222> LOCATION: (378)

<221> NAME/KEY: unsure

<222> LOCATION: (382)

<221> NAME/KEY: unsure

<222> LOCATION: (384)

<221> NAME/KEY: unsure

<222> LOCATION: (399)

<221> NAME/KEY: unsure

<222> LOCATION: (403)

<221> NAME/KEY: unsure

<222> LOCATION: (414)

<221> NAME/KEY: unsure

<222> LOCATION: (428)

<221> NAME/KEY: unsure

<222> LOCATION: (443)

<221> NAME/KEY: unsure

<222> LOCATION: (448)

<221> NAME/KEY: unsure

<222> LOCATION: (453)

<221> NAME/KEY: unsure

<222> LOCATION: (462)

<221> NAME/KEY: unsure

<222> LOCATION: (480)

<221> NAME/KEY: unsure

<222> LOCATION: (497)

<221> NAME/KEY: unsure

<222> LOCATION: (505)

<221> NAME/KEY: unsure

<222> LOCATION: (516)

<221> NAME/KEY: unsure

<222> LOCATION: (518)

<221> NAME/KEY: unsure

<222> LOCATION: (521)

<400> SEQUENCE: 101

cttacaattg cattgagccg ctctggcagg cggatgatct ccttgtaaag tatcagtaca 60

tatctgactt ctttattgcg ctcgcttact tctcgatccc tttggagctc atatatttcg 120

ttaagaagtc agcattcttc ccttaccgat gggtgcttat acaattcggc gcattcattg 180

ttctttgtgg ggcaacccac ctgataaatt tgtggacttt tgccatatat accaagacta 240

tagctgtggg tactgacagt ggcgaaagca ncgacagcgg ttgtttcgtg catcacagct 300

ttgatgcttg tgcatataat tcctgatttg ttgaatggtg aaggtttcaa gaagaagaag 360

aattttctga aagggatnag gntngatgaa gcttgatanc aanaaaatgg ggantataac 420

gaacacantg aaggagacag ggnaggantt gtncacatgc tngacccatg aagataagan 480

ggcacaactt gacaggncac aacanttcct gccgananta ngcccggccg 530

<210> SEQ ID NO 102

<211> LENGTH: 110

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (81)

<221> NAME/KEY: UNSURE

<222> LOCATION: (89)

<400> SEQUENCE: 102

Asn Cys Ile Glu Pro Leu Trp Gln Ala Asp Asp Leu Leu Val Lys Tyr

1 5 10 15

Gln Tyr Ile Ser Asp Phe Phe Ile Ala Leu Ala Tyr Phe Ser Ile Pro

20 25 30

Leu Glu Leu Ile Tyr Phe Val Lys Lys Ser Ala Phe Phe Pro Tyr Arg

35 40 45

Trp Val Leu Ile Gln Phe Gly Ala Phe Ile Val Leu Cys Gly Ala Thr

50 55 60

His Leu Ile Asn Leu Trp Thr Phe Ala Ile Tyr Thr Lys Thr Ile Ala

65 70 75 80

Xaa Val Leu Thr Val Ala Lys Ala Xaa Thr Ala Val Val Ser Cys Ile

85 90 95

Thr Ala Leu Met Leu Val His Ile Ile Pro Asp Leu Leu Asn

100 105 110

<210> SEQ ID NO 103

<211> LENGTH: 463

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (447)..(448)

<400> SEQUENCE: 103

tgtcttcacc taaccaaatg ggcgcctcca ccacctgatc cacctctgtt tcttcttcct 60

ttcatccgtt catacggggg ttagatgatg gagtcctgtg attgtataga tacgcagtat 120

ccgccggatg aacttcttgt gaagtatcag tatatctcgg atgtgctaat tgctcttgcg 180

tatttttcga ttccggtgga gctcatctat tttgttcaga agtctgcttt ctttccgtat 240

agatgggtgc ttatgcagtt tggtgccttt attgttctct gtggagcaac tcatttcata 300

aacctgtgga cattctctcc gcactctaag tctgttgctg ttgtcatgac gaatgccaaa 360

agtttcgtgt gctattgtgt catgtgcgac ggcactgatg cttgtacaca ttattcccga 420

tttgttgagt gtcaagaggc gcgaatnnat tctcaaaaaa caa 463

<210> SEQ ID NO 104

<211> LENGTH: 126

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (93)

<221> NAME/KEY: UNSURE

<222> LOCATION: (121)

<400> SEQUENCE: 104

Met Met Glu Ser Cys Asp Cys Ile Asp Thr Gln Tyr Pro Pro Asp Glu

1 5 10 15

Leu Leu Val Lys Tyr Gln Tyr Ile Ser Asp Val Leu Ile Ala Leu Ala

20 25 30

Tyr Phe Ser Ile Pro Val Glu Leu Ile Tyr Phe Val Gln Lys Ser Ala

35 40 45

Phe Phe Pro Tyr Arg Trp Val Leu Met Gln Phe Gly Ala Phe Ile Val

50 55 60

Leu Cys Gly Ala Thr His Phe Ile Asn Leu Trp Thr Phe Ser Pro His

65 70 75 80

Ser Lys Ser Val Ala Val Val Met Thr Asn Ala Lys Xaa Ser Cys Ala

85 90 95

Ile Val Ser Cys Ala Thr Ala Leu Met Leu Val His Ile Ile Pro Asp

100 105 110

Leu Leu Ser Val Lys Arg Arg Glu Xaa Ile Leu Lys Lys Gln

115 120 125

<210> SEQ ID NO 105

<211> LENGTH: 484

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (10)

<221> NAME/KEY: unsure

<222> LOCATION: (67)

<400> SEQUENCE: 105

ttcatttccn ttcatagttc tcttgtggat tgacccattt gctcaatgcc tatacttact 60

atgcccntca ctccttccca gttgtttctt tcccttactg ttgctaagtt cctcactgct 120

cttgtttctt gtgccactgc aatttccttt cccacactca ttcctctttt gctgaaaatc 180

aaagtgaggg agctattttt gaggcagaat gtgttggaat tgggccaaga ggttgggatg 240

atgaagaaac agaaggaagc tagttggcat gttaggatgc tcacttgtga gattaggaaa 300

tctctggata agcataccat cttgtatacc acacttgttg agctttccaa ggcattggat 360

ttgcacaatt gtgcagtatg gatgcctgat gaggatagga gagaaatgca tttgactcat 420

gagttgaaac caagctcagc gaggagtttt cacaattcta ttgctatcag tgatcctgat 480

gtgt 484

<210> SEQ ID NO 106

<211> LENGTH: 153

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (13)

<221> NAME/KEY: UNSURE

<222> LOCATION: (15)

<400> SEQUENCE: 106

Cys Gly Leu Thr His Leu Leu Asn Ala Tyr Thr Tyr Xaa Pro Xaa Thr

1 5 10 15

Pro Ser Gln Leu Phe Leu Ser Leu Thr Val Ala Lys Phe Leu Thr Ala

20 25 30

Leu Val Ser Cys Ala Thr Ala Ile Ser Phe Pro Thr Leu Ile Pro Leu

35 40 45

Leu Leu Lys Ile Lys Val Arg Glu Leu Phe Leu Arg Gln Asn Val Leu

50 55 60

Glu Leu Gly Gln Glu Val Gly Met Met Lys Lys Gln Lys Glu Ala Ser

65 70 75 80

Trp His Val Arg Met Leu Thr Cys Glu Ile Arg Lys Ser Leu Asp Lys

85 90 95

His Thr Ile Leu Tyr Thr Thr Leu Val Glu Leu Ser Lys Ala Leu Asp

100 105 110

Leu His Asn Cys Ala Val Trp Met Pro Asp Glu Asp Arg Arg Glu Met

115 120 125

His Leu Thr His Glu Leu Lys Pro Ser Ser Ala Arg Ser Phe His Asn

130 135 140

Ser Ile Ala Ile Ser Asp Pro Asp Val

145 150

<210> SEQ ID NO 107

<211> LENGTH: 419

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (390)

<221> NAME/KEY: unsure

<222> LOCATION: (419)

<400> SEQUENCE: 107

gttcgtcgcc cttttgccaa cctctccttc cctccctcct tcctttaccc cctctccatc 60

tagctgccgt cgccatgcca ccgccctgcc gcacccaaca ccaactccgg tgctcgcccc 120

acaagcaggg aacaagtaca ggccaggaag acaagtaaac aacaacgaca ctctatagtt 180

aggcatccag gctccagatt aactaaatgg agggctgtgc ctgcatcgaa gcagttccgg 240

cgaaactgac gaagctcctc atcaagtacc aagttaacat ctcggacttc ttcatagccc 300

tcgcctactt ctccatccca ctcgaagctg atctacttcg ccaaagaaag tcgggatcct 360

tcccctacag atgggtgctc ctccaagttn aagcggcttc attgtcctct ggcgggggn 419

<210> SEQ ID NO 108

›SEQUENCE LISTING · 28 of 53

<211> LENGTH: 62

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (41)

<221> NAME/KEY: UNSURE

<222> LOCATION: (49)

<221> NAME/KEY: UNSURE

<222> LOCATION: (61)

<400> SEQUENCE: 108

Met Glu Gly Cys Ala Cys Ile Glu Ala Val Pro Ala Lys Leu Thr Lys

1 5 10 15

Leu Leu Ile Lys Tyr Gln Val Asn Ile Ser Asp Phe Phe Ile Ala Leu

20 25 30

Ala Tyr Phe Ser Ile Pro Leu Glu Xaa Ile Tyr Phe Ala Lys Glu Ser

35 40 45

Xaa Ser Phe Pro Tyr Arg Trp Val Leu Leu Gln Val Xaa Ala

50 55 60

<210> SEQ ID NO 109

<211> LENGTH: 1100

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 109

ggcactagca gcagcatcca agactgttga ttgtctaaaa atggtgcata gtttgcatgc 60

aatttttctt gttgctggag acaataacat accgataata tatcaagttc atcgggcacg 120

tgatggatcc agctttgcca caagaaaagt ggaggcaaag cagaagggcc tagttgtatt 180

caccttgatt gcttctttcc agaaggaaga agtgggtttt gagcatcagg ctgcaatcat 240

gcctgatgtt cctccgccag aacagctcct taatctggag gagatacgtg aaagacggct 300

tactgatcca cgcttcccat cccaatatag gaacttggca gctaaaaaaa agtttattcc 360

ttggcccata gaaatgagat tttgtgaagg ttcagcgtct caacataaac caagcttaaa 420

ctactggttt agagctcgag ggaaactctc agacgaccaa gctctacaca gatgtgttgt 480

agcatatgct tcggatctac tattttctgg ggtgagcctt aaccctcatc gggagaaggg 540

tttgaagaca tactgcctca gtcttgacca ttccatctgg ttccacaaac ctgtgaaggc 600

tgacgaatgg atgctgtatg tgatcgagag cccatctgcg cacggtggtc gcggtttcgt 660

caccggacgc atgttcaaca ggcaaggaga gcttatcatg tcgctgaccc aagaggcatt 720

gattcgaagg gagaagccgc gaggaccaaa tccgaggccg aagctttgag gcacctgaca 780

gcctctgcag tcgactgtag aggatcccaa ccgagctttg agaggcgcac catcctttct 840

tctaatttgg tttagatatt tatgaattca caaacaaaaa tatagaatat caagcagtat 900

aaaagatctc aagtcaaacc taacattttt tttcatttct ccggatgatt tctatttgtt 960

ttggtgtgtg tgtggttgga ggggtattgg aagcggaagc ggaggcggag ggtttgatac 1020

tttaggctat ttcctgcagc ttactttcat tatacgaaca gtatatatac atatttaaac 1080

ttcaaaaaaa aaaaaaaaaa 1100

<210> SEQ ID NO 110

<211> LENGTH: 255

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 110

Ala Leu Ala Ala Ala Ser Lys Thr Val Asp Cys Leu Lys Met Val His

1 5 10 15

Ser Leu His Ala Ile Phe Leu Val Ala Gly Asp Asn Asn Ile Pro Ile

20 25 30

Ile Tyr Gln Val His Arg Ala Arg Asp Gly Ser Ser Phe Ala Thr Arg

35 40 45

Lys Val Glu Ala Lys Gln Lys Gly Leu Val Val Phe Thr Leu Ile Ala

50 55 60

Ser Phe Gln Lys Glu Glu Val Gly Phe Glu His Gln Ala Ala Ile Met

65 70 75 80

Pro Asp Val Pro Pro Pro Glu Gln Leu Leu Asn Leu Glu Glu Ile Arg

85 90 95

Glu Arg Arg Leu Thr Asp Pro Arg Phe Pro Ser Gln Tyr Arg Asn Leu

100 105 110

Ala Ala Lys Lys Lys Phe Ile Pro Trp Pro Ile Glu Met Arg Phe Cys

115 120 125

Glu Gly Ser Ala Ser Gln His Lys Pro Ser Leu Asn Tyr Trp Phe Arg

130 135 140

Ala Arg Gly Lys Leu Ser Asp Asp Gln Ala Leu His Arg Cys Val Val

145 150 155 160

Ala Tyr Ala Ser Asp Leu Leu Phe Ser Gly Val Ser Leu Asn Pro His

165 170 175

Arg Glu Lys Gly Leu Lys Thr Tyr Cys Leu Ser Leu Asp His Ser Ile

180 185 190

Trp Phe His Lys Pro Val Lys Ala Asp Glu Trp Met Leu Tyr Val Ile

195 200 205

Glu Ser Pro Ser Ala His Gly Gly Arg Gly Phe Val Thr Gly Arg Met

210 215 220

Phe Asn Arg Gln Gly Glu Leu Ile Met Ser Leu Thr Gln Glu Ala Leu

225 230 235 240

Ile Arg Arg Glu Lys Pro Arg Gly Pro Asn Pro Arg Pro Lys Leu

245 250 255

<210> SEQ ID NO 111

<211> LENGTH: 1414

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 111

gcacgagtgg tacgttattg cagtatcaaa ggttccttgt tcatgtttct atttttaata 60

acagtataag ctgatggtgt ccctactgta ataataaata tcttctggct tcagcttatt 120

gttagtatcc tattttgcag ctgacctgct tcgtattgcc aaatcaatat ggacacttgt 180

tgcaaccaaa gaccatttgg agtgcagaag agacacctga gaaccattct ttgctagaac 240

aaattttgca cttagagcca ttggaggtcg atatcttccg tgggtttact ttgccaggag 300

ctccgacttt taggcaagtt tttggaggac agttgatagg acaggcacta gcagcagcat 360

ccaagaccgt tgactgccta aaagcggtgc acagtttgca cgcaattttc cttatcgcag 420

gagacaagaa tttgccaata atatatcagg ttcatcgggc gcgcgatgga acaagctttg 480

ccaccagaaa agtggaggca aagcaaaagg gccttgtcat tttcaccttg attgcttcat 540

tccagaaaga cgaattaggg tttgagcatc aagctgcaat catgcctgat gttcctcctc 600

cagaagagct cctgaatctg gaagagatac gggaaagaag acttactgat cctcgcttcc 660

caatgcaata taggaactca gctgccaaga aaaagttcgt accttggccc atagaaatga 720

ggttttgtga agattcagca tctcaacata aaccaagctt aaattattgg tttagagcca 780

gaggaaaact ttctgatgat ccagcactgc ataggtgtgt tgtagcatat gcttcggatc 840

tactatattc tggtgtcagc ctaaatcctc atagggagaa gggtctgaag acatactcac 900

tcagtcttga tcactcgatt tggttccaca agcctgtgaa agctgatgat tggcttttat 960

atgtgatcga cagcccatct gcacacggtg ggcggggttt tgttacaggg cgaatgttca 1020

accgacaagg agagctcgtc atgtcgttga cccaagaggc gctgattagg agggcgaaga 1080

cgccaggaca accgtcacaa accccaaggc caaagctttg agaggtgcag aagagcagta 1140

ttatcgtttc atttttggca ctttcacagt tattttcctt ttcaatggaa gtctataaca 1200

cgtagacgtg aagccgaagt atagcatact gcatatatgt agcaagaaaa aacgatggta 1260

aaactggttc tgtaattcac gaacagctta ctgacatacc acgtatgtat tggatcggga 1320

aaaataagcg aagaacttgg tatcgctttt tttttatctc aataaagcct tcaattgttt 1380

caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 1414

<210> SEQ ID NO 112

<211> LENGTH: 331

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 112

Tyr Pro Ile Leu Gln Leu Thr Cys Phe Val Leu Pro Asn Gln Tyr Gly

1 5 10 15

His Leu Leu Gln Pro Lys Thr Ile Trp Ser Ala Glu Glu Thr Pro Glu

20 25 30

Asn His Ser Leu Leu Glu Gln Ile Leu His Leu Glu Pro Leu Glu Val

35 40 45

Asp Ile Phe Arg Gly Phe Thr Leu Pro Gly Ala Pro Thr Phe Arg Gln

50 55 60

Val Phe Gly Gly Gln Leu Ile Gly Gln Ala Leu Ala Ala Ala Ser Lys

65 70 75 80

Thr Val Asp Cys Leu Lys Ala Val His Ser Leu His Ala Ile Phe Leu

85 90 95

Ile Ala Gly Asp Lys Asn Leu Pro Ile Ile Tyr Gln Val His Arg Ala

100 105 110

Arg Asp Gly Thr Ser Phe Ala Thr Arg Lys Val Glu Ala Lys Gln Lys

115 120 125

Gly Leu Val Ile Phe Thr Leu Ile Ala Ser Phe Gln Lys Asp Glu Leu

130 135 140

Gly Phe Glu His Gln Ala Ala Ile Met Pro Asp Val Pro Pro Pro Glu

145 150 155 160

Glu Leu Leu Asn Leu Glu Glu Ile Arg Glu Arg Arg Leu Thr Asp Pro

165 170 175

Arg Phe Pro Met Gln Tyr Arg Asn Ser Ala Ala Lys Lys Lys Phe Val

180 185 190

Pro Trp Pro Ile Glu Met Arg Phe Cys Glu Asp Ser Ala Ser Gln His

195 200 205

Lys Pro Ser Leu Asn Tyr Trp Phe Arg Ala Arg Gly Lys Leu Ser Asp

210 215 220

Asp Pro Ala Leu His Arg Cys Val Val Ala Tyr Ala Ser Asp Leu Leu

›SEQUENCE LISTING · 29 of 53

225 230 235 240

Tyr Ser Gly Val Ser Leu Asn Pro His Arg Glu Lys Gly Leu Lys Thr

245 250 255

Tyr Ser Leu Ser Leu Asp His Ser Ile Trp Phe His Lys Pro Val Lys

260 265 270

Ala Asp Asp Trp Leu Leu Tyr Val Ile Asp Ser Pro Ser Ala His Gly

275 280 285

Gly Arg Gly Phe Val Thr Gly Arg Met Phe Asn Arg Gln Gly Glu Leu

290 295 300

Val Met Ser Leu Thr Gln Glu Ala Leu Ile Arg Arg Ala Lys Thr Pro

305 310 315 320

Gly Gln Pro Ser Gln Thr Pro Arg Pro Lys Leu

325 330

<210> SEQ ID NO 113

<211> LENGTH: 530

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (437)

<221> NAME/KEY: unsure

<222> LOCATION: (450)

<221> NAME/KEY: unsure

<222> LOCATION: (483)

<221> NAME/KEY: unsure

<222> LOCATION: (486)

<221> NAME/KEY: unsure

<222> LOCATION: (488)

<221> NAME/KEY: unsure

<222> LOCATION: (503)

<221> NAME/KEY: unsure

<222> LOCATION: (529)

<400> SEQUENCE: 113

gtattgttca tcctatagca actgtttttg tttgctgtga cagctatcat gcttggtgct 60

gccccatgag cactcagcat tgctgcagcc aaagtctatc tggagtgcag aaaaatctca 120

tgatacatgc tcacttgtgg agcatatatt gcatttggaa cctatagaga catcttccga 180

ggaatcactc ctacagatgc tccaacattt gggaaggtat ttggtggaca aatagttgga 240

caggcactgg ctgcggcatc aaaatctgtt gattgtctta aagttgttca tagcttgcat 300

gtctattttc ttccttgggg gggattttaa catacccatt atatatcaag ttaaagcgtc 360

tccgtgatgg gaagagtttt gctactaagg aaagtgggtg caattcaaaa gggaaatgtc 420

aaaatcactt tgctggnttc aattcacaan ggaagaagta gggttcaaca accaaggaag 480

tgnccnancc aacaatccct ggntccaaga taccgggaaa ggggactanc 530

<210> SEQ ID NO 114

<211> LENGTH: 87

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (64)

<400> SEQUENCE: 114

Met Leu Thr Cys Gly Ala Tyr Ile Ala Phe Gly Thr Tyr Arg Asp Ile

1 5 10 15

Phe Arg Gly Ile Thr Pro Thr Asp Ala Pro Thr Phe Gly Lys Val Phe

20 25 30

Gly Gly Gln Ile Val Gly Gln Ala Leu Ala Ala Ala Ser Lys Ser Val

35 40 45

Asp Cys Leu Lys Val Val His Ser Leu His Val Tyr Phe Leu Pro Xaa

50 55 60

Gly Ile Leu Thr Tyr Pro Leu Tyr Ile Lys Leu Lys Arg Leu Arg Asp

65 70 75 80

Gly Lys Ser Phe Ala Thr Lys

85

<210> SEQ ID NO 115

<211> LENGTH: 1225

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 115

gcacgaggtt tgtcaaacac agttcaccag gcagatgttg ttgctttgtc taagctcaca 60

tgcttagtgc ttccccatgt gcactcaaca ctgctgcagc caaagtctat ctggagagca 120

gaaaattctc tcgagacatg ctcacctgtg gagaatatat tgcatttgga gccccttgag 180

taataccctg gaattcagcc ttaaattaaa gcattttttt attctctttg gaaggtagat 240

atcttccaag gagttaccct tccagatgct ccaagatttg gaaaggtgtt tggggggcag 300

atgattggac aggcactggc tgcagcttca aaatctgttg actgtcttaa ggttgttcat 360

agtttgcatg cctattttat tcttgcgggg gatttaaaca tgccaattac atatcaaatt 420

caccgtctcc gtgatggaaa gagcttcgct tcaaggaaag tggatggaat tcaaaaggga 480

aatgtcatat tcactctgat ggcttcattt caaaaagaag aatcagggat ggtccaccaa 540

gaagtagcta taccatctgt ccctgctcca gataagcttc tgccgatgga agagctacgg 600

gagagacgtc ttactgaccc tcgtttacca ataacctatc ggaacaaagt agctacatct 660

caattcatcc catggcccat agagatacga ttatgtgaat atgaaactgc aacaaatatg 720

acaaaatctc ctcccagttt gagatactgg tttagagcca agggaaaact ttcagatgat 780

caagccttgc ataggtgtgt ggtggcatat gcttcagatc taatatttct tcaagtaagt 840

ttgaatcccc accgtgagaa gggtttcagg acacgttctg ttagtctgga ccactctatg 900

tggtttcacc gatccgtaaa agctgatgaa tgggtgctat ttgcgatctt tactcctagt 960

gcccatagtg cacgtgtctt tgtcaccggc caaatgttca atcagaaggg agagcttctt 1020

gtatcattgg ttcaagaagg actagccagg aaaattaatc ccggaaattc agccattaaa 1080

tctatgttat gaactgctga aaaatgcaca gattagcatg tatccatttt atagttttat 1140

ttttaaaaaa ttccatgtat ccatcaccat aacatcttag tatctaaatt atatatgtaa 1200

atttttgtat tgctctaaaa aaaaa 1225

<210> SEQ ID NO 116

<211> LENGTH: 295

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 116

Ile Lys Ala Phe Phe Tyr Ser Leu Trp Lys Val Asp Ile Phe Gln Gly

1 5 10 15

Val Thr Leu Pro Asp Ala Pro Arg Phe Gly Lys Val Phe Gly Gly Gln

20 25 30

Met Ile Gly Gln Ala Leu Ala Ala Ala Ser Lys Ser Val Asp Cys Leu

35 40 45

Lys Val Val His Ser Leu His Ala Tyr Phe Ile Leu Ala Gly Asp Leu

50 55 60

Asn Met Pro Ile Thr Tyr Gln Ile His Arg Leu Arg Asp Gly Lys Ser

65 70 75 80

Phe Ala Ser Arg Lys Val Asp Gly Ile Gln Lys Gly Asn Val Ile Phe

85 90 95

Thr Leu Met Ala Ser Phe Gln Lys Glu Glu Ser Gly Met Val His Gln

100 105 110

Glu Val Ala Ile Pro Ser Val Pro Ala Pro Asp Lys Leu Leu Pro Met

115 120 125

Glu Glu Leu Arg Glu Arg Arg Leu Thr Asp Pro Arg Leu Pro Ile Thr

130 135 140

Tyr Arg Asn Lys Val Ala Thr Ser Gln Phe Ile Pro Trp Pro Ile Glu

145 150 155 160

Ile Arg Leu Cys Glu Tyr Glu Thr Ala Thr Asn Met Thr Lys Ser Pro

165 170 175

Pro Ser Leu Arg Tyr Trp Phe Arg Ala Lys Gly Lys Leu Ser Asp Asp

180 185 190

Gln Ala Leu His Arg Cys Val Val Ala Tyr Ala Ser Asp Leu Ile Phe

195 200 205

Leu Gln Val Ser Leu Asn Pro His Arg Glu Lys Gly Phe Arg Thr Arg

210 215 220

Ser Val Ser Leu Asp His Ser Met Trp Phe His Arg Ser Val Lys Ala

225 230 235 240

Asp Glu Trp Val Leu Phe Ala Ile Phe Thr Pro Ser Ala His Ser Ala

245 250 255

Arg Val Phe Val Thr Gly Gln Met Phe Asn Gln Lys Gly Glu Leu Leu

260 265 270

Val Ser Leu Val Gln Glu Gly Leu Ala Arg Lys Ile Asn Pro Gly Asn

275 280 285

Ser Ala Ile Lys Ser Met Leu

290 295

<210> SEQ ID NO 117

<211> LENGTH: 1756

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 117

gtaagttcac catgctgatg ttgtagcttt gacaaagcta tcatgcttgg tgctgcccca 60

tgagcactca gcattgctgc agccaaagtc tatttggagt gcagaaaaat ctcatgatac 120

atgctcactt gtggagcata tattgcattt ggaccctata gaggtagaca tcttccgagg 180

aatcactcct ccagatgctc caacatttgg gaaggtattt ggtggacaaa tagttggaca 240

ggcactggct gcagcatcaa aatctgttga ttgtcgtaaa gttgttcata gcttgcatgt 300

ctattttctt cttgtggggg attttaacat acccattata tatcaagtta agcgtctccg 360

tgatgggaag agttttgcta caaggaaagt ggatgcaatt caaaagggaa atgtcatatt 420

cactttgttg gcttcatttc ataaggaaga agtagggttt caccaccagg aagtgtctat 480

cccatcagtc cctgctccag atacgctttt atcgttggaa gagcttcggg agcaacgtct 540

tactgaccct cgtcttccaa gaacttaccg gaacaaggtt gctacaattg aattcattcc 600

ctggcccata gagatacggt tctgtgaacc taaaatttca acaaatcaga ccaaatctcc 660

tcctagtttg agatactggt ttagagcaag gggaaaactt tcagatgatc aagccttgca 720

taggtgtgtg gtagcatata catcagatct aatcttcctt caagtgagtt tgaaccccaa 780

ccgtaggaag ggaaggaagg ctcgtgctgt gagtctggac cactccatgt ggtttcacag 840

›SEQUENCE LISTING · 30 of 53

acctttaaga gctgatgatt ggatactatt tgtgatcttt agtcctactg ccaataatgc 900

ccgcggctat gtcactggcc aaatgttcaa tcagaaggga gagcatcttg tgtctgtggt 960

tcaagaaggt gtaatgaggg aagttatttc tgctaagtca gccatcaaat ctaatctatg 1020

aatagctaaa aatgcactga aacttgtgtt ctttttagtt ttgtcttctt ttgttttcct 1080

tctatagtga atgatcgggt gaaccataca ataggtggtg caccactata tcaccatcat 1140

atagtgcacc tttattttta atcaacggtt caaaattgtg attgacaagt aattgatgtg 1200

ttagatttat ttacaattaa ctagttttaa aatttacaaa ttggtccttt tgttgtcgcg 1260

tttgtaccat ctcctacaaa gattcacgcc tctgtcggct cttccatcac cacgagccct 1320

gatctctgct ggcatccgca acaaccatgg ctcatctctt catttcaaaa gttatagctt 1380

tacttcatca atcactatac aaccagaatt gccaattcaa atgtaagaaa aatatacttt 1440

tttttatgga caaatgatac atataacatt acctaccagt atagtcttgc ttcctaagtc 1500

ttgggagaag acaaatgatg aattctgggt ttgttttggg tccttatgct ttcaaaaaca 1560

cgttgaacac gaattgttat ttatttggat tacactaaac gtgctctaac atgtaacgca 1620

aatacaaata ttcccagagt cattgaaatg ttattctagt ggaaagaact tgactatcca 1680

tatttggagg aaattaattt taagcccaaa ataatacgag atatgttaca caaaaaaaaa 1740

aaaaaaaaaa aaaaaa 1756

<210> SEQ ID NO 118

<211> LENGTH: 338

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 118

Val His His Ala Asp Val Val Ala Leu Thr Lys Leu Ser Cys Leu Val

1 5 10 15

Leu Pro His Glu His Ser Ala Leu Leu Gln Pro Lys Ser Ile Trp Ser

20 25 30

Ala Glu Lys Ser His Asp Thr Cys Ser Leu Val Glu His Ile Leu His

35 40 45

Leu Asp Pro Ile Glu Val Asp Ile Phe Arg Gly Ile Thr Pro Pro Asp

50 55 60

Ala Pro Thr Phe Gly Lys Val Phe Gly Gly Gln Ile Val Gly Gln Ala

65 70 75 80

Leu Ala Ala Ala Ser Lys Ser Val Asp Cys Arg Lys Val Val His Ser

85 90 95

Leu His Val Tyr Phe Leu Leu Val Gly Asp Phe Asn Ile Pro Ile Ile

100 105 110

Tyr Gln Val Lys Arg Leu Arg Asp Gly Lys Ser Phe Ala Thr Arg Lys

115 120 125

Val Asp Ala Ile Gln Lys Gly Asn Val Ile Phe Thr Leu Leu Ala Ser

130 135 140

Phe His Lys Glu Glu Val Gly Phe His His Gln Glu Val Ser Ile Pro

145 150 155 160

Ser Val Pro Ala Pro Asp Thr Leu Leu Ser Leu Glu Glu Leu Arg Glu

165 170 175

Gln Arg Leu Thr Asp Pro Arg Leu Pro Arg Thr Tyr Arg Asn Lys Val

180 185 190

Ala Thr Ile Glu Phe Ile Pro Trp Pro Ile Glu Ile Arg Phe Cys Glu

195 200 205

Pro Lys Ile Ser Thr Asn Gln Thr Lys Ser Pro Pro Ser Leu Arg Tyr

210 215 220

Trp Phe Arg Ala Arg Gly Lys Leu Ser Asp Asp Gln Ala Leu His Arg

225 230 235 240

Cys Val Val Ala Tyr Thr Ser Asp Leu Ile Phe Leu Gln Val Ser Leu

245 250 255

Asn Pro Asn Arg Arg Lys Gly Arg Lys Ala Arg Ala Val Ser Leu Asp

260 265 270

His Ser Met Trp Phe His Arg Pro Leu Arg Ala Asp Asp Trp Ile Leu

275 280 285

Phe Val Ile Phe Ser Pro Thr Ala Asn Asn Ala Arg Gly Tyr Val Thr

290 295 300

Gly Gln Met Phe Asn Gln Lys Gly Glu His Leu Val Ser Val Val Gln

305 310 315 320

Glu Gly Val Met Arg Glu Val Ile Ser Ala Lys Ser Ala Ile Lys Ser

325 330 335

Asn Leu

<210> SEQ ID NO 119

<211> LENGTH: 1083

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (986)

<221> NAME/KEY: unsure

<222> LOCATION: (1014)

<221> NAME/KEY: unsure

<222> LOCATION: (1017)

<221> NAME/KEY: unsure

<222> LOCATION: (1019)

<221> NAME/KEY: unsure

<222> LOCATION: (1022)

<400> SEQUENCE: 119

gcacgagaga ctgttgattg tctaaaaatg gtgcatagtt tgcatgcaat ttttcttgtt 60

gctggagaca ataacatacc gataatatat caagttcatc gggcacgtga tggatccagc 120

tttgccacaa gaaaagtgga ggcaaagcag aagggcctag ttgtattcac cttgattgct 180

tctttccaga aggaagaagt gggttttgag catcaggctg caatcatgcc tgatgttcct 240

ccgccagaac agctccttaa tctggaggag atacgtgaaa gacggcttac tgatccacgc 300

ttcccatccc aatataggaa cttggcagct aaaaaaaagt ttattccttg gcccatagaa 360

atgagatttt gtgaaggttc agcgtctcaa cataaaccaa gcttaaacta ctggtttaga 420

gctcgaggga aactctcaga cgaccaagct ctacacagat gtgttgtagc atatgcttcg 480

gatctactat tttctggggt gagccttaac cctcatcggg agaagggttt gaagacatac 540

tgcctcagtc ttgaccattc catctggttc cacaaacctg tgaaggctga cgaatggatg 600

ctgtatgtga tcgagagccc atctgcgcac ggtggtcgcg gtttcgtcac cggacgcatg 660

ttcaacaggc aaggagagct tatcatgtcg ctgacccaag aggcattgat tcgaagggag 720

aagccgcgag gaccaaatcc gaggccgaag ctttgaggca cctgacagcc tctgcagtcg 780

actgtagagg atcccaaccg agctttgaga ggcgcaccat cctttcttct aatttggttt 840

agatatttat gaattcacaa acaaaaatat agaatatcaa gcagtataaa agatctcaag 900

tcaaacctaa catttttttt tcatttctcc ggatgatttc tatttgtttt ggtgtgtgtg 960

tggttggagg ggtattggaa gcggangcgg aggcggaggg tttgatactt tagnctntnt 1020

cntgcagctt actaattctc gtacacatat ttaaatttca ttatacgaac agtatatata 1080

caa 1083

<210> SEQ ID NO 120

<211> LENGTH: 251

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 120

Ala Arg Glu Thr Val Asp Cys Leu Lys Met Val His Ser Leu His Ala

1 5 10 15

Ile Phe Leu Val Ala Gly Asp Asn Asn Ile Pro Ile Ile Tyr Gln Val

20 25 30

His Arg Ala Arg Asp Gly Ser Ser Phe Ala Thr Arg Lys Val Glu Ala

35 40 45

Lys Gln Lys Gly Leu Val Val Phe Thr Leu Ile Ala Ser Phe Gln Lys

50 55 60

Glu Glu Val Gly Phe Glu His Gln Ala Ala Ile Met Pro Asp Val Pro

65 70 75 80

Pro Pro Glu Gln Leu Leu Asn Leu Glu Glu Ile Arg Glu Arg Arg Leu

85 90 95

Thr Asp Pro Arg Phe Pro Ser Gln Tyr Arg Asn Leu Ala Ala Lys Lys

100 105 110

Lys Phe Ile Pro Trp Pro Ile Glu Met Arg Phe Cys Glu Gly Ser Ala

115 120 125

Ser Gln His Lys Pro Ser Leu Asn Tyr Trp Phe Arg Ala Arg Gly Lys

130 135 140

Leu Ser Asp Asp Gln Ala Leu His Arg Cys Val Val Ala Tyr Ala Ser

145 150 155 160

Asp Leu Leu Phe Ser Gly Val Ser Leu Asn Pro His Arg Glu Lys Gly

165 170 175

Leu Lys Thr Tyr Cys Leu Ser Leu Asp His Ser Ile Trp Phe His Lys

180 185 190

Pro Val Lys Ala Asp Glu Trp Met Leu Tyr Val Ile Glu Ser Pro Ser

195 200 205

Ala His Gly Gly Arg Gly Phe Val Thr Gly Arg Met Phe Asn Arg Gln

210 215 220

Gly Glu Leu Ile Met Ser Leu Thr Gln Glu Ala Leu Ile Arg Arg Glu

225 230 235 240

Lys Pro Arg Gly Pro Asn Pro Arg Pro Lys Leu

245 250

<210> SEQ ID NO 121

<211> LENGTH: 1312

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (1197)

