Schizochytrium PKS genes

This application is a continuation-in-part of U.S. Ser. No. 09/090,793, filed Jun. 4, 1998, which is a continuation-in-part of provisional application U.S. Ser. No. 60/048,650, filed Jun. 4, 1997, which is incorporated herein by reference.

1. Field of the Invention

This invention relates to modulating levels of enzymes and/or enzyme components capable of modifying long chain poly-unsaturated fatty acids (PUFAs) in a host cell, and constructs and methods for producing PUFAs in a host cell. The invention is exemplified by production of eicosapentenoic acid (EPA) using genes derived from Shewanella putrefaciens and Vibrio marinus.

2. Background

Two main families of poly-unsaturated fatty acids (PUFAs) are the ω3 fatty acids, exemplified by eicosapentenoic acid, and the ω6 fatty acids, exemplified by arachidonic acid. PUFAs are important components of the plasma membrane of the cell, where they can be found in such forms as phospholipids, and also can be found in triglycerides. PUFAs also serve as precursors to other molecules of importance in human beings and animals, including the prostacyclins, leukotrienes and prostaglandins. Long chain PUFAs of importance include docosahexenoic acid (DHA) and eicosapentenoic acid (EPA), which are found primarily in different types of fish oil, gamma-linolenic acid (GLA), which is found in the seeds of a number of plants, including evening primrose ( Oenothera biennis ), borage ( Borago officinalis ) and black currants ( Ribes nigrum ), stearidonic acid (SDA), which is found in marine oils and plant seeds, and arachidonic acid (ARA), which along with GLA is found in filamentous fungi. ARA can be purified from animal tissues including liver and adrenal gland. Several genera of marine bacteria are known which synthesize either EPA or DHA. DHA is present in human milk along with ARA.

PUFAs are necessary for proper development, particularly in the developing infant brain, and for tissue formation and repair. As an example, DHA, is an important constituent of many human cell membranes, in particular nervous cells (gray matter), muscle cells, and spermatozoa and believed to affect the development of brain functions in general and to be essential for the development of eyesight. EPA and DHA have a number of nutritional and pharmacological uses. As an example adults affected by diabetes (especially non insulin-dependent) show deficiencies and imbalances in their levels of DHA which are believed to contribute to later coronary conditions. Therefore a diet balanced in DHA may be beneficial to diabetics.

For DHA, a number of sources exist for commercial production including a variety of marine organisms, oils obtained from cold water marine fish, and egg yolk fractions. The purification of DHA from fish sources is relatively expensive due to technical difficulties, making DHA expensive and in short supply. In algae such as Amphidinium and Schizochytrium and marine fungi such as Thraustochytrium DHA may represent up to 48% of the fatty acid content of the cell. A few bacteria also are reported to produce DHA. These are generally deep sea bacteria such as Vibrio marinus . For ARA, microorganisms including the genera Mortierella, Entomophthora, Phytium and Porphyridium can be used for commercial production. Commercial sources of SDA include the genera Trichodesma and Echium. Commercial sources of GLA include evening primrose, black currants and borage. However, there are several disadvantages associated with commercial production of PUFAs from natural sources. Natural sources of PUFA, such as animals and plants, tend to have highly heterogeneous oil compositions. The oils obtained from these sources can require extensive purification to separate out one or more desired PUFA or to produce an oil which is enriched in one or more desired PUFA.

Natural sources also are subject to uncontrollable fluctuations in availability. Fish stocks may undergo natural variation or may be depleted by overfishing. Animal oils, and particularly fish oils, can accumulate environmental pollutants. Weather and disease can cause fluctuation in yields from both fish and plant sources. Cropland available for production of alternate oil-producing crops is subject to competition from the steady expansion of human populations and the associated increased need for food production on the remaining arable land. Crops which do produce PUFAs, such as borage, have not been adapted to commercial growth and may not perform well in monoculture. Growth of such crops is thus not economically competitive where more profitable and better established crops can be grown. Large -scale fermentation of organisms such as Shewanella also is expensive. Natural animal tissues contain low amounts of ARA and are difficult to process. Microorganisms such as Porphyridium and Shewanella are difficult to cultivate on a commercial scale.

Dietary supplements and pharmaceutical formulations containing PUFAs can retain the disadvantages of the PUFA source. Supplements such as fish oil capsules can contain low levels of the particular desired component and thus require large dosages. High dosages result in ingestion of high levels of undesired components, including contaminants. Care must be taken in providing fatty acid supplements, as overaddition may result in suppression of endogenous biosynthetic pathways and lead to competition with other necessary fatty acids in various lipid fractions in vivo, leading to undesirable results. For example, Eskimos having a diet high in ω3 fatty acids have an increased tendency to bleed (U.S. Pat. No. 4,874,603). Fish oils have unpleasant tastes and odors, which may be impossible to economically separate from the desired product, such as a food supplements. Unpleasant tastes and odors of the supplements can make such regimens involving the supplement undesirable and may inhibit compliance by the patient.

A number of enzymes have been identified as being involved in PUFA biosynthesis. Linoleic acid (LA, 18:2 Δ9, 12) is produced from oleic acid (18:1 Δ9) by a Δ12-desaturase. GLA (18:3 Δ9, 12) is produced from linoleic acid (LA, 18:2 Δ9, 12) by a A6-desaturase. ARA (20:4 Δ5, 8, 11, 14) is produced from DGLA (20:3 Δ8, 11, 14), catalyzed by a Δ5-desaturase. Eicosapentenoic acid (EPA) is a 20 carbon, omega 3 fatty acid containing 5 double bonds (Δ5, 8, 11, 14, 17), all in the cis configuration. EPA, and the related DHA (Δ4, 7, 10, 13, 16, 19, C22:6) are produced from oleic acid by a series of elongation and desaturation reactions. Additionally, an elongase (or elongases) is required to extend the 18 carbon PUFAs out to 20 and 22 carbon chain lengths. However, animals cannot convert oleic acid (18:1 Δ9) into linoleic acid (18:2 Δ9, 12). Likewise, μ-linolenic acid (ALA, 18:3 Δ9, 12, 15) cannot be synthesized by mammals. Other eukaryotes, including fungi and plants, have enzymes which desaturate at positions Δ12 and Δ15. The major poly-unsaturated fatty acids of animals therefore are either derived from diet and/or from desaturation and elongation of linoleic acid (18:2 Δ9, 12) or μ-linolenic acid (18:3 Δ9, 12, 15).

Poly-unsaturated fatty acids are considered to be useful for nutritional, pharmaceutical, industrial, and other purposes. An expansive supply of poly-unsaturated fatty acids from natural sources and from chemical synthesis are not sufficient for commercial needs. Because a number of separate desaturase and elongase enzymes are required for fatty acid synthesis from linoleic acid (LA, 18:2 Δ9, 12), common in most plant species, to the more saturated and longer chain PUFAs, engineering plant host cells for the expression of EPA and DHA may require expression of five or six separate enzyme activities to achieve expression, at least for EPA and DHA, and for production of quantities of such PUFAs additional engineering efforts may be required, for instance the down regulation of enzymes competing for substrate, engineering of higher enzyme activities such as by mutagenesis or targeting of enzymes to plastid organelles. Therefore it is of interest to obtain genetic material involved in PUFA biosynthesis from species that naturally produce these fatty acids and to express the isolated material alone or in combination in a heterologous system which can be manipulated to allow production of commercial quantities of PUFAs.

Relevant Literature

Several genera of marine bacteria have been identified which synthesize either EPA or DHA (DeLong and Vayanos, Applied and Environmental Microbiology (1986) 51: 730-737). Researchers of the Sagami Chemical Research Institute have reported EPA production in E. coli which have been transformed with a gene cluster from the marine bacterium, Shewanella putrefaciens . A minimum of 5 open reading frames (ORFs) are required for fatty acid synthesis of EPA in E. coli .To date, extensive characterization of the functions of the proteins encoded by these genes has not been reported (Yazawa (1996) Lipids 31, S-297; WO 93/23545; WO 96/21735).

The protein sequence of open reading frame (ORF) 3 as published by Yazawa, U.S. Pat. No. 5,683,898 is not a functional protein. Yazawa defines the protein as initiating at the methionine codon at nucleotides 9016-9014 of the Shewanella PKS-like cluster (Genbank accession U73935) and ending at the stop codon at nucleotides 8185-8183 of the Shewanella PKS-like cluster. However, when this ORF is expressed under control of a heterologous promoter in an E. Coli strain containing the entire PKS-like cluster except ORF 3, the recombinant cells do not produce EPA.

Polyketides are secondary metabolites the synthesis of which involves a set of enzymatic reactions analogous to those of fatty acid synthesis (see reviews: Hopwood and Shernan, Annu. Rev. Genet . (1990) 24: 37-66, and Katz and Donadio, in Annual Review of Microbiology (1993) 47: 875-912). It has been proposed to use polyketide synthases to produce novel antibiotics (Hutchinson and Fujii, Annual Review of Microbiology (1995) 49:201-238).

Novel compositions and methods are provided for preparation of long chain poly-unsaturated fatty acids (PUFAs) using polyketide-like synthesis (PKS-like) genes in plants and plant cells. In contrast to the known and proposed methods for production of PUFAs by means of fatty acid synthesis genes, by the invention constructs and methods are provided for producing PUFAs by utilizing genes of a PKS-like system. The methods involve growing a host cell of interest transformed with an expression cassette functional in the host cell, the expression cassette comprising a transcriptional and translational initiation regulatory region, joined in reading frame 5′ to a DNA sequence to a gene or component of a PKS-like system capable of modulating the production of PUFAs (PKS-like gene). An alteration in the PUFA profile of host cells is achieved by expression following introduction of a complete PKS-like system responsible for a PUFA biosynthesis into host cells. The invention finds use for example in the large scale production of DHA and EPA and for modification of the fatty acid profile of host cells and edible plant tissues and/or plant parts.

FIG. 1 provides designations for the ORFs of the EPA gene cluster of Shewanella. FIG. 1A shows the organization of the genes; those ORFs essential for EPA production in E. coli are numbered. FIG. 1B shows the designations given to subclones.

FIG. 2 provides the Shewanella PKS-like domain structure, motifs and ‘Blast’ matches of ORF 6 (FIG. 2 A), ORF 7 (FIG. 2 B), ORF 8 (FIG. 2 C), ORF 9 (FIG. 2D) and ORF 3 (FIG. 2 E). FIG. 2F shows the structure of the region of the Anabeana chromosome that is related to domains present in Shewanella EPA ORFs.

FIG. 3 shows results for pantethenylation—ORF 3 in E. coli strain SJ16. The image shows [C 14 ] β-Alanine labelled proteins from E. coli (strain SJ16) cells transformed with the listed plasmids. Lane 1 represents pUC19, lane 2 represents pPA-NEB (ΔORF 3), lane 3 represents pAA-Neb (EPA+), lane 4 represents ORF 6 subclone, lane 5 represents ORF 6+ORF 3 subclones, and lane 6 represents ORF 3 subclone. ACP and an unknown (but previously observed) 35 kD protein were labelled in all of the samples. The high molecular mass proteins detected in lanes 2 and 5 are full-length (largest band) and truncated products of the Shewanella ORF-6 gene (confirmed by Western analysis). E. Coli strain SJ16 is conditionally blocked in β-alanine synthesis.

FIG. 4A shows the DNA sequence (SEQ ID NO:1) for the PKS-like cluster found in Shewanella, containing ORF's 3-9. FIG. 4B shows the amino acid sequence (SEQ ID NO:2) of ORF 2, which is coded by nucleotides 6121-8103 of the sequence shown in FIG. 4 A. FIG. 4C shows the amino acid sequence (SEQ ID NO:3) of the published, inactive ORF3, translated from the strand complementary to that shown in FIG. 4A, nucleotides 9016-8186. FIG. 4D shows the nucleotide sequence 8186-9157 (SEQ ID NO:4); its complementary strand codes for ORF 3 active in EPA synthesis. FIGS. 4E-J show the amino acid sequences (SEQ ID NOS:5-10) corresponding to ORF's 4-9, which are encoded by nucleotides 9681-12590 (SEQ ID NO:81), 13040-13903 (SEQ ID NO:82), 13906-22173 (SEQ ID NO:83), 22203-24515 (SEQ ID NO:84), 24518-30529 (SEQ ID NO:85) and 30730-32358 (SEQ ID NO:86), respectively, of FIG. 4 A. FIG. 4K shows the amino acid sequence (SEQ ID NO:11) corresponding to nucleotides 32834-34327.

FIG. 5 shows the sequence (SEQ ID NO:12) for the PKS-like cluster in an approximately 40 kb DNA fragment of Vibrio marinus , containing ORFs 6, 7, 8 and 9. The start and last codons for each ORF are as follows: ORF 6: 17394, 25352; ORF 7: 25509, 28160; ORF 8: 28209, 34265; ORF 9: 34454, 36118.

FIG. 6 shows the sequence (SEQ ID NO:13) for an approximately 19 kb portion of the PKS-like cluster of FIG. 5 which contains the ORFs 6, 7, 8 and 9. The start and last codons for each ORF are as follows: ORF 6: 411, 8369 (SEQ ID NO:77); ORF 7: 8526, 11177 (SEQ ID NO:78); ORF 8: 11226, 17282 (SEQ ID NO:79); ORF 9: 17471, 19135 (SEQ ID NO:80).

FIG. 7 shows a comparison of the PKS-like gene clusters of Shewanella putrefaciens and Vibrio marinus ; FIG. 7B is the Vibrio marinus operon sequence.

FIG. 8 is an expanded view of the PKS-like gene cluster portion of Vibrio marinus shown in FIG. 7B showing that ORFs 6, 7 and 8 are in reading frame 2, while ORF 9 is in reading frame 3.

FIG. 9 demonstrates sequence homology of ORF 6 of Shewanella putrefaciens and Vibrio marinus . The Shewanella ORF 6 is depicted on the vertical axis, and the Vibrio ORF 6 is depicted on the horizontal axis. Lines indicate regions of the proteins that have a 60% identity. The repeated lines in the middle correspond to the multiple ACP domains found in ORF 6.

FIG. 10 demonstrates sequence homology of ORF 7 of Shewanella putrefaciens and Vibrio marinus . The Shewanella ORF 7 is depicted on the vertical axis, and the Vibrio ORF 7 is depicted on the horizontal axis. Lines indicate regions of the proteins that have a 60% identity.

FIG. 11 demonstrates sequence homology of ORF 8 of Shewanella putrefaciens and Vibrio marinus . The Shewanella ORF 8 is depicted on the vertical axis, and the Vibro. ORF 8 is depicted on the horizontal axis. Lines indicate regions of the proteins that have a 60% identity.

FIG. 12 demonstrates sequence homology of ORF 9 of Shewanella putrefaciens and Vibrio marinus . The Shewanella ORF 9 is depicted on the vertical axis, and the Vibrio ORF 9 is depicted on the horizontal axis. Lines indicate regions of the proteins that have a 60% identity.

FIG. 13 is a depiction of various complementation experiments, and resulting PUFA production. On the right, is shown the longest PUFA made in the E. coli strain containing the Vibrio and Shewanella genes depicted on the left. The hollow boxes indicate ORFs from Shewanella. The solid boxes indicate ORFs from Vibrio.

FIG. 14 is a chromatogram showing fatty acid production from complementation of pEPAD8 from Shewanella (deletion ORF 8) with ORF 8 from Shewanella, in E. coli Fad E-. The chromatogram presents an EPA (20:5) peak.

FIG. 15 is a chromatogram showing fatty acid production from complementation of pEPAD8 from Shewanella (deletion ORF 8) with ORF 8 from Vibrio marinus , in E. coil Fad E-. The chromatograph presents EPA (20:5) and DHA (22:6) peaks.

FIG. 16 is a table of PUFA values from the ORF 8 complementation experiment, the chromatogram of which is shown in FIG. 15 .

FIG. 17 is a plasmid map showing the elements of pCGN7770.

FIG. 18 is a plasmid map showing the elements of pCGN8535.

FIG. 19 is a plasmid map showing the elements of pCGN8537.

FIG. 20 is a plasmid map showing the elements of pCGN8525.

FIG. 21 is a comparison of the Shewanella ORFs as defined by Yazawa (1996) supra, and those disclosed in FIG. 4 . When a protein starting at the leucine (TTG) codon at nucleotides 9157-9155 and ending at the stop codon at nucleotides 8185-8183 is expressed under control of a heterologous promoter in an E. coli strain containing the entire PKS-like cluster except ORF 3, the recombinant cells do produce EPA. Thus, the published protein sequence is likely to be wrong, and the coding sequence for the protein may start at the TTG codon at nucleotides 9157-9155 or the TTG codon at nucleotides 9172-9170. This information is critical to the expression of a functional PKS-like cluster heterologous system.

FIG. 22 is a plasmid map showing the elements of pCGN8560.

FIG. 23 is plasmid map showing the elements of pCGN8556.

FIG. 24 shows the translated DNA sequence (SEQ ID NO:14) upstream of the published ORF 3 and the corresponding amino acids for which they code (SEQ ID NO:15). The ATG start codon at position 9016 is the start codon for the protein described by Yazawa et al (1996) supra. The other arrows depict TTG or ATT codons that can also serve as start codons in bacteria. When ORF 3 is started from the published ATG codon at 9016, the protein is not functional in making EPA. When ORF 3 is initiated at the TTG codon at position 9157, the protein is capable of facilitating EPA synthesis.

FIG. 25 shows the PCR product (SEQ ID NO:16) for SS9 Photobacter using primers in Example 1.

FIG. 26 shows probe sequences (SEQ ID NOS:17-31) resulting from PCR with primers presented in Example 1.

FIG. 27 shows the nucleotide sequence of Schizochytrium EST clones A. LIB3033-047-B5, LIB3033-046-E6 and a bridging PCR product have now been assembled into a partial cDNA sequence (ORF6 homolog), B. LIB3033-046-D2 (hglc/ORF7/ORF8/ORF9 homolog), C. LIB81-015-D5, LIB81-042-B9 and a bridging PCR product have now been assembled into a partial cDNA sequence (ORF8/ORF9 homolog).

FIG. 28 shows a schematic of the similarities between Shewanella PKS sequences and Schizochytrium sequences.

FIG. 29 shows the amino acid sequences inferred from Schizochytrium EST clones A. ORF6 homolog, B. hglc/ORF7/ORF8/ORF9 homolog, C. ORF8/0RF9 homolog.

In accordance with the subject invention, novel DNA sequences, DNA constructs and methods are provided, which include some or all of the polyketide-like synthesis (PKS-like) pathway genes from Shewanella, Vibrio, Schizochytrium or other microorganisms, for modifying the poly-unsaturated long chain fatty acid content of host cells, particularly host plant cells. The present invention demonstrates that EPA synthesis genes in Shewanella putrefaciens constitute a polyketide-like synthesis pathway. Functions are ascribed to the Shewanella, Schizochytrium and Vibrio genes and methods are provided for the production of EPA and DHA in host cells. The method includes the step of transforming cells with an expression cassette comprising a DNA encoding a polypeptide capable of increasing the amount of one or more PUFA in the host cell. Desirably, integration constructs are prepared which provide for integration of the expression cassette into the genome of a host cell. Host cells are manipulated to express a sense or antisense DNA encoding a polypeptide(s) that has PKS-like gene activity. By “PKS-like gene” is intended a polypeptide which is responsible for any one or more of the functions of a PKS-like activity of interest. By “polypeptide” is meant any chain of amino acids, regardless of length or post-translational modification, for example, glycosylation or phosphorylation. Depending upon the nature of the host cell, the substrate(s) for the expressed enzyme may be produced by the host cell or may be exogenously supplied. Of particular interest is the selective control of PUFA production in plant tissues and/or plant parts such as leaves, roots, fruits and seeds. The invention can be used to synthesize EPA, DHA, and other related PUFAs in host cells.

There are many advantages to transgenic production of PUFAs. As an example, in transgenic E. coli as in Shewanella, EPA accumulates in the phospholipid fraction, specifically in the sn-2 position. It may be possible to produce a structured lipid in a desired host cell which differs substantially from that produced in either Shewanella or E. coli . Additionally transgenic production of PUFAs in particular host cells offers several advantages over purification from natural sources such as fish or plants. In transgenic plants, by utilizing a PKS-like system, fatty acid synthesis of PUFAs is achieved in the cytoplasm by a system which produces the PUFAs through de novo production of the fatty acids utilizing malonyl Co-A and acetyl Co-A as substrates. In this fashion, potential problems, such as those associated with substrate competition and diversion of normal products of fatty acid synthesis in a host to PUFA production, are avoided.

Production of fatty acids from recombinant plants provides the ability to alter the naturally occurring plant fatty acid profile by providing new synthetic pathways in the host or by suppressing undesired pathways, thereby increasing levels of desired PUFAs, or conjugated forms thereof, and decreasing levels of undesired PUFAs. Production of fatty acids in transgenic plants also offers the advantage that expression of PKS-like genes in particular tissues and/or plant parts means that greatly increased levels of desired PUFAs in those tissues and/or parts can be achieved, making recovery from those tissues more economical. Expression in a plant tissue and/or plant part presents certain efficiencies, particularly where the tissue or part is one which is easily harvested, such as seed, leaves, fruits, flowers, roots, etc. For example, the desired PUFAs can be expressed in seed; methods of isolating seed oils are well established. In addition to providing a source for purification of desired PUFAs, seed oil components can be manipulated through expression of PKS-like genes, either alone or in combination with other genes such as elongases, to provide seed oils having a particular PUFA profile in concentrated form. The concentrated seed oils then can be added to animal milks and/or synthetic or semisynthetic milks to serve as infant formulas where human nursing is impossible or undesired, or in cases of malnourishment or disease in both adults and infants.

Transgenic microbial production of fatty acids offers the advantages that many microbes are known with greatly simplified oil compositions as compared with those of higher organisms, making purification of desired components easier. Microbial production is not subject to fluctuations caused by external variables such as weather and food supply. Microbially produced oil is substantially free of contamination by environmental pollutants. Additionally, microbes can provide PUFAs in particular forms which may have specific uses. For example, Spirulina can provide PUFAs predominantly at the first and third positions of triglycerides; digestion by pancreatic lipases preferentially releases fatty acids from these positions. Following human or animal ingestion of triglycerides derived from Spirulina, these PUFAs are released by pancreatic lipases as free fatty acids and thus are directly available, for example, for infant brain development. Additionally, microbial oil production can be manipulated by controlling culture conditions, notably by providing particular substrates for microbially expressed enzymes, or by addition of compounds which suppress undesired biochemical pathways. In addition to these advantages, production of fatty acids from recombinant microbes provides the ability to alter the naturally occurring microbial fatty acid profile by providing new synthetic pathways in the host or by suppressing undesired pathways, thereby increasing levels of desired PUFAs, or conjugated forms thereof, and decreasing levels of undesired PUFAs.

Production of fatty acids in animals also presents several advantages. Expression of desaturase genes in animals can produce greatly increased levels of desired PUFAs in animal tissues, making recovery from those tissues more economical. For example, where the desired PUFAs are expressed in the breast milk of animals, methods of isolating PUFAs from animal milk are well established. In addition to providing a source for purification of desired PUFAs, animal breast milk can be manipulated through expression of desaturase genes, either alone or in combination with other human genes, to provide animal milks with a PUFA composition substantially similar to human breast milk during the different stages of infant development. Humanized animal milks could serve as infant formulas where human nursing is impossible or undesired, or in the cases of malnourishment or disease.

DNAs encoding desired PKS-like genes can be identified in a variety of ways. In one method, a source of a desired PKS-like gene, for example genomic libraries from a Shewanella, Schizochytrium or Vibrio spp., is screened with detectable enzymatically- or chemically-synthesized probes. Sources of ORFs having PKS-like genes are those organisms which produce a desired PUFA, including DHA-producing or EPA-producing deep sea bacteria growing preferentially under high pressure or at relatively low temperature. Microorgansims such as Shewanella which produce EPA or DHA also can be used as a source of PKS-like genes. The probes can be made from DNA, RNA, or non-naturally occurring nucleotides, or mixtures thereof. Probes can be enzymatically synthesized from DNAs of known PKS-like genes for normal or reduced-stringency hybridization methods. For discussions of nucleic acid probe design and annealing conditions, see, for example, Sambrook et al, Molecular Cloning: A Laboratory Manual (2 nd ed.), Vols. 1-3 , Cold Spring Harbor Laboratory , (1989) or Current Protocols in Molecular Biology , F. Ausubel et al, ed., Greene Publishing and Wiley-Interscience, New York (1987), each of which is incorporated herein by reference. Techniques for manipulation of nucleic acids encoding PUFA enzymes such as subcloning nucleic acid sequences encoding polypeptides into expression vectors, labelling probes, DNA hybridization, and the like are described generally in Sambrook, supra.

Oligonucleotide probes also can be used to screen sources and can be based on sequences of known PKS-like genes, including sequences conserved among known PKS-like genes, or on peptide sequences obtained from a desired purified protein. Oligonucleotide probes based on amino acid sequences can be degenerate to encompass the degeneracy of the genetic code, or can be biased in favor of the preferred codons of the source organism. Alternatively, a desired protein can be entirely sequenced and total synthesis of a DNA encoding that polypeptide performed.

Once the desired DNA has been isolated, it can be sequenced by known methods. It is recognized in the art that such methods are subject to errors, such that multiple sequencing of the same region is routine and is still expected to lead to measurable rates of mistakes in the resulting deduced sequence, particularly in regions having repeated domains, extensive secondary structure, or unusual base compositions, such as regions with high GC base content. When discrepancies arise, resequencing can be done and can employ special methods. Special methods can include altering sequencing conditions by using: different temperatures; different enzymes; proteins which alter the ability of oligonucleotides to form higher order structures; altered nucleotides such as ITP or methylated dGTP; different gel compositions, for example adding formamide; different primers or primers located at different distances from the problem region; or different templates such as single stranded DNAs. Sequencing of mRNA can also be employed.

For the most part, some or all of the coding sequences for the polypeptides having PKS-like gene activity are from a natural source. In some situations, however, it is desirable to modify all or a portion of the codons, for example, to enhance expression, by employing host preferred codons. Host preferred codons can be determined from the codons of highest frequency in the proteins expressed in the largest amount in a particular host species of interest. Thus, the coding sequence for a polypeptide having PKS-like gene activity can be synthesized in whole or in part. All or portions of the DNA also can be synthesized to remove any destabilizing sequences or regions of secondary structure which would be present in the transcribed mRNA. All or portions of the DNA also can be synthesized to alter the base composition to one more preferable to the desired host cell. Methods for synthesizing sequences and bringing sequences together are well established in the literature. In vitro mutagenesis and selection, site-directed mutagenesis, or other means can be employed to obtain mutations of naturally occurring PKS-like genes to produce a polypeptide having PKS-like gene activity in vivo with more desirable physical and kinetic parameters for function in the host cell, such as a longer half-life or a higher rate of production of a desired polyunsaturated fatty acid.

Of particular interest are the Shewanella putrefaciens ORFs and the corresponding ORFs of Vibrio marinus and Schizochytrium. The Shewanella putrefaciens PKS-like genes can be expressed in transgenic plants to effect biosynthesis of EPA. Other DNAs which are substantially identical in sequence to the Shewanella putrefaciens PKS-like genes, or which encode polypeptides which are substantially similar to PKS-like genes of Shewanella putrefaciens can be used, such as those identified from Vibrio marinus or Schizochytrium. By substantially identical in sequence is intended an amino acid sequence or nucleic acid sequence exhibiting in order of increasing preference at least 60%, 80%, 90% or 95% homology to the DNA sequence of the Shewanella putrefaciens PKS-like genes or nucleic acid sequences encoding the amino acid sequences for such genes. For polypeptides, the length of comparison sequences generally is at least 16 amino acids, preferably at least 20 amino acids, and most preferably 35 amino acids. For nucleic acids, the length of comparison sequences generally is at least 50 nucleotides, preferably at least 60 nucleotides, and more preferably at least 75 nucleotides, and most 20 preferably, 110 nucleotides.

Homology typically is measured using sequence analysis software, for example, the Sequence Analysis software package of the Genetics Computer Group, University of Wisconsin Biotechnology Center, 1710 University Avenue, Madison, Wis. 53705, MEGAlign (DNAStar, Inc., 1228 S. Park St., Madison, Wis. 53715), and MacVector (Oxford Molecular Group, 2105 S. Bascom Avenue, Suite 200, Campbell, Calif. 95008). BLAST (National Center for Biotechnology Information (WCBI) www.ncbi.nlm.gov; FASTA:(Pearson and Lipman, Science (1985) 227:1435-1446). Such software matches similar sequences by assigning degrees of homology to various substitutions, deletions, and other modifications. Conservative substitutions typically include substitutions within the following groups: glycine and alanine; valine, isoleucine and leucine; aspartic acid, glutamic acid, asparagine, and glutamine; serine and threonine; lysine and arginine; and phenylalanine and tyrosine. Substitutions may also be made on the basis of conserved hydrophobicity or hydrophilicity (Kyte and Doolittle, J. Mol. Biol . (1982) 157: 105-132), or on the basis of the ability to assume similar polypeptide secondary structure (Chou and Fasman, Adv. Enzymol . (1978) 47: 45-148, 1978). A related protein to the probing sequence is identified when p≧0.01, preferably p≧10 −7 or 10 −8 .

Encompassed by the present invention are related PKS-like genes from the same or other organisms. Such related PKS-like genes include variants of the disclosed PKS-like ORFs that occur naturally within the same or different species of Shewanella, as well as homologues of the disclosed PKS-like genes from other species and evolutionarily related proteins having analogous function and activity. Also included are PKS-like genes which, although not substantially identical to the Shewanella putrefaciens PKS-like genes, operate in a similar fashion to produce PUFAs as part of a PKS-like system. Related PKS-like genes can be identified by their ability to function substantially the same as the disclosed PKS-like genes; that is, they can be substituted for corresponding ORFs of Shewanella, Schizochytrium or Vibrio and still effectively produce EPA or DHA. Related PKS-like genes also can be identified by screening sequence databases for sequences homologous to the disclosed PKS-like genes, by hybridization of a probe based on the disclosed PKS-like genes to a library constructed from the source organism, or by RT-PCR using mRNA from the source organism and primers based on the disclosed PKS-like gene. Thus, the phrase “PKS-like genes” refers not only to the nucleotide sequences disclosed herein, but also to other nucleic acids that are allelic or species variants of these nucleotide sequences. It is also understood that these terms include nonnatural mutations introduced by deliberate mutation using recombinant technology such as single site mutation or by excising short sections of DNA open reading frames coding for PUFA enzymes or by substituting new codons or adding new codons. Such minor alterations substantially maintain the immunoidentity of the original expression product and/or its biological activity. The biological properties of the altered PUFA enzymes can be determined by expressing the enzymes in an appropriate cell line and by determining the ability of the enzymes to synthesize PUFAs. Particular enzyme modifications considered minor would include substitution of amino acids of similar chemical properties, e.g., glutamic acid for aspartic acid or glutamine for asparagine.

When utilizing a PUFA PKS-like system from another organism, the regions of a PKS-like gene polypeptide important for PKS-like gene activity can be determined through routine mutagenesis, expression of the resulting mutant polypeptides and determination of their activities. The coding region for the mutants can include deletions, insertions and point mutations, or combinations thereof. A typical functional analysis begins with deletion mutagenesis to determine the N- and C-terminal limits of the protein necessary for function, and then internal deletions, insertions or point mutants are made in the open ready frame to further determine regions necessary for function. Other techniques such as cassette mutagenesis or total synthesis also can be used. Deletion mutagenesis is accomplished, for example, by using exonucleases to sequentially remove the 5′ or 3′ coding regions. Kits are available for such techniques. After deletion, the coding region is completed by ligating oligonucleotides containing start or stop codons to the deleted coding region after 5′ or 3′ deletion, respectively. Alternatively, oligonucleotides encoding start or stop codons are inserted into the coding region by a variety of methods including site-directed mutagenesis, mutagenic PCR or by ligation onto DNA digested at existing restriction sites. Internal deletions can similarly be made through a variety of methods including the use of existing restriction sites in the DNA, by use of mutagenic primers via site directed mutagenesis or mutagenic PCR. Insertions are made through methods such. as linker-scanning mutagenesis, site-directed mutagenesis or mutagenic PCR. Point mutations are made through techniques such as site-directed mutagenesis or mutagenic PCR.

Chemical mutagenesis also can be used for identifying regions of a PKS-like gene polypeptide important for activity. A mutated construct is expressed, and the ability of the resulting altered protein to function as a PKS-like gene is assayed. Such structure-function analysis can determn e which regions may be deleted, which regions tolerate insertions, and which point mutations allow the mutant protein to function in substantially the same way as the native PKS-like gene. All such mutant proteins and nucleotide sequences encoding them are within the scope of the present invention. EPA is produced in Shewanella as the product of a PKS-like system, such that the EPA genes encode components of this system. In Vibrio, DHA is produced by a similar system. The enzymes which synthesize these fatty acids are encoded by a cluster of genes which are distinct from the fatty acid synthesis genes encoding the enzymes involved in synthesis of the C16 and C18 fatty acids typically found in bacteria and in plants. As the Shewanella EPA genes represent a PKS-like gene cluster, EPA production is, at least to some extent, independent of the typical bacterial type II FAS system. Thus, production of EPA in the cytoplasm of plant cells can be achieved by expression of the PKS-like pathway genes in plant cells under the control of appropriate plant regulatory signals.

EPA production in E. coli transformed with the Shewanella EPA genes proceeds during anaerobic growth, indicating that O 2 -dependent desaturase reactions are not involved. Analyses of the proteins encoded by the ORFs essential for EPA production reveals the presence of domain structures characteristic of PKS-like systems. FIG. 2A shows a summary of the domains, motifs, and also key homologies detected by “BLAST” data bank searches. Because EPA is different from many of the other substances produced by PKS-like pathways, i.e., it contains 5, cis double bonds, spaced at 3 carbon intervals along the molecule, a PKS-like system for synthesis of EPA is not expected.

Further, BLAST searches using the domains present in the Shewanella EPA ORFs reveal that several are related to proteins encoded by a PKS-like gene cluster found in Anabeana. The structure of that region of the Anabeana chromosome is shown in FIG. 2 F. The Anabeana PKS-like genes have been linked to the synthesis of a long-chain (C26), hydroxy-fatty acid found in a glycolipid layer of heterocysts. The EPA protein domains with homology to the Anabeana proteins are indicated in FIG. 2 F.

ORF 6 of Shewanella contains a KAS domain which includes an active site motif (DXAC*), SEQ ID NO:32, as well as a “GFGG”, SEQ ID NO:33, motif which is present at the end of many Type II KAS proteins (see FIG. 2 A). Extended motifs are present but not shown here. Next is a malonyl-CoA:ACP acyl transferase (AT) domain. Sequences near the active site motif (GHS*XG), SEQ ID NO:34, suggest it transfers malonate rather than methylmalonate, i.e., it resembles the acetate-like ATs. Following a linker region, there is a cluster of 6 repeating domains, each ˜100 amino acids in length, which are homologous to PKS-like ACP sequences. Each contains a pantetheine binding site motif 2S (LGXDS*(L/I)), SEQ ID NOS:35 and 36. The presence of 6 such ACP domains has not been observed previously in fatty acid synthases (FAS) or PKS-like systems. Near the end of the protein is a region which shows homology to β-keto-ACP reductases (KR). It contains a pyridine nucleotide binding site motif “GXGXX(G/A/P)”, SEQ ID NOS:37, 38 and 39.

The Shewanella ORF 8 begins with a KAS domain, including active site and ending motifs (FIG. 2 C). The best match in the data banks is with the Anabeana HglD. There is also a domain which has sequence homology to the N-terminal one half of the Anabeana HglC. This region also shows weak homology to KAS proteins although it lacks the active site and ending motifs. It has the characteristics of the so-called chain length factors (CLF) of Type II PKS-like systems. ORF 8 appears to direct the production of EPA versus DHA by the PKS-like system. ORF 8 also has two domains with homology to β-hydroxyacyl-ACP dehydrases (DH). The best match for both domains is with E. coli FabA, a bi-functional enzyme which carries out both the dehydrase reaction and an isomerization (trans to cis) of the resulting double bond. The first DH domain contains both the active site histidine (H) and an adjacent cysteine (C) implicated in FabA catalysis. The second DH domain has the active site H but lacks the adjacent C (FIG. 2 C). Blast searches with the second DH domain also show matches to FabZ, a second E. coli DH, which does not possess isomerase activity.

The N-terminal half of ORF 7 (FIG. 2B) has no significant matches in the data banks. The best match of the C-terminal half is with a C-terminal portion of the Anabeana HglC. This domain contains an acyl-transferase (AT) motif (GXSXG), SEQ ID NO:40. Comparison of the extended active site sequences, based on the crystal structure of the E. coli malonyl-CoA:ACP AT, reveals that ORF 7 lacks two residues essential for exclusion of water from the active site ( E. coli nomenclature; Q11 and R117). These data suggest that ORF 7 may function as a thioesterase.

ORF 9 (FIG. 2D) is homologous to an ORF of unknown function in the Anabeana Hgl cluster. It also exhibits a very weak homology to NIFA, a regulatory protein in nitrogen fixing bacteria. A regulatory role for the ORF 9 protein has not been excluded. ORF 3 (FIG. 2E) is homologous to the Anabeana HetI as well as EntD from E. coli and Sfp of Bacillus. Recently, a new enzyme family of phosphopantetheinyl transferases has been identified that includes HetI, EntD and Sfp (Lamblot RH, et al. (1996) A new enzyme superfamily—the phophopantetheinyl transferases. Chemistry & Biology , Vol 3, #11, 923-936 ). The data of FIG. 3 demonstrates that the presence of ORF 3 is required for addition of β-alanine (i.e. pantetheine) to the ORF 6 protein. Thus, ORF 3 encodes the phosphopantetheinyl transferase specific for the ORF 6 ACP domains. (See, Haydock SF et al. (1995) Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-CoA:acyl carrier protein transacylase domains in modular polyketide synthases, FEBS Lett ., 374, 246-248). Malonate is the source of the carbons utilized in the extension reactions of EPA synthesis. Additionally, malonyl-CoA rather than malonyl-ACP is the AT substrate, i.e., the AT region of ORF 6 uses malonyl Co-A.

Once the DNA sequences encoding the PKS-like genes of an organism responsible for PUFA production have been obtained, they are placed in a vector capable of replication in a host cell, or propagated in vitro by means of techniques such as PCR or long PCR. Replicating vectors can include plasmids, phage, viruses, cosmids and the like. Desirable vectors include those useful for mutagenesis of the gene of interest or for expression of the gene of interest in host cells. A PUFA synthesis enzyme or a homologous protein can be expressed in a variety of recombinantly engineered cells. Numerous expression systems are available for expression of DNA encoding a PUFA enzyme. The expression of natural or synthetic nucleic acids encoding PUFA enzyme is typically achieved by opera bly linking the DNA to a promoter (which is either constitutive or inducible) within an expression vector. By expression vector is meant a DNA molecule, linear or circular, that comprises a segment encoding a PUFA enzyme, operably linked to additional segments that provide for its transcription. Such additional segments include promoter and terminator sequences. An expression vector also may include one or more origins of replication, one or more selectable markers, an enhancer, a polyadenylation signal, etc. Expression vectors generally are derived from plasmid or viral DNA, and can contain elements of both. The term “operably linked” indicates that the segments are arranged so that they function in concert for their intended purposes, for example, transcription initiates in the promoter and proceeds through the coding segment to the terminator. See Sambrook et al, supra.

The technique of long PCR has made in vitro propagation of large constructs possible, so that modifications to the gene of interest, such as mutagenesis or addition of expression signals, and propagation of the resulting constructs can occur entirely in vitro without the use of a replicating vector or a host cell. In vitro expression can be accomplished, for example, by placing the coding region for the desaturase polypeptide in an expression vector designed for in vitro use and adding rabbit reticulocyte lysate and cofactors; labeled amino acids can be incorporated if desired. Such in vitro expression vectors may provide some or all of the expression signals necessary in the system used. These methods are well known in the art and the components of the system are commercially available. The: reaction mixture can then be assayed directly for PKS-like enzymes for example by determining their activity, or the synthesized enzyme can be purified and then assayed.

Expression in a host cell can be accomplished in a transient or stable fashion. Transient expression can occur from introduced constructs which contain expression signals functional in the host cell, but which constructs do not replicate and rarely integrate in the host cell, or:where the host cell is not proliferating. Transient expression also can be accomplished by inducing the activity of a regulatable promoter operably linked to the gene of interest, although such inducible systems frequently exhibit a low basal level of expression. Stable expression can be achieved by introduction of a nucleic acid construct that can integrate into the host genome or that autonomously replicates in the host cell. Stable expression of the gene of interest can be selected for through the use of a selectable marker located on or transfected with the expression construct, followed by selection for cells expressing the marker. When stable expression results from integration, integration of constructs can occur randomly within the host genome or can be targeted through the use of constructs containing regions of homology with the host genome sufficient to target recombination with the host locus. Where constructs are targeted to an endogenous locus, all or some of the transcriptional and translational regulatory regions can be provided by the endogenous locus. To achieve expression in a host cell, the transformed DNA is operably associated with transcriptional and translational initiation and termination regulatory regions that are functional in the host cell.

Transcriptional and translational initiation and termination regions are derived from a variety of nonexclusive sources, including the DNA to be expressed, genes known or suspected to be capable of expression in the desired system, expression vectors, chemical synthesis The termination region can be derived from the 3′ region of the gene from which the initiation region was obtained or from a different gene. A large number of termination regions are known to and have been found to be satisfactory in a variety of hosts from the same and different genera and species. The termination region usually is selected more as a matter of convenience rather than because of any particular property. When expressing more than one PKS-like ORF in the same cell, appropriate regulatory regions and expression methods should be used. Introduced genes can be propagated in the host cell through use of replicating vectors or by integration into the host genome. Where two or more genes are expressed from separate replicating vectors, it is desirable that each vector has a different means of replication. Each introduced construct, whether integrated or not, should have a different means of selection and should lack homology to the other constructs to maintain stable expression and prevent reassortment of elements among constructs. Judicious choices of regulatory regions, selection means and method of propagation of the introduced construct can be experimentally determined so that all introduced genes are expressed at the necessary levels to provide for synthesis of the desired products.

A variety of procaryotic expression systems can be used to express PUFA enzyme. Expression vectors: can be constructed which contain a promoter to direct transcription, a ribosome binding site, and a transcriptional terminator. Examples of regulatory regions suitable for this purpose in E. coli are the promoter and operator region of the E. coli tryptophan biosynthetic pathway as described by Yanofsky (1984) J. Bacteriol ., 158:1018-1024 and the leftward promoter of phage lambda (Pλ) as described by Herskowitz and Hagen, (1980) Ann. Rev. Genet ., 14:399-445. The inclusion of selection markers in DNA vectors transformed in E. coli is also useful. Examples of such markers include genes specifying resistance to ampicillin, tetracycline, or chloramphenicol. Vectors used for expressing foreign genes in bacterial hosts generally will contain a selectable marker, such as a gene for antibiotic resistance, and a promoter which functions in the host cell. Plasmids useful for transforming bacteria include pBR322 (Bolivar, et al, (1977) Gene 2:95-113), the pUC plasmids (Messing.(1983) Meth. Enzymol . 101:20-77, Vieira and Messing, (1982) Gene 19:259-268), pCQV2 (Queen, ibid.), and derivatives thereof. Plasmids may contain both viral and bacterial elements. Methods for the recovery of the proteins in biologically active form are discussed in U.S. Pat. Nos. 4,966,963 and 4,999,422, which are incorporated herein by reference. See Sambrook, et al for a description of other prokaryotic expression systems.