<221> NAME/KEY: unsure

<222> LOCATION: (1216)

<221> NAME/KEY: unsure

<222> LOCATION: (1234)

<400> SEQUENCE: 121

tggtacgtta ttgcagtatc aaaggttcct tgttcatgtt tctattttta ataacagtat 60

aagctgatgg tgtccctact gtaataataa atatcttctg gcttcagctt attgttagta 120

tcctattttg cagctgacct gcttcgtatt gccaaatcaa tatggacact tgttgcaacc 180

›SEQUENCE LISTING · 31 of 53

aaagaccatt tggagtgcag aagagacacc tgagaaccat tctttgctag aacaaatttt 240

gcacttagag ccattggagg tcgatatctt ccgtgggttt actttgccaa ggagctccac 300

ttttaggcaa gtttttggag gacagttgat aggacaggca ctagcagcag catccaagac 360

cgttgactgc ctaaaagcgg tgcacagttt gcacgcaatt ttccttatcg caggagacaa 420

gaatttgcca ataatatatc aggttcatcg ggcgcgcgat ggaacaagct ttgccaccag 480

aaaagtggag gcaaagcaaa agggccttgt cattttcacc ttgattgctt cattccagaa 540

agacgaatta gggtttgagc atcaagctgc aatcatgcct gatgttcctc ctccagaaga 600

gctcctgaat ctggaagaca tacaggaaag aagacttact gatcctcgct taccagcata 660

taggaactca gctgccaaga aaaagttcgt accttggccc atagaaatga ggttttgtga 720

agattcagca tctcaacata aaccaagctt aaattattgg tttagagcca gaggaaaact 780

ttctgatgat ccagcactgc ataggtgtgt tgtagcatat gcttcggatc tactatattc 840

tggtgtcagc ctaaatcctc atagggagaa gggtctgaag acatactcac tcagtcttga 900

tcactcgatt tggttccaca agcctgtgaa agctgatgat tggcttttat atgtgatcga 960

cagcccatct gcacacggtg ggcggggttt tgttacaggg cgaatgttca accgacaagg 1020

agagctcgtc atgtcgttga cccaagaggc gctgattagg agggcgaaga cgccaggaca 1080

accgtcacaa accccaaggc caaagctttg agaggtgcag aagagcagta ttatcgtttc 1140

atttttggca ctttcacaag ttattttcct tttcaatgga aagtctataa cacgtanacg 1200

tgaagccgaa gtatancata ctgcatatat gtancaagaa aaaacgatgg taaaactggt 1260

tctgttattc acgaacaact tactggacat accaacgtat gtatggatcg gg 1312

<210> SEQ ID NO 122

<211> LENGTH: 330

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 122

Tyr Pro Ile Leu Gln Leu Thr Cys Phe Val Leu Pro Asn Gln Tyr Gly

1 5 10 15

His Leu Leu Gln Pro Lys Thr Ile Trp Ser Ala Glu Glu Thr Pro Glu

20 25 30

Asn His Ser Leu Leu Glu Gln Ile Leu His Leu Glu Pro Leu Glu Val

35 40 45

Asp Ile Phe Arg Gly Phe Thr Leu Pro Arg Ser Ser Thr Phe Arg Gln

50 55 60

Val Phe Gly Gly Gln Leu Ile Gly Gln Ala Leu Ala Ala Ala Ser Lys

65 70 75 80

Thr Val Asp Cys Leu Lys Ala Val His Ser Leu His Ala Ile Phe Leu

85 90 95

Ile Ala Gly Asp Lys Asn Leu Pro Ile Ile Tyr Gln Val His Arg Ala

100 105 110

Arg Asp Gly Thr Ser Phe Ala Thr Arg Lys Val Glu Ala Lys Gln Lys

115 120 125

Gly Leu Val Ile Phe Thr Leu Ile Ala Ser Phe Gln Lys Asp Glu Leu

130 135 140

Gly Phe Glu His Gln Ala Ala Ile Met Pro Asp Val Pro Pro Pro Glu

145 150 155 160

Glu Leu Leu Asn Leu Glu Asp Ile Gln Glu Arg Arg Leu Thr Asp Pro

165 170 175

Arg Leu Pro Ala Tyr Arg Asn Ser Ala Ala Lys Lys Lys Phe Val Pro

180 185 190

Trp Pro Ile Glu Met Arg Phe Cys Glu Asp Ser Ala Ser Gln His Lys

195 200 205

Pro Ser Leu Asn Tyr Trp Phe Arg Ala Arg Gly Lys Leu Ser Asp Asp

210 215 220

Pro Ala Leu His Arg Cys Val Val Ala Tyr Ala Ser Asp Leu Leu Tyr

225 230 235 240

Ser Gly Val Ser Leu Asn Pro His Arg Glu Lys Gly Leu Lys Thr Tyr

245 250 255

Ser Leu Ser Leu Asp His Ser Ile Trp Phe His Lys Pro Val Lys Ala

260 265 270

Asp Asp Trp Leu Leu Tyr Val Ile Asp Ser Pro Ser Ala His Gly Gly

275 280 285

Arg Gly Phe Val Thr Gly Arg Met Phe Asn Arg Gln Gly Glu Leu Val

290 295 300

Met Ser Leu Thr Gln Glu Ala Leu Ile Arg Arg Ala Lys Thr Pro Gly

305 310 315 320

Gln Pro Ser Gln Thr Pro Arg Pro Lys Leu

325 330

<210> SEQ ID NO 123

<211> LENGTH: 627

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (507)..(508)

<221> NAME/KEY: unsure

<222> LOCATION: (512)

<221> NAME/KEY: unsure

<222> LOCATION: (542)

<221> NAME/KEY: unsure

<222> LOCATION: (545)

<221> NAME/KEY: unsure

<222> LOCATION: (553)

<221> NAME/KEY: unsure

<222> LOCATION: (570)

<221> NAME/KEY: unsure

<222> LOCATION: (577)

<221> NAME/KEY: unsure

<222> LOCATION: (580)

<400> SEQUENCE: 123

gtttgtcaaa cacagttcac caggcagatg ttgttgcttt gtctaagctc acatgcttag 60

tgcttcccca tgtgcactca acactgctgc agccaaagtc tatctggaga gcagaaaatt 120

ctctcgagac atgctcacct gtggagaata tattgcattt ggagcccctt gagtaatacc 180

ctggaattca gccttaaatt aaagcatttt tttattctct ttggaaggta gatatcttcc 240

aaggagttac ccttccagat gctccaagat ttggaaaggt gtttgggggg cagatgattg 300

gacaggcact ggctgcagct tcaaaatctg ttgactgtct taaggttgtt caaagtttgc 360

atgcctattt tattcttgcg ggggatttaa acatgccaat tacatatcag attcaccgtc 420

tccgtgatgg aaagagcttc gcttcacgga aagtggatgg aattcaaaag ggaaatgtca 480

tattcactct gatggcttca tttcaannag angaatcagg gatggcccac caagaagtaa 540

cnatnccatc tgnccctgct ccaagataan cttctgncgn tgggagaggc tacgggagag 600

acgtcttact ggacccctcg tttacca 627

<210> SEQ ID NO 124

<211> LENGTH: 123

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (104)..(105)

<221> NAME/KEY: UNSURE

<222> LOCATION: (116)

<221> NAME/KEY: UNSURE

<222> LOCATION: (119)

<400> SEQUENCE: 124

Ile Lys Ala Phe Phe Tyr Ser Leu Trp Lys Val Asp Ile Phe Gln Gly

1 5 10 15

Val Thr Leu Pro Asp Ala Pro Arg Phe Gly Lys Val Phe Gly Gly Gln

20 25 30

Met Ile Gly Gln Ala Leu Ala Ala Ala Ser Lys Ser Val Asp Cys Leu

35 40 45

Lys Val Val Gln Ser Leu His Ala Tyr Phe Ile Leu Ala Gly Asp Leu

50 55 60

Asn Met Pro Ile Thr Tyr Gln Ile His Arg Leu Arg Asp Gly Lys Ser

65 70 75 80

Phe Ala Ser Arg Lys Val Asp Gly Ile Gln Lys Gly Asn Val Ile Phe

85 90 95

Thr Leu Met Ala Ser Phe Gln Xaa Xaa Glu Ser Gly Met Ala His Gln

100 105 110

Glu Val Thr Xaa Pro Ser Xaa Pro Ala Pro Arg

115 120

<210> SEQ ID NO 125

<211> LENGTH: 477

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (428)

<221> NAME/KEY: unsure

<222> LOCATION: (460)

<221> NAME/KEY: unsure

<222> LOCATION: (469)

<221> NAME/KEY: unsure

<222> LOCATION: (471)

<221> NAME/KEY: unsure

<222> LOCATION: (477)

<400> SEQUENCE: 125

ccatgctgat gttgtagctt tgacaaagct atcatgcttg gtgctgcccc atgagcactc 60

agcattgctg cagccaaagt ctatttggag tgcagaaaaa tctcatgata catgctcact 120

tgtggagcat atattgcatt tggaccctat agaggtagac atcttccgag gaatcactcc 180

tccagatgct ccaacatttg ggaaggtatt tggtggacaa atagttggac aggcactggc 240

tgcagcatca aaatctgttg attgtcgtaa agttgttcat agcttgcatg tctattttct 300

tcttgtgggg gattttaaca tacccattat atatcaagtt aagcgtctcc gtgatgggaa 360

gagttttgct acaaggaaag tggatgcaat tcaaaaggga aatgtcatat tcactttgtt 420

ggcttcantt cataaaggaa gaagtagggt tcaccaccan gaagtgtcna nccatcn 477

<210> SEQ ID NO 126

<211> LENGTH: 158

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (143)

<221> NAME/KEY: UNSURE

<222> LOCATION: (153)

<221> NAME/KEY: UNSURE

<222> LOCATION: (157)

›SEQUENCE LISTING · 32 of 53

<400> SEQUENCE: 126

His Ala Asp Val Val Ala Leu Thr Lys Leu Ser Cys Leu Val Leu Pro

1 5 10 15

His Glu His Ser Ala Leu Leu Gln Pro Lys Ser Ile Trp Ser Ala Glu

20 25 30

Lys Ser His Asp Thr Cys Ser Leu Val Glu His Ile Leu His Leu Asp

35 40 45

Pro Ile Glu Val Asp Ile Phe Arg Gly Ile Thr Pro Pro Asp Ala Pro

50 55 60

Thr Phe Gly Lys Val Phe Gly Gly Gln Ile Val Gly Gln Ala Leu Ala

65 70 75 80

Ala Ala Ser Lys Ser Val Asp Cys Arg Lys Val Val His Ser Leu His

85 90 95

Val Tyr Phe Leu Leu Val Gly Asp Phe Asn Ile Pro Ile Ile Tyr Gln

100 105 110

Val Lys Arg Leu Arg Asp Gly Lys Ser Phe Ala Thr Arg Lys Val Asp

115 120 125

Ala Ile Gln Lys Gly Asn Val Ile Phe Thr Leu Leu Ala Ser Xaa His

130 135 140

Lys Gly Arg Ser Arg Val His His Xaa Glu Val Ser Xaa His

145 150 155

<210> SEQ ID NO 127

<211> LENGTH: 1663

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (1623)..(1624)

<400> SEQUENCE: 127

gcacgaggca tgcaattgat ggtattccta agaactgcca gatattgcat gttgagcaag 60

aggtcgtggg tgacgatacg acagctttgc agtgtgttct gaacgccgat gtcgaacgag 120

ttcaactttt gcaggaagaa gctcgtcttg ttcaacaaca gaaagattta gagattgagg 180

ctgagtttgg gcagagctct gacaagggca aggatggttt tgacaaagat tctatcagca 240

agaggcttga ggagatatat aaacggcttg agctcattga tgcggatgca gcagaggctc 300

gcgcagcatc aattttggcg ggtcttagtt ttactccaga aatgcaacgc aagcgtacaa 360

aacaattttc tggtggatgg cgcatgagaa tagctctagc acgtgctctg ttcattgagc 420

ctgatttgct actacttgat gagccgacaa accatcttga tcttcatgct gtgttatggc 480

tagaaactta tctcctgaag tggccaaaga cattcattgt tgtatcacac gcaagggagt 540

ttttgaatac ggttgtcacc gatatccttc atctgcatgg tagaaaacta catgcttaca 600

aaggtgacta tgacacattt gagaggacaa gggaagagca ccttaagaat cagcagaaag 660

cctttgaaac aaatgagaag gccagacagc acatgcagac attcattgac aagtttcggt 720

acaatgcaaa gagagcatca cttgttcaat caagaatcaa ggcattggag cgaatggaac 780

atgttgatgc agttgtcagc gatccagact ataaatttga atttccaacc ccagatgacc 840

gccctgggcc accaatcatc agcttcagtg atgcctcatt tggttaccct ggagggccta 900

ccttgtttaa aaatttgaat tttggtatcg acctggacag ccgcatagca atggttggtc 960

ctaatggcat tggcaagtcc actatactga aactaatttc tggggatctg cagccaactt 1020

ctggaacagt atttcgctct cccaaggttc gcatggctgt attcaatcag catcatgttg 1080

atggccttga tttgacggtg aatccccttt tgtacatgat gaagtgctat ccgggtgtgc 1140

cggagcagaa gttgagggca catctgggtt cgtttggtgt ttcgggaagc cttgccctcc 1200

aatctatgta cactttatca ggtggtcaga agagtagggt tgcattcgcg aaaataacct 1260

tcaagaagcc gcacatcatt ctccttgacg agccttctaa ccatcttgat ctggacgctg 1320

ttgaggcgct catccagggt ttactcattt ttcagggagg ggtgctgatg gtgagtcacg 1380

atgagcatct tatcactgga agcgtggacg agctttgggt ggtgtcggaa ggcagggtga 1440

ccccgttctc gggcactttc aaggactaca agaagatgct caagtaacaa aaacatgtat 1500

tatcgaccat agcctatata tctatatgct tgctattatg ttgtattacc gtataaattt 1560

tgggagtaaa gcatcgaaat tattataaca catacctaat gccgaaagga gaagatttct 1620

cannaaaaaa aaaaaaaaaa taaaaaaaaa aaaaaaaaaa aaa 1663

<210> SEQ ID NO 128

<211> LENGTH: 494

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 128

Thr Arg His Ala Ile Asp Gly Ile Pro Lys Asn Cys Gln Ile Leu His

1 5 10 15

Val Glu Gln Glu Val Val Gly Asp Asp Thr Thr Ala Leu Gln Cys Val

20 25 30

Leu Asn Ala Asp Val Glu Arg Val Gln Leu Leu Gln Glu Glu Ala Arg

35 40 45

Leu Val Gln Gln Gln Lys Asp Leu Glu Ile Glu Ala Glu Phe Gly Gln

50 55 60

Ser Ser Asp Lys Gly Lys Asp Gly Phe Asp Lys Asp Ser Ile Ser Lys

65 70 75 80

Arg Leu Glu Glu Ile Tyr Lys Arg Leu Glu Leu Ile Asp Ala Asp Ala

85 90 95

Ala Glu Ala Arg Ala Ala Ser Ile Leu Ala Gly Leu Ser Phe Thr Pro

100 105 110

Glu Met Gln Arg Lys Arg Thr Lys Gln Phe Ser Gly Gly Trp Arg Met

115 120 125

Arg Ile Ala Leu Ala Arg Ala Leu Phe Ile Glu Pro Asp Leu Leu Leu

130 135 140

Leu Asp Glu Pro Thr Asn His Leu Asp Leu His Ala Val Leu Trp Leu

145 150 155 160

Glu Thr Tyr Leu Leu Lys Trp Pro Lys Thr Phe Ile Val Val Ser His

165 170 175

Ala Arg Glu Phe Leu Asn Thr Val Val Thr Asp Ile Leu His Leu His

180 185 190

Gly Arg Lys Leu His Ala Tyr Lys Gly Asp Tyr Asp Thr Phe Glu Arg

195 200 205

Thr Arg Glu Glu His Leu Lys Asn Gln Gln Lys Ala Phe Glu Thr Asn

210 215 220

Glu Lys Ala Arg Gln His Met Gln Thr Phe Ile Asp Lys Phe Arg Tyr

225 230 235 240

Asn Ala Lys Arg Ala Ser Leu Val Gln Ser Arg Ile Lys Ala Leu Glu

245 250 255

Arg Met Glu His Val Asp Ala Val Val Ser Asp Pro Asp Tyr Lys Phe

260 265 270

Glu Phe Pro Thr Pro Asp Asp Arg Pro Gly Pro Pro Ile Ile Ser Phe

275 280 285

Ser Asp Ala Ser Phe Gly Tyr Pro Gly Gly Pro Thr Leu Phe Lys Asn

290 295 300

Leu Asn Phe Gly Ile Asp Leu Asp Ser Arg Ile Ala Met Val Gly Pro

305 310 315 320

Asn Gly Ile Gly Lys Ser Thr Ile Leu Lys Leu Ile Ser Gly Asp Leu

325 330 335

Gln Pro Thr Ser Gly Thr Val Phe Arg Ser Pro Lys Val Arg Met Ala

340 345 350

Val Phe Asn Gln His His Val Asp Gly Leu Asp Leu Thr Val Asn Pro

355 360 365

Leu Leu Tyr Met Met Lys Cys Tyr Pro Gly Val Pro Glu Gln Lys Leu

370 375 380

Arg Ala His Leu Gly Ser Phe Gly Val Ser Gly Ser Leu Ala Leu Gln

385 390 395 400

Ser Met Tyr Thr Leu Ser Gly Gly Gln Lys Ser Arg Val Ala Phe Ala

405 410 415

Lys Ile Thr Phe Lys Lys Pro His Ile Ile Leu Leu Asp Glu Pro Ser

420 425 430

Asn His Leu Asp Leu Asp Ala Val Glu Ala Leu Ile Gln Gly Leu Leu

435 440 445

Ile Phe Gln Gly Gly Val Leu Met Val Ser His Asp Glu His Leu Ile

450 455 460

Thr Gly Ser Val Asp Glu Leu Trp Val Val Ser Glu Gly Arg Val Thr

465 470 475 480

Pro Phe Ser Gly Thr Phe Lys Asp Tyr Lys Lys Met Leu Lys

485 490

<210> SEQ ID NO 129

<211> LENGTH: 1875

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 129

ctggaatgcc tccagtattt gtaaatcaca ataactctgg tgggccagct gttagggata 60

tccatatgga gaacttcagt gttactgttg gtggtcgtga tctcattcaa gattgcactg 120

taacacttgc ttttgggagg cactatggtc ttgttggaag aaatggtaca gggaaaacct 180

cttttctcag agctatggcg atgcatgcga ttgatgggat tcccaagaac tgccagatat 240

tgcatgttga gcaagaggtt gtgggcgatg acacaacagc tttgcagtgt gttctgaatg 300

ctgatattga acgggttcaa cttttgcaag aagaagctca tctggttcaa cggcagaaag 360

atctagagta tgaggctgag tttgagcaga gcgcgtccaa aagcaaggat ggtcttgaca 420

aagatgctat cagcaagagg cttgaggaga tatacaaacg ccttgagttt attgatgctg 480

atgctgcaga ggctcgtgca gcatcaattc tggcgggtct tagtttcact cctgaaatgc 540

aacgtaaacg cacaaaacag ttttctggtg gatggcgcat gagaattgct ctagcacgtg 600

ctctcttcat tgagcctgat ttgttgctac ttgatgagcc aacaaatcat cttgatctcc 660

›SEQUENCE LISTING · 33 of 53

atgctgtgtt atggttagaa acatatctcc tgaaatggcc aaagacattc attgttgtat 720

cacatgctag ggagtttctt aatacggtcg tcaccgacat tcttcatctg catggacaaa 780

agctacatgc ttacaaaggt gattatgaca cattcgagag gacaagggag gagcacctta 840

agaaccagca gaaagctttt gaaacaaatg agaaggccag atcccacatg caggcattca 900

ttgacaagtt ccgttacaat gcaaagagag catcacttgt tcaatcaaga atcaaggcat 960

tggagcgaat ggagcatgtt gatgcggtcg tcagtgatcc agactataaa ttcgaatttc 1020

caacaccaga tgaccgccct ggaccaccaa tcatcagctt cagtgacgca tcttttggtt 1080

atcctggagg ccccacctta tttaaaaact tgaattttgg tattgacctg gacagccgca 1140

tagcaatggt tggtccaaat ggtattggca aatccactat acttaaacta atatctggag 1200

atcttcagcc aacttcaggg acagtgttcc gctctcccaa ggttcgcatg gctgtattca 1260

atcaacacca tgttgatggg cttgatttaa cagtgaaccc ccttttgtac atgatgagat 1320

gctaccctgg tgtacctgaa cagaaactga gggcacactt gggttctttc ggtgttacag 1380

gaaatcttgc cctccaacca atgtacactt tatcaggtgg tcagaaaagt agggttgcct 1440

ttgcaaagat caccttcaag aagccacaca ttattcttct cgatgagcct tctaaccatc 1500

ttgatctgga tgctgtggag gcgctgatcc agggtttact cgttttccag ggaggagtgc 1560

taatggtgag tcacgacgag cacctgataa cggggagcgt ggatgagctt tgggtggtgt 1620

cggaaggcag ggtgtcgcct ttcgcaggca cgttcaagga ctacaagaag atgctcaagt 1680

cttaatctag agccagtgat gatatatata cagagacagc gagtctacaa agaaagacac 1740

caacatatat atctgtatcg tgcagcaccg atgtaacatt taccggcctg aggccaaatc 1800

atcatttatg tttatttcat gtggatttac tggggaaaaa aaaaaaaaaa aaaaaaaaaa 1860

aaaaaaaaaa aaaaa 1875

<210> SEQ ID NO 130

<211> LENGTH: 560

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 130

Gly Met Pro Pro Val Phe Val Asn His Asn Asn Ser Gly Gly Pro Ala

1 5 10 15

Val Arg Asp Ile His Met Glu Asn Phe Ser Val Thr Val Gly Gly Arg

20 25 30

Asp Leu Ile Gln Asp Cys Thr Val Thr Leu Ala Phe Gly Arg His Tyr

35 40 45

Gly Leu Val Gly Arg Asn Gly Thr Gly Lys Thr Ser Phe Leu Arg Ala

50 55 60

Met Ala Met His Ala Ile Asp Gly Ile Pro Lys Asn Cys Gln Ile Leu

65 70 75 80

His Val Glu Gln Glu Val Val Gly Asp Asp Thr Thr Ala Leu Gln Cys

85 90 95

Val Leu Asn Ala Asp Ile Glu Arg Val Gln Leu Leu Gln Glu Glu Ala

100 105 110

His Leu Val Gln Arg Gln Lys Asp Leu Glu Tyr Glu Ala Glu Phe Glu

115 120 125

Gln Ser Ala Ser Lys Ser Lys Asp Gly Leu Asp Lys Asp Ala Ile Ser

130 135 140

Lys Arg Leu Glu Glu Ile Tyr Lys Arg Leu Glu Phe Ile Asp Ala Asp

145 150 155 160

Ala Ala Glu Ala Arg Ala Ala Ser Ile Leu Ala Gly Leu Ser Phe Thr

165 170 175

Pro Glu Met Gln Arg Lys Arg Thr Lys Gln Phe Ser Gly Gly Trp Arg

180 185 190

Met Arg Ile Ala Leu Ala Arg Ala Leu Phe Ile Glu Pro Asp Leu Leu

195 200 205

Leu Leu Asp Glu Pro Thr Asn His Leu Asp Leu His Ala Val Leu Trp

210 215 220

Leu Glu Thr Tyr Leu Leu Lys Trp Pro Lys Thr Phe Ile Val Val Ser

225 230 235 240

His Ala Arg Glu Phe Leu Asn Thr Val Val Thr Asp Ile Leu His Leu

245 250 255

His Gly Gln Lys Leu His Ala Tyr Lys Gly Asp Tyr Asp Thr Phe Glu

260 265 270

Arg Thr Arg Glu Glu His Leu Lys Asn Gln Gln Lys Ala Phe Glu Thr

275 280 285

Asn Glu Lys Ala Arg Ser His Met Gln Ala Phe Ile Asp Lys Phe Arg

290 295 300

Tyr Asn Ala Lys Arg Ala Ser Leu Val Gln Ser Arg Ile Lys Ala Leu

305 310 315 320

Glu Arg Met Glu His Val Asp Ala Val Val Ser Asp Pro Asp Tyr Lys

325 330 335

Phe Glu Phe Pro Thr Pro Asp Asp Arg Pro Gly Pro Pro Ile Ile Ser

340 345 350

Phe Ser Asp Ala Ser Phe Gly Tyr Pro Gly Gly Pro Thr Leu Phe Lys

355 360 365

Asn Leu Asn Phe Gly Ile Asp Leu Asp Ser Arg Ile Ala Met Val Gly

370 375 380

Pro Asn Gly Ile Gly Lys Ser Thr Ile Leu Lys Leu Ile Ser Gly Asp

385 390 395 400

Leu Gln Pro Thr Ser Gly Thr Val Phe Arg Ser Pro Lys Val Arg Met

405 410 415

Ala Val Phe Asn Gln His His Val Asp Gly Leu Asp Leu Thr Val Asn

420 425 430

Pro Leu Leu Tyr Met Met Arg Cys Tyr Pro Gly Val Pro Glu Gln Lys

435 440 445

Leu Arg Ala His Leu Gly Ser Phe Gly Val Thr Gly Asn Leu Ala Leu

450 455 460

Gln Pro Met Tyr Thr Leu Ser Gly Gly Gln Lys Ser Arg Val Ala Phe

465 470 475 480

Ala Lys Ile Thr Phe Lys Lys Pro His Ile Ile Leu Leu Asp Glu Pro

485 490 495

Ser Asn His Leu Asp Leu Asp Ala Val Glu Ala Leu Ile Gln Gly Leu

500 505 510

Leu Val Phe Gln Gly Gly Val Leu Met Val Ser His Asp Glu His Leu

515 520 525

Ile Thr Gly Ser Val Asp Glu Leu Trp Val Val Ser Glu Gly Arg Val

530 535 540

Ser Pro Phe Ala Gly Thr Phe Lys Asp Tyr Lys Lys Met Leu Lys Ser

545 550 555 560

<210> SEQ ID NO 131

<211> LENGTH: 704

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 131

gcacgagctt ccgatctgct aaggttcgta tagcagtgtt cagtcagcac catgttgatg 60

gactcgactt atcctcaaat cctcttctgt atatgatgcg ctgctatcct ggagttccag 120

aacagaagct tcgagctcac ttaggttctt ttggtgtaac cggaaatctt gcgctgcagc 180

caatgtatac tttgtcaggt ggtcagaaaa gcagggttgc ctttgcaaag ataactttta 240

agaagccaca cataatattg cttgatgagc catccaatca tctggatttg gatgctgttg 300

aggcgcttat tcaaggtctt gtgctgttcc aaggaggcat tctcatggtg agtcacgatg 360

agcacctcat ctctggaagc gtggaggaac tatgggttgt atccgaagga agagtggcac 420

cattccatgg cacattccaa gattataaga agatactcca atcatcctag aaatgtcaaa 480

ttttgtggat gtatactgga aagacccctg gatcacattt tttttgctgt tcctctcagc 540

tgtcatacaa ttttgcaatt tatccaaacc tatgtatttc aaggcaattt tatgatttta 600

tgaacacgag gttaaattaa ctattaaata attttgtaat aaccttataa ttataagatg 660

tataatttat actttcacta cttttcaaaa aaaaaaaaaa aaaa 704

<210> SEQ ID NO 132

<211> LENGTH: 155

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 132

Thr Ser Phe Arg Ser Ala Lys Val Arg Ile Ala Val Phe Ser Gln His

1 5 10 15

His Val Asp Gly Leu Asp Leu Ser Ser Asn Pro Leu Leu Tyr Met Met

20 25 30

Arg Cys Tyr Pro Gly Val Pro Glu Gln Lys Leu Arg Ala His Leu Gly

35 40 45

Ser Phe Gly Val Thr Gly Asn Leu Ala Leu Gln Pro Met Tyr Thr Leu

50 55 60

Ser Gly Gly Gln Lys Ser Arg Val Ala Phe Ala Lys Ile Thr Phe Lys

65 70 75 80

Lys Pro His Ile Ile Leu Leu Asp Glu Pro Ser Asn His Leu Asp Leu

85 90 95

Asp Ala Val Glu Ala Leu Ile Gln Gly Leu Val Leu Phe Gln Gly Gly

100 105 110

Ile Leu Met Val Ser His Asp Glu His Leu Ile Ser Gly Ser Val Glu

115 120 125

Glu Leu Trp Val Val Ser Glu Gly Arg Val Ala Pro Phe His Gly Thr

130 135 140

Phe Gln Asp Tyr Lys Lys Ile Leu Gln Ser Ser

145 150 155

<210> SEQ ID NO 133

<211> LENGTH: 767

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 133

gcacgagggg aagtccacta tactgaaatt aatatctgga gacctgcagc caacttcggg 60

aacagtgttt cgctccccca aggtccgcat ggctgtattc agtcagcatc atgttgatgg 120

›SEQUENCE LISTING · 34 of 53

acttgatctg acggtgaacc cccttctgta catgatgaga tgcttcccgg gtgtacctga 180

acagaaactg aggtcacatt tgggttcctt cggtgttaca ggaaatcttg ccctccaatc 240

tatgtacact ttatcaggtg gtcagaagag tagggtcgcg tttgcgaaga tcaccttcaa 300

gaagccgcac attatccttc ttgatgagcc ttctaaccat cttgatctcg acgcagtgga 360

ggcgctcatc cagggtctgc tcatattcca gggaggagtg ctgatggtga gtcacgacga 420

gcatctgatc acggggagtg tggacgagct ctgggcggtg acggacggca aggtcgcccc 480

gttcccgggc acgttcaagg agtacaagaa gatgctcacg acatgaacct ggaaaaagga 540

cgggacatag gggaagatgc ccacacagac ataaagaaac tatatgtatg atatgtacgc 600

cctggcatgg gaaactgatg taatcttatg cttgtcacct acctgaggtt tgaaagtttg 660

agtagaacta tgctactaca tacagtagta caattttgtt tgttccagcc tgcctgacaa 720

tctgcatcga gcttgcgatt ttcttggaca aaaaaaaaaa aaaaaaa 767

<210> SEQ ID NO 134

<211> LENGTH: 174

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 134

His Glu Gly Lys Ser Thr Ile Leu Lys Leu Ile Ser Gly Asp Leu Gln

1 5 10 15

Pro Thr Ser Gly Thr Val Phe Arg Ser Pro Lys Val Arg Met Ala Val

20 25 30

Phe Ser Gln His His Val Asp Gly Leu Asp Leu Thr Val Asn Pro Leu

35 40 45

Leu Tyr Met Met Arg Cys Phe Pro Gly Val Pro Glu Gln Lys Leu Arg

50 55 60

Ser His Leu Gly Ser Phe Gly Val Thr Gly Asn Leu Ala Leu Gln Ser

65 70 75 80

Met Tyr Thr Leu Ser Gly Gly Gln Lys Ser Arg Val Ala Phe Ala Lys

85 90 95

Ile Thr Phe Lys Lys Pro His Ile Ile Leu Leu Asp Glu Pro Ser Asn

100 105 110

His Leu Asp Leu Asp Ala Val Glu Ala Leu Ile Gln Gly Leu Leu Ile

115 120 125

Phe Gln Gly Gly Val Leu Met Val Ser His Asp Glu His Leu Ile Thr

130 135 140

Gly Ser Val Asp Glu Leu Trp Ala Val Thr Asp Gly Lys Val Ala Pro

145 150 155 160

Phe Pro Gly Thr Phe Lys Glu Tyr Lys Lys Met Leu Thr Thr

165 170

<210> SEQ ID NO 135

<211> LENGTH: 646

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 135

gcacgagcat cacccatcgt gcgtcgatag acgatgatgc cggagtcgca tccgcatccc 60

cacgcggggg cggtgctggc gtcgacgtct agccgtcgga gcctcagctt ggggtcctcc 120

atctcgcagt cgttccggca gatggacacg gaggacccct tcgcgcggtc gcagtcggag 180

catgagcacc gcgacgacga ggagaacctg cgctgggccg cgctcgagaa gctgcccacc 240

tacgaccgca tgcgccaggg tatcctacgc cgggcgctcg accaacaaca agagagcggc 300

ggcggcggcg tcgagatcgt cgacatccac aagctggccg ccggcgacgg gggccgtgcg 360

ctcctggagc gcctcttcca ggacgacagc gagcgattcc tgcgccggct cagggaccga 420

atcgacatgg ttggcatcga gctccccaca gtggaagttc ggtacgagca gttgacggtg 480

gaggctgacg tgattaccgc cgggagggcc ctcccgacgc tgtggaacgc cgccaccaat 540

tttcttcagg tttatcatag taattaatta attaatctct tttctattcc tttaaagcat 600

caattttgac ggtcttgttt ccctcaaaaa aaaaaaaaaa aaaaaa 646

<210> SEQ ID NO 136

<211> LENGTH: 177

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 136

Met Met Pro Glu Ser His Pro His Pro His Ala Gly Ala Val Leu Ala

1 5 10 15

Ser Thr Ser Ser Arg Arg Ser Leu Ser Leu Gly Ser Ser Ile Ser Gln

20 25 30

Ser Phe Arg Gln Met Asp Thr Glu Asp Pro Phe Ala Arg Ser Gln Ser

35 40 45

Glu His Glu His Arg Asp Asp Glu Glu Asn Leu Arg Trp Ala Ala Leu

50 55 60

Glu Lys Leu Pro Thr Tyr Asp Arg Met Arg Gln Gly Ile Leu Arg Arg

65 70 75 80

Ala Leu Asp Gln Gln Gln Glu Ser Gly Gly Gly Gly Val Glu Ile Val

85 90 95

Asp Ile His Lys Leu Ala Ala Gly Asp Gly Gly Arg Ala Leu Leu Glu

100 105 110

Arg Leu Phe Gln Asp Asp Ser Glu Arg Phe Leu Arg Arg Leu Arg Asp

115 120 125

Arg Ile Asp Met Val Gly Ile Glu Leu Pro Thr Val Glu Val Arg Tyr

130 135 140

Glu Gln Leu Thr Val Glu Ala Asp Val Ile Thr Ala Gly Arg Ala Leu

145 150 155 160

Pro Thr Leu Trp Asn Ala Ala Thr Asn Phe Leu Gln Val Tyr His Ser

165 170 175

Asn

<210> SEQ ID NO 137

<211> LENGTH: 2031

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 137

gcacgagggt tcttccattc aaccctcttg ctatgtcttt tgataatgtg aactactatg 60

ttgacatgcc tgcggaaatg aaacatcaag gagtgcagga tgacagactc caattgttgc 120

gtgaggttac aggatcattc aggcctggag tgctgacagc acttatgggt gtcagtggag 180

ctggaaagac tactcttatg gatgttttgg ctggaagaaa gactgggggg tatattgaag 240

gagacatcag aattgcaggc tatcccaaga accaagcaac attcgcaagg atttctggat 300

actgcgagca aaatgatata cattctcctc aggtcacagt aagggagtct ttgatatact 360

ctgccttttt gcgccttcct ggaaaaattg gagatcaaga aataactgat gacataaaga 420

tgcaatttgt ggatgaagtt atggaactag tggagctcga caatctgagg gatgccttag 480

ttgggctacc aggaatcaca gggctttcga cagagcaaag aaaaaggttg acaatagccg 540

tggagctcgt tgccaatcca tcaatcatat ttatggatga accaacatca gggcttgatg 600

caagagctgc agcaattgtc atgagaactg tgcggaacac agttgacact ggacggacag 660

ttgtttgcac aatccatcag ccaagcatcg acatctttga atcttttgat gagttgctat 720

tgttgaaaag aggaggccag gtgatctact ctgggaaatt gggtcgcaac tcccagaaaa 780

tggtggagta ttttgaggca attcctggag tgcctaaaat aaaagataag tacaatcctg 840

caacgtggat gctcgaggtc agttcagttg caactgaagt gcgactaaag atggattttg 900

ctaagtacta tgaaacatca gatctgtata agcaaaacaa ggtactggtg aaccagctga 960

gtcaaccaga gccaggaaca tcagatcttt atttccctac agagtactcg caatccacaa 1020

taggacagtt caaagcctgt ctctggaagc aatggctgac ttattggcgc agcccggatt 1080

acaaccttgt tagatattcc ttcactctgt tagttgcctt gcttctcggc tccattttct 1140

ggaggatagg caccaacatg gaagatgcaa ccactctcgg tatggttatt ggagcaatgt 1200

acactgctgt tatgtttatc ggcatcaaca attgttcaac tgtgcagcca gttgtttcaa 1260

ttgaaagaac ggtcttctac agagagaggg ctgctgggat gtactctgct atgccatatg 1320

ccattgctca ggttgttata gagataccat atgtatttgt gcaaacaacg tactacaccc 1380

tcattgtata cgccatgatg agcttccaat ggacagctgt caagttcttc tggttcttct 1440

tcatttccta cttctccttc ctctacttca cctactatgg catgatggct gtctcaatct 1500

ccccaaacca tgaggttgca tccatcttcg cagcagcttt cttttctctc ttcaatctct 1560

tctcaggctt cttcattccg agaccgagaa ttcctggatg gtggatctgg tactactgga 1620

tttgcccact ggcatggaca gtgtatgggc tcatcgtgac acaatacgga gacctggaag 1680

acctcatctc agtccctgga gaaagtgagc agacgataag ctactatgta acacatcatt 1740

ttggatacca cagggatttt ctgccggtca ttgcgccggt tctggtactc tttgcagtat 1800

tcttcgcgtt cttgtacgca gtctgcatca agaaactgaa cttccagcaa cgataagaac 1860

aagatatggc agcaaaagct cttgagagtt tttttggaac attgccgtgt atatagggta 1920

ttttgcacat cgcgttctcc tttcattttt tgagtccctt taatacattg tcgtgtatat 1980

agggtattct gcacatcgcc aaatgtggtg aggtcttata cagatttcta g 2031

<210> SEQ ID NO 138

<211> LENGTH: 617

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 138

Thr Arg Val Leu Pro Phe Asn Pro Leu Ala Met Ser Phe Asp Asn Val

1 5 10 15

Asn Tyr Tyr Val Asp Met Pro Ala Glu Met Lys His Gln Gly Val Gln

›SEQUENCE LISTING · 35 of 53

20 25 30

Asp Asp Arg Leu Gln Leu Leu Arg Glu Val Thr Gly Ser Phe Arg Pro

35 40 45

Gly Val Leu Thr Ala Leu Met Gly Val Ser Gly Ala Gly Lys Thr Thr

50 55 60

Leu Met Asp Val Leu Ala Gly Arg Lys Thr Gly Gly Tyr Ile Glu Gly

65 70 75 80

Asp Ile Arg Ile Ala Gly Tyr Pro Lys Asn Gln Ala Thr Phe Ala Arg

85 90 95

Ile Ser Gly Tyr Cys Glu Gln Asn Asp Ile His Ser Pro Gln Val Thr

100 105 110

Val Arg Glu Ser Leu Ile Tyr Ser Ala Phe Leu Arg Leu Pro Gly Lys

115 120 125

Ile Gly Asp Gln Glu Ile Thr Asp Asp Ile Lys Met Gln Phe Val Asp

130 135 140

Glu Val Met Glu Leu Val Glu Leu Asp Asn Leu Arg Asp Ala Leu Val

145 150 155 160

Gly Leu Pro Gly Ile Thr Gly Leu Ser Thr Glu Gln Arg Lys Arg Leu

165 170 175

Thr Ile Ala Val Glu Leu Val Ala Asn Pro Ser Ile Ile Phe Met Asp

180 185 190

Glu Pro Thr Ser Gly Leu Asp Ala Arg Ala Ala Ala Ile Val Met Arg

195 200 205

Thr Val Arg Asn Thr Val Asp Thr Gly Arg Thr Val Val Cys Thr Ile

210 215 220

His Gln Pro Ser Ile Asp Ile Phe Glu Ser Phe Asp Glu Leu Leu Leu

225 230 235 240

Leu Lys Arg Gly Gly Gln Val Ile Tyr Ser Gly Lys Leu Gly Arg Asn

245 250 255

Ser Gln Lys Met Val Glu Tyr Phe Glu Ala Ile Pro Gly Val Pro Lys

260 265 270

Ile Lys Asp Lys Tyr Asn Pro Ala Thr Trp Met Leu Glu Val Ser Ser

275 280 285

Val Ala Thr Glu Val Arg Leu Lys Met Asp Phe Ala Lys Tyr Tyr Glu

290 295 300

Thr Ser Asp Leu Tyr Lys Gln Asn Lys Val Leu Val Asn Gln Leu Ser

305 310 315 320

Gln Pro Glu Pro Gly Thr Ser Asp Leu Tyr Phe Pro Thr Glu Tyr Ser

325 330 335

Gln Ser Thr Ile Gly Gln Phe Lys Ala Cys Leu Trp Lys Gln Trp Leu

340 345 350

Thr Tyr Trp Arg Ser Pro Asp Tyr Asn Leu Val Arg Tyr Ser Phe Thr

355 360 365

Leu Leu Val Ala Leu Leu Leu Gly Ser Ile Phe Trp Arg Ile Gly Thr

370 375 380

Asn Met Glu Asp Ala Thr Thr Leu Gly Met Val Ile Gly Ala Met Tyr

385 390 395 400

Thr Ala Val Met Phe Ile Gly Ile Asn Asn Cys Ser Thr Val Gln Pro

405 410 415

Val Val Ser Ile Glu Arg Thr Val Phe Tyr Arg Glu Arg Ala Ala Gly

420 425 430

Met Tyr Ser Ala Met Pro Tyr Ala Ile Ala Gln Val Val Ile Glu Ile

435 440 445

Pro Tyr Val Phe Val Gln Thr Thr Tyr Tyr Thr Leu Ile Val Tyr Ala

450 455 460

Met Met Ser Phe Gln Trp Thr Ala Val Lys Phe Phe Trp Phe Phe Phe

465 470 475 480

Ile Ser Tyr Phe Ser Phe Leu Tyr Phe Thr Tyr Tyr Gly Met Met Ala

485 490 495

Val Ser Ile Ser Pro Asn His Glu Val Ala Ser Ile Phe Ala Ala Ala

500 505 510

Phe Phe Ser Leu Phe Asn Leu Phe Ser Gly Phe Phe Ile Pro Arg Pro

515 520 525

Arg Ile Pro Gly Trp Trp Ile Trp Tyr Tyr Trp Ile Cys Pro Leu Ala

530 535 540

Trp Thr Val Tyr Gly Leu Ile Val Thr Gln Tyr Gly Asp Leu Glu Asp

545 550 555 560

Leu Ile Ser Val Pro Gly Glu Ser Glu Gln Thr Ile Ser Tyr Tyr Val

565 570 575

Thr His His Phe Gly Tyr His Arg Asp Phe Leu Pro Val Ile Ala Pro

580 585 590

Val Leu Val Leu Phe Ala Val Phe Phe Ala Phe Leu Tyr Ala Val Cys

595 600 605

Ile Lys Lys Leu Asn Phe Gln Gln Arg

610 615

<210> SEQ ID NO 139

<211> LENGTH: 4159

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 139

gcacgagtgt atcccggatg acgctgctgc tgggcccgcc gtcgtcgggg aagacgaccc 60

tcctcctggc cctcgccggg aagctggacc cgtcgctccg gcgcggcggc gaggtgacgt 120

acaacgggtt cgagctggag gagttcgtgg cgcagaagac ggcggcgtac atcagccaga 180

cggacgtgca cgtcggcgag atgaccgtca aggagacgct cgacttctcg gccaggtgcc 240

agggcgtcgg caccaagtac gatcttctga cagagctggc aaggagggag aaggaggccg 300

gcatccggcc ggagcccgag gtcgacctct tcatgaaggc tacttcgatg gaaggagtcg 360

agagcagcct tcagacagat tacaccctca gaatactggg attggatata tgcgcggaca 420

cgatcgtcgg cgaccagatg cagaggggga tctccggtgg tcagaagaaa cgcgtcacca 480

ccggtgagat gattgtcggt ccaacaaagg ttctattcat ggatgagata tcaactggat 540

tggacagctc caccacattc cagattgtca aatgccttca gcaaatcgtg cacttgggcg 600

aggcaaccat cctcatgtca ctcctacaac cagcccctga gacttttgag ctattcgatg 660

acattatcct actgtcagaa ggccagattg tttatcaggg accccgcgaa tacgtccttg 720

agttctttga gtcatgcgga ttccgctgcc cagagcgtaa gggtactgca gactttcttc 780

aggaggtgac atcaaagaag gatcaggagc agtattgggc cgacaagcat aggccataca 840

gatacatttc agtctcagaa tttgcacaga gatttaaaag gttccatgtt gggctccaac 900

ttgagaatca tctctcagtc ccatttgata aaactcgtag ccatcaggct gctcttgtct 960

tctcgaagca atcggtgtca acaacagagc tcctcaaggc atcctttgcc aaggagtggc 1020

tcctcattaa gcgcaactca tttgtgtaca tcttcaagac catacagctc atcattgtag 1080

cccttgtcgc gtcgacagtg tttcttagga cccagatgca cacaaggaat ttagatgatg 1140

gctttgtgta cattggagca ctacttttca gtctgattgt gaacatgttc aatggtttcg 1200

ctgagctctc tttgaccatc acaaggttgc cagtgttctt caagcaccgg gacctcctct 1260

tctaccctgc ttggatcttc actctaccga atgttattct gagaatccca ttttccatca 1320

tcgaatctat agtctgggtg attgttacat actacactat aggatttgcc ccagaggctg 1380

acagattttt caagcagttg ctgctagtgt tcttgatcca gcagatggca ggtggccttt 1440

tcagagcaac tgctggtctg tgcagatcca tgatcattgc tcaaactgga ggagccctgg 1500

cccttctcat atttttcgtt cttggaggat ttcttctgcc aaaagcattc atcccaaaat 1560

ggtggatctg gggttactgg gtttcaccac tgatgtacgg gtataatgct ctagcagtca 1620

atgaattcta ctctcctcgg tggatgaaca agtttgtact ggataacaat ggcgttccta 1680

aaagactagg aatagctctg atggaaggtg ccaacatctt cactgacaaa aattggttct 1740

ggattggagc agcagggctc ctgggtttca ccatgttttt caatgtgctt ttcactctgt 1800

cactcgttta tctgaatcct ctgggcaaac cacaagctgt catatctgaa gaaactgcga 1860

aggaagcgga aggcaatggg gatgcaagac atacagtaag aaatggcagc acaaaatcaa 1920

atggtggaaa tcacaaagaa atgagggaga tgagattgag tgctcgtctg agcaatagtt 1980

catcgaatgg agtttcacga ctgatgtcca ttggtagcaa tgaagctggt ccaagaagag 2040

gaatggttct tccatttact cctctatcca tgtcttttga tgatgtgaac tactatgtcg 2100

acatgcctgc agaaatgaag cagcaaggag tggtggatga taggctccaa ttgttacgtg 2160

acgttactgg atcatttagg cctgcagtgc tgacagcact catgggagtc agtggagccg 2220

gaaagacaac tcttatggat gttttggcag gaagaaagac tggtggttac attgaaggag 2280

atatgagaat ttctggttat cctaagaacc aagaaacatt tgcaagaatt tctggctact 2340

gtgagcaaaa cgatatccat tcacctcagg tcacagttag ggagtctttg atatactctg 2400

ctttcctgcg ccttccagaa aaaataggag atcaagaaat cactgatgat atcaagattc 2460

aatttgttga tgaagttatg gaactagtgg agctcgacaa tctgaaggat gcgttagttg 2520

gcctgcctgg aatcacaggg ctttcaacag agcaaaggaa gagattgaca atagcagtgg 2580

agcttgttgc aaatccctcg atcatcttca tggatgaacc gacttcaggt cttgatgcaa 2640

gagcagcagc cattgttatg agaacagtga ggaacacagt tgacactgga cggacagtgg 2700

tttgcacaat tcaccagcca agcattgaca tatttgaggc ttttgatgag ctgctactac 2760

›SEQUENCE LISTING · 36 of 53

tgaaaagagg agggcaggtg atatactctg ggcaattggg tcgtaattcc cagaaaatga 2820

ttgaatattt cgaggcaatt cctggtgtgc ctaaaatcaa agataagtac aatccagcta 2880

catggatgct tgaggtcagt tcagttgctg cggaagtacg cttaaatatg gactttgctg 2940

agtactataa gacatcagac ctgtacaagc aaaacaaggt attggtgaat cagctaagtc 3000

aaccagagcc aggaacatca gatctgcatt ttcctacaaa atactctcaa tccaccatag 3060

ggcaatttag ggcctgcctc tggaagcaat ggctgaccta ttggcgcagc ccagattaca 3120

atcttgttag attttccttc actctgttca cagccttgct actcggcacc atcttttgga 3180

agatcggcac caagatggga aatgccaatt ctcttagaat ggtcattgga gcaatgtata 3240

cagcagtgat gtttatcggt atcaacaatt gtgcgactgt gcagccaatc gtgtcgattg 3300

agagaacagt tttctaccga gagagggctg ctgggatgta ctctgctatg ccctatgcca 3360

ttgctcaggt tgtcatggag ataccctatg tgttcgtcca aactgcatat tataccctca 3420

ttgtttatgc catgatgagc ttccagtgga cagcagccaa gttcttctgg ttcttcttcg 3480

tctcctactt ctcatttctc tacttcacct actatggtat gatgacggtg gcaatctcac 3540

caaaccatga ggttgcagcc atctttgccg cggcgttcta ttccttgttc aacctattct 3600

caagattctt cattccgaga ccaaggattc ccaaatggtg gatctggtac tactggcttt 3660

gcccattggc atggacagtg tatgggctca tagtgacaca gtatggagac ctagaacaaa 3720

tcatctcagt ccctggccaa tccaaccaga caatcagcta ctatgttact catcattttg 3780

gatatcacag gaaatttatg ccagttgttg cgccggtgct cgtgctcttc gctgtgtttt 3840

tcgcgttcat gtatgccatt tgcatcaaga agttgaactt ccaacatcga taggggcatt 3900

gaaacaacag gcaaaatctg gttggaaact tttttttggt tttgaaccaa atcttctttt 3960

gtcctttccc cacagataat atcttctttt atcctcacat ttttgtggtc tatatatgcc 4020

atgtaaatat gctggtaggt tttctgtatt ccatacatcg ccaaatgtgg cgaggtttca 4080

tatgaccttt tagctttgga aatgtaaact ttataagagc attgttgtca gaaattttgt 4140

taaaaaaaaa aaaaaaaaa 4159

<210> SEQ ID NO 140

<211> LENGTH: 1296

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 140

Thr Ser Val Ser Arg Met Thr Leu Leu Leu Gly Pro Pro Ser Ser Gly

1 5 10 15

Lys Thr Thr Leu Leu Leu Ala Leu Ala Gly Lys Leu Asp Pro Ser Leu

20 25 30

Arg Arg Gly Gly Glu Val Thr Tyr Asn Gly Phe Glu Leu Glu Glu Phe

35 40 45

Val Ala Gln Lys Thr Ala Ala Tyr Ile Ser Gln Thr Asp Val His Val

50 55 60

Gly Glu Met Thr Val Lys Glu Thr Leu Asp Phe Ser Ala Arg Cys Gln

65 70 75 80

Gly Val Gly Thr Lys Tyr Asp Leu Leu Thr Glu Leu Ala Arg Arg Glu

85 90 95

Lys Glu Ala Gly Ile Arg Pro Glu Pro Glu Val Asp Leu Phe Met Lys

100 105 110

Ala Thr Ser Met Glu Gly Val Glu Ser Ser Leu Gln Thr Asp Tyr Thr

115 120 125

Leu Arg Ile Leu Gly Leu Asp Ile Cys Ala Asp Thr Ile Val Gly Asp

130 135 140

Gln Met Gln Arg Gly Ile Ser Gly Gly Gln Lys Lys Arg Val Thr Thr

145 150 155 160

Gly Glu Met Ile Val Gly Pro Thr Lys Val Leu Phe Met Asp Glu Ile

165 170 175

Ser Thr Gly Leu Asp Ser Ser Thr Thr Phe Gln Ile Val Lys Cys Leu

180 185 190

Gln Gln Ile Val His Leu Gly Glu Ala Thr Ile Leu Met Ser Leu Leu

195 200 205

Gln Pro Ala Pro Glu Thr Phe Glu Leu Phe Asp Asp Ile Ile Leu Leu

210 215 220

Ser Glu Gly Gln Ile Val Tyr Gln Gly Pro Arg Glu Tyr Val Leu Glu

225 230 235 240

Phe Phe Glu Ser Cys Gly Phe Arg Cys Pro Glu Arg Lys Gly Thr Ala

245 250 255

Asp Phe Leu Gln Glu Val Thr Ser Lys Lys Asp Gln Glu Gln Tyr Trp

260 265 270

Ala Asp Lys His Arg Pro Tyr Arg Tyr Ile Ser Val Ser Glu Phe Ala

275 280 285

Gln Arg Phe Lys Arg Phe His Val Gly Leu Gln Leu Glu Asn His Leu

290 295 300

Ser Val Pro Phe Asp Lys Thr Arg Ser His Gln Ala Ala Leu Val Phe

305 310 315 320

Ser Lys Gln Ser Val Ser Thr Thr Glu Leu Leu Lys Ala Ser Phe Ala

325 330 335

Lys Glu Trp Leu Leu Ile Lys Arg Asn Ser Phe Val Tyr Ile Phe Lys

340 345 350

Thr Ile Gln Leu Ile Ile Val Ala Leu Val Ala Ser Thr Val Phe Leu

355 360 365

Arg Thr Gln Met His Thr Arg Asn Leu Asp Asp Gly Phe Val Tyr Ile

370 375 380

Gly Ala Leu Leu Phe Ser Leu Ile Val Asn Met Phe Asn Gly Phe Ala

385 390 395 400

Glu Leu Ser Leu Thr Ile Thr Arg Leu Pro Val Phe Phe Lys His Arg

405 410 415

Asp Leu Leu Phe Tyr Pro Ala Trp Ile Phe Thr Leu Pro Asn Val Ile

420 425 430

Leu Arg Ile Pro Phe Ser Ile Ile Glu Ser Ile Val Trp Val Ile Val

435 440 445

Thr Tyr Tyr Thr Ile Gly Phe Ala Pro Glu Ala Asp Arg Phe Phe Lys

450 455 460

Gln Leu Leu Leu Val Phe Leu Ile Gln Gln Met Ala Gly Gly Leu Phe

465 470 475 480

Arg Ala Thr Ala Gly Leu Cys Arg Ser Met Ile Ile Ala Gln Thr Gly

485 490 495

Gly Ala Leu Ala Leu Leu Ile Phe Phe Val Leu Gly Gly Phe Leu Leu

500 505 510

Pro Lys Ala Phe Ile Pro Lys Trp Trp Ile Trp Gly Tyr Trp Val Ser

515 520 525

Pro Leu Met Tyr Gly Tyr Asn Ala Leu Ala Val Asn Glu Phe Tyr Ser

530 535 540

Pro Arg Trp Met Asn Lys Phe Val Leu Asp Asn Asn Gly Val Pro Lys

545 550 555 560

Arg Leu Gly Ile Ala Leu Met Glu Gly Ala Asn Ile Phe Thr Asp Lys

565 570 575

Asn Trp Phe Trp Ile Gly Ala Ala Gly Leu Leu Gly Phe Thr Met Phe

580 585 590

Phe Asn Val Leu Phe Thr Leu Ser Leu Val Tyr Leu Asn Pro Leu Gly

595 600 605

Lys Pro Gln Ala Val Ile Ser Glu Glu Thr Ala Lys Glu Ala Glu Gly

610 615 620

Asn Gly Asp Ala Arg His Thr Val Arg Asn Gly Ser Thr Lys Ser Asn

625 630 635 640

Gly Gly Asn His Lys Glu Met Arg Glu Met Arg Leu Ser Ala Arg Leu

645 650 655

Ser Asn Ser Ser Ser Asn Gly Val Ser Arg Leu Met Ser Ile Gly Ser

660 665 670

Asn Glu Ala Gly Pro Arg Arg Gly Met Val Leu Pro Phe Thr Pro Leu

675 680 685

Ser Met Ser Phe Asp Asp Val Asn Tyr Tyr Val Asp Met Pro Ala Glu

690 695 700

Met Lys Gln Gln Gly Val Val Asp Asp Arg Leu Gln Leu Leu Arg Asp

705 710 715 720

Val Thr Gly Ser Phe Arg Pro Ala Val Leu Thr Ala Leu Met Gly Val

725 730 735

Ser Gly Ala Gly Lys Thr Thr Leu Met Asp Val Leu Ala Gly Arg Lys

740 745 750

Thr Gly Gly Tyr Ile Glu Gly Asp Met Arg Ile Ser Gly Tyr Pro Lys

755 760 765

Asn Gln Glu Thr Phe Ala Arg Ile Ser Gly Tyr Cys Glu Gln Asn Asp

770 775 780

Ile His Ser Pro Gln Val Thr Val Arg Glu Ser Leu Ile Tyr Ser Ala

785 790 795 800

Phe Leu Arg Leu Pro Glu Lys Ile Gly Asp Gln Glu Ile Thr Asp Asp

805 810 815

Ile Lys Ile Gln Phe Val Asp Glu Val Met Glu Leu Val Glu Leu Asp

820 825 830

Asn Leu Lys Asp Ala Leu Val Gly Leu Pro Gly Ile Thr Gly Leu Ser

835 840 845

Thr Glu Gln Arg Lys Arg Leu Thr Ile Ala Val Glu Leu Val Ala Asn

850 855 860

Pro Ser Ile Ile Phe Met Asp Glu Pro Thr Ser Gly Leu Asp Ala Arg

865 870 875 880

Ala Ala Ala Ile Val Met Arg Thr Val Arg Asn Thr Val Asp Thr Gly

885 890 895

Arg Thr Val Val Cys Thr Ile His Gln Pro Ser Ile Asp Ile Phe Glu

900 905 910

Ala Phe Asp Glu Leu Leu Leu Leu Lys Arg Gly Gly Gln Val Ile Tyr

›SEQUENCE LISTING · 37 of 53

915 920 925

Ser Gly Gln Leu Gly Arg Asn Ser Gln Lys Met Ile Glu Tyr Phe Glu

930 935 940

Ala Ile Pro Gly Val Pro Lys Ile Lys Asp Lys Tyr Asn Pro Ala Thr

945 950 955 960

Trp Met Leu Glu Val Ser Ser Val Ala Ala Glu Val Arg Leu Asn Met

965 970 975

Asp Phe Ala Glu Tyr Tyr Lys Thr Ser Asp Leu Tyr Lys Gln Asn Lys

980 985 990

Val Leu Val Asn Gln Leu Ser Gln Pro Glu Pro Gly Thr Ser Asp Leu

995 1000 1005

His Phe Pro Thr Lys Tyr Ser Gln Ser Thr Ile Gly Gln Phe Arg Ala

1010 1015 1020

Cys Leu Trp Lys Gln Trp Leu Thr Tyr Trp Arg Ser Pro Asp Tyr Asn

1025 1030 1035 1040

Leu Val Arg Phe Ser Phe Thr Leu Phe Thr Ala Leu Leu Leu Gly Thr

1045 1050 1055

Ile Phe Trp Lys Ile Gly Thr Lys Met Gly Asn Ala Asn Ser Leu Arg

1060 1065 1070

Met Val Ile Gly Ala Met Tyr Thr Ala Val Met Phe Ile Gly Ile Asn

1075 1080 1085

Asn Cys Ala Thr Val Gln Pro Ile Val Ser Ile Glu Arg Thr Val Phe

1090 1095 1100

Tyr Arg Glu Arg Ala Ala Gly Met Tyr Ser Ala Met Pro Tyr Ala Ile

1105 1110 1115 1120

Ala Gln Val Val Met Glu Ile Pro Tyr Val Phe Val Gln Thr Ala Tyr

1125 1130 1135

Tyr Thr Leu Ile Val Tyr Ala Met Met Ser Phe Gln Trp Thr Ala Ala

1140 1145 1150

Lys Phe Phe Trp Phe Phe Phe Val Ser Tyr Phe Ser Phe Leu Tyr Phe

1155 1160 1165

Thr Tyr Tyr Gly Met Met Thr Val Ala Ile Ser Pro Asn His Glu Val

1170 1175 1180

Ala Ala Ile Phe Ala Ala Ala Phe Tyr Ser Leu Phe Asn Leu Phe Ser

1185 1190 1195 1200

Arg Phe Phe Ile Pro Arg Pro Arg Ile Pro Lys Trp Trp Ile Trp Tyr

1205 1210 1215

Tyr Trp Leu Cys Pro Leu Ala Trp Thr Val Tyr Gly Leu Ile Val Thr

1220 1225 1230

Gln Tyr Gly Asp Leu Glu Gln Ile Ile Ser Val Pro Gly Gln Ser Asn

1235 1240 1245

Gln Thr Ile Ser Tyr Tyr Val Thr His His Phe Gly Tyr His Arg Lys

1250 1255 1260

Phe Met Pro Val Val Ala Pro Val Leu Val Leu Phe Ala Val Phe Phe

1265 1270 1275 1280

Ala Phe Met Tyr Ala Ile Cys Ile Lys Lys Leu Asn Phe Gln His Arg

1285 1290 1295

<210> SEQ ID NO 141

<211> LENGTH: 509

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 141

gcacgaggaa acaaggtgga tctgaatgga gggaagtgat atatacagag caagtaacag 60

tttacgaaga agttccacgg tttggagaaa cagtggtgtg gaggtattct caaggtcttc 120

tcgcgaagag gatgacgaag aagctctgaa atgggctgca cttgagaagc tccctaccta 180

caaccgtctc aggaaaggct tgctcacagc ttcccatggc gtcgccaacg aaatcgacgt 240

ctctgatctc ggcatccaag agagacagaa acttctcgag aggttggtca aagtagctga 300

agaggacaat gagaggttcc tgttgaagct caaggaacgt attgatagag ttggacttga 360

tattccaaca attgaagttc gatatgagca ccttaatatt gaggcagagg cctttgtggg 420

aagtagagct ttgccctctt tcatcaactc tgttactaat gtcgtagagg gatttttcaa 480

tcttctccac attagcacaa gcaaaaaaa 509

<210> SEQ ID NO 142

<211> LENGTH: 161

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 142

Met Glu Gly Ser Asp Ile Tyr Arg Ala Ser Asn Ser Leu Arg Arg Ser

1 5 10 15

Ser Thr Val Trp Arg Asn Ser Gly Val Glu Val Phe Ser Arg Ser Ser

20 25 30

Arg Glu Glu Asp Asp Glu Glu Ala Leu Lys Trp Ala Ala Leu Glu Lys

35 40 45

Leu Pro Thr Tyr Asn Arg Leu Arg Lys Gly Leu Leu Thr Ala Ser His

50 55 60

Gly Val Ala Asn Glu Ile Asp Val Ser Asp Leu Gly Ile Gln Glu Arg

65 70 75 80

Gln Lys Leu Leu Glu Arg Leu Val Lys Val Ala Glu Glu Asp Asn Glu

85 90 95

Arg Phe Leu Leu Lys Leu Lys Glu Arg Ile Asp Arg Val Gly Leu Asp

100 105 110

Ile Pro Thr Ile Glu Val Arg Tyr Glu His Leu Asn Ile Glu Ala Glu

115 120 125

Ala Phe Val Gly Ser Arg Ala Leu Pro Ser Phe Ile Asn Ser Val Thr

130 135 140

Asn Val Val Glu Gly Phe Phe Asn Leu Leu His Ile Ser Thr Ser Lys

145 150 155 160

Lys

<210> SEQ ID NO 143

<211> LENGTH: 1977

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 143

gcacgaggga ggatacatag agggggaaat caccgtatca ggctatccaa agaagcaaga 60

gacctttgca cgtatatcgg gatactgtga gcaaaatgat attcattctc cacatgtgac 120

aatatatgaa tcactcgtat tttctgcatg gctgcggctt cctgcagagg ttgactcaga 180

aagaagaaag atgttcatcg aggagatcat ggatcttgta gagctcacat cattgagggg 240

ggcacttgtt gggctccctg gagtgaatgg tctatcaact gagcaacgca agaggcttac 300

aattgccgtg gagcttgttg ctaacccgtc gatcattttt atggatgagc caacatctgg 360

tcttgatgct cgtgcagctg caattgtgat gaggactgtt aggaacactg ttaacactgg 420

caggaccgtt gtttgcacca tccaccagcc aagtattgac atatttgaag catttgatga 480

gcttttcttg atgaagagag ggggagaaga aatttatgtt ggtcctgttg gccaaaattc 540

agcaaatttg attgagtatt ttgaggaaat tgaaggcatt agtaagataa aagatggata 600

taacccagcg acatggatgt tggaagtgtc ctctagtgcg caggaggaaa tgcttggtat 660

tgatttcgct gaagtttaca gacaatcaga actatatcaa aggaacaagg aactcataaa 720

ggagctaagc atgccagctc ctggctctag tgatctcaac ttccctacac agtactctag 780

atcattcgtc acacagtgtc tagcttgctt gtggaagcaa agttgtcata ttggagaaac 840

ccatcctata cggcagtgag attactcttc accatcgtca ttgcgctaat gttcggaacg 900

atgttctggg accttggaag taaaactcga agatcacagg atctattcaa tgccatggga 960

tcaatgtatg ctgcagtact atacatcgga gtgcaaaatt ctggttctgt tcaaccagtt 1020

gtcgtggtgg agcgaacagt cttttaccgg gaaagagcag ctggcatgta ttcagctttt 1080

ccatatgcat ttggacaggt tgctatcgaa tttccgtacg tattggttca ggctttgata 1140

tatggcgggc tagtctattc catgattgga ttcgaatgga cagttgccaa gttcttgtgg 1200

tacttgttct tcatgtactt caccatgcta tatttcacgt tctatggaat gatggctgtt 1260

ggcttgacgc cgaatgagag catagcagct atcatctcat cagcattcta caacgtatgg 1320

aacctcttct caggatatct tatcccccga cctaaacttc ctatttggtg gaggtggtac 1380

tcatggattt gcccggtggc atggacgcta tacggactgg ttgcctccca gtttggtgat 1440

atccaacaac cgctcgatca gggagttcca ggcccacaga taactgtggc ccagtttgtc 1500

acggactact ttggtttcca tcatgacttc ttgtgggttg tggcgatggt gcacgttgct 1560

ttcaccgtgc tgttcgcctt cctgtttagt ttcgccatca tgaggttcaa cttccagaaa 1620

agatgaggag gatgacattc tttcagattc cttgtacgta gtgccatact gctgtccaac 1680

agccagtgcg gatggattga acaaagtata gttgtttctg tagatgatat agaggttatt 1740

ttgtatttca agcatgtaga aattccgcac aactatgctc tctactctct atgacttgta 1800

ggaaaggcta attagtcttg ttgagcctcc agcttcttta ccagctctag ctgagtaact 1860

gctacttact tttctcgtcg gagaatgtaa acccgaccag taatgttgtc atttctgacg 1920

agttaatgga ggcagaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 1977

<210> SEQ ID NO 144

<211> LENGTH: 539

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (272)..(273)