For expression in eukaryotes, host cells for use in practicing the present invention include mammalian, avian, plant, insect, and fungal cells. As an example, for plants, the choice of a promoter will depend in part upon whether constitutive or inducible expression is desired and whether it is desirable to produce the PUFAs at a particular stage of plant development and/or in a particular tissue. Considerations for choosing a specific tissue and/or developmental stage for expression of the ORFs may depend on competing substrates or the ability of the host cell to tolerate expression of a particular PUFA. Expression can be targeted to a particular location within a host plant such as seed, leaves, fruits, flowers, and roots, by using specific regulatory sequences, such as those described in U.S. Pat. No. 5,463,174, U.S. Pat. No. 4,943,674, U.S. Pat. No. 5,106,739, U.S. Pat. No. 5,175,095, U.S. Pat. No. 5,420,034, U.S. Pat. No. 5,188,958, and U.S. Pat. No. 5,589,379. Where the host cell is a yeast, transcription and translational regions functional in yeast cells are provided, particularly from the host species. The transcriptional initiation regulatory regions can be obtained, for example from genes in the glycolytic pathway, such as alcohol dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase (GPD), phosphoglucoisomerase, phosphoglycerate kinase, etc. or regulatable genes such as acid phosphatase, lactase, metallothionein, glucoamylase, etc. Any one of a number of regulatory sequences can be used in a particular situation, depending upon whether constitutive or induced transcription is desired, the particular efficiency of the promoter in conjunction with the open-reading frame of interest, the ability to join a strong promoter with a control region from a different promoter which allows for inducible transcription, ease of construction, and the like. Of particular interest are promoters which are activated in the presence of galactose. Galactose-inducible promoters (GAL1, GAL7, and GAL10) have been extensively utilized for high level and regulated expression of protein in yeast (Lue et al, (1987) Mol. Cell. Biol . 7:3446; Johnston, (1987) Microbiol. Rev . 51:458). Transcription from the GAL promoters is activated by the GAL4 protein, which binds to the promoter region and activates transcription when galactose is present. In the absence of galactose, the antagonist GAL80 binds to GAL4 and prevents GAL4 from activating transcription. Addition of galactose prevents GAL80 from inhibiting activation by GAL4. Preferably, the termination region is derived from a yeast gene, particularly Saccharomyces, Schizosaccharomyces, Candida or Kluyveromyces. The 3′ regions of two mammalian genes, γ interferon and α2 interferon, are also known to function in yeast.

Nucleotide sequences surrounding the translational initiation codon ATG have been found to affect expression in yeast cells. If the desired polypeptide is poorly expressed in yeast, the nucleotide sequences of exogenous genes can be modified to include an efficient yeast translation initiation sequence to obtain optimal gene expression. For expression in Saccharomyces, this can be done by site-directed mutagenesis of an inefficiently expressed gene by fusing it in-frame to an endogenous Saccharomyces gene, preferably a highly expressed gene, such as the lactase gene.

As an alternative to expressing the PKS-like genes in the plant cell cytoplasm, is to target the enzymes to the chloroplast. One method to target proteins to the chloroplast entails use of leader peptides attached to the N-terrnini of the proteins. Commonly used leader peptides are derived from the small subunit of plant ribulose bis phosphate carboxylase. Leader sequences from other chloroplast proteins may also be used. Another method for targeting proteins to the chloroplast is to transform the chloroplast genome (Stable transformation of chloroplasts of Chlamydomonas reinhardtii (1 green alga) using bombardment of recipient cells with high-velocity tungsten microprojectiles coated with foreign DNA has been described. See, for example, Blowers et al Plant Cell (1989) 1:123-132 and Debuchy et al EMBO J (1989) 8:2803-2809. The transformation technique, using tungsten microprojectiles, is described by Kline et al, Nature (London) (1987) 327:70-73). The most common method of transforming chloroplasts involves using biolistic techniques, but other techniques developed for the purpose may also be used. (Methods for targeting foreign gene products into chloroplasts (Shrier et al EMBO J . (1985) 4:25-32) or mitochnodria (Boutry et al, supra) have been described. See also Tomai et al Gen. Biol. Chem . (1988) 263:15104-15109 and U.S. Pat. No. 4,940,835 for the use of transit peptides for translocating nuclear gene products into the chloroplast. Methods for directing the transport of proteins to the chloroplast are reviewed in Kenauf TIBTECH (1987) 5:40-47.

For producing PUFAs in avian species and cells, gene transfer can be performed by introducing a nucleic acid sequence encoding a PUFA enzyme into the cells following procedures known in the art. If a transgenic animal is desired, pluripotent stem cells of embryos can be provided with a vector carrying a PUFA enzyme encoding transgene and developed into adult animal (U.S. Pat. No. 5,162,215; Ono et al. (1996) Comparative Biochemistry and Physiolog A 113(3):287-292; WO 9612793; WO 9606160). In most cases, the transgene is modified to express high levels of the PKS-like enzymes in order to increase production of PUFAs. The transgenes can be modified, for example, by providing transcriptional and/or translational regulatory regions that function in avian cells, such as promoters which direct expression in particular tissues and egg parts such as yolk. The gene regulatory regions can be obtained from a variety of sources, including chicken anemia or avian leukosis viruses or avian genes such as a chicken ovalbumin gene.

Production of PUFAs in insect cells can be conducted using baculovirus expression vectors harboring PKS-like transgenes. Baculovirus expression vectors are available from several commercial sources such as Clonetech. Methods for producing hybrid and transgenic strains of algae, such as marine algae, which contain and express a desaturase transgene also are provided. For example, transgenic marine algae can be prepared as described in U.S. Pat. No. 5,426,040. As with the other expression systems described above, the timing, extent of expression and activity of the desaturase transgene can be regulated by fitting the polypeptide coding sequence with the appropriate transcriptional and translational regulatory regions selected for a particular use. Of particular interest are promoter regions which can be induced under preselected growth conditions. For example, introduction of temperature sensitive and/or metabolite responsive mutations into the desaturase transgene coding sequences, its regulatory regions, and/or the genome of cells into which the transgene is introduced can be used for this purpose.

The transformed host cell is grown under appropriate conditions adapted for a desired end result. For host cells grown in culture, the conditions are typically optimized to produce the greatest or most economical yield of PUFAs, which relates to the selected desaturase activity. Media conditions which may be optimized include: carbon source, nitrogen source, addition of substrate, final concentration of added substrate, form of substrate added, aerobic or anaerobic growth, growth temperature, inducing agent, induction temperature, growth phase at induction, growth phase at harvest, pH, density, and maintenance of selection. Microorganisms such as yeast, for example, are preferably grown using selected media of interest, which include yeast peptone broth (YPD) and minimal media (contains amino acids, yeast nitrogen base, and ammonium sulfate, and lacks a component for selection, for example uracil). Desirably, substrates to be added are first dissolved in ethanol. Where necessary, expression of the polypeptide of interest may be induced, for example by including or adding galactose to induce expression from a GAL promoter.

When increased expression of the PKS-like gene polypeptide in a host cell which expresses PUFA from a PKS-like system is desired, several methods can be employed. Additional genes encoding the PKS-like gene polypeptide can be introduced into the host organism. Expression from the native PKS-like gene locus also can be increased through homologous recombination, for example by inserting a stronger promoter into the host genome to cause increased expression, by removing destabilizing sequences from either the mRNA or the encoded protein by deleting that information from the host genome, or by adding stabilizing sequences to the mRNA (see U.S. Pat. No. 4,910,141 and U.S. Pat. No. 5,500,365). Thus, the subject host will have at least have one copy of the expression construct and may have two or more, depending upon whether the gene is integrated into the genome, amplified, or is present on an extrachromosomal element having multiple copy numbers. Where the subject host is a yeast, four principal types of yeast plasmid vectors can be used: Yeast Integrating plasmids (YIps), Yeast Replicating plasmids (YRps), Yeast Centromere plasmids (YCps), and Yeast Episomal plasmids (YEps). YIps lack a yeast replication origin and must be propagated as integrated elements in the yeast genome. YRps have a chromosomally derived autonomously replicating sequence and are propagated as medium copy number (20 to 40), autonomously replicating, unstably segregating plasmids. YCps have both a replication origin and a centromere sequence and propagate as low copy number (10-20), autonomously replicating, stably segregating plasmids. YEps have an origin of replication from the yeast 2 μm plasmid and are propagated as high copy number, autonomously replicating, irregularly segregating plasmids. The presence of the plasmids in yeast can be ensured by maintaining selection for a marker on the plasmid. Of particular interest are the yeast vectors pYES2 (a YEp plasmid available from Invitrogen, confers uracil prototrophy and a GAL1 galactose-inducible promoter for expression), and pYX424 (a YEp plasmid having a constitutive TP1 promoter and conferring leucine prototrophy; (Alber and Kawasaki (1982). J. Mol. & Appl. Genetics 1:419).

The choice of a host cell is influenced in part by the desired PUFA profile of the transgenic cell, and the native profile of the host cell. Even where the host cell expresses PKS-like gene activity for o ne PUFA, expression of PKS-like genes of another PKS-like system can provide for production of a novel PUFA not produced by the host cell. In particular instances where expression of PKS-like gene activity is coupled with expression of an ORF 8 PKS-like gene of an organism which produces a different PUFA, it can be desirable that the host cell naturally have, or be mutated to have, low PKS-like gene activity for ORF 8. As an example, for production of EPA, the DNA sequence used encodes the polypeptide having PKS-like gene activity of an organism which produces EPA, while for production of DHA, the DNA sequences used are those from an organism which produces DHA. For use in a host cell which already expresses PKS-like gene activity it can be necessary to utilize an expression cassette which provides for overexpression of the desired PKS-like genes alone or with a construct to downregulate the activity of an existing ORF of the existing PKS-like system, such as by antisense or co-suppression. Similarly, a; combination of ORFs derived from separate organisms which produce the same or different PUFAs using PKS-like systems may be used. For instance, the ORF 8 of Vibrio directs the expression of DHA in a host cell, even when ORFs 3, 6, 7 and 9 are from Shewanella, which produce EPA when coupled to ORF 8 of Shewanella. Therefore, for: production of eicosapentanoic acid (EPA), the expression cassettes used generally include one or more cassettes which include ORFs 3, 6, 7, 8 and 9 from a PUFA-producing organism such as the marine bacterium Shewanella putrefaciens (for EPA production) or Vibrio marinus (for DHA production). ORF 8 can be used for induction of DHA production, and ORF 8 of Vibrio can be used in conjunction with ORFs 3, 6, 7 and 9 of Shewanella to produce DHA. The organization and numbering scheme of the ORFs identified in the Shewanella gene cluster are shown in FIG. 1 A. Maps of several subclones referred to in this study are shown in FIG. 1 B. For expression of a PKS-like gene polypeptide, transcriptional and translational initiation and termination regions functional in the host cell are operably linked to the DNA encoding the PKS-like gene polypeptide.

Constructs comprising the PKS-like ORFs of interest can be introduced into a host cell by any of a variety of standard techniques, depending in part upon the type of host cell. These techniques include transfection, infection, bolistic impact, electroporation, microinjection, scraping, or any other method which introduces the gene of interest into the host cell (see U.S. Pat. No. 4,743,548, U.S. Pat. No. 4,795,855, U.S. Pat. No. 5,068,193, U.S. Pat. No. 5,188,958, U.S. Pat. No. 5,463,174, U.S. Pat. No. 5,565,346 and U.S. Pat. No. 5,565,347). Methods of transformation which are used include lithium acetate transformation ( Methods in Enzymology , (1991) 194:186-187). For convenience, a host cell which has been manipulated by any method to take up a DNA sequence or construct will be referred to as “transformed” or “recombinant” herein. The subject host will have at least have one copy of the expression construct and may have two or more, depending upon whether the gene is integrated into the genome, amplified, or is present on an extrachromosomal element having multiple copy numbers.

For production of PUFAs, depending upon the host cell, the several polypeptides produced by pEPA, ORFs 3, 6, 7, 8 and 9, are introduced as individual expression constructs or can be combined into two or more cassettes which are introduced individually or co-transformed into a host cell. A standard transformation protocol is used. For plants, where less than all PKS-like genes required for PUFA synthesis have been inserted into a single plant, plants containing a complementing gene or genes can be crossed to obtain plants containing a full complement of PKS-like genes to synthesize a desired PUFA.

The PKS-like-mediated production of PUFAs can be performed in either prokaryotic or eukaryotic host cells. The cells can be cultured or formed as part or all of a host organism including an animal. Viruses and bacteriophage also can be used with appropriate cells in the production of PUFAs, particularly for gene transfer, cellular targeting and selection. Any type of plant cell can be used for host cells, including dicotyledonous plants, monocotyledonous plants, and cereals. Of particular interest are crop plants such as Brassica, Arabidopsis, soybean, corn, and the like. Prokaryotic cells of interest include Eschericia, Baccillus, Lactobaccillus, cyanobacteria and the like. Eukaryotic cells include plant cells, mammalian cells such as those of lactating animals, avian cells such as of chickens, and other cells amenable to genetic manipulation including insect, fungal, and algae cells. Examples of host animals include mice, rats, rabbits, chickens, quail, turkeys, cattle, sheep, pigs, goats, yaks, etc., which are amenable to genetic manipulation and cloning for rapid expansion of a transgene expressing population. For animals, PKS-like transgenes can be adapted for expression in target organelles, tissues and body fluids through modification of the gene regulatory regions. Of particular interest is the production of PUFAs in the breast milk of the host animal.

Examples of host microorganisms include Saccharomyces cerevisiae, Saccharomyces carlsbergensis , or other yeast such as Candida, Kluyveromyces or other fungi, for example, filamentous fungi such as Aspergillus, Neurospora, Penicillium, etc. Desirable characteristics of a host microorganism are, for example, that it is genetically well characterized, can be used for high level expression of the product using ultra-high density fermentation, and is on the GRAS (generally recognized as safe) list since the proposed end product is intended for ingestion by humans. Of particular interest is use of a yeast, more particularly baker's yeast ( S. cerevisiae ), as a cell host in the subject invention. Strains of particular interest are SC334 (Mat αpep4-3 prbl-1122 ura3-52 leu2-3, 112 regl-501 gal1; (Hovland et al (1989) Gene 83:57-64); BJ1995 (Yeast Genetic Stock Centre, 1021 Donner Laboratory, Berkeley, Calif. 94720), INVSC1 (Mat αhiw3Δ1 leu2 trp1-289 ura3-52 (Invitrogen, 1600 Faraday Ave., Carlsbad, Calif. 92008) and INVSC2 (Mat αhis3Δ200 ura3-167; (Invitrogen). Bacterial cells also may be used as hosts. This includes E. coli , which can be useful in fermentation processes. Alternatively, a host such as a Lactobacillus species can be used as a host for introducing the products of the PKS-like pathway into a product such as yogurt.

The transformed host cell can be identified by selection for a marker contained on the introduced construct. Alternatively, a separate marker construct can be introduced with the desired construct, as many transformation techniques introduce multiple DNA molecules into host cells. Typically, transformed hosts are selected for their ability to grow on selective media. Selective media can incorporate an antibiotic or lack a factor necessary for growth of the untransformed host, such as a nutrient or growth factor. An introduced marker gene therefor may confer antibiotic resistance, or encode an essential growth factor or enzyme, and permit growth on selective media when expressed in the transformed host cell. Desirably, resistance to kanamycin and the amino glycoside G418 are of particular interest (see U.S. Pat. No. 5,034,322). For yeast transformants, any marker that functions in yeast can be used, such as the ability to grow on media lacking uracil, lencine, lysine or tryptophan.

Selection of a transformed host also can occur when the expressed marker protein can be detected, either directly or indirectly. The marker protein can be expressed alone or as a fusion to another protein. The marker protein can be one which is detected by its enzymatic activity; for example β-galactosidase can convert the substrate X-gal to a colored product, and luciferase can convert luciferin to a light-emitting product. The marker protein can be one which is detected by its light-producing or modifying characteristics; for example, the green fluorescent protein of Aequorea Victoria fluoresces when illuminated with blue light. Antibodies can be used to detect the marker protein or a molecular tag on, for example, a protein of interest. Cells expressing the marker protein or tag can be selected, for example, visually, or by techniques such as FACS or panning using antibodies.

The PUFAs produced using the subject methods and compositions are found in the host plant tissue and/or plant part as free fatty acids and/or in conjugated forms such as acylglycerols, phospholipids, sulfolipids or glycolipids, and can be extracted from the host cell through a variety of means well-known in the art. Such means include extraction with organic solvents, sonication, supercritical fluid extraction using for example carbon dioxide, and physical means such as presses, or combinations thereof. Of particular interest is extraction with methanol and chloroform. Where appropriate, the aqueous layer can be acidified to protonate negatively charged moieties and thereby increase partitioning of desired products into the organic layer. After extraction, the organic solvents can be removed by evaporation under a stream of nitrogen. When isolated in conjugated forms, the products are enzymatically or chemically cleaved to release the free fatty acid or a less complex conjugate of interest, and are then subjected to further manipulations to produce a desired end product. Desirably, conjugated forms of fatty acids are cleaved with potassium hydroxide.

If further purification is necessary, standard methods can be employed. Such methods include extraction, treatment with urea, fractional crystallization, HPLC, fractional distillation, silica gel chromatography, high speed centrifugation or distillation, or combinations of these techniques. Protection of reactive groups, such as the acid or alkenyl groups, can be done at any step through known techniques, for example alkylation or iodination. Methods used include methylation of the fatty acids to produce methyl esters. Similarly, protecting groups can be removed at any step. Desirably, purification of fractions containing DHA and EPA is accomplished by treatment with urea and/or fractional distillation.

The uses of the subject invention are several. Probes based on the DNAs of the present invention find use in methods for isolating related molecules or in methods to detect organisms expressing PKS-like genes. When used as probes, the DNAs or oligonucleotides need to be detectable. This is usually accomplished by attaching a label either at an internal site, for example via incorporation of a modified residue, or at the 5′ or 3′ terminus. Such labels can be directly detectable, can bind to a secondary molecule that is detectably labeled, or can bind to an unlabelled secondary molecule and a detectably labeled tertiary molecule; this process can be extended as long as is practicable to achieve a satisfactorily detectable signal without unacceptable levels of background signal. Secondary, tertiary, or bridging systems can include use of antibodies directed against any other molecule, including labels or other antibodies, or can involve any molecules which bind to each other, for example a biotin-streptavidin/avidin system. Detectable labels typically include radioactive isotopes, molecules which chemically or enzymatically produce or alter light, enzymes which produce detectable reaction products, magnetic molecules, fluorescent molecules or molecules whose fluorescence or light-emitting characteristics change upon binding. Examples of labelling methods can be found in U.S. Pat. No. 5,011,770. Alternatively, the binding of target molecules can be directly detected by measuring the change in heat of solution on binding of a probe to a target via isothermal titration calorimetry, or by coating the probe or target on a surface and detecting the change in scattering of light from the surface produced by binding of a target or a probe, respectively, is done with the BlAcore system.

PUFAs produced by recombinant means find applications in a wide variety of areas. Supplementation of humans or animals with PUFAs in various forms can result in increased levels not only of the added PUFAs, but of their metabolic progeny as well.

Complex regulatory mechanisms can make it desirable to combine various PUFAs, or to add different conjugates of PUFAS, in order to prevent, control or overcome such mechanisms to achieve the desired levels of specific PUFAs in an individual. In the present case, expression of PKS-like gene genes, or antisense PKS-like gene transcripts, can alter the levels of specific PUFAs, or derivatives thereof, found in plant parts and/or plant tissues. The PKS-like gene polypeptide coding region is expressed either by itself or with other genes, in order to produce tissues and/or plant parts containing higher proportions of desired PUFAs or containing a PUFA composition which more closely resembles that of human breast milk (Prieto et al., PCT publication WO 95/24494) than does the unmodified tissues; and/or plant parts.

PUFAs, or derivatives thereof, made by the disclosed method can be used as dietary supplements for patients undergoing intravenous feeding or for preventing or treating malnutrition. For dietary supplementation, the purified PUFAs, or derivatives thereof, can be incorporated into cooking oils, fats or margarines formulated so that in normal use the recipient receives a desired amount of PUFA. The PUFAs also can be incorporated into infant formulas, nutritional supplements or other food products, and find use as anti-inflammatory or cholesterol lowering agents.

Particular fatty acids: such as EPA can be used to alter the composition of infant formulas to better replicate the PUFA composition of human breast milk. The predominant triglyceride in human milk is reported to be 1,3-di-oleoyl-2-palmitoyl, with 2-palmitoyl glycerides reported as better absorbed than 2-oleoyl or 2-lineoyl glycerides (see U.S. Pat. No. 4,876,107). Typically, human breast milk has a fatty acid profile comprising from about 0.15% to about 0.36% as DHA, from about 0.03% to about 0.13% as EPA, from about 0.30% to about 0.88% as ARA, from about 0.22% to about 0.67% as DGLA, and from about 0.27% to about 1.04% as GLA. A preferred ratio of GLA:DGLA:ARA in infant formulas is from about 1:1:4 to about 1:1:1, respectively. Amounts of oils providing these ratios of PUFA can be determined without undue experimentation by one of skill in the art. PUFAs, or host cells containing them, also can be used as animal food supplements to alter an animal's tissue or milk fatty acid composition to one more desirable for human or animal consumption.

For pharmaceutical use (human or veterinary), the compositions generally are administered orally but can be administered by any route by which they may be successfully absorbed, e.g., parenterally (i.e. subcutaneously, intramuscularly or intravenously), rectally or vaginally or topically, for example, as a skin ointment or lotion. Where available, gelatin capsules are the preferred form of oral administration. Dietary supplementation as set forth above also can provide an oral route of administration. The unsaturated acids of the present invention can be administered in conjugated forms, or as salts, esters, amides or prodrugs of the fatty acids. Any pharmaceutically acceptable salt is encompassed by the present invention; especially preferred are the sodium, potassium or lithium salts. Also encompassed are the N-alkylpolyhydroxamine salts, such as N-methyl glucamine, described in PCT publication WO 96/33155. Preferred esters are the ethyl esters.

The PUFAs of the present invention can be administered alone or in combination with a pharmaceutically acceptable carrier or excipient. As solid salts, the PUFAs can also be administered in tablet form. For intravenous administration, the PUFAs or derivatives thereof can be incorporated into commercial formulations such as Intralipids. Where desired, the individual components of formulations can be individually provided in kit form, for single or multiple use. A typical dosage of a particular fatty acid is from 0.1 mg to 20 g, or even 100 g daily, and is preferably from 10 mg to 1, 2, 5 or 10 g daily as required, or molar equivalent amounts of derivative forms thereof. Parenteral nutrition compositions comprising from about 2 to about 30 weight percent fatty acids calculated as triglycerides are encompassed by the present invention. Other vitamins, and particularly fat-soluble vitamins such as vitamin A, D, E and L-camitine optionally can be included. Where desired, a preservative such as a tocopherol can be added, typically at about 0.1% by weight.

The following examples are presented by way of illustration, not of limitation.

Shewanella putrefaciens

1

gatctcttac aaagaaacta tctcaatgtg aatttaacct taattccgtt taattacggc 60

ctgatagagc atcacccaat cagccataaa actgtaaagt gggtactcaa aggtggctgg 120

gcgattcttc tcaaatacaa agtgcccaac ccaagcaaat ccatatccga taacaggtaa 180

aagtagcaat aaaccccagc gctgagttag taatacataa gcgaataata ggatcactaa 240

actactgccg aaatagtgta atattcgaca gtttctatgc tgatgttgag ataaataaaa 300

agggtaaaat tcagcaaaag aacgatagcg cttactcatt actcacacct cggtaaaaaa 360

gcaactcgcc attaacttgg ccaatcgtca gttgttctat cgtctcaaag ttatgccgac 420

taaataactc tatatgtgca ttatgattag caaaaactcc gataccatca agatgaagtt 480

gttcatcaca ccaactcaaa actgcgtcga taagcttact gccatagccc ttgccttgct 540

ccacatttgc gatagcaata aactgtaaaa tgccacattg gccacttggt aagctctcta 600

taatctgatt ttctttgtta ataagtgcct gagttgaata ccaaccagta cttaacaaca 660

tctttaaacg ccaatgccaa aaacgcgctt cacctaaggg aacctgctga gtcactatgc 720

aggctacgcc tatcaatcta tccccaacga acataccaat aagtgcttgc tcctgttgcc 780

agagctcatt gagttcttct cgaatagccc cgcgaagctt ttgctcatac tgcgcttgat 840

caccactaaa aagtgtttcg ataaaaaagg gatcatcatg ataggcgtta tagagaatag 900

aggctgctat gcgtaaatct tctgccgtga gataaactgc acgacactct tccatggctt 960

gatcttccat tgttattgtc cttgaccttg atcacacaac accaatgtaa caagactgta 1020

tagaagtgca attaataatc aattcgtgca ttaagcaggt cagcatttct ttgctaaaca 1080

agctttattg gctttgacaa aactttgcct agactttaac gatagaaatc ataatgaaag 1140

agaaaagcta caacctagag gggaataatc aaacaactgc taagatctag ataatgtaat 1200

aaacaccgag tttatcgacc atacttagat agagtcatag caacgagaat agttatggat 1260

acaacgccgc aagatctatc acacctgttt ttacagctag gattagcaaa tgatcaaccc 1320

gcaattgaac agtttatcaa tgaccatcaa ttagcggaca atatattgct acatcaagca 1380

agcttttgga gcccatcgca aaagcacttc ttaattgagt catttaatga agatgcccag 1440

tggaccgaag tcatcgacca cttagacacc ttattaagaa aaaactaacc attacaacag 1500

caactttaaa ttttgccgta agccatctcc ccccacccca caacagcgtt gttgcttatg 1560

accactggag tacattcgtc tttagtcgtt ttaccatcac catgggtacg ttgagtgcga 1620

taaaaaagca cataaacttc tttatcggcc tgaatatagg cttcgttaaa atcagctgtt 1680

cccattaaag taaccacttg ctctttactc atgcctagag atatctttgt caaattgtca 1740

cggtttttat cttgagtttt ctcccaagca ccgtgattat cccagtcaga ttccccatca 1800

ccaacattga ccacacagcc cgttagccct aagcttgcaa tcccaaaaca tgctaaacct 1860

aataatttat ttttcatttt aacttcctgt tatgacatta tttttgctta gaagaaaagc 1920

aacttacatg ccaaaacaca agctgttgtt ttaaatgact ttatttatta ttagcctttt 1980

aggatatgcc tagagcaata ataattacca atgtttaagg aatttgacta actatgagtc 2040

cgattgagca agtgctaaca gctgctaaaa aaatcaatga acaaggtaga gaaccaacat 2100

tagcattgat taaaaccaaa cttggtaata gcatcccaat gcgcgagtta atccaaggtt 2160

tgcaacagtt taagtctatg agtgcagaag aaagacaagc aatacctagc agcttagcaa 2220

cagcaaaaga aactcaatat ggtcaatcaa gcttatctca atctgaacaa gctgatagga 2280

tcctccagct agaaaacgcc ctcaatgaat taagaaacga atttaatggg ctaaaaagtc 2340

aatttgataa cttacaacaa aacctgatga ataaagagcc tgacaccaaa tgcatgtaat 2400

tgaactacga tttgaatgtt ttgataacac cacgattact gcagcagaaa aagccattaa 2460

tggtttgctt gaagcttatc gagccaatgg ccaggttcta ggtcgtgaat ttgccgttgc 2520

atttaacgat ggtgagttta aagcacgcat gttaacccca gaaaaaagca gcttatctaa 2580

acgctttaat agtccttggg taaatagtgc actcgaagag ctaaccgaag ccaaattgct 2640

tgcgccacgt gaaaagtata ttggccaaga tattaattct gaagcatcta gccaagacac 2700

accaagttgg cagctacttt acacaagtta tgtgcacatg tgctcaccac taagaaatgg 2760

cgacaccttg cagcctattc cactgtatca aattccagca actgccaacg gcgatcataa 2820

acgaatgatc cgttggcaaa cagaatggca agcttgtgat gaattgcaaa tggccgcagc 2880

tactaaagct gaatttgccg cacttgaaga gctaaccagt catcagagtg atctatttag 2940

gcgtggttgg gacttacgtg gcagagtcga atacttgacg aaaattccga cctattacta 3000

tttataccgt gttggcggtg aaagcttagc agtagaaaag cagcgctctt gtcctaagtg 3060

tggcagtcaa gaatggctgc tcgataaacc attattggat atgttccatt ttcgctgtga 3120

cacctgccgc atcgtatcta atatctcttg ggaccattta taactcttcc gagtcttatc 3180

acactagagt ttagtcagca taaaaatggc gcttatattt caattaaaag aaatataagc 3240

gccattttca tcgatactat atatcagcag actattttcc gcgtaaatta gcccacatta 3300

atttcattct ttgccagatc cctggatgat ctagttgtgg catcgactct tcaataggtt 3360

taaccgcagg tgtaaccctt ggagtcaatt cgtttataaa ctcgtttaaa ctgtcactta 3420

atttaacgct ttgtacttca cctggaattt caatccatac gctgccatca ctattattaa 3480

ccgtcaacat tttatcttca tcatcaagaa taccaataaa ccaagtcggc tcttgcttaa 3540

gctttctctt catcattaaa tgaccaatga tgttttgttg taagtattca aaatcagttt 3600

gatcccacac ttggattagc tcaccttggc cccattgtga gtcaaaaaat agcggtgcag 3660

aaaaatgact gccaaaaaat ggattaattt ctgcagataa tgtcatttca agtgctgttt 3720

caacattagc aaattcacca ggttgttgac gtacaaccga ttgccaaaac actgcgccat 3780

cggagcccgc ttcggcgaca acacactcag acttttgtcc ttgcgcataa tatcttggct 3840

gttcaccaag cttatccatg taggcttgtt gatatttaga taaaaaaaga tctaaagcag 3900

gtaaagaaga cacttaagcc agttccaaaa tcagttataa taggggtcta ttttgacatg 3960

gaaaccgtat tgatgacaca acatcatgat ccctacagta acgcccccga actttctgaa 4020

ttaactttag gaaagtcgac cggttatcaa gagcagtatg atgcatcttt actacaagcg 4080

tgccgcgtaa attaaaccgt gatgctatcg gtctaaccaa tgagctacct tttcatggct 4140

gtgatatttg gactggctac gaactgtctt ggctaaatgc taaaggcaag ccaatgattg 4200

ctattgcaga ctttaaccta agttttgata gtaaaaatct gatcgagtct aagtcgttta 4260

agctgtattt aaacagctat aaccaaacac gatttgatag cgttcaagcg gttcaagaac 4320

gtttaactga agacttaagc gcctgtgccc aaggcacagt tacggtaaaa gtgattgaac 4380

ctaagcaatt taaccacctg agagtggttg atatgccagg tacctgcatt gacgatttag 4440

atattgaagt tgatgactat agctttaact ctgactatct caccgacagt gttgatgaca 4500

aagtcatggt tgctgaaacg ctaacgtcaa acttattgaa atcaaactgc ctaatcactt 4560

ctcagcctga ctggggtaca gtgatgatcc gttatcaagg gcctaagata gaccgtgaaa 4620

agctacttag atatctgatt tcatttagac agcacaatga atttcatgag cagtgtgttg 4680

agcgtatatt tgttgattta aagcactatt gccaatgtgc caaacttact gtctatgcac 4740

gttatacccg ccgtggtggt ttagatatca acccatatcg tagcgacttt gaaaaccctg 4800

cagaaaatca gcgcctagcg agacagtaat tgattgcagt acctacaaaa aacaatgcct 4860

ataagccaag cttatgggca tttttatatt atcaacttgt catcaaacct cagccgccaa 4920

gccttttagt tttatcgcta aattaagccg ctctctcagc caaatatttg caggattttg 4980

ctgtaattta tggctccaca ccatgaaata ctctatcggc tctaccgcaa aaggtaagtc 5040

aaatacctgt aagccaaaca gcttggcata ttcgtcagtg tgggcttttg acgcgatagc 5100

taacgcatca ctttttgagg caaccgacat catacttaat attgatgatt gctcgctgtg 5160

catttgcctt gccggtaaca cctgtttagt cagcaagtcg gcaacactta aattgtagcg 5220

gcgcatctta aaaataatat gcttttcatt aaagtattgc tcttgcgtca acccaccttg 5280

gatccttggg tgagcatttc gtgccacaca aactaattta tcctgcatta ctttttgact 5340

cttaaatgcc gcagattctg gcagccaaat atctaaggct aaatccacct tttctagttg 5400

taggtccatc tgcaactctt cttcaatgag cggcggctca cgaaatacaa tattaattgc 5460

agtgccctgt aacacttgct caatttgatc ttgcaagagt tgtattgccg actcgctggc 5520

atacacataa aaagttcgct cacttgaagt ggggtcaaat gcttcaaagc tagtcgcaac 5580

ttgctcaatt gttgacatag cgcccgcgag ctgttgataa agcgtcatcg cacttgcggt 5640

aggtttaact cccctaccca ctcgagtaaa caactcttct ccaacaatac tttttagcct 5700

cgaaatcgca ttactaaccg acgactgagt caaatccagc tcttctgccg cccggctaaa 5760

agatgaggtg cgatacaccg cagtaaaaac gcgaaataaa ttaagatcaa aagctttttg 5820

ctgcgacata aatcagctat ctccttatcc ttatccttat ccttataaaa agttagctcc 5880

agagcactct agctcaaaaa caactcagcg tattaagcca atattttggg aactcaatta 5940

atattcataa taaaagtatt cataatataa ataccaagtc ataatttagc cctaattatt 6000

aatcaattca agttacctat actggcctca attaagcaaa tgtctcatca gtctccctgc 6060

aactaaatgc aatattgaga cataaagctt tgaactgatt caatcttacg agggtaactt 6120

atgaaacaga ctctaatggc tatctcaatc atgtcgcttt tttcattcaa tgcgctagca 6180

gcgcaacatg aacatgacca catcactgtt gattacgaag ggaaagccgc aacagaacac 6240

accatagctc acaaccaagc tgtagctaaa acacttaact ttgccgacac gcgtgcattt 6300

gagcaatcgt ctaaaaatct agtcgccaag tttgataaag caactgccga tatattacgt 6360

gccgaatttg cttttattag cgatgaaatc cctgactcgg ttaacccgtc tctctaccgt 6420

caggctcagc ttaatatggt gcctaatggt ctgtataaag tgagcgatgg catttaccag 6480

gtccgcggta ccgacttatc taaccttaca cttatccgca gtgataacgg ttggatagca 6540

tacgatgttt tgttaaccaa agaagcagca aaagcctcac tacaatttgc gttaaagaat 6600

ctacctaaag atggcgattt acccgttgtt gcgatgattt actcccatag ccatgcggac 6660

cactttggcg gagctcgcgg tgttcaagag atgttccctg atgtcaaagt ctacggctca 6720

gataacatca ctaaagaaat tgtcgatgag aacgtacttg ccggtaacgc catgagccgc 6780

cgcgcagctt atcaatacgg cgcaacactg ggcaaacatg accacggtat tgttgatgct 6840

gcgctaggta aaggtctatc aaaaggtgaa atcacttacg tcgccccaga ctacacctta 6900

aacagtgaag gcaaatggga aacgctgacg attgatggtc tagagatggt gtttatggat 6960

gcctcgggca ccgaagctga gtcagaaatg atcacttata ttccctctaa aaaagcgctc 7020

tggacggcgg agcttaccta tcaaggtatg cacaacattt atacgctgcg cggcgctaaa 7080

gtacgtgatg cgctcaagtg gtcaaaagat atcaacgaaa tgatcaatgc ctttggtcaa 7140

gatgtcgaag tgctgtttgc ctcgcactct gcgccagtgt ggggtaacca agcgatcaac 7200

gatttcttac gcctacagcg tgataactac ggcctagtgc acaatcaaac cttgagactt 7260

gccaacgatg gtgtcggtat acaagatatt ggcgatgcga ttcaagacac gattccagag 7320

tctatctaca agacgtggca taccaatggt taccacggca cttatagcca taacgctaaa 7380

gcggtttata acaagtatct aggctacttc gatatgaacc cagccaacct taatccgctg 7440

ccaaccaagc aagaatctgc caagtttgtc gaatacatgg gcggcgcaga tgccgcaatt 7500

aagcgcgcta aagatgatta cgctcaaggt gaataccgct ttgttgcaac ggcattaaat 7560

aaggtggtga tggccgagcc agaaaatgac tccgctcgtc aattgctagc cgatacctat 7620

gagcaacttg gttatcaagc agaaggggct ggctggagaa acatttactt aactggcgca 7680

caagagctac gagtaggtat tcaagctggc gcgcctaaaa ccgcatcggc agatgtcatc 7740

agtgaaatgg acatgccgac tctatttgac ttcctcgcgg tgaagattga tagtcaacag 7800

gcggctaagc acggcttagt taagatgaat gttatcaccc ctgatactaa agatattctc 7860

tatattgagc taagcaacgg taacttaagc aacgcagtgg tcgacaaaga gcaagcagct 7920

gacgcaaacc ttatggttaa taaagctgac gttaaccgca tcttacttgg ccaagtaacc 7980

ctaaaagcgt tattagccag cggcgatgcc aagctcactg gtgataaaac ggcatttagt 8040

aaaatagccg atagcatggt cgagtttaca cctgacttcg aaatcgtacc aacgcctgtt 8100

aaatgaggca ttaatctcaa caagtgcaag ctagacataa aaatggggcg attagacgcc 8160

ccatttttta tgcaattttg aactagctag tcttagctga agctcgaaca acagctttaa 8220

aattcacttc ttctgctgca atacttattt gctgacactg accaatactc agtgcaaaac 8280

gataactatc atcaagatgg cccagtaaac aatgccaatt atcagcagcg ttcatttgct 8340

gttctttagc ctcaatcaaa cctaaaccag acttttgtgg ctcagcgtta ggcttattag 8400

aactcgactc tagtaaagca agaccaatat cttgttttaa caaaacctgt cgctgattaa 8460

gttgatgctc aaccttgtga tccgcaatag catcggaaat atcaacacaa tggctcaagc 8520

ttttaggtgc attaactcca agaaaagttt cgctcagtgc agagaagtca aacgcaaaag 8580

attttagcga taatgccagc ccaagtcctt tcgctttaat gtaagactcc ttgagcgccc 8640

acaaatcaaa aaagcggtct cgctgcaagg cctctggtaa cgctaacaag gctcgctttt 8700

ctgattcaga gaaataatga ctaagaatag agtggatatt ggtgctgtta cggcaacgct 8760

caatgtcgac gccaaactca atactagcag agtcagtttc ctccttgctt gcctgactgg 8820

cgcctttatt atcagcagtg caaatgccta ctaatagcca atctccacta tgactcacat 8880

taaagtggac cccggtttga gcaaattgcg catcactcaa tctaggctta cctttgtcgc 8940

catattcaaa gcgccattca ttggggcgta tttcactatg ttgtgacaat aaagcgcgca 9000

aatagcctct taccattaaa ccttgagttt tagcttcttg tttaatgtag cgattaacct 9060

taattaactc atcttcaggc agccatgact taaccaactc tgtagtctgg ttatcgcact 9120

cttgtattgt taacggacag aagtataagg aaatcaatcg agaagttagc aatttttcag 9180

gacactcttt aaagcaacaa acataacccc tatttttacc aatttaagat caaaactaaa 9240

gccaaaacta attgagaata gtgtcaaact agctttaaag gaaaaaaata taaaaagaac 9300

attatacttg tataaattat tttacacacc aaagccatga tcttcacaaa attagctccc 9360

tctccctaaa acaagattga ataaaaaaat aaaccttaac tttcatatag ataaaacaaa 9420

ccaatgggat aaagtatatt gaattcattt ttaaggaaaa attcaaattg aattcaagct 9480

cttcagtaaa agcatatttt gccgttagtg tgaaaaaaaa caaatttaaa aaccaacata 9540

gaacaaataa gcagacaata aaaccaaggc gcaacacaaa caacgcgctt acaattttca 9600

caaaaaagca acaagagtaa cgtttagtat ttggatatgg ttattgtaat tgagaatttt 9660

ataacaatta tattaaggga atgagtatgt ttttaaattc aaaactttcg cgctcagtca 9720

aacttgccat atccgcaggc ttaacagcct cgctagctat gcctgttttt gcagaagaaa 9780

ctgctgctga agaacaaata gaaagagtcg cagtgaccgg atcgcgaatc gctaaagcag 9840

agctaactca accagctcca gtcgtcagcc tttcagccga agaactgaca aaatttggta 9900

atcaagattt aggtagcgta ctagcagaat tacctgctat tggtgcaacc aacactatta 9960

ttggtaataa caatagcaac tcaagcgcag gtgttagctc agcagacttg cgtcgtctag 10020

gtgctaacag aaccttagta ttagtcaacg gtaagcgcta cgttgccggc caaccgggct 10080

cagctgaggt agatttgtca actataccaa ctagcatgat ctcgcgagtt gagattgtaa 10140

ccggcggtgc ttcagcaatt tatggttcgg acgctgtatc aggtgttatc aacgttatcc 10200

ttaaagaaga ctttgaaggc tttgagttta acgcacgtac tagcggttct actgaaagtg 10260

taggcactca agagcactct tttgacattt tgggtggtgc aaacgttgca gatggacgtg 10320

gtaatgtaac cttctacgca ggttatgaac gtacaaaaga agtcatggct accgacattc 10380

gccaattcga tgcttgggga acaattaaaa acgaagccga tggtggtgaa gatgatggta 10440

ttccagacag actacgtgta ccacgagttt attctgaaat gattaatgct accggtgtta 10500

tcaatgcatt tggtggtgga attggtcgct caacctttga cagtaacggc aatcctattg 10560

cacaacaaga acgtgatggg actaacagct ttgcatttgg ttcattccct aatggctgtg 10620

acacatgttt caacactgaa gcatacgaaa actatattcc aggggtagaa agaataaacg 10680

ttggctcatc attcaacttt gattttaccg ataacattca attttacact gacttcagat 10740

atgtaaagtc agatattcag caacaatttc agccttcatt ccgttttggt aacattaata 10800

tcaatgttga agataacgcc tttttgaatg acgacttgcg tcagcaaatg ctcgatgcgg 10860

gtcaaaccaa tgctagtttt gccaagtttt ttgatgaatt aggaaatcgc tcagcagaaa 10920

ataaacgcga acttttccgt tacgtaggtg gctttaaagg tggctttgat attagcgaaa 10980

ccatatttga ttacgacctt tactatgttt atggcgagac taataaccgt cgtaaaaccc 11040

ttaatgacct aattcctgat aactttgtcg cagctgtcga ctctgttatt gatcctgata 11100

ctggcttagc agcgtgtcgc tcacaagtag caagcgctca aggcgatgac tatacagatc 11160

ccgcgtctgt aaatggtagc gactgtgttg cttataaccc atttggcatg ggtcaagctt 11220

cagcagaagc ccgcgactgg gtttctgctg atgtgactcg tgaagacaaa ataactcaac 11280

aagtgattgg tggtactctc ggtaccgatt ctgaagaact atttgagctt caaggtggtg 11340

caatcgctat ggttgttggt tttgaatacc gtgaagaaac gtctggttca acaaccgatg 11400

aatttactaa agcaggtttc ttgacaagcg ctgcaacgcc agattcttat ggcgaatacg 11460

acgtgactga gtattttgtt gaggtgaaca tcccagtact aaaagaatta ccttttgcac 11520

atgagttgag ctttgacggt gcataccgta atgctgatta ctcacatgcc ggtaagactg 11580

aagcatggaa agctggtatg ttctactcac cattagagca acttgcatta cgtggtacgg 11640

taggtgaagc agtacgagca ccaaacattg cagaagcctt tagtccacgc tctcctggtt 11700

ttggccgcgt ttcagatcca tgtgatgcag ataacattaa tgacgatccg gatcgcgtgt 11760

caaactgtgc agcattgggg atccctccag gattccaagc taatgataac gtcagtgtag 11820

ataccttatc tggtggtaac ccagatctaa aacctgaaac atcaacatcc tttacaggtg 11880

gtcttgtttg gacaccaacg tttgctgaca atctatcatt cactgtcgat tattatgata 11940

ttcaaattga ggatgctatt ttgtcagtag ccacccagac tgtggctgat aactgtgttg 12000

actcaactgg cggacctgac accgacttct gtagtcaagt tgatcgtaat ccaacgacct 12060

atgatattga acttgttcgc tctggttatc taaatgccgc ggcattgaat accaaaggta 12120

ttgaatttca agctgcatac tcattagatc tagagtcttt caacgcgcct ggtgaactac 12180

gcttcaacct attggggaac caattacttg aactagaacg tcttgaattc caaaatcgtc 12240

ctgatgagat taatgatgaa aaaggcgaag taggtgatcc agagctgcag ttccgcctag 12300

gcatcgatta ccgtctagat gatctaagtg ttagctggaa cacgcgttat attgatagcg 12360

tagtaactta tgatgtctct gaaaatggtg gctctcctga agatttatat ccaggccaca 12420

taggctcaat gacaactcat gacttgagcg ctacatacta catcaatgag aacttcatga 12480

ttaacggtgg tgtacgtaac ctatttgacg cacttccacc tggatacact aacgatgcgc 12540

tatatgatct agttggtcgc cgtgcattcc taggtattaa ggtaatgatg taattaatta 12600

ttacgcctct aactaataaa aatgcaatct cttcgtagag attgcatttt tttatgaaat 12660

ccaatcttaa actggttctc cgagcatctt acgccttaaa aaccccgccc ctcaatgtaa 12720

cgccaaagtt aattgcttac acgcacttac acaaacgaac aatttcatta acacgagaca 12780

cagctcacgc tttttatttt acccttgatt ttactacata aaattgcgtt ttagcgcaca 12840

agtgttctcc caagctggtc gtatctgtaa ttattcagtc ccaggtgatt gtattgaccc 12900

ataagctcag gtagtctgct ctgccattag ctaaacaata ttgacaaaat ggcgataaaa 12960

tgtggcttag cgctaagttc accgtaagtt ttatcggcat taagtcccaa cagattatta 13020

acggaaaccc gctaaactga tggcaaaaat aaatagtgaa cacttggatg aagctactat 13080

tacttcgaat aagtgtacgc aaacagagac tgaggctcgg catagaaatg ccactacaac 13140

acctgagatg cgccgattca tacaagagtc ggatctcagt gttagccaac tgtctaaaat 13200

attaaatatc agtgaagcta ccgtacgtaa gtggcgcaag cgtgactctg tcgaaaactg 13260

tcctaatacc ccgcaccatc tcaataccac gctaacccct ttgcaagaat atgtggttgt 13320

gggcctgcgt tatcaattga aaatgccatt agacagattg ctcaaagcaa cccaagagtt 13380

tatcaatcca aacgtgtcgc gctcaggttt agcaagatgt ttgaagcgtt atggcgtttc 13440

acgggtgagt gatatccaaa gcccacacgt accaatgcgc tactttaatc aaattccagt 13500

cactcaaggc agcgatgtgc aaacctacac cctgcactat gaaacgctgg caaaaacctt 13560

agccttacct agtaccgatg gtgacaatgt ggtgcaagtg gtgtctctca ccattccacc 13620

aaagttaacc gaagaagcac ccagttcaat tttgctcggc attgatcctc atagcgactg 13680

gatctatctc gacatatacc aagatggcaa tacacaagcc acgaatagat atatggctta 13740

tgtgctaaaa cacgggccat tccatttacg aaagttactc gtgcgtaact atcacacctt 13800

tttacagcgc tttcctggag cgacgcaaaa tcgccgcccc tctaaagata tgcctgaaac 13860

aatcaacaag acgcctgaaa cacaggcacc cagtggagac tcataatgag ccagacctct 13920

aaacctacaa actcagcaac tgagcaagca caagactcac aagctgactc tcgtttaaat 13980

aaacgactaa aagatatgcc aattgctatt gttggcatgg cgagtatttt tgcaaactct 14040

cgctatttga ataagttttg ggacttaatc agcgaaaaaa ttgatgcgat tactgaatta 14100

ccatcaactc actggcagcc tgaagaatat tacgacgcag ataaaaccgc agcagacaaa 14160

agctactgta aacgtggtgg ctttttgcca gatgtagact tcaacccaat ggagtttggc 14220

ctgccgccaa acattttgga actgaccgat tcatcgcaac tattatcact catcgttgct 14280

aaagaagtgt tggctgatgc taacttacct gagaattacg accgcgataa aattggtatc 14340

accttaggtg tcggcggtgg tcaaaaaatt agccacagcc taacagcgcg tctgcaatac 14400

ccagtattga agaaagtatt cgccaatagc ggcattagtg acaccgacag cgaaatgctt 14460

atcaagaaat tccaagacca atatgtacac tgggaagaaa actcgttccc aggttcactt 14520

ggtaacgtta ttgcgggccg tatcgccaac cgcttcgatt ttggcggcat gaactgtgtg 14580

gttgatgctg cctgtgctgg atcacttgct gctatgcgta tggcgctaac agagctaact 14640

gaaggtcgct ctgaaatgat gatcaccggt ggtgtgtgta ctgataactc accctctatg 14700

tatatgagct tttcaaaaac gcccgccttt accactaacg aaaccattca gccatttgat 14760

atcgactcaa aaggcatgat gattggtgaa ggtattggca tggtggcgct aaagcgtctt 14820

gaagatgcag agcgcgatgg cgaccgcatt tactctgtaa ttaaaggtgt gggtgcatca 14880

tctgacggta agtttaaatc aatctatgcc cctcgcccat caggccaagc taaagcactt 14940

aaccgtgcct atgatgacgc aggttttgcg ccgcatacct taggtctaat tgaagctcac 15000

ggaacaggta ctgcagcagg tgacgcggca gagtttgccg gcctttgctc agtatttgct 15060

gaaggcaacg ataccaagca acacattgcg ctaggttcag ttaaatcaca aattggtcat 15120

actaaatcaa ctgcaggtac agcaggttta attaaagctg ctcttgcttt gcatcacaag 15180

gtactgccgc cgaccattaa cgttagtcag ccaagcccta aacttgatat cgaaaactca 15240

ccgttttatc taaacactga gactcgtcca tggttaccac gtgttgatgg tacgccgcgc 15300

cgcgcgggta ttagctcatt tggttttggt ggcactaact tccattttgt actagaagag 15360

tacaaccaag aacacagccg tactgatagc gaaaaagcta agtatcgtca acgccaagtg 15420

gcgcaaagct tccttgttag cgcaagcgat aaagcatcgc taattaacga gttaaacgta 15480

ctagcagcat ctgcaagcca agctgagttt atcctcaaag atgcagcagc aaactatggc 15540

gtacgtgagc ttgataaaaa tgcaccacgg atcggtttag ttgcaaacac agctgaagag 15600

ttagcaggcc taattaagca agcacttgcc aaactagcag ctagcgatga taacgcatgg 15660

cagctacctg gtggcactag ctaccgcgcc gctgcagtag aaggtaaagt tgccgcactg 15720

tttgctggcc aaggttcaca atatctcaat atgggccgtg accttacttg ttattaccca 15780

gagatgcgtc agcaatttgt aactgcagat aaagtatttg ccgcaaatga taaaacgccg 15840

ttatcgcaaa ctctgtatcc aaagcctgta tttaataaag atgaattaaa ggctcaagaa 15900

gccattttga ccaataccgc caatgcccaa agcgcaattg gtgcgatttc aatgggtcaa 15960

tacgatttgt ttactgcggc tggctttaat gccgacatgg ttgcaggcca tagctttggt 16020

gagctaagtg cactgtgtgc tgcaggtgtt atttcagctg atgactacta caagctggct 16080

tttgctcgtg gtgaggctat ggcaacaaaa gcaccggcta aagacggcgt tgaagcagat 16140

gcaggagcaa tgtttgcaat cataaccaag agtgctgcag accttgaaac cgttgaagcc 16200

accatcgcta aatttgatgg ggtgaaagtc gctaactata acgcgccaac gcaatcagta 16260

attgcaggcc caacagcaac taccgctgat gcggctaaag cgctaactga gcttggttac 16320

aaagcgatta acctgccagt atcaggtgca ttccacactg aacttgttgg tcacgctcaa 16380

gcgccatttg ctaaagcgat tgacgcagcc aaatttacta aaacaagccg agcactttac 16440

tcaaatgcaa ctggcggact ttatgaaagc actgctgcaa agattaaagc ctcgtttaag 16500

aaacatatgc ttcaatcagt gcgctttact agccagctag aagccatgta caacgacggc 16560

gcccgtgtat ttgttgaatt tggtccaaag aacatcttac aaaaattagt tcaaggcacg 16620

cttgtcaaca ctgaaaatga agtttgcact atctctatca accctaatcc taaagttgat 16680

agtgatctgc agcttaagca agcagcaatg cagctagcgg ttactggtgt ggtactcagt 16740

gaaattgacc cataccaagc cgatattgcc gcaccagcga aaaagtcgcc aatgagcatt 16800

tcgcttaatg ctgctaacca tatcagcaaa gcaactcgcg ctaagatggc caagtcttta 16860

gagacaggta tcgtcacctc gcaaatagaa catgttattg aagaaaaaat cgttgaagtt 16920

gagaaactgg ttgaagtcga aaagatcgtc gaaaaagtgg ttgaagtaga gaaagttgtt 16980

gaggttgaag ctcctgttaa ttcagtgcaa gccaatgcaa ttcaaacccg ttcagttgtc 17040

gctccagtaa tagagaacca agtcgtgtct aaaaacagta agccagcagt ccagagcatt 17100

agtggtgatg cactcagcaa cttttttgct gcacagcagc aaaccgcaca gttgcatcag 17160

cagttcttag ctattccgca gcaatatggt gagacgttca ctacgctgat gaccgagcaa 17220

gctaaactgg caagttctgg tgttgcaatt ccagagagtc tgcaacgctc aatggagcaa 17280

ttccaccaac tacaagcgca aacactacaa agccacaccc agttccttga gatgcaagcg 17340

ggtagcaaca ttgcagcgtt aaacctactc aatagcagcc aagcaactta cgctccagcc 17400

attcacaatg aagcgattca aagccaagtg gttcaaagcc aaactgcagt ccagccagta 17460

atttcaacac aagttaacca tgtgtcagag cagccaactc aagctccagc tccaaaagcg 17520

cagccagcac ctgtgacaac tgcagttcaa actgctccgg cacaagttgt tcgtcaagcc 17580

gcaccagttc aagccgctat tgaaccgatt aatacaagtg ttgcgactac aacgccttca 17640

gccttcagcg ccgaaacagc cctgagcgca acaaaagtcc aagccactat gcttgaagtg 17700

gttgctgaga aaaccggtta cccaactgaa atgctagagc ttgaaatgga tatggaagcc 17760

gatttaggca tcgattctat caagcgtgta gaaattcttg gcacagtaca agatgagcta 17820

ccgggtctac ctgagcttag ccctgaagat ctagctgagt gtcgaacgct aggcgaaatc 17880

gttgactata tgggcagtaa actgccggct gaaggctcta tgaattctca gctgtctaca 17940

ggttccgcag ctgcgactcc tgcagcgaat ggtctttctg cggagaaagt tcaagcgact 18000

atgatgtctg tggttgccga aaagactggc tacccaactg aaatgctaga gcttgaaatg 18060

gatatggaag ccgatttagg catagattct atcaagcgcg ttgaaattct tggcacagta 18120

caagatgagc taccgggtct acctgagctt agccctgaag atctagctga gtgtcgtact 18180

ctaggcgaaa tcgttgacta tatgaactct aaactcgctg acggctctaa gctgccggct 18240

gaaggctcta tgaattctca gctgtctaca agtgccgcag ctgcgactcc tgcagcgaat 18300

ggtctctctg cggagaaagt tcaagcgact atgatgtctg tggttgccga aaagactggc 18360

tacccaactg aaatgctaga acttgaaatg gatatggaag ctgaccttgg catcgattca 18420

atcaagcgcg ttgaaattct tggcacagta caagatgagc taccgggttt acctgagcta 18480

aatccagaag atttggcaga gtgtcgtact cttggcgaaa tcgtgactta tatgaactct 18540

aaactcgctg acggctctaa gctgccagct gaaggctcta tgcactatca gctgtctaca 18600

agtaccgctg ctgcgactcc tgtagcgaat ggtctctctg cagaaaaagt tcaagcgacc 18660

atgatgtctg tagttgcaga taaaactggc tacccaactg aaatgcttga acttgaaatg 18720

gatatggaag ccgatttagg tatcgattct atcaagcgcg ttgaaattct tggcacagta 18780

caagatgagc taccgggttt acctgagcta aatccagaag atctagcaga gtgtcgcacc 18840

ctaggcgaaa tcgttgacta tatgggcagt aaactgccgg ctgaaggctc tgctaataca 18900

agtgccgctg cgtctcttaa tgttagtgcc gttgcggcgc ctcaagctgc tgcgactcct 18960

gtatcgaacg gtctctctgc agagaaagtg caaagcacta tgatgtcagt agttgcagaa 19020

aagaccggct acccaactga aatgctagaa cttggcatgg atatggaagc cgatttaggt 19080

atcgactcaa ttaaacgcgt tgagattctt ggcacagtac aagatgagct accgggtcta 19140

ccagagctta atcctgaaga tttagctgag tgccgtacgc tgggcgaaat cgttgactat 19200

atgaactcta agctggctga cggctctaag cttccagctg aaggctctgc taatacaagt 19260

gccactgctg cgactcctgc agtgaatggt ctttctgctg acaaggtaca ggcgactatg 19320

atgtctgtag ttgctgaaaa gaccggctac ccaactgaaa tgctagaact tggcatggat 19380

atggaagcag accttggtat tgattctatt aagcgcgttg aaattcttgg cacagtacaa 19440

gatgagctcc caggtttacc tgagcttaat cctgaagatc tcgctgagtg ccgcacgctt 19500

ggcgaaatcg ttagctatat gaactctcaa ctggctgatg gctctaaact ttctacaagt 19560

gcggctgaag gctctgctga tacaagtgct gcaaatgctg caaagccggc agcaatttcg 19620

gcagaaccaa gtgttgagct tcctcctcat agcgaggtag cgctaaaaaa gcttaatgcg 19680

gcgaacaagc tagaaaattg tttcgccgca gacgcaagtg ttgtgattaa cgatgatggt 19740

cacaacgcag gcgttttagc tgagaaactt attaaacaag gcctaaaagt agccgttgtg 19800

cgtttaccga aaggtcagcc tcaatcgcca ctttcaagcg atgttgctag ctttgagctt 19860

gcctcaagcc aagaatctga gcttgaagcc agtatcactg cagttatcgc gcagattgaa 19920

actcaggttg gcgctattgg tggctttatt cacttgcaac cagaagcgaa tacagaagag 19980

caaacggcag taaacctaga tgcgcaaagt tttactcacg ttagcaatgc gttcttgtgg 20040

gccaaattat tgcaaccaaa gctcgttgct ggagcagatg cgcgtcgctg ttttgtaaca 20100

gtaagccgta tcgacggtgg ctttggttac ctaaatactg acgccctaaa agatgctgag 20160

ctaaaccaag cagcattagc tggtttaact aaaaccttaa gccatgaatg gccacaagtg 20220

ttctgtcgcg cgctagatat tgcaacagat gttgatgcaa cccatcttgc tgatgcaatc 20280

accagtgaac tatttgatag ccaagctcag ctacctgaag tgggcttaag cttaattgat 20340

ggcaaagtta accgcgtaac tctagttgct gctgaagctg cagataaaac agcaaaagca 20400

gagcttaaca gcacagataa aatcttagtg actggtgggg caaaaggggt gacatttgaa 20460

tgtgcactgg cattagcatc tcgcagccag tctcacttta tcttagctgg gcgcagtgaa 20520

ttacaagctt taccaagctg ggctgagggt aagcaaacta gcgagctaaa atcagctgca 20580

atcgcacata ttatttctac tggtcaaaag ccaacgccta agcaagttga agccgctgtg 20640

tggccagtgc aaagcagcat tgaaattaat gccgccctag ccgcctttaa caaagttggc 20700

gcctcagctg aatacgtcag catggatgtt accgatagcg ccgcaatcac agcagcactt 20760

aatggtcgct caaatgagat caccggtctt attcatggcg caggtgtact agccgacaag 20820

catattcaag acaagactct tgctgaactt gctaaagttt atggcactaa agtcaacggc 20880

ctaaaagcgc tgctcgcggc acttgagcca agcaaaatta aattacttgc tatgttctca 20940

tctgcagcag gtttttacgg taatatcggc caaagcgatt acgcgatgtc gaacgatatt 21000

cttaacaagg cagcgctgca gttcaccgct cgcaacccac aagctaaagt catgagcttt 21060

aactggggtc cttgggatgg cggcatggtt aacccagcgc ttaaaaagat gtttaccgag 21120

cgtggtgtgt acgttattcc actaaaagca ggtgcagagc tatttgccac tcagctattg 21180

gctgaaactg gcgtgcagtt gctcattggt acgtcaatgc aaggtggcag cgacactaaa 21240

gcaactgaga ctgcttctgt aaaaaagctt aatgcgggtg aggtgctaag tgcatcgcat 21300

ccgcgtgctg gtgcacaaaa aacaccacta caagctgtca ctgcaacgcg tctgttaacc 21360

ccaagtgcca tggtcttcat tgaagatcac cgcattggcg gtaacagtgt gttgccaacg 21420

gtatgcgcca tcgactggat gcgtgaagcg gcaagcgaca tgcttggcgc tcaagttaag 21480

gtacttgatt acaagctatt aaaaggcatt gtatttgaga ctgatgagcc gcaagagtta 21540

acacttgagc taacgccaga cgattcagac gaagctacgc tacaagcatt aatcagctgt 21600

aatgggcgtc cgcaatacaa ggcgacgctt atcagtgata atgccgatat taagcaactt 21660

aacaagcagt ttgatttaag cgctaaggcg attaccacag caaaagagct ttatagcaac 21720

ggcaccttgt tccacggtcc gcgtctacaa gggatccaat ctgtagtgca gttcgatgat 21780

caaggcttaa ttgctaaagt cgctctgcct aaggttgaac ttagcgattg tggtgagttc 21840

ttgccgcaaa cccacatggg tggcagtcaa ccttttgctg aggacttgct attacaagct 21900

atgctggttt gggctcgcct taaaactggc tcggcaagtt tgccatcaag cattggtgag 21960

tttacctcat accaaccaat ggcctttggt gaaactggta ccatagagct tgaagtgatt 22020

aagcacaaca aacgctcact tgaagcgaat gttgcgctat atcgtgacaa cggcgagtta 22080

agtgccatgt ttaagtcagc taaaatcacc attagcaaaa gcttaaattc agcattttta 22140

cctgctgtct tagcaaacga cagtgaggcg aattagtgga acaaacgcct aaagctagtg 22200

cgatgccgct gcgcatcgca cttatcttac tgccaacacc gcagtttgaa gttaactctg 22260

tcgaccagtc agtattagcc agctatcaaa cactgcagcc tgagctaaat gccctgctta 22320

atagtgcgcc gacacctgaa atgctcagca tcactatctc agatgatagc gatgcaaaca 22380

gctttgagtc gcagctaaat gctgcgacca acgcaattaa caatggctat atcgtcaagc 22440

ttgctacggc aactcacgct ttgttaatgc tgcctgcatt aaaagcggcg caaatgcgga 22500

tccatcctca tgcgcagctt gccgctatgc agcaagctaa atcgacgcca atgagtcaag 22560

tatctggtga gctaaagctt ggcgctaatg cgctaagcct agctcagact aatgcgctgt 22620

ctcatgcttt aagccaagcc aagcgtaact taactgatgt cagcgtgaat gagtgttttg 22680

agaacctcaa aagtgaacag cagttcacag aggtttattc gcttattcag caacttgcta 22740

gccgcaccca tgtgagaaaa gaggttaatc aaggtgtgga acttggccct aaacaagcca 22800

aaagccacta ttggtttagc gaatttcacc aaaaccgtgt tgctgccatc aactttatta 22860

atggccaaca agcaaccagc tatgtgctta ctcaaggttc aggattgtta gctgcgaaat 22920

caatgctaaa ccagcaaaga ttaatgttta tcttgccggg taacagtcag caacaaataa 22980

ccgcatcaat aactcagtta atgcagcaat tagagcgttt gcaggtaact gaggttaatg 23040

agctttctct agaatgccaa ctagagctgc tcagcataat gtatgacaac ttagtcaacg 23100

cagacaaact cactactcgc gatagtaagc ccgcttatca ggctgtgatt caagcaagct 23160

ctgttagcgc tgcaaagcaa gagttaagcg cgcttaacga tgcactcaca gcgctgtttg 23220

ctgagcaaac aaacgccaca tcaacgaata aaggcttaat ccaatacaaa acaccggcgg 23280

gcagttactt aaccctaaca ccgcttggca gcaacaatga caacgcccaa gcgggtcttg 23340

cttttgtcta tccgggtgtg ggaacggttt acgccgatat gcttaatgag ctgcatcagt 23400

acttccctgc gctttacgcc aaacttgagc gtgaaggcga tttaaaggcg atgctacaag 23460

cagaagatat ctatcatctt gaccctaaac atgctgccca aatgagctta ggtgacttag 23520

ccattgctgg cgtggggagc agctacctgt taactcagct gctcaccgat gagtttaata 23580

ttaagcctaa ttttgcatta ggttactcaa tgggtgaagc atcaatgtgg gcaagcttag 23640

gcgtatggca aaacccgcat gcgctgatca gcaaaaccca aaccgacccg ctatttactt 23700

ctgctatttc cggcaaattg accgcggtta gacaagcttg gcagcttgat gataccgcag 23760

cggaaatcca gtggaatagc tttgtggtta gaagtgaagc agcgccgatt gaagccttgc 23820

taaaagatta cccacacgct tacctcgcga ttattcaagg ggatacctgc gtaatcgctg 23880

gctgtgaaat ccaatgtaaa gcgctacttg cagcactggg taaacgcggt attgcagcta 23940

atcgtgtaac ggcgatgcat acgcagcctg cgatgcaaga gcatcaaaat gtgatggatt 24000

tttatctgca accgttaaaa gcagagcttc ctagtgaaat aagctttatc agcgccgctg 24060

atttaactgc caagcaaacg gtgagtgagc aagcacttag cagccaagtc gttgctcagt 24120

ctattgccga caccttctgc caaaccttgg actttaccgc gctagtacat cacgcccaac 24180

atcaaggcgc taagctgttt gttgaaattg gcgcggatag acaaaactgc accttgatag 24240

acaagattgt taaacaagat ggtgccagca gtgtacaaca tcaaccttgt tgcacagtgc 24300

ctatgaacgc aaaaggtagc caagatatta ccagcgtgat taaagcgctt ggccaattaa 24360

ttagccatca ggtgccatta tcggtgcaac catttattga tggactcaag cgcgagctaa 24420

cactttgcca attgaccagc caacagctgg cagcacatgc aaatgttgac agcaagtttg 24480

agtctaacca agaccattta cttcaagggg aagtctaatg tcattaccag acaatgcttc 24540

taaccacctt tctgccaacc agaaaggcgc atctcaggca agtaaaacca gtaagcaaag 24600

caaaatcgcc attgtcggtt tagccactct gtatccagac gctaaaaccc cgcaagaatt 24660

ttggcagaat ttgctggata aacgcgactc tcgcagcacc ttaactaacg aaaaactcgg 24720

cgctaacagc caagattatc aaggtgtgca aggccaatct gaccgttttt attgtaataa 24780

aggcggctac attgagaact tcagctttaa tgctgcaggc tacaaattgc cggagcaaag 24840

cttaaatggc ttggacgaca gcttcctttg ggcgctcgat actagccgta acgcactaat 24900

tgatgctggt attgatatca acggcgctga tttaagccgc gcaggtgtag tcatgggcgc 24960

gctgtcgttc ccaactaccc gctcaaacga tctgtttttg ccaatttatc acagcgccgt 25020

tgaaaaagcc ctgcaagata aactaggcgt aaaggcattt aagctaagcc caactaatgc 25080

tcataccgct cgcgcggcaa atgagagcag cctaaatgca gccaatggtg ccattgccca 25140

taacagctca aaagtggtgg ccgatgcact tggccttggc ggcgcacaac taagcctaga 25200

tgctgcctgt gctagttcgg tttactcatt aaagcttgcc tgcgattacc taagcactgg 25260

caaagccgat atcatgctag caggcgcagt atctggcgcg gatcctttct ttattaatat 25320

gggattctca atcttccacg cctacccaga ccatggtatc tcagtaccgt ttgatgccag 25380

cagtaaaggt ttgtttgctg gcgaaggcgc tggcgtatta gtgcttaaac gtcttgaaga 25440

tgccgagcgc gacaatgaca aaatctatgc ggttgttagc ggcgtaggtc tatcaaacga 25500

cggtaaaggc cagtttgtat taagccctaa tccaaaaggt caggtgaagg cctttgaacg 25560

tgcttatgct gccagtgaca ttgagccaaa agacattgaa gtgattgagt gccacgcaac 25620

aggcacaccg cttggcgata aaattgagct cacttcaatg gaaaccttct ttgaagacaa 25680

gctgcaaggc accgatgcac cgttaattgg ctcagctaag tctaacttag gccacctatt 25740

aactgcagcg catgcgggga tcatgaagat gatcttcgcc atgaaagaag gttacctgcc 25800

gccaagtatc aatattagtg atgctatcgc ttcgccgaaa aaactcttcg gtaaaccaac 25860

cctgcctagc atggttcaag gctggccaga taagccatcg aataatcatt ttggtgtaag 25920