<400> SEQUENCE: 144

Gly Gly Tyr Ile Glu Gly Glu Ile Thr Val Ser Gly Tyr Pro Lys Lys

1 5 10 15

Gln Glu Thr Phe Ala Arg Ile Ser Gly Tyr Cys Glu Gln Asn Asp Ile

›SEQUENCE LISTING · 38 of 53

20 25 30

His Ser Pro His Val Thr Ile Tyr Glu Ser Leu Val Phe Ser Ala Trp

35 40 45

Leu Arg Leu Pro Ala Glu Val Asp Ser Glu Arg Arg Lys Met Phe Ile

50 55 60

Glu Glu Ile Met Asp Leu Val Glu Leu Thr Ser Leu Arg Gly Ala Leu

65 70 75 80

Val Gly Leu Pro Gly Val Asn Gly Leu Ser Thr Glu Gln Arg Lys Arg

85 90 95

Leu Thr Ile Ala Val Glu Leu Val Ala Asn Pro Ser Ile Ile Phe Met

100 105 110

Asp Glu Pro Thr Ser Gly Leu Asp Ala Arg Ala Ala Ala Ile Val Met

115 120 125

Arg Thr Val Arg Asn Thr Val Asn Thr Gly Arg Thr Val Val Cys Thr

130 135 140

Ile His Gln Pro Ser Ile Asp Ile Phe Glu Ala Phe Asp Glu Leu Phe

145 150 155 160

Leu Met Lys Arg Gly Gly Glu Glu Ile Tyr Val Gly Pro Val Gly Gln

165 170 175

Asn Ser Ala Asn Leu Ile Glu Tyr Phe Glu Glu Ile Glu Gly Ile Ser

180 185 190

Lys Ile Lys Asp Gly Tyr Asn Pro Ala Thr Trp Met Leu Glu Val Ser

195 200 205

Ser Ser Ala Gln Glu Glu Met Leu Gly Ile Asp Phe Ala Glu Val Tyr

210 215 220

Arg Gln Ser Glu Leu Tyr Gln Arg Asn Lys Glu Leu Ile Lys Glu Leu

225 230 235 240

Ser Met Pro Ala Pro Gly Ser Ser Asp Leu Asn Phe Pro Thr Gln Tyr

245 250 255

Ser Arg Ser Phe Val Thr Gln Cys Leu Ala Cys Leu Trp Lys Gln Xaa

260 265 270

Xaa Ser Tyr Trp Arg Asn Pro Ser Tyr Thr Ala Val Arg Leu Leu Phe

275 280 285

Thr Ile Val Ile Ala Leu Met Phe Gly Thr Met Phe Trp Asp Leu Gly

290 295 300

Ser Lys Thr Arg Arg Ser Gln Asp Leu Phe Asn Ala Met Gly Ser Met

305 310 315 320

Tyr Ala Ala Val Leu Tyr Ile Gly Val Gln Asn Ser Gly Ser Val Gln

325 330 335

Pro Val Val Val Val Glu Arg Thr Val Phe Tyr Arg Glu Arg Ala Ala

340 345 350

Gly Met Tyr Ser Ala Phe Pro Tyr Ala Phe Gly Gln Val Ala Ile Glu

355 360 365

Phe Pro Tyr Val Leu Val Gln Ala Leu Ile Tyr Gly Gly Leu Val Tyr

370 375 380

Ser Met Ile Gly Phe Glu Trp Thr Val Ala Lys Phe Leu Trp Tyr Leu

385 390 395 400

Phe Phe Met Tyr Phe Thr Met Leu Tyr Phe Thr Phe Tyr Gly Met Met

405 410 415

Ala Val Gly Leu Thr Pro Asn Glu Ser Ile Ala Ala Ile Ile Ser Ser

420 425 430

Ala Phe Tyr Asn Val Trp Asn Leu Phe Ser Gly Tyr Leu Ile Pro Arg

435 440 445

Pro Lys Leu Pro Ile Trp Trp Arg Trp Tyr Ser Trp Ile Cys Pro Val

450 455 460

Ala Trp Thr Leu Tyr Gly Leu Val Ala Ser Gln Phe Gly Asp Ile Gln

465 470 475 480

Gln Pro Leu Asp Gln Gly Val Pro Gly Pro Gln Ile Thr Val Ala Gln

485 490 495

Phe Val Thr Asp Tyr Phe Gly Phe His His Asp Phe Leu Trp Val Val

500 505 510

Ala Met Val His Val Ala Phe Thr Val Leu Phe Ala Phe Leu Phe Ser

515 520 525

Phe Ala Ile Met Arg Phe Asn Phe Gln Lys Arg

530 535

<210> SEQ ID NO 145

<211> LENGTH: 549

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (2)

<221> NAME/KEY: unsure

<222> LOCATION: (12)

<221> NAME/KEY: unsure

<222> LOCATION: (15)

<221> NAME/KEY: unsure

<222> LOCATION: (20)

<221> NAME/KEY: unsure

<222> LOCATION: (23)

<221> NAME/KEY: unsure

<222> LOCATION: (31)

<221> NAME/KEY: unsure

<222> LOCATION: (39)

<221> NAME/KEY: unsure

<222> LOCATION: (47)..(48)

<221> NAME/KEY: unsure

<222> LOCATION: (56)

<221> NAME/KEY: unsure

<222> LOCATION: (59)

<221> NAME/KEY: unsure

<222> LOCATION: (68)

<221> NAME/KEY: unsure

<222> LOCATION: (72)

<221> NAME/KEY: unsure

<222> LOCATION: (74)

<221> NAME/KEY: unsure

<222> LOCATION: (78)

<221> NAME/KEY: unsure

<222> LOCATION: (86)

<221> NAME/KEY: unsure

<222> LOCATION: (326)

<221> NAME/KEY: unsure

<222> LOCATION: (348)

<221> NAME/KEY: unsure

<222> LOCATION: (434)

<221> NAME/KEY: unsure

<222> LOCATION: (512)

<221> NAME/KEY: unsure

<222> LOCATION: (529)

<400> SEQUENCE: 145

tngatttcag gnacntcggn ttnaagtatc ngagtcgcnc agatatnnag atattnagng 60

atttcacntt gnanatcncc tccggnaaga ctgttgcact cgtgggagag agtggcagtg 120

gcaagtcaac ggtgatctct ttgctggaac ggttctacaa ccctgactct ggcaccatct 180

cactggatgg ggtcgagatc aagagcctga aagtcaattg gttgagggac cagatgggtc 240

tggtggggca agagccaatc ctcttcaacg acacgatccg tgccaacata gcctacggga 300

agcatgggga ggtaaccgag gagganttgt caaggtcgca agggcggnca acgcgcacga 360

gttcatatcg agcttcccca ggggtacgac accactgtcg gagagagaag ggtcagctat 420

ccggtggcca gaancagccg gtgccatagc gagagccata ctgaagacct agatttactc 480

ttgacgaggc aacgagcgcc tgacgccgaa tntagcgcat gtgcagatnc ctgacatgta 540

tgtcgcaga 549

<210> SEQ ID NO 146

<211> LENGTH: 127

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (82)

<221> NAME/KEY: UNSURE

<222> LOCATION: (84)

<221> NAME/KEY: UNSURE

<222> LOCATION: (90)

<221> NAME/KEY: UNSURE

<222> LOCATION: (98)

<221> NAME/KEY: UNSURE

<222> LOCATION: (117)

<221> NAME/KEY: UNSURE

<222> LOCATION: (119)

<400> SEQUENCE: 146

Ser Gly Lys Thr Val Ala Leu Val Gly Glu Ser Gly Ser Gly Lys Ser

1 5 10 15

Thr Val Ile Ser Leu Leu Glu Arg Phe Tyr Asn Pro Asp Ser Gly Thr

20 25 30

Ile Ser Leu Asp Gly Val Glu Ile Lys Ser Leu Lys Val Asn Trp Leu

35 40 45

Arg Asp Gln Met Gly Leu Val Gly Gln Glu Pro Ile Leu Phe Asn Asp

50 55 60

Thr Ile Arg Ala Asn Ile Ala Tyr Gly Lys His Gly Glu Val Thr Glu

65 70 75 80

Glu Xaa Leu Xaa Lys Val Ala Arg Ala Xaa Asn Ala His Glu Phe Ile

85 90 95

Ser Xaa Leu Pro Gln Gly Tyr Asp Thr Thr Val Gly Glu Arg Gln Leu

100 105 110

Ser Gly Gly Gln Xaa Gln Xaa Ala Ile Ala Arg Ala Ile Leu Lys

115 120 125

<210> SEQ ID NO 147

<211> LENGTH: 628

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (218)

<221> NAME/KEY: unsure

<222> LOCATION: (232)

<221> NAME/KEY: unsure

<222> LOCATION: (288)

<221> NAME/KEY: unsure

<222> LOCATION: (341)

<221> NAME/KEY: unsure

<222> LOCATION: (347)

<221> NAME/KEY: unsure

<222> LOCATION: (351)

<221> NAME/KEY: unsure

<222> LOCATION: (359)

<221> NAME/KEY: unsure

<222> LOCATION: (376)

<221> NAME/KEY: unsure

<222> LOCATION: (385)

<221> NAME/KEY: unsure

<222> LOCATION: (389)

<221> NAME/KEY: unsure

<222> LOCATION: (403)

<221> NAME/KEY: unsure

<222> LOCATION: (407)

<221> NAME/KEY: unsure

<222> LOCATION: (419)

<221> NAME/KEY: unsure

<222> LOCATION: (476)

<221> NAME/KEY: unsure

<222> LOCATION: (487)

<221> NAME/KEY: unsure

<222> LOCATION: (489)

<221> NAME/KEY: unsure

<222> LOCATION: (493)

<221> NAME/KEY: unsure

<222> LOCATION: (499)

<221> NAME/KEY: unsure

<222> LOCATION: (513)

<221> NAME/KEY: unsure

<222> LOCATION: (530)

<221> NAME/KEY: unsure

<222> LOCATION: (554)

<221> NAME/KEY: unsure

<222> LOCATION: (579)

<221> NAME/KEY: unsure

<222> LOCATION: (590)

<221> NAME/KEY: unsure

<222> LOCATION: (600)

›SEQUENCE LISTING · 39 of 53

<221> NAME/KEY: unsure

<222> LOCATION: (606)

<221> NAME/KEY: unsure

<222> LOCATION: (609)

<221> NAME/KEY: unsure

<222> LOCATION: (611)

<400> SEQUENCE: 147

cgcagtcagg gttacgccca ggtgaagttc ttgaaggggt tcagccaaga tgctaaggag 60

atgtacgagg atgcaagcca agtggcgact gatgctgtcg gcagcatcag aaccgtggcg 120

tcgttctgcg cagagaaaag agtggtcgca gcgtacagcg acaagtgtga ggctctgagg 180

aaacagggca tcccgaagcg gatcctccgg agggcttngg tacggttctc cntccttgat 240

gctgtccttc aacttatggc ctttgcttct acttccgtgc gcattttntt gcgccaaggg 300

aaaacaactt tccaaaagtt ttcaagtttc cttcccgtta nttcctngca nctattgant 360

ccccccaagc aattcnctgg ttccngatnc aacaaagcaa ggntccncat tccaacttna 420

cgttcttgac aggaatccaa attaccccac aacggaacgg atacccggag gatcantgga 480

aatcatnanc aantaactna attccacgct ccnactcaaa ttcacgactn acttccatcc 540

ttcggaaaaa atgnctttcg aaaattgacg aattcacana cccttctacn ttcaacaatn 600

cggaanccng nggtttaata accaatcc 628

<210> SEQ ID NO 148

<211> LENGTH: 66

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 148

Gly Tyr Ala Gln Val Lys Phe Leu Lys Gly Phe Ser Gln Asp Ala Lys

1 5 10 15

Glu Met Tyr Glu Asp Ala Ser Gln Val Ala Thr Asp Ala Val Gly Ser

20 25 30

Ile Arg Thr Val Ala Ser Phe Cys Ala Glu Lys Arg Val Val Ala Ala

35 40 45

Tyr Ser Asp Lys Cys Glu Ala Leu Arg Lys Gln Gly Ile Pro Lys Arg

50 55 60

Ile Leu

65

<210> SEQ ID NO 149

<211> LENGTH: 1016

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 149

gcacgagcca acttcgaaaa gattctcggt tttgggcact gacgtgcctt ctgtttgcag 60

tcattgccct gatttcaatc caactggaat actttctgtt tggggttgct ggtgggaaac 120

ttatacaacg tgtccgctct ttgtctttcc agagcatagt acatcaagaa gttgcttggt 180

ttgatgagcc ttcaaattct agtggggccc ttggcgcgag actctatatt gatgctttga 240

acatccgacg ccttgtcgga gataacttgg ccatactagt gcagtgtata gtaacaatag 300

cggctggttt ttccatagca tttgcttctg actggaaact cacgctgatc gtcatatgtg 360

tgattcccgt aatgggctca caaaattata ttcaagtgaa attcttgaaa gggttcagtg 420

aagatgctaa ggtgatgtat gaagatgcaa gtcaagtcgt aaccgaggca attggtagta 480

ttcaaacagt agcatctttc tgtgcagaga agagggtaat tacatcatac atccaaaaat 540

gccaagcttc aatgaaacat ggcattagaa gtggaatggt tggaggcctt ggtttcagtt 600

tatcaaactt aattatgtat ctcacatatg ctctttgttt ctatgttggt gcactgttcg 660

tacacgaagg aaaaacaact tttaaagacg tttttagggt ctattttgct ctgattttca 720

cagcttttgg agtttcccaa acaagtgcaa cggcaacaga ttcaacaaaa gcccaggaat 780

ccacaatttc aatactaact attatagaca ggagatctaa aatcaactcg actagtgatg 840

aaggcgtgat tatagaaaaa gttgatggca acatagattt caggcatgta agcttcaaat 900

acccatcccg cccagacgtc caagtgctca gcaactttac tttggccatt ccagcaagaa 960

agactgttgc acttgttgga gagagtggta gtggtaagtc aacaataatt tctttg 1016

<210> SEQ ID NO 150

<211> LENGTH: 338

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 150

Thr Ser Gln Leu Arg Lys Asp Ser Arg Phe Trp Ala Leu Thr Cys Leu

1 5 10 15

Leu Phe Ala Val Ile Ala Leu Ile Ser Ile Gln Leu Glu Tyr Phe Leu

20 25 30

Phe Gly Val Ala Gly Gly Lys Leu Ile Gln Arg Val Arg Ser Leu Ser

35 40 45

Phe Gln Ser Ile Val His Gln Glu Val Ala Trp Phe Asp Glu Pro Ser

50 55 60

Asn Ser Ser Gly Ala Leu Gly Ala Arg Leu Tyr Ile Asp Ala Leu Asn

65 70 75 80

Ile Arg Arg Leu Val Gly Asp Asn Leu Ala Ile Leu Val Gln Cys Ile

85 90 95

Val Thr Ile Ala Ala Gly Phe Ser Ile Ala Phe Ala Ser Asp Trp Lys

100 105 110

Leu Thr Leu Ile Val Ile Cys Val Ile Pro Val Met Gly Ser Gln Asn

115 120 125

Tyr Ile Gln Val Lys Phe Leu Lys Gly Phe Ser Glu Asp Ala Lys Val

130 135 140

Met Tyr Glu Asp Ala Ser Gln Val Val Thr Glu Ala Ile Gly Ser Ile

145 150 155 160

Gln Thr Val Ala Ser Phe Cys Ala Glu Lys Arg Val Ile Thr Ser Tyr

165 170 175

Ile Gln Lys Cys Gln Ala Ser Met Lys His Gly Ile Arg Ser Gly Met

180 185 190

Val Gly Gly Leu Gly Phe Ser Leu Ser Asn Leu Ile Met Tyr Leu Thr

195 200 205

Tyr Ala Leu Cys Phe Tyr Val Gly Ala Leu Phe Val His Glu Gly Lys

210 215 220

Thr Thr Phe Lys Asp Val Phe Arg Val Tyr Phe Ala Leu Ile Phe Thr

225 230 235 240

Ala Phe Gly Val Ser Gln Thr Ser Ala Thr Ala Thr Asp Ser Thr Lys

245 250 255

Ala Gln Glu Ser Thr Ile Ser Ile Leu Thr Ile Ile Asp Arg Arg Ser

260 265 270

Lys Ile Asn Ser Thr Ser Asp Glu Gly Val Ile Ile Glu Lys Val Asp

275 280 285

Gly Asn Ile Asp Phe Arg His Val Ser Phe Lys Tyr Pro Ser Arg Pro

290 295 300

Asp Val Gln Val Leu Ser Asn Phe Thr Leu Ala Ile Pro Ala Arg Lys

305 310 315 320

Thr Val Ala Leu Val Gly Glu Ser Gly Ser Gly Lys Ser Thr Ile Ile

325 330 335

Ser Leu

<210> SEQ ID NO 151

<211> LENGTH: 314

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 151

cttcatatgc agtttcccag accaatacaa gactgtcgtg ggagagcgag gcatcaggtt 60

atctgggcgg ggcagaagca gagggtcgcc attgcaagag ctttgcttat gaacccacga 120

gtgcttctct tagatgaagc taccagcgct ctggacgccg aaagcgagta ccttgttcag 180

gatgcaatgg actcgttgat gaaagggagg accgttcttg tgatagctca tcggctctcc 240

accgtgaaga gcgccgacac ggttgccgtc atctccgagg gccagattgt ggagagaggc 300

acacacgacg aact 314

<210> SEQ ID NO 152

<211> LENGTH: 103

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (22)

<400> SEQUENCE: 152

Phe Ile Cys Ser Phe Pro Asp Gln Tyr Lys Thr Val Val Gly Glu Arg

1 5 10 15

Gly Ile Arg Leu Ser Xaa Gly Gln Lys Gln Arg Val Ala Ile Ala Arg

20 25 30

Ala Leu Leu Met Asn Pro Arg Val Leu Leu Leu Asp Glu Ala Thr Ser

35 40 45

Ala Leu Asp Ala Glu Ser Glu Tyr Leu Val Gln Asp Ala Met Asp Ser

50 55 60

Leu Met Lys Gly Arg Thr Val Leu Val Ile Ala His Arg Leu Ser Thr

65 70 75 80

Val Lys Ser Ala Asp Thr Val Ala Val Ile Ser Glu Gly Gln Ile Val

85 90 95

Glu Arg Gly Thr His Asp Glu

100

<210> SEQ ID NO 153

<211> LENGTH: 942

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 153

gcacgagtgc acattcccta tggaaagact attgcactcg ttggagagag tggttgtggc 60

aagtcaactg taatctctct gctggagaga ttctacaatc ctgattcagg taccatttta 120

ttggatggag tggaaatcaa gagcttgaac atcaactggt tgaggaagca aactggacta 180

gtgagccaag agcctgtact cttcaatgac acaattcgtg ccaacatagc ctacggaaag 240

gatgaggaag tcagtgagga ggagctcatt gcagcggcga aggcatccaa tgcgcacgag 300

ttcatatcaa gccttcctca aggatacgaa acatctgttg gggagagagg gatacaacta 360

tccggtggcc agaagcagcg ggtggctatc gcaagggcca tactgaaaga cccgaagata 420

ctactacttg acgaggcgac cagcgccctg gatgctgaat ctgagcggat cgtgcagaat 480

gccttggatc atgtgatggt tggcaggacc acggttgccg tggcgcaccg cctctcgacg 540

atcaaaggcg ccgatgtcat tgcagtcctc aaggatggtg caattgtgga gaaaggggga 600

›SEQUENCE LISTING · 40 of 53

cacgagtccc tgatgaacat caaagatggg gtgtacgctt cactagttga acttcgctcg 660

gctcaccatg aaaatgccta gtagacgtac gcggtacacc atgcccaatt tgctgtatct 720

agcagtctaa gtctatttga tgtaagtttg ctcgcattta aaaggagatt ctgcttctgc 780

tttagatttt gattctggtt ctgaaacgta catttcctgc tatgtgtaga gcagtgtagt 840

tttgtgctgt aataactata gaaataattg aagatcgccc cttcagaaat tattgtatgg 900

agatgtctgt agctcttgtt tttcaaaaaa aaaaaaaaaa aa 942

<210> SEQ ID NO 154

<211> LENGTH: 226

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 154

Ala Arg Val His Ile Pro Tyr Gly Lys Thr Ile Ala Leu Val Gly Glu

1 5 10 15

Ser Gly Cys Gly Lys Ser Thr Val Ile Ser Leu Leu Glu Arg Phe Tyr

20 25 30

Asn Pro Asp Ser Gly Thr Ile Leu Leu Asp Gly Val Glu Ile Lys Ser

35 40 45

Leu Asn Ile Asn Trp Leu Arg Lys Gln Thr Gly Leu Val Ser Gln Glu

50 55 60

Pro Val Leu Phe Asn Asp Thr Ile Arg Ala Asn Ile Ala Tyr Gly Lys

65 70 75 80

Asp Glu Glu Val Ser Glu Glu Glu Leu Ile Ala Ala Ala Lys Ala Ser

85 90 95

Asn Ala His Glu Phe Ile Ser Ser Leu Pro Gln Gly Tyr Glu Thr Ser

100 105 110

Val Gly Glu Arg Gly Ile Gln Leu Ser Gly Gly Gln Lys Gln Arg Val

115 120 125

Ala Ile Ala Arg Ala Ile Leu Lys Asp Pro Lys Ile Leu Leu Leu Asp

130 135 140

Glu Ala Thr Ser Ala Leu Asp Ala Glu Ser Glu Arg Ile Val Gln Asn

145 150 155 160

Ala Leu Asp His Val Met Val Gly Arg Thr Thr Val Ala Val Ala His

165 170 175

Arg Leu Ser Thr Ile Lys Gly Ala Asp Val Ile Ala Val Leu Lys Asp

180 185 190

Gly Ala Ile Val Glu Lys Gly Gly His Glu Ser Leu Met Asn Ile Lys

195 200 205

Asp Gly Val Tyr Ala Ser Leu Val Glu Leu Arg Ser Ala His His Glu

210 215 220

Asn Ala

225

<210> SEQ ID NO 155

<211> LENGTH: 821

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 155

attctggtac aatctcactg gatggaacag aacttaaaaa actaaagctg agttggttaa 60

gagaccaaac gggcttagtg agccaagaac ctgtactttt caacaacaca attcgcacaa 120

acatagcata tggaaaacaa ggagaagtaa gagaggatga gatcgttgct gcggccaagg 180

cagcaaatgc tcatgagttc atatctagct tgcctcaggg atatagcact attgtcggcg 240

agaggggaac acagctctct ggaggacaaa aacaacgggt tgctattgca agggccatct 300

tgaaggaccc aaaaatactt ctactcgacg aggcaacaag tgctctggat gctgaaggag 360

agcacattgt tcaagatgca ctggaccaag tgatggttag caggaccacc attgttgtgg 420

cgcaccgact gtccacaatt aaaggggcag atatgatcgt agtcatgaaa gatggtgaag 480

ttgctgagaa ggggaagcat gaataccttg tgggaaaggg tggagtttat gcatcgctgg 540

tagaactaca ctccaagtca gcataaggat atatgggaac ttcgatggat tatttggtat 600

tacaaggaga gcatttgcag taggaggtag tcatgcaacg aaaacaaatc catgtgaaac 660

ttgtcaattc atacttctgg tttgttatta tagggcagtg agtcttatcc taaactcaat 720

gcagacaaag atagmattgg catcaccgta tttcatatgc tgcagtgcac ttgattatca 780

ataaattaat ggtaaaaagg agaaaaaaaa aaaaaaaaaa a 821

<210> SEQ ID NO 156

<211> LENGTH: 187

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 156

Ser Gly Thr Ile Ser Leu Asp Gly Thr Glu Leu Lys Lys Leu Lys Leu

1 5 10 15

Ser Trp Leu Arg Asp Gln Thr Gly Leu Val Ser Gln Glu Pro Val Leu

20 25 30

Phe Asn Asn Thr Ile Arg Thr Asn Ile Ala Tyr Gly Lys Gln Gly Glu

35 40 45

Val Arg Glu Asp Glu Ile Val Ala Ala Ala Lys Ala Ala Asn Ala His

50 55 60

Glu Phe Ile Ser Ser Leu Pro Gln Gly Tyr Ser Thr Ile Val Gly Glu

65 70 75 80

Arg Gly Thr Gln Leu Ser Gly Gly Gln Lys Gln Arg Val Ala Ile Ala

85 90 95

Arg Ala Ile Leu Lys Asp Pro Lys Ile Leu Leu Leu Asp Glu Ala Thr

100 105 110

Ser Ala Leu Asp Ala Glu Gly Glu His Ile Val Gln Asp Ala Leu Asp

115 120 125

Gln Val Met Val Ser Arg Thr Thr Ile Val Val Ala His Arg Leu Ser

130 135 140

Thr Ile Lys Gly Ala Asp Met Ile Val Val Met Lys Asp Gly Glu Val

145 150 155 160

Ala Glu Lys Gly Lys His Glu Tyr Leu Val Gly Lys Gly Gly Val Tyr

165 170 175

Ala Ser Leu Val Glu Leu His Ser Lys Ser Ala

180 185

<210> SEQ ID NO 157

<211> LENGTH: 688

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 157

gcacgagccg ctccgctccg ctcgtgctct gctcctgctc tctctctctc tcggcgtcga 60

ttgggcgacc gccgccgcct tccccaggtc ttcgctgggc ccgtcgccgg cgccgagcac 120

ccaccagggc aagttttact tgataatgtg gacatcaaga cgttgcaatt aaagcgactt 180

agagaccaga tcggattggt gaatcaggaa cctactcttt ttgcaactac aattcttgag 240

aatattcttt ttggcaaacc tgaggcgaca aaggcagagg ttgggtctac gactacttcc 300

gcagtagatt aaaggagccc tagctttcgg aggacaaccc agatgccttc aaagttcaaa 360

atgacggtga tgaaggtgga caagaaacaa aattttggat ggttcaaaat tttgctatag 420

gtcctagcaa cgcttatgtg ttgctatagg ggggttattt tgtagtgaac tcccaggcca 480

tatgctagct tactttgagg agtgcccact atttgtgcat gctacaggcg ctatcattac 540

atttccatga cctgtaaagt ggtgacatct ttaaatacag aatggccaca ttagcgaacc 600

agaggtttca cacgagtatt cccttgtgat gagaaagtcc catgtcaata atgtgacttc 660

ttttgttgat taaaaaaaaa aaaaaaaa 688

<210> SEQ ID NO 158

<211> LENGTH: 103

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 158

Ala Arg Ala Ala Pro Leu Arg Ser Cys Ser Ala Pro Ala Leu Ser Leu

1 5 10 15

Ser Arg Arg Arg Leu Gly Asp Arg Arg Arg Leu Pro Gln Val Phe Ala

20 25 30

Gly Pro Val Ala Gly Ala Glu His Pro Pro Gly Gln Val Leu Leu Asp

35 40 45

Asn Val Asp Ile Lys Thr Leu Gln Leu Lys Arg Leu Arg Asp Gln Ile

50 55 60

Gly Leu Val Asn Gln Glu Pro Thr Leu Phe Ala Thr Thr Ile Leu Glu

65 70 75 80

Asn Ile Leu Phe Gly Lys Pro Glu Ala Thr Lys Ala Glu Val Gly Ser

85 90 95

Thr Thr Thr Ser Ala Val Asp

100

<210> SEQ ID NO 159

<211> LENGTH: 1123

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 159

gcacgagata tttgcaattg tagatcgaaa atcaaggata gacccaagtg aggatgcagg 60

ggtaactgtt gaggcactac aaggaaacat cgtattccag catgttagct tcaagtatcc 120

taccaggcca gatgttcaga tattccggga cctttgcttg acaattcatg ctggaaagac 180

tgttgcactc gttggagaga gtggtagtgg caaatcaacg gcaatttcat tgctccagag 240

gttctatgac ccagatgtag gccatatact actagatggc gtggacatac agaagttcca 300

gcttaggtgg ttaaggcaac aaatgggcct ggttagtcaa gaaccagctt tgtttaatga 360

cacaataagg gcaaacattg cctatggaaa agatggacaa gcaacagaat ctgagatcat 420

atctgctgca gaattggcaa atgctcacaa gtttatcagt tcggcgctgc agggatatga 480

cacagtggtt ggagagcgtg gagcccagtt gtcaggaggg cagaagcagc gagtggcgat 540

cgcccgtgca attgtgaagg accctaggat cctgcttctg gacgaagcga ctagtgcgct 600

ggatgctgag tccgagcgga tcgtccagga cgcactggac agggtgatgg tgaaccggac 660

aacagtgatc gtcgctcacc gtctgtcaac aatacaaaat gcagatttga ttgcggtcgt 720

gaggaatggg gttatcatcg agaagggaaa gcacgatgcc ttgatcaaca tcaaggatgg 780

cgcctatgcg tccctcgtcg cccttcactc ggcagcctct tcatagcagg tgatggtatg 840

›SEQUENCE LISTING · 41 of 53

ggatcgtgca tacgtctgct aagacatata tgaacagagt gagctggcac aacctgtgta 900

gcaatgcagg tgttagtctc tgttcctcta tttgatggca tacataattg cttataccta 960

agcggagaaa tgttacaaat agcaaggacc agatgtaaat gcaaggagct ggtccttaag 1020

ataacttttt ataatctata aatcttaata tgtatatgta gcttcgaaca tagaaaccaa 1080

atagatgatg gttatgctat aaaaaaaaaa aaaaaaaaaa aaa 1123

<210> SEQ ID NO 160

<211> LENGTH: 274

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 160

His Glu Ile Phe Ala Ile Val Asp Arg Lys Ser Arg Ile Asp Pro Ser

1 5 10 15

Glu Asp Ala Gly Val Thr Val Glu Ala Leu Gln Gly Asn Ile Val Phe

20 25 30

Gln His Val Ser Phe Lys Tyr Pro Thr Arg Pro Asp Val Gln Ile Phe

35 40 45

Arg Asp Leu Cys Leu Thr Ile His Ala Gly Lys Thr Val Ala Leu Val

50 55 60

Gly Glu Ser Gly Ser Gly Lys Ser Thr Ala Ile Ser Leu Leu Gln Arg

65 70 75 80

Phe Tyr Asp Pro Asp Val Gly His Ile Leu Leu Asp Gly Val Asp Ile

85 90 95

Gln Lys Phe Gln Leu Arg Trp Leu Arg Gln Gln Met Gly Leu Val Ser

100 105 110

Gln Glu Pro Ala Leu Phe Asn Asp Thr Ile Arg Ala Asn Ile Ala Tyr

115 120 125

Gly Lys Asp Gly Gln Ala Thr Glu Ser Glu Ile Ile Ser Ala Ala Glu

130 135 140

Leu Ala Asn Ala His Lys Phe Ile Ser Ser Ala Leu Gln Gly Tyr Asp

145 150 155 160

Thr Val Val Gly Glu Arg Gly Ala Gln Leu Ser Gly Gly Gln Lys Gln

165 170 175

Arg Val Ala Ile Ala Arg Ala Ile Val Lys Asp Pro Arg Ile Leu Leu

180 185 190

Leu Asp Glu Ala Thr Ser Ala Leu Asp Ala Glu Ser Glu Arg Ile Val

195 200 205

Gln Asp Ala Leu Asp Arg Val Met Val Asn Arg Thr Thr Val Ile Val

210 215 220

Ala His Arg Leu Ser Thr Ile Gln Asn Ala Asp Leu Ile Ala Val Val

225 230 235 240

Arg Asn Gly Val Ile Ile Glu Lys Gly Lys His Asp Ala Leu Ile Asn

245 250 255

Ile Lys Asp Gly Ala Tyr Ala Ser Leu Val Ala Leu His Ser Ala Ala

260 265 270

Ser Ser

<210> SEQ ID NO 161

<211> LENGTH: 1185

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 161

gcacgaggtt agcttcaagt accctatgag acctgatatc accatatttc agaacttaaa 60

cctcatagtc ccagcaggta agagtctggc agtagtgggg caaagtggtt cagggaaaag 120

cacagtgatt tctttggtaa tgagatttta cgaccccgat ttggggtcag tcctaataga 180

tgaatgtgat atcaaaagcc taaaccttag atccttaagg ctgagaatag gattggttca 240

gcaagaacct gctttgttct caaccacagt ttatgaaaac atcaagtatg gaaaagagga 300

ggcatcagaa atagaggtaa tgaaggcagc caaagcagca aatgctcatg aattcattag 360

cagaatgcca gaagggtaca aaactgaggt tggtgagaga ggggcgcagt tgtcaggagg 420

acaaaaacaa agagtggcaa ttgctagagc tattctgaaa gatccatcca ttcttttgtt 480

ggatgaagca acaagtgcac tagacacagt atcagagagg ctggtccaag aggctcttga 540

taagcttatg gaaggtagaa caactatttt agtagctcac aggctatcaa ctgttcgcga 600

tgccgacagc attgcagtgc ttcaaaatgg cagggttgct gaaatgggaa gccatgagag 660

gctgatggcc aaacctgcaa gcatctacaa gcaattggtt agtctacagc atgaaacacg 720

tgaccaacaa gaccattgat gaattattca tttgcagctt cacaattgta gaaattcaaa 780

gcgtacttgc aatttacaac ttgaattgag gcaaggccta gtttattctc aaaagcatgc 840

ctgaaatatg ttttttttca taccacaatc atttttctgt ttatatacac gagtactatg 900

ataaaattca aagcctatgg ttggctgttt gttgtagaat gctacaactt ttgtaatttc 960

tttttgaatt ttacaagatc tttggacaat caaaatttgt tcttttctca ttttcactcc 1020

atcctccttc aaatctcatg aaactttttt tttagttaca agatctttga tccttccttt 1080

tcactaaatg gtagcttttc atggtttcac cttcttcatt tatgtttgtc atcatcaata 1140

aaagtactac gtgccaaccg ttttgacaaa aaaaaaaaaa aaaaa 1185

<210> SEQ ID NO 162

<211> LENGTH: 245

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 162

His Glu Val Ser Phe Lys Tyr Pro Met Arg Pro Asp Ile Thr Ile Phe

1 5 10 15

Gln Asn Leu Asn Leu Ile Val Pro Ala Gly Lys Ser Leu Ala Val Val

20 25 30

Gly Gln Ser Gly Ser Gly Lys Ser Thr Val Ile Ser Leu Val Met Arg

35 40 45

Phe Tyr Asp Pro Asp Leu Gly Ser Val Leu Ile Asp Glu Cys Asp Ile

50 55 60

Lys Ser Leu Asn Leu Arg Ser Leu Arg Leu Arg Ile Gly Leu Val Gln

65 70 75 80

Gln Glu Pro Ala Leu Phe Ser Thr Thr Val Tyr Glu Asn Ile Lys Tyr

85 90 95

Gly Lys Glu Glu Ala Ser Glu Ile Glu Val Met Lys Ala Ala Lys Ala

100 105 110

Ala Asn Ala His Glu Phe Ile Ser Arg Met Pro Glu Gly Tyr Lys Thr

115 120 125

Glu Val Gly Glu Arg Gly Ala Gln Leu Ser Gly Gly Gln Lys Gln Arg

130 135 140

Val Ala Ile Ala Arg Ala Ile Leu Lys Asp Pro Ser Ile Leu Leu Leu

145 150 155 160

Asp Glu Ala Thr Ser Ala Leu Asp Thr Val Ser Glu Arg Leu Val Gln

165 170 175

Glu Ala Leu Asp Lys Leu Met Glu Gly Arg Thr Thr Ile Leu Val Ala

180 185 190

His Arg Leu Ser Thr Val Arg Asp Ala Asp Ser Ile Ala Val Leu Gln

195 200 205

Asn Gly Arg Val Ala Glu Met Gly Ser His Glu Arg Leu Met Ala Lys

210 215 220

Pro Ala Ser Ile Tyr Lys Gln Leu Val Ser Leu Gln His Glu Thr Arg

225 230 235 240

Asp Gln Gln Asp His

245

<210> SEQ ID NO 163

<211> LENGTH: 918

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 163

agaacttaaa cctcagagtc ccagcaggca agagtctggc agtagtgggg caaagtggtt 60

caggaaaaag cacagtgatt tctttggtaa tgagatttta tgaccccgat tcggggttag 120

tcctggtaga tgaatgtgat atcaaaaacc taaacctgag atccttaagg ctgagaatag 180

gattggttca gcaagaacct gctttgttct ctaccacagt ttatgaaaac atcaagtatg 240

gaaaagagga ggcatcagaa atagaggtaa tgaaggcagc caaagcagca aatgctcatg 300

aattcatcag cagaatgcca gaagggtaca aaaccgaggt tggtgagaga ggggtgcagt 360

tgtcaggagg acaaaaacaa agagtggcaa ttgctagagc tatcctgaaa gatccatcca 420

ttcttttgtt ggacgaagca acaagtgcac ttgacacagt atcagagagg ctagtccaag 480

aggctcttga taaacttatg gaaggtagaa caactatttt ggtagctcac aggttatcaa 540

ctgttcgcga tgccaacagc attgcagtgc ttcaaaatgg cagggttgct gaaatgggga 600

gccatgagag gctgatggcc aaatccggaa gcatctacaa gcaattggtt agtctacagc 660

atgaaacacg cgaccaagaa gatcattgat gaattattca ttcgcaattt cccaagtaat 720

tgtagaaatt cgaagtgtac ttgcaattcg caacttgaat tgaggcaagg cctagttcat 780

tctcaagaac atgcctgaaa gatgtttttt tttccttctt cataggacag ttgttttctg 840

tttatataca cgagtcctat gataaaattc aaagcctatg attgtttgtt tgttgtaaaa 900

aaaaaaaaaa aaaaaaaa 918

<210> SEQ ID NO 164

<211> LENGTH: 228

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 164

Asn Leu Asn Leu Arg Val Pro Ala Gly Lys Ser Leu Ala Val Val Gly

1 5 10 15

Gln Ser Gly Ser Gly Lys Ser Thr Val Ile Ser Leu Val Met Arg Phe

20 25 30

Tyr Asp Pro Asp Ser Gly Leu Val Leu Val Asp Glu Cys Asp Ile Lys

35 40 45

Asn Leu Asn Leu Arg Ser Leu Arg Leu Arg Ile Gly Leu Val Gln Gln

50 55 60

Glu Pro Ala Leu Phe Ser Thr Thr Val Tyr Glu Asn Ile Lys Tyr Gly

65 70 75 80

Lys Glu Glu Ala Ser Glu Ile Glu Val Met Lys Ala Ala Lys Ala Ala

›SEQUENCE LISTING · 42 of 53

85 90 95

Asn Ala His Glu Phe Ile Ser Arg Met Pro Glu Gly Tyr Lys Thr Glu

100 105 110

Val Gly Glu Arg Gly Val Gln Leu Ser Gly Gly Gln Lys Gln Arg Val

115 120 125

Ala Ile Ala Arg Ala Ile Leu Lys Asp Pro Ser Ile Leu Leu Leu Asp

130 135 140

Glu Ala Thr Ser Ala Leu Asp Thr Val Ser Glu Arg Leu Val Gln Glu

145 150 155 160

Ala Leu Asp Lys Leu Met Glu Gly Arg Thr Thr Ile Leu Val Ala His

165 170 175

Arg Leu Ser Thr Val Arg Asp Ala Asn Ser Ile Ala Val Leu Gln Asn

180 185 190

Gly Arg Val Ala Glu Met Gly Ser His Glu Arg Leu Met Ala Lys Ser

195 200 205

Gly Ser Ile Tyr Lys Gln Leu Val Ser Leu Gln His Glu Thr Arg Asp

210 215 220

Gln Glu Asp His

225

<210> SEQ ID NO 165

<211> LENGTH: 578

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (390)