aacccgtcac gcaggcgtat cggtatttgg ctttggtggc tgtaacgccc atctgttgct 25980

tgagtcatac aacggcaaag gaacagtaaa ggcagaagcc actcaagtac cgcgtcaagc 26040

tgagccgcta aaagtggttg gccttgcctc gcactttggg cctcttagca gcattaatgc 26100

actcaacaat gctgtgaccc aagatgggaa tggctttatc gaactgccga aaaagcgctg 26160

gaaaggcctt gaaaagcaca gtgaactgtt agctgaattt ggcttagcat ctgcgccaaa 26220

aggtgcttat gttgataact tcgagctgga ctttttacgc tttaaactgc cgccaaacga 26280

agatgaccgt ttgatctcac agcagctaat gctaatgcga gtaacagacg aagccattcg 26340

tgatgccaag cttgagccgg ggcaaaaagt agctgtatta gtggcaatgg aaactgagct 26400

tgaactgcat cagttccgcg gccgggttaa cttgcatact caattagcgc aaagtcttgc 26460

cgccatgggc gtgagtttat caacggatga ataccaagcg cttgaagcca tcgccatgga 26520

cagcgtgctt gatgctgcca agctcaatca gtacaccagc tttattggta atattatggc 26580

gtcacgcgtg gcgtcactat gggactttaa tggcccagcc ttcactattt cagcagcaga 26640

gcaatctgtg agccgctgta tcgatgtggc gcaaaacctc atcatggagg ataacctaga 26700

tgcggtggtg attgcagcgg tcgatctctc tggtagcttt gagcaagtca ttcttaaaaa 26760

tgccattgca cctgtagcca ttgagccaaa cctcgaagca agccttaatc caacatcagc 26820

aagctggaat gtcggtgaag gtgctggcgc ggtcgtgctt gttaaaaatg aagctacatc 26880

gggctgctca tacggccaaa ttgatgcact tggctttgct aaaactgccg aaacagcgtt 26940

ggctaccgac aagctactga gccaaactgc cacagacttt aataaggtta aagtgattga 27000

aactatggca gcgcctgcta gccaaattca attagcgcca atagttagct ctcaagtgac 27060

tcacactgct gcagagcagc gtgttggtca ctgctttgct gcagcgggta tggcaagcct 27120

attacacggc ttacttaact taaatactgt agcccaaacc aataaagcca attgcgcgct 27180

tatcaacaat atcagtgaaa accaattatc acagctgttg attagccaaa cagcgagcga 27240

acaacaagca ttaaccgcgc gtttaagcaa tgagcttaaa tccgatgcta aacaccaact 27300

ggttaagcaa gtcaccttag gtggccgtga tatctaccag catattgttg atacaccgct 27360

tgcaagcctt gaaagcatta ctcagaaatt ggcgcaagcg acagcatcga cagtggtcaa 27420

ccaagttaaa cctattaagg ccgctggctc agtcgaaatg gctaactcat tcgaaacgga 27480

aagctcagca gagccacaaa taacaattgc agcacaacag actgcaaaca ttggcgtcac 27540

cgctcaggca accaaacgtg aattaggtac cccaccaatg acaacaaata ccattgctaa 27600

tacagcaaat aatttagaca agactcttga gactgttgct ggcaatactg ttgctagcaa 27660

ggttggctct ggcgacatag tcaattttca acagaaccaa caattggctc aacaagctca 27720

cctcgccttt cttgaaagcc gcagtgcggg tatgaaggtg gctgatgctt tattgaagca 27780

acagctagct caagtaacag gccaaactat cgataatcag gccctcgata ctcaagccgt 27840

cgatactcaa acaagcgaga atgtagcgat tgccgcagaa tcaccagttc aagttacaac 27900

acctgttcaa gttacaacac ctgttcaaat cagtgttgtg gagttaaaac cagatcacgc 27960

taatgtgcca ccatacacgc cgccagtgcc tgcattaaag ccgtgtatct ggaactatgc 28020

cgatttagtt gagtacgcag aaggcgatat cgccaaggta tttggcagtg attatgccat 28080

tatcgacagc tactcgcgcc gcgtacgtct accgaccact gactacctgt tggtatcgcg 28140

cgtgaccaaa cttgatgcga ccatcaatca atttaagcca tgctcaatga ccactgagta 28200

cgacatccct gttgatgcgc cgtacttagt agacggacaa atcccttggg cggtagcagt 28260

agaatcaggc caatgtgact tgatgcttat tagctatctc ggtatcgact ttgagaacaa 28320

aggcgagcgg gtttatcgac tactcgattg taccctcacc ttcctaggcg acttgccacg 28380

tggcggagat accctacgtt acgacattaa gatcaataac tatgctcgca acggcgacac 28440

cctgctgttc ttcttctcgt atgagtgttt tgttggcgac aagatgatcc tcaagatgga 28500

tggcggctgc gctggcttct tcactgatga agagcttgcc gacggtaaag gcgtgattcg 28560

cacagaagaa gagattaaag ctcgcagcct agtgcaaaag caacgcttta atccgttact 28620

agattgtcct aaaacccaat ttagttatgg tgatattcat aagctattaa ctgctgatat 28680

tgagggttgt tttggcccaa gccacagtgg cgtccaccag ccgtcacttt gtttcgcatc 28740

tgaaaaattc ttgatgattg aacaagtcag caaggttgat cgcactggcg gtacttgggg 28800

acttggctta attgagggtc ataagcagct tgaagcagac cactggtact tcccatgtca 28860

tttcaagggc gaccaagtga tggctggctc gctaatggct gaaggttgtg gccagttatt 28920

gcagttctat atgctgcacc ttggtatgca tacccaaact aaaaatggtc gtttccaacc 28980

tcttgaaaac gcctcacagc aagtacgctg tcgcggtcaa gtgctgccac aatcaggcgt 29040

gctaacttac cgtatggaag tgactgaaat cggtttcagt ccacgcccat atgctaaagc 29100

taacatcgat atcttgctta atggcaaagc ggtagtggat ttccaaaacc taggggtgat 29160

gataaaagag gaagatgagt gtactcgtta tccacttttg actgaatcaa caacggctag 29220

cactgcacaa gtaaacgctc aaacaagtgc gaaaaaggta tacaagccag catcagtcaa 29280

tgcgccatta atggcacaaa ttcctgatct gactaaagag ccaaacaagg gcgttattcc 29340

gatttcccat gttgaagcac caattacgcc agactacccg aaccgtgtac ctgatacagt 29400

gccattcacg ccgtatcaca tgtttgagtt tgctacaggc aatatcgaaa actgtttcgg 29460

gccagagttc tcaatctatc gcggcatgat cccaccacgt acaccatgcg gtgacttaca 29520

agtgaccaca cgtgtgattg aagttaacgg taagcgtggc gactttaaaa agccatcatc 29580

gtgtatcgct gaatatgaag tgcctgcaga tgcgtggtat ttcgataaaa acagccacgg 29640

cgcagtgatg ccatattcaa ttttaatgga gatctcactg caacctaacg gctttatctc 29700

aggttacatg ggcacaaccc taggcttccc tggccttgag ctgttcttcc gtaacttaga 29760

cggtagcggt gagttactac gtgaagtaga tttacgtggt aaaaccatcc gtaacgactc 29820

acgtttatta tcaacagtga tggccggcac taacatcatc caaagcttta gcttcgagct 29880

aagcactgac ggtgagcctt tctatcgcgg cactgcggta tttggctatt ttaaaggtga 29940

cgcacttaaa gatcagctag gcctagataa cggtaaagtc actcagccat ggcatgtagc 30000

taacggcgtt gctgcaagca ctaaggtgaa cctgcttgat aagagctgcc gtcactttaa 30060

tgcgccagct aaccagccac actatcgtct agccggtggt cagctgaact ttatcgacag 30120

tgttgaaatt gttgataatg gcggcaccga aggtttaggt tacttgtatg ccgagcgcac 30180

cattgaccca agtgattggt tcttccagtt ccacttccac caagatccgg ttatgccagg 30240

ctccttaggt gttgaagcaa ttattgaaac catgcaagct tacgctatta gtaaagactt 30300

gggcgcagat ttcaaaaatc ctaagtttgg tcagatttta tcgaacatca agtggaagta 30360

tcgcggtcaa atcaatccgc tgaacaagca gatgtctatg gatgtcagca ttacttcaat 30420

caaagatgaa gacggtaaga aagtcatcac aggtaatgcc agcttgagta aagatggtct 30480

gcgcatatac gaggtcttcg atatagctat cagcatcgaa gaatctgtat aaatcggagt 30540

gactgtctgg ctattttact caatttctgt gtcaaaagtg ctcacctata ttcataggct 30600

gcgcgctttt ttctggaaat tgagcaaaag tatctgcgtc ctaactcgat ttataagaat 30660

ggtttaattg aaaagaacaa cagctaagag ccgcaagctc aatataaata attaagggtc 30720

ttacaaataa tgaatcctac agcaactaac gaaatgcttt ctccgtggcc atgggctgtg 30780

acagagtcaa atatcagttt tgacgtgcaa gtgatggaac aacaacttaa agattttagc 30840

cgggcatgtt acgtggtcaa tcatgccgac cacggctttg gtattgcgca aactgccgat 30900

atcgtgactg aacaagcggc aaacagcaca gatttacctg ttagtgcttt tactcctgca 30960

ttaggtaccg aaagcctagg cgacaataat ttccgccgcg ttcacggcgt taaatacgct 31020

tattacgcag gcgctatggc aaacggtatt tcatctgaag agctagtgat tgccctaggt 31080

caagctggca ttttgtgtgg ttcgtttgga gcagccggtc ttattccaag tcgcgttgaa 31140

gcggcaatta accgtattca agcagcgctg ccaaatggcc cttatatgtt taaccttatc 31200

catagtccta gcgagccagc attagagcgt ggcagcgtag agctattttt aaagcataag 31260

gtacgcaccg ttgaagcatc agctttctta ggtctaacac cacaaatcgt ctattaccgt 31320

gcagcaggat tgagccgaga cgcacaaggt aaagttgtgg ttggtaacaa ggttatcgct 31380

aaagtaagtc gcaccgaagt ggctgaaaag tttatgatgc cagcgcccgc aaaaatgcta 31440

caaaaactag ttgatgacgg ttcaattacc gctgagcaaa tggagctggc gcaacttgta 31500

cctatggctg acgacatcac tgcagaggcc gattcaggtg gccatactga taaccgtcca 31560

ttagtaacat tgctgccaac cattttagcg ctgaaagaag aaattcaagc taaataccaa 31620

tacgacactc ctattcgtgt cggttgtggt ggcggtgtgg gtacgcctga tgcagcgctg 31680

gcaacgttta acatgggcgc ggcgtatatt gttaccggct ctatcaacca agcttgtgtt 31740

gaagcgggcg caagtgatca cactcgtaaa ttacttgcca ccactgaaat ggccgatgtg 31800

actatggcac cagctgcaga tatgttcgag atgggcgtaa aactgcaggt ggttaagcgc 31860

ggcacgctat tcccaatgcg cgctaacaag ctatatgaga tctacacccg ttacgattca 31920

atcgaagcga tcccattaga cgagcgtgaa aagcttgaga aacaagtatt ccgctcaagc 31980

ctagatgaaa tatgggcagg tacagtggcg cactttaacg agcgcgaccc taagcaaatc 32040

gaacgcgcag agggtaaccc taagcgtaaa atggcattga ttttccgttg gtacttaggt 32100

ctttctagtc gctggtcaaa ctcaggcgaa gtgggtcgtg aaatggatta tcaaatttgg 32160

gctggccctg ctctcggtgc atttaaccaa tgggcaaaag gcagttactt agataactat 32220

caagaccgaa atgccgtcga tttggcaaag cacttaatgt acggcgcggc ttacttaaat 32280

cgtattaact cgctaacggc tcaaggcgtt aaagtgccag cacagttact tcgctggaag 32340

ccaaaccaaa gaatggccta atacacttac aaagcaccag tctaaaaagc cactaatctt 32400

gattagtggc tttttttatt gtggtcaata tgaggctatt tagcctgtaa gcctgaaaat 32460

atcagcactc tgactttaca agcaaattat aattaaggca gggctctact catttatact 32520

gctagcaaac aagcaagttg cccagtaaaa caacaaggta cctgatttat atcgtcataa 32580

aagttggcta gagattcgtt attgatcttt actgattaga gtcgctctgt ttggaaaaag 32640

gtttctcgtt atcatcaaaa tacactctca aacctttaat caattacaac ttaggctttc 32700

tgcgggcatt tttatcttat ttgccacagc tgtatttgcc tttaggtttt gggtgcaact 32760

accattaatt gaggcctcat tagttaaatt atctgagcaa gagctcacct ctttaaatta 32820

cgcttttcag caaatgagaa agccactaca aaccattaat tacgactatg cggtgtggga 32880

cagaacctac agctatatga aatcaaactc agcgagcgct aaaaggtact atgaaaaaca 32940

tgagtaccca gatgatacgt tcaagagttt aaaagtcgac ggagtattta tattcaaccg 33000

tacaaatcag ccagttttta gtaaaggttt taatcataga aatgatatac cgctggtctt 33060

tgaattaact gactttaaac aacatccaca aaacatcgca ttatctccac aaaccaaaca 33120

ggcacaccca ccggcaagta agccgttaga ctcccctgat gatgtgcctt ctacccatgg 33180

ggttatcgcc acacgatacg gtccagcaat ttatagctct accagcattt taaaatctga 33240

tcgtagcggc tcccaacttg gttatttagt cttcattagg ttaattgatg aatggttcat 33300

cgctgagcta tcgcaataca ctgccgcagg tgttgaaatc gctatggctg atgccgcaga 33360

cgcacaatta gcgagattag gcgcaaacac taagcttaat aaagtaaccg ctacatccga 33420

acggttaata actaatgtcg atggtaagcc tctgttgaag ttagtgcttt accataccaa 33480

taaccaaccg ccgccgatgc tagattacag tataataatt ctattagttg agatgtcatt 33540

tttactgatc ctcgcttatt tcctttactc ctacttctta gtcaggccag ttagaaagct 33600

ggcttcagat attaaaaaaa tggataaaag tcgtgaaatt aaaaagctaa ggtatcacta 33660

ccctattact gagctagtca aagttgcgac tcacttcaac gccctaatgg ggacgattca 33720

ggaacaaact aaacagctta atgaacaagt ttttattgat aaattaacca atattcccaa 33780

tcgtcgcgct tttgagcagc gacttgaaac ctattgccaa ctgctagccc ggcaacaaat 33840

tggctttact ctcatcattg ccgatgtgga tcattttaaa gagtacaacg atactcttgg 33900

gcaccttgct ggggatgaag cattaataaa agtggcacaa acactatcgc aacagtttta 33960

ccgtgcagaa gatatttgtg cccgttttgg tggtgaagaa tttattatgt tatttcgaga 34020

catacctgat gagcccttgc agagaaagct cgatgcgatg ctgcactctt ttgcagagct 34080

caacctacct catccaaact catcaaccgc taattacgtt actgtgagcc ttggggtttg 34140

cacagttgtt gctgttgatg attttgaatt taaaagtgag tcgcatatta ttggcagtca 34200

ggctgcatta atcgcagata aggcgcttta tcatgctaaa gcctgtggtc gtaaccagtt 34260

gtcaaaaact actattactg ttgatgagat tgagcaatta gaagcaaata aaatcggtca 34320

tcaagcctaa actcgttcga gtactttccc ctaagtcaga gctatttgcc acttcaagat 34380

gtggctacaa ggcttactct ttcaaaacct gcatcaatag aacacagcaa aatacaataa 34440

tttaagtcaa tttagcctat taaacagagt taatgacagc tcatggtcgc aacttattag 34500

ctatttctag caatataaaa acttatccat tagtagtaac caataaaaaa actaatatat 34560

aaaactattt aatcattatt ttacagatga ttagctacca cccaccttaa gctggctata 34620

ttcgcactag taaaaataaa cattagatcg ggttcagatc aatttacgag tctcgtataa 34680

aatgtacaat aattcactta atttaatact gcatattttt acaagtagag agcggtgatg 34740

aaacaaaata cgaaaggctt tacattaatt gaattagtca tcgtgattat tattctcggt 34800

atacttgctg ctgtggcact gccgaaattc atcaatgttc aagatgacgc taggatctct 34860

gcgatgagcg gtcagttttc atcatttgaa agtgccgtaa aactatacca tagcggttgg 34920

ttagccaaag gctacaacac tgcggttgaa aagctctcag gctttggcca aggtaatgtt 34980

gcatcaagtg acacaggttt tccgtactca acatcaggca cgagtactga tgtgcataaa 35040

gcttgtggtg aactatggca tggcattacc gatacagact tcacaattgg tgcggttagt 35100

gatggcgatc taatgactgc agatgtcgat attgcttaca cctatcgtgg tgatatgtgt 35160

atctatcgcg atctgtattt tattcagcgc tcattaccta ctaaggtgat gaactacaaa 35220

tttaaaactg gtgaaataga aattattgat gctttctaca accctgacgg ctcaactggt 35280

caattaccat aaatttggcg cttatctaag ttgtacttgc tctgaccgac acaaataatg 35340

tcgtttctca gcatatatca aaatacacag caaaaatttg gggttagcta tatagctaac 35400

cccaaatcat atctaacttt acactgcatc taattccaaa cagtatccag ccaaaagcct 35460

aaactattgt tgactcagcg ctaaaatatg cgatgcaaca aacaagtctt ggatcgcaat 35520

acctgagcta tcaaaaatgg tcacctcatc agcactttga cgtcctgttg cggactcgtt 35580

tatcacctga ccaatctcaa ttatcggcgt atttctgcta tgttgaaact caccaataac 35640

aatagattga gaagcaaagt cgcaaaacaa gcgagcatga ctatataggt cagttggcaa 35700

ctcttgctta cccactttat cagcgcccat tgcagaaata tgcgttcctg cttgtaccca 35760

ctgcgcttca aataaaggcg cttgagctgt ggttgctgtg ataataatat ctgcttgttc 35820

acaagcagct tgtgcatcac aagcttcggc attaatgcct ttttctaata aacgcttaac 35880

caagttttca gttttgctag cactacggcc aactaccaat accttagtta atgaacgaac 35940

cttgctcact gctagcactt catattcagc ctgatgaccg gtaccaaaaa cagttaatac 36000

cgtagcatct tctctcgcga ggtaactcac tgctactgca tcggcagcac cagtgcggta 36060

agcattaacg gtagtggcag caatcaccgn ctgcaacata ccggttaatg gatcgagtaa 36120

aaatacgtta gtgccgtggc atggtaaacc atgtttatgg ttatcaggcc aatagctgcc 36180

tgttttccag ccgacaaggt ttggcgttga agccgacttt aatgagaaca tttcattaag 36240

gttcgcgccc tgtgcattaa ctaccgggaa caaggttgct ttatcatcta cggcagcgac 36300

aaacgcttct ttaacagcga tataagccag ctcatgggag atgagctttg atgtttgcgc 36360

ttcagttaaa tagatcatat taccacccct gcactcgatt ccagatctca tagccaccat 36420

tatcaccatc agtatcaaat acatggtact gagcgtgcat tgaagctgtt gcacaggcgt 36480

ggttcggcaa aatatgtaga cgactaccta ccgggaactg cgctaaatca ataacgccgc 36540

catcaactgc ttcaataatg ccgtgctctt gattaacagt tataacctgt agacctgata 36600

acacgtgacc gctgtcgtca cacactaaac cataaccaca atcttttggc tgctctgcag 36660

tacctctatc acccgaaaga gccatccaac ccgcatcaat gaaaatccag tttttatcag 36720

gattatgacc aataacactg gtcactaccg ttgcggcaat atcagttaac tgacacacgt 36780

ttagccctgc catgactaaa tcgaagaagg tgtacacacc cgctctaacc tcggtgatcc 36840

catcaaggtt ttgatagctt tgcgctgttg gtgttgaacc aatactaacg atgtcacatt 36900

gcatacccgc tgcgcgaatg cgtcagcagc ttgtacagcc gctgcaactt cattttgcgc 36960

cgcatcaatt aattgctgtt tttcaaaaca ttgatatgac tcaccagcgt gagtnagtac 37020

gccgtgaaaa ctcgctgcgc cagacgttag tatctgagca atttcaatca acttatcggc 37080

ttccggtgga ataccaccac gatggccatc acaatcaatt tcaattaatg ctggtatttg 37140

gcagtcataa gaaccacaga aatgatttag ctgatgcgct tgctcaacac tatcaagtaa 37200

aactcttgca ttaatacctt ggtccaacat tttagcaata cgcggcaact taccatcggc 37260

aatacctact gcataaataa tgtctgtgta acctttagat gctaaggcct cggcctcttt 37320

taccgttgat acagtgactg gtgagttttt agtgggtaat aaaaactcgg ctgcttcaag 37380

tgatcttaac gttttaaaat gcggtcttag gtttgcacct aatccttcaa ttttttggcg 37440

tagttgactg aggttattaa taaatactgg cttatttaca tataaaaacg gtgtatcaat 37500

tgcttgatac tgactttgct gagtcgtgga aagtatttga gtagatggca tctttaatat 37560

cctagttcat caatcaatct aacaagtttg atgcctagcc acagtggctt gtattcatga 37620

tgctttggaa aatgcttata ttcaaagtat ttgaaagaca tcaaacttct tgtttaatgc 37680

tcagtatcca ccagcacgca tttattttat attaactatt atcaagatat agattaggtt 37740

caaaccaaat gattagtact gaagatctac gttttatcag cgtaatcgcc agtcatcgca 37800

ccttagctga tgccgctaga acactaaata tcacgccacc atcagtgaca ttaaggttgc 37860

agcatattga aaagaaacta tcgattagcc tgatc 37895

2

654

Shewanella putrefaciens

2

Met Lys Gln Thr Leu Met Ala Ile Ser Ile Met Ser Leu Phe Ser Phe

1 5 10 15

Asn Ala Leu Ala Ala Gln His Glu His Asp His Ile Thr Val Asp Tyr

20 25 30

Glu Gly Lys Ala Ala Thr Glu His Thr Ile Ala His Asn Gln Ala Val

35 40 45

Ala Lys Thr Leu Asn Phe Ala Asp Thr Arg Ala Phe Glu Gln Ser Ser

50 55 60

Lys Asn Leu Val Ala Lys Phe Asp Lys Ala Thr Ala Asp Ile Leu Arg

65 70 75 80

Ala Glu Phe Ala Phe Ile Ser Asp Glu Ile Pro Asp Ser Val Asn Pro

85 90 95

Ser Leu Tyr Arg Gln Ala Gln Leu Asn Met Val Pro Asn Gly Tyr Lys

100 105 110

Val Ser Asp Gly Ile Tyr Gln Val Arg Gly Thr Asp Leu Ser Asn Leu

115 120 125

Thr Leu Ile Arg Ser Asp Asn Gly Trp Ile Ala Tyr Asp Val Leu Leu

130 135 140

Thr Lys Glu Ala Ala Lys Ala Ser Leu Gln Phe Ala Leu Lys Asn Leu

145 150 155 160

Pro Lys Asp Gly Asp Pro Val Val Ala Met Ile Tyr Ser His Ser His

165 170 175

Ala Asp His Phe Gly Gly Ala Arg Gly Val Gln Glu Met Phe Pro Asp

180 185 190

Val Lys Val Tyr Gly Ser Asp Asn Ile Thr Lys Glu Ile Val Asp Glu

195 200 205

Asn Val Leu Ala Gly Asn Ala Met Ser Arg Arg Ala Ala Tyr Gln Tyr

210 215 220

Gly Ala Thr Leu Gly Lys His Asp His Gly Ile Val Asp Ala Ala Leu

225 230 235 240

Gly Lys Gly Leu Ser Lys Gly Glu Ile Thr Tyr Val Ala Pro Asp Tyr

245 250 255

Thr Leu Asn Ser Glu Gly Lys Trp Glu Thr Leu Thr Ile Asp Gly Leu

260 265 270

Glu Met Val Phe Met Asp Ala Ser Gly Thr Glu Ala Glu Ser Glu Met

275 280 285

Ile Thr Tyr Ile Pro Ser Lys Lys Ala Leu Trp Thr Ala Glu Leu Thr

290 295 300

Tyr Gln Gly Met His Asn Ile Tyr Thr Leu Arg Gly Ala Lys Val Arg

305 310 315 320

Asp Ala Leu Lys Trp Ser Lys Asp Ile Asn Glu Met Ile Asn Ala Phe

325 330 335

Gly Gln Asp Val Glu Val Leu Phe Ala Ser His Ser Ala Pro Val Trp

340 345 350

Gly Asn Gln Ala Ile Asn Asp Phe Leu Arg Leu Gln Arg Asp Asn Tyr

355 360 365

Gly Leu Val His Asn Gln Thr Leu Arg Leu Ala Asn Asp Gly Val Gly

370 375 380

Ile Gln Asp Ile Gly Asp Ala Ile Gln Asp Thr Ile Pro Glu Ser Ile

385 390 395 400

Tyr Lys Thr Trp His Thr Asn Gly Tyr His Gly Thr Tyr Ser His Asn

405 410 415

Ala Lys Ala Val Tyr Asn Lys Tyr Leu Gly Tyr Phe Asp Met Asn Pro

420 425 430

Ala Asn Leu Asn Pro Leu Pro Thr Lys Gln Glu Ser Ala Lys Phe Val

435 440 445

Glu Tyr Met Gly Gly Ala Asp Ala Ala Ile Lys Arg Ala Lys Asp Asp

450 455 460

Tyr Ala Gln Gly Glu Tyr Arg Phe Val Ala Thr Ala Leu Asn Lys Val

465 470 475 480

Val Met Ala Glu Pro Glu Asn Asp Ser Ala Arg Gln Leu Leu Ala Asp

485 490 495

Thr Tyr Glu Gln Leu Gly Tyr Gln Ala Glu Gly Ala Gly Trp Arg Asn

500 505 510

Ile Tyr Leu Thr Gly Ala Gln Glu Leu Arg Val Gly Ile Gln Ala Gly

515 520 525

Ala Pro Lys Thr Ala Ser Ala Asp Val Ile Ser Glu Met Asp Met Pro

530 535 540

Thr Leu Phe Asp Phe Leu Ala Val Lys Ile Asp Ser Gln Gln Ala Ala

545 550 555 560

Lys His Gly Leu Val Lys Met Asn Val Ile Thr Pro Asp Thr Lys Asp

565 570 575

Ile Leu Tyr Ile Glu Leu Ser Asn Gly Asn Leu Ser Asn Ala Val Val

580 585 590

Asp Lys Glu Gln Leu Met Val Asn Lys Ala Asp Val Asn Arg Ile Leu

595 600 605

Leu Gly Gln Val Thr Leu Lys Ala Leu Leu Ala Ser Gly Asp Ala Lys

610 615 620

Leu Thr Gly Asp Lys Thr Ala Phe Ser Lys Ile Ala Asp Ser Met Val

625 630 635 640

Glu Phe Thr Pro Asp Phe Glu Ile Val Pro Thr Pro Val Lys

645 650

3

277

Shewanella putrefaciens

3

Ser Thr Lys Ala Ser Ala Arg Val Val Ala Lys Phe Asn Val Glu Glu

1 5 10 15

Ala Ala Ile Ser Ile Gln Gln Cys Gln Gly Ile Ser Leu Ala Phe Arg

20 25 30

Tyr Ser Asp Asp Leu His Gly Leu Leu Cys His Trp Asn Asp Ala Ala

35 40 45

Asn Met Gln Gln Glu Lys Ala Glu Ile Leu Gly Leu Gly Ser Lys Gln

50 55 60

Pro Glu Ala Asn Pro Lys Asn Ser Ser Ser Glu Leu Leu Ala Leu Gly

65 70 75 80

Ile Asp Gln Lys Leu Leu Val Gln Arg Gln Asn Leu Gln His Glu Val

85 90 95

Lys His Asp Ala Ile Ala Asp Ser Ile Asp Val Cys His Ser Leu Ser

100 105 110

Lys Pro Ala Asn Val Gly Leu Phe Thr Glu Ser Leu Ala Ser Phe Asp

115 120 125

Phe Ala Phe Ser Lys Leu Ser Leu Ala Leu Gly Leu Gly Lys Ala Lys

130 135 140

Ile Tyr Ser Glu Lys Leu Ala Trp Leu Asp Phe Phe Arg Asp Arg Gln

145 150 155 160

Leu Ala Glu Pro Leu Ala Leu Leu Ala Arg Lys Glu Ser Glu Ser Phe

165 170 175

Tyr His Ser Leu Ile Ser His Ile Asn Thr Ser Asn Arg Cys Arg Glu

180 185 190

Ile Asp Val Gly Phe Glu Ile Ser Ala Ser Asp Thr Glu Glu Lys Ser

195 200 205

Ala Gln Ser Ala Gly Lys Asn Asp Ala Thr Cys Ile Gly Val Leu Leu

210 215 220

Trp Asp Gly Ser His Ser Val Asn Phe His Val Gly Thr Gln Ala Phe

225 230 235 240

Gln Ala Asp Ser Leu Arg Pro Lys Gly Lys Asp Gly Tyr Glu Phe Arg

245 250 255

Trp Glu Asn Pro Arg Ile Glu Ser His Gln Ser Leu Leu Ala Arg Leu

260 265 270

Tyr Gly Arg Val Met

275

4

1480

Shewanella putrefaciens

4

gctagtctta gctgasrthr ysaasragct cgaacaacag ctttaaaatt cacttcttct 60

gctgcaatac ttatttgctg acactgacca atactcagtg caaaacgata actatcatca 120

agatggaaar gvavaaaysh asnvaggaaa asrgngncys gngysraaha rgtyrsrasa 180

shscccagta aacaatgcca attatcagca gcgttcattt gctgttcttt agcctcaatc 240

aaacctaaac cagacttttg tggctcagcg ttaggcttat taggycyshs trasnasaaa 300

aasnmtgngn gysaaggygy srysgnrgaa asnrysasns raactcgact ctagtaaagc 360

aagaccaata tcttgtttta acaaaacctg tcgctgatta agttgatgct caaccttgtg 420

atccgcaata gcatcggaaa tsrsrgaagy asgnysvagn arggnasngn hsgvayshsa 480

saaaaassra tcaacacaat ggctcaagct tttaggtgca ttaactccaa gaaaagtttc 540

gctcagtgca gagaagtcaa acgcaaaaga ttttagcgat aatgccagca svacyshssr 600

srysraaasn vagyhthrgs raasrhasha ahsryssraa ccaagtcctt tcgctttaat 660

gtaagactcc ttgagcgccc acaaatcaaa aaagcggtct cgctgcaagg cctctggtaa 720

cgctaacaag gctcgctttt gygyysaays tyrsrgysaa trashharga sarggnaagr 780

aaaaargysg ctgattcaga gaaataatga ctaagaatag agtggatatt ggtgctgtta 840

cggcaacgct caatgtcgac gccaaactca atactagcag agtcagtttc srgsrhtyrh 900

ssrsrhsasn thrsrasnar gcysarggas vagyhgsraa srasthrgct ccttgcttgc 960

ctgactggcg cctttattat cagcagtgca aatgcctact aatagccaat ctccactatg 1020

actcacatta aagtggaccc cggtttgagy ssraagnsra agyysasnas aathrcysgy 1080

vatrasgysr hssrvaasnh hsvagythrg ngcaaattgc gcatcactca atctaggctt 1140

acctttgtcg ccatattcaa agcgccattc attggggcgt atttcactat gttgtgacaa 1200

taaagcgcgc aaahgnaaas srargrysgy ysasgytyrg hargtrgasn rarggsrhsg 1260

nsraaargaa tagcctctta ccattaaacc ttgagtttta gcttcttgtt taatgtagcg 1320

attaacctta attaactcat cttcaggcag ccatgactta accaactcty rgyargvamt 1380

gygnthrysa aggnystyra rgasnvaysg asgrtrsrys vagtgtagtc tggttatcgc 1440

actcttgtat tgttaacgga cagaagtata aggaaatcaa 1480

5

970

Shewanella putrefaciens

5

Met Ser Met Phe Leu Asn Ser Lys Leu Ser Arg Ser Val Lys Leu Ala

1 5 10 15

Ile Ser Ala Gly Leu Thr Ala Ser Leu Ala Met Pro Val Phe Ala Glu

20 25 30

Glu Thr Ala Ala Glu Glu Gln Ile Glu Arg Val Ala Val Thr Gly Ser

35 40 45

Arg Ile Ala Lys Ala Glu Leu Thr Gln Pro Ala Pro Val Val Ser Leu

50 55 60

Ser Ala Glu Glu Leu Thr Lys Phe Gly Asn Gln Asp Leu Gly Ser Val

65 70 75 80

Leu Ala Glu Leu Pro Ala Ile Gly Ala Thr Asn Thr Ile Ile Gly Asn

85 90 95

Asn Asn Ser Asn Ser Ser Ala Gly Val Ser Ser Ala Asp Leu Arg Arg

100 105 110

Leu Gly Ala Asn Arg Thr Leu Val Leu Val Asn Gly Lys Arg Tyr Val

115 120 125

Ala Gly Gln Pro Gly Ser Ala Glu Val Asp Leu Ser Thr Ile Pro Thr

130 135 140

Ser Met Ile Ser Arg Val Glu Ile Val Thr Gly Gly Ala Ser Ala Ile

145 150 155 160

Tyr Gly Ser Asp Ala Val Ser Gly Val Ile Asn Val Ile Leu Lys Glu

165 170 175

Asp Phe Glu Gly Phe Glu Phe Asn Ala Arg Thr Ser Gly Ser Thr Glu

180 185 190

Ser Val Gly Thr Gln Glu His Ser Phe Asp Ile Leu Gly Gly Ala Asn

195 200 205

Val Ala Asp Gly Arg Gly Asn Val Thr Phe Tyr Ala Gly Tyr Glu Arg

210 215 220

Thr Lys Glu Val Met Ala Thr Asp Ile Arg Gln Phe Asp Ala Trp Gly

225 230 235 240

Thr Ile Lys Asn Glu Ala Asp Gly Gly Glu Asp Asp Gly Ile Pro Asp

245 250 255

Arg Leu Arg Val Pro Arg Val Tyr Ser Glu Met Ile Asn Ala Thr Gly

260 265 270

Val Ile Asn Ala Phe Gly Gly Gly Ile Gly Arg Ser Thr Phe Asp Ser

275 280 285

Asn Gly Asn Pro Ile Ala Gln Gln Glu Arg Asp Gly Thr Asn Ser Phe

290 295 300

Ala Phe Gly Ser Phe Pro Asn Gly Cys Asp Thr Cys Phe Asn Thr Glu

305 310 315 320

Ala Tyr Glu Asn Tyr Ile Pro Gly Val Glu Arg Ile Asn Val Gly Ser

325 330 335

Ser Phe Asn Phe Asp Phe Thr Asp Asn Ile Gln Phe Tyr Thr Asp Phe

340 345 350

Arg Tyr Val Lys Ser Asp Ile Gln Gln Gln Phe Gln Pro Ser Phe Arg

355 360 365

Phe Gly Asn Ile Asn Ile Asn Val Glu Asp Asn Ala Phe Leu Asn Asp

370 375 380

Asp Leu Arg Gln Gln Met Leu Asp Ala Gly Gln Thr Asn Ala Ser Phe

385 390 395 400

Ala Lys Phe Phe Asp Glu Leu Gly Asn Arg Ser Ala Glu Asn Lys Arg

405 410 415

Glu Leu Phe Arg Tyr Val Gly Gly Phe Lys Gly Gly Phe Asp Ile Ser

420 425 430

Glu Thr Ile Phe Asp Tyr Asp Leu Tyr Tyr Val Tyr Gly Glu Thr Asn

435 440 445

Asn Arg Arg Lys Thr Leu Asn Asp Leu Ile Pro Asp Asn Phe Val Ala

450 455 460

Ala Val Asp Ser Val Ile Asp Pro Asp Thr Gly Leu Ala Ala Cys Arg

465 470 475 480

Ser Gln Val Ala Ser Ala Gln Gly Asp Asp Tyr Thr Asp Pro Ala Ser

485 490 495

Val Asn Gly Ser Asp Cys Val Ala Tyr Asn Pro Phe Gly Met Gly Gln

500 505 510

Ala Ser Ala Glu Ala Arg Asp Trp Val Ser Ala Asp Val Thr Arg Glu

515 520 525

Asp Lys Ile Thr Gln Gln Val Ile Gly Gly Thr Leu Gly Thr Asp Ser

530 535 540

Glu Glu Leu Phe Glu Leu Gln Gly Gly Ala Ile Ala Met Val Val Gly

545 550 555 560

Phe Glu Tyr Arg Glu Glu Thr Ser Gly Ser Thr Thr Asp Glu Phe Thr

565 570 575

Lys Ala Gly Phe Leu Thr Ser Ala Ala Thr Pro Asp Ser Tyr Gly Glu

580 585 590

Tyr Asp Val Thr Glu Tyr Phe Val Glu Val Asn Ile Pro Val Leu Lys

595 600 605

Glu Leu Pro Phe Ala His Glu Leu Ser Phe Asp Gly Ala Tyr Arg Asn

610 615 620

Ala Asp Tyr Ser His Ala Gly Lys Thr Glu Ala Trp Lys Ala Gly Met

625 630 635 640

Phe Tyr Ser Pro Leu Glu Gln Leu Ala Leu Arg Gly Thr Val Gly Glu

645 650 655

Ala Val Arg Ala Pro Asn Ile Ala Glu Ala Phe Ser Pro Arg Ser Pro

660 665 670

Gly Phe Gly Arg Val Ser Asp Pro Cys Asp Ala Asp Asn Ile Asn Asp

675 680 685

Asp Pro Asp Arg Val Ser Asn Cys Ala Ala Leu Gly Ile Pro Pro Gly

690 695 700

Phe Gln Ala Asn Asp Asn Val Ser Val Asp Thr Leu Ser Gly Gly Asn

705 710 715 720

Pro Asp Leu Lys Pro Glu Thr Ser Thr Ser Phe Thr Gly Gly Leu Val

725 730 735

Trp Thr Pro Thr Phe Ala Asp Asn Leu Ser Phe Thr Val Asp Tyr Tyr

740 745 750

Asp Ile Gln Ile Glu Asp Ala Ile Leu Ser Val Ala Thr Gln Thr Val

755 760 765

Ala Asp Asn Cys Val Asp Ser Thr Gly Gly Pro Asp Thr Asp Phe Cys

770 775 780

Ser Gln Val Asp Arg Asn Pro Thr Thr Tyr Asp Ile Glu Leu Val Arg

785 790 795 800

Ser Gly Tyr Leu Asn Ala Ala Ala Leu Asn Thr Lys Gly Ile Glu Phe

805 810 815

Gln Ala Ala Tyr Ser Leu Asp Leu Glu Ser Phe Asn Ala Pro Gly Glu

820 825 830

Leu Arg Phe Asn Leu Leu Gly Asn Gln Leu Leu Glu Leu Glu Arg Leu

835 840 845

Glu Phe Gln Asn Arg Pro Asp Glu Ile Asn Asp Glu Lys Gly Glu Val

850 855 860

Gly Asp Pro Glu Leu Gln Phe Arg Leu Gly Ile Asp Tyr Arg Leu Asp

865 870 875 880

Asp Leu Ser Val Ser Trp Asn Thr Arg Tyr Ile Asp Ser Val Val Thr

885 890 895

Tyr Asp Val Ser Glu Asn Gly Gly Ser Pro Glu Asp Leu Tyr Pro Gly

900 905 910

His Ile Gly Ser Met Thr Thr His Asp Leu Ser Ala Thr Tyr Tyr Ile

915 920 925

Asn Glu Asn Phe Met Ile Asn Gly Gly Val Arg Asn Leu Phe Asp Ala

930 935 940

Leu Pro Pro Gly Tyr Thr Asn Asp Ala Leu Tyr Asp Leu Val Gly Arg

945 950 955 960

Arg Ala Phe Leu Gly Ile Lys Val Met Met

965 970

6

288

Shewanella putrefaciens

6

Met Ala Lys Ile Asn Ser Glu His Leu Asp Glu Ala Thr Ile Thr Ser

1 5 10 15

Asn Lys Cys Thr Gln Thr Glu Thr Glu Ala Arg His Arg Asn Ala Thr

20 25 30

Thr Thr Pro Glu Met Arg Arg Phe Ile Gln Glu Ser Asp Leu Ser Val

35 40 45

Ser Gln Leu Ser Lys Ile Leu Asn Ile Ser Glu Ala Thr Val Arg Lys

50 55 60

Trp Arg Lys Arg Asp Ser Val Glu Asn Cys Pro Asn Thr Pro His His

65 70 75 80

Leu Asn Thr Thr Leu Thr Pro Leu Gln Glu Tyr Val Val Val Gly Leu

85 90 95

Arg Tyr Gln Leu Lys Met Pro Leu Asp Arg Leu Leu Lys Ala Thr Gln

100 105 110

Glu Phe Ile Asn Pro Asn Val Ser Arg Ser Gly Leu Ala Arg Cys Leu

115 120 125

Lys Arg Tyr Gly Val Ser Arg Val Ser Asp Ile Gln Ser Pro His Val

130 135 140

Pro Met Arg Tyr Phe Asn Gln Ile Pro Val Thr Gln Gly Ser Asp Val

145 150 155 160

Gln Thr Tyr Thr Leu His Tyr Glu Thr Leu Ala Lys Thr Leu Ala Leu

165 170 175

Pro Ser Thr Asp Gly Asp Asn Val Val Gln Val Val Ser Leu Thr Ile

180 185 190

Pro Pro Lys Leu Thr Glu Glu Ala Pro Ser Ser Ile Leu Leu Gly Ile

195 200 205

Asp Pro His Ser Asp Trp Ile Tyr Leu Asp Ile Tyr Gln Asp Gly Asn

210 215 220

Thr Gln Ala Thr Asn Arg Tyr Met Ala Tyr Val Leu Lys His Gly Pro

225 230 235 240

Phe His Leu Arg Lys Leu Leu Val Arg Asn Tyr His Thr Phe Leu Gln

245 250 255

Arg Phe Pro Gly Ala Thr Gln Asn Arg Arg Pro Ser Lys Asp Met Pro

260 265 270

Glu Thr Ile Asn Lys Thr Pro Glu Thr Gln Ala Pro Ser Gly Asp Ser

275 280 285

7

2756

Shewanella putrefaciens

7

Met Ser Gln Thr Ser Lys Pro Thr Asn Ser Ala Thr Glu Gln Ala Gln

1 5 10 15

Asp Ser Gln Ala Asp Ser Arg Leu Asn Lys Arg Leu Lys Asp Met Pro

20 25 30

Ile Ala Ile Val Gly Met Ala Ser Ile Phe Ala Asn Ser Arg Tyr Leu

35 40 45

Asn Lys Phe Trp Asp Leu Ile Ser Glu Lys Ile Asp Ala Ile Thr Glu

50 55 60

Leu Pro Ser Thr His Trp Gln Pro Glu Glu Tyr Tyr Asp Ala Asp Lys

65 70 75 80

Thr Ala Ala Asp Lys Ser Tyr Cys Lys Arg Gly Gly Phe Leu Pro Asp

85 90 95

Val Asp Phe Asn Pro Met Glu Phe Gly Leu Pro Pro Asn Ile Leu Glu

100 105 110

Leu Thr Asp Ser Ser Gln Leu Leu Ser Leu Ile Val Ala Lys Glu Val

115 120 125

Leu Ala Asp Ala Asn Leu Pro Glu Asn Tyr Asp Arg Asp Lys Ile Gly

130 135 140

Ile Thr Leu Gly Val Gly Gly Gly Gln Lys Ile Ser His Ser Leu Thr

145 150 155 160

Ala Arg Leu Gln Tyr Pro Val Leu Lys Lys Val Phe Ala Asn Ser Gly

165 170 175

Ile Ser Asp Thr Asp Ser Glu Met Leu Ile Lys Lys Phe Gln Asp Gln

180 185 190

Tyr Val His Trp Glu Glu Asn Ser Phe Pro Gly Ser Leu Gly Asn Val

195 200 205

Ile Ala Gly Arg Ile Ala Asn Arg Phe Asp Phe Gly Gly Met Asn Cys

210 215 220

Val Val Asp Ala Ala Cys Ala Gly Ser Leu Ala Ala Met Arg Met Ala

225 230 235 240

Leu Thr Glu Leu Thr Glu Gly Arg Ser Glu Met Met Ile Thr Gly Gly

245 250 255

Val Cys Thr Asp Asn Ser Pro Ser Met Tyr Met Ser Phe Ser Lys Thr

260 265 270

Pro Ala Phe Thr Thr Asn Glu Thr Ile Gln Pro Phe Asp Ile Asp Ser

275 280 285

Lys Gly Met Met Ile Gly Glu Gly Ile Gly Met Val Ala Leu Lys Arg

290 295 300

Leu Glu Asp Ala Glu Arg Asp Gly Asp Arg Ile Tyr Ser Val Ile Lys

305 310 315 320

Gly Val Gly Ala Ser Ser Asp Gly Lys Phe Lys Ser Ile Tyr Ala Pro

325 330 335

Arg Pro Ser Gly Gln Ala Lys Ala Leu Asn Arg Ala Tyr Asp Asp Ala

340 345 350

Gly Phe Ala Pro His Thr Leu Gly Leu Ile Glu Ala His Gly Thr Gly

355 360 365

Thr Ala Ala Gly Asp Ala Ala Glu Phe Ala Gly Leu Cys Ser Val Phe

370 375 380

Ala Glu Gly Asn Asp Thr Lys Gln His Ile Ala Leu Gly Ser Val Lys

385 390 395 400

Ser Gln Ile Gly His Thr Lys Ser Thr Ala Gly Thr Ala Gly Leu Ile

405 410 415

Lys Ala Ala Leu Ala Leu His His Lys Val Leu Pro Pro Thr Ile Asn

420 425 430

Val Ser Gln Pro Ser Pro Lys Leu Asp Ile Glu Asn Ser Pro Phe Tyr

435 440 445

Leu Asn Thr Glu Thr Arg Pro Trp Leu Pro Arg Val Asp Gly Thr Pro

450 455 460

Arg Arg Ala Gly Ile Ser Ser Phe Gly Phe Gly Gly Thr Asn Phe His

465 470 475 480

Phe Val Leu Glu Glu Tyr Asn Gln Glu His Ser Arg Thr Asp Ser Glu

485 490 495

Lys Ala Lys Tyr Arg Gln Arg Gln Val Ala Gln Ser Phe Leu Val Ser

500 505 510

Ala Ser Asp Lys Ala Ser Leu Ile Asn Glu Leu Asn Val Leu Ala Ala

515 520 525

Ser Ala Ser Gln Ala Glu Phe Ile Leu Lys Asp Ala Ala Ala Asn Tyr

530 535 540

Gly Val Arg Glu Leu Asp Lys Asn Ala Pro Arg Ile Gly Leu Val Ala

545 550 555 560

Asn Thr Ala Glu Glu Leu Ala Gly Leu Ile Lys Gln Ala Leu Ala Lys

565 570 575

Leu Ala Ala Ser Asp Asp Asn Ala Trp Gln Leu Pro Gly Gly Thr Ser

580 585 590

Tyr Arg Ala Ala Ala Val Glu Gly Lys Val Ala Ala Leu Phe Ala Gly

595 600 605

Gln Gly Ser Gln Tyr Leu Asn Met Gly Arg Asp Leu Thr Cys Tyr Tyr

610 615 620

Pro Glu Met Arg Gln Gln Phe Val Thr Ala Asp Lys Val Phe Ala Ala

625 630 635 640

Asn Asp Lys Thr Pro Leu Ser Gln Thr Leu Tyr Pro Lys Pro Val Phe

645 650 655

Asn Lys Asp Glu Leu Lys Ala Gln Glu Ala Ile Leu Thr Asn Thr Ala

660 665 670

Asn Ala Gln Ser Ala Ile Gly Ala Ile Ser Met Gly Gln Tyr Asp Leu

675 680 685

Phe Thr Ala Ala Gly Phe Asn Ala Asp Met Val Ala Gly His Ser Phe

690 695 700

Gly Glu Leu Ser Ala Leu Cys Ala Ala Gly Val Ile Ser Ala Asp Asp

705 710 715 720

Tyr Tyr Lys Leu Ala Phe Ala Arg Gly Glu Ala Met Ala Thr Lys Ala

725 730 735

Pro Ala Lys Asp Gly Val Glu Ala Asp Ala Gly Ala Met Phe Ala Ile

740 745 750

Ile Thr Lys Ser Ala Ala Asp Leu Glu Thr Val Glu Ala Thr Ile Ala

755 760 765

Lys Phe Asp Gly Val Lys Val Ala Asn Tyr Asn Ala Pro Thr Gln Ser

770 775 780

Val Ile Ala Gly Pro Thr Ala Thr Thr Ala Asp Ala Ala Lys Ala Leu

785 790 795 800

Thr Glu Leu Gly Tyr Lys Ala Ile Asn Leu Pro Val Ser Gly Ala Phe

805 810 815

His Thr Glu Leu Val Gly His Ala Gln Ala Pro Phe Ala Lys Ala Ile

820 825 830

Asp Ala Ala Lys Phe Thr Lys Thr Ser Arg Ala Leu Tyr Ser Asn Ala

835 840 845

Thr Gly Gly Leu Tyr Glu Ser Thr Ala Ala Lys Ile Lys Ala Ser Phe

850 855 860

Lys Lys His Met Leu Gln Ser Val Arg Phe Thr Ser Gln Leu Glu Ala

865 870 875 880

Met Tyr Asn Asp Gly Ala Arg Val Phe Val Glu Phe Gly Pro Lys Asn

885 890 895

Ile Leu Gln Lys Leu Val Gln Gly Thr Leu Val Asn Thr Glu Asn Glu

900 905 910

Val Cys Thr Ile Ser Ile Asn Pro Asn Pro Lys Val Asp Ser Asp Leu

915 920 925

Gln Leu Lys Gln Ala Ala Met Gln Leu Ala Val Thr Gly Val Val Leu

930 935 940

Ser Glu Ile Asp Pro Tyr Gln Ala Asp Ile Ala Ala Pro Ala Lys Lys

945 950 955 960

Ser Pro Met Ser Ile Ser Leu Asn Ala Ala Asn His Ile Ser Lys Ala

965 970 975

Thr Arg Ala Lys Met Ala Lys Ser Leu Glu Thr Gly Ile Val Thr Ser

980 985 990

Gln Ile Glu His Val Ile Glu Glu Lys Ile Val Glu Val Glu Lys Leu

995 1000 1005

Val Glu Val Glu Lys Ile Val Glu Lys Val Val Glu Val Glu Lys Val

1010 1015 1020

Val Glu Val Glu Ala Pro Val Asn Ser Val Gln Ala Asn Ala Ile Gln

1025 1030 1035 1040

Thr Arg Ser Val Val Ala Pro Val Ile Glu Asn Gln Val Val Ser Lys

1045 1050 1055

Asn Ser Lys Pro Ala Val Gln Ser Ile Ser Gly Asp Ala Leu Ser Asn

1060 1065 1070

Phe Phe Ala Ala Gln Gln Gln Thr Ala Gln Leu His Gln Gln Phe Leu

1075 1080 1085

Ala Ile Pro Gln Gln Tyr Gly Glu Thr Phe Thr Thr Leu Met Thr Glu

1090 1095 1100

Gln Ala Lys Leu Ala Ser Ser Gly Val Ala Ile Pro Glu Ser Leu Gln

1105 1110 1115 1120

Arg Ser Met Glu Gln Phe His Gln Leu Gln Ala Gln Thr Leu Gln Ser

1125 1130 1135

His Thr Gln Phe Leu Glu Met Gln Ala Gly Ser Asn Ile Ala Ala Leu

1140 1145 1150

Asn Leu Leu Asn Ser Ser Gln Ala Thr Tyr Ala Pro Ala Ile His Asn

1155 1160 1165

Glu Ala Ile Gln Ser Gln Val Val Gln Ser Gln Thr Ala Val Gln Pro

1170 1175 1180

Val Ile Ser Thr Gln Val Asn His Val Ser Glu Gln Pro Thr Gln Ala

1185 1190 1195 1200

Pro Ala Pro Lys Ala Gln Pro Ala Pro Val Thr Thr Ala Val Gln Thr

1205 1210 1215

Ala Pro Ala Gln Val Val Arg Gln Ala Ala Pro Val Gln Ala Ala Ile

1220 1225 1230

Glu Pro Ile Asn Thr Ser Val Ala Thr Thr Thr Pro Ser Ala Phe Ser

1235 1240 1245

Ala Glu Thr Ala Leu Ser Ala Thr Lys Val Gln Ala Thr Met Leu Glu

1250 1255 1260

Val Val Ala Glu Lys Thr Gly Tyr Pro Thr Glu Met Leu Glu Leu Glu

1265 1270 1275 1280

Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile Lys Arg Val Glu

1285 1290 1295

Ile Leu Gly Thr Val Gln Asp Glu Leu Pro Gly Leu Pro Glu Leu Ser

1300 1305 1310

Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly Glu Ile Val Asp Tyr

1315 1320 1325

Met Gly Ser Lys Leu Pro Ala Glu Gly Ser Met Asn Ser Gln Leu Ser

1330 1335 1340

Thr Gly Ser Ala Ala Ala Thr Pro Ala Ala Asn Gly Leu Ser Ala Glu

1345 1350 1355 1360

Lys Val Gln Ala Thr Met Met Ser Val Val Ala Glu Lys Thr Gly Tyr

1365 1370 1375

Pro Thr Glu Met Leu Glu Leu Glu Met Asp Met Glu Ala Asp Leu Gly

1380 1385 1390

Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu

1395 1400 1405

Leu Pro Gly Leu Pro Glu Leu Ser Pro Glu Asp Leu Ala Glu Cys Arg

1410 1415 1420

Thr Leu Gly Glu Ile Val Asp Tyr Met Asn Ser Lys Leu Ala Asp Gly

1425 1430 1435 1440

Ser Lys Leu Pro Ala Glu Gly Ser Met Asn Ser Gln Leu Ser Thr Ser

1445 1450 1455

Ala Ala Ala Ala Thr Pro Ala Ala Asn Gly Leu Ser Ala Glu Lys Val

1460 1465 1470

Gln Ala Thr Met Met Ser Val Val Ala Glu Lys Thr Gly Tyr Pro Thr

1475 1480 1485

Glu Met Leu Glu Leu Glu Met Asp Met Glu Ala Asp Leu Gly Ile Asp

1490 1495 1500

Ser Ile Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu Leu Pro

1505 1510 1515 1520

Gly Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu

1525 1530 1535

Gly Glu Ile Val Thr Tyr Met Asn Ser Lys Leu Ala Asp Gly Ser Lys

1540 1545 1550

Leu Pro Ala Glu Gly Ser Met His Tyr Gln Leu Ser Thr Ser Thr Ala

1555 1560 1565

Ala Ala Thr Pro Val Ala Asn Gly Leu Ser Ala Glu Lys Val Gln Ala

1570 1575 1580

Thr Met Met Ser Val Val Ala Asp Lys Thr Gly Tyr Pro Thr Glu Met

1585 1590 1595 1600

Leu Glu Leu Glu Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile

1605 1610 1615

Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu Leu Pro Gly Leu

1620 1625 1630

Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly Glu

1635 1640 1645

Ile Val Asp Tyr Met Gly Ser Lys Leu Pro Ala Glu Gly Ser Ala Asn

1650 1655 1660

Thr Ser Ala Ala Ala Ser Leu Asn Val Ser Ala Val Ala Ala Pro Gln

1665 1670 1675 1680

Ala Ala Ala Thr Pro Val Ser Asn Gly Leu Ser Ala Glu Lys Val Gln

1685 1690 1695

Ser Thr Met Met Ser Val Val Ala Glu Lys Thr Gly Tyr Pro Thr Glu

1700 1705 1710

Met Leu Glu Leu Gly Met Asp Met Glu Ala Asp Leu Gly Ile Asp Ser

1715 1720 1725

Ile Lys Arg Val Glu Ile Leu Gly Thr Val Gln Asp Glu Leu Pro Gly

1730 1735 1740

Leu Pro Glu Leu Asn Pro Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly

1745 1750 1755 1760

Glu Ile Val Asp Tyr Met Asn Ser Lys Leu Ala Asp Gly Ser Lys Leu

1765 1770 1775

Pro Ala Glu Gly Ser Ala Asn Thr Ser Ala Thr Ala Ala Thr Pro Ala

1780 1785 1790

Val Asn Gly Leu Ser Ala Asp Lys Val Gln Ala Thr Met Met Ser Val

1795 1800 1805

Val Ala Glu Lys Thr Gly Tyr Pro Thr Glu Met Leu Glu Leu Gly Met

1810 1815 1820

Asp Met Glu Ala Asp Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile

1825 1830 1835 1840

Leu Gly Thr Val Gln Asp Glu Leu Pro Gly Leu Pro Glu Leu Asn Pro

1845 1850 1855

Glu Asp Leu Ala Glu Cys Arg Thr Leu Gly Glu Ile Val Ser Tyr Met

1860 1865 1870

Asn Ser Gln Leu Ala Asp Gly Ser Lys Leu Ser Thr Ser Ala Ala Glu

1875 1880 1885

Gly Ser Ala Asp Thr Ser Ala Ala Asn Ala Ala Lys Pro Ala Ala Ile

1890 1895 1900

Ser Ala Glu Pro Ser Val Glu Leu Pro Pro His Ser Glu Val Ala Leu

1905 1910 1915 1920

Lys Lys Leu Asn Ala Ala Asn Lys Leu Glu Asn Cys Phe Ala Ala Asp

1925 1930 1935

Ala Ser Val Val Ile Asn Asp Asp Gly His Asn Ala Gly Val Leu Ala

1940 1945 1950

Glu Lys Leu Ile Lys Gln Gly Leu Lys Val Ala Val Val Arg Leu Pro

1955 1960 1965

Lys Gly Gln Pro Gln Ser Pro Leu Ser Ser Asp Val Ala Ser Phe Glu

1970 1975 1980

Leu Ala Ser Ser Gln Glu Ser Glu Leu Glu Ala Ser Ile Thr Ala Val

1985 1990 1995 2000

Ile Ala Gln Ile Glu Thr Gln Val Gly Ala Ile Gly Gly Phe Ile His

2005 2010 2015

Leu Gln Pro Glu Ala Asn Thr Glu Glu Gln Thr Ala Val Asn Leu Asp

2020 2025 2030

Ala Gln Ser Phe Thr His Val Ser Asn Ala Phe Leu Trp Ala Lys Leu

2035 2040 2045

Leu Gln Pro Lys Leu Val Ala Gly Ala Asp Ala Arg Arg Cys Phe Val

2050 2055 2060

Thr Val Ser Arg Ile Asp Gly Gly Phe Gly Tyr Leu Asn Thr Asp Ala

2065 2070 2075 2080

Leu Lys Asp Ala Glu Leu Asn Gln Ala Ala Leu Ala Gly Leu Thr Lys

2085 2090 2095

Thr Leu Ser His Glu Trp Pro Gln Val Phe Cys Arg Ala Leu Asp Ile

2100 2105 2110

Ala Thr Asp Val Asp Ala Thr His Leu Ala Asp Ala Ile Thr Ser Glu

2115 2120 2125

Leu Phe Asp Ser Gln Ala Gln Leu Pro Glu Val Gly Leu Ser Leu Ile

2130 2135 2140

Asp Gly Lys Val Asn Arg Val Thr Leu Val Ala Ala Glu Ala Ala Asp

2145 2150 2155 2160

Lys Thr Ala Lys Ala Glu Leu Asn Ser Thr Asp Lys Ile Leu Val Thr

2165 2170 2175

Gly Gly Ala Lys Gly Val Thr Phe Glu Cys Ala Leu Ala Leu Ala Ser

2180 2185 2190

Arg Ser Gln Ser His Phe Ile Leu Ala Gly Arg Ser Glu Leu Gln Ala

2195 2200 2205

Leu Pro Ser Trp Ala Glu Gly Lys Gln Thr Ser Glu Leu Lys Ser Ala

2210 2215 2220

Ala Ile Ala His Ile Ile Ser Thr Gly Gln Lys Pro Thr Pro Lys Gln

2225 2230 2235 2240

Val Glu Ala Ala Val Trp Pro Val Gln Ser Ser Ile Glu Ile Asn Ala

2245 2250 2255

Ala Leu Ala Ala Phe Asn Lys Val Gly Ala Ser Ala Glu Tyr Val Ser

2260 2265 2270

Met Asp Val Thr Asp Ser Ala Ala Ile Thr Ala Ala Leu Asn Gly Arg

2275 2280 2285

Ser Asn Glu Ile Thr Gly Leu Ile His Gly Ala Gly Val Leu Ala Asp

2290 2295 2300

Lys His Ile Gln Asp Lys Thr Leu Ala Glu Leu Ala Lys Val Tyr Gly

2305 2310 2315 2320

Thr Lys Val Asn Gly Leu Lys Ala Leu Leu Ala Ala Leu Glu Pro Ser

2325 2330 2335

Lys Ile Lys Leu Leu Ala Met Phe Ser Ser Ala Ala Gly Phe Tyr Gly

2340 2345 2350

Asn Ile Gly Gln Ser Asp Tyr Ala Met Ser Asn Asp Ile Leu Asn Lys

2355 2360 2365

Ala Ala Leu Gln Phe Thr Ala Arg Asn Pro Gln Ala Lys Val Met Ser

2370 2375 2380

Phe Asn Trp Gly Pro Trp Asp Gly Gly Met Val Asn Pro Ala Leu Lys

2385 2390 2395 2400

Lys Met Phe Thr Glu Arg Gly Val Tyr Val Ile Pro Leu Lys Ala Gly

2405 2410 2415

Ala Glu Leu Phe Ala Thr Gln Leu Leu Ala Glu Thr Gly Val Gln Leu

2420 2425 2430

Leu Ile Gly Thr Ser Met Gln Gly Gly Ser Asp Thr Lys Ala Thr Glu

2435 2440 2445

Thr Ala Ser Val Lys Lys Leu Asn Ala Gly Glu Val Leu Ser Ala Ser

2450 2455 2460

His Pro Arg Ala Gly Ala Gln Lys Thr Pro Leu Gln Ala Val Thr Ala

2465 2470 2475 2480

Thr Arg Leu Leu Thr Pro Ser Ala Met Val Phe Ile Glu Asp His Arg

2485 2490 2495

Ile Gly Gly Asn Ser Val Leu Pro Thr Val Cys Ala Ile Asp Trp Met

2500 2505 2510

Arg Glu Ala Ala Ser Asp Met Leu Gly Ala Gln Val Lys Val Leu Asp

2515 2520 2525

Tyr Lys Leu Leu Lys Gly Ile Val Phe Glu Thr Asp Glu Pro Gln Glu

2530 2535 2540

Leu Thr Leu Glu Leu Thr Pro Asp Asp Ser Asp Glu Ala Thr Leu Gln

2545 2550 2555 2560

Ala Leu Ile Ser Cys Asn Gly Arg Pro Gln Tyr Lys Ala Thr Leu Ile

2565 2570 2575

Ser Asp Asn Ala Asp Ile Lys Gln Leu Asn Lys Gln Phe Asp Leu Ser

2580 2585 2590

Ala Lys Ala Ile Thr Thr Ala Lys Glu Leu Tyr Ser Asn Gly Thr Leu

2595 2600 2605

Phe His Gly Pro Arg Leu Gln Gly Ile Gln Ser Val Val Gln Phe Asp

2610 2615 2620

Asp Gln Gly Leu Ile Ala Lys Val Ala Leu Pro Lys Val Glu Leu Ser

2625 2630 2635 2640

Asp Cys Gly Glu Phe Leu Pro Gln Thr His Met Gly Gly Ser Gln Pro

2645 2650 2655

Phe Ala Glu Asp Leu Leu Leu Gln Ala Met Leu Val Trp Ala Arg Leu

2660 2665 2670

Lys Thr Gly Ser Ala Ser Leu Pro Ser Ser Ile Gly Glu Phe Thr Ser

2675 2680 2685

Tyr Gln Pro Met Ala Phe Gly Glu Thr Gly Thr Ile Glu Leu Glu Val

2690 2695 2700

Ile Lys His Asn Lys Arg Ser Leu Glu Ala Asn Val Ala Leu Tyr Arg

2705 2710 2715 2720

Asp Asn Gly Glu Leu Ser Ala Met Phe Lys Ser Ala Lys Ile Thr Ile

2725 2730 2735

Ser Lys Ser Leu Asn Ser Ala Phe Leu Pro Ala Val Leu Ala Asn Asp

2740 2745 2750

Ser Glu Ala Asn

2755

8

771

Shewanella putrefaciens

8

Met Pro Leu Arg Ile Ala Leu Ile Leu Leu Pro Thr Pro Gln Phe Glu

1 5 10 15

Val Asn Ser Val Asp Gln Ser Val Leu Ala Ser Tyr Gln Thr Leu Gln

20 25 30

Pro Glu Leu Asn Ala Leu Leu Asn Ser Ala Pro Thr Pro Glu Met Leu

35 40 45

Ser Ile Thr Ile Ser Asp Asp Ser Asp Ala Asn Ser Phe Glu Ser Gln

50 55 60

Leu Asn Ala Ala Thr Asn Ala Ile Asn Asn Gly Tyr Ile Val Lys Leu

65 70 75 80

Ala Thr Ala Thr His Ala Leu Leu Met Leu Pro Ala Leu Lys Ala Ala

85 90 95

Gln Met Arg Ile His Pro His Ala Gln Leu Ala Ala Met Gln Gln Ala

100 105 110

Lys Ser Thr Pro Met Ser Gln Val Ser Gly Glu Leu Lys Leu Gly Ala

115 120 125

Asn Ala Leu Ser Leu Ala Gln Thr Asn Ala Leu Ser His Ala Leu Ser

130 135 140

Gln Ala Lys Arg Asn Leu Thr Asp Val Ser Val Asn Glu Cys Phe Glu

145 150 155 160

Asn Leu Lys Ser Glu Gln Gln Phe Thr Glu Val Tyr Ser Leu Ile Gln

165 170 175

Gln Leu Ala Ser Arg Thr His Val Arg Lys Glu Val Asn Gln Gly Val

180 185 190

Glu Leu Gly Pro Lys Gln Ala Lys Ser His Tyr Trp Phe Ser Glu Phe

195 200 205

His Gln Asn Arg Val Ala Ala Ile Asn Phe Ile Asn Gly Gln Gln Ala

210 215 220

Thr Ser Tyr Val Leu Thr Gln Gly Ser Gly Leu Leu Ala Ala Lys Ser

225 230 235 240

Met Leu Asn Gln Gln Arg Leu Met Phe Ile Leu Pro Gly Asn Ser Gln

245 250 255

Gln Gln Ile Thr Ala Ser Ile Thr Gln Leu Met Gln Gln Leu Glu Arg

260 265 270

Leu Gln Val Thr Glu Val Asn Glu Leu Ser Leu Glu Cys Gln Leu Glu

275 280 285

Leu Leu Ser Ile Met Tyr Asp Asn Leu Val Asn Ala Asp Lys Leu Thr

290 295 300

Thr Arg Asp Ser Lys Pro Ala Tyr Gln Ala Val Ile Gln Ala Ser Ser

305 310 315 320

Val Ser Ala Ala Lys Gln Glu Leu Ser Ala Leu Asn Asp Ala Leu Thr

325 330 335

Ala Leu Phe Ala Glu Gln Thr Asn Ala Thr Ser Thr Asn Lys Gly Leu

340 345 350

Ile Gln Tyr Lys Thr Pro Ala Gly Ser Tyr Leu Thr Leu Thr Pro Leu

355 360 365

Gly Ser Asn Asn Asp Asn Ala Gln Ala Gly Leu Ala Phe Val Tyr Pro

370 375 380

Gly Val Gly Thr Val Tyr Ala Asp Met Leu Asn Glu Leu His Gln Tyr

385 390 395 400

Phe Pro Ala Leu Tyr Ala Lys Leu Glu Arg Glu Gly Asp Leu Lys Ala

405 410 415

Met Leu Gln Ala Glu Asp Ile Tyr His Leu Asp Pro Lys His Ala Ala

420 425 430

Gln Met Ser Leu Gly Asp Leu Ala Ile Ala Gly Val Gly Ser Ser Tyr

435 440 445

Leu Leu Thr Gln Leu Leu Thr Asp Glu Phe Asn Ile Lys Pro Asn Phe

450 455 460

Ala Leu Gly Tyr Ser Met Gly Glu Ala Ser Met Trp Ala Ser Leu Gly

465 470 475 480

Val Trp Gln Asn Pro His Ala Leu Ile Ser Lys Thr Gln Thr Asp Pro

485 490 495

Leu Phe Thr Ser Ala Ile Ser Gly Lys Leu Thr Ala Val Arg Gln Ala

500 505 510

Trp Gln Leu Asp Asp Thr Ala Ala Glu Ile Gln Trp Asn Ser Phe Val

515 520 525

Val Arg Ser Glu Ala Ala Pro Ile Glu Ala Leu Leu Lys Asp Tyr Pro

530 535 540

His Ala Tyr Leu Ala Ile Ile Gln Gly Asp Thr Cys Val Ile Ala Gly

545 550 555 560

Cys Glu Ile Gln Cys Lys Ala Leu Leu Ala Ala Leu Gly Lys Arg Gly

565 570 575

Ile Ala Ala Asn Arg Val Thr Ala Met His Thr Gln Pro Ala Met Gln

580 585 590

Glu His Gln Asn Val Met Asp Phe Tyr Leu Gln Pro Leu Lys Ala Glu

595 600 605

Leu Pro Ser Glu Ile Ser Phe Ile Ser Ala Ala Asp Leu Thr Ala Lys

610 615 620

Gln Thr Val Ser Glu Gln Ala Leu Ser Ser Gln Val Val Ala Gln Ser

625 630 635 640

Ile Ala Asp Thr Phe Cys Gln Thr Leu Asp Phe Thr Ala Leu Val His

645 650 655

His Ala Gln His Gln Gly Ala Lys Leu Phe Val Glu Ile Gly Ala Asp

660 665 670

Arg Gln Asn Cys Thr Leu Ile Asp Lys Ile Val Lys Gln Asp Gly Ala

675 680 685

Ser Ser Val Gln His Gln Pro Cys Cys Thr Val Pro Met Asn Ala Lys

690 695 700

Gly Ser Gln Asp Ile Thr Ser Val Ile Lys Ala Leu Gly Gln Leu Ile

705 710 715 720

Ser His Gln Val Pro Leu Ser Val Gln Pro Phe Ile Asp Gly Leu Lys

725 730 735

Arg Glu Leu Thr Leu Cys Gln Leu Thr Ser Gln Gln Leu Ala Ala His

740 745 750

Ala Asn Val Asp Ser Lys Phe Glu Ser Asn Gln Asp His Leu Leu Gln

755 760 765

Gly Glu Val

770

9

2004

Shewanella putrefaciens

9

Met Ser Leu Pro Asp Asn Ala Ser Asn His Leu Ser Ala Asn Gln Lys

1 5 10 15

Gly Ala Ser Gln Ala Ser Lys Thr Ser Lys Gln Ser Lys Ile Ala Ile

20 25 30

Val Gly Leu Ala Thr Leu Tyr Pro Asp Ala Lys Thr Pro Gln Glu Phe

35 40 45

Trp Gln Asn Leu Leu Asp Lys Arg Asp Ser Arg Ser Thr Leu Thr Asn

50 55 60

Glu Lys Leu Gly Ala Asn Ser Gln Asp Tyr Gln Gly Val Gln Gly Gln

65 70 75 80

Ser Asp Arg Phe Tyr Cys Asn Lys Gly Gly Tyr Ile Glu Asn Phe Ser

85 90 95

Phe Asn Ala Ala Gly Tyr Lys Leu Pro Glu Gln Ser Leu Asn Gly Leu

100 105 110

Asp Asp Ser Phe Leu Trp Ala Leu Asp Thr Ser Arg Asn Ala Leu Ile

115 120 125

Asp Ala Gly Ile Asp Ile Asn Gly Ala Asp Leu Ser Arg Ala Gly Val

130 135 140

Val Met Gly Ala Leu Ser Phe Pro Thr Thr Arg Ser Asn Asp Leu Phe

145 150 155 160

Leu Pro Ile Tyr His Ser Ala Val Glu Lys Ala Leu Gln Asp Lys Leu

165 170 175

Gly Val Lys Ala Phe Lys Leu Ser Pro Thr Asn Ala His Thr Ala Arg

180 185 190

Ala Ala Asn Glu Ser Ser Leu Asn Ala Ala Asn Gly Ala Ile Ala His

195 200 205

Asn Ser Ser Lys Val Val Ala Asp Ala Leu Gly Leu Gly Gly Ala Gln

210 215 220

Leu Ser Leu Asp Ala Ala Cys Ala Ser Ser Val Tyr Ser Leu Lys Leu

225 230 235 240

Ala Cys Asp Tyr Leu Ser Thr Gly Lys Ala Asp Ile Met Leu Ala Gly

245 250 255

Ala Val Ser Gly Ala Asp Pro Phe Phe Ile Asn Met Gly Phe Ser Ile

260 265 270

Phe His Ala Tyr Pro Asp His Gly Ile Ser Val Pro Phe Asp Ala Ser

275 280 285

Ser Lys Gly Leu Phe Ala Gly Glu Gly Ala Gly Val Leu Val Leu Lys

290 295 300

Arg Leu Glu Asp Ala Glu Arg Asp Asn Asp Lys Ile Tyr Ala Val Val

305 310 315 320

Ser Gly Val Gly Leu Ser Asn Asp Gly Lys Gly Gln Phe Val Leu Ser

325 330 335

Pro Asn Pro Lys Gly Gln Val Lys Ala Phe Glu Arg Ala Tyr Ala Ala

340 345 350

Ser Asp Ile Glu Pro Lys Asp Ile Glu Val Ile Glu Cys His Ala Thr

355 360 365

Gly Thr Pro Leu Gly Asp Lys Ile Glu Leu Thr Ser Met Glu Thr Phe

370 375 380

Phe Glu Asp Lys Leu Gln Gly Thr Asp Ala Pro Leu Ile Gly Ser Ala

385 390 395 400

Lys Ser Asn Leu Gly His Leu Leu Thr Ala Ala His Ala Gly Ile Met

405 410 415

Lys Met Ile Phe Ala Met Lys Glu Gly Tyr Leu Pro Pro Ser Ile Asn

420 425 430

Ile Ser Asp Ala Ile Ala Ser Pro Lys Lys Leu Phe Gly Lys Pro Thr

435 440 445

Leu Pro Ser Met Val Gln Gly Trp Pro Asp Lys Pro Ser Asn Asn His

450 455 460

Phe Gly Val Arg Thr Arg His Ala Gly Val Ser Val Phe Gly Phe Gly

465 470 475 480

Gly Cys Asn Ala His Leu Leu Leu Glu Ser Tyr Asn Gly Lys Gly Thr

485 490 495

Val Lys Ala Glu Ala Thr Gln Val Pro Arg Gln Ala Glu Pro Leu Lys

500 505 510

Val Val Gly Leu Ala Ser His Phe Gly Pro Leu Ser Ser Ile Asn Ala

515 520 525

Leu Asn Asn Ala Val Thr Gln Asp Gly Asn Gly Phe Ile Glu Leu Pro

530 535 540

Lys Lys Arg Trp Lys Gly Leu Glu Lys His Ser Glu Leu Leu Ala Glu

545 550 555 560

Phe Gly Leu Ala Ser Ala Pro Lys Gly Ala Tyr Val Asp Asn Phe Glu

565 570 575

Leu Asp Phe Leu Arg Phe Lys Leu Pro Pro Asn Glu Asp Asp Arg Leu

580 585 590

Ile Ser Gln Gln Leu Met Leu Met Arg Val Thr Asp Glu Ala Ile Arg

595 600 605

Asp Ala Lys Leu Glu Pro Gly Gln Lys Val Ala Val Leu Val Ala Met

610 615 620

Glu Thr Glu Leu Glu Leu His Gln Phe Arg Gly Arg Val Asn Leu His

625 630 635 640

Thr Gln Leu Ala Gln Ser Leu Ala Ala Met Gly Val Ser Leu Ser Thr

645 650 655

Asp Glu Tyr Gln Ala Leu Glu Ala Ile Ala Met Asp Ser Val Leu Asp

660 665 670

Ala Ala Lys Leu Asn Gln Tyr Thr Ser Phe Ile Gly Asn Ile Met Ala

675 680 685

Ser Arg Val Ala Ser Leu Trp Asp Phe Asn Gly Pro Ala Phe Thr Ile

690 695 700

Ser Ala Ala Glu Gln Ser Val Ser Arg Cys Ile Asp Val Ala Gln Asn

705 710 715 720

Leu Ile Met Glu Asp Asn Leu Asp Ala Val Val Ile Ala Ala Val Asp

725 730 735

Leu Ser Gly Ser Phe Glu Gln Val Ile Leu Lys Asn Ala Ile Ala Pro

740 745 750

Val Ala Ile Glu Pro Asn Leu Glu Ala Ser Leu Asn Pro Thr Ser Ala

755 760 765

Ser Trp Asn Val Gly Glu Gly Ala Gly Ala Val Val Leu Val Lys Asn

770 775 780

Glu Ala Thr Ser Gly Cys Ser Tyr Gly Gln Ile Asp Ala Leu Gly Phe

785 790 795 800

Ala Lys Thr Ala Glu Thr Ala Leu Ala Thr Asp Lys Leu Leu Ser Gln

805 810 815

Thr Ala Thr Asp Phe Asn Lys Val Lys Val Ile Glu Thr Met Ala Ala

820 825 830

Pro Ala Ser Gln Ile Gln Leu Ala Pro Ile Val Ser Ser Gln Val Thr

835 840 845

His Thr Ala Ala Glu Gln Arg Val Gly His Cys Phe Ala Ala Ala Gly

850 855 860

Met Ala Ser Leu Leu His Gly Leu Leu Asn Leu Asn Thr Val Ala Gln

865 870 875 880

Thr Asn Lys Ala Asn Cys Ala Leu Ile Asn Asn Ile Ser Glu Asn Gln

885 890 895

Leu Ser Gln Leu Leu Ile Ser Gln Thr Ala Ser Glu Gln Gln Ala Leu

900 905 910

Thr Ala Arg Leu Ser Asn Glu Leu Lys Ser Asp Ala Lys His Gln Leu

915 920 925

Val Lys Gln Val Thr Leu Gly Gly Arg Asp Ile Tyr Gln His Ile Val

930 935 940

Asp Thr Pro Leu Ala Ser Leu Glu Ser Ile Thr Gln Lys Leu Ala Gln

945 950 955 960

Ala Thr Ala Ser Thr Val Val Asn Gln Val Lys Pro Ile Lys Ala Ala

965 970 975

Gly Ser Val Glu Met Ala Asn Ser Phe Glu Thr Glu Ser Ser Ala Glu

980 985 990

Pro Gln Ile Thr Ile Ala Ala Gln Gln Thr Ala Asn Ile Gly Val Thr

995 1000 1005

Ala Gln Ala Thr Lys Arg Glu Leu Gly Thr Pro Pro Met Thr Thr Asn

1010 1015 1020

Thr Ile Ala Asn Thr Ala Asn Asn Leu Asp Lys Thr Leu Glu Thr Val

1025 1030 1035 1040

Ala Gly Asn Thr Val Ala Ser Lys Val Gly Ser Gly Asp Ile Val Asn

1045 1050 1055

Phe Gln Gln Asn Gln Gln Leu Ala Gln Gln Ala His Leu Ala Phe Leu

1060 1065 1070

Glu Ser Arg Ser Ala Gly Met Lys Val Ala Asp Ala Leu Leu Lys Gln

1075 1080 1085

Gln Leu Ala Gln Val Thr Gly Gln Thr Ile Asp Asn Gln Ala Leu Asp

1090 1095 1100

Thr Gln Ala Val Asp Thr Gln Thr Ser Glu Asn Val Ala Ile Ala Ala

1105 1110 1115 1120

Glu Ser Pro Val Gln Val Thr Thr Pro Val Gln Val Thr Thr Pro Val

1125 1130 1135

Gln Ile Ser Val Val Glu Leu Lys Pro Asp His Ala Asn Val Pro Pro

1140 1145 1150

Tyr Thr Pro Pro Val Pro Ala Leu Lys Pro Cys Ile Trp Asn Tyr Ala

1155 1160 1165

Asp Leu Val Glu Tyr Ala Glu Gly Asp Ile Ala Lys Val Phe Gly Ser

1170 1175 1180

Asp Tyr Ala Ile Ile Asp Ser Tyr Ser Arg Arg Val Arg Leu Pro Thr

1185 1190 1195 1200

Thr Asp Tyr Leu Leu Val Ser Arg Val Thr Lys Leu Asp Ala Thr Ile

1205 1210 1215

Asn Gln Phe Lys Pro Cys Ser Met Thr Thr Glu Tyr Asp Ile Pro Val

1220 1225 1230

Asp Ala Pro Tyr Leu Val Asp Gly Gln Ile Pro Trp Ala Val Ala Val

1235 1240 1245

Glu Ser Gly Gln Cys Asp Leu Met Leu Ile Ser Tyr Leu Gly Ile Asp

1250 1255 1260

Phe Glu Asn Lys Gly Glu Arg Val Tyr Arg Leu Leu Asp Cys Thr Leu

1265 1270 1275 1280

Thr Phe Leu Gly Asp Leu Pro Arg Gly Gly Asp Thr Leu Arg Tyr Asp

1285 1290 1295

Ile Lys Ile Asn Asn Tyr Ala Arg Asn Gly Asp Thr Leu Leu Phe Phe

1300 1305 1310

Phe Ser Tyr Glu Cys Phe Val Gly Asp Lys Met Ile Leu Lys Met Asp

1315 1320 1325

Gly Gly Cys Ala Gly Phe Phe Thr Asp Glu Glu Leu Ala Asp Gly Lys

1330 1335 1340

Gly Val Ile Arg Thr Glu Glu Glu Ile Lys Ala Arg Ser Leu Val Gln

1345 1350 1355 1360

Lys Gln Arg Phe Asn Pro Leu Leu Asp Cys Pro Lys Thr Gln Phe Ser

1365 1370 1375

Tyr Gly Asp Ile His Lys Leu Leu Thr Ala Asp Ile Glu Gly Cys Phe

1380 1385 1390

Gly Pro Ser His Ser Gly Val His Gln Pro Ser Leu Cys Phe Ala Ser

1395 1400 1405

Glu Lys Phe Leu Met Ile Glu Gln Val Ser Lys Val Asp Arg Thr Gly

1410 1415 1420

Gly Thr Trp Gly Leu Gly Leu Ile Glu Gly His Lys Gln Leu Glu Ala

1425 1430 1435 1440

Asp His Trp Tyr Phe Pro Cys His Phe Lys Gly Asp Gln Val Met Ala

1445 1450 1455

Gly Ser Leu Met Ala Glu Gly Cys Gly Gln Leu Leu Gln Phe Tyr Met

1460 1465 1470

Leu His Leu Gly Met His Thr Gln Thr Lys Asn Gly Arg Phe Gln Pro

1475 1480 1485

Leu Glu Asn Ala Ser Gln Gln Val Arg Cys Arg Gly Gln Val Leu Pro

1490 1495 1500

Gln Ser Gly Val Leu Thr Tyr Arg Met Glu Val Thr Glu Ile Gly Phe

1505 1510 1515 1520

Ser Pro Arg Pro Tyr Ala Lys Ala Asn Ile Asp Ile Leu Leu Asn Gly

1525 1530 1535

Lys Ala Val Val Asp Phe Gln Asn Leu Gly Val Met Ile Lys Glu Glu

1540 1545 1550

Asp Glu Cys Thr Arg Tyr Pro Leu Leu Thr Glu Ser Thr Thr Ala Ser

1555 1560 1565

Thr Ala Gln Val Asn Ala Gln Thr Ser Ala Lys Lys Val Tyr Lys Pro

1570 1575 1580

Ala Ser Val Asn Ala Pro Leu Met Ala Gln Ile Pro Asp Leu Thr Lys

1585 1590 1595 1600

Glu Pro Asn Lys Gly Val Ile Pro Ile Ser His Val Glu Ala Pro Ile

1605 1610 1615

Thr Pro Asp Tyr Pro Asn Arg Val Pro Asp Thr Val Pro Phe Thr Pro

1620 1625 1630

Tyr His Met Phe Glu Phe Ala Thr Gly Asn Ile Glu Asn Cys Phe Gly

1635 1640 1645

Pro Glu Phe Ser Ile Tyr Arg Gly Met Ile Pro Pro Arg Thr Pro Cys

1650 1655 1660

Gly Asp Leu Gln Val Thr Thr Arg Val Ile Glu Val Asn Gly Lys Arg

1665 1670 1675 1680

Gly Asp Phe Lys Lys Pro Ser Ser Cys Ile Ala Glu Tyr Glu Val Pro

1685 1690 1695

Ala Asp Ala Trp Tyr Phe Asp Lys Asn Ser His Gly Ala Val Met Pro

1700 1705 1710

Tyr Ser Ile Leu Met Glu Ile Ser Leu Gln Pro Asn Gly Phe Ile Ser

1715 1720 1725

Gly Tyr Met Gly Thr Thr Leu Gly Phe Pro Gly Leu Glu Leu Phe Phe

1730 1735 1740

Arg Asn Leu Asp Gly Ser Gly Glu Leu Leu Arg Glu Val Asp Leu Arg

1745 1750 1755 1760

Gly Lys Thr Ile Arg Asn Asp Ser Arg Leu Leu Ser Thr Val Met Ala

1765 1770 1775

Gly Thr Asn Ile Ile Gln Ser Phe Ser Phe Glu Leu Ser Thr Asp Gly

1780 1785 1790

Glu Pro Phe Tyr Arg Gly Thr Ala Val Phe Gly Tyr Phe Lys Gly Asp

1795 1800 1805

Ala Leu Lys Asp Gln Leu Gly Leu Asp Asn Gly Lys Val Thr Gln Pro

1810 1815 1820

Trp His Val Ala Asn Gly Val Ala Ala Ser Thr Lys Val Asn Leu Leu

1825 1830 1835 1840

Asp Lys Ser Cys Arg His Phe Asn Ala Pro Ala Asn Gln Pro His Tyr

1845 1850 1855

Arg Leu Ala Gly Gly Gln Leu Asn Phe Ile Asp Ser Val Glu Ile Val

1860 1865 1870

Asp Asn Gly Gly Thr Glu Gly Leu Gly Tyr Leu Tyr Ala Glu Arg Thr

1875 1880 1885

Ile Asp Pro Ser Asp Trp Phe Phe Gln Phe His Phe His Gln Asp Pro

1890 1895 1900

Val Met Pro Gly Ser Leu Gly Val Glu Ala Ile Ile Glu Thr Met Gln

1905 1910 1915 1920

Ala Tyr Ala Ile Ser Lys Asp Leu Gly Ala Asp Phe Lys Asn Pro Lys

1925 1930 1935

Phe Gly Gln Ile Leu Ser Asn Ile Lys Trp Lys Tyr Arg Gly Gln Ile

1940 1945 1950

Asn Pro Leu Asn Lys Gln Met Ser Met Asp Val Ser Ile Thr Ser Ile

1955 1960 1965

Lys Asp Glu Asp Gly Lys Lys Val Ile Thr Gly Asn Ala Ser Leu Ser

1970 1975 1980

Lys Asp Gly Leu Arg Ile Tyr Glu Val Phe Asp Ile Ala Ile Ser Ile

1985 1990 1995 2000

Glu Glu Ser Val

10

543

Shewanella putrefaciens

10

Met Asn Pro Thr Ala Thr Asn Glu Met Leu Ser Pro Trp Pro Trp Ala

1 5 10 15

Val Thr Glu Ser Asn Ile Ser Phe Asp Val Gln Val Met Glu Gln Gln

20 25 30

Leu Lys Asp Phe Ser Arg Ala Cys Tyr Val Val Asn His Ala Asp His

35 40 45

Gly Phe Gly Ile Ala Gln Thr Ala Asp Ile Val Thr Glu Gln Ala Ala

50 55 60

Asn Ser Thr Asp Leu Pro Val Ser Ala Phe Thr Pro Ala Leu Gly Thr

65 70 75 80

Glu Ser Leu Gly Asp Asn Asn Phe Arg Arg Val His Gly Val Lys Tyr

85 90 95

Ala Tyr Tyr Ala Gly Ala Met Ala Asn Gly Ile Ser Ser Glu Glu Leu

100 105 110

Val Ile Ala Leu Gly Gln Ala Gly Ile Leu Cys Gly Ser Phe Gly Ala

115 120 125

Ala Gly Leu Ile Pro Ser Arg Val Glu Ala Ala Ile Asn Arg Ile Gln

130 135 140

Ala Ala Leu Pro Asn Gly Pro Tyr Met Phe Asn Leu Ile His Ser Pro

145 150 155 160

Ser Glu Pro Ala Leu Glu Arg Gly Ser Val Glu Leu Phe Leu Lys His

165 170 175

Lys Val Arg Thr Val Glu Ala Ser Ala Phe Leu Gly Leu Thr Pro Gln

180 185 190

Ile Val Tyr Tyr Arg Ala Ala Gly Leu Ser Arg Asp Ala Gln Gly Lys

195 200 205

Val Val Val Gly Asn Lys Val Ile Ala Lys Val Ser Arg Thr Glu Val

210 215 220

Ala Glu Lys Phe Met Met Pro Ala Pro Ala Lys Met Leu Gln Lys Leu

225 230 235 240

Val Asp Asp Gly Ser Ile Thr Ala Glu Gln Met Glu Leu Ala Gln Leu

245 250 255

Val Pro Met Ala Asp Asp Ile Thr Ala Glu Ala Asp Ser Gly Gly His

260 265 270

Thr Asp Asn Arg Pro Leu Val Thr Leu Leu Pro Thr Ile Leu Ala Leu

275 280 285

Lys Glu Glu Ile Gln Ala Lys Tyr Gln Tyr Asp Thr Pro Ile Arg Val

290 295 300

Gly Cys Gly Gly Gly Val Gly Thr Pro Asp Ala Ala Leu Ala Thr Phe

305 310 315 320

Asn Met Gly Ala Ala Tyr Ile Val Thr Gly Ser Ile Asn Gln Ala Cys

325 330 335

Val Glu Ala Gly Ala Ser Asp His Thr Arg Lys Leu Leu Ala Thr Thr

340 345 350

Glu Met Ala Asp Val Thr Met Ala Pro Ala Ala Asp Met Phe Glu Met

355 360 365

Gly Val Lys Leu Gln Val Val Lys Arg Gly Thr Leu Phe Pro Met Arg

370 375 380

Ala Asn Lys Leu Tyr Glu Ile Tyr Thr Arg Tyr Asp Ser Ile Glu Ala

385 390 395 400

Ile Pro Leu Asp Glu Arg Glu Lys Leu Glu Lys Gln Val Phe Arg Ser

405 410 415

Ser Leu Asp Glu Ile Trp Ala Gly Thr Val Ala His Phe Asn Glu Arg

420 425 430

Asp Pro Lys Gln Ile Glu Arg Ala Glu Gly Asn Pro Lys Arg Lys Met

435 440 445

Ala Leu Ile Phe Arg Trp Tyr Leu Gly Leu Ser Ser Arg Trp Ser Asn

450 455 460

Ser Gly Glu Val Gly Arg Glu Met Asp Tyr Gln Ile Trp Ala Gly Pro

465 470 475 480

Ala Leu Gly Ala Phe Asn Gln Trp Ala Lys Gly Ser Tyr Leu Asp Asn

485 490 495

Tyr Gln Asp Arg Asn Ala Val Asp Leu Ala Lys His Leu Met Tyr Gly

500 505 510

Ala Ala Tyr Leu Asn Arg Ile Asn Ser Leu Thr Ala Gln Gly Val Lys

515 520 525

Val Pro Ala Gln Leu Leu Arg Trp Lys Pro Asn Gln Arg Met Ala

530 535 540

11

499

Shewanella putrefaciens

11

Met Arg Lys Pro Leu Gln Thr Ile Asn Tyr Asp Tyr Ala Val Trp Asp

1 5 10 15

Arg Thr Tyr Ser Tyr Met Lys Ser Asn Ser Ala Ser Ala Lys Arg Tyr

20 25 30

Tyr Glu Lys His Glu Tyr Pro Asp Asp Thr Phe Lys Ser Leu Lys Val

35 40 45

Asp Gly Val Phe Ile Phe Asn Arg Thr Asn Gln Pro Val Phe Ser Lys

50 55 60

Gly Phe Asn His Arg Asn Asp Ile Pro Leu Val Phe Glu Leu Thr Asp

65 70 75 80

Phe Lys Gln His Pro Gln Asn Ile Ala Leu Ser Pro Gln Thr Lys Gln

85 90 95

Ala His Pro Pro Ala Ser Lys Pro Leu Asp Ser Pro Asp Asp Val Pro

100 105 110

Ser Thr His Gly Val Ile Ala Thr Arg Tyr Gly Pro Ala Ile Tyr Tyr

115 120 125

Ser Ser Thr Ser Ile Leu Lys Ser Asp Arg Ser Gly Ser Gln Leu Gly

130 135 140

Tyr Leu Val Phe Ile Arg Leu Ile Asp Glu Trp Phe Ile Ala Glu Leu

145 150 155 160

Ser Gln Tyr Thr Ala Ala Gly Val Glu Ile Ala Met Ala Asp Ala Ala

165 170 175

Asp Ala Gln Leu Ala Arg Leu Gly Ala Asn Thr Lys Leu Asn Lys Val

180 185 190

Thr Ala Thr Ser Glu Arg Leu Ile Thr Asn Val Asp Gly Lys Pro Leu

195 200 205

Leu Lys Leu Val Leu Tyr His Thr Asn Asn Gln Pro Pro Pro Met Leu

210 215 220

Asp Tyr Ser Ile Ile Ile Leu Leu Val Glu Met Ser Phe Leu Leu Ile

225 230 235 240

Leu Ala Tyr Phe Leu Tyr Ser Tyr Phe Leu Val Arg Pro Val Arg Lys

245 250 255

Leu Ala Ser Asp Ile Lys Lys Met Asp Lys Ser Arg Glu Ile Lys Lys

260 265 270

Leu Arg Tyr His Tyr Pro Ile Thr Glu Leu Val Lys Val Ala Thr His

275 280 285

Phe Asn Ala Leu Met Gly Thr Ile Gln Glu Gln Thr Lys Gln Leu Asn

290 295 300

Glu Gln Val Phe Ile Asp Lys Leu Thr Asn Ile Pro Asn Arg Arg Ala

305 310 315 320

Phe Glu Gln Arg Leu Glu Thr Tyr Cys Gln Leu Leu Ala Arg Gln Gln

325 330 335

Ile Gly Phe Thr Leu Ile Ile Ala Asp Val Asp His Phe Lys Glu Tyr

340 345 350

Asn Asp Thr Leu Gly His Leu Ala Gly Asp Glu Ala Leu Ile Lys Val

355 360 365

Ala Gln Thr Leu Ser Gln Gln Phe Tyr Arg Ala Glu Asp Ile Cys Ala

370 375 380

Arg Phe Gly Gly Glu Glu Phe Ile Met Leu Phe Arg Asp Ile Pro Asp

385 390 395 400

Glu Pro Leu Gln Arg Lys Leu Asp Ala Met Leu His Ser Phe Ala Glu

405 410 415

Leu Asn Leu Pro His Pro Asn Ser Ser Thr Ala Asn Tyr Val Thr Val

420 425 430

Ser Leu Gly Val Cys Thr Val Val Ala Val Asp Asp Phe Glu Phe Lys

435 440 445

Ser Glu Ser His Ile Ile Gly Ser Gln Ala Ala Leu Ile Ala Asp Lys

450 455 460

Ala Leu Tyr His Ala Lys Ala Cys Gly Arg Asn Gln Ala Leu Ser Lys

465 470 475 480

Thr Thr Ile Thr Val Asp Glu Ile Glu Gln Leu Glu Ala Asn Lys Ile

485 490 495

Gly His Gln

12

40138

Vibrio marinus

12

aatagatcga ctcgcaaaag ttgcttaaga tagtgtcaat atagcttctt atttgtaaat 60

attgtttttt atgtgtaaac atgtttagtg tgtgtaaatg ctgttaatta tccttttggg 120

attgtaatag ctgatgttgc tggctaatga gtacttttag ttcggcaata tcttgcttta 180

aatcgctaac ttcagttttt aattcaccca cacttgttgt atttttaagg ctctcttccc 240

caccatcgac aaaccaggat gatatgaaac cggtaaacgt accaaagaga ccgacacctg 300

cagtcatgag taatgccgca atgatacgtc cgccagtggt gacggggtag tagtcaccgt 360

aaccaacagt cgttattgtc acaaatgacc accaaagtgc gtcgatgccg ttattgatgt 420

tactgcctac ttgatcctgt tctaacaata aaataccgat agcaccaaag gtgacaagga 480

tgaaggatat cgcagatacc agcgaaaagg tggctttaaa ccgatgttca aaaatcattt 540

ttaagataat ttttgatgag cgtatattct gaatagatct taatactcta gcgatacgaa 600

ttatgcgaat aaactgcagt tgctcgacca tcggaatact cgacagtagg tcaatccaac 660

cccatttcat aaactgaaat ttattctcag cttggtgaaa gcgaattaca aagtcagtga 720

aaaagaataa gcaaatcgta ttatctacgc tcgttaatat ttcagtgacg ttacttgaaa 780

aggtaaaaat aagttgcagt agtgatgata cgaccacatg aagtgataaa ataagcatga 840

aaatctgaaa tggatttaca tcactgttgt ttttggtgcc acttttaagg ttcgttttca 900

caatctgctg cctcggttca ttgattttgt taatataaac cttagtcagt agcaagacaa 960

aatatattta catcaatgtc atcgtattat tcaaccgcgc gtcgtgtatt cagaccaaga 1020

tcgttgtata tgttagtcat gtagcgatga gattatcatg cgacaggaga gaattatgtt 1080

tgttattatt ttttacgtac ctaaagttaa tgttgaagaa gtaaaacagg cgttatttaa 1140

cgtcggagct ggcaccatcg gtgattatga tagttgtgct tggcaatgtt tggggactgg 1200

gcagttccaa cctttacttg gtagccagcc acatattggt aagctaaatg aggttgaatt 1260

cgttgatgag tttagagtag aaatggtttg tcgagcagaa aatgtaaggg cagcaataaa 1320

tgcacttatt gctgcgcacc cttatgaaga acctgcttat catattctgc aaacattgaa 1380

tcttgatgag ttaccttaag ttagatgcac tgcacttaat tggttcgctg tgctaggtta 1440

gcaattagca attttgacca tgttagcgat agttttggca caagtgatcg atattaaact 1500

atccgattca gatcccattt ttactgctga attaggtttc attacacttg ttctagtggt 1560

ttttcccgac aggtgtaact ctgttacttg cgtaaggttg ataatctcta ccgcattggc 1620

aggagttaca cctgcaccag gcataatact aattctacca tctgcttggt taactaacgt 1680

ttggattaag gcgcagcctt ctagcgcttg agcttgttga ccagaggtta aaatacgctc 1740

acaaccagca gtgatcaagg tctccaaggc ttgttgtgga tcattacaca agtcgaaagc 1800

gcggtggaag gttacgccga gatcacgtga tgccaccatt aagcgtttta aagctggctc 1860

gtcaatatta ccatctgctg ttaacgcgcc aataacgacc ccttggacac cgagtaactt 1920

catgaatttg atgtcggaaa ccataatatc aacttcttgt tcgctatata caaaatcacc 1980