<221> NAME/KEY: unsure

<222> LOCATION: (500)

<221> NAME/KEY: unsure

<222> LOCATION: (508)

<221> NAME/KEY: unsure

<222> LOCATION: (516)

<221> NAME/KEY: unsure

<222> LOCATION: (529)

<221> NAME/KEY: unsure

<222> LOCATION: (543)

<221> NAME/KEY: unsure

<222> LOCATION: (557)

<221> NAME/KEY: unsure

<222> LOCATION: (575)

<400> SEQUENCE: 165

cttacacggc acgagcttac agccaatgct catgaattca ttgtctctct tccacagggc 60

tatgacacac ttgttggtga acgtggcagc ctcctaagtg ggggacagag acagagaatt 120

gctattgctc gtgctcttct gaaaaactct cctattctaa tacttgatga ggctaccagt 180

gcacttgata cgaccagtga acggcttgta caggaagctc tcaatcactt gatgaaggga 240

aggacttctc tggtgatcgc gcacagattg agcaccgtgc aaaatgcgca tcaaatcgct 300

gtctgctcag atggcaagat agcagaactt gggactcatg tgaactggtt ctagcggtgg 360

ccgtatgcgt cacttgttgg tacacagagn tcgcatttga gtaagcaact tcagaaaatt 420

ctgttccgaa agacgttccg acgagttctc gacatattta catcatctga catatatacg 480

ggatggttac gaacacatgn catgaatnac gaaacncatg ttcaagttnc actcaaaacg 540

agnctcatag tgtgccnaaa aaagtgtttt gaccnatt 578

<210> SEQ ID NO 166

<211> LENGTH: 106

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 166

Ala Asn Ala His Glu Phe Ile Val Ser Leu Pro Gln Gly Tyr Asp Thr

1 5 10 15

Leu Val Gly Glu Arg Gly Ser Leu Leu Ser Gly Gly Gln Arg Gln Arg

20 25 30

Ile Ala Ile Ala Arg Ala Leu Leu Lys Asn Ser Pro Ile Leu Ile Leu

35 40 45

Asp Glu Ala Thr Ser Ala Leu Asp Thr Thr Ser Glu Arg Leu Val Gln

50 55 60

Glu Ala Leu Asn His Leu Met Lys Gly Arg Thr Ser Leu Val Ile Ala

65 70 75 80

His Arg Leu Ser Thr Val Gln Asn Ala His Gln Ile Ala Val Cys Ser

85 90 95

Asp Gly Lys Ile Ala Glu Leu Gly Thr His

100 105

<210> SEQ ID NO 167

<211> LENGTH: 1428

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 167

gcacgagatt ttgtgatgca gaatgccgtg gttttgttat atttggctgg tgggtctttc 60

attgcttgtt tcctagaggg ttattgttgg acaagaacag gagaaaggca agctgcaaga 120

atgagagtta ggtaccttaa agcagttctc aggcaagaag tagcatactt tgatttgcat 180

gtcacaagca catcggaggt catcaccagc gtctctaatg atagcctcgt aattcaagat 240

tgtcttagtg aaaaggtccc aaactttttg atgaatgcgt ccatgtttgt tgggagctac 300

atagtggctt ttgcattatt gtggagattg gccattgtgg ggttcccttt tgtggcccta 360

cttgtgatcc ccggtttcat gtatgggagg acattaatgg ggttggctag caagataaga 420

gaagagtaca ataaagcagg cacaatagca gaacaagcaa tatcctccat cagaaccgtt 480

tattcttttg tgggggaaag caagactatt gatgctttct ctgaagccct acaagggtct 540

gttgagttgg gactgagaca aggcttagca aaaggtttag ctattggaag caatggtgtt 600

gtctttgcta tatgggcatt catgtcctat tatggtagca gattggtcat gtaccatgga 660

gctaaaggtg ggactgtatt tgcagttgga gcagccatag ctcttggagg attggcacta 720

ggtgctggtt tgtcgaacgt gaagtacttc tcagaagcaa gtaccgcagg agaacgcata 780

atggaagtga taaaaagggt tccaaagatt gattctgata gcatggctga ggagattctg 840

gagaacgttt caggggaagt tgaattcaac catgtggact ttgtgtaccc atcaaggcca 900

gacagtgtta ttctgaatga tttctgccta aagattccag cagggaaaac agtggctttg 960

gttggaggga gtggctctgg aaaatccact gtgatatcac ttttgcagag gttttatgac 1020

ccaattgagg gagagatatt tcttgatggt gtggccattc acaagttgca actcaagtgg 1080

ttgaggtctc aaatgggttt ggtcagccaa gagcctgcac tgtttgcaac tagcattaaa 1140

gagaatatac tttttggaag agaagatgcc actcaagaag aggttgtgga ggcagcaaaa 1200

gcttccaatg ctcataattt catttcacag ttgccacaag gatatgatac tcaggtgagt 1260

gctacttcat tagtcatttg tcaaaccaac ccaatatgtt agtagttagg ttaggatgtt 1320

tgtttgtgga gtgtgtgtca cggcatgcaa gttttaccct tggtggttgt taggatgaaa 1380

aaagaagtaa aaaaattcaa attaaactat aaaaaaaaaa aaaaaaaa 1428

<210> SEQ ID NO 168

<211> LENGTH: 433

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 168

Ala Arg Asp Phe Val Met Gln Asn Ala Val Val Leu Leu Tyr Leu Ala

1 5 10 15

Gly Gly Ser Phe Ile Ala Cys Phe Leu Glu Gly Tyr Cys Trp Thr Arg

20 25 30

Thr Gly Glu Arg Gln Ala Ala Arg Met Arg Val Arg Tyr Leu Lys Ala

35 40 45

Val Leu Arg Gln Glu Val Ala Tyr Phe Asp Leu His Val Thr Ser Thr

50 55 60

Ser Glu Val Ile Thr Ser Val Ser Asn Asp Ser Leu Val Ile Gln Asp

65 70 75 80

Cys Leu Ser Glu Lys Val Pro Asn Phe Leu Met Asn Ala Ser Met Phe

85 90 95

Val Gly Ser Tyr Ile Val Ala Phe Ala Leu Leu Trp Arg Leu Ala Ile

100 105 110

Val Gly Phe Pro Phe Val Ala Leu Leu Val Ile Pro Gly Phe Met Tyr

115 120 125

Gly Arg Thr Leu Met Gly Leu Ala Ser Lys Ile Arg Glu Glu Tyr Asn

130 135 140

Lys Ala Gly Thr Ile Ala Glu Gln Ala Ile Ser Ser Ile Arg Thr Val

145 150 155 160

Tyr Ser Phe Val Gly Glu Ser Lys Thr Ile Asp Ala Phe Ser Glu Ala

165 170 175

Leu Gln Gly Ser Val Glu Leu Gly Leu Arg Gln Gly Leu Ala Lys Gly

180 185 190

Leu Ala Ile Gly Ser Asn Gly Val Val Phe Ala Ile Trp Ala Phe Met

195 200 205

Ser Tyr Tyr Gly Ser Arg Leu Val Met Tyr His Gly Ala Lys Gly Gly

210 215 220

Thr Val Phe Ala Val Gly Ala Ala Ile Ala Leu Gly Gly Leu Ala Leu

225 230 235 240

Gly Ala Gly Leu Ser Asn Val Lys Tyr Phe Ser Glu Ala Ser Thr Ala

245 250 255

Gly Glu Arg Ile Met Glu Val Ile Lys Arg Val Pro Lys Ile Asp Ser

260 265 270

Asp Ser Met Ala Glu Glu Ile Leu Glu Asn Val Ser Gly Glu Val Glu

275 280 285

Phe Asn His Val Asp Phe Val Tyr Pro Ser Arg Pro Asp Ser Val Ile

290 295 300

Leu Asn Asp Phe Cys Leu Lys Ile Pro Ala Gly Lys Thr Val Ala Leu

305 310 315 320

Val Gly Gly Ser Gly Ser Gly Lys Ser Thr Val Ile Ser Leu Leu Gln

325 330 335

Arg Phe Tyr Asp Pro Ile Glu Gly Glu Ile Phe Leu Asp Gly Val Ala

340 345 350

Ile His Lys Leu Gln Leu Lys Trp Leu Arg Ser Gln Met Gly Leu Val

355 360 365

Ser Gln Glu Pro Ala Leu Phe Ala Thr Ser Ile Lys Glu Asn Ile Leu

370 375 380

Phe Gly Arg Glu Asp Ala Thr Gln Glu Glu Val Val Glu Ala Ala Lys

385 390 395 400

›SEQUENCE LISTING · 43 of 53

Ala Ser Asn Ala His Asn Phe Ile Ser Gln Leu Pro Gln Gly Tyr Asp

405 410 415

Thr Gln Val Ser Ala Thr Ser Leu Val Ile Cys Gln Thr Asn Pro Ile

420 425 430

Cys

<210> SEQ ID NO 169

<211> LENGTH: 1995

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 169

agctcgggct ccctccccaa tccccatccc caaatcgctt tcgctccgcc ccggtcgccg 60

gaccatggcc gcctccgcct caaccacgct cttctcaccg tgcgccctca cctccaagct 120

cggggcagga cacgcgtcct atggctccgt acgccgcgct aacgtgcgcc ggcgcggccg 180

cctctcggtg gtggccgtac aaacgggccc gcagaagccg tcgccgttgt cgtcccaggc 240

cgccgcggcg gaggatgagg cggacgcgct gcagaagctt ctgaagaggg agtacaaata 300

cgggtttgtg tccgacttcg agtccttctc catccccaag gggctgtcgg aggccaccgt 360

ccgccgcatc tcggagctca aggcggagcc ggcgtggatg ctggacttcc gcctcgccgc 420

ctaccgccgc ttcctcacca tggtcgagcc cacctggagc gacaacgagt acgcgcccgt 480

ggacctgcag tctatctgct actactccgc gcccaaaacc aagcccaagc tcaatagcct 540

cgacgaggtc gacccggagc tgctcaatac cttcgaccgc ctcggcatcc cgctcagcga 600

gcagaagcgc ctcgccaacg tcgctgtcga cgccgtcatc gactccactt ccatcgccac 660

cacccaccgc gaggcgctca tggccaaggg cgtcatcttc tgctccatct ccgaggccat 720

ccgtgagtac ccggaccttg tcaggcgcta cctcggcagc atcgtaccgc cgggcgacaa 780

ctactatgcc gccctcaatt cagcggtgtt cagcgacgga tccttctgct acgtgcccaa 840

ggacacggtc tgccccatgg aaatttcgac ttacttcagg atcaacgaca aggagacagg 900

ccagtttgag aggactctga ttgtagccga tgagagaagc actgttagct atttggaagg 960

ctgcactgca ccagcatatg actcaaatca gctccatgct gcggttgtgg agcttgtgtg 1020

tgagaatggg gcagagatta agtactccac ggtgcagaat tggtactccg gtgatgagga 1080

ggggagagga ggcatttata actttgtgac aaagagggga aggtgcaaag ggcggggttc 1140

gaagatctca tggacacagg ttgagacagg atctgctatt acatggaagt acccaagcgt 1200

tgagctcgtc ggggatgaca gtgttggaga gttctactcg gttgcgctta caaaggattg 1260

ccagcaagca gacacaggga cgaaaatgat ccacaagggg aaaaattcac gcagccggat 1320

tatatccaag ggcatctctg cagggaagtc aaggaattgc taccgtggct tggtcctgat 1380

gaactctagt gcagagaatg cttataattc ttcacagtgt gactcattgt tgatcgggga 1440

taatgcggct gcgaacacct atcccaccat tcaggtgggg tgcactagtg gccgggttga 1500

gcatgaggca agcacttcca aaattggaga ggatcagcta ttttatttcc agcaaagagg 1560

ggtagatcat gaaaaggcag ttgccgccat gattggtgga ttctgcagat ctgttttcga 1620

gcaccttccc tatgaattcg cacaggaggt ggacgcactc atgaacctga aactcgaggg 1680

atcagttggc taatttgttg gctcagcgat ttcgtgtaat actccatgac agccagttag 1740

ctagaaaatc catgtgtaca gcagtacgag aagaataaat tacaggataa gtatacattt 1800

cctggctggt tgtaatactg aatgttgatg atgtctatgg caattggcaa cgaactatga 1860

agatgtagca gttgagcctg tttgtcttct gttgcccagt ttttgctatt gctgtaagcg 1920

agctcacatg gctcacctat acaagaacgg aaatcaacgt gttggcgtta cagaaaaaaa 1980

aaaaaaaaaa aaaaa 1995

<210> SEQ ID NO 170

<211> LENGTH: 563

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 170

Ala Arg Ala Pro Ser Pro Ile Pro Ile Pro Lys Ser Leu Ser Leu Arg

1 5 10 15

Pro Gly Arg Arg Thr Met Ala Ala Ser Ala Ser Thr Thr Leu Phe Ser

20 25 30

Pro Cys Ala Leu Thr Ser Lys Leu Gly Ala Gly His Ala Ser Tyr Gly

35 40 45

Ser Val Arg Arg Ala Asn Val Arg Arg Arg Gly Arg Leu Ser Val Val

50 55 60

Ala Val Gln Thr Gly Pro Gln Lys Pro Ser Pro Leu Ser Ser Gln Ala

65 70 75 80

Ala Ala Ala Glu Asp Glu Ala Asp Ala Leu Gln Lys Leu Leu Lys Arg

85 90 95

Glu Tyr Lys Tyr Gly Phe Val Ser Asp Phe Glu Ser Phe Ser Ile Pro

100 105 110

Lys Gly Leu Ser Glu Ala Thr Val Arg Arg Ile Ser Glu Leu Lys Ala

115 120 125

Glu Pro Ala Trp Met Leu Asp Phe Arg Leu Ala Ala Tyr Arg Arg Phe

130 135 140

Leu Thr Met Val Glu Pro Thr Trp Ser Asp Asn Glu Tyr Ala Pro Val

145 150 155 160

Asp Leu Gln Ser Ile Cys Tyr Tyr Ser Ala Pro Lys Thr Lys Pro Lys

165 170 175

Leu Asn Ser Leu Asp Glu Val Asp Pro Glu Leu Leu Asn Thr Phe Asp

180 185 190

Arg Leu Gly Ile Pro Leu Ser Glu Gln Lys Arg Leu Ala Asn Val Ala

195 200 205

Val Asp Ala Val Ile Asp Ser Thr Ser Ile Ala Thr Thr His Arg Glu

210 215 220

Ala Leu Met Ala Lys Gly Val Ile Phe Cys Ser Ile Ser Glu Ala Ile

225 230 235 240

Arg Glu Tyr Pro Asp Leu Val Arg Arg Tyr Leu Gly Ser Ile Val Pro

245 250 255

Pro Gly Asp Asn Tyr Tyr Ala Ala Leu Asn Ser Ala Val Phe Ser Asp

260 265 270

Gly Ser Phe Cys Tyr Val Pro Lys Asp Thr Val Cys Pro Met Glu Ile

275 280 285

Ser Thr Tyr Phe Arg Ile Asn Asp Lys Glu Thr Gly Gln Phe Glu Arg

290 295 300

Thr Leu Ile Val Ala Asp Glu Arg Ser Thr Val Ser Tyr Leu Glu Gly

305 310 315 320

Cys Thr Ala Pro Ala Tyr Asp Ser Asn Gln Leu His Ala Ala Val Val

325 330 335

Glu Leu Val Cys Glu Asn Gly Ala Glu Ile Lys Tyr Ser Thr Val Gln

340 345 350

Asn Trp Tyr Ser Gly Asp Glu Glu Gly Arg Gly Gly Ile Tyr Asn Phe

355 360 365

Val Thr Lys Arg Gly Arg Cys Lys Gly Arg Gly Ser Lys Ile Ser Trp

370 375 380

Thr Gln Val Glu Thr Gly Ser Ala Ile Thr Trp Lys Tyr Pro Ser Val

385 390 395 400

Glu Leu Val Gly Asp Asp Ser Val Gly Glu Phe Tyr Ser Val Ala Leu

405 410 415

Thr Lys Asp Cys Gln Gln Ala Asp Thr Gly Thr Lys Met Ile His Lys

420 425 430

Gly Lys Asn Ser Arg Ser Arg Ile Ile Ser Lys Gly Ile Ser Ala Gly

435 440 445

Lys Ser Arg Asn Cys Tyr Arg Gly Leu Val Leu Met Asn Ser Ser Ala

450 455 460

Glu Asn Ala Tyr Asn Ser Ser Gln Cys Asp Ser Leu Leu Ile Gly Asp

465 470 475 480

Asn Ala Ala Ala Asn Thr Tyr Pro Thr Ile Gln Val Gly Cys Thr Ser

485 490 495

Gly Arg Val Glu His Glu Ala Ser Thr Ser Lys Ile Gly Glu Asp Gln

500 505 510

Leu Phe Tyr Phe Gln Gln Arg Gly Val Asp His Glu Lys Ala Val Ala

515 520 525

Ala Met Ile Gly Gly Phe Cys Arg Ser Val Phe Glu His Leu Pro Tyr

530 535 540

Glu Phe Ala Gln Glu Val Asp Ala Leu Met Asn Leu Lys Leu Glu Gly

545 550 555 560

Ser Val Gly

<210> SEQ ID NO 171

<211> LENGTH: 2043

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 171

gcacgagtac acacaacaac ccacccctcc tgcgctctcc atcccatccg catccctaat 60

ttcgcctcgg cctcgccacg ccgccgcacc atggccgcag ccgccgcgtc gtccacgccc 120

ctcttctcgc cctgctgcgc cgccgcgacc gccaagctcg gggcggcgtg cccgtcgtcg 180

tacggatccc ggcggaggcc gtgcacgcgc cgtggacgcc tctcggtggt ggccgtgcag 240

acgggcccgc agaagccatc gccgtcgtcg tcgtcccagg cggggacgga atcggagacg 300

ctgcagaacc tgctgaagag ggagtacaag tatgggttcg tgtctgattt cgagtccttc 360

tccatcccca aggggctctc cgaggccacg gtccgccgca tctcggagct caaggcggag 420

ccggcgtgga tgctcgactt ccgcctcgcc gcctaccgcc ggttcctgac catggtgcag 480

cccacctgga gcgacaacgt gtacgagccg gtggatctcc agtccatctg ctactactcc 540

gcgcccaaga ccaagcccaa gctcaacagc ctcgacgagg tcgacccgga gcttctcaac 600

›SEQUENCE LISTING · 44 of 53

accttcgatc gcctcgggat cccgctcagc gagcagaagc gcctcgccaa cgtcgccgtc 660

gatgccgtca tcgactccac ctccatcgcc accacccacc gggaggagct catgaagaag 720

ggcgtcatct tttgctccat ttccgaggcc atccgcgagt acccggacct tgtcaagcgc 780

taccttggca gcgtcgtgcc gccggcagat aactactatg ctgccctcaa ttcggccgtg 840

ttcagcgatg ggtcgttctg ctatgtgccc aaggatacgg tatgcccaat ggagatatcg 900

acatacttta ggatcaacga caaggagacc gggcaatttg agaggactct aattgtggct 960

gatgagagga gcacggttag ctatctggaa gggtgtactg ctccagctta tgactccaac 1020

cagctccatg cggcggttgt ggagcttgtg tgtgaggagc aggcagagat caagtactct 1080

acggtgcaga attggtactc tggtgatgag gaggggaagg ggggcattta caactttgtt 1140

actaagagag gacggtgcaa agggcggggc tctaagatct catggacaca ggttgagaca 1200

ggttcagcaa ttacatggaa gtacccaagt gtggagcttc ttggggatga tactgttgga 1260

gagttctact cagttgcact gacgaaggat taccagcagg cagacacagg gacaaaaatg 1320

atccacaagg gaaagaattc gcggagtcgg attatatcca aaggtatctc agctgggaag 1380

tcacggaatt gctaccgtgg gttggtccag ataaactcag gcgcagaaaa tgcttataat 1440

tcttcacagt gtgattcttt gctgattggg gataacgctg ctgccaacac ctatccgact 1500

attcaggtgg gttgcatttc tagtcgtgtt gagcatgagg caagcacttc aaaaataggg 1560

gaggaccagc tattttattt ccaacaaagg gggatagacc atgaaaaggc tgttgcagcg 1620

atgattggtg gattctgcag ggctgttttc gagaatcttc cttacgagtt tgcacatgag 1680

atggatgcac ttatgaacct gaagcttgag ggatcagttg gttaattaga tggagcaaaa 1740

tgtctttgca gtacaacaca acaattattt agatgtcctt tgtacagaag tatgagaaga 1800

ataaattgca tgagcaatgg acgttttcct agccttgtct accactaggg atgtccttgg 1860

caacaaagaa aattctgtta cttatctact gttttatccc tttttgtttc tgtttcttct 1920

gttgtaaaca aacccttatt agaatgcgta gccacactag aaatgtctcc aaaaaagcta 1980

cacaagaaat ggaatctgct tttttaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2040

aaa 2043

<210> SEQ ID NO 172

<211> LENGTH: 574

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 172

Ala Arg Val His Thr Thr Thr His Pro Ser Cys Ala Leu His Pro Ile

1 5 10 15

Arg Ile Pro Asn Phe Ala Ser Ala Ser Pro Arg Arg Arg Thr Met Ala

20 25 30

Ala Ala Ala Ala Ser Ser Thr Pro Leu Phe Ser Pro Cys Cys Ala Ala

35 40 45

Ala Thr Ala Lys Leu Gly Ala Ala Cys Pro Ser Ser Tyr Gly Ser Arg

50 55 60

Arg Arg Pro Cys Thr Arg Arg Gly Arg Leu Ser Val Val Ala Val Gln

65 70 75 80

Thr Gly Pro Gln Lys Pro Ser Pro Ser Ser Ser Ser Gln Ala Gly Thr

85 90 95

Glu Ser Glu Thr Leu Gln Asn Leu Leu Lys Arg Glu Tyr Lys Tyr Gly

100 105 110

Phe Val Ser Asp Phe Glu Ser Phe Ser Ile Pro Lys Gly Leu Ser Glu

115 120 125

Ala Thr Val Arg Arg Ile Ser Glu Leu Lys Ala Glu Pro Ala Trp Met

130 135 140

Leu Asp Phe Arg Leu Ala Ala Tyr Arg Arg Phe Leu Thr Met Val Gln

145 150 155 160

Pro Thr Trp Ser Asp Asn Val Tyr Glu Pro Val Asp Leu Gln Ser Ile

165 170 175

Cys Tyr Tyr Ser Ala Pro Lys Thr Lys Pro Lys Leu Asn Ser Leu Asp

180 185 190

Glu Val Asp Pro Glu Leu Leu Asn Thr Phe Asp Arg Leu Gly Ile Pro

195 200 205

Leu Ser Glu Gln Lys Arg Leu Ala Asn Val Ala Val Asp Ala Val Ile

210 215 220

Asp Ser Thr Ser Ile Ala Thr Thr His Arg Glu Glu Leu Met Lys Lys

225 230 235 240

Gly Val Ile Phe Cys Ser Ile Ser Glu Ala Ile Arg Glu Tyr Pro Asp

245 250 255

Leu Val Lys Arg Tyr Leu Gly Ser Val Val Pro Pro Ala Asp Asn Tyr

260 265 270

Tyr Ala Ala Leu Asn Ser Ala Val Phe Ser Asp Gly Ser Phe Cys Tyr

275 280 285

Val Pro Lys Asp Thr Val Cys Pro Met Glu Ile Ser Thr Tyr Phe Arg

290 295 300

Ile Asn Asp Lys Glu Thr Gly Gln Phe Glu Arg Thr Leu Ile Val Ala

305 310 315 320

Asp Glu Arg Ser Thr Val Ser Tyr Leu Glu Gly Cys Thr Ala Pro Ala

325 330 335

Tyr Asp Ser Asn Gln Leu His Ala Ala Val Val Glu Leu Val Cys Glu

340 345 350

Glu Gln Ala Glu Ile Lys Tyr Ser Thr Val Gln Asn Trp Tyr Ser Gly

355 360 365

Asp Glu Glu Gly Lys Gly Gly Ile Tyr Asn Phe Val Thr Lys Arg Gly

370 375 380

Arg Cys Lys Gly Arg Gly Ser Lys Ile Ser Trp Thr Gln Val Glu Thr

385 390 395 400

Gly Ser Ala Ile Thr Trp Lys Tyr Pro Ser Val Glu Leu Leu Gly Asp

405 410 415

Asp Thr Val Gly Glu Phe Tyr Ser Val Ala Leu Thr Lys Asp Tyr Gln

420 425 430

Gln Ala Asp Thr Gly Thr Lys Met Ile His Lys Gly Lys Asn Ser Arg

435 440 445

Ser Arg Ile Ile Ser Lys Gly Ile Ser Ala Gly Lys Ser Arg Asn Cys

450 455 460

Tyr Arg Gly Leu Val Gln Ile Asn Ser Gly Ala Glu Asn Ala Tyr Asn

465 470 475 480

Ser Ser Gln Cys Asp Ser Leu Leu Ile Gly Asp Asn Ala Ala Ala Asn

485 490 495

Thr Tyr Pro Thr Ile Gln Val Gly Cys Ile Ser Ser Arg Val Glu His

500 505 510

Glu Ala Ser Thr Ser Lys Ile Gly Glu Asp Gln Leu Phe Tyr Phe Gln

515 520 525

Gln Arg Gly Ile Asp His Glu Lys Ala Val Ala Ala Met Ile Gly Gly

530 535 540

Phe Cys Arg Ala Val Phe Glu Asn Leu Pro Tyr Glu Phe Ala His Glu

545 550 555 560

Met Asp Ala Leu Met Asn Leu Lys Leu Glu Gly Ser Val Gly

565 570

<210> SEQ ID NO 173

<211> LENGTH: 1917

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 173

gcacgaggtt tttttttttt tttttttttt tttttttttt tttttttttg aggtggtaca 60

aaacctcatc catggcttct cttctccgct tcaacggcgt ctccctcttt cccttacctt 120

cccaacccac acgtgacccc atcctcccca aaccgtcacg cctcgtaacg gtgcgcgcgg 180

aggtgaaaga gacctccgca cccgccgccg ccacatcctc cgacgaaaag atccgcgaaa 240

tcctccgcaa ccgtgactac gacaagaaat tcgggttcag cgtggagatc gagtccttga 300

cgatcccaaa agggctctca aaagaaacca ttggtctgat ctcgtccctg aaggaggagc 360

cccactggat gctcgaattc cgcctgaagg ccttggataa attcctctcc atgaaggaac 420

ccacgtggtc tgacaacact taccctccca tcgacctcca gaatatctgc tactactccg 480

cccccaagaa taaaccctct cttcaatccc tcgacgacgc tgaccccgag cttctccgct 540

acttcgagaa gctcggcgtc cccctcaacg agcagaagcg cctcgccaac gtcgccgtcg 600

acgccgtcct cgacagcgtc tcaatcgcca caacgcaccg caaaacccta gaaaaggccg 660

gcgttatatt ctgctccatc tccgaggcca ttaaggaata tcctgatttg gtgaaaaagt 720

atttggggaa agttgtcccg tcagacgaca actactacgc cgcgttgaac gctgcggtct 780

tcagcgacgg gtcgttttgc tacattccta aagacaccaa gtgtcctatg cagatttcga 840

cttacttcag aatcaacgcg ttggagacag gacagtttga gaggactttg attgtcgccg 900

acgatcggag ctctgtggag tatttggaag gctgcaccgc gccttcctac gatcggaacc 960

agctccatgc cgcggtggtg gagttgtatt gcggcgaggg ggcggagatt aagtactcca 1020

cggtgcagaa ttggtacgct ggggacgaag aagggaaggg tggggtttac aatttcgtga 1080

ccaagagagg gttgtgtggt ggtgcaggtt cgaagatatc gtggacgcag gtggagacgg 1140

gttcggcgat cacgtggaag tacccgagtg ttgtgctgga aggagatggt agtgttgggg 1200

agttctattc tgtggctttg acgaataact atcagcaggc ggataccggg accaagatga 1260

tacacaaagg gaagaacacg aggagtagga ttatatccaa aggaatatcg gttgggcatt 1320

›SEQUENCE LISTING · 45 of 53

ccaggaactg ttatagaggg cttgttcagg ttctgtccaa ggctgacaat gcccgaaact 1380

cgtcacagtg tgattctatg cttattggtg acaatgctgc tgcaaatacc tatccttata 1440

tccaggtgaa aaatccaacc gcaagaattg aacatgaagc tagtacatcc aaaattggag 1500

aagatcaatt gttttatttt caacaaaggg gaatagacta tgaaaaggca atggctgcta 1560

tgatctctgg attttgccgt gatgtgttta atgagcttcc tgatgaattt ggttccgaag 1620

tgaaccaact catgagcttg aagcttgagg gatcagtagg ttaaacagca tatcactatt 1680

tggaatttac tcaggccatt tgttcttgaa attgtccaag tccttagagg tagtgtattc 1740

gaaaggatgt gtacatgttg tatatagata ctgtcttcaa ctgtaccgca aaagtggcct 1800

gaaacattac cagttttgaa tcttaataaa caatcaatgt catcctataa ttttgtttgt 1860

cgatttactc ttgatttata ttcatgtatc cctataaaaa aaaaaaaaaa aaaaaaa 1917

<210> SEQ ID NO 174

<211> LENGTH: 530

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 174

Met Ala Ser Leu Leu Arg Phe Asn Gly Val Ser Leu Phe Pro Leu Pro

1 5 10 15

Ser Gln Pro Thr Arg Asp Pro Ile Leu Pro Lys Pro Ser Arg Leu Val

20 25 30

Thr Val Arg Ala Glu Val Lys Glu Thr Ser Ala Pro Ala Ala Ala Thr

35 40 45

Ser Ser Asp Glu Lys Ile Arg Glu Ile Leu Arg Asn Arg Asp Tyr Asp

50 55 60

Lys Lys Phe Gly Phe Ser Val Glu Ile Glu Ser Leu Thr Ile Pro Lys

65 70 75 80

Gly Leu Ser Lys Glu Thr Ile Gly Leu Ile Ser Ser Leu Lys Glu Glu

85 90 95

Pro His Trp Met Leu Glu Phe Arg Leu Lys Ala Leu Asp Lys Phe Leu

100 105 110

Ser Met Lys Glu Pro Thr Trp Ser Asp Asn Thr Tyr Pro Pro Ile Asp

115 120 125

Leu Gln Asn Ile Cys Tyr Tyr Ser Ala Pro Lys Asn Lys Pro Ser Leu

130 135 140

Gln Ser Leu Asp Asp Ala Asp Pro Glu Leu Leu Arg Tyr Phe Glu Lys

145 150 155 160

Leu Gly Val Pro Leu Asn Glu Gln Lys Arg Leu Ala Asn Val Ala Val

165 170 175

Asp Ala Val Leu Asp Ser Val Ser Ile Ala Thr Thr His Arg Lys Thr

180 185 190

Leu Glu Lys Ala Gly Val Ile Phe Cys Ser Ile Ser Glu Ala Ile Lys

195 200 205

Glu Tyr Pro Asp Leu Val Lys Lys Tyr Leu Gly Lys Val Val Pro Ser

210 215 220

Asp Asp Asn Tyr Tyr Ala Ala Leu Asn Ala Ala Val Phe Ser Asp Gly

225 230 235 240

Ser Phe Cys Tyr Ile Pro Lys Asp Thr Lys Cys Pro Met Gln Ile Ser

245 250 255

Thr Tyr Phe Arg Ile Asn Ala Leu Glu Thr Gly Gln Phe Glu Arg Thr

260 265 270

Leu Ile Val Ala Asp Asp Arg Ser Ser Val Glu Tyr Leu Glu Gly Cys

275 280 285

Thr Ala Pro Ser Tyr Asp Arg Asn Gln Leu His Ala Ala Val Val Glu

290 295 300

Leu Tyr Cys Gly Glu Gly Ala Glu Ile Lys Tyr Ser Thr Val Gln Asn

305 310 315 320

Trp Tyr Ala Gly Asp Glu Glu Gly Lys Gly Gly Val Tyr Asn Phe Val

325 330 335

Thr Lys Arg Gly Leu Cys Gly Gly Ala Gly Ser Lys Ile Ser Trp Thr

340 345 350

Gln Val Glu Thr Gly Ser Ala Ile Thr Trp Lys Tyr Pro Ser Val Val

355 360 365

Leu Glu Gly Asp Gly Ser Val Gly Glu Phe Tyr Ser Val Ala Leu Thr

370 375 380

Asn Asn Tyr Gln Gln Ala Asp Thr Gly Thr Lys Met Ile His Lys Gly

385 390 395 400

Lys Asn Thr Arg Ser Arg Ile Ile Ser Lys Gly Ile Ser Val Gly His

405 410 415

Ser Arg Asn Cys Tyr Arg Gly Leu Val Gln Val Leu Ser Lys Ala Asp

420 425 430

Asn Ala Arg Asn Ser Ser Gln Cys Asp Ser Met Leu Ile Gly Asp Asn

435 440 445

Ala Ala Ala Asn Thr Tyr Pro Tyr Ile Gln Val Lys Asn Pro Thr Ala

450 455 460

Arg Ile Glu His Glu Ala Ser Thr Ser Lys Ile Gly Glu Asp Gln Leu

465 470 475 480

Phe Tyr Phe Gln Gln Arg Gly Ile Asp Tyr Glu Lys Ala Met Ala Ala

485 490 495

Met Ile Ser Gly Phe Cys Arg Asp Val Phe Asn Glu Leu Pro Asp Glu

500 505 510

Phe Gly Ser Glu Val Asn Gln Leu Met Ser Leu Lys Leu Glu Gly Ser

515 520 525

Val Gly

530

<210> SEQ ID NO 175

<211> LENGTH: 1919

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 175

ttcgcctccg cctccgcctc gccggccgcg accgaccatg gcggcctccg cctccgcctc 60

cacgtccctc ttcgggccct gcgccctcac cgccaggctc ggggcggggc ggccgtccgg 120

ggggccacgc cggcgcgtcg acgcgcgcgg ccgcggccgc ctctcggtgg tggccgtgca 180

gacgggcccg cagaagccgt cgccgtcgcc ctcgccctcg tccccggcgg cggacgaggc 240

cgaggcgctg cagaacctgc tcaagcggga gtacaagtac ggcttcgtct ccgacttcga 300

gtccttctcc atccccaagg gcctctccga ggccaccgtc cgccgcatct cggagctcaa 360

ggcggagccg gcctggatgc tcgacttccg cctcgccgcc taccgccgct tcctcaccat 420

ggcggagccc acctggagcg acaacgtcta ctcgcccgtc gacctccagt ccctctgctt 480

ctactccgcg cccaagacca agcccaagct caacagcctc gacgaggtcg accccgagct 540

gctcaagacc ttcgaccgcc tcgggatccc tctcggcgag cagaagcgcc tctccaacgt 600

cgccgtcgac gccgtcatcg actccacctc catcgccacc acccaccggg aggcgctcat 660

ggccaagggc gtcatatttt gctccatctc cgaggccatc cgtgagtacc cggacctcat 720

caagcgctac atcgggagca tcgtgccgcc cggtgacaat tactatgccg cgctcaattc 780

agccgtgttc agtgacggat ccttctgcta cgtgcccaag gatacggtct gccccatgga 840

gatatcaacc tacttcagaa tcaacgacaa ggagaccggg cagtttgaga ggactctgat 900

tgtggctgat gagaggagca cggttagcta tttagaaggt tgtacagctc cagcatatga 960

ctccaaccag ctccacgccg cggtggtgga gcttgtgtgc gaagaagggg ccgagattaa 1020

gtactccacc gtgcagaatt ggtatgctgg cgatgaggag ggcaaagggg gcatttacaa 1080

ttttgtggcc aagagggggc ggtgcaaggg gcgtggctcg aaaatctcat ggacacaggt 1140

tgagacaggg tcagcaatca cctggaagta cccaagtgtg gagcttgttg gggacgacac 1200

tgttggagag ttctactccg tagcactgcc caaggattac cagcaggccg atacagggac 1260

aaagatgatc cataagggga agaattcacg cagccggatt atatccaaag gtatctcggc 1320

aggaaagtca aggaattgct accgtgggct ggtccagatg aattcaggtg cagagaatgc 1380

ttataattct tcgcagtgtg attcattgct gattggggac aacgctgccg ccaacaccta 1440

tccgaccatt caggtgggtt gcactagtgg ccgtgttgag catgaggcaa gcacatccaa 1500

aattggcgag gatcagctat tctactttca gcaaagaggg attgatcatg aaaaggccgt 1560

tgcagccatg ataggtgggt tctgtagagc cgtctttgaa caccttcctt acgagtttgc 1620

ccaggaggtg gatgcactaa tgaacctgaa gctggaggga tcagttggct aagttattgg 1680

ttcaacaagt ctgcgaaata tcacccgata attatctgaa ataccatacg atggttatct 1740

aaggaagtcc atgtgaccat gtacagcagc agtatgagac tgagaagaat aagttacatg 1800

ataaaaggac gtttcatggc ttgtataatc gtccacgaca gcgaagagaa ccgcggcgct 1860

tctcttttgt tgtccatttt aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaa 1919

<210> SEQ ID NO 176

<211> LENGTH: 544

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 176

Met Ala Ala Ser Ala Ser Ala Ser Thr Ser Leu Phe Gly Pro Cys Ala

1 5 10 15

Leu Thr Ala Arg Leu Gly Ala Gly Arg Pro Ser Gly Gly Pro Arg Arg

20 25 30

Arg Val Asp Ala Arg Gly Arg Gly Arg Leu Ser Val Val Ala Val Gln

35 40 45

Thr Gly Pro Gln Lys Pro Ser Pro Ser Pro Ser Pro Ser Ser Pro Ala

50 55 60

Ala Asp Glu Ala Glu Ala Leu Gln Asn Leu Leu Lys Arg Glu Tyr Lys

›SEQUENCE LISTING · 46 of 53

65 70 75 80

Tyr Gly Phe Val Ser Asp Phe Glu Ser Phe Ser Ile Pro Lys Gly Leu

85 90 95

Ser Glu Ala Thr Val Arg Arg Ile Ser Glu Leu Lys Ala Glu Pro Ala

100 105 110

Trp Met Leu Asp Phe Arg Leu Ala Ala Tyr Arg Arg Phe Leu Thr Met

115 120 125

Ala Glu Pro Thr Trp Ser Asp Asn Val Tyr Ser Pro Val Asp Leu Gln

130 135 140

Ser Leu Cys Phe Tyr Ser Ala Pro Lys Thr Lys Pro Lys Leu Asn Ser

145 150 155 160

Leu Asp Glu Val Asp Pro Glu Leu Leu Lys Thr Phe Asp Arg Leu Gly

165 170 175

Ile Pro Leu Gly Glu Gln Lys Arg Leu Ser Asn Val Ala Val Asp Ala

180 185 190

Val Ile Asp Ser Thr Ser Ile Ala Thr Thr His Arg Glu Ala Leu Met

195 200 205

Ala Lys Gly Val Ile Phe Cys Ser Ile Ser Glu Ala Ile Arg Glu Tyr

210 215 220

Pro Asp Leu Ile Lys Arg Tyr Ile Gly Ser Ile Val Pro Pro Gly Asp

225 230 235 240

Asn Tyr Tyr Ala Ala Leu Asn Ser Ala Val Phe Ser Asp Gly Ser Phe

245 250 255

Cys Tyr Val Pro Lys Asp Thr Val Cys Pro Met Glu Ile Ser Thr Tyr

260 265 270

Phe Arg Ile Asn Asp Lys Glu Thr Gly Gln Phe Glu Arg Thr Leu Ile

275 280 285

Val Ala Asp Glu Arg Ser Thr Val Ser Tyr Leu Glu Gly Cys Thr Ala

290 295 300

Pro Ala Tyr Asp Ser Asn Gln Leu His Ala Ala Val Val Glu Leu Val

305 310 315 320

Cys Glu Glu Gly Ala Glu Ile Lys Tyr Ser Thr Val Gln Asn Trp Tyr

325 330 335

Ala Gly Asp Glu Glu Gly Lys Gly Gly Ile Tyr Asn Phe Val Ala Lys

340 345 350

Arg Gly Arg Cys Lys Gly Arg Gly Ser Lys Ile Ser Trp Thr Gln Val

355 360 365

Glu Thr Gly Ser Ala Ile Thr Trp Lys Tyr Pro Ser Val Glu Leu Val

370 375 380

Gly Asp Asp Thr Val Gly Glu Phe Tyr Ser Val Ala Leu Pro Lys Asp

385 390 395 400

Tyr Gln Gln Ala Asp Thr Gly Thr Lys Met Ile His Lys Gly Lys Asn

405 410 415

Ser Arg Ser Arg Ile Ile Ser Lys Gly Ile Ser Ala Gly Lys Ser Arg

420 425 430

Asn Cys Tyr Arg Gly Leu Val Gln Met Asn Ser Gly Ala Glu Asn Ala

435 440 445

Tyr Asn Ser Ser Gln Cys Asp Ser Leu Leu Ile Gly Asp Asn Ala Ala

450 455 460

Ala Asn Thr Tyr Pro Thr Ile Gln Val Gly Cys Thr Ser Gly Arg Val

465 470 475 480

Glu His Glu Ala Ser Thr Ser Lys Ile Gly Glu Asp Gln Leu Phe Tyr

485 490 495

Phe Gln Gln Arg Gly Ile Asp His Glu Lys Ala Val Ala Ala Met Ile

500 505 510

Gly Gly Phe Cys Arg Ala Val Phe Glu His Leu Pro Tyr Glu Phe Ala

515 520 525

Gln Glu Val Asp Ala Leu Met Asn Leu Lys Leu Glu Gly Ser Val Gly

530 535 540

<210> SEQ ID NO 177

<211> LENGTH: 1314

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 177

ggagaccgag ctttgacttc aactcaagga gagttggcac atgctctgag gttcttggca 60

tctgtggtgt cacaaagctt catagcgttt ggtgatattc ttgagttaca caagaagttc 120

cttgaactct ctggtggtat taacaggata tttgagcttg aggagcttct acaggcatca 180

caaagcaatc ctgtcatgcc ttctgatgcc acaaatgccg gttccgaaga aataatttcc 240

ttccgtggcg tgaacattgt gacaccatcg caaaagctat tggctagcca actgtcttgt 300

gatgtatctc aagggaaaag ccttcttgtg actggtccaa atggtagcgg aaagagttcc 360

atctttaggg tgcttcgagg cttgtggccc attgcttctg gtagtcttag caagccatct 420

gaaggaattt ttaatgttcc tcaacgtcca tatacttgtc ttggaacctt gagggatcag 480

atcatatacc ctctctctca cgaggaggca aagttgaaga tgctctcagg cgaaacaagt 540

gacaagtcta cagcttccga gctgttggat gatcacctga ggacgattct agagaatgtt 600

cgcttgctgt atcttctgga gagagaaggt tgggatgcta ccactaactg ggaagatact 660

ctatccttgg gagagcagca gaggctgggg atggctcgtc tgttctttca ctgtcccaag 720

tatggcatcc tcgacgagtg caccaatgcc acaagcgtgg acgttgagga gcatctgtac 780

aggctagcaa ccaacatggg cataacagtc atcacatcct cacaaaggcc tgctctgatt 840

cccttccatt cgttggaact gaaactcatt gacggcgaag gaaagtggga gctatgttcc 900

atccaccaat aaccgattga catcaaccag tttatttatg ttcatcggag tagggtattt 960

acaagttgca acgcacagag aaaaaaaaaa ctcgtccact cgggtgtaga gtgtgtgacg 1020

gagtcttttt ttttttttgg tttccctttt ctttttctct cccctttgat ggaaatcttg 1080

cgatacggaa agggagggag gaaggaaata tacggcagga aaggaaagta ttaggagagg 1140

ttatagatgc aggcttcgtc atttgggata gcatagtaca tgttcctggc atgatatgcc 1200

agcctgattg atttttatac gagatacaga ataagttaaa ataaaaatgg atatggcaca 1260

aagtgtgccc ttgtaaatta aatgggaaac taatctatag gaaattcatc caaa 1314

<210> SEQ ID NO 178

<211> LENGTH: 303

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 178

Gly Asp Arg Ala Leu Thr Ser Thr Gln Gly Glu Leu Ala His Ala Leu

1 5 10 15

Arg Phe Leu Ala Ser Val Val Ser Gln Ser Phe Ile Ala Phe Gly Asp

20 25 30

Ile Leu Glu Leu His Lys Lys Phe Leu Glu Leu Ser Gly Gly Ile Asn

35 40 45

Arg Ile Phe Glu Leu Glu Glu Leu Leu Gln Ala Ser Gln Ser Asn Pro

50 55 60

Val Met Pro Ser Asp Ala Thr Asn Ala Gly Ser Glu Glu Ile Ile Ser

65 70 75 80

Phe Arg Gly Val Asn Ile Val Thr Pro Ser Gln Lys Leu Leu Ala Ser

85 90 95

Gln Leu Ser Cys Asp Val Ser Gln Gly Lys Ser Leu Leu Val Thr Gly

100 105 110

Pro Asn Gly Ser Gly Lys Ser Ser Ile Phe Arg Val Leu Arg Gly Leu

115 120 125

Trp Pro Ile Ala Ser Gly Ser Leu Ser Lys Pro Ser Glu Gly Ile Phe

130 135 140

Asn Val Pro Gln Arg Pro Tyr Thr Cys Leu Gly Thr Leu Arg Asp Gln

145 150 155 160

Ile Ile Tyr Pro Leu Ser His Glu Glu Ala Lys Leu Lys Met Leu Ser

165 170 175

Gly Glu Thr Ser Asp Lys Ser Thr Ala Ser Glu Leu Leu Asp Asp His

180 185 190

Leu Arg Thr Ile Leu Glu Asn Val Arg Leu Leu Tyr Leu Leu Glu Arg

195 200 205

Glu Gly Trp Asp Ala Thr Thr Asn Trp Glu Asp Thr Leu Ser Leu Gly

210 215 220

Glu Gln Gln Arg Leu Gly Met Ala Arg Leu Phe Phe His Cys Pro Lys

225 230 235 240

Tyr Gly Ile Leu Asp Glu Cys Thr Asn Ala Thr Ser Val Asp Val Glu

245 250 255

Glu His Leu Tyr Arg Leu Ala Thr Asn Met Gly Ile Thr Val Ile Thr

260 265 270

Ser Ser Gln Arg Pro Ala Leu Ile Pro Phe His Ser Leu Glu Leu Lys

275 280 285

Leu Ile Asp Gly Glu Gly Lys Trp Glu Leu Cys Ser Ile His Gln

290 295 300

<210> SEQ ID NO 179

<211> LENGTH: 1126

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 179

gcacgagctt acacaaacat caaattcagg tcctgatgag ccatccaaca tacagtcaaa 60

cggtgaagaa ctgctacaaa gttcaaagca gagaagggat aatggtatat tctttgttcc 120

acagagacca tatatggttt tgggaaccct tcgtcagcaa ttgctctatc ctacatggac 180

tgaagatgtt tgtcattcat caaataatga tcctcaaagt acagaccctc ttacctttga 240

agtctccaca tcagatggag ttggttccaa gtctgagatg cccacaactg atgaactgat 300

cagagtactt gaggctgtta agttaggcta tatattgcct cgtttcaatg gtttggattc 360

tatgcatgat tgggccagcg ttctctctct gggggagcaa cagcgtcttg catttgctcg 420

gttgttactt gcaaaaccaa ctttagtcct ccttgatgag tccacaagtg cactagatga 480

tatgaatgag cgtcatctat acagtcagat tgaagctgca gggattactt acataagcat 540

tggtcaccgg aaaacactgc acaagttcca caacaaggtc ttgtacattt caaattccga 600

›SEQUENCE LISTING · 47 of 53

ttcaacggat agcaacccac gtaattggta cctaaagccc acagaacaga tgtcgattga 660

agagtcatcc tcatttgctt cataagaatt tcatgatttg agcaacattt gcccctgagc 720

agttttgtcg caagatttca cccatacgtg aagtgtatgg tgcccagcca agtacccaaa 780

atatgtcatg ctgattgttc taaagatgtg atgcttgagt agttgagttg tatgaccaaa 840

attgctagta tgctacaaac caactcgagg cgtggcacag attttagcac ctaaaggttg 900

aaagggaagc tcatctgttc ttggatcgtt ataccacgaa gttcagaatg aacatcatat 960

gtagatatgc agtcctctga tctttttgag tttcttttag aaatgacgac ggaatatctg 1020

aaactgtctc tttgttatta cgtgtgtggt gtgtccttgt aactatactg ggaattacag 1080

ctcttatata catgcagtgt gtgcaaaaaa aaaaaaaaaa aaaaaa 1126

<210> SEQ ID NO 180

<211> LENGTH: 227

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 180

His Glu Leu Thr Gln Thr Ser Asn Ser Gly Pro Asp Glu Pro Ser Asn

1 5 10 15

Ile Gln Ser Asn Gly Glu Glu Leu Leu Gln Ser Ser Lys Gln Arg Arg

20 25 30

Asp Asn Gly Ile Phe Phe Val Pro Gln Arg Pro Tyr Met Val Leu Gly

35 40 45

Thr Leu Arg Gln Gln Leu Leu Tyr Pro Thr Trp Thr Glu Asp Val Cys

50 55 60

His Ser Ser Asn Asn Asp Pro Gln Ser Thr Asp Pro Leu Thr Phe Glu

65 70 75 80

Val Ser Thr Ser Asp Gly Val Gly Ser Lys Ser Glu Met Pro Thr Thr

85 90 95

Asp Glu Leu Ile Arg Val Leu Glu Ala Val Lys Leu Gly Tyr Ile Leu

100 105 110

Pro Arg Phe Asn Gly Leu Asp Ser Met His Asp Trp Ala Ser Val Leu

115 120 125

Ser Leu Gly Glu Gln Gln Arg Leu Ala Phe Ala Arg Leu Leu Leu Ala

130 135 140

Lys Pro Thr Leu Val Leu Leu Asp Glu Ser Thr Ser Ala Leu Asp Asp

145 150 155 160

Met Asn Glu Arg His Leu Tyr Ser Gln Ile Glu Ala Ala Gly Ile Thr

165 170 175

Tyr Ile Ser Ile Gly His Arg Lys Thr Leu His Lys Phe His Asn Lys

180 185 190

Val Leu Tyr Ile Ser Asn Ser Asp Ser Thr Asp Ser Asn Pro Arg Asn

195 200 205

Trp Tyr Leu Lys Pro Thr Glu Gln Met Ser Ile Glu Glu Ser Ser Ser

210 215 220

Phe Ala Ser

225

<210> SEQ ID NO 181

<211> LENGTH: 1118

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 181

tgacatgtga cattgagctt ggtaaaagcc tgcttgttac tggtccaaat ggaagtggaa 60

aaagttccat tttccgagtt cttagaggac tttggccaat tgcaagtgga agactctcta 120

gaccatctga ggatgtggat caagaggctg gatcaggttg tggcatcttc tatgttcctc 180

agcggcctta cacatgcttg ggaacattgc gggatcaaat tatataccca ttgtctcgtg 240

aagaggcaca gtttcgagca ctgaagatgc atggaaaagg tgaaaaacat cctgacccta 300

gaaaaatgtt ggacacacac ttgcaagtta tcctagagaa tgttcggttg aattatcttc 360

tagaaagaga caacaatggc tgggatgcaa atctaaactg ggaagacatt ctctctcttg 420

gagaacagca gagattaggc atggcacgct tgttcttcca taagcctaaa tttggtatcc 480

tggacgagtg taccaatgcc actagcgttg atgttgaaga acacctatat gggctagcga 540

acaaaatggg gatcactgtt gttacttcct cacaacgtcc tgctttaata ccatttcatt 600

ccatggaatt gcgtctgatt gatggtgagg gtaattggga acttcgttcg atcaagcaat 660

gactttcgca cctgcagatt ctcattgtta ctatttgaga tgttgctgag tcttcgaaca 720

tacagaatga ttgcaagcag atattaggct tgccatgatg gtcattcctt tatatttttt 780

tttcttcttt tttctttttt cattttgggt ttaccatatt ctccaagctt gctccgtcga 840

cttgtgggga caaacaaaca aaaacagcat cgatagccaa aatgattgat agtaagttgg 900

agtgcacaac cttttcatga tctctgcagt atataatcaa ttatgtacag ggttttcgct 960

tctattatta gttacttaga tatttattca cattttgtaa caattggatg taattattaa 1020

tgttatttta tcaataaaac ggcacgaaac aggagtagct tttagagatt aaaaaaaaaa 1080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 1118

<210> SEQ ID NO 182

<211> LENGTH: 219

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 182

Thr Cys Asp Ile Glu Leu Gly Lys Ser Leu Leu Val Thr Gly Pro Asn

1 5 10 15

Gly Ser Gly Lys Ser Ser Ile Phe Arg Val Leu Arg Gly Leu Trp Pro

20 25 30

Ile Ala Ser Gly Arg Leu Ser Arg Pro Ser Glu Asp Val Asp Gln Glu

35 40 45

Ala Gly Ser Gly Cys Gly Ile Phe Tyr Val Pro Gln Arg Pro Tyr Thr

50 55 60

Cys Leu Gly Thr Leu Arg Asp Gln Ile Ile Tyr Pro Leu Ser Arg Glu

65 70 75 80

Glu Ala Gln Phe Arg Ala Leu Lys Met His Gly Lys Gly Glu Lys His

85 90 95

Pro Asp Pro Arg Lys Met Leu Asp Thr His Leu Gln Val Ile Leu Glu

100 105 110

Asn Val Arg Leu Asn Tyr Leu Leu Glu Arg Asp Asn Asn Gly Trp Asp

115 120 125

Ala Asn Leu Asn Trp Glu Asp Ile Leu Ser Leu Gly Glu Gln Gln Arg

130 135 140

Leu Gly Met Ala Arg Leu Phe Phe His Lys Pro Lys Phe Gly Ile Leu

145 150 155 160

Asp Glu Cys Thr Asn Ala Thr Ser Val Asp Val Glu Glu His Leu Tyr

165 170 175

Gly Leu Ala Asn Lys Met Gly Ile Thr Val Val Thr Ser Ser Gln Arg

180 185 190

Pro Ala Leu Ile Pro Phe His Ser Met Glu Leu Arg Leu Ile Asp Gly

195 200 205

Glu Gly Asn Trp Glu Leu Arg Ser Ile Lys Gln

210 215

<210> SEQ ID NO 183

<211> LENGTH: 788

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 183

gcacgaggcc aagcctgaga tgccctcaac tgatgaactg atcagagtac ttgaggttgt 60

taggctgggc tacatattac cacgtttcaa tggcatggat tctgtccatg attgggctag 120

tgttctttcc ctaggggagc aacagcgtct tgcatttgct cggttgttac tcgcaaaacc 180

aactctggtc ctactggacg agtccacaag tgcactagat gatacaaatg aggttcatct 240

atacagtcag attgaagctg cagggattac atatataagt gtcggtcacc ggaagacact 300

tcacaggttc cacaacaagg ttttgtacat ttcaaagtcc gattcggcaa ctggcagcct 360

tcgtaattgg gagttaaagc ccacagacca gaaatcaatt gaagaatcat ccccatttgc 420

ttcataagaa cttcatgcga accagtttag gccccttcct caatattctt atcccacatt 480

taacccatta tggtaaatat tgttgcccta ccaaggatcc catctccacg agttcaacgt 540

tacaacttac aaactggagc tggactgttg gcgaggttga tcttacagct ggactgacaa 600

aattgtggca gtgctactaa ccaacctgat agcaggatac gttctaggac ttggaataat 660

ctcacctttt tgtttatgtt tatgcttcat tgtttagaat gaaatataac atatgtttat 720

gtacggttga tattgcgcgc tgttaccaac attttaattt ttattatctg aaaaaaaaaa 780

aaaaaaaa 788

<210> SEQ ID NO 184

<211> LENGTH: 141

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 184

His Glu Ala Lys Pro Glu Met Pro Ser Thr Asp Glu Leu Ile Arg Val

1 5 10 15

Leu Glu Val Val Arg Leu Gly Tyr Ile Leu Pro Arg Phe Asn Gly Met

20 25 30

Asp Ser Val His Asp Trp Ala Ser Val Leu Ser Leu Gly Glu Gln Gln

35 40 45

Arg Leu Ala Phe Ala Arg Leu Leu Leu Ala Lys Pro Thr Leu Val Leu

50 55 60

Leu Asp Glu Ser Thr Ser Ala Leu Asp Asp Thr Asn Glu Val His Leu

65 70 75 80

Tyr Ser Gln Ile Glu Ala Ala Gly Ile Thr Tyr Ile Ser Val Gly His

85 90 95

Arg Lys Thr Leu His Arg Phe His Asn Lys Val Leu Tyr Ile Ser Lys

100 105 110

Ser Asp Ser Ala Thr Gly Ser Leu Arg Asn Trp Glu Leu Lys Pro Thr

115 120 125

Asp Gln Lys Ser Ile Glu Glu Ser Ser Pro Phe Ala Ser

130 135 140

<210> SEQ ID NO 185

›SEQUENCE LISTING · 48 of 53

<211> LENGTH: 705

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 185

gcacgagaaa cggggataac tggagcgtgg gacagcgcca acttattgca ctgggtaggg 60

cgctgctcaa gcaggcaaaa attttggtac tcgatgaggc gacagcatct gtcgacacag 120

caacagacaa tcttatccaa aagatcatcc gcagtgaatt caaggactgc acagtctgta 180

ccattgctca ccgtattccc accgttattg acagtgacct tgttctggtc cttagtgatg 240

gtaaaatcgc agagttcgac acgccccaga ggcttttaga ggacaagtca tctatgttca 300

tacagctagt atcggaatac tccactcggt cgagctgtat atagagaggc ttagcttaaa 360

accccgcccc aaacctggca acagaggctg ggaggcaaat agcccgtatc tgccatgctt 420

gcgccataga ggtccctgcg aacaccggag ggcggcgtag aagacgaggt gtacatgagt 480

gggaggaaca ctgggcgttc cctgacctga ataccgtgga atcggcgagg gagcgcggtt 540

ggtattggta ggcaccaggg gaggagttgg tgacactagt acattacccg aagctgatgc 600

ttcagtatgt atgtataaca acaatgcata ctgcttctcc ctttgcagag tggagaacca 660

agggaataac tcgtgcgtaa taagaggaga aatatttgtt ttttg 705

<210> SEQ ID NO 186

<211> LENGTH: 113

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 186

Thr Arg Asn Gly Asp Asn Trp Ser Val Gly Gln Arg Gln Leu Ile Ala

1 5 10 15

Leu Gly Arg Ala Leu Leu Lys Gln Ala Lys Ile Leu Val Leu Asp Glu

20 25 30

Ala Thr Ala Ser Val Asp Thr Ala Thr Asp Asn Leu Ile Gln Lys Ile

35 40 45

Ile Arg Ser Glu Phe Lys Asp Cys Thr Val Cys Thr Ile Ala His Arg

50 55 60

Ile Pro Thr Val Ile Asp Ser Asp Leu Val Leu Val Leu Ser Asp Gly

65 70 75 80

Lys Ile Ala Glu Phe Asp Thr Pro Gln Arg Leu Leu Glu Asp Lys Ser

85 90 95

Ser Met Phe Ile Gln Leu Val Ser Glu Tyr Ser Thr Arg Ser Ser Cys

100 105 110

Ile

<210> SEQ ID NO 187

<211> LENGTH: 845

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 187

gcacgagtcg aactggagca tgggtcaaag gcagcttttt tgtttgggac gtgcacttct 60

aagaagatgc cgtatcttag ttcttgatga ggccacggca tctatagaca acgctactga 120

tgctatcctt cagaagacga tcaggacaga gtttagagat tgcactgtca tcactgtcgc 180

acaccgtata cccaccgtta tggactgcga tatggtactt gcaatgagtg acgggaaagt 240

agtggagtac gacaagccta caaagctcgt ggaaaccgaa ggatctctcg tccgtgatct 300

tgtcaaggag tactggtcat acacatcgaa ttgaaatatc taggagaaac tgtgacaatc 360

acatgtccac tcgttgcagt ctattaaaaa agagataaca ggagcctgtt ctcacacaca 420

tccgatgaac tattctatct caagtccgaa gtagaggtgt caggcattgt cgggaagtgc 480

ttttgttaac tagttgaaca gggggaaacc tgtcgtccct taaaaaaagg aaacctccag 540

cgtttttttg ctccgatatt gagcgaataa accgaaatat ctagtggaag gcgcatcaag 600

tatgcatcct tgatactgca tgttcacaga aatgcttgtg cggcaggctg ttccaaggct 660

ttaaacttta tggtgcttta cattcctaat aaaccgaatt gtgtatatga tgtttgggaa 720

aatggtgatt tttcaggttt cagctccctc tcttgctcac gtttaatgta ttatcagtgt 780

aaattcatcc gtgattggat ttaaggcaac caatttgttg gtacgtaaaa aaaaaaaaaa 840

aaaag 845

<210> SEQ ID NO 188

<211> LENGTH: 110

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 188

His Glu Ser Asn Trp Ser Met Gly Gln Arg Gln Leu Phe Cys Leu Gly

1 5 10 15

Arg Ala Leu Leu Arg Arg Cys Arg Ile Leu Val Leu Asp Glu Ala Thr

20 25 30

Ala Ser Ile Asp Asn Ala Thr Asp Ala Ile Leu Gln Lys Thr Ile Arg

35 40 45

Thr Glu Phe Arg Asp Cys Thr Val Ile Thr Val Ala His Arg Ile Pro

50 55 60

Thr Val Met Asp Cys Asp Met Val Leu Ala Met Ser Asp Gly Lys Val

65 70 75 80

Val Glu Tyr Asp Lys Pro Thr Lys Leu Val Glu Thr Glu Gly Ser Leu

85 90 95

Val Arg Asp Leu Val Lys Glu Tyr Trp Ser Tyr Thr Ser Asn

100 105 110

<210> SEQ ID NO 189

<211> LENGTH: 247

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<400> SEQUENCE: 189

gcacgagctt cttcgacacc acgccgtccg gcaggatcct cagcagggcg tcgtcagatc 60

agacgaacgt cgatctcttc ctgccgttct tcgtctggat gagcgtatcc atgtacatca 120

ccgtgatcag tgtgctgatc gtcacgtgtc aggtggcgtg gccgtccgtc attgccataa 180

tccctctggt gatactgaac atctggtacc ggggctatta cttgtcgaca tcgagggagc 240

tgactcg 247

<210> SEQ ID NO 190

<211> LENGTH: 81

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<400> SEQUENCE: 190

Thr Ser Phe Phe Asp Thr Thr Pro Ser Gly Arg Ile Leu Ser Arg Ala

1 5 10 15

Ser Ser Asp Gln Thr Asn Val Asp Leu Phe Leu Pro Phe Phe Val Trp

20 25 30

Met Ser Val Ser Met Tyr Ile Thr Val Ile Ser Val Leu Ile Val Thr

35 40 45

Cys Gln Val Ala Trp Pro Ser Val Ile Ala Ile Ile Pro Leu Val Ile

50 55 60

Leu Asn Ile Trp Tyr Arg Gly Tyr Tyr Leu Ser Thr Ser Arg Glu Leu

65 70 75 80

Thr

<210> SEQ ID NO 191

<211> LENGTH: 1518

<212> TYPE: DNA

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: unsure

<222> LOCATION: (114)

<221> NAME/KEY: unsure

<222> LOCATION: (123)

<221> NAME/KEY: unsure

<222> LOCATION: (138)

<400> SEQUENCE: 191

acagatcaaa gcacagtgga tacaagaatt tttgacctga tgggctatct tctatttcct 60

gctattgaaa ttctaggaac agttattctg atgtcacatg ttgcatggca agtnttcata 120

gtnttaatcc ccattatnac tgcatcgctg tggtatcagc aatactacat agatgcagct 180

agagagttgc aaaggttggt tggagtttgc aggtcccccg tgcttcaaca tttttctgaa 240

tcaatggctg gctcaaatat aattagatgc tttcaaaagg aaagacaatt tatccgttat 300

attggatatt tggtggataa tttatctcga ccaagtttgt ataatgcagc cgcaatggaa 360

tggttgtgct ttcgtttgga tatgctttca tcctttgttt tttccttcac attgatactt 420

ctggtctctt caccatctgc tttaattgac ccaagtaaga ctgctggtct tgcggtcact 480

tatggactaa gcctcaatat gctacaaggg tgggctattg ctgtcctctg cagtttggaa 540

aatagaatga tatcagtgga aagaatgttg cagtatacga ctattccttc agaaccacca 600

cttacaatat cagaacgcca accaaataga cagtggccaa caaagggcga aattaagttt 660

cttaacctcc atgttcgata tgcaccgcag ctgccttttg ttctaaaagg cctgacgtgc 720

accttactcg gaggaaagaa gacaggtatt gttggtagaa caggtggtgg caaatcaacc 780

ttgattcagg cactatttcg cattgtggac ccatgtattg gccaagtgtt tatagatggc 840

actgacatct gcaccattgg actacatgac ctccggacaa gattgagcat cattccacaa 900

gatcctgtta tgtttgaggg gaccctgcga accaatattg acccccttgg ggagtacagt 960

gacgagaaaa tctgggaggc attggattca tgtcatcttg gggatgaagt tagaaagaac 1020

gagcttaagc ttgactctac agtgacagaa aggggcaaaa actggagcac aggtcagcgt 1080

caacttgtgt gtctgggaag ggtgattctg aaacggagga agatcttggt tttggatgag 1140

gcaacttcat ccgtggatcc aattacagac agcttgattc aaaaaacctt aaagaacctt 1200

aaagtcttgc ttctggataa cggtgaaatt gcagagcatg acgccccagc aaaactgcta 1260

gaggacagtt catccctatt ctccaaactt gtatctgagt ataccatggg atctgataag 1320

tggcgaatgc aagaaagcag ctaaatcaat actctacaga taatacagga caaacgccct 1380

aggaagtgtg agtatctacg tgttaccaat tttatagctt aataaaaaga ttttttttta 1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaagaaaaa aaaaagaaaa aaattaaaaa 1500

aaagaaaaaa aaaaaaaa 1518

<210> SEQ ID NO 192

<211> LENGTH: 447

›SEQUENCE LISTING · 49 of 53

<212> TYPE: PRT

<213> ORGANISM: Zea mays

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (46)