ggcgcgaggg cgaataatgg cataaatggg gatcgttgct agatcaatag acttttgtac 2040

aaaacctgcg ttggcggtca agccacctaa tgctaatgcc gagcacaact caatacgatc 2100

ggcgccagat gcttgagccg tcagcagtga ttctatatta tcgacacata cttctattgt 2160

cattgtcata tacttctctt taaaaagttt attaaaaata ataaagccag cataagtcgt 2220

tttatacaat atgaaagggg aaaaggcgac ttagctcgcc tagatcaatt attatggcag 2280

aatactgccg tattgtgatt agaaagacag ttttttaagc tcaatagccg ttatcgcgtt 2340

gttatctacc atcgtgtaac ttttctggcc tgggtgcttt attaacactg tttcagtggc 2400

tggattaggg tgaaatgatt cttttttcaa atctgttttt ttgtatttga acgtacctgt 2460

aatgtcttgc tgctcacgaa gacgtacaaa tattggttgc gcatagcttg gtagtgccgc 2520

attgacatgt tgatagaatt cagacgctga aaattcatga atagggcaat tcaaagtcag 2580

cgcgaccatg cctgctcggc catcgtgatg tgggagcttg acaccataag ccacactttg 2640

ctcaatttgc acaaaatcgt taacttgagc ttctacttgc gtcgtggcga cattttcacc 2700

tttccagcgg aatgtatcac ctaatctatc cacaaaggaa atatggcgat aaccttggta 2760

atgaacgaga tcgccggtat taaaataaca gtcaccgtct tttaatactg acttaaatag 2820

ctttttatta ctttcgttgt catcggtata accatcaaat ggtgaacgtt tagttatctt 2880

tgttagcagt agccctgttt ctcccgtttt tactttggtc attttccctt tcgcattata 2940

cacaggtttg tcattgtcaa tatcatattg tatgacggta aaagcaagtg gagtaacccc 3000

cgctgtatgc ggtaagttca gcgcattgga gaacacaaga ttacactcac tggcgccata 3060

gaattcatta atatgctcga tcccaaaacg ttgttggaaa tgatcccaaa tttcggggcg 3120

taatccatta cctatgattt tctttatatt atgctgtttg tctttattgc taggcggtac 3180

atttaataaa taacggcaga gctcgccgat gtaagtaaac gcagtggcat tatgagcacg 3240

aacttcatcc caaaagcgac ttgaactgaa tttttcagaa agtgcgaggg ttgctgcgct 3300

accaaacacg gcgcttaatg acactgtcag tgcattgtta tggtataggg ggagtgataa 3360

atacaataca tcatcagctg ttaagcgtaa tgatgccatc cccatgcctg ccatggattt 3420

aaaccaacgg tgatggctca ttcttgctgc ttttggcagt ccagtttttc ccgaggtaaa 3480

gatataaaac gcgcaatgct taagctgtat ttgtgctgtt gattcagggt tcaatactga 3540

atatcctgcg actagtgtag atatgttttt ataaccatca ctcatgtctg gcgtttctaa 3600

agcgggtacg taaaagacat tctgttgtaa tgtcgatgac aaattggttt caatattatt 3660

aatggcggat gtgtatagtt catctgcgat gagtaatttg gtatcgacca cgctaagact 3720

atgttcgagg attgaatccc gttgtgtcgt atttatcata caagcaatcg cgccaagctt 3780

gacaactgcg agggcaataa tgatggtttc aggcctgtta tcgagcatga tggcgacttt 3840

atcattttta ccaatgccgt attcatgaag gaaatgggca tattgatttg cttgcttatt 3900

caatgaatcg taactataac gctggtcttt aaattgtatt gcgatcaagt cagagttatt 3960

gacagcttgc tgctctagta ataaaccaat agacataaaa cgttcgggct ttgcttgttg 4020

taagtgccat aagcctttga tgattggctt tggggttttt aatagattga tggtactttt 4080

caggaattgt ttgccggtta taacagtcat aagctaattc tttttatcaa gaagaggggt 4140

tatgacacca aataaatggg tcacgcgttg gtttaatttg gttagactaa atgtgttgtt 4200

ttgctgtgat aatgcgacgt tcaaacaaac ttgagaaggt aaaaaaatag catttttaaa 4260

ttgaacatca atactaatgt gttgaatatc aatcaagttt tctaactgtg cgagcacgcg 4320

tgctttagca aacatgccat gtgctattgc tgttttaaac cccattagtt tcgctgggat 4380

aaaatgtaaa tggattggat ttgtgtcttt ggagatataa gcatatttat atacgtcaaa 4440

aggactaaat ttaaacaatg aaatcggctc gtaagcataa ttcgctggcg tatttactat 4500

tttctcaccg ctggaacgtt gagatcgttg gcacgttttt cgctgtttcg ttttctgtaa 4560

gaatgtcgat gtacactccc acgcaaattg tccatctaca aacacatcaa tatgagtatc 4620

aatgaaacgt cctgtatccg ttatgtactc cttaattaca cgacatgtgc tcgtcaatat 4680

cgcgtttaat gctatcggtt gatgttgtgt tatgcgattt cgataatgga ctagtcctaa 4740

tatagatatc ggaaattgtg ttgatgtcat gagtttcatc aataatggaa agatcatcac 4800

aaatggataa gtaaccggta catagtttgt gttattaaac ccacagcatt taatatattg 4860

ctttaaattt cgctgatcta ttttttgtcc actgatacta aattgctcag tacacacttg 4920

tgtcgaccaa gtgttcatca gtgttttaac aattgtattg accactgctt tcacatataa 4980

aagcgagata atcggttgct ttgttaacag tgtgatctgg ttagcgtgca ttgaaataat 5040

tcatataaga gtatgtagca tttatgttaa tattttgttt tggaagttga attggcgaat 5100

ccgtaatcgg tttatggcag ttcggtcaaa tacttcaggt aaactcgtta ctcataccat 5160

tgatagtgtt aaagtgattg actgaataaa gaatagagct aaaagtggaa aaattatgca 5220

agatgcgggt atgttattac gcattgctta tgaggcaatg aaagagttag aggttgatgt 5280

cattgaagta ctttctcgtt gtaacataag tgaagaagta ctgaatgata aggatcttcg 5340

cacacctaat catgcacaaa cacatttttg gcaagtatta gaagacatat cacaagatcc 5400

taacatcggc atttcacttg gtgagagaat gccagtgttc acggggcagg tattacagta 5460

tctttttctc agtagtccta catttggtac tggctgggaa cgcgcaacaa aatactttcg 5520

attaatcagt gatgcggcga gtgtttctat caagatggaa ggctgtgaag cgcgattatc 5580

tgtgaactta gatggtttag cggaagatgc gaatcgtcat ttgaatgatt gcctagtgat 5640

cggtgcattt aaattttgtt tatatgtgac agaaggcgaa tttaaagtaa gcaaaatagc 5700

ctttgctcat gctcgcccga aagatattac tgcctatacc aatgtattta catgtccgat 5760

tgagtttgct gccgaagata attatattta tttcgatgct gatttactcg aacgtccttc 5820

ttcgcatgcg gagcctgagc tattcgcctt acacgatcag cttgcaagcc gtaaaatagc 5880

caagttagaa ctgcaagatt tagtggataa agtacgtaag gttattgcac aacaacttga 5940

gtctggtgtg gtgactttag aaagtatcgc cactgaactt gacatgaaac cacgtatgct 6000

aagagcgaag ttagctgaca ttgattataa ctttaatcaa atactcgctg attttcgttg 6060

cgagttatca aaaaaactgt tggcgaatac ggacgagtct attgatcaga ttgtctatct 6120

cactggtttt tctgaaccaa gtacttttta tcgtgccttt aagcgctggg ttaaaatgac 6180

gccaattgaa tatcgccgta gcaaactcgc ggttaggcat gctaatcaac acgagtccta 6240

aaaattcgct gcttagtgca tagtgcatag tgcatagtgc tagtaagcca agtacaaagc 6300

gttaaagtta agtacttgag cgaaccatca gacaccactt actagattaa gcacctatta 6360

atgattgacc acaaattctg atcgtattgc ctgtgatccc tgcagcttga ggttgcgcaa 6420

aaaaagctat cgcttcagca acatcaactg gcttaccacc ttgttttaat gaattcatac 6480

gacgaccagc ttcacgaact gtaaatggaa tcgctgctgt catttttgtt tcaataaagc 6540

ctggtgcaac agcattaatg gtgatgtatt tgtctgcaag cggagtttgc attgcatcaa 6600

cataaccaat gactgcggcc ttagacgttg cataattagt ctgaccaaag ttacccgcaa 6660

tcccactcat cgaagacaca caaacaatgc ggccatagtc gttgagcaga tcatcattta 6720

gcagtcgctc attgattctt tccattgccg acaagttaat atccatcagt acatcccaat 6780

ggttatccgg catacgtgct agcgttttgt cttttgttac cccggcatta tggacgatga 6840

tatcaagcga ctgttctcgc acaaagtcag caatgatatt tggggcgtca gcagcggtaa 6900

tatcagcaac aatgctgcta cctttcaagc aatgagctac tttttcaagg tcctgtttta 6960

atgccggaat gtctaagcaa ataacatgtg cgccatcacg ggcgagtgtt tcagcaatag 7020

cagccccgat gccacgtgat gcaccagtga caagtgctgt ctttccttgt aatggttttg 7080

ccgtgttact tgtttcgtta ataacttcgt taataacttc gttaataact tcgttaatag 7140

ccccattaat cgaaccgggt tttacgttaa taacctgtgc tgagatatag gctgattttg 7200

ctgaggttaa gaaacgtagc ggggcctcta ataattgctc actaccaggt tgtacataga 7260

taagttgaca ggtactacca ttcttgccta tttctttggc gacactgcga caaaaccctt 7320

ctaaagatct ttgtacagtc gcgtagctta catcgtcaag atgttcactc ggatgaccta 7380

acacgatcac tctgctgcat ggcgagagct gcttaattac aggttgaaaa aaacgatgta 7440

atgcacttaa ttgcttgctg ttcttaatgc ctgaggcgtc gaagataata ccgttgaagc 7500

gatctgtttt agcgatagca ttaaggctaa taggtgtcgc gactaaagac gtttgattaa 7560

attcaatatt aagatcggct aacgctgacg tgttattagg ataagaaatc gtgacttcag 7620

catctttaaa tgtgttaaga atgggtttaa ttaatttgct gttgctggct gcgccgatga 7680

gtaagttgcc agagatgaga tcggttccct gatcgtagcg tgttaacgta accggtcgtg 7740

gcagattaag cgctttaaat aaacctgatg tccacttgcc attagcgagt tttgcgtatg 7800

tatccgtcat tttctaatcc ttgttatagt gaacagtttg aatctcgaag atgtacatgt 7860

gttaaaaatt atctgatagc tatgacttat ctgccactac gtaataataa atagaccagt 7920

tcattacatc gttaatcgat atagtataac taaatactaa gtaaattata atgataagac 7980

tgttatcgta ctcggatcaa actctgatca gcaaataatc aaattagagt ttttatttta 8040

aacttgtatc aacaatgtta cattaatgta tcttacgtct aatgtgctac gggcatattt 8100

aagtcactaa attaaaggaa taaaccatga caggtcaaac aataagaaga gtagcaatta 8160

tcggcggtaa ccgtatcccg tttgcacgtt caaatacagc gtattcaaaa ctaagtaacc 8220

aagatatgct gacggaaact atccgtggct tggtggttaa atataaccta cgtggtgaac 8280

aactggggga agttgttgct ggtgcggtaa ttaagcattc tcgtgatttt aacttaacac 8340

gtgaagccgt gctaagtgca ggtcttgcac ctgaaacgcc ttgttatgac attcaacaag 8400

cttgtggtac tggtctagct gcagctatcc aagtagcaaa caaaattgcg cttggtcaaa 8460

tagaagcggg tattgctggt ggttctgata cgacatcaga tgcaccgatt gcagtcagtg 8520

aaggcatgcg tagtgtatta cttgagctta atcgagctaa aacgggtaag caacgtttga 8580

aagcactatc tcgtctacgt ctaaaacact ttgcgccact aacgcctgca aataaagagc 8640

cgcgtaccaa aatggcgatg ggcgatcatt gtcaagtaac agcgaaagag tggaatatct 8700

cacgtgaagc acaagatgca ttggcctgcg caagtcatca aaaattagct gcagcatatg 8760

aagaaggttt ctttgatacg ttagtttcac ctatggccgg cttaacgaaa gataacgtat 8820

tacgcgcaga tacaacagtt gagaaactgg ctaaattgaa accttgtttt gataaagtaa 8880

acggcactat gacggcgggt aacagtacta accttaccga tggagcatca gctgtattac 8940

ttgcaagtga agaatgggca gcggcacata acttaccagt acaagcttat ctaacatttg 9000

gtgaaacggc cgctatcgac ttcgttgata agaaagaagg tctgttaatg gcgcctgcat 9060

acgcagtgcc aaaaatgttg aagcgtgctg gccttacatt acaagacttc gattactatg 9120

aaatacatga agcatttgct gcgcagttat tagcaacgct agcagcttgg gaagacgaaa 9180

aattctgtaa agaaaaactg ggtctagatg ctgcgcttgg ttcaattgat atgaccaagt 9240

taaacgtgaa agggagtagc ttagccacgg gtcacccatt tgccgcaact ggtggtcgtg 9300

ttgtcgctac gctagcgcaa ttacttgatc agaaaggttc aggtcgtggt ttgatctcga 9360

tttgtgctgc tggtggtcaa ggtatcacgg caattttaga gaaataaacg cactgtttat 9420

tatctattga ttaagctgtc ctgagatact ggatattttt aaataaaacg ccaatactgc 9480

agagtattgg cgtttttttg taataccaat tcctatataa cggtgcattt taaacactta 9540

atttccggca ttggtatcat aaaaaagcag caccgaagtg ctgcttgatt gtagattaac 9600

ctattaaaat agagaggcta gaattagtct tcgtatgctt cattatgtac gccagctgca 9660

cgacccgatg gatcagcatt gttttggaaa ctttcatccc aagctaatgc ttctacagtt 9720

gaacaagcaa cggatttacc aaacggtacg catttcgctg ctgaatcacc tgggaagtga 9780

tcttcaaaga tggcacgata gtagtaacct tctttcgtat ctggtgtgtt aattgggaac 9840

ttaaatgctg cacttgctaa catttgatca gttaccgctt cttcaacgtg tactttaagt 9900

tggtcaatcc aagaataacc aacaccatca gagaattgtt ctttttgacg ccatacaatt 9960

tcttcaggta gtaaatcttc aaatgcttct cgaatgatgt ttttctcaat gcggtcgccc 10020

gtgatcattt ttagttcagg gtttagacgc attgacgcat caacaaattc tttatctaag 10080

aaaggaacac gtgcttcgat gccccaagct gccatagatt tgtttgcacg taagcaatca 10140

aacatatgta atttatttac tttacgtacc gtctcttcat ggaattcttt cgcatttggc 10200

gctttgtgga agtacaagta accaccgaac agttcatcag caccttcacc agaaagcacc 10260

atcttaatcc ccatggcttt aattttacgt gccattaggt acataggggt tgatgcacga 10320

attgttgtta catcgtaggt ttcaatgtgg taaatcacgt cgcgtaaagc gtcgatacct 10380

tcttgcacag taaattcaat tgaatgatgg atagtaccta agtgatctgc cactttttgt 10440

gcagcggcta aatctggaga accatttagg cctacagaga aagagtgtag ttgtggccac 10500

catgcttcgg ttttaccacc gtcttcaata cgacgttttg catactgttg ggtgattgct 10560

gaaataacag atgaatctaa cccgcctgat aataatacgc cgtaaggtac atcacacatt 10620

aattgacgtt taactgcatc ttccaaacct tgcttaacaa cgcttttatc accaccattt 10680

tgtgcaacgt tatcaaaatc tttccaatca cgttgataat aaggcgtgac tacaccatcc 10740

ttactccaca ggtaatgacc tgctgggaat tcttcaattt gagtacaaat tggcactagt 10800

gctttcattt cagaggcaac ataaaagtta ccgtgttcat catagcccgt ataaagaggg 10860

atgataccga tatggtcacg gccaatcagg taagcgtcct ctgtttcgtc atataaagcg 10920

aaagcaaaaa taccatttag atcatctaaa aattgtgtgc ctttttcttt atatagcgca 10980

agtatcactt cgcaatctga ttctgtttgg aattcaaagt ctacgttcag cgttttcttt 11040

aaatctttgt ggttataaat ttcaccatta acagcaagta cgtgtgtctt ttcttcatta 11100

tatagcggct gtgcaccatt atttacatcg acaatagcaa gacgttcatg aactaaaata 11160

gcattgtcac ttgtatagat acctgaccaa tctgggccgc ggtgacgtag taactttgat 11220

agttctagtg cttgttcgcg aagaggttta atgtctgatt tgatgtctag aattccgaat 11280

attgagcaca taactaattc cttctggggc tgcgtctgca gctaactttc taaatagtgt 11340

gtctaatttg ccacattgta gatttaatgc aaacattaat gataaaacat ttataaaaaa 11400

tgtaattcaa tgtggaatcg ataatttaat ggcttaaaag tgaagatcca ttaattgtga 11460

tggcgaggtg atagaccaat gtagacctta atgaataaag caggcacgat tgaatccatt 11520

caacgcaaag tggtactaac tattgtttta aacgttataa atagtgtttt aaaggttata 11580

agtaaataat ttaaaaacaa taataatcca catgcattaa atttatcatg ataaaccgct 11640

atatctcaat ggcaatttgg gataagtgta aaatatatgt aaaatgaatg agttgacttg 11700

ctttttttac actaagtgat gaaattaaag ctagatgtcg ttgttagcat tgattaataa 11760

cgtactaaaa tacgacatct agtatagaaa tttaaaaaac agttggtttt gatagcataa 11820

ctgcataaac taatcagctt attgtctgta atatttttgt aatttaaata ggtttaataa 11880

aattatatgt ctgataaata taaaccgtac gacctttcct ttaaaaagac gtttttgctg 11940

cctaagtttt ggcctgtgtg gttcggggtg tttgcaatat acttattagc ttttatgcca 12000

gtaaagccgc gtgataaatt tgctcgattc atagcgaaga aattgtttag tctaaaaatg 12060

atggcaaagc gtaaaaaggt agcaaagatc aatttatcta tgtgcttccc tgaaatggat 12120

gatacggaac aagaccgtat aatcatggtc aatctagtta ctttttgtca aactatctta 12180

agttatgcag agccaagtgc gcgtagtcgt gcttataacc gtgaccgtat gatagtgcat 12240

ggtggcgaga atttatttcc gctacttgaa caaggtaagg cttgtatctt attagtgccg 12300

catagcttcg ctattgattt tgcaggttta cacattgctt cttatggcgc gccattttgt 12360

actatgttta acaattctga gaatgagttg ttcgattggc tgatgacacg tcaacgcgct 12420

atgtttggag gcactgttta tcaccgcaag gcagggctag gggctctagt taaatcactt 12480

aagagcggtg aaagctgtta ttacttacct gatgaagacc atggacctaa gcgtagtgta 12540

tttgcgcctt tatttgcgac tcaaaaagca actttacctg taatgggcaa gctagcagaa 12600

aaaacaaatg cactcgttgt tcctgtttat gcggcatata atgaatcact aggtaaattt 12660

gaaaccttta ttcgaccagc aatgcaaaac tttccatcag aaagcccaga acaagatgca 12720

gtgatgatga ataaagagat tgaagccttg attgaatgtg gtgttgatca atatatgtgg 12780

acacttagat tattgagaac acgtccggac ggtaaaaaaa tctactaata aagtttaata 12840

aacaccataa tcttcgttga atatggtgtt tacccccctg aataccctct aaattaataa 12900

caaaaaaagc catttacgta acatctaatg atgatttagc ctgcacttgc tttgttttta 12960

gtcttaagag cctaataaac ttgatctagg tatagattct gtctttcttt acgtaacgcg 13020

atctattttt tttaaccgat agttgttata attagtttca tatgaaagag atatcgtttc 13080

agtaaaagct atttcgtttc aatagataat ttatttatag tcatattttc tgtaatgaca 13140

atcattttct catctagact atagataaga atacgaatta agtaagaaca ttaattttac 13200

aagaatataa aatatcccat cggagctata agaatgaaaa agactaaaat tgtttgtaca 13260

attggtccaa aaactgaatc agtagagaaa ctaacagagc ttgttaatgc aggcatgaac 13320

gttatgcgtt taaatttctc tcatggtaac tttgctgaac attcagtgcg tattcaaaat 13380

atccgtcaag taagtgaaaa cctgaataag aaaattgctg ttttactgga tactaaaggt 13440

ccagaaatcc gtacgattaa actagaaaac ggtgacgatg taatgttgac cgctggtcag 13500

tcattcacgt ttacaacaga cattaacgtg gtaggtaata aagactgtgt tgctgtaaca 13560

tatgctggtt ttgctaaaga ccttaatcct ggtgcaatca tccttgttga tgatggttta 13620

attgaaatgg aagttgttgc aacaactgac actgaagtta aatgtacagt attaaatact 13680

ggtgcacttg gtgaaaataa aggcgttaac ttacctaaca tcagtgtagg tctacctgca 13740

ttgtcagaaa aagataaagc tgatttagcg tttggttgtg agcaagaagt tgattttgtt 13800

gctgcatcat ttattcgtaa ggctgatgat gtaagagaaa ttcgtgaaat cctatttaat 13860

aatggtggcg aaaacattca gattatctcg aaaattgaaa accaagaagg tgtagacaat 13920

ttcgatgaaa tcttagctga atcagacggt atcatggttg ctcgtggcga tctcggtgtt 13980

gagatcccag ttgaagaagt gatcatggca cagaagatga tgatcaaaaa atgtaataaa 14040

gcaggtaaag ttgtaattac tgcaacacaa atgcttgatt caatgatcag taacccacgt 14100

ccaacacgtg cagaagcggg cgatgttgcc aatgctgtgc ttgacggtac cgacgcggta 14160

atgctttctg gtgaaactgc gaaaggtaaa tacccagttg aagctgtgtc tatcatggca 14220

aacatctgtg aacgtactga taactcaatg tcttcggatt taggtgcgaa cattgttgct 14280

aaaagcatgc gcattacaga agctgtgtgt aaaggtgcgg tagaaacaac agaaaaattg 14340

tgtgctccac ttattgttgt tgcaactcgt ggcggtaaat cagcaaaatc tgttcgtaaa 14400

tacttcccga aagcaaatat tcttgctatc acaacaaatg aaaaagcagc gcaacagtta 14460

tgcctaacta aaggcgtaag cagctgcatc gttgagcaga ttgatagcac tgatgagttc 14520

taccgtaaag gtaaagagct tgcattagca actggtttag ctaaagaagg cgatatcgtt 14580

gttatggtat caggtgcgtt agtaccatca ggtacaacga atacggcatc tgttcaccaa 14640

ctttaagttg ccatattgat attataaaaa agagagcgta tgctctcttt ttttatatct 14700

gtagtttata tgtctgtaca aaaaaatgat aaagagtaca taaactatta atatagcgta 14760

atatataatg attaacggtg atgaaagggt taaataaatg gatagtgcta aacataaaat 14820

tggcttagtc ctttctggcg gtggtgcgaa aggtattgct catcttggtg tattaaaata 14880

cctgttagag caagatataa gaccgaatgt aattgcgggt acaagtgctg gctctatggt 14940

tggtgcactt tattgctcag gacttgagat tgatgacatt ttacaattct tcatcgatgt 15000

aaaacctttt tcttggaagt ttacccgtgc ccgtgctggc tttatagacc cggcaaaatt 15060

atatcctgaa gtgctaaaat atatccccga ggatagcttt gagtaccttc aacctgaatt 15120

gcgcattgtt gccaccaaca tgttactcgg taaagagcat atatttaaag atggctccgt 15180

gattaatgcc ttattagcat cagccagcta ccctttagtt ttttctccga tgatcattga 15240

cgatcaagtg tattcagatg gcggtattgt taatcatttc cccgtgagtg tcattgaaga 15300

tgattgcgat aaaataatcg gcgtatacgt gtcgcccatt cgtcaggtcg aagctgacga 15360

actctcgagt ataaaagacg tggtattacg tgcgttcacg ctgcagggta gtggtgctga 15420

attagataaa ctatcgcaat gtgatgtgca aatttatcca gaagcgctat tgaattacaa 15480

tacgtttgca accgatgaaa aatcattacg ggagatctac cagattggtt atgatgctgc 15540

aaaagatcaa catgacaacc ttatggcatt gaaagaaagt atcaccacca gcgaggttaa 15600

aaagaacgtc tttagcaaat ggtttggtga taaacttgct agcaacagcg gcaaatagcg 15660

gcccacacgg atttatacac taggataatg ggcgttaata gcctcactgt cgttgtgtgg 15720

tctctaattt tagctaaatc ttgtgttata ctgacttcct attaatcata aacgatttat 15780

cacggtaaac atgactcaaa taaataaccc gcttcacggc atgacactcg aaaaagtaat 15840

taacagtctc gttgaacaat atggctggga tggtcttgga tactacatca acattcgttg 15900

ctttactgaa aatccaagtg ttaagtctag tcttaaattt ttacgtaaaa ccccttgggc 15960

acgtgataaa gtagaagcgc tatatatcaa aatggtgact gaaggctaac tgtctccacg 16020

ctagcgaacc gctgtttata gttaatataa gtactataag cagggctcgt taattcagta 16080

tgtaattaat cctgaatacc tccgcttatt tcaacattgt actctctaga taacactctc 16140

aacattacac cttcaacatc acagcctcca cataacatcc gatgacatag ccctgttatt 16200

tttcacattt atctatatgc tatatatttt agccatttga tcaattgagt taatttctgc 16260

aatgacaaag atataccatc atccagtaca aatttattat gaagataccg accattctgg 16320

tgttgtttac caccctaact ttttaaaata ctttgaacgt gcacgtgagc atgtgataaa 16380

tagtgactta ctagcaacat tgtggaatga acgcggttta ggttttgcgg tgtataaagc 16440

caatatgact tttcaggatg gggtcgaatt tgctgaagtg tgtgatattc gcacttcttt 16500

tgtcctagac ggtaagtaca aaacgatctg gcgccaagaa gtatggcgtc cgaatgcgac 16560

tagggctgcc gttatcggtg atattgaaat ggtgtgctta gacaaacaaa aacgtttaca 16620

gcccatccct gatgatgtgt tagctgcaat ggttagtgaa taaatggttc atgcataaat 16680

agttaataca tgattctggc ccgtcacgtt tacagataag aggcatccga tgcctccttc 16740

ctattaccaa tactactgct tatccctttc taactatctt tagcgtccat aacacactga 16800

gcatttattc tattaatcag tgattgtgat ttaattatct tctatatatg taatttaatg 16860

taattttcaa tttattttta gctacattaa ggcttacgaa tgtacgctaa aatgagatgt 16920

cagactaatt ttagcttatt aatctgttag ccgtttatat tttataaaga tgggatttaa 16980

cttaaatgca attaattatg gcgtaaatag agtgaaaaca tggctaatat tcactaagtc 17040

ctgaatttta tataaagttt aatctgttat tttagcgttt acctggtctt atcagtgagg 17100

tttatagcca ttattagtgg gattgaagtg atttttaaag ctatgtatat tattgcaaat 17160

ataaattgta acaattaaga ctttggacac ttgagttcaa tttcgaattg attggcataa 17220

aatttaaaac agctaaatct acctcaatca ttttagcaaa tgtatgcagg tagatttttt 17280

tcgccattta agagtacact tgtacgctag gtttttgttt agtgtgcaaa tgaacgtttt 17340

gatgagcatt gtttttagag cacaaaatag atccttacag gagcaataac gcaatggcta 17400

aaaagaacac cacatcgatt aagcacgcca aggatgtgtt aagtagtgat gatcaacagt 17460

taaattctcg cttgcaagaa tgtccgattg ccatcattgg tatggcatcg gtttttgcag 17520

atgctaaaaa cttggatcaa ttctgggata acatcgttga ctctgtggac gctattattg 17580

atgtgcctag cgatcgctgg aacattgacg accattactc ggctgataaa aaagcagctg 17640

acaagacata ctgcaaacgc ggtggtttca ttccagagct tgattttgat ccgatggagt 17700

ttggtttacc gccaaatatc ctcgagttaa ctgacatcgc tcaattgttg tcattaattg 17760

ttgctcgtga tgtattaagt gatgctggca ttggtagtga ttatgaccat gataaaattg 17820

gtatcacgct gggtgtcggt ggtggtcaga aacaaatttc gccattaacg tcgcgcctac 17880

aaggcccggt attagaaaaa gtattaaaag cctcaggcat tgatgaagat gatcgcgcta 17940

tgatcatcga caaatttaaa aaagcctaca tcggctggga agagaactca ttcccaggca 18000

tgctaggtaa cgttattgct ggtcgtatcg ccaatcgttt tgattttggt ggtactaact 18060

gtgtggttga tgcggcatgc gctggctccc ttgcagctgt taaaatggcg atctcagact 18120

tacttgaata tcgttcagaa gtcatgatat cgggtggtgt atgttgtgat aactcgccat 18180

tcatgtatat gtcattctcg aaaacaccag catttaccac caatgatgat atccgtccgt 18240

ttgatgacga ttcaaaaggc atgctggttg gtgaaggtat tggcatgatg gcgtttaaac 18300

gtcttgaaga tgctgaacgt gacggcgaca aaatttattc tgtactgaaa ggtatcggta 18360

catcttcaga tggtcgtttc aaatctattt acgctccacg cccagatggc caagcaaaag 18420

cgctaaaacg tgcttatgaa gatgccggtt ttgcccctga aacatgtggt ctaattgaag 18480

gccatggtac gggtaccaaa gcgggtgatg ccgcagaatt tgctggcttg accaaacact 18540

ttggcgccgc cagtgatgaa aagcaatata tcgccttagg ctcagttaaa tcgcaaattg 18600

gtcatactaa atctgcggct ggctctgcgg gtatgattaa ggcggcatta gcgctgcatc 18660

ataaaatctt acctgcaacg atccatatcg ataaaccaag tgaagccttg gatatcaaaa 18720

acagcccgtt atacctaaac agcgaaacgc gtccttggat gccacgtgaa gatggtattc 18780

cacgtcgtgc aggtatcagc tcatttggtt ttggcggcac caacttccat attattttag 18840

aagagtatcg cccaggtcac gatagcgcat atcgcttaaa ctcagtgagc caaactgtgt 18900

tgatctcggc aaacgaccaa caaggtattg ttgctgagtt aaataactgg cgtactaaac 18960

tggctgtcga tgctgatcat caagggtttg tatttaatga gttagtgaca acgtggccat 19020

taaaaacccc atccgttaac caagctcgtt taggttttgt tgcgcgtaat gcaaatgaag 19080

cgatcgcgat gattgatacg gcattgaaac aattcaatgc gaacgcagat aaaatgacat 19140

ggtcagtacc taccggggtt tactatcgtc aagccggtat tgatgcaaca ggtaaagtgg 19200

ttgcgctatt ctcagggcaa ggttcgcaat acgtgaacat gggtcgtgaa ttaacctgta 19260

acttcccaag catgatgcac agtgctgcgg cgatggataa agagttcagt gccgctggtt 19320

taggccagtt atctgcagtt actttcccta tccctgttta tacggatgcc gagcgtaagc 19380

tacaagaaga gcaattacgt ttaacgcaac atgcgcaacc agcgattggt agtttgagtg 19440

ttggtctgtt caaaacgttt aagcaagcag gttttaaagc tgattttgct gccggtcata 19500

gtttcggtga gttaaccgca ttatgggctg ccgatgtatt gagcgaaagc gattacatga 19560

tgttagcgcg tagtcgtggt caagcaatgg ctgcgccaga gcaacaagat tttgatgcag 19620

gtaagatggc cgctgttgtt ggtgatccaa agcaagtcgc tgtgatcatt gatacccttg 19680

atgatgtctc tattgctaac ttcaactcga ataaccaagt tgttattgct ggtactacgg 19740

agcaggttgc tgtagcggtt acaaccttag gtaatgctgg tttcaaagtt gtgccactgc 19800

cggtatctgc tgcgttccat acacctttag ttcgtcacgc gcaaaaacca tttgctaaag 19860

cggttgatag cgctaaattt aaagcgccaa gcattccagt gtttgctaat ggcacaggct 19920

tggtgcattc aagcaaaccg aatgacatta agaaaaacct gaaaaaccac atgctggaat 19980

ctgttcattt caatcaagaa attgacaaca tctatgctga tggtggccgc gtatttatcg 20040

aatttggtcc aaagaatgta ttaactaaat tggttgaaaa cattctcact gaaaaatctg 20100

atgtgactgc tatcgcggtt aatgctaatc ctaaacaacc tgcggacgta caaatgcgcc 20160

aagctgcgct gcaaatggca gtgcttggtg tcgcattaga caatattgac ccgtacgacg 20220

ccgttaagcg tccacttgtt gcgccgaaag catcaccaat gttgatgaag ttatctgcag 20280

cgtcttatgt tagtccgaaa acgaagaaag cgtttgctga tgcattgact gatggctgga 20340

ctgttaagca agcgaaagct gtacctgctg ttgtgtcaca accacaagtg attgaaaaga 20400

tcgttgaagt tgaaaagata gttgaacgca ttgtcgaagt agagcgtatt gtcgaagtag 20460

aaaaaatcgt ctacgttaat gctgacggtt cgcttatatc gcaaaataat caagacgtta 20520

acagcgctgt tgttagcaac gtgactaata gctcagtgac tcatagcagt gatgctgacc 20580

ttgttgcctc tattgaacgc agtgttggtc aatttgttgc acaccaacag caattattaa 20640

atgtacatga acagtttatg caaggtccac aagactacgc gaaaacagtg cagaacgtac 20700

ttgctgcgca gacgagcaat gaattaccgg aaagtttaga ccgtacattg tctatgtata 20760

acgagttcca atcagaaacg ctacgtgtac atgaaacgta cctgaacaat cagacgagca 20820

acatgaacac catgcttact ggtgctgaag ctgatgtgct agcaacccca ataactcagg 20880

tagtgaatac agccgttgcc actagtcaca aggtagttgc tccagttatt gctaatacag 20940

tgacgaatgt tgtatctagt gtcagtaata acgcggcggt tgcagtgcaa actgtggcat 21000

tagcgcctac gcaagaaatc gctccaacag tcgctactac gccagcaccc gcattggttg 21060

ctatcgtggc tgaacctgtg attgttgcgc atgttgctac agaagttgca ccaattacac 21120

catcagttac accagttgtc gcaactcaag cggctatcga tgtagcaact attaacaaag 21180

taatgttaga agttgttgct gataaaaccg gttatccaac ggatatgctg gaactgagca 21240

tggacatgga agctgactta ggtatcgact caatcaaacg tgttgagata ttaggcgcag 21300

tacaggaatt gatccctgac ttacctgaac ttaatcctga agatcttgct gagctacgca 21360

cgcttggtga gattgtcgat tacatgaatt caaaagccca ggctgtagct cctacaacag 21420

tacctgtaac aagtgcacct gtttcgcctg catctgctgg tattgattta gcccacatcc 21480

aaaacgtaat gttagaagtg gttgcagaca aaaccggtta cccaacagac atgctagaac 21540

tgagcatgga tatggaagct gacttaggta ttgattcaat caagcgtgtg gaaatcttag 21600

gtgcagtaca ggagatcata actgatttac ctgagctaaa ccctgaagat cttgctgaat 21660

tacgcaccct aggtgaaatc gttagttaca tgcaaagcaa agcgccagtc gctgaaagtg 21720

cgccagtggc gacggctcct gtagcaacaa gctcagcacc gtctatcgat ttgaaccaca 21780

ttcaaacagt gatgatggat gtagttgcag ataagactgg ttatccaact gacatgctag 21840

aacttggcat ggacatggaa gctgatttag gtatcgattc aatcaaacgt gtggaaatat 21900

taggcgcagt gcaggagatc atcactgatt tacctgagct aaacccagaa gacctcgctg 21960

aattacgcac gctaggtgaa atcgttagtt acatgcaaag caaagcgcca gtcgctgaga 22020

gtgcgccagt agcgacggct tctgtagcaa caagctctgc accgtctatc gatttaaacc 22080

atatccaaac agtgatgatg gaagtggttg cagacaaaac cggttatcca gtagacatgt 22140

tagaacttgc tatggacatg gaagctgacc taggtatcga ttcaatcaag cgtgtagaaa 22200

ttttaggtgc ggtacaggaa atcattactg acttacctga gcttaaccct gaagatcttg 22260

ctgaactacg tacattaggt gaaatcgtta gttacatgca aagcaaagcg cccgtagctg 22320

aagcgcctgc agtacctgtt gcagtagaaa gtgcacctac tagtgtaaca agctcagcac 22380

cgtctatcga tttagaccac atccaaaatg taatgatgga tgttgttgct gataagactg 22440

gttatcctgc caatatgctt gaattagcaa tggacatgga agccgacctt ggtattgatt 22500

caatcaagcg tgttgaaatt ctaggcgcgg tacaggagat cattactgat ttacctgaac 22560

taaacccaga agacttagct gaactacgta cgttagaaga aattgtaacc tacatgcaaa 22620

gcaaggcgag tggtgttact gtaaatgtag tggctagccc tgaaaataat gctgtatcag 22680

atgcatttat gcaaagcaat gtggcgacta tcacagcggc cgcagaacat aaggcggaat 22740

ttaaaccggc gccgagcgca accgttgcta tctctcgtct aagctctatc agtaaaataa 22800

gccaagattg taaaggtgct aacgccttaa tcgtagctga tggcactgat aatgctgtgt 22860

tacttgcaga ccacctattg caaactggct ggaatgtaac tgcattgcaa ccaacttggg 22920

tagctgtaac aacgacgaaa gcatttaata agtcagtgaa cctggtgact ttaaatggcg 22980

ttgatgaaac tgaaatcaac aacattatta ctgctaacgc acaattggat gcagttatct 23040

atctgcacgc aagtagcgaa attaatgcta tcgaataccc acaagcatct aagcaaggcc 23100

tgatgttagc cttcttatta gcgaaattga gtaaagtaac tcaagccgct aaagtgcgtg 23160

gcgcctttat gattgttact cagcagggtg gttcattagg ttttgatgat atcgattctg 23220

ctacaagtca tgatgtgaaa acagacctag tacaaagcgg cttaaacggt ttagttaaga 23280

cactgtctca cgagtgggat aacgtattct gtcgtgcggt tgatattgct tcgtcattaa 23340

cggctgaaca agttgcaagc cttgttagtg atgaactact tgatgctaac actgtattaa 23400

cagaagtggg ttatcaacaa gctggtaaag gccttgaacg tatcacgtta actggtgtgg 23460

ctactgacag ctatgcatta acagctggca ataacatcga tgctaactcg gtatttttag 23520

tgagtggtgg cgcaaaaggt gtaactgcac attgtgttgc tcgtatagct aaagaatatc 23580

agtctaagtt catcttattg ggacgttcaa cgttctcaag tgacgaaccg agctgggcaa 23640

gtggtattac tgatgaagcg gcgttaaaga aagcagcgat gcagtctttg attacagcag 23700

gtgataaacc aacacccgtt aagatcgtac agctaatcaa accaatccaa gctaatcgtg 23760

aaattgcgca aaccttgtct gcaattaccg ctgctggtgg ccaagctgaa tatgtttctg 23820

cagatgtaac taatgcagca agcgtacaaa tggcagtcgc tccagctatc gctaagttcg 23880

gtgcaatcac tggcatcatt catggcgcgg gtgtgttagc tgaccaattc attgagcaaa 23940

aaacactgag tgattttgag tctgtttaca gcactaaaat tgacggtttg ttatcgctac 24000

tatcagtcac tgaagcaagc aacatcaagc aattggtatt gttctcgtca gcggctggtt 24060

tctacggtaa ccccggccag tctgattact cgattgccaa tgagatctta aataaaaccg 24120

cataccgctt taaatcattg cacccacaag ctcaagtatt gagctttaac tggggtcctt 24180

gggacggtgg catggtaacg cctgagctta aacgtatgtt tgaccaacgt ggtgtttaca 24240

ttattccact tgatgcaggt gcacagttat tgctgaatga actagccgct aatgataacc 24300

gttgtccaca aatcctcgtg ggtaatgact tatctaaaga tgctagctct gatcaaaagt 24360

ctgatgaaaa gagtactgct gtaaaaaagc cacaagttag tcgtttatca gatgctttag 24420

taactaaaag tatcaaagcg actaacagta gctctttatc aaacaagact agtgctttat 24480

cagacagtag tgcttttcag gttaacgaaa accacttttt agctgaccac atgatcaaag 24540

gcaatcaggt attaccaacg gtatgcgcga ttgcttggat gagtgatgca gcaaaagcga 24600

cttatagtaa ccgagactgt gcattgaagt atgtcggttt cgaagactat aaattgttta 24660

aaggtgtggt ttttgatggc aatgaggcgg cggattacca aatccaattg tcgcctgtga 24720

caagggcgtc agaacaggat tctgaagtcc gtattgccgc aaagatcttt agcctgaaaa 24780

gtgacggtaa acctgtgttt cattatgcag cgacaatatt gttagcaact cagccactta 24840

atgctgtgaa ggtagaactt ccgacattga cagaaagtgt tgatagcaac aataaagtaa 24900

ctgatgaagc acaagcgtta tacagcaatg gcaccttgtt ccacggtgaa agtctgcagg 24960

gcattaagca gatattaagt tgtgacgaca agggcctgct attggcttgt cagataaccg 25020

atgttgcaac agctaagcag ggatccttcc cgttagctga caacaatatc tttgccaatg 25080

atttggttta tcaggctatg ttggtctggg tgcgcaaaca atttggttta ggtagcttac 25140

cttcggtgac aacggcttgg actgtgtatc gtgaagtggt tgtagatgaa gtattttatc 25200

tgcaacttaa tgttgttgag catgatctat tgggttcacg cggcagtaaa gcccgttgtg 25260

atattcaatt gattgctgct gatatgcaat tacttgccga agtgaaatca gcgcaagtca 25320

gtgtcagtga cattttgaac gatatgtcat gatcgagtaa ataataacga taggcgtcat 25380

ggtgagcatg gcgtctgctt tcttcatttt ttaacattaa caatattaat agctaaacgc 25440

ggttgcttta aaccaagtaa acaagtgctt ttagctatta ctattccaaa caggatatta 25500

aagagaatat gacggaatta gctgttattg gtatggatgc taaatttagc ggacaagaca 25560

atattgaccg tgtggaacgc gctttctatg aaggtgctta tgtaggtaat gttagccgcg 25620

ttagtaccga atctaatgtt attagcaatg gcgaagaaca agttattact gccatgacag 25680

ttcttaactc tgtcagtcta ctagcgcaaa cgaatcagtt aaatatagct gatatcgcgg 25740

tgttgctgat tgctgatgta aaaagtgctg atgatcagct tgtagtccaa attgcatcag 25800

caattgaaaa acagtgtgcg agttgtgttg ttattgctga tttaggccaa gcattaaatc 25860

aagtagctga tttagttaat aaccaagact gtcctgtggc tgtaattggc atgaataact 25920

cggttaattt atctcgtcat gatcttgaat ctgtaactgc aacaatcagc tttgatgaaa 25980

ccttcaatgg ttataacaat gtagctgggt tcgcgagttt acttatcgct tcaactgcgt 26040

ttgccaatgc taagcaatgt tatatatacg ccaacattaa gggcttcgct caatcgggcg 26100

taaatgctca atttaacgtt ggaaacatta gcgatactgc aaagaccgca ttgcagcaag 26160

ctagcataac tgcagagcag gttggtttgt tagaagtgtc agcagtcgct gattcggcaa 26220

tcgcattgtc tgaaagccaa ggtttaatgt ctgcttatca tcatacgcaa actttgcata 26280

ctgcattaag cagtgcccgt agtgtgactg gtgaaggcgg gtgtttttca caggtcgcag 26340

gtttattgaa atgtgtaatt ggtttacatc aacgttatat tccggcgatt aaagattggc 26400

aacaaccgag tgacaatcaa atgtcacggt ggcggaattc accattctat atgcctgtag 26460

atgctcgacc ttggttccca catgctgatg gctctgcaca cattgccgct tatagttgtg 26520

tgactgctga cagctattgt catattcttt tacaagaaaa cgtcttacaa gaacttgttt 26580

tgaaagaaac agtcttgcaa gataatgact taactgaaag caagcttcag actcttgaac 26640

aaaacaatcc agtagctgat ctgcgcacta atggttactt tgcatcgagc gagttagcat 26700

taatcatagt acaaggtaat gacgaagcac aattacgctg tgaattagaa actattacag 26760

ggcagttaag tactactggc ataagtacta tcagtattaa acagatcgca gcagactgtt 26820

atgcccgtaa tgatactaac aaagcctata gcgcagtgct tattgccgag actgctgaag 26880

agttaagcaa agaaataacc ttggcgtttg ctggtatcgc tagcgtgttt aatgaagatg 26940

ctaaagaatg gaaaaccccg aagggcagtt attttaccgc gcagcctgca aataaacagg 27000

ctgctaacag cacacagaat ggtgtcacct tcatgtaccc aggtattggt gctacatatg 27060

ttggtttagg gcgtgatcta tttcatctat tcccacagat ttatcagcct gtagcggctt 27120

tagccgatga cattggcgaa agtctaaaag atactttact taatccacgc agtattagtc 27180

gtcatagctt taaagaactc aagcagttgg atctggacct gcgcggtaac ttagccaata 27240

tcgctgaagc cggtgtgggt tttgcttgtg tgtttaccaa ggtatttgaa gaagtctttg 27300

ccgttaaagc tgactttgct acaggttata gcatgggtga agtaagcatg tatgcagcac 27360

taggctgctg gcagcaaccg ggattgatga gtgctcgcct tgcacaatcg aataccttta 27420

atcatcaact ttgcggcgag ttaagaacac tacgtcagca ttggggcatg gatgatgtag 27480

ctaacggtac gttcgagcag atctgggaaa cctataccat taaggcaacg attgaacagg 27540

tcgaaattgc ctctgcagat gaagatcgtg tgtattgcac cattatcaat acacctgata 27600

gcttgttgtt agccggttat ccagaagcct gtcagcgagt cattaagaat ttaggtgtgc 27660

gtgcaatggc attgaatatg gcgaacgcaa ttcacagcgc gccagcttat gccgaatacg 27720

atcatatggt tgagctatac catatggatg ttactccacg tattaatacc aagatgtatt 27780

caagctcatg ttatttaccg attccacaac gcagcaaagc gatttcccac agtattgcta 27840