<400> SEQUENCE: 192

Thr Asp Gln Ser Thr Val Asp Thr Arg Ile Phe Asp Leu Met Gly Tyr

1 5 10 15

Leu Leu Phe Pro Ala Ile Glu Ile Leu Gly Thr Val Ile Leu Met Ser

20 25 30

His Val Ala Trp Gln Val Phe Ile Val Leu Ile Pro Ile Xaa Thr Ala

35 40 45

Ser Leu Trp Tyr Gln Gln Tyr Tyr Ile Asp Ala Ala Arg Glu Leu Gln

50 55 60

Arg Leu Val Gly Val Cys Arg Ser Pro Val Leu Gln His Phe Ser Glu

65 70 75 80

Ser Met Ala Gly Ser Asn Ile Ile Arg Cys Phe Gln Lys Glu Arg Gln

85 90 95

Phe Ile Arg Tyr Ile Gly Tyr Leu Val Asp Asn Leu Ser Arg Pro Ser

100 105 110

Leu Tyr Asn Ala Ala Ala Met Glu Trp Leu Cys Phe Arg Leu Asp Met

115 120 125

Leu Ser Ser Phe Val Phe Ser Phe Thr Leu Ile Leu Leu Val Ser Ser

130 135 140

Pro Ser Ala Leu Ile Asp Pro Ser Lys Thr Ala Gly Leu Ala Val Thr

145 150 155 160

Tyr Gly Leu Ser Leu Asn Met Leu Gln Gly Trp Ala Ile Ala Val Leu

165 170 175

Cys Ser Leu Glu Asn Arg Met Ile Ser Val Glu Arg Met Leu Gln Tyr

180 185 190

Thr Thr Ile Pro Ser Glu Pro Pro Leu Thr Ile Ser Glu Arg Gln Pro

195 200 205

Asn Arg Gln Trp Pro Thr Lys Gly Glu Ile Lys Phe Leu Asn Leu His

210 215 220

Val Arg Tyr Ala Pro Gln Leu Pro Phe Val Leu Lys Gly Leu Thr Cys

225 230 235 240

Thr Leu Leu Gly Gly Lys Lys Thr Gly Ile Val Gly Arg Thr Gly Gly

245 250 255

Gly Lys Ser Thr Leu Ile Gln Ala Leu Phe Arg Ile Val Asp Pro Cys

260 265 270

Ile Gly Gln Val Phe Ile Asp Gly Thr Asp Ile Cys Thr Ile Gly Leu

275 280 285

His Asp Leu Arg Thr Arg Leu Ser Ile Ile Pro Gln Asp Pro Val Met

290 295 300

Phe Glu Gly Thr Leu Arg Thr Asn Ile Asp Pro Leu Gly Glu Tyr Ser

305 310 315 320

Asp Glu Lys Ile Trp Glu Ala Leu Asp Ser Cys His Leu Gly Asp Glu

325 330 335

Val Arg Lys Asn Glu Leu Lys Leu Asp Ser Thr Val Thr Glu Arg Gly

340 345 350

Lys Asn Trp Ser Thr Gly Gln Arg Gln Leu Val Cys Leu Gly Arg Val

355 360 365

Ile Leu Lys Arg Arg Lys Ile Leu Val Leu Asp Glu Ala Thr Ser Ser

370 375 380

Val Asp Pro Ile Thr Asp Ser Leu Ile Gln Lys Thr Leu Lys Asn Leu

385 390 395 400

Lys Val Leu Leu Leu Asp Asn Gly Glu Ile Ala Glu His Asp Ala Pro

405 410 415

Ala Lys Leu Leu Glu Asp Ser Ser Ser Leu Phe Ser Lys Leu Val Ser

420 425 430

Glu Tyr Thr Met Gly Ser Asp Lys Trp Arg Met Gln Glu Ser Ser

435 440 445

<210> SEQ ID NO 193

<211> LENGTH: 2938

<212> TYPE: DNA

<213> ORGANISM: Oryza sativa

<400> SEQUENCE: 193

gcacgaggtt ctaacgatca aggtgtcgct ggaccgcatc ggcaagttcc tcatggagga 60

ggagttccgg gacgacgccg tcctgccatt accaatgcca agctccgaca tgatcaccat 120

ggccatcaac aatggcgtct tcagctggga gcctagcaag gccattgcaa cactgaaatc 180

catcagcatc gcagcaatgc agggcgagaa gatcgccgtc tgcggtccgg ttggcgcggg 240

gaaatcgtca ttgctctgcg caatgctcgg agaaatacca agaatgtctg gatcagtagc 300

tatgtccggc tcgatcgcct atgtcccgca gacgccatgg atccagagcg gcacggtgcg 360

cgacaacatc ctattcggga agccgatgaa caatgaggag tatgacaggg ccatcaggtg 420

ctgtgcattg gacaaggaca tggagaattt cccccatggc gacctgacgg agatcgggca 480

acgaggcctg aacatgagtg gagggcagaa gcagaggatt cagcttgcga gagccgtcta 540

caatggcgcc gacgtctacc tccttgacga tcctttcagc gccgtcgatg cgcataccgc 600

tgcgacactg ttcaatgatt gtgtcatggc agctcttgag aacaagacgg tcattcttgt 660

cacacatcaa gttgagttcc tctccaaggt tgacaagatc ctggtcatgg aaaatgggga 720

gataactcag gagggaactt acagtgagct tctgcaatcc ggcacggcat tcgaacagct 780

tgtgaatgcg cacaaggact caaaaacaat actagacacc gatgatcgcc gagaaggagc 840

aaaagaactc ggcgcgtttc agtaccaggt accactgata cagcagaaca gtgaggcaga 900

gatctcaacc ggtaacctga aatcagtcca gctgacagaa gaggagagga gggagctagg 960

agacattggg ctgaagccat acaaggatta tgtctcggta tccaagggct ggttcctcct 1020

ttccatgata ctagtcacgc agtgcgcgtt cttcggcctg caatgcctgg caacctactg 1080

gcttgcagta gcaattcaga accaacaatt cagtgctggg gttgtgattg gagtctatgc 1140

ggtgatggca actgtaagct gcttgtttgc atatgttagg agcctcattg ccgcgcattt 1200

tggactgaaa gcatcaaggg agttcttctc caggttcatg gattcggtgt ttaaggcacc 1260

catggtgttc tttgattcca ctcccacagg gagaatcatg accagggctt catcagattt 1320

gagcatctta gactttgaca tcccgtttgc aatgactttc gtgatctccg gctcgatcga 1380

aatagcaaca accatagcta tcatgatctt ggttacatgg cagcttgtac tggttgctat 1440

tcctgtcata gttgcacttc tgtacatcca gagatactac attgcctcag caagggagct 1500

ggtgaggatc aatggaacta caaaggcacc ggtcatgaac tacgcagcgg agtcgatgct 1560

gggagtgatc accataagag cctttgctga gacaaagagg tttattcaga caaatcttca 1620

gctcatcgac acagatgcga cactgttctt ctacacaaac gcagcactgg aatgggttct 1680

tttgcgcgtc gaggctctac agatcttggt tattgttgca tcatccatcc tcctcgtctt 1740

gcttccggag ggagcagttg ctccaggatt tcttggcctg tgtctctcgt atgcgttaat 1800

gctttcttca gcccaagtgt ttgtgacaag attttactca aacctggaaa actacatcat 1860

atctgtggag agaatcaaac agttcatgca tctgccagct gagcctcctg ctgttattac 1920

tgacagaagc cctccccctt catggccaac tgcaggaagg atagaattgg agaacttaag 1980

agtcaagtac ggcgaaatgc gcctacggta ttacgcggga ttacctgcac atttgcagca 2040

ggacataaga ttggagttgt tggaagaacc gggagcggga agacgactct tctgagcaca 2100

ttgttccgcc ttatcgaccc gtacagcggg agaattctca tcgatgatct tgacatttgc 2160

accatagggt tgaaagacct gaggatgaag ctcagcatca tccctcagga gccaacactt 2220

ttcagaggca gtgtaaggag caatgttgat cctctgggtc tgcacactga tgaagacatt 2280

tgggaggctt taaataagtg tcagttgaag aagacaatca gtgcccttcc tggccttctt 2340

gaatcaccag tgagcgacga cggcgagaac tggagcgccg gacaacggca gctcttctgc 2400

ctggcccggg tcctcctccg caggaacaag atcctggtcc tcgacgaggc gacggcgtcc 2460

atcgactcgg ccaccgacgc cgtcctgcag agagtcatca agcaggagtt ctcagggtgc 2520

actgtgatca ccatagctca cagggttccc actgtcactg acagtgacat ggtcatggtt 2580

ctttcatacg ggaaactcat agagtatgat aggccatcaa ggctgatgga gaatgaggac 2640

tctcccttct gtaagcttgt agctgagtac tggtccaact atagctgaat ctgccaagaa 2700

aaacggtcag gaacatcaaa tgcagaagac cttcactgct ttattcttca gtgaataagt 2760

ggaactggaa actgggacat tgcattgttg gccaatgatc atccagatgg acatatatga 2820

tgcactaatg atctggaaag tatgtatgag gggagctcat tcaaaaccag gggttcatgc 2880

aatgaaattg caagccttct cccaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 2938

<210> SEQ ID NO 194

<211> LENGTH: 895

<212> TYPE: PRT

<213> ORGANISM: Oryza sativa

<220> FEATURE:

<221> NAME/KEY: UNSURE

<222> LOCATION: (666)

<400> SEQUENCE: 194

His Glu Val Leu Thr Ile Lys Val Ser Leu Asp Arg Ile Gly Lys Phe

1 5 10 15

Leu Met Glu Glu Glu Phe Arg Asp Asp Ala Val Leu Pro Leu Pro Met

20 25 30

Pro Ser Ser Asp Met Ile Thr Met Ala Ile Asn Asn Gly Val Phe Ser

›SEQUENCE LISTING · 50 of 53

35 40 45

Trp Glu Pro Ser Lys Ala Ile Ala Thr Leu Lys Ser Ile Ser Ile Ala

50 55 60

Ala Met Gln Gly Glu Lys Ile Ala Val Cys Gly Pro Val Gly Ala Gly

65 70 75 80

Lys Ser Ser Leu Leu Cys Ala Met Leu Gly Glu Ile Pro Arg Met Ser

85 90 95

Gly Ser Val Ala Met Ser Gly Ser Ile Ala Tyr Val Pro Gln Thr Pro

100 105 110

Trp Ile Gln Ser Gly Thr Val Arg Asp Asn Ile Leu Phe Gly Lys Pro

115 120 125

Met Asn Asn Glu Glu Tyr Asp Arg Ala Ile Arg Cys Cys Ala Leu Asp

130 135 140

Lys Asp Met Glu Asn Phe Pro His Gly Asp Leu Thr Glu Ile Gly Gln

145 150 155 160

Arg Gly Leu Asn Met Ser Gly Gly Gln Lys Gln Arg Ile Gln Leu Ala

165 170 175

Arg Ala Val Tyr Asn Gly Ala Asp Val Tyr Leu Leu Asp Asp Pro Phe

180 185 190

Ser Ala Val Asp Ala His Thr Ala Ala Thr Leu Phe Asn Asp Cys Val

195 200 205

Met Ala Ala Leu Glu Asn Lys Thr Val Ile Leu Val Thr His Gln Val

210 215 220

Glu Phe Leu Ser Lys Val Asp Lys Ile Leu Val Met Glu Asn Gly Glu

225 230 235 240

Ile Thr Gln Glu Gly Thr Tyr Ser Glu Leu Leu Gln Ser Gly Thr Ala

245 250 255

Phe Glu Gln Leu Val Asn Ala His Lys Asp Ser Lys Thr Ile Leu Asp

260 265 270

Thr Asp Asp Arg Arg Glu Gly Ala Lys Glu Leu Gly Ala Phe Gln Tyr

275 280 285

Gln Val Pro Leu Ile Gln Gln Asn Ser Glu Ala Glu Ile Ser Thr Gly

290 295 300

Asn Leu Lys Ser Val Gln Leu Thr Glu Glu Glu Arg Arg Glu Leu Gly

305 310 315 320

Asp Ile Gly Leu Lys Pro Tyr Lys Asp Tyr Val Ser Val Ser Lys Gly

325 330 335

Trp Phe Leu Leu Ser Met Ile Leu Val Thr Gln Cys Ala Phe Phe Gly

340 345 350

Leu Gln Cys Leu Ala Thr Tyr Trp Leu Ala Val Ala Ile Gln Asn Gln

355 360 365

Gln Phe Ser Ala Gly Val Val Ile Gly Val Tyr Ala Val Met Ala Thr

370 375 380

Val Ser Cys Leu Phe Ala Tyr Val Arg Ser Leu Ile Ala Ala His Phe

385 390 395 400

Gly Leu Lys Ala Ser Arg Glu Phe Phe Ser Arg Phe Met Asp Ser Val

405 410 415

Phe Lys Ala Pro Met Val Phe Phe Asp Ser Thr Pro Thr Gly Arg Ile

420 425 430

Met Thr Arg Ala Ser Ser Asp Leu Ser Ile Leu Asp Phe Asp Ile Pro

435 440 445

Phe Ala Met Thr Phe Val Ile Ser Gly Ser Ile Glu Ile Ala Thr Thr

450 455 460

Ile Ala Ile Met Ile Leu Val Thr Trp Gln Leu Val Leu Val Ala Ile

465 470 475 480

Pro Val Ile Val Ala Leu Leu Tyr Ile Gln Arg Tyr Tyr Ile Ala Ser

485 490 495

Ala Arg Glu Leu Val Arg Ile Asn Gly Thr Thr Lys Ala Pro Val Met

500 505 510

Asn Tyr Ala Ala Glu Ser Met Leu Gly Val Ile Thr Ile Arg Ala Phe

515 520 525

Ala Glu Thr Lys Arg Phe Ile Gln Thr Asn Leu Gln Leu Ile Asp Thr

530 535 540

Asp Ala Thr Leu Phe Phe Tyr Thr Asn Ala Ala Leu Glu Trp Val Leu

545 550 555 560

Leu Arg Val Glu Ala Leu Gln Ile Leu Val Ile Val Ala Ser Ser Ile

565 570 575

Leu Leu Val Leu Leu Pro Glu Gly Ala Val Ala Pro Gly Phe Leu Gly

580 585 590

Leu Cys Leu Ser Tyr Ala Leu Met Leu Ser Ser Ala Gln Val Phe Val

595 600 605

Thr Arg Phe Tyr Ser Asn Leu Glu Asn Tyr Ile Ile Ser Val Glu Arg

610 615 620

Ile Lys Gln Phe Met His Leu Pro Ala Glu Pro Pro Ala Val Ile Thr

625 630 635 640

Asp Arg Ser Pro Pro Pro Ser Trp Pro Thr Ala Gly Arg Ile Glu Leu

645 650 655

Glu Asn Leu Arg Val Lys Tyr Gly Glu Xaa Ala Pro Thr Val Leu Arg

660 665 670

Gly Ile Thr Cys Thr Phe Ala Ala Gly His Lys Ile Gly Val Val Gly

675 680 685

Arg Thr Gly Ser Gly Lys Thr Thr Leu Leu Ser Thr Leu Phe Arg Leu

690 695 700

Ile Asp Pro Tyr Ser Gly Arg Ile Leu Ile Asp Asp Leu Asp Ile Cys

705 710 715 720

Thr Ile Gly Leu Lys Asp Leu Arg Met Lys Leu Ser Ile Ile Pro Gln

725 730 735

Glu Pro Thr Leu Phe Arg Gly Ser Val Arg Ser Asn Val Asp Pro Leu

740 745 750

Gly Leu His Thr Asp Glu Asp Ile Trp Glu Ala Leu Asn Lys Cys Gln

755 760 765

Leu Lys Lys Thr Ile Ser Ala Leu Pro Gly Leu Leu Glu Ser Pro Val

770 775 780

Ser Asp Asp Gly Glu Asn Trp Ser Ala Gly Gln Arg Gln Leu Phe Cys

785 790 795 800

Leu Ala Arg Val Leu Leu Arg Arg Asn Lys Ile Leu Val Leu Asp Glu

805 810 815

Ala Thr Ala Ser Ile Asp Ser Ala Thr Asp Ala Val Leu Gln Arg Val

820 825 830

Ile Lys Gln Glu Phe Ser Gly Cys Thr Val Ile Thr Ile Ala His Arg

835 840 845

Val Pro Thr Val Thr Asp Ser Asp Met Val Met Val Leu Ser Tyr Gly

850 855 860

Lys Leu Ile Glu Tyr Asp Arg Pro Ser Arg Leu Met Glu Asn Glu Asp

865 870 875 880

Ser Pro Phe Cys Lys Leu Val Ala Glu Tyr Trp Ser Asn Tyr Ser

885 890 895

<210> SEQ ID NO 195

<211> LENGTH: 1265

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 195

gcacgagccc atctcagagg atgtgcagcc acctgttgaa ggaacgactc ttatagcagt 60

ctatgttggt ttggccattg gaagttcttt ctgcatcctt gccagagcaa tacttcttgt 120

aacagctggt tataaaacag ctactatact cttcaataaa atgcacttct gcattttccg 180

tgctccaatg tcatttttcg attccactcc aagtggacga atccttaaca gagcttcgac 240

cgatcaaagt gcattggata cagacattcc ttatcaaatt gcatcatttg ccttcatttt 300

gatccagctt ctgggaatta tagcagtgat gtctcaagct gcatggcagg ttttcgttgt 360

ctttatacct gtgatagcaa tcagcgtctt gtatcagcaa tattatattc catcagcccg 420

agaactatca cgcttagttg gagtctgcaa agccccaatc attcaacact ttgctgaaac 480

aatttctggt actacaacta ttagaagctt tgatcagcag tcaagatttc aggaaacaaa 540

tatgaaactg actgatggat attctcggcc aatgttcaat attgctggtg ccgtggaatg 600

gttgtgtttc cgtttggata tgttgtcttc tatcacattt gccttttcct taatattctt 660

aatatctatt ccacagggat tcatagatcc aggccttgct ggtttagctg ttacatatgg 720

acttaatttg aacattgtac aaggttggat gatatggaat ctctgcaata tggagaacaa 780

aattatatca gtagaaagaa tacttcagta tacttgtatt ccttgtgagc cttcccttgt 840

tgtagacgat aatcggccag atccttcttg gccttcatat ggcgaggttg atatacaaga 900

tttgaaggtt cgttatgctc cacatctccc acttgtcttg cgcggtctta catgcaaatt 960

tcgtggagga ctgaaaactg gcattgttgg gagaacagga agtggtaaat ccactctcat 1020

acaaacactt ttccgaattg ttgagcctac tgccggccaa gttatgattg acagcatcaa 1080

catctcttca attggacttc atgatttgag gtctagacta agcatcattc ctcaagatcc 1140

aacaatgttt gaagggaccg tgagaaataa tttggacccc ctggaagaat acactgatga 1200

agaaatttgg gaggccttgg ataagtgtca acttggagat gaagttagaa aaaaaaaaaa 1260

aaaaa 1265

<210> SEQ ID NO 196

<211> LENGTH: 418

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 196

His Glu Pro Ile Ser Glu Asp Val Gln Pro Pro Val Glu Gly Thr Thr

1 5 10 15

Leu Ile Ala Val Tyr Val Gly Leu Ala Ile Gly Ser Ser Phe Cys Ile

20 25 30

Leu Ala Arg Ala Ile Leu Leu Val Thr Ala Gly Tyr Lys Thr Ala Thr

35 40 45

Ile Leu Phe Asn Lys Met His Phe Cys Ile Phe Arg Ala Pro Met Ser

50 55 60

Phe Phe Asp Ser Thr Pro Ser Gly Arg Ile Leu Asn Arg Ala Ser Thr

65 70 75 80

Asp Gln Ser Ala Leu Asp Thr Asp Ile Pro Tyr Gln Ile Ala Ser Phe

›SEQUENCE LISTING · 51 of 53

85 90 95

Ala Phe Ile Leu Ile Gln Leu Leu Gly Ile Ile Ala Val Met Ser Gln

100 105 110

Ala Ala Trp Gln Val Phe Val Val Phe Ile Pro Val Ile Ala Ile Ser

115 120 125

Val Leu Tyr Gln Gln Tyr Tyr Ile Pro Ser Ala Arg Glu Leu Ser Arg

130 135 140

Leu Val Gly Val Cys Lys Ala Pro Ile Ile Gln His Phe Ala Glu Thr

145 150 155 160

Ile Ser Gly Thr Thr Thr Ile Arg Ser Phe Asp Gln Gln Ser Arg Phe

165 170 175

Gln Glu Thr Asn Met Lys Leu Thr Asp Gly Tyr Ser Arg Pro Met Phe

180 185 190

Asn Ile Ala Gly Ala Val Glu Trp Leu Cys Phe Arg Leu Asp Met Leu

195 200 205

Ser Ser Ile Thr Phe Ala Phe Ser Leu Ile Phe Leu Ile Ser Ile Pro

210 215 220

Gln Gly Phe Ile Asp Pro Gly Leu Ala Gly Leu Ala Val Thr Tyr Gly

225 230 235 240

Leu Asn Leu Asn Ile Val Gln Gly Trp Met Ile Trp Asn Leu Cys Asn

245 250 255

Met Glu Asn Lys Ile Ile Ser Val Glu Arg Ile Leu Gln Tyr Thr Cys

260 265 270

Ile Pro Cys Glu Pro Ser Leu Val Val Asp Asp Asn Arg Pro Asp Pro

275 280 285

Ser Trp Pro Ser Tyr Gly Glu Val Asp Ile Gln Asp Leu Lys Val Arg

290 295 300

Tyr Ala Pro His Leu Pro Leu Val Leu Arg Gly Leu Thr Cys Lys Phe

305 310 315 320

Arg Gly Gly Leu Lys Thr Gly Ile Val Gly Arg Thr Gly Ser Gly Lys

325 330 335

Ser Thr Leu Ile Gln Thr Leu Phe Arg Ile Val Glu Pro Thr Ala Gly

340 345 350

Gln Val Met Ile Asp Ser Ile Asn Ile Ser Ser Ile Gly Leu His Asp

355 360 365

Leu Arg Ser Arg Leu Ser Ile Ile Pro Gln Asp Pro Thr Met Phe Glu

370 375 380

Gly Thr Val Arg Asn Asn Leu Asp Pro Leu Glu Glu Tyr Thr Asp Glu

385 390 395 400

Glu Ile Trp Glu Ala Leu Asp Lys Cys Gln Leu Gly Asp Glu Val Arg

405 410 415

Lys Lys

<210> SEQ ID NO 197

<211> LENGTH: 1412

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 197

gcacgagtgg acttgctgtg acatatggcc tgaatttaaa tgcacgtcta tcacggtgga 60

tactcagctt ttgcaaactt gaaaataaaa ttatatctat tgagagaatt tatcagtaca 120

gccaaattcc tagtgaagca cccacagtta ttgaagatta tcgccctcca tcctcatggc 180

ctgaaaatgg gacaattgaa ataattgatt tgaagattcg ttacaaggag aatcttcctt 240

tggtgcttta tggagtaaca tgcacatttc ctggtggaaa gaagattgga atagtaggac 300

gtactggcag tggaaaatct actttaattc aggcgttatt tcgattgatt gaaccaacaa 360

gtgggagtat ccttatagac aacattaata tttcagagat tggccttcat gaccttcgaa 420

gccatctcag tatcatacca caagatccaa ccttatttga aggtaccatt cgaggcaatc 480

ttgatcctct ggatgagcac tcagataaag agatttggga ggcacttgat aagtctcagc 540

ttggagaggt tatccgtgag aaaggacaac agcttgatac gccagttcta gaaaatggag 600

ataattggag tgtaggacag cgacaacttg ttgctctggg ccgagctctg ctgcagcagt 660

caagaatact tgtactagat gaagcaacag catcagttga taccgccaca gataatctta 720

tacagaagat tatccgaagt gagttcaaag aatgcactgt ttgcaccatt gcacatcgaa 780

tacctactgt cattgacagt gatctagttc ttgtgctcag tgatggtcga gttgcagagt 840

tcaacactcc ttcaagacta ttagaggata agtcatccat gtttctgaag ctggtgactg 900

agtactcatc acgttcaagt ggcataccag acttttagaa caaatggaag gtgtgaatgc 960

tttcatagtg tggtggctgg agcttaagat agttcaaaag ttgaatcagg aagtgatgcc 1020

acccttgcat gtcactgctg cattcggggc atgcatagag acacgagatg gaaacaaaca 1080

aaataaaagg gagaggtttg tgcctcctca tgaatcaagc atcctactgg gggaaatttg 1140

tttgattatt ccccttaaag ttgagaaatt catgcaaggt tagcatgctt tgtaacacaa 1200

aataagatga tctgtgatta caggaaagta acgaaatagt ttgtagaatg aggcactagg 1260

attttgcttg gttagaaaaa gtgtagagtt taaactagtt ttgtgtattc cacaattttc 1320

ttgtagtgaa agtttagaat taagccaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 1412

<210> SEQ ID NO 198

<211> LENGTH: 311

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 198

Thr Ser Gly Leu Ala Val Thr Tyr Gly Leu Asn Leu Asn Ala Arg Leu

1 5 10 15

Ser Arg Trp Ile Leu Ser Phe Cys Lys Leu Glu Asn Lys Ile Ile Ser

20 25 30

Ile Glu Arg Ile Tyr Gln Tyr Ser Gln Ile Pro Ser Glu Ala Pro Thr

35 40 45

Val Ile Glu Asp Tyr Arg Pro Pro Ser Ser Trp Pro Glu Asn Gly Thr

50 55 60

Ile Glu Ile Ile Asp Leu Lys Ile Arg Tyr Lys Glu Asn Leu Pro Leu

65 70 75 80

Val Leu Tyr Gly Val Thr Cys Thr Phe Pro Gly Gly Lys Lys Ile Gly

85 90 95

Ile Val Gly Arg Thr Gly Ser Gly Lys Ser Thr Leu Ile Gln Ala Leu

100 105 110

Phe Arg Leu Ile Glu Pro Thr Ser Gly Ser Ile Leu Ile Asp Asn Ile

115 120 125

Asn Ile Ser Glu Ile Gly Leu His Asp Leu Arg Ser His Leu Ser Ile

130 135 140

Ile Pro Gln Asp Pro Thr Leu Phe Glu Gly Thr Ile Arg Gly Asn Leu

145 150 155 160

Asp Pro Leu Asp Glu His Ser Asp Lys Glu Ile Trp Glu Ala Leu Asp

165 170 175

Lys Ser Gln Leu Gly Glu Val Ile Arg Glu Lys Gly Gln Gln Leu Asp

180 185 190

Thr Pro Val Leu Glu Asn Gly Asp Asn Trp Ser Val Gly Gln Arg Gln

195 200 205

Leu Val Ala Leu Gly Arg Ala Leu Leu Gln Gln Ser Arg Ile Leu Val

210 215 220

Leu Asp Glu Ala Thr Ala Ser Val Asp Thr Ala Thr Asp Asn Leu Ile

225 230 235 240

Gln Lys Ile Ile Arg Ser Glu Phe Lys Glu Cys Thr Val Cys Thr Ile

245 250 255

Ala His Arg Ile Pro Thr Val Ile Asp Ser Asp Leu Val Leu Val Leu

260 265 270

Ser Asp Gly Arg Val Ala Glu Phe Asn Thr Pro Ser Arg Leu Leu Glu

275 280 285

Asp Lys Ser Ser Met Phe Leu Lys Leu Val Thr Glu Tyr Ser Ser Arg

290 295 300

Ser Ser Gly Ile Pro Asp Phe

305 310

<210> SEQ ID NO 199

<211> LENGTH: 1936

<212> TYPE: DNA

<213> ORGANISM: Glycine max

<400> SEQUENCE: 199

gcacgagcta gcatggattt tatccttctt ggctggtgac tactggctag caattggaac 60

tgcagaagat agtgcctttc ctccttctac attcatcatt gtctatgctt gcatagcagg 120

tcttgtatgt actgtggtga tgatcagatc agtcttattt acatattggg gtctaaagac 180

atctcaaagc ttcttcagtg gaatgcttga aagcatcttg catgcaccaa tgtcattctt 240

tgacacaact ccctctggca gaattttgag tcgtgtatct actgatatac tttgggttga 300

tatctcaatt ccaatgttgg tgaacttcgt catggttgct tacttctcag taaccagcat 360

cctcattgta acatgccaaa atgcttggga aacggtcttc ctcttaatcc cactgttctg 420

gctgaataac tggtatcgga aatattacct tgcatcttct cgggaattga ctcgccttga 480

ttccatcacc aaagctcctg tgattcacca tttttcagaa accattgctg gtgttatgac 540

catacgcggc ttcagaaagc agaacgcatt ttgtcaagaa aatattgaca aagtgaatgc 600

tagtctgaga atggatttcc acaacaacgg tgctaatgaa tggcttggtt ttcgcttgga 660

ctatatggga gtgattttcc tttgctttgc cactattttt atgatctttc tgccaagtgc 720

tattattaag ccagaatatg ttggtttgtc tttgtcctat ggcctggctc tcagtagtct 780

cttggcattt accataagca tgacttgttc tgttgagaac aaaatggttt cagttgagag 840

aataaagcag ttctccagcc ttccctcaga agctccatgg aaaattgctg acaaaactcc 900

tcctcagaat tggcccagtc aaggcattat tgagttaacc aatttgcagg ttcggtatcg 960

accaaatact cctctggttc tcaagggaat ctctctcacc attgaagcag gagaaaagat 1020

›SEQUENCE LISTING · 52 of 53

tggtgttgtt ggtcgtacag gaagtgggaa atcaaccctc attcaggtgt tatttaggtt 1080

gattgagcct tcagctggga aaataactgt tgatggaatc aacatttgca ccctgggcct 1140

tcatgatgtg aggtctagat ttggaattat tcctcaagag cctgtcctct ttcaaggaac 1200

agtacgaagc aacattgacc cccttggact gtattcagag gaagaaattt ggaagagtct 1260

tgagcgctgc caactgaaag atgtggtggc tgcaaagcca gagaaacttg aggctccagt 1320

ggttgatggt ggagacaatt ggagtgtggg gcaaagacag cttctttgct tgggaaggat 1380

catgctaaaa agcagcaaaa tattgttcat ggatgaggca acagcatccg ttgattcgca 1440

aactgacgct gtgatacaaa agatcatccg tgaggacttt gcggatcgta caattatcag 1500

cattgctcat agaataccaa cagtcatgga ttgtgacagg gttttagtca ttgatgcagg 1560

ctatgcaaag gaatatgaca agccatcgcg tttgctagaa aggcattcac tttttggagc 1620

attggttaag gagtactcta atagatcagc tgaactagag gtttagattc taacaaggac 1680

ccagttgatg cactcagttt ccatccaaca cacagcacca aagtcaaaag acatgagtcg 1740

atgggtctat tgagaaatca atatttttga gcattagttt tgatgtttta ctctttcagt 1800

agtttaataa ttagactagg acaataggaa ggaaatttta aaatttttta ctaataaaaa 1860

atcaagtttg ggtgcaattg ttactatttt ataaaaaaaa aaaaaaaatc tgtttgctaa 1920

aaaaaaaaaa aaaaaa 1936

<210> SEQ ID NO 200

<211> LENGTH: 554

<212> TYPE: PRT

<213> ORGANISM: Glycine max

<400> SEQUENCE: 200

His Glu Leu Ala Trp Ile Leu Ser Phe Leu Ala Gly Asp Tyr Trp Leu

1 5 10 15

Ala Ile Gly Thr Ala Glu Asp Ser Ala Phe Pro Pro Ser Thr Phe Ile

20 25 30

Ile Val Tyr Ala Cys Ile Ala Gly Leu Val Cys Thr Val Val Met Ile

35 40 45

Arg Ser Val Leu Phe Thr Tyr Trp Gly Leu Lys Thr Ser Gln Ser Phe

50 55 60

Phe Ser Gly Met Leu Glu Ser Ile Leu His Ala Pro Met Ser Phe Phe

65 70 75 80

Asp Thr Thr Pro Ser Gly Arg Ile Leu Ser Arg Val Ser Thr Asp Ile

85 90 95

Leu Trp Val Asp Ile Ser Ile Pro Met Leu Val Asn Phe Val Met Val

100 105 110

Ala Tyr Phe Ser Val Thr Ser Ile Leu Ile Val Thr Cys Gln Asn Ala

115 120 125

Trp Glu Thr Val Phe Leu Leu Ile Pro Leu Phe Trp Leu Asn Asn Trp

130 135 140

Tyr Arg Lys Tyr Tyr Leu Ala Ser Ser Arg Glu Leu Thr Arg Leu Asp

145 150 155 160

Ser Ile Thr Lys Ala Pro Val Ile His His Phe Ser Glu Thr Ile Ala

165 170 175

Gly Val Met Thr Ile Arg Gly Phe Arg Lys Gln Asn Ala Phe Cys Gln

180 185 190

Glu Asn Ile Asp Lys Val Asn Ala Ser Leu Arg Met Asp Phe His Asn

195 200 205

Asn Gly Ala Asn Glu Trp Leu Gly Phe Arg Leu Asp Tyr Met Gly Val

210 215 220

Ile Phe Leu Cys Phe Ala Thr Ile Phe Met Ile Phe Leu Pro Ser Ala

225 230 235 240

Ile Ile Lys Pro Glu Tyr Val Gly Leu Ser Leu Ser Tyr Gly Leu Ala

245 250 255

Leu Ser Ser Leu Leu Ala Phe Thr Ile Ser Met Thr Cys Ser Val Glu

260 265 270

Asn Lys Met Val Ser Val Glu Arg Ile Lys Gln Phe Ser Ser Leu Pro

275 280 285

Ser Glu Ala Pro Trp Lys Ile Ala Asp Lys Thr Pro Pro Gln Asn Trp

290 295 300

Pro Ser Gln Gly Ile Ile Glu Leu Thr Asn Leu Gln Val Arg Tyr Arg

305 310 315 320

Pro Asn Thr Pro Leu Val Leu Lys Gly Ile Ser Leu Thr Ile Glu Ala

325 330 335

Gly Glu Lys Ile Gly Val Val Gly Arg Thr Gly Ser Gly Lys Ser Thr

340 345 350

Leu Ile Gln Val Leu Phe Arg Leu Ile Glu Pro Ser Ala Gly Lys Ile

355 360 365

Thr Val Asp Gly Ile Asn Ile Cys Thr Leu Gly Leu His Asp Val Arg

370 375 380

Ser Arg Phe Gly Ile Ile Pro Gln Glu Pro Val Leu Phe Gln Gly Thr

385 390 395 400

Val Arg Ser Asn Ile Asp Pro Leu Gly Leu Tyr Ser Glu Glu Glu Ile

405 410 415

Trp Lys Ser Leu Glu Arg Cys Gln Leu Lys Asp Val Val Ala Ala Lys

420 425 430

Pro Glu Lys Leu Glu Ala Pro Val Val Asp Gly Gly Asp Asn Trp Ser

435 440 445

Val Gly Gln Arg Gln Leu Leu Cys Leu Gly Arg Ile Met Leu Lys Ser

450 455 460

Ser Lys Ile Leu Phe Met Asp Glu Ala Thr Ala Ser Val Asp Ser Gln

465 470 475 480

Thr Asp Ala Val Ile Gln Lys Ile Ile Arg Glu Asp Phe Ala Asp Arg

485 490 495

Thr Ile Ile Ser Ile Ala His Arg Ile Pro Thr Val Met Asp Cys Asp

500 505 510

Arg Val Leu Val Ile Asp Ala Gly Tyr Ala Lys Glu Tyr Asp Lys Pro

515 520 525

Ser Arg Leu Leu Glu Arg His Ser Leu Phe Gly Ala Leu Val Lys Glu

530 535 540

Tyr Ser Asn Arg Ser Ala Glu Leu Glu Val

545 550

<210> SEQ ID NO 201

<211> LENGTH: 1328

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 201

ctcgtgccga attcggcacg aggtcacata tgggcttaac ttgaacatgc tgcaagcatg 60

ggttgtgtgg agcatgtgca atttggagaa caagatcata tcagtagaaa gaattctgca 120

atacataagc attcctgaag agcccccact ttcaatgtca gaagataagt tgccccataa 180

ctggccgtca cagggagaaa ttcagcttca tgacgtccat gtaagatatg ctccacaact 240

accatttgtt ctgaagggcc ttaacgtcac tttccctgga ggcatgaaga ctggtattgt 300

tggaagaaca ggcagtggta aatcaacact catacaggcc cttttccgta ttgtcgagcc 360

tactgtcggt cagatactgg tagatggtgt tgacatctgc accatcgggc tgcatgatct 420

gagatctaga cttagcatca ttccacaaga tccaacgatg tttgagggaa ctgtgaggag 480

caaccttgac cctcttaacg agtacaatga cgatcagatc tgggaggcct tggataactg 540

tcagctggga gatgaggtca ggaagaagga gctgaaactc gactcaccag tgatcgagaa 600

cggagagaac tggagcgtgg gccagcgcca gctagtctgt cttggaagag tgattctgaa 660

acggaccaag atactggttc tggacgaagc cactgcttca gtggataccg cgacagacaa 720

catgattcag aaaacactac gtgagaactt ctcggaggcg acggtcatca cgattgcgca 780

tcgaatcacc tcggtcctcg acagcgacat ggttctgctc ctcgacaacg gtgtggccgt 840

ggagcgtgac acgccggcca agctgctgga gaacaagtcg tctctgttct caaagcttgt 900

ggcggagtac acgatcagag cgacgcacgc gtagccaacg aatagatcct ccagcaaatt 960

cttccatgcc gttgaggctt cttgagtgag tctggccgtc gataatgccg aaaaattact 1020

ggtagatgtg atgtgaagtg ctctcggatc tgccacggtg ttggtgctac tgatacgcgc 1080

acggtaactt tacattttct gaaggaaaag gctgaaggtg gttaagagtc ggtcaaagtt 1140

tagtgtttga agaaagtagt tcgttaagga tagcaagacc tgaaccatgg tagtagtcac 1200

tcactttacg gcggaccata cacgccgaaa aagttttggg gcttagtaat ttgatgatgt 1260

actgtgtgat accgggataa ataaatgaag tggaagtggt tcagtttaaa aaaaaaaaaa 1320

aaaaaaaa 1328

<210> SEQ ID NO 202

<211> LENGTH: 310

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 202

Ser Cys Arg Ile Arg His Glu Val Thr Tyr Gly Leu Asn Leu Asn Met

1 5 10 15

Leu Gln Ala Trp Val Val Trp Ser Met Cys Asn Leu Glu Asn Lys Ile

20 25 30

Ile Ser Val Glu Arg Ile Leu Gln Tyr Ile Ser Ile Pro Glu Glu Pro

35 40 45

Pro Leu Ser Met Ser Glu Asp Lys Leu Pro His Asn Trp Pro Ser Gln

50 55 60

Gly Glu Ile Gln Leu His Asp Val His Val Arg Tyr Ala Pro Gln Leu

65 70 75 80

Pro Phe Val Leu Lys Gly Leu Asn Val Thr Phe Pro Gly Gly Met Lys

85 90 95

Thr Gly Ile Val Gly Arg Thr Gly Ser Gly Lys Ser Thr Leu Ile Gln

100 105 110

Ala Leu Phe Arg Ile Val Glu Pro Thr Val Gly Gln Ile Leu Val Asp

›SEQUENCE LISTING · 53 of 53

115 120 125

Gly Val Asp Ile Cys Thr Ile Gly Leu His Asp Leu Arg Ser Arg Leu

130 135 140

Ser Ile Ile Pro Gln Asp Pro Thr Met Phe Glu Gly Thr Val Arg Ser

145 150 155 160

Asn Leu Asp Pro Leu Asn Glu Tyr Asn Asp Asp Gln Ile Trp Glu Ala

165 170 175

Leu Asp Asn Cys Gln Leu Gly Asp Glu Val Arg Lys Lys Glu Leu Lys

180 185 190

Leu Asp Ser Pro Val Ile Glu Asn Gly Glu Asn Trp Ser Val Gly Gln

195 200 205

Arg Gln Leu Val Cys Leu Gly Arg Val Ile Leu Lys Arg Thr Lys Ile

210 215 220

Leu Val Leu Asp Glu Ala Thr Ala Ser Val Asp Thr Ala Thr Asp Asn

225 230 235 240

Met Ile Gln Lys Thr Leu Arg Glu Asn Phe Ser Glu Ala Thr Val Ile

245 250 255

Thr Ile Ala His Arg Ile Thr Ser Val Leu Asp Ser Asp Met Val Leu

260 265 270

Leu Leu Asp Asn Gly Val Ala Val Glu Arg Asp Thr Pro Ala Lys Leu

275 280 285

Leu Glu Asn Lys Ser Ser Leu Phe Ser Lys Leu Val Ala Glu Tyr Thr

290 295 300

Ile Arg Ala Thr His Ala

305 310

<210> SEQ ID NO 203

<211> LENGTH: 1069

<212> TYPE: DNA

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 203

tggtttaggc acgtggattt ggtacgggca ccgcctcgag cggagagatg atcaatgtcg 60

tgagcctcga tgccgagtgt gtaggagatt tcagtcgatc catgcacgat ctttggcggc 120

ttcctgtgca aattgttcta gccatgctca tcttatactc gacacttggt ttttgccctg 180

catttgctgc ccttcttgcc actgctttga caattggggg taataagcct ttagggagaa 240

tggagcaaaa ttatcaagag agaatgatga gcgccaagga cgtgagaatg agggctatgt 300

ctgagatctt acagagcatg cgcattctca agcttcaagg atgggaaatg atcttcttgt 360

ccaagatcat agagttgagg aaggtagaga tgaattggct aaagaagaac gtatacacct 420

cggcgatgct cctatccatc ttcttttctg ctcctgcttt tgttgctatg gtcactttcg 480

gtgtttgcgt gcttatgggc attccattag aaacaggtaa agtgctatgt gccctggcaa 540

catttagaca gctgcaaaca cctatccatg gtctaccaga cgcatactct atgatcattc 600

agacaaaagt atcgcttgac aggatatgct catttctctg tcttgaggaa ctccctagtg 660

atgttgtaac taagcttcct aggggtacca ccgatgtgtc gattgaggtg acgaacggtc 720

atttctcttg gaatacatct tctcaagtgc ctactctcca agacgtgaac ttccgtatac 780

gacagggaat gagggttgct gtctgtggaa cagttggatc tggcaaatca agtttattgt 840

cttgcatact cggtgagata ccaaaattat caggagaggt taaaacttgt ggcaggatct 900

cctatgttag ccaaacacct tggatacaga gtgggaaaat tgaagataat atacttttcg 960

gcacagaaat gaatagggag aggtatgaaa aggtccttga agcatgctca cttatcaagg 1020

acctggatat attaccattc ggtgaccaga cgattatagg agctcgtgc 1069

<210> SEQ ID NO 204

<211> LENGTH: 353

<212> TYPE: PRT

<213> ORGANISM: Triticum aestivum

<400> SEQUENCE: 204

Ala Arg Gly Phe Gly Thr Gly Thr Ala Ser Ser Gly Glu Met Ile Asn

1 5 10 15

Val Val Ser Leu Asp Ala Glu Cys Val Gly Asp Phe Ser Arg Ser Met

20 25 30

His Asp Leu Trp Arg Leu Pro Val Gln Ile Val Leu Ala Met Leu Ile

35 40 45

Leu Tyr Ser Thr Leu Gly Phe Cys Pro Ala Phe Ala Ala Leu Leu Ala

50 55 60

Thr Ala Leu Thr Ile Gly Gly Asn Lys Pro Leu Gly Arg Met Glu Gln

65 70 75 80

Asn Tyr Gln Glu Arg Met Met Ser Ala Lys Asp Val Arg Met Arg Ala

85 90 95

Met Ser Glu Ile Leu Gln Ser Met Arg Ile Leu Lys Leu Gln Gly Trp

100 105 110

Glu Met Ile Phe Leu Ser Lys Ile Ile Glu Leu Arg Lys Val Glu Met

115 120 125

Asn Trp Leu Lys Lys Asn Val Tyr Thr Ser Ala Met Leu Leu Ser Ile

130 135 140

Phe Phe Ser Ala Pro Ala Phe Val Ala Met Val Thr Phe Gly Val Cys

145 150 155 160

Val Leu Met Gly Ile Pro Leu Glu Thr Gly Lys Val Leu Cys Ala Leu

165 170 175

Ala Thr Phe Arg Gln Leu Gln Thr Pro Ile His Gly Leu Pro Asp Ala

180 185 190

Tyr Ser Met Ile Ile Gln Thr Lys Val Ser Leu Asp Arg Ile Cys Ser

195 200 205

Phe Leu Cys Leu Glu Glu Leu Pro Ser Asp Val Val Thr Lys Leu Pro

210 215 220

Arg Gly Thr Thr Asp Val Ser Ile Glu Val Thr Asn Gly His Phe Ser

225 230 235 240

Trp Asn Thr Ser Ser Gln Val Pro Thr Leu Gln Asp Val Asn Phe Arg

245 250 255

Ile Arg Gln Gly Met Arg Val Ala Val Cys Gly Thr Val Gly Ser Gly

260 265 270

Lys Ser Ser Leu Leu Ser Cys Ile Leu Gly Glu Ile Pro Lys Leu Ser

275 280 285

Gly Glu Val Lys Thr Cys Gly Arg Ile Ser Tyr Val Ser Gln Thr Pro

290 295 300

Trp Ile Gln Ser Gly Lys Ile Glu Asp Asn Ile Leu Phe Gly Thr Glu

305 310 315 320

Met Asn Arg Glu Arg Tyr Glu Lys Val Leu Glu Ala Cys Ser Leu Ile

325 330 335

Lys Asp Leu Asp Ile Leu Pro Phe Gly Asp Gln Thr Ile Ile Gly Ala

340 345 350

Arg

›Tables in the description — 4
TABLE 2 — cDNA Libraries from Corn, Rice, Soybean and Wheat
LibraryTissueClone
cbn10Corn developing kernel (embryo and endosperm); 10 dayscbn10.pk0055.e7
after pollinationcbn10.pk0023.h4
cbn10.pk0061.a4
cbn10.pk0015.b5
ccase-bCorn callus type II tissue, somatic embryo formed, highlyccase-b.pk0001.a3
transformableccase-b.pk0009.c11
cco1nCorn cob of 67 day old plants grown in green house*cco1n.pk084.i6
cco1.pk0035.d12
cco1.pk0004.f8
cco1.pk0025.b9
cen3nCorn endosperm 20 days after pollination*cen3n.pk0005.f9
cen3n.pk0045.f5
cen3n.pk0019.d10
cen3n.pk0196.g3
cen3n.pk0211.h10
cep7Corn 7 day old epicotyl; grown in lightcep7.pk0016.h9
chpc24Corn 8 day old shoot treated 24 hours with herbicide****chpc24.pk0004.b2
cpf1cCorn pooled BMS treated with chemicals related to proteincpf1c.pk003.15
synthesis******
cpi1cCorn pooled BMS treated with chemicals related tocpi1c.pk014.m16
biochemical compound synthesis*****
cpl1cCorn Pooled BMS Treated With Chemical Chelators***cpl1c.pk004.k22
cr1nCorn root from 7 day old seedlings*cr1n.pk0018.e6
cr1n.pk0147.b9
cr1n.pk0002.f7
cr1n.pk0002.a6
cr1n.pk0195.h6
cr1n.pk0176.b9
cs1Corn leaf sheath from 5 week old plantcs1.pk0038.d1
cs1.pk0080.b11
cs1.pk0051.e12
cs1.pk0067.d8
cta1Corn tasselcta1.pk0006.g8
cta1.pk0006.g8
p0014p0014.ctutf13r
p0014.ctutf13rb
p0031Corn shoot culturep0031.ccmar94r
p0031.ccmar94rx
p0044Corn pedicel 20 days after pollinationp0044.cjrai81r
p0059Corn scutelar node from seeds two and three days afterp0059.cmsba25r
germination
p0072Corn coleoptile 14 days after planting etiolated seedlingp0072.comgu73rb
p0080Corn pedical 10 days after pollinationp0080.cgabm66r
p0083Corn whole kernels 7 days after pollinationp0083.cldec87r
p0085Corn shoot culturep0085.cscag24r
p107Corn whole kernels 7 days after pollination*p0107.cbcas83r
p0110Corn (stages V3/V4)** leaf tissue minus midrib harvested 4p0110.cgsmr50r
hours, 24 hours and 7 days after infiltration with salicylicp0110.cgsnq75r
acid, pooled*p0110.cgsnq76r
p0117Expanding internode: plants sampled @ the V10 stage**.p0117.chclk47ra
Internodes 5-9 (the upper 4-5 expanding internodes)
p0119Corn V12-stage** ear shoot with husk, night harvested*p0119.cmtoh11r
p0119.cmtnf84r
p0121Corn shank ear tissue collected 5 days after pollination*p0121.cfrmf53r
p0125Corn anther prophase I*p0125.czaab26r
p0126Corn leaf tissue From V8-V10 stages, pooled, night-p0126.cnlbv55r
harvested
p0128Corn primary and secondary immature earp0128.cpiac95r
p0128.cpide86r
p0128.cpiar27r
rds2cRice developing seeds from middle of the plantrds2c.pk010.n9
r10nRice 15 day old leaf*r10n.pk0033.e3
r10n.pk0061.b10
r10n.pk111.f7
r10n.pk114.d10
r10n.pk0010.g 12
r10n.pk093.h6
rlr48Rice leaf 15 days after germination, 48 hours after infectionrlr48.pk0025.c4
of strain Magaporthe grisea 4360-R-62 (AVR2-YAMO)rls48.pk0025.d10
rlr72Rice leaf 15 days after germination, 72 hours after infectionrlr72.pk0008.e6
of strain Magaporthe grisea 4360-R-62 (AVR2-YAMO)
rls6Rice leaf 15 days after germination, 6 hours after infectionrls6.pk0005.a8
of strain Magaporthe grisea 4360-R-67 (AVR2-YAMO)rls6.pk0054.e10
rr1Rice root of two week old developing seedlingrr1.pk0049.e8
rrl1pk0051.a2
rsl1nRice 15-day-old seedling*rsl1n.pk010.m8
rtc1cRice leaf inoculated with Magaporthe griseastrain 0184rtc1c.pk005.k11.f
at 4, 8 and 24 hours
scb1cSoybean embryogenic suspension culturescb1c.pk004.g21
sdp2cSoybean developing pods (6-7 mm)sdp2c.pk003.a21
sdp3cSoybean developing pods (8-9 mm)sdp3c.pk005.c8
se2Soybean embryo, 13 days after floweringse2.pk0011.a6
se3Soybean embryo, 17 days after floweringse3.pk0036.f9
se4Soybean embryo, 19 days after floweringse4.pk0034.g5
se5Soybean embryo, 21 days after floweringse5.pk0039.b1
se5.pk0050.h4
ses4dSoybean embryogenic suspension 4 days after subcultureses4d.pk0035.h2
ses8wSoybean mature embryo 8 weeks after subcultureses8w.pk0009.g12
sfl1Soybean immature flowersfl1.pk135.m11
sfl1.pk0062.d9
sgs4cSoybean seeds 2 days after germinationsgs4c.pk004.e3
src2cSoybean 8 day old root inoculated with eggs of cystsrc2c.pk003.h10
nematode Heterodera glycines (Race 1) for 4 days.src2c.pk006.p3
src2c.pk003.17
src2c.pk001.c19
src2c.pk001.g10
src2c.pk011.f20
src2c.pk016.g24
src2c.pk021.120
src2c.pk029.g20
src3cSoybean 8 day old root infected with cyst nematodesrc3c.pk001.k23
Heterodera glycines
src3c.pk007.p21
src3c.pk010.b10
src3c.pk016.g17
src3c.pk021.i7
src3c.pk023.j17
sreSoybean root elongation zone 4 to 5 days after germinationsre.pk0017.d6
sre.pk0009.g6
srr1cSoybean control for src1c (8 day old root inoculated withsrr1c.pk001.o3
eggs of cyst nematode, Heterodera glycines (Race 1) for 4srr1c.pk001.d13
days).srr1c.pk001.a24
srr2cSoybean control for src2c (8 day old root inoculated withsrr2c.pk001.b6
eggs of cyst nematode Heterodera glycines (Race 1) for 4srr2c.pk003.j11
days)
ss1Soybean seedling 5-10 days after germinationss1.pk0002.e3
ssmSoybean shoot meristemssm.pk0059.d10
ssm.pk0028.g8
wdk1cWheat developing kernel, 3 days after anthesiswdk1c.pk0004.f8
wdk1c.pk008.d16
wdk3cWheat developing kernel, 14 days after anthesiswdk3c.pk023.i19
wle1nWheat leaf from 7 day old etiolated seedling*wle1n.pk0078.d6
wle1n.pk0091.e7
wle1n.pk0068.c6
wle1n.pk0074.a9
wlk8Wheat seedlings 8 hours after treatmentwlk8.pk0002.f4
with herbicide*****
wlm96Wheat seedlings 96 hr after inoculation w/ E. graminiswlm96.pk043.m13
wlm96.pk028.e8
wr1Wheat root from 7 day old seedlingwr1.pk0083.d6
wr1.pk0099.h10
wre1nWheat root from 7 day old etiolated seedling*wre1n.pk0102.e7
wre1n.pk0104.f8
wre1n.pk0015.d11
ytc055cRice yeast two-hybrid library constructed with, rtc1c (riceytc055c.pk001.j11.f
leaf inoculated with M. grisea strain 0184 at 4, 8 and 24
hours)
*These libraries were normalized essentially as described in U.S. Pat. No. 5,482,845, incorporated herein by reference.
**Corn developmental stages are explained in the publication “How a corn plant develops” from the Iowa State University Coop. Ext. Service Special Report No. 48 reprinted June 1993.
***Chemicals used included nitrilotriacetic acid, mercaptobenzothiazole, diethyldithiocarbamate
****Application of 2-[(2,4-dihydro-2,6,9-trimethyl[1]benzothiopyrano[4,3-c]pyrazol-8-yl)carbonyl]-1,3-cyclohexanedione S,S-dioxide; synthesis and methods of using this compound are described in WO 97/19087, incorporated herein by reference. Chemicals used in tissue treatments were: chloramphenicol, cyclohexamide, sorbitol, egosterol, taxifolin, caffeine, trigonelline, diphenylene iodonium Cl, methotrexate, BHQ,
# cyclopiazonic acid, nifedipine, verapamil, fluphenizine-N-2-chloroethane, neomycin sulfate, LY 294002, suramin, aurintricarboxylic acid, wortmannin, MAS 7, dipyridamole, zarinast, 8 bromo cGMP, a 23187, calmidazoilum chloride, compound 48/80, 1,2,Didecanoyl rac glycerol, staurosporine, trequinsin HCl, FTS, hydroxyurea, aphidicolin, tunicamycin, brefeldin A, Valinomycin, D-Mannose, Hydrogen peroxide, D-galactose, bafilomycin A1, Oligomycin, lonomycin, paraquat, glutathione,
# N acetyl-L-cysteine, nitrilotriacetic acid, mercaptobenzothiazole, diethyldithiocarbamate, aminotriazole, alpha-amino adipic acid, ancymidol, HC-toxin, okadaic acid, K-252a, A3, H-7, olomoucine, rapamycin, cyclosporin A, calyculin A, cypermethrin, actinomysin D, cytochalsain B, all of which are commercially available from Calbiochem-Novabiochem Corp.
*****Application of 6-iodo-2-propoxy-3-propyl-4(3H)-quinazolinone; synthesis and methods of using this compound are described in USSN 08/545,827, incorporated herein by reference.
******Chemicals used included chloramphenicol, cyclohexamide, aurintricarboylic acid
TABLE 3 — BLAST Results for Sequences Encoding Polypeptides Homologous to Various Plant Metabolism Proteins BLAST pLog Score (NCBI General
CloneStatusIdentifier No.)
Dioxygenase
p0128.cpiac95rEST27.30(gi 1666096)
rr1.pk0049.e8EST14.20(gi 1666096)
Contig composed of:Contig150.00(gi 1666096)
se3.pk0036.f9
src2c.pk003.h10
src2c.pk003.17
src2c.pk011.f20
src2c.pk016.g24
src2c.pk021.120
src2c.pk029.g20
src3c.pk001.k23
src3c.pk007.p21
src3c.pk010.b10
src3c.pk016.g17
src3c.pk021.i7
src3c.pk023.j17
srr1c.pk001.o3
srr2c.pk001.b6
ss1.pk0002.e3
wle1n.pk0078.d6EST7.00(gi 1666096)
Ent-Kaurene Synthase A
rl0n.pk111.f7EST13.70(gi 2642661)
sfl1.pk135.m11EST25.70(gi 2160544)
Ent-Kaurene Synthase B
Contig composed of:Contig71.70(gi 1431870)
p0014.ctutf13rb
p0083.cldec87r
p0117.chclk47ra
p0121.cfrmf53r
rsl1n.pk010.m8EST31.30(gi 3056725)
wre1n.pk0102.e7EST39.00(gi 3056725)
GA-20 Oxidase
Contig composd of:Contig172.00(gi 2222798)
cbn10.pk0055.e7
cbn10.pk0061.a4
cco1n.pk084.i6
cen3n.pk0196.g3
cen3n.pk0211.h10
cr1n.pk0018.e6
p0044.cjrai81r
p0085.cscag24r
p0119.cmtoh11r
p0128.cpide86r
rds2c.pk010.n9EST36.00(gi 2222798)
se2.pk0011.a6EST97.70(gi 1381673)
Gibberellin 3-beta
Hydroxylase
cen3n.pk0019.d1014.00(gi 3834350)
se5.pk0039.b128.00(gi 4164145)
wdk1c.pk0004.f812.20(gi 2316102)
wlm96.pk043.m1323.30(gi 3834350)
GTP cyclohydrolase II/3,4-
dihydroxy-2-butanone-4-
phosphate synthase
p0126.cnlbv55rFIS166.00(gi 2462925)
rl0n.pk0061.b10FIS141.00(gi 2462925)
wle1n.pk0091.e7FIS104.00(gi 2462925)
Riboflavin specfic deaminase
cs1.pk0038.d1FIS45.00(gi 5262786)
rls6.pk0005.a8FIS98.00(gi 5262786)
wdk1c.pk008.d16FIS117.00(gi 5541706)
Ethylene Response
Factor/Ethylene Receptor
Gene
cep7.pk0016.h9EST10.20(gi 4650821)
p0119.cmtnf84rEST8.00(gi 5733831)
Contig of:CGS>254.00(gi 2281705)
cbn10.pk0015.b5
cco1.pk0035.d12
p0031.ccmar94r
p0031.ccmar94rx
p0059.cmsba25r
p0072.comgu73rb
p0107.cbcas83r
p0110.cgsmr50r
p0110.cgsnq75r
p0110.cgsnq76r
p0125.czaab26r
p0128.cpiar27r
rl0n.pk114.d10EST55.20(gi 2281705)
scb1c.pk004.g21EST71.20(gi 4138853)
sdp2c.pk003.a21EST67.70(gi 4877653)
wdk3c.pk023.i19EST7.40(gi 4650821)
Acyl-Coa thioesterase II
Contig composed of:Contig51.70(gi 3047124)
cbn10.pk0023.h4
cpi1c.pk014.m16
rlr48.pk0025.c4FIS54.70(gi 4885565)
sgs4c.pk004.e3EST7.70(gi 1213545)
Contig composed of:Contig21.70(gi 3047124)
src2c.pk001.c19
src2c.pk006.p3
ABC transporter GCN20-like
ccase-b.pk0001.a3FIS>254.00(gi 6633814)
rr1.pk0051.a2FIS>254.00(gi 6633814)
ssm.pk0059.d10FIS80.10(gi 6633814)
wre1n.pk0104.f8FIS83.00(gi 6633814)
ABC transporter PDR5-like
cr1n.pk0147.b9FIS28.00(gi 4581139)
cs1.pk0080.b11FIS>254.00(gi 2062169)
rls48.pk0025.d10FIS>254.00(gi 2062169)
srr2c.pk003.j11FIS54.30(gi 8072390)
wr1.pk0083.d6FIS>254.00(gi 1514643)
P-glycoprotein 1
cen3n.pk0005.f9EST48.50(gi 7268557)
chpc24.pk0004.b2EST21.40(gi 4558552)
cs1.pk0051.e12FIS101.00(gi 4558552)
wle1n.pk0068.c6EST38.10(gi 8571454)
wr1.pk0099.h10FIS89.70(gi 3522943)
P-glycoprotein 3
cs1.pk0067.d8EST69.20(gi 2739309)
P-glycoprotein ATPGP
cr1n.pk0002.a6FIS12.30(gi 7268557)
cr1n.pk0195.h6FIS139.00(gi 8468012)
sfl1.pk0062.d9FIS86.70(gi 6573748)
ssm.pk0028.g8EST76.00(gi 7442648)
P-glycoprotein HVMDR2
rl0n.pk0010.g12EST53.70(gi 7486813)
srr1c.pk001.d13FIS125.00(gi 7442649)
ABC Transporter
Contig composed of:CGS>254.00(gi 4115931)
cco1.pk0025.b9
cpf1c.pk003.15
rlr72.pk0008.e6CGS>254.00(gi 4115931)
srr1c.pk001.a24CGS>254.00(gi 4115931)
wlk8.pk0002.f4CGS>254.00(gi 4115931)
PMP70 ABC Transporter
cco1.pk0004.f8FIS84.10(gi 7487703)
rl0n.pk093.h6FIS40.70(gi 4585979)
ses4d.pk0035.h2FIS54.00(gi 7487703)
wle1n.pk0074.a9FIS40.00(gi 4585979)
MRP4 ABC Transporter
cen3n.pk0045.f5FIS50.30(gi 7485868)
cr1n.pk0020.f7FIS32.40(gi 2980641)
cr1n.pk0176.b9FIS28.40(gi 7362750)
Contig composed of:Contig172.00(gi 2316016)
ccase-b.pk0009.c11
p0080.cgabm66r
rls6.pk0054.e10FIS>254.00(gi 7076769)
sdp3c.pk005.c8FIS178.00(gi 2316016)
se5.pk0050.h4FIS152.00(gi 7485868)
sre.pk0009.g6FIS>254.00(gi 2959767)
wlm96.pk028.e8FIS133.00(gi 2316016)
wre1n.pk0015.d11FIS121.00(gi 2316016)
TABLE 4 — BLAST Results for Sequences Encoding Polypeptides Homologous to Plant Metabolism Proteins BLAST pLog Score (NCBI General
CloneStatusIdentifier)
Dioxygenase
p0128.cpiac95rCGS103.00(gi 4678586)
rr1.pk0049.e8CGS104.00(gi 4678586)
src3c.pk010.b10CGS150.00(gi 1666096)
wle1n.pk0078.d6CGS105.00(gi 4678586)
Ent-Kaurene Synthase A
rl0n.pk111.f7FIS22.30(gi 4151195)
ytc055c.pk001.j11.fEST47.00(gi 7489781)
sfl1.pk135.m11CGS>254.00(gi 2160544)
Ent-Kaurene Synthase B
Contig composed of:Contig>254.00(gi 7484763)
p0014.ctutf13r
p0014.ctutf13rb
p0083.cldec87r
p0117.chclk47ra
p0121.cfrmf53r
rsl1n.pk010.m8EST31.30(gi 3056725)
wre1n.pk0102.e7EST39.00(gi 3056725)
GA-20 Oxidase
p0128.cpide86rCGS153.00(gi 743320)
rds2c.pk010.n9CGS125.00(gi 2222798)
se2.pk0011.a6FIS142.00(gi 1381673)
Gibberellin 3-beta Hydroxylase
cen3n.pk0019.d10FIS19.10(gi 5813796)
se4.pk0034.g5CGS96.00(gi 4164145)
wdk1c.pk0004.f8FIS25.50(gi 3924597)
wlm96.pk043.m13EST23.30(gi 3834350)
GTP cyclohydrolase II/3,4-
dihydroxy-2-butanone-4-
phosphate synthase
cp1c.pk004.k22CGS109.00(gi 2462925)
cta1.pk0006.g8FIS68.40(gi 1346113)
p0126.cnlbv55rCGS166.00(gi 2462925)
rl0n.pk0033.e3FIS143.00(gi 2462925)
Contig composed of:CGS168.00(gi 2462925)
rl0n.pk0061.b10
rtc1c.pk005.k11.f
sre.pk0017.d6 (CGS)CGS>254.00(gi 2462925)
wle1n.pk0091.e7FIS104.00(gi 2462925)
Riboflavin specfic deaminase
cs1.pk0038.d1FIS45.00(gi 5262786)
rls6.pk0005.a8FIS98.00(gi 5262786)
wdk1c.pk008.d16FIS117.00(gi 5541706)
Ethylene response factor
cep7.pk0016.h9EST10.20(gi 4650821)
p0119.cmtnf84rCGS>254.00(gi 6136818)
p0128.cpiar27rCGS>254.00(gi 7489538)
rl0n.pk114.d10CGS>254.00(gi 4154359)
scb1c.pk004.g21CGS>254.00(gi 4138853)
sdp2c.pk003.a21FIS93.70(gi 4877653)
wdk3c.pk023.i19CGS159.00(gi 2281705)
Acyl-CoA thioesterase
Contig composed of:Contig51.70(gi 3047124)
cbn10.pk0023.h4
cpi1c.pk014.m16
rlr48.pk0025.c4CGS54.70(gi 4885565)
sgs4c.pk004.e3EST7.70(gi 1213545)
src2c.pk001.c19CGS53.50(gi 3047124)
Contig composed of:CGS60.20(gi 3047124)
ses8w.pk0009.g12
src2c.pk001.g10
TABLE 5 — Percent Identity of Amino Acid Sequences Deduced From the Nucleotide Sequences of cDNA Clones Encoding Polypeptides Homologous to Plant Metabolism Proteins Percent Identity to
SEQ ID NO.(NCBI General Identifier)
Dioxygenase
247% (gi 4678586)
438% (gi 4678586)
670% (gi 1666096)
840% (gi 4678586)
Ent-Kaurene Synthase A
1860% (gi 4151195)
2055% (gi 7489781)
2271% (gi 2160544)
Ent-Kaurene Synthase B
2441% (gi 7484763)
2654% (gi 3056725)
2843% (gi 3056725)
GA-20 Oxidase
3650% (gi 7433220)
3856% (gi 2222798)
4077% (gi 1381673)
Gibberellin 3-beta Hydroxylase
4232% (gi 5813796)
4449% (gi 4164145)
4629% (gi 3924597)
4846% (gi 3834350)
GTP cyclohydrolase II/3,4-
dihydroxy-2-butanone-4-phosphate.
synthase
6267% (gi 2462925)
6466% (gi 1346113)
6655% (gi 2462925)
6879% (gi 2462925)
7059% (gi 2462925)
7271% (gi 2462925)
7468% (gi 2462925)
Riboflavin specific deaminase
7657% (gi 5262786)
7879% (gi 5262786)
8026% (gi 5262786)
Ethylene response factor
8443% (gi 4650821)
8649% (gi 6136818)
8892% (gi 7489538)
9069% (gi 4154359)
9297% (gi 4138853)
9474% (gi 4877653)
9676% (gi 2281705)
Acyl-CoA thioesterase
11045% (gi 3047124)
11235% (gi 4885565)
11432% (gi 1213545)
11648% (gi 3047124)
11855% (gi 3047124)
ABC transporters GCN20-like
12878% (gi 6633814)
13080 (gi 6633814)
13292% (gi 6633814)
13485% (gi 6633814)
ABC transporters PDR5-like
13636% (gi 4581139)
13863% (gi 2062169)
14070% (gi 2062169)
14260% (gi 8072390)
14473% (gi 1514643)
P-glycoprotein 1
14672% (gi 7268557)
14862% (gi 4558552)
15051% (gi 4558552)
15271% (gi 8571454)
15474% (gi 3522943)
P-glycoprotein 3
15670% (gi 2739309)
P-glycoprotein ATPGP
15837% (gi 7268557)
16070% (gi 8468012)
16264% (gi 6573748)
16461% (gi 7442648)
P-glycoprotein HVMDR2
16686% (gi 7486813)
16851% (gi 7442649)
ABC Transporter
17066% (gi 4115931)
17267% (gi 4115931)
17477% (gi 4115931)
17668% (gi 4115931)
PMP70 ABC Transporter
17850% (gi 7487703)
18041% (gi 4585979)
18246% (gi 7487703)
18455% (gi 4585979)
MRP4 ABC Transporter
18687% (gi 7485868)
18860% (gi 2980641)
19070% (gi 7362750)
19258% (gi 2316016)
19443% (gi 7076769)
19672% (gi 2316016)
19884% (gi 7485868)
20070% (gi 2959767)
20273% (gi 2316016)
20459% (gi 2316016)
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19 codes
IPC · International Patent Classification
Section A — Human necessities
  • A01H3/00
Section C — Chemistry; metallurgy
  • C12N9/00
  • C07H21/04
  • C12N5/14
  • C07K14/415
USPC · US Patent Classification
800/278530/370536/23.2435/410435/69.1435/252.3530/350800/295435/419435/6536/23.6536/24.1435/320.1435/183

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