aatgtttgtg tgatgtggtg gatttcccac gtttggttaa taccttacat gacaaaggtg 27900

cgcgggtatt cattgaaatg ggtccaggtc gttcgttatg tagctgggta gataagatct 27960

tagttaatgg cgatggcgat aataaaaagc aaagccaaca tgtatctgtt cctgtgaatg 28020

ccaaaggcac cagtgatgaa cttacttata ttcgtgcgat tgctaagtta attagtcatg 28080

gcgtgaattt gaatttagat agcttgttta acgggtcaat cctggttaaa gcaggccata 28140

tagcaaacac gaacaaatag tcaacatcga tatctagcgc tggtgagtta tacctcatta 28200

gttgaaatat ggatttaaag agagtaatta tggaaaatat tgcagtagta ggtattgcta 28260

atttgttccc gggctcacaa gcaccggatc aattttggca gcaattgctt gaacaacaag 28320

attgccgcag taaggcgacc gctgttcaaa tgggcgttga tcctgctaaa tataccgcca 28380

acaaaggtga cacagataaa ttttactgtg tgcacggcgg ttacatcagt gatttcaatt 28440

ttgatgcttc aggttatcaa ctcgataatg attatttagc cggtttagat gaccttaatc 28500

aatgggggct ttatgttacg aaacaagccc ttaccgatgc gggttattgg ggcagtactg 28560

cactagaaaa ctgtggtgtg attttaggta atttgtcatt cccaactaaa tcatctaatc 28620

agctgtttat gcctttgtat catcaagttg ttgataatgc cttaaaggcg gtattacatc 28680

ctgattttca attaacgcat tacacagcac cgaaaaaaac acatgctgac aatgcattag 28740

tagcaggtta tccagctgca ttgatcgcgc aagcggcggg tcttggtggt tcacattttg 28800

cactggatgc ggcttgtgct tcatcttgtt atagcgttaa gttagcgtgt gattacctgc 28860

atacgggtaa agccaacatg atgcttgctg gtgcggtatc tgcagcagat cctatgttcg 28920

taaatatggg tttctcgata ttccaagctt acccagctaa caatgtacat gccccgtttg 28980

accaaaattc acaaggtcta tttgccggtg aaggcgcggg catgatggta ttgaaacgtc 29040

aaagtgatgc agtacgtgat ggtgatcata tttacgccat tattaaaggc ggcgcattat 29100

cgaatgacgg taaaggcgag tttgtattaa gcccgaacac caagggccaa gtattagtat 29160

atgaacgtgc ttatgccgat gcagatgttg acccgagtac agttgactat attgaatgtc 29220

atgcaacggg cacacctaag ggtgacaatg ttgaattgcg ttcgatggaa acctttttca 29280

gtcgcgtaaa taacaaacca ttactgggct cggttaaatc taaccttggt catttgttaa 29340

ctgccgctgg tatgcctggc atgaccaaag ctatgttagc gctaggtaaa ggtcttattc 29400

ctgcaacgat taacttaaag caaccactgc aatctaaaaa cggttacttt actggcgagc 29460

aaatgccaac gacgactgtg tcttggccaa caactccggg tgccaaggca gataaaccgc 29520

gtaccgcagg tgtgagcgta tttggttttg gtggcagcaa cgcccatttg gtattacaac 29580

agccaacgca aacactcgag actaatttta gtgttgctaa accacgtgag cctttggcta 29640

ttattggtat ggacagccat tttggtagtg ccagtaattt agcgcagttc aaaaccttat 29700

taaataataa tcaaaatacc ttccgtgaat taccagaaca acgctggaaa ggcatggaaa 29760

gtaacgctaa cgtcatgcag tcgttacaat tacgcaaagc gcctaaaggc agttacgttg 29820

aacagctaga tattgatttc ttgcgtttta aagtaccgcc taatgaaaaa gattgcttga 29880

tcccgcaaca gttaatgatg atgcaagtgg cagacaatgc tgcgaaagac ggaggtctag 29940

ttgaaggtcg taatgttgcg gtattagtag cgatgggcat ggaactggaa ttacatcagt 30000

atcgtggtcg cgttaatcta accacccaaa ttgaagacag cttattacag caaggtatta 30060

acctgactgt tgagcaacgt gaagaactga ccaatattgc taaagacggt gttgcctcgg 30120

ctgcacagct aaatcagtat acgagtttca ttggtaatat tatggcgtca cgtatttcgg 30180

cgttatggga tttttctggt cctgctatta ccgtatcggc tgaagaaaac tctgtttatc 30240

gttgtgttga attagctgaa aatctatttc aaaccagtga tgttgaagcc gttattattg 30300

ctgctgttga tttgtctggt tcaattgaaa acattacttt acgtcagcac tacggtccag 30360

ttaatgaaaa gggatctgta agtgaatgtg gtccggttaa tgaaagcagt tcagtaacca 30420

acaatattct tgatcagcaa caatggctgg tgggtgaagg cgcagcggct attgtcgtta 30480

aaccgtcatc gcaagtcact gctgagcaag tttatgcgcg tattgatgcg gtgagttttg 30540

cccctggtag caatgcgaaa gcaattacga ttgcagcgga taaagcatta acacttgctg 30600

gtatcagtgc tgctgatgta gctagtgttg aagcacatgc aagtggtttt agtgccgaaa 30660

ataatgctga aaaaaccgcg ttaccgactt tatacccaag cgcaagtatc agttcggtga 30720

aagccaatat tggtcatacg tttaatgcct cgggtatggc gagtattatt aaaacggcgc 30780

tgctgttaga tcagaatacg agtcaagatc agaaaagcaa acatattgct attaacggtc 30840

taggtcgtga taacagctgc gcgcatctta tcttatcgag ttcagcgcaa gcgcatcaag 30900

ttgcaccagc gcctgtatct ggtatggcca agcaacgccc acagttagtt aaaaccatca 30960

aactcggtgg tcagttaatt agcaacgcga ttgttaacag tgcgagttca tctttacacg 31020

ctattaaagc gcagtttgcc ggtaagcact taaacaaagt taaccagcca gtgatgatgg 31080

ataacctgaa gccccaaggt attagcgctc atgcaaccaa tgagtatgtg gtgactggag 31140

ctgctaacac tcaagcttct aacattcaag catctcatgt tcaagcgtca agtcatgcac 31200

aagagatagc accaaaccaa gttcaaaata tgcaagctac agcagccgct gtaagttcac 31260

ccctttctca acatcaacac acagcgcagc ccgtagcggc accgagcgtt gttggagtga 31320

ctgtgaaaca taaagcaagt aaccaaattc atcagcaagc gtctacgcat aaagcatttt 31380

tagaaagtcg tttagctgca cagaaaaacc tatcgcaact tgttgaattg caaaccaagc 31440

tgtcaatcca aactggtagt gacaatacat ctaacaatac tgcgtcaaca agcaatacag 31500

tgctaacaaa tcctgtatca gcaacgccat taacacttgt gtctaatgcg cctgtagtag 31560

cgacaaacct aaccagtaca gaagcaaaag cgcaagcagc tgctacacaa gctggttttc 31620

agataaaagg acctgttggt tacaactatc caccgctgca gttaattgaa cgttataata 31680

aaccagaaaa cgtgatttac gatcaagctg atttggttga attcgctgaa ggtgatattg 31740

gtaaggtatt tggtgctgaa tacaatatta ttgatggcta ttcgcgtcgt gtacgtctgc 31800

caacctcaga ttacttgtta gtaacacgtg ttactgaact tgatgccaag gtgcatgaat 31860

acaagaaatc atacatgtgt actgaatatg atgtgcctgt tgatgcaccg ttcttaattg 31920

atggtcagat cccttggtct gttgccgtcg aatcaggcca gtgtgatttg atgttgattt 31980

catatatcgg tattgatttc caagcgaaag gcgaacgtgt ttaccgttta cttgattgtg 32040

aattaacttt ccttgaagag atggcttttg gtggcgatac tttacgttac gagatccaca 32100

ttgattcgta tgcacgtaac ggcgagcaat tattattctt cttccattac gattgttacg 32160

taggggataa gaaggtactt atcatgcgta atggttgtgc tggtttcttt actgacgaag 32220

aactttctga tggtaaaggc gttattcata acgacaaaga caaagctgag tttagcaatg 32280

ctgttaaatc atcattcacg ccgttattac aacataaccg tggtcaatac gattataacg 32340

acatgatgaa gttggttaat ggtgatgttg ccagttgttt tggtccgcaa tatgatcaag 32400

gtggccgtaa tccatcattg aaattctcgt ctgagaagtt cttgatgatt gaacgtatta 32460

ccaagataga cccaaccggt ggtcattggg gactaggcct gttagaaggt cagaaagatt 32520

tagaccctga gcattggtat ttcccttgtc actttaaagg tgatcaagta atggctggtt 32580

cgttgatgtc ggaaggttgt ggccaaatgg cgatgttctt catgctgtct cttggtatgc 32640

ataccaatgt gaacaacgct cgtttccaac cactaccagg tgaatcacaa acggtacgtt 32700

gtcgtgggca agtactgcca cagcgcaata ccttaactta ccgtatggaa gttactgcga 32760

tgggtatgca tccacagcca ttcatgaaag ctaatattga tattttgctt gacggtaaag 32820

tggttgttga tttcaaaaac ttgagcgtga tgatcagcga acaagatgag cattcagatt 32880

accctgtaac actgccgagt aatgtggcgc ttaaagcgat tactgcacct gttgcgtcag 32940

tagcaccagc atcttcaccc gctaacagcg cggatctaga cgaacgtggt gttgaaccgt 33000

ttaagtttcc tgaacgtccg ttaatgcgtg ttgagtcaga cttgtctgca ccgaaaagca 33060

aaggtgtgac accgattaag cattttgaag cgcctgctgt tgctggtcat catagagtgc 33120

ctaaccaagc accgtttaca ccttggcata tgtttgagtt tgcgacgggt aatatttcta 33180

actgtttcgg tcctgatttt gatgtttatg aaggtcgtat tccacctcgt acaccttgtg 33240

gcgatttaca agttgttact caggttgtag aagtgcaggg cgaacgtctt gatcttaaaa 33300

atccatcaag ctgtgtagct gaatactatg taccggaaga cgcttggtac tttactaaaa 33360

acagccatga aaactggatg ccttattcat taatcatgga aattgcattg caaccaaatg 33420

gctttatttc tggttacatg ggcacgacgc ttaaataccc tgaaaaagat ctgttcttcc 33480

gtaaccttga tggtagcggc acgttattaa agcagattga tttacgcggc aagaccattg 33540

tgaataaatc agtcttggtt agtacggcta ttgctggtgg cgcgattatt caaagtttca 33600

cgtttgatat gtctgtagat ggcgagctat tttatactgg taaagctgta tttggttact 33660

ttagtggtga atcactgact aaccaactgg gcattgataa cggtaaaacg actaatgcgt 33720

ggtttgttga taacaatacc cccgcagcga atattgatgt gtttgattta actaatcagt 33780

cattggctct gtataaagcg cctgtggata aaccgcatta taaattggct ggtggtcaga 33840

tgaactttat cgatacagtg tcagtggttg aaggcggtgg taaagcgggc gtggcttatg 33900

tttatggcga acgtacgatt gatgctgatg attggttctt ccgttatcac ttccaccaag 33960

atccggtgat gccaggttca ttaggtgttg aagctattat tgagttgatg cagacctatg 34020

cgcttaaaaa tgatttgggt ggcaagtttg ctaacccacg tttcattgcg ccgatgacgc 34080

aagttgattg gaaataccgt gggcaaatta cgccgctgaa taaacagatg tcactggacg 34140

tgcatatcac tgagatcgtg aatgacgctg gtgaagtgcg aatcgttggt gatgcgaatc 34200

tgtctaaaga tggtctgcgt atttatgaag ttaaaaacat cgttttaagt attgttgaag 34260

cgtaaagggt caagtgtaac gtgcttaagc gccgcattgg ttaaagacgc tttgcacgcc 34320

gtgaatccgt ccatggaggc ttggggttgg catccatgcc aacaacagca agcttacttt 34380

aatcaatacg gcttggtgtc catttagacg cctcgaactt agtagttaat agacaaaata 34440

atttagctgt ggaatgaata tagtaagtaa tcattcggca gctacaaaaa aggaattaag 34500

aatgtcgagt ttaggtttta acaataacaa cgcaattaac tgggcttgga aagtagatcc 34560

agcgtcagtt catacacaag atgcagaaat taaagcagct ttaatggatc taactaaacc 34620

tctctatgtg gcgaataatt caggcgtaac tggtatagct aatcatacgt cagtagcagg 34680

tgcgatcagc aataacatcg atgttgatgt attggcgttt gcgcaaaagt taaacccaga 34740

agatctgggt gatgatgctt acaagaaaca gcacggcgtt aaatatgctt atcatggcgg 34800

tgcgatggca aatggtattg cctcggttga attggttgtt gcgttaggta aagcagggct 34860

gttatgttca tttggtgctg caggtctagt gcctgatgcg gttgaagatg caattcgtcg 34920

tattcaagct gaattaccaa atggccctta tgcggttaac ttgatccatg caccagcaga 34980

agaagcatta gagcgtggcg cggttgaacg tttcctaaaa cttggcgtca agacggtaga 35040

ggcttcagct taccttggtt taactgaaca cattgtttgg tatcgtgctg ctggtctaac 35100

taaaaacgca gatggcagtg ttaatatcgg taacaaggtt atcgctaaag tatcgcgtac 35160

cgaagttggt cgccgcttta tggaacctgc accgcaaaaa ttactggata agttattaga 35220

acaaaataag atcacccctg aacaagctgc tttagcgttg cttgtaccta tggctgatga 35280

tattactggg gaagcggatt ctggtggtca tacagataac cgtccgtttt taacattatt 35340

accgacgatt attggtctgc gtgatgaagt gcaagcgaag tataacttct ctcctgcatt 35400

acgtgttggt gctggtggtg gtatcggaac gcctgaagca gcactcgctg catttaacat 35460

gggcgcggct tatatcgttc tgggttctgt gaatcaggcg tgtgttgaag cgggtgcatc 35520

tgaatatact cgtaaactgt tatcgacagt tgaaatggct gatgtgacta tggcacctgc 35580

tgcagatatg tttgaaatgg gtgtgaagct gcaagtatta aaacgcggtt ctatgttcgc 35640

gatgcgtgcg aagaaactgt atgacttgta tgtggcttat gactcgattg aagatatccc 35700

agctgctgaa cgtgagaaga ttgaaaaaca aatcttccgt gcaaacctag acgagatttg 35760

ggatggcact atcgctttct ttactgaacg cgatccagaa atgctagccc gtgcaacgag 35820

tagtcctaaa cgtaaaatgg cacttatctt ccgttggtat cttggccttt cttcacgctg 35880

gtcaaacaca ggcgagaagg gacgtgaaat ggattatcag atttgggcag gcccaagttt 35940

aggtgcattc aacagctggg tgaaaggttc ttaccttgaa gactataccc gccgtggcgc 36000

tgtagatgtt gctttgcata tgcttaaagg tgctgcgtat ttacaacgtg taaaccagtt 36060

gaaattgcaa ggtgttagct taagtacaga attggcaagt tatcgtacga gtgattaatg 36120

ttacttgatg atatgtgaat taattaaagc gcctgagggc gctttttttg gtttttaact 36180

caggtgttgt aactcgaaat tgcccctttc aagttagatc gattactcac tcacaatatg 36240

ttgatatcgc acttgccata tacttgctca tccaaagccc tatattgata atggtgttaa 36300

tagtctttaa tatccgagtc tttcttcagc ataatactaa tatagagact cgaccaatgt 36360

taaacacaac aaagaatata ttcttgtgta ctgccttatt attaacgagt gcgagtacga 36420

cagctactac gctaaacaat tcgatatcag caattgaaca acgtatttct ggtcgtatcg 36480

gtgtggctgt tttagatacg caaaataaac aaacgtgggc ttacaatggt gatgcacatt 36540

ttccgatgat gagtacattc aaaaccctcg cttgcgcgaa aatgctaagt gaatcgacaa 36600

atggtaatct ggatcccagt actagctcat tgataaaggc tgaagaatta atcccttggt 36660

caccagtcac taaaacgttt gtgaataaca ctattacagt ggcgaaagcg tgtgaagcaa 36720

caatgctgac cagtgataat accgcggcta atattgtttt acagtatatc ggaggccctc 36780

aaggcgttac tgcattcttg cgagaaattg gtgatgaaga gagtcagtta gatcgtatag 36840

aacctgaatt gaatgaagct aaggtcggag acttgcgtga taccacgaca ccgaaagcca 36900

tagttaccac gctcaacaaa ctactacttg gtgatgttct acttgatttg gataaaaacc 36960

aacttaaaac atggatgcaa aataataaag tgtcagatcc tttactgcgt tctatattac 37020

cgcaaggctg gtttattgcc gaccgctcag gtgcgggtgg taatggttct cgaggtataa 37080

ctgctatgct ttggcactcc gagcgtcaac cgctaatcat cagtatttat ttaaccgaaa 37140

ctgagttagc aatggcaatg cgcaatgaga ttattgttga gatcggtaag ctgatattca 37200

aagaatacgc ggtgaaataa taagttattt tttgataata ctttaacgag cgtagctatc 37260

gaagtgaggg cgtcaattag acacctttgc ttcccctaca aaatctaatg tgtattacct 37320

cggctagtac aattgcccta agttatttct gtccagcttt ggcttagtgc aattgcgtta 37380

gccaatgtga acaccaaggg actttgtcgt accataacta ccaagcgact ttgtcgtttt 37440

tatcttttct tagacaaaca gaggttaaat gagtgacgcc ttccaaatca caggaatgaa 37500

tccgcatttc aataaaatct aacccgtacc aactccgtac aagttgatct ttagttgttt 37560

aaaatctata ataaattcaa ttacggaatt aatccgtaca actggaggtt ttatggctac 37620

tgcaagactt gatatccgtt tggatgaaga aatcaaagct aaggctgaga aagcatcagc 37680

tttactcggc ttaaaaagtt taaccgaata cgttgttcgc ttaatggacg aagattcaac 37740

taaagtagtt tctgagcatg agagtattac cgttgaagcg aatgtattcg accaatttat 37800

ggctgcttgt gatgaagcga aagccccaaa taaagcatta cttgaagccg ctgtatttac 37860

tcagaatggt gagtttaagt gagttattcc aaacgtttca aagaactgga taaatcaaaa 37920

catgacagag catcatttga ctgtggcgaa aaagagctaa atgattttat ccaaactcaa 37980

gcagccaaac atatgcaagc aggtattagc cgcactctgg ttttacctgc ttctgcgccg 38040

ttaccaaaca aaaaatatcc aatttgctca ttttatagta tcgcgccaag ctcaattagc 38100

cgcgatacgt taccacaagc aatggctaaa aagttaccac gttatcctat ccctgttttt 38160

cttttggctc aacttgccgt ccataaagag tttcatggga gtgggttagg caaagttagc 38220

ttaattaaag cgttagagta cctttgggaa attaactctc acatgagagc ttacgccatc 38280

gttgttgatt gtttaactga acaagctgag tcattctacg ctaaatatgg tttcgacgtt 38340

ctctgcgaaa taaatggtcg agtaagaatg ttcatatcaa tgaaaacagt caatcagtta 38400

ttcacttaac agtaagagtt agtataacag ttgtatgaat taaatttatt atattcggta 38460

atctcattgc gatcacgcta gaagtgcgag cgggtcagac cgaggccaca atagcagccg 38520

ttacgtttag gggatgactt aaaaagataa ctactacgtc agtggcgatc ctagaggatt 38580

aaaggtttat gattcacaac atttatttat tgtgcttaat tttttctatc caatatgcgc 38640

aagctgtaaa tatcactgaa gtagactttt atgtcagtga tgatatccct aaagatgttg 38700

ccaaattaaa gataggtgaa tccataacga actccagcct tattctaagt aactcatcta 38760

ttccactctc gcgggagacg ggtaacatat attactcttc atcaattgct aacttgaact 38820

atgactcgat agaatttgtt atggctcaat tgatggccga agattccagc ctttacaaga 38880

tgctggtaaa tagcgatagg ttgtccgtgc tagtaatgac atcttcccag tccacagatc 38940

tctatggctc gacttactcg gcttattttc ctaatgttgc ggtcatcgat ttgaattgtg 39000

actcgctaac tttagaacat gagctcggcc atctatacgg agctgaacat gaagaaatat 39060

atgacgacta tgtcttctat gctgcgatat gtggagacta tacgactatc atgaactcta 39120

tgcagcctga aatgaaagaa aaacaaatga taaaggcata ttcattccct gaattaaaag 39180

tggatggctt gcagtgcgga aatgaaaata cgaataacaa aaaggttatt ttagacaata 39240

ttggtcggtt tagataggat tgggatatta ttctcattcg gctctactta gtgctgttat 39300

tatgagtgcc agtgcttcta tctacgatat tggtcttaac aagtatttat ctatagacgc 39360

taaggtgtta tgtatttaag ggatgttcaa gatgaaacta ggtgtaaacg atgtatagtt 39420

gtataacatt ttttcaacgg ttggaacgtt cgattctatc gggtaacaag accgcgacga 39480

tccgcgataa gtccgatagt cattacttag ttggtcagat gttagatgct tgtactcacg 39540

aagataatcg gaaaatgtgt caaatagaaa tactgagcat tgaatatgtg acgtttagtg 39600

aattaaaccg tgcgcacgcc aatgctgaag gtttaccgtt tttgtttatg cttaagtgga 39660

tagttcgaaa gatttatccg acttcaaatg atttattttt cataagtttc agagttgtaa 39720

ctatcgatat cttataagtc ttagtgcaca aaacagaact atttatagcg ctcaagaagg 39780

cgataatttg ataatgaatt atcgccttgt tactattaag agactttaaa tgactgagat 39840

ataagatatg acacggaaga acatattgat cacaggcgca agttcagggt tgggccgagg 39900

tatggccatc gaatttgcaa aatcaggtca taacttagca ctttgtgcac gtagacttga 39960

taatttagtt gcactgaaag cagaactctt agccctcaat cctcacatcc aaatcgaaat 40020

aaaacctctt gatgtcaatg aacatgaaca agtcttcact gttttccatg aattcaaagc 40080

tgaatttggt acgcttgatc gtattattgt taatgctgga ttaggcaagg gtggatcc 40138

13

19227

Vibrio marinus

13

aaatgcaatt aattatggcg taaatagagt gaaaacatgg ctaatattca ctaagtcctg 60

aattttatat aaagtttaat ctgttatttt agcgtttacc tggtcttatc agtgaggttt 120

atagccatta ttagtgggat tgaagtgatt tttaaagcta tgtatattat tgcaaatata 180

aattgtaaca attaagactt tggacacttg agttcaattt cgaattgatt ggcataaaat 240

ttaaaacagc taaatctacc tcaatcattt tagcaaatgt atgcaggtag atttttttcg 300

ccatttaaga gtacacttgt acgctaggtt tttgtttagt gtgcaaatga acgttttgat 360

gagcattgtt tttagagcac aaaatagatc cttacaggag caataacgca atggctaaaa 420

agaacaccac atcgattaag cacgccaagg atgtgttaag tagtgatgat caacagttaa 480

attctcgctt gcaagaatgt ccgattgcca tcattggtat ggcatcggtt tttgcagatg 540

ctaaaaactt ggatcaattc tgggataaca tcgttgactc tgtggacgct attattgatg 600

tgcctagcga tcgctggaac attgacgacc attactcggc tgataaaaaa gcagctgaca 660

agacatactg caaacgcggt ggtttcattc cagagcttga ttttgatccg atggagtttg 720

gtttaccgcc aaatatcctc gagttaactg acatcgctca attgttgtca ttaattgttg 780

ctcgtgatgt attaagtgat gctggcattg gtagtgatta tgaccatgat aaaattggta 840

tcacgctggg tgtcggtggt ggtcagaaac aaatttcgcc attaacgtcg cgcctacaag 900

gcccggtatt agaaaaagta ttaaaagcct caggcattga tgaagatgat cgcgctatga 960

tcatcgacaa atttaaaaaa gcctacatcg gctgggaaga gaactcattc ccaggcatgc 1020

taggtaacgt tattgctggt cgtatcgcca atcgttttga ttttggtggt actaactgtg 1080

tggttgatgc ggcatgcgct ggctcccttg cagctgttaa aatggcgatc tcagacttac 1140

ttgaatatcg ttcagaagtc atgatatcgg gtggtgtatg ttgtgataac tcgccattca 1200

tgtatatgtc attctcgaaa acaccagcat ttaccaccaa tgatgatatc cgtccgtttg 1260

atgacgattc aaaaggcatg ctggttggtg aaggtattgg catgatggcg tttaaacgtc 1320

ttgaagatgc tgaacgtgac ggcgacaaaa tttattctgt actgaaaggt atcggtacat 1380

cttcagatgg tcgtttcaaa tctatttacg ctccacgccc agatggccaa gcaaaagcgc 1440

taaaacgtgc ttatgaagat gccggttttg cccctgaaac atgtggtcta attgaaggcc 1500

atggtacggg taccaaagcg ggtgatgccg cagaatttgc tggcttgacc aaacactttg 1560

gcgccgccag tgatgaaaag caatatatcg ccttaggctc agttaaatcg caaattggtc 1620

atactaaatc tgcggctggc tctgcgggta tgattaaggc ggcattagcg ctgcatcata 1680

aaatcttacc tgcaacgatc catatcgata aaccaagtga agccttggat atcaaaaaca 1740

gcccgttata cctaaacagc gaaacgcgtc cttggatgcc acgtgaagat ggtattccac 1800

gtcgtgcagg tatcagctca tttggttttg gcggcaccaa cttccatatt attttagaag 1860

agtatcgccc aggtcacgat agcgcatatc gcttaaactc agtgagccaa actgtgttga 1920

tctcggcaaa cgaccaacaa ggtattgttg ctgagttaaa taactggcgt actaaactgg 1980

ctgtcgatgc tgatcatcaa gggtttgtat ttaatgagtt agtgacaacg tggccattaa 2040

aaaccccatc cgttaaccaa gctcgtttag gttttgttgc gcgtaatgca aatgaagcga 2100

tcgcgatgat tgatacggca ttgaaacaat tcaatgcgaa cgcagataaa atgacatggt 2160

cagtacctac cggggtttac tatcgtcaag ccggtattga tgcaacaggt aaagtggttg 2220

cgctattctc agggcaaggt tcgcaatacg tgaacatggg tcgtgaatta acctgtaact 2280

tcccaagcat gatgcacagt gctgcggcga tggataaaga gttcagtgcc gctggtttag 2340

gccagttatc tgcagttact ttccctatcc ctgtttatac ggatgccgag cgtaagctac 2400

aagaagagca attacgttta acgcaacatg cgcaaccagc gattggtagt ttgagtgttg 2460

gtctgttcaa aacgtttaag caagcaggtt ttaaagctga ttttgctgcc ggtcatagtt 2520

tcggtgagtt aaccgcatta tgggctgccg atgtattgag cgaaagcgat tacatgatgt 2580

tagcgcgtag tcgtggtcaa gcaatggctg cgccagagca acaagatttt gatgcaggta 2640

agatggccgc tgttgttggt gatccaaagc aagtcgctgt gatcattgat acccttgatg 2700

atgtctctat tgctaacttc aactcgaata accaagttgt tattgctggt actacggagc 2760

aggttgctgt agcggttaca accttaggta atgctggttt caaagttgtg ccactgccgg 2820

tatctgctgc gttccataca cctttagttc gtcacgcgca aaaaccattt gctaaagcgg 2880

ttgatagcgc taaatttaaa gcgccaagca ttccagtgtt tgctaatggc acaggcttgg 2940

tgcattcaag caaaccgaat gacattaaga aaaacctgaa aaaccacatg ctggaatctg 3000

ttcatttcaa tcaagaaatt gacaacatct atgctgatgg tggccgcgta tttatcgaat 3060

ttggtccaaa gaatgtatta actaaattgg ttgaaaacat tctcactgaa aaatctgatg 3120

tgactgctat cgcggttaat gctaatccta aacaacctgc ggacgtacaa atgcgccaag 3180

ctgcgctgca aatggcagtg cttggtgtcg cattagacaa tattgacccg tacgacgccg 3240

ttaagcgtcc acttgttgcg ccgaaagcat caccaatgtt gatgaagtta tctgcagcgt 3300

cttatgttag tccgaaaacg aagaaagcgt ttgctgatgc attgactgat ggctggactg 3360

ttaagcaagc gaaagctgta cctgctgttg tgtcacaacc acaagtgatt gaaaagatcg 3420

ttgaagttga aaagatagtt gaacgcattg tcgaagtaga gcgtattgtc gaagtagaaa 3480

aaatcgtcta cgttaatgct gacggttcgc ttatatcgca aaataatcaa gacgttaaca 3540

gcgctgttgt tagcaacgtg actaatagct cagtgactca tagcagtgat gctgaccttg 3600

ttgcctctat tgaacgcagt gttggtcaat ttgttgcaca ccaacagcaa ttattaaatg 3660

tacatgaaca gtttatgcaa ggtccacaag actacgcgaa aacagtgcag aacgtacttg 3720

ctgcgcagac gagcaatgaa ttaccggaaa gtttagaccg tacattgtct atgtataacg 3780

agttccaatc agaaacgcta cgtgtacatg aaacgtacct gaacaatcag acgagcaaca 3840

tgaacaccat gcttactggt gctgaagctg atgtgctagc aaccccaata actcaggtag 3900

tgaatacagc cgttgccact agtcacaagg tagttgctcc agttattgct aatacagtga 3960

cgaatgttgt atctagtgtc agtaataacg cggcggttgc agtgcaaact gtggcattag 4020

cgcctacgca agaaatcgct ccaacagtcg ctactacgcc agcacccgca ttggttgcta 4080

tcgtggctga acctgtgatt gttgcgcatg ttgctacaga agttgcacca attacaccat 4140

cagttacacc agttgtcgca actcaagcgg ctatcgatgt agcaactatt aacaaagtaa 4200

tgttagaagt tgttgctgat aaaaccggtt atccaacgga tatgctggaa ctgagcatgg 4260

acatggaagc tgacttaggt atcgactcaa tcaaacgtgt tgagatatta ggcgcagtac 4320

aggaattgat ccctgactta cctgaactta atcctgaaga tcttgctgag ctacgcacgc 4380

ttggtgagat tgtcgattac atgaattcaa aagcccaggc tgtagctcct acaacagtac 4440

ctgtaacaag tgcacctgtt tcgcctgcat ctgctggtat tgatttagcc cacatccaaa 4500

acgtaatgtt agaagtggtt gcagacaaaa ccggttaccc aacagacatg ctagaactga 4560

gcatggatat ggaagctgac ttaggtattg attcaatcaa gcgtgtggaa atcttaggtg 4620

cagtacagga gatcataact gatttacctg agctaaaccc tgaagatctt gctgaattac 4680

gcaccctagg tgaaatcgtt agttacatgc aaagcaaagc gccagtcgct gaaagtgcgc 4740

cagtggcgac ggctcctgta gcaacaagct cagcaccgtc tatcgatttg aaccacattc 4800

aaacagtgat gatggatgta gttgcagata agactggtta tccaactgac atgctagaac 4860

ttggcatgga catggaagct gatttaggta tcgattcaat caaacgtgtg gaaatattag 4920

gcgcagtgca ggagatcatc actgatttac ctgagctaaa cccagaagac ctcgctgaat 4980

tacgcacgct aggtgaaatc gttagttaca tgcaaagcaa agcgccagtc gctgagagtg 5040

cgccagtagc gacggcttct gtagcaacaa gctctgcacc gtctatcgat ttaaaccata 5100

tccaaacagt gatgatggaa gtggttgcag acaaaaccgg ttatccagta gacatgttag 5160

aacttgctat ggacatggaa gctgacctag gtatcgattc aatcaagcgt gtagaaattt 5220

taggtgcggt acaggaaatc attactgact tacctgagct taaccctgaa gatcttgctg 5280

aactacgtac attaggtgaa atcgttagtt acatgcaaag caaagcgccc gtagctgaag 5340

cgcctgcagt acctgttgca gtagaaagtg cacctactag tgtaacaagc tcagcaccgt 5400

ctatcgattt agaccacatc caaaatgtaa tgatggatgt tgttgctgat aagactggtt 5460

atcctgccaa tatgcttgaa ttagcaatgg acatggaagc cgaccttggt attgattcaa 5520

tcaagcgtgt tgaaattcta ggcgcggtac aggagatcat tactgattta cctgaactaa 5580

acccagaaga cttagctgaa ctacgtacgt tagaagaaat tgtaacctac atgcaaagca 5640

aggcgagtgg tgttactgta aatgtagtgg ctagccctga aaataatgct gtatcagatg 5700

catttatgca aagcaatgtg gcgactatca cagcggccgc agaacataag gcggaattta 5760

aaccggcgcc gagcgcaacc gttgctatct ctcgtctaag ctctatcagt aaaataagcc 5820

aagattgtaa aggtgctaac gccttaatcg tagctgatgg cactgataat gctgtgttac 5880

ttgcagacca cctattgcaa actggctgga atgtaactgc attgcaacca acttgggtag 5940

ctgtaacaac gacgaaagca tttaataagt cagtgaacct ggtgacttta aatggcgttg 6000

atgaaactga aatcaacaac attattactg ctaacgcaca attggatgca gttatctatc 6060

tgcacgcaag tagcgaaatt aatgctatcg aatacccaca agcatctaag caaggcctga 6120

tgttagcctt cttattagcg aaattgagta aagtaactca agccgctaaa gtgcgtggcg 6180

cctttatgat tgttactcag cagggtggtt cattaggttt tgatgatatc gattctgcta 6240

caagtcatga tgtgaaaaca gacctagtac aaagcggctt aaacggttta gttaagacac 6300

tgtctcacga gtgggataac gtattctgtc gtgcggttga tattgcttcg tcattaacgg 6360

ctgaacaagt tgcaagcctt gttagtgatg aactacttga tgctaacact gtattaacag 6420

aagtgggtta tcaacaagct ggtaaaggcc ttgaacgtat cacgttaact ggtgtggcta 6480

ctgacagcta tgcattaaca gctggcaata acatcgatgc taactcggta tttttagtga 6540

gtggtggcgc aaaaggtgta actgcacatt gtgttgctcg tatagctaaa gaatatcagt 6600

ctaagttcat cttattggga cgttcaacgt tctcaagtga cgaaccgagc tgggcaagtg 6660

gtattactga tgaagcggcg ttaaagaaag cagcgatgca gtctttgatt acagcaggtg 6720

ataaaccaac acccgttaag atcgtacagc taatcaaacc aatccaagct aatcgtgaaa 6780

ttgcgcaaac cttgtctgca attaccgctg ctggtggcca agctgaatat gtttctgcag 6840

atgtaactaa tgcagcaagc gtacaaatgg cagtcgctcc agctatcgct aagttcggtg 6900

caatcactgg catcattcat ggcgcgggtg tgttagctga ccaattcatt gagcaaaaaa 6960

cactgagtga ttttgagtct gtttacagca ctaaaattga cggtttgtta tcgctactat 7020

cagtcactga agcaagcaac atcaagcaat tggtattgtt ctcgtcagcg gctggtttct 7080

acggtaaccc cggccagtct gattactcga ttgccaatga gatcttaaat aaaaccgcat 7140

accgctttaa atcattgcac ccacaagctc aagtattgag ctttaactgg ggtccttggg 7200

acggtggcat ggtaacgcct gagcttaaac gtatgtttga ccaacgtggt gtttacatta 7260

ttccacttga tgcaggtgca cagttattgc tgaatgaact agccgctaat gataaccgtt 7320

gtccacaaat cctcgtgggt aatgacttat ctaaagatgc tagctctgat caaaagtctg 7380

atgaaaagag tactgctgta aaaaagccac aagttagtcg tttatcagat gctttagtaa 7440

ctaaaagtat caaagcgact aacagtagct ctttatcaaa caagactagt gctttatcag 7500

acagtagtgc ttttcaggtt aacgaaaacc actttttagc tgaccacatg atcaaaggca 7560

atcaggtatt accaacggta tgcgcgattg cttggatgag tgatgcagca aaagcgactt 7620

atagtaaccg agactgtgca ttgaagtatg tcggtttcga agactataaa ttgtttaaag 7680

gtgtggtttt tgatggcaat gaggcggcgg attaccaaat ccaattgtcg cctgtgacaa 7740

gggcgtcaga acaggattct gaagtccgta ttgccgcaaa gatctttagc ctgaaaagtg 7800

acggtaaacc tgtgtttcat tatgcagcga caatattgtt agcaactcag ccacttaatg 7860

ctgtgaaggt agaacttccg acattgacag aaagtgttga tagcaacaat aaagtaactg 7920

atgaagcaca agcgttatac agcaatggca ccttgttcca cggtgaaagt ctgcagggca 7980

ttaagcagat attaagttgt gacgacaagg gcctgctatt ggcttgtcag ataaccgatg 8040

ttgcaacagc taagcaggga tccttcccgt tagctgacaa caatatcttt gccaatgatt 8100

tggtttatca ggctatgttg gtctgggtgc gcaaacaatt tggtttaggt agcttacctt 8160

cggtgacaac ggcttggact gtgtatcgtg aagtggttgt agatgaagta ttttatctgc 8220

aacttaatgt tgttgagcat gatctattgg gttcacgcgg cagtaaagcc cgttgtgata 8280

ttcaattgat tgctgctgat atgcaattac ttgccgaagt gaaatcagcg caagtcagtg 8340

tcagtgacat tttgaacgat atgtcatgat cgagtaaata ataacgatag gcgtcatggt 8400

gagcatggcg tctgctttct tcatttttta acattaacaa tattaatagc taaacgcggt 8460

tgctttaaac caagtaaaca agtgctttta gctattacta ttccaaacag gatattaaag 8520

agaatatgac ggaattagct gttattggta tggatgctaa atttagcgga caagacaata 8580

ttgaccgtgt ggaacgcgct ttctatgaag gtgcttatgt aggtaatgtt agccgcgtta 8640

gtaccgaatc taatgttatt agcaatggcg aagaacaagt tattactgcc atgacagttc 8700

ttaactctgt cagtctacta gcgcaaacga atcagttaaa tatagctgat atcgcggtgt 8760

tgctgattgc tgatgtaaaa agtgctgatg atcagcttgt agtccaaatt gcatcagcaa 8820

ttgaaaaaca gtgtgcgagt tgtgttgtta ttgctgattt aggccaagca ttaaatcaag 8880

tagctgattt agttaataac caagactgtc ctgtggctgt aattggcatg aataactcgg 8940

ttaatttatc tcgtcatgat cttgaatctg taactgcaac aatcagcttt gatgaaacct 9000

tcaatggtta taacaatgta gctgggttcg cgagtttact tatcgcttca actgcgtttg 9060

ccaatgctaa gcaatgttat atatacgcca acattaaggg cttcgctcaa tcgggcgtaa 9120

atgctcaatt taacgttgga aacattagcg atactgcaaa gaccgcattg cagcaagcta 9180

gcataactgc agagcaggtt ggtttgttag aagtgtcagc agtcgctgat tcggcaatcg 9240

cattgtctga aagccaaggt ttaatgtctg cttatcatca tacgcaaact ttgcatactg 9300

cattaagcag tgcccgtagt gtgactggtg aaggcgggtg tttttcacag gtcgcaggtt 9360

tattgaaatg tgtaattggt ttacatcaac gttatattcc ggcgattaaa gattggcaac 9420

aaccgagtga caatcaaatg tcacggtggc ggaattcacc attctatatg cctgtagatg 9480

ctcgaccttg gttcccacat gctgatggct ctgcacacat tgccgcttat agttgtgtga 9540

ctgctgacag ctattgtcat attcttttac aagaaaacgt cttacaagaa cttgttttga 9600

aagaaacagt cttgcaagat aatgacttaa ctgaaagcaa gcttcagact cttgaacaaa 9660

acaatccagt agctgatctg cgcactaatg gttactttgc atcgagcgag ttagcattaa 9720

tcatagtaca aggtaatgac gaagcacaat tacgctgtga attagaaact attacagggc 9780

agttaagtac tactggcata agtactatca gtattaaaca gatcgcagca gactgttatg 9840

cccgtaatga tactaacaaa gcctatagcg cagtgcttat tgccgagact gctgaagagt 9900

taagcaaaga aataaccttg gcgtttgctg gtatcgctag cgtgtttaat gaagatgcta 9960

aagaatggaa aaccccgaag ggcagttatt ttaccgcgca gcctgcaaat aaacaggctg 10020

ctaacagcac acagaatggt gtcaccttca tgtacccagg tattggtgct acatatgttg 10080

gtttagggcg tgatctattt catctattcc cacagattta tcagcctgta gcggctttag 10140

ccgatgacat tggcgaaagt ctaaaagata ctttacttaa tccacgcagt attagtcgtc 10200

atagctttaa agaactcaag cagttggatc tggacctgcg cggtaactta gccaatatcg 10260

ctgaagccgg tgtgggtttt gcttgtgtgt ttaccaaggt atttgaagaa gtctttgccg 10320

ttaaagctga ctttgctaca ggttatagca tgggtgaagt aagcatgtat gcagcactag 10380

gctgctggca gcaaccggga ttgatgagtg ctcgccttgc acaatcgaat acctttaatc 10440

atcaactttg cggcgagtta agaacactac gtcagcattg gggcatggat gatgtagcta 10500

acggtacgtt cgagcagatc tgggaaacct ataccattaa ggcaacgatt gaacaggtcg 10560

aaattgcctc tgcagatgaa gatcgtgtgt attgcaccat tatcaataca cctgatagct 10620

tgttgttagc cggttatcca gaagcctgtc agcgagtcat taagaattta ggtgtgcgtg 10680

caatggcatt gaatatggcg aacgcaattc acagcgcgcc agcttatgcc gaatacgatc 10740

atatggttga gctataccat atggatgtta ctccacgtat taataccaag atgtattcaa 10800

gctcatgtta tttaccgatt ccacaacgca gcaaagcgat ttcccacagt attgctaaat 10860

gtttgtgtga tgtggtggat ttcccacgtt tggttaatac cttacatgac aaaggtgcgc 10920

gggtattcat tgaaatgggt ccaggtcgtt cgttatgtag ctgggtagat aagatcttag 10980

ttaatggcga tggcgataat aaaaagcaaa gccaacatgt atctgttcct gtgaatgcca 11040

aaggcaccag tgatgaactt acttatattc gtgcgattgc taagttaatt agtcatggcg 11100

tgaatttgaa tttagatagc ttgtttaacg ggtcaatcct ggttaaagca ggccatatag 11160

caaacacgaa caaatagtca acatcgatat ctagcgctgg tgagttatac ctcattagtt 11220

gaaatatgga tttaaagaga gtaattatgg aaaatattgc agtagtaggt attgctaatt 11280

tgttcccggg ctcacaagca ccggatcaat tttggcagca attgcttgaa caacaagatt 11340

gccgcagtaa ggcgaccgct gttcaaatgg gcgttgatcc tgctaaatat accgccaaca 11400

aaggtgacac agataaattt tactgtgtgc acggcggtta catcagtgat ttcaattttg 11460

atgcttcagg ttatcaactc gataatgatt atttagccgg tttagatgac cttaatcaat 11520

gggggcttta tgttacgaaa caagccctta ccgatgcggg ttattggggc agtactgcac 11580

tagaaaactg tggtgtgatt ttaggtaatt tgtcattccc aactaaatca tctaatcagc 11640

tgtttatgcc tttgtatcat caagttgttg ataatgcctt aaaggcggta ttacatcctg 11700

attttcaatt aacgcattac acagcaccga aaaaaacaca tgctgacaat gcattagtag 11760

caggttatcc agctgcattg atcgcgcaag cggcgggtct tggtggttca cattttgcac 11820

tggatgcggc ttgtgcttca tcttgttata gcgttaagtt agcgtgtgat tacctgcata 11880

cgggtaaagc caacatgatg cttgctggtg cggtatctgc agcagatcct atgttcgtaa 11940

atatgggttt ctcgatattc caagcttacc cagctaacaa tgtacatgcc ccgtttgacc 12000

aaaattcaca aggtctattt gccggtgaag gcgcgggcat gatggtattg aaacgtcaaa 12060

gtgatgcagt acgtgatggt gatcatattt acgccattat taaaggcggc gcattatcga 12120

atgacggtaa aggcgagttt gtattaagcc cgaacaccaa gggccaagta ttagtatatg 12180

aacgtgctta tgccgatgca gatgttgacc cgagtacagt tgactatatt gaatgtcatg 12240

caacgggcac acctaagggt gacaatgttg aattgcgttc gatggaaacc tttttcagtc 12300

gcgtaaataa caaaccatta ctgggctcgg ttaaatctaa ccttggtcat ttgttaactg 12360

ccgctggtat gcctggcatg accaaagcta tgttagcgct aggtaaaggt cttattcctg 12420

caacgattaa cttaaagcaa ccactgcaat ctaaaaacgg ttactttact ggcgagcaaa 12480

tgccaacgac gactgtgtct tggccaacaa ctccgggtgc caaggcagat aaaccgcgta 12540

ccgcaggtgt gagcgtattt ggttttggtg gcagcaacgc ccatttggta ttacaacagc 12600

caacgcaaac actcgagact aattttagtg ttgctaaacc acgtgagcct ttggctatta 12660

ttggtatgga cagccatttt ggtagtgcca gtaatttagc gcagttcaaa accttattaa 12720

ataataatca aaataccttc cgtgaattac cagaacaacg ctggaaaggc atggaaagta 12780

acgctaacgt catgcagtcg ttacaattac gcaaagcgcc taaaggcagt tacgttgaac 12840

agctagatat tgatttcttg cgttttaaag taccgcctaa tgaaaaagat tgcttgatcc 12900

cgcaacagtt aatgatgatg caagtggcag acaatgctgc gaaagacgga ggtctagttg 12960

aaggtcgtaa tgttgcggta ttagtagcga tgggcatgga actggaatta catcagtatc 13020

gtggtcgcgt taatctaacc acccaaattg aagacagctt attacagcaa ggtattaacc 13080

tgactgttga gcaacgtgaa gaactgacca atattgctaa agacggtgtt gcctcggctg 13140

cacagctaaa tcagtatacg agtttcattg gtaatattat ggcgtcacgt atttcggcgt 13200

tatgggattt ttctggtcct gctattaccg tatcggctga agaaaactct gtttatcgtt 13260

gtgttgaatt agctgaaaat ctatttcaaa ccagtgatgt tgaagccgtt attattgctg 13320

ctgttgattt gtctggttca attgaaaaca ttactttacg tcagcactac ggtccagtta 13380

atgaaaaggg atctgtaagt gaatgtggtc cggttaatga aagcagttca gtaaccaaca 13440

atattcttga tcagcaacaa tggctggtgg gtgaaggcgc agcggctatt gtcgttaaac 13500

cgtcatcgca agtcactgct gagcaagttt atgcgcgtat tgatgcggtg agttttgccc 13560

ctggtagcaa tgcgaaagca attacgattg cagcggataa agcattaaca cttgctggta 13620

tcagtgctgc tgatgtagct agtgttgaag cacatgcaag tggttttagt gccgaaaata 13680

atgctgaaaa aaccgcgtta ccgactttat acccaagcgc aagtatcagt tcggtgaaag 13740

ccaatattgg tcatacgttt aatgcctcgg gtatggcgag tattattaaa acggcgctgc 13800

tgttagatca gaatacgagt caagatcaga aaagcaaaca tattgctatt aacggtctag 13860

gtcgtgataa cagctgcgcg catcttatct tatcgagttc agcgcaagcg catcaagttg 13920

caccagcgcc tgtatctggt atggccaagc aacgcccaca gttagttaaa accatcaaac 13980

tcggtggtca gttaattagc aacgcgattg ttaacagtgc gagttcatct ttacacgcta 14040

ttaaagcgca gtttgccggt aagcacttaa acaaagttaa ccagccagtg atgatggata 14100

acctgaagcc ccaaggtatt agcgctcatg caaccaatga gtatgtggtg actggagctg 14160

ctaacactca agcttctaac attcaagcat ctcatgttca agcgtcaagt catgcacaag 14220

agatagcacc aaaccaagtt caaaatatgc aagctacagc agccgctgta agttcacccc 14280

tttctcaaca tcaacacaca gcgcagcccg tagcggcacc gagcgttgtt ggagtgactg 14340

tgaaacataa agcaagtaac caaattcatc agcaagcgtc tacgcataaa gcatttttag 14400

aaagtcgttt agctgcacag aaaaacctat cgcaacttgt tgaattgcaa accaagctgt 14460

caatccaaac tggtagtgac aatacatcta acaatactgc gtcaacaagc aatacagtgc 14520

taacaaatcc tgtatcagca acgccattaa cacttgtgtc taatgcgcct gtagtagcga 14580

caaacctaac cagtacagaa gcaaaagcgc aagcagctgc tacacaagct ggttttcaga 14640

taaaaggacc tgttggttac aactatccac cgctgcagtt aattgaacgt tataataaac 14700

cagaaaacgt gatttacgat caagctgatt tggttgaatt cgctgaaggt gatattggta 14760

aggtatttgg tgctgaatac aatattattg atggctattc gcgtcgtgta cgtctgccaa 14820

cctcagatta cttgttagta acacgtgtta ctgaacttga tgccaaggtg catgaataca 14880

agaaatcata catgtgtact gaatatgatg tgcctgttga tgcaccgttc ttaattgatg 14940

gtcagatccc ttggtctgtt gccgtcgaat caggccagtg tgatttgatg ttgatttcat 15000

atatcggtat tgatttccaa gcgaaaggcg aacgtgttta ccgtttactt gattgtgaat 15060

taactttcct tgaagagatg gcttttggtg gcgatacttt acgttacgag atccacattg 15120

attcgtatgc acgtaacggc gagcaattat tattcttctt ccattacgat tgttacgtag 15180

gggataagaa ggtacttatc atgcgtaatg gttgtgctgg tttctttact gacgaagaac 15240

tttctgatgg taaaggcgtt attcataacg acaaagacaa agctgagttt agcaatgctg 15300

ttaaatcatc attcacgccg ttattacaac ataaccgtgg tcaatacgat tataacgaca 15360

tgatgaagtt ggttaatggt gatgttgcca gttgttttgg tccgcaatat gatcaaggtg 15420

gccgtaatcc atcattgaaa ttctcgtctg agaagttctt gatgattgaa cgtattacca 15480

agatagaccc aaccggtggt cattggggac taggcctgtt agaaggtcag aaagatttag 15540

accctgagca ttggtatttc ccttgtcact ttaaaggtga tcaagtaatg gctggttcgt 15600

tgatgtcgga aggttgtggc caaatggcga tgttcttcat gctgtctctt ggtatgcata 15660

ccaatgtgaa caacgctcgt ttccaaccac taccaggtga atcacaaacg gtacgttgtc 15720

gtgggcaagt actgccacag cgcaatacct taacttaccg tatggaagtt actgcgatgg 15780

gtatgcatcc acagccattc atgaaagcta atattgatat tttgcttgac ggtaaagtgg 15840

ttgttgattt caaaaacttg agcgtgatga tcagcgaaca agatgagcat tcagattacc 15900

ctgtaacact gccgagtaat gtggcgctta aagcgattac tgcacctgtt gcgtcagtag 15960

caccagcatc ttcacccgct aacagcgcgg atctagacga acgtggtgtt gaaccgttta 16020

agtttcctga acgtccgtta atgcgtgttg agtcagactt gtctgcaccg aaaagcaaag 16080

gtgtgacacc gattaagcat tttgaagcgc ctgctgttgc tggtcatcat agagtgccta 16140

accaagcacc gtttacacct tggcatatgt ttgagtttgc gacgggtaat atttctaact 16200

gtttcggtcc tgattttgat gtttatgaag gtcgtattcc acctcgtaca ccttgtggcg 16260

atttacaagt tgttactcag gttgtagaag tgcagggcga acgtcttgat cttaaaaatc 16320

catcaagctg tgtagctgaa tactatgtac cggaagacgc ttggtacttt actaaaaaca 16380

gccatgaaaa ctggatgcct tattcattaa tcatggaaat tgcattgcaa ccaaatggct 16440

ttatttctgg ttacatgggc acgacgctta aataccctga aaaagatctg ttcttccgta 16500

accttgatgg tagcggcacg ttattaaagc agattgattt acgcggcaag accattgtga 16560

ataaatcagt cttggttagt acggctattg ctggtggcgc gattattcaa agtttcacgt 16620

ttgatatgtc tgtagatggc gagctatttt atactggtaa agctgtattt ggttacttta 16680

gtggtgaatc actgactaac caactgggca ttgataacgg taaaacgact aatgcgtggt 16740

ttgttgataa caataccccc gcagcgaata ttgatgtgtt tgatttaact aatcagtcat 16800

tggctctgta taaagcgcct gtggataaac cgcattataa attggctggt ggtcagatga 16860

actttatcga tacagtgtca gtggttgaag gcggtggtaa agcgggcgtg gcttatgttt 16920

atggcgaacg tacgattgat gctgatgatt ggttcttccg ttatcacttc caccaagatc 16980

cggtgatgcc aggttcatta ggtgttgaag ctattattga gttgatgcag acctatgcgc 17040

ttaaaaatga tttgggtggc aagtttgcta acccacgttt cattgcgccg atgacgcaag 17100

ttgattggaa ataccgtggg caaattacgc cgctgaataa acagatgtca ctggacgtgc 17160

atatcactga gatcgtgaat gacgctggtg aagtgcgaat cgttggtgat gcgaatctgt 17220

ctaaagatgg tctgcgtatt tatgaagtta aaaacatcgt tttaagtatt gttgaagcgt 17280

aaagggtcaa gtgtaacgtg cttaagcgcc gcattggtta aagacgcttt gcacgccgtg 17340

aatccgtcca tggaggcttg gggttggcat ccatgccaac aacagcaagc ttactttaat 17400

caatacggct tggtgtccat ttagacgcct cgaacttagt agttaataga caaaataatt 17460

tagctgtgga atgaatatag taagtaatca ttcggcagct acaaaaaagg aattaagaat 17520

gtcgagttta ggttttaaca ataacaacgc aattaactgg gcttggaaag tagatccagc 17580

gtcagttcat acacaagatg cagaaattaa agcagcttta atggatctaa ctaaacctct 17640

ctatgtggcg aataattcag gcgtaactgg tatagctaat catacgtcag tagcaggtgc 17700

gatcagcaat aacatcgatg ttgatgtatt ggcgtttgcg caaaagttaa acccagaaga 17760

tctgggtgat gatgcttaca agaaacagca cggcgttaaa tatgcttatc atggcggtgc 17820

gatggcaaat ggtattgcct cggttgaatt ggttgttgcg ttaggtaaag cagggctgtt 17880

atgttcattt ggtgctgcag gtctagtgcc tgatgcggtt gaagatgcaa ttcgtcgtat 17940

tcaagctgaa ttaccaaatg gcccttatgc ggttaacttg atccatgcac cagcagaaga 18000

agcattagag cgtggcgcgg ttgaacgttt cctaaaactt ggcgtcaaga cggtagaggc 18060

ttcagcttac cttggtttaa ctgaacacat tgtttggtat cgtgctgctg gtctaactaa 18120

aaacgcagat ggcagtgtta atatcggtaa caaggttatc gctaaagtat cgcgtaccga 18180

agttggtcgc cgctttatgg aacctgcacc gcaaaaatta ctggataagt tattagaaca 18240

aaataagatc acccctgaac aagctgcttt agcgttgctt gtacctatgg ctgatgatat 18300

tactggggaa gcggattctg gtggtcatac agataaccgt ccgtttttaa cattattacc 18360

gacgattatt ggtctgcgtg atgaagtgca agcgaagtat aacttctctc ctgcattacg 18420

tgttggtgct ggtggtggta tcggaacgcc tgaagcagca ctcgctgcat ttaacatggg 18480

cgcggcttat atcgttctgg gttctgtgaa tcaggcgtgt gttgaagcgg gtgcatctga 18540

atatactcgt aaactgttat cgacagttga aatggctgat gtgactatgg cacctgctgc 18600

agatatgttt gaaatgggtg tgaagctgca agtattaaaa cgcggttcta tgttcgcgat 18660

gcgtgcgaag aaactgtatg acttgtatgt ggcttatgac tcgattgaag atatcccagc 18720

tgctgaacgt gagaagattg aaaaacaaat cttccgtgca aacctagacg agatttggga 18780

tggcactatc gctttcttta ctgaacgcga tccagaaatg ctagcccgtg caacgagtag 18840

tcctaaacgt aaaatggcac ttatcttccg ttggtatctt ggcctttctt cacgctggtc 18900

aaacacaggc gagaagggac gtgaaatgga ttatcagatt tgggcaggcc caagtttagg 18960

tgcattcaac agctgggtga aaggttctta ccttgaagac tatacccgcc gtggcgctgt 19020

agatgttgct ttgcatatgc ttaaaggtgc tgcgtattta caacgtgtaa accagttgaa 19080

attgcaaggt gttagcttaa gtacagaatt ggcaagttat cgtacgagtg attaatgtta 19140

cttgatgata tgtgaattaa ttaaagcgcc tgagggcgct ttttttggtt tttaactcag 19200

gtgttgtaac tcgaaattgc ccctttc 19227

14

217

Shewanella putrefaciens

14

attggtaaaa ataggggtta tgtttgttgc tttaaagagt gtcctgaaaa attgctaact 60

tctcgattga tttccttata cttctgtccg ttaacaatac aagagtgcga taaccagact 120

acagagttgg ttaagtcatg gctgcctgaa gatgagttaa ttaaggttaa tcgctacatt 180

aaacaagaag ctaaaactca aggtttaatg gtaagag 217

15

72

Shewanella putrefaciens

15

Ile Gly Lys Asn Arg Gly Tyr Val Cys Cys Phe Lys Glu Cys Pro Glu

1 5 10 15

Lys Leu Leu Thr Ser Arg Leu Ile Ser Leu Tyr Phe Cys Pro Leu Thr

20 25 30

Ile Gln Glu Cys Asp Asn Gln Thr Thr Glu Leu Val Lys Ser Trp Leu

35 40 45

Pro Glu Asp Glu Leu Ile Lys Val Asn Arg Tyr Ile Lys Gln Glu Ala

50 55 60

Lys Thr Gln Gly Leu Met Val Arg

65 70

16

885

Shewanella putrefaciens

16

agcgaaatgc ttatcaagaa attccaagat caatacatca ctgggaagaa aattcattcc 60

ctggttcact gggtaacgtt atttccggcc gtattgctaa ccgcttcgac cttggtggca 120

tgaactgtgt cgttgatgca gcatgtgcag gccctcttgc tgcattgcgt atggcattaa 180

gcgagcttgt tgaaggccgc agcgaaatga tgattacagg tggtgtgtgt accgataact 240

caccaaccat gtacatgagc ttctctaaaa caccggcatt cacgacaaac gaaacaattc 300

aaccattcga tattgactcg aaaggtatga tgattggtga aggtatcggt atgattgcgc 360

ttaaacgtct tgaagacgca gagcgtgatg gcgaccgtat ctattccgtg attaaaggtg 420

ttgggtgcat cttcagacgg taatttatta agagtantta tgcgcntcgt cctgaaggtc 480

aggctaaggc acttaaacgt gcttacgacg atgcaggttt cgcaccgcac acacttggct 540

tacttgaagc ccacggcaca ggcacagcag caggtgatgt ggcagaattc agtggtctta 600

actctgtatt cagtgaaggc aatgacgaaa agcaacacat cgcattaggt tcagtgaaat 660

cacagattgg tcacactaaa tcaacagcgg gtactgcggg tctaatcaaa gcgtctttag 720

cactgcacca taaagtactg ccgccaacaa tcaatgtaac cagccctaac cctaaactga 780

atattgaaga ctcgcctttc tacctcaata cacagacgcg tccatggatg caacgtgtcg 840

atggtacacc gcgtcgtgct ggtattagct catttggttt tggtg 885

17

409

Shewanella putrefaciens

17

ccaagctaaa gcacttaacc gtgcttatga agatgccggt tttgcccctg aaacatgtgg 60

tctaattgaa ggccatggta cgggtaccaa agcgggtgat gccgcagaat ttgctggctt 120

gaccaaacac tttggcgccg ccagtgatga aaagcaatat atcgccttag gctcagttaa 180

atcgcaaatt ggtcatacta aatctgcggc tggctctgcg ggtatgatta aggcggcatt 240

agcgctgcat cataaaatct tacctgcaac gatccatatc gataaaccaa gtgaagcctt 300

ggatatcaaa aacagcccgt tatacctaaa cagcgaaacg cgtccttgga tgccacgtga 360

agatggtatt ccacgtcgtg caggtattag ctcatttggt tttggtggc 409

18

81

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

18

ccaagctaaa gcacttaacc gtgcctatga tgatgccggt tttgcccctg aaacatgtgg 60

tctaattgaa ggccatggta c 81

19

81

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

19

ccaagctaaa gcacttaacc gtgcttatga agatgccggt tttgcccctg aaacatgtgg 60

tctaattgaa ggccatggta c 81

20

43

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

20

agaacgcaaa gttgccgcac tgtttggtcg ccaaggttca caa 43

21

43

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

21

caaagcgggt gatgccgcac tgtttggtcg cttgacctaa cac 43

22

55

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

22

cattgcgcta ggttcagtta aatcacaaat tggtcatact aaatcaactg caggt 55

23

55

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

23

tatcgcctta ggctcagtta aatcgcaaat tggtcatact aaatctgcgg ctggc 55

24

29

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

24

cggcttcgat tttggcggca tgaacggtg 29

25

29

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

25

cgcgtatgat taaggcggca ttagcgctg 29

26

28

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

26

gcactgctgc aagcatgaac gcgtcgtt 28

27

28

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

27

gctctgcggc tatcattaac gcggcatt 28

28

29

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

28

tccctggtgc taaccatatc agcaaacca 29

29

29

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

29

tacctgcaac gatccatatc gataaacca 29

30

98

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

30

ctcacctttg tatctaaaca ctgagacttc gtccatggtt accacgtgtt gatggtacgc 60

cgcgccgcgc gggtattagc tcatttggtt ttggtggc 98

31

98

Artificial Sequence

Description of Artificial Sequence SYNTHETIC

31

cagcccgtta tacctaaaca gcgaaacggc gtccttggat gccacgtgaa gatggtattc 60

cacgtcgtgc aggtattagc tcatttggtt ttggtggc 98

32

4

Shewanella putrefaciens

32

Asp Xaa Ala Cys

1

33

4

Shewanella putrefaciens

33

Gly Phe Gly Gly

1

34

5

Shewanella putrefaciens

34

Gly His Ser Xaa Gly

1 5

35

6

Shewanella putrefaciens

35

Leu Gly Xaa Asp Ser Leu

1 5

36

6

Shewanella putrefaciens

36

Leu Gly Xaa Asp Ser Ile

1 5

37

6

Shewanella putrefaciens

37

Gly Xaa Gly Xaa Xaa Gly

1 5

38

6

Shewanella putrefaciens

38

Gly Xaa Gly Xaa Xaa Ala

1 5

39

6

′Axial Seamount′ polynoid polychaete

39

Gly Xaa Gly Xaa Xaa Pro

1 5

40

5

Shewanella putrefaciens

40

Gly Xaa Ser Xaa Gly

1 5

41

35

Artificial Sequence

Description of Artificial Sequence synthetic

41

cuacuacuac uaccaagcta aagcacttaa ccgtg 35

42

32

Artificial Sequence

Description of Artificial Sequence synthetic

42

cuacuacuac uaacagcgaa atgcttatca ag 32

43

38

Artificial Sequence

Description of Artificial Sequence synthetic

43

cuacuacuac uagcgaccaa aaccaaatga gctaatac 38

44

12

Artificial Sequence

Description of Artificial Sequence synthetic

44

aagcccgggc tt 12

45

20

Artificial Sequence

Description of Artificial Sequence synthetic

45

gtacaagccc gggcttagct 20

46

56

Artificial Sequence

Description of Artificial Sequence synthetic

46

cgcgatttaa atggcgcgcc ctgcaggcgg ccgcctgcag ggcgcgccat ttaaat 56

47

41

Artificial Sequence

Description of Artificial Sequence synthetic

47

ctgcagctcg agacaatgtt gatttcctta tacttctgtc c 41

48

37

Artificial Sequence

Description of Artificial Sequence synthetic

48

ggatccagat ctctagctag tcttagctga agctcga 37

49

39

Artificial Sequence

Description of Artificial Sequence synthetic

49

tctagactcg agacaatgag ccagacctct aaacctaca 39

50

37

Artificial Sequence

Description of Artificial Sequence synthetic

50

cccgggctcg agctaattcg cctcactgtc gtttgct 37

51

39

Artificial Sequence

Description of Artificial Sequence synthetic

51

gaattcctcg agacaatgcc gctgcgcatc gcacttatc 39

52

37

Artificial Sequence

Description of Artificial Sequence synthetic

52

ggtaccagat ctttagactt ccccttgaag taaatgg 37

53

39

Artificial Sequence

Description of Artificial Sequence synthetic

53

gaattcgtcg acacaatgtc attaccagac aatgcttct 39

54

38

Artificial Sequence

Description of Artificial Sequence synthetic

54

tctagagtcg acttatacag attcttcgat gctgatag 38

55

39

Artificial Sequence

Description of Artificial Sequence synthetic

55

gaattcgtcg acacaatgaa tcctacagca actaacgaa 39

56

37

Artificial Sequence

Description of Artificial Sequence synthetic

56

tctagaggat ccttaggcca ttctttggtt tggcttc 37

57

39

Artificial Sequence

Description of Artificial Sequence synthetic

57

tctagagtcg acacaatggc ggaattagct gttattggt 39

58

36

Artificial Sequence

Description of Artificial Sequence synthetic

58

gtcgacggat ccctatttgt tcgtgtttgc tatatg 36

59

42

Artificial Sequence

Description of Artificial Sequence synthetic

59

gtcgacggat ccacaatgaa tatagtaagt aatcattcgg ca 42

60

37

Artificial Sequence

Description of Artificial Sequence synthetic

60

gtcgacctcg agttaatcac tcgtacgata acttgcc 37

61

39

Artificial Sequence

Description of Artificial Sequence synthetic

61

cccgggtcga cacaatggct aaaaagaaca ccacatcga 39

62

40

Artificial Sequence

Description of Artificial Sequence synthetic

62

cccgggtcga ctcatgacat atcgttcaaa atgtcactga 40

63

44

Artificial Sequence

Description of Artificial Sequence synthetic

63

tcgacatgga aaatattgca gtagtaggta ttgctaattt gttc 44

64

44

Artificial Sequence

Description of Artificial Sequence synthetic

64

ccgggaacaa attagcaata cctactactg caatattttc catg 44

65

21

Artificial Sequence

Description of Artificial Sequence synthetic

65

tcagatgaac tttatcgata c 21

66

36

Artificial Sequence

Description of Artificial Sequence synthetic

66

tcatgagacg tcgtcgactt acgcttcaac aatact 36

67

30

Schizochytrium aggregatum

67

gtgatgatct ttccctgatg cacgccaagg 30

68

30

Schizochytrium aggregatum

68

agctcgagac cggcaacccg cagcgccaga 30

69

4446

Schizochytrium aggregatum

69

cgctgccgcc gcgtctcgcc gcgccgcgcc gcgccgccgc cgccgctcgc gcgcacgccc 60

gcgcgtctcg ccgcgcctgc tgtctcgaac gagcttctcg agaaggccga gaccgtcgtc 120

atggaggtcc tcgccgccaa gactggctac gagactgaca tgatcgagtc cgacatggag 180

ctcgagactg agctcggcat tgactccatc aagcgtgtcg agatcctctc cgaggttcag 240

gccatgctca acgtcgaggc caaggacgtc gacgctctca gccgcactcg cactgtgggt 300

gaggtcgtca acgccatgaa ggctgagatc gctggtggct ctgccccggc gcctgccgcc 360

gctgccccag gtccggctgc tgccgcccct gcgcctgctg tctcgagcga gcttctcgag 420

aaggccgaga ctgtcgtcat ggaggtcctc gccgccaaga ctggctacga gactgacatg 480

attgagtccg acatggagct cgagaccgag ctcggcattg actccatcaa gcgtgtcgag 540

attctctccg aggttcaggc catgctcaac gtcgaggcca aggacgtcga cgctctcagc 600

cgcactcgca ctgttggtga ggtcgtcgat gccatgaagg ctgagatcgc tggcagctcc 660

gcctcggcgc ctgccgccgc tgctcctgct ccggctgctg ccgctcctgc gcccgctgcc 720

gccgcccctg ctgtctcgaa cgagcttctc gagaaagccg agactgtcgt catggaggtc 780

ctcgccgcca agactggcta cgagactgac atgatcgagt ccgacatgga gctcgagact 840

gagctcggca ttgactccat caagcgtgtc gagatcctct ccgaggttca ggccatgctc 900

aacgtcgagg ccaaggacgt cgatgccctc agccgcaccc gcactgttgg cgaggttgtc 960

gatgccatga aggccgagat cgctggtggc tctgccccgg cgcctgccgc cgctgcccct 1020

gctccggctg ccgccgcccc tgctgtctcg aacgagcttc ttgagaaggc cgagactgtc 1080

gtcatggagg tcctcgccgc caagactggc tacgagaccg acatgatcga gtccgacatg 1140

gagctcgaga ccgagctcgg cattgactcc atcaagcgtg tcgagattct ctccgaggtt 1200

caggccatgc tcaacgtcga ggccaaggac gtcgatgctc tcagccgcac tcgcactgtt 1260

ggcgaggtcg tcgatgccat gaaggctgag atcgccggca gctccgcccc ggcgcctgcc 1320

gccgctgctc ctgctccggc tgctgccgct cctgcgcccg ctgccgctgc ccctgctgtc 1380

tcgagcgagc ttctcgagaa ggccgagacc gtcgtcatgg aggtcctcgc cgccaagact 1440

ggctacgaga ctgacatgat tgagtccgac atggagctcg agactgagct cggcattgac 1500

tccatcaagc gtgtcgagat cctctccgag gttcaggcca tgctcaacgt cgaggccaag 1560

gacgtcgatg ccctcagccg cacccgcact gttggcgagg ttgtcgatgc catgaaggcc 1620

gagatcgctg gtggctctgc cccggcgcct gccgccgctg cccctgctcc ggctgccgcc 1680

gcccctgctg tctcgaacga gcttcttgag aaggccgaga ccgtcgtcat ggaggtcctc 1740

gccgccaaga ctggctacga gaccgacatg atcgagtccg acatggagct cgagaccgag 1800

ctcggcattg actccatcaa gcgtgtcgag attctctccg aggttcaggc catgctcaac 1860

gtcgaggcca aggacgtcga cgctctcagc cgcactcgca ctgttggcga ggtcgtcgat 1920

gccatgaagg ctgagatcgc tggtggctct gccccggcgc ctgccgccgc tgctcctgcc 1980

tcggctggcg ccgcgcctgc ggtcaagatt gactcggtcc acggcgctga ctgtgatgat 2040

ctttccctga tgcacgccaa ggtggttgac atccgccgcc cggacgagct catcctggag 2100

cgccccgaga accgccccgt tctcgttgtc gatgacggca gcgagctcac cctcgccctg 2160

gtccgcgtcc tcggcgcctg cgccgttgtc ctgacctttg agggtctcca gctcgctcag 2220

cgcgctggtg ccgctgccat ccgccacgtg ctcgccaagg atctttccgc ggagagcgcc 2280

gagaaggcca tcaaggaggc cgagcagcgc tttggcgctc tcggcggctt catctcgcag 2340

caggcggagc gcttcgagcc cgccgaaatc ctcggcttca cgctcatgtg cgccaagttc 2400

gccaaggctt ccctctgcac ggctgtggct ggcggccgcc cggcctttat cggtgtggcg 2460

cgccttgacg gccgcctcgg attcacttcg cagggcactt ctgacgcgct caagcgtgcc 2520

cagcgtggtg ccatctttgg cctctgcaag accatcggcc tcgagtggtc cgagtctgac 2580

gtcttttccc gcggcgtgga cattgctcag ggcatgcacc ccgaggatgc cgccgtggcg 2640

attgtgcgcg agatggcgtg cgctgacatt cgcattcgcg aggtcggcat tggcgcaaac 2700

cagcagcgct gcacgatccg tgccgccaag ctcgagaccg gcaacccgca gcgccagatc 2760

gccaaggacg acgtgctgct cgtttctggc ggcgctcgcg gcatcacgcc tctttgcatc 2820

cgggagatca cgcgccagat cgcgggcggc aagtacattc tgcttggccg cagcaaggtc 2880

tctgcgagcg aaccggcatg gtgcgctggc atcactgacg agaaggctgt gcaaaaggct 2940

gctacccagg agctcaagcg cgcctttagc gctggcgagg gccccaagcc cacgccccgc 3000

gctgtcacta agcttgtggg ctctgttctt ggcgctcgcg aggtgcgcag ctctattgct 3060

gcgattgaag cgctcggcgg caaggccatc tactcgtcgt gcgacgtgaa ctctgccgcc 3120

gacgtggcca aggccgtgcg cgatgccgag tcccagctcg gtgcccgcgt ctcgggcatc 3180

gttcatgcct cgggcgtgct ccgcgaccgt ctcatcgaga agaagctccc cgacgagttc 3240

gacgccgtct ttggcaccaa ggtcaccggt ctcgagaacc tcctcgccgc cgtcgaccgc 3300

gccaacctca agcacatggt cctcttcagc tcgctcgccg gcttccacgg caacgtcggc 3360

cagtctgact acgccatggc caacgaggcc cttaacaaga tgggcctcga gctcgccaag 3420

gacgtctcgg tcaagtcgat ctgcttcggt ccctgggacg gtggcatggt gacgccgcag 3480

ctcaagaagc agttccagga gatgggcgtg cagatcatcc cccgcgaggg cggcgctgat 3540

accgtggcgc gcatcgtgct cggctcctcg ccggctgaga tccttgtcgg caactggcgc 3600

accccgtcca agaaggtcgg ctcggacacc atcaccctgc accgcaagat ttccgccaag 3660

tccaacccct tcctcgagga ccacgtcatc cagggccgcc gcgtgctgcc catgacgctg 3720

gccattggct cgctcgcgga gacctgcctc ggcctcttcc ccggctactc gctctgggcc 3780

attgacgacg cccagctctt caagggtgtc actgtcgacg gcgacgtcaa ctgcgaggtg 3840

accctcaccc cgtcgacggc gccctcgggc cgcgtcaacg tccaggccac gctcaagacc 3900

ttttccagcg gcaagctggt cccggcctac cgcgccgtca tcgtgctctc caaccagggc 3960

gcgcccccgg ccaacgccac catgcagccg ccctcgctcg atgccgatcc ggcgctccag 4020

ggctccgtct acgacggcaa gaccctcttc cacggcccgg ccttccgcgg catcgatgac 4080

gtgctctcgt gcaccaagag ccagcttgtg gccaagtgca gcgctgtccc cggctccgac 4140

gccgctcgcg gcgagtttgc cacggacact gacgcccatg accccttcgt gaacgacctg 4200

gcctttcagg ccatgctcgt ctgggtgcgc cgcacgctcg gccaggctgc gctccccaac 4260

tcgatccagc gcatcgtcca gcaccgcccg gtcccgcagg acaagccctt ctacattacc 4320

ctccgctcca accagtcggg cggtcactcc cagcacaagc acgcccttca gttccacaac 4380

gagcagggcg atctcttcat tgatgtccag gcttcggtca tcgccacgga cagccttgcc 4440

ttctaa 4446

70

1481

Schizochytrium aggregatum

70

Arg Cys Arg Arg Val Ser Pro Arg Arg Ala Ala Pro Pro Pro Pro Leu

1 5 10 15

Ala Arg Thr Pro Ala Arg Leu Ala Ala Pro Ala Val Ser Asn Glu Leu

20 25 30

Leu Glu Lys Ala Glu Thr Val Val Met Glu Val Leu Ala Ala Lys Thr

35 40 45

Gly Tyr Glu Thr Asp Met Ile Glu Ser Asp Met Glu Leu Glu Thr Glu

50 55 60

Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Ser Glu Val Gln

65 70 75 80

Ala Met Leu Asn Val Glu Ala Lys Asp Val Asp Ala Leu Ser Arg Thr

85 90 95

Arg Thr Val Gly Glu Val Val Asn Ala Met Lys Ala Glu Ile Ala Gly

100 105 110

Gly Ser Ala Pro Ala Pro Ala Ala Ala Ala Pro Gly Pro Ala Ala Ala

115 120 125

Ala Pro Ala Pro Ala Val Ser Ser Glu Leu Leu Glu Lys Ala Glu Thr

130 135 140

Val Val Met Glu Val Leu Ala Ala Lys Thr Gly Tyr Glu Thr Asp Met

145 150 155 160

Ile Glu Ser Asp Met Glu Leu Glu Thr Glu Leu Gly Ile Asp Ser Ile

165 170 175

Lys Arg Val Glu Ile Leu Ser Glu Val Gln Ala Met Leu Asn Val Glu

180 185 190

Ala Lys Asp Val Asp Ala Leu Ser Arg Thr Arg Thr Val Gly Glu Val

195 200 205

Val Asp Ala Met Lys Ala Glu Ile Ala Gly Ser Ser Ala Ser Ala Pro

210 215 220

Ala Ala Ala Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Ala

225 230 235 240

Ala Ala Pro Ala Val Ser Asn Glu Leu Leu Glu Lys Ala Glu Thr Val

245 250 255

Val Met Glu Val Leu Ala Ala Lys Thr Gly Tyr Glu Thr Asp Met Ile

260 265 270

Glu Ser Asp Met Glu Leu Glu Thr Glu Leu Gly Ile Asp Ser Ile Lys

275 280 285

Arg Val Glu Ile Leu Ser Glu Val Gln Ala Met Leu Asn Val Glu Ala

290 295 300

Lys Asp Val Asp Ala Leu Ser Arg Thr Arg Thr Val Gly Glu Val Val

305 310 315 320

Asp Ala Met Lys Ala Glu Ile Ala Gly Gly Ser Ala Pro Ala Pro Ala

325 330 335

Ala Ala Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Val Ser Asn Glu

340 345 350

Leu Leu Glu Lys Ala Glu Thr Val Val Met Glu Val Leu Ala Ala Lys

355 360 365

Thr Gly Tyr Glu Thr Asp Met Ile Glu Ser Asp Met Glu Leu Glu Thr

370 375 380

Glu Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Ser Glu Val

385 390 395 400

Gln Ala Met Leu Asn Val Glu Ala Lys Asp Val Asp Ala Leu Ser Arg

405 410 415

Thr Arg Thr Val Gly Glu Val Val Asp Ala Met Lys Ala Glu Ile Ala

420 425 430

Gly Ser Ser Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Ala

435 440 445

Ala Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Val Ser Ser Glu Leu

450 455 460

Leu Glu Lys Ala Glu Thr Val Val Met Glu Val Leu Ala Ala Lys Thr

465 470 475 480

Gly Tyr Glu Thr Asp Met Ile Glu Ser Asp Met Glu Leu Glu Thr Glu

485 490 495

Leu Gly Ile Asp Ser Ile Lys Arg Val Glu Ile Leu Ser Glu Val Gln

500 505 510

Ala Met Leu Asn Val Glu Ala Lys Asp Val Asp Ala Leu Ser Arg Thr

515 520 525

Arg Thr Val Gly Glu Val Val Asp Ala Met Lys Ala Glu Ile Ala Gly

530 535 540

Gly Ser Ala Pro Ala Pro Ala Ala Ala Ala Pro Ala Pro Ala Ala Ala

545 550 555 560

Ala Pro Ala Val Ser Asn Glu Leu Leu Glu Lys Ala Glu Thr Val Val

565 570 575

Met Glu Val Leu Ala Ala Lys Thr Gly Tyr Glu Thr Asp Met Ile Glu

580 585 590

Ser Asp Met Glu Leu Glu Thr Glu Leu Gly Ile Asp Ser Ile Lys Arg

595 600 605

Val Glu Ile Leu Ser Glu Val Gln Ala Met Leu Asn Val Glu Ala Lys

610 615 620

Asp Val Asp Ala Leu Ser Arg Thr Arg Thr Val Gly Glu Val Val Asp

625 630 635 640

Ala Met Lys Ala Glu Ile Ala Gly Gly Ser Ala Pro Ala Pro Ala Ala

645 650 655

Ala Ala Pro Ala Ser Ala Gly Ala Ala Pro Ala Val Lys Ile Asp Ser

660 665 670

Val His Gly Ala Asp Cys Asp Asp Leu Ser Leu Met His Ala Lys Val

675 680 685

Val Asp Ile Arg Arg Pro Asp Glu Leu Ile Leu Glu Arg Pro Glu Asn

690 695 700

Arg Pro Val Leu Val Val Asp Asp Gly Ser Glu Leu Thr Leu Ala Leu

705 710 715 720

Val Arg Val Leu Gly Ala Cys Ala Val Val Leu Thr Phe Glu Gly Leu

725 730 735

Gln Leu Ala Gln Arg Ala Gly Ala Ala Ala Ile Arg His Val Leu Ala

740 745 750

Lys Asp Leu Ser Ala Glu Ser Ala Glu Lys Ala Ile Lys Glu Ala Glu

755 760 765

Gln Arg Phe Gly Ala Leu Gly Gly Phe Ile Ser Gln Gln Ala Glu Arg

770 775 780

Phe Glu Pro Ala Glu Ile Leu Gly Phe Thr Leu Met Cys Ala Lys Phe

785 790 795 800

Ala Lys Ala Ser Leu Cys Thr Ala Val Ala Gly Gly Arg Pro Ala Phe

805 810 815

Ile Gly Val Ala Arg Leu Asp Gly Arg Leu Gly Phe Thr Ser Gln Gly

820 825 830

Thr Ser Asp Ala Leu Lys Arg Ala Gln Arg Gly Ala Ile Phe Gly Leu

835 840 845

Cys Lys Thr Ile Gly Leu Glu Trp Ser Glu Ser Asp Val Phe Ser Arg

850 855 860

Gly Val Asp Ile Ala Gln Gly Met His Pro Glu Asp Ala Ala Val Ala

865 870 875 880

Ile Val Arg Glu Met Ala Cys Ala Asp Ile Arg Ile Arg Glu Val Gly

885 890 895

Ile Gly Ala Asn Gln Gln Arg Cys Thr Ile Arg Ala Ala Lys Leu Glu

900 905 910

Thr Gly Asn Pro Gln Arg Gln Ile Ala Lys Asp Asp Val Leu Leu Val

915 920 925

Ser Gly Gly Ala Arg Gly Ile Thr Pro Leu Cys Ile Arg Glu Ile Thr

930 935 940

Arg Gln Ile Ala Gly Gly Lys Tyr Ile Leu Leu Gly Arg Ser Lys Val

945 950 955 960

Ser Ala Ser Glu Pro Ala Trp Cys Ala Gly Ile Thr Asp Glu Lys Ala

965 970 975

Val Gln Lys Ala Ala Thr Gln Glu Leu Lys Arg Ala Phe Ser Ala Gly

980 985 990

Glu Gly Pro Lys Pro Thr Pro Arg Ala Val Thr Lys Leu Val Gly Ser

995 1000 1005

Val Leu Gly Ala Arg Glu Val Arg Ser Ser Ile Ala Ala Ile Glu Ala

1010 1015 1020

Leu Gly Gly Lys Ala Ile Tyr Ser Ser Cys Asp Val Asn Ser Ala Ala

1025 1030 1035 1040

Asp Val Ala Lys Ala Val Arg Asp Ala Glu Ser Gln Leu Gly Ala Arg

1045 1050 1055

Val Ser Gly Ile Val His Ala Ser Gly Val Leu Arg Asp Arg Leu Ile

1060 1065 1070

Glu Lys Lys Leu Pro Asp Glu Phe Asp Ala Val Phe Gly Thr Lys Val

1075 1080 1085

Thr Gly Leu Glu Asn Leu Leu Ala Ala Val Asp Arg Ala Asn Leu Lys

1090 1095 1100

His Met Val Leu Phe Ser Ser Leu Ala Gly Phe His Gly Asn Val Gly

1105 1110 1115 1120

Gln Ser Asp Tyr Ala Met Ala Asn Glu Ala Leu Asn Lys Met Gly Leu

1125 1130 1135

Glu Leu Ala Lys Asp Val Ser Val Lys Ser Ile Cys Phe Gly Pro Trp

1140 1145 1150

Asp Gly Gly Met Val Thr Pro Gln Leu Lys Lys Gln Phe Gln Glu Met

1155 1160 1165

Gly Val Gln Ile Ile Pro Arg Glu Gly Gly Ala Asp Thr Val Ala Arg

1170 1175 1180

Ile Val Leu Gly Ser Ser Pro Ala Glu Ile Leu Val Gly Asn Trp Arg

1185 1190 1195 1200

Thr Pro Ser Lys Lys Val Gly Ser Asp Thr Ile Thr Leu His Arg Lys

1205 1210 1215

Ile Ser Ala Lys Ser Asn Pro Phe Leu Glu Asp His Val Ile Gln Gly

1220 1225 1230

Arg Arg Val Leu Pro Met Thr Leu Ala Ile Gly Ser Leu Ala Glu Thr

1235 1240 1245

Cys Leu Gly Leu Phe Pro Gly Tyr Ser Leu Trp Ala Ile Asp Asp Ala

1250 1255 1260

Gln Leu Phe Lys Gly Val Thr Val Asp Gly Asp Val Asn Cys Glu Val

1265 1270 1275 1280

Thr Leu Thr Pro Ser Thr Ala Pro Ser Gly Arg Val Asn Val Gln Ala

1285 1290 1295

Thr Leu Lys Thr Phe Ser Ser Gly Lys Leu Val Pro Ala Tyr Arg Ala

1300 1305 1310

Val Ile Val Leu Ser Asn Gln Gly Ala Pro Pro Ala Asn Ala Thr Met

1315 1320 1325

Gln Pro Pro Ser Leu Asp Ala Asp Pro Ala Leu Gln Gly Ser Val Tyr

1330 1335 1340

Asp Gly Lys Thr Leu Phe His Gly Pro Ala Phe Arg Gly Ile Asp Asp

1345 1350 1355 1360

Val Leu Ser Cys Thr Lys Ser Gln Leu Val Ala Lys Cys Ser Ala Val

1365 1370 1375

Pro Gly Ser Asp Ala Ala Arg Gly Glu Phe Ala Thr Asp Thr Asp Ala

1380 1385 1390

His Asp Pro Phe Val Asn Asp Leu Ala Phe Gln Ala Met Leu Val Trp

1395 1400 1405

Val Arg Arg Thr Leu Gly Gln Ala Ala Leu Pro Asn Ser Ile Gln Arg

1410 1415 1420

Ile Val Gln His Arg Pro Val Pro Gln Asp Lys Pro Phe Tyr Ile Thr

1425 1430 1435 1440

Leu Arg Ser Asn Gln Ser Gly Gly His Ser Gln His Lys His Ala Leu

1445 1450 1455

Gln Phe His Asn Glu Gln Gly Asp Leu Phe Ile Asp Val Gln Ala Ser

1460 1465 1470

Val Ile Ala Thr Asp Ser Leu Ala Phe

1475 1480

71

5215

Schizochytrium aggregatum

71

tgccgtcttt gaggagcatg acccctccaa cgccgcctgc acgggccacg actccatttc 60

tgcgctctcg gcccgctgcg gcggtgaaag caacatgcgc atcgccatca ctggtatgga 120

cgccaccttt ggcgctctca agggactcga cgccttcgag cgcgccattt acaccggcgc 180

tcacggtgcc atcccactcc cagaaaagcg ctggcgcttt ctcggcaagg acaaggactt 240

tcttgacctc tgcggcgtca aggccacccc gcacggctgc tacattgaag atgttgaggt 300

cgacttccag cgcctccgca cgcccatgac ccctgaagac atgctcctcc ctcagcagct 360

tctggccgtc accaccattg accgcgccat cctcgactcg ggaatgaaaa agggtggcaa 420

tgtcgccgtc tttgtcggcc tcggcaccga cctcgagctc taccgtcacc gtgctcgcgt 480

cgctctcaag gagcgcgtcc gccctgaagc ctccaagaag ctcaatgaca tgatgcagta 540

cattaacgac tgcggcacat ccacatcgta cacctcgtac attggcaacc tcgtcgccac 600

gcgcgtctcg tcgcagtggg gcttcacggg cccctccttt acgatcaccg agggcaacaa 660

ctccgtctac cgctgcgccg agctcggcaa gtacctcctc gagaccggcg aggtcgatgg 720

cgtcgtcgtt gcgggtgtcg atctctgcgg cagtgccgaa aacctttacg tcaagtctcg 780

ccgcttcaag gtgtccacct ccgatacccc gcgcgccagc tttgacgccg ccgccgatgg 840

ctactttgtc ggcgagggct gcggtgcctt tgtgctcaag cgtgagacta gctgcaccaa 900

ggacgaccgt atctacgctt gcatggatgc catcgtccct ggcaacgtcc ctagcgcctg 960

cttgcgcgag gccctcgacc aggcgcgcgt caagccgggc gatatcgaga tgctcgagct 1020

cagcgccgac tccgcccgcc acctcaagga cccgtccgtc ctgcccaagg agctcactgc 1080

cgaggaggaa atcggcggcc ttcagacgat ccttcgtgac gatgacaagc tcccgcgcaa 1140

cgtcgcaacg ggcagtgtca aggccaccgt cggtgacacc ggttatgcct ctggtgctgc 1200

cagcctcatc aaggctgcgc tttgcatcta caaccgctac ctgcccagca acggcgacga 1260

ctgggatgaa cccgcccctg aggcgccctg ggacagcacc ctctttgcgt gccagacctc 1320

gcgcgcttgg ctcaagaacc ctggcgagcg tcgctatgcg gccgtctcgg gcgtctccga 1380

gacgcgctcg tgctattccg tgctcctctc cgaagccgag ggccactacg agcgcgagaa 1440

ccgcatctcg ctcgacgagg aggcgcccaa gctcattgtg cttcgcgccg actcccacga 1500

ggagatcctt ggtcgcctcg acaagatccg cgagcgcttc ttgcagccca cgggcgccgc 1560

cccgcgcgag tccgagctca aggcgcaggc ccgccgcatc ttcctcgagc tcctcggcga 1620

gacccttgcc caggatgccg cttcttcagg ctcgcaaaag cccctcgctc tcagcctcgt 1680

ctccacgccc tccaagctcc agcgcgaggt cgagctcgcg gccaagggta tcccgcgctg 1740

cctcaagatg cgccgcgatt ggagctcccc tgctggcagc cgctacgcgc ctgagccgct 1800

cgccagcgac cgcgtcgcct tcatgtacgg cgaaggtcgc agcccttact acggcatcac 1860

ccaagacatt caccgcattt ggcccgaact ccacgaggtc atcaacgaaa agacgaaccg 1920

tctctgggcc gaaggcgacc gctgggtcat gccgcgcgcc agcttcaagt cggagctcga 1980

gagccagcag caagagtttg atcgcaacat gattgaaatg ttccgtcttg gaatcctcac 2040

ctcaattgcc ttcaccaatc tggcgcgcga cgttctcaac atcacgccca aggccgcctt 2100

tggcctcagt cttggcgaga tttccatgat ttttgccttt tccaagaaga acggtctcat 2160

ctccgaccag ctcaccaagg atcttcgcga gtccgacgtg tggaacaagg ctctggccgt 2220

tgaatttaat gcgctgcgcg aggcctgggg cattccacag agtgtcccca aggacgagtt 2280

ctggcaaggc tacattgtgc gcggcaccaa gcaggatatc gaggcggcca tcgccccgga 2340

cagcaagtac gtgcgcctca ccatcatcaa tgatgccaac accgccctca ttagcggcaa 2400

gcccgacgcc tgcaaggctg cgatcgcgcg tctcggtggc aacattcctg cgcttcccgt 2460

gacccagggc atgtgcggcc actgccccga ggtgggacct tataccaagg atatcgccaa 2520

gatccatgcc aaccttgagt tccccgttgt cgacggcctt gacctctgga ccacaatcaa 2580

ccagaagcgc ctcgtgccac gcgccacggg cgccaaggac gaatgggccc cttcttcctt 2640

tggcgagtac gccggccagc tctacgagaa gcaggctaac ttcccccaaa tcgtcgagac 2700

catttacaag caaaactacg acgtctttgt cgaggttggg cccaacaacc accgtagcac 2760

cgcagtgcgc accacgcttg gtccccagcg caaccacctt gctggcgcca tcgacaagca 2820

gaacgaggat gcttggacga ccatcgtcaa gcttgtggct tcgctcaagg cccaccttgt 2880

tcctggcgtc acgatctcgc cgctgtacca ctccaagctt gtggcggagg ctcaggcttg 2940

ctacgctgcg ctctgcaagg gtgaaaagcc caagaagaac aagtttgtgc gcaagattca 3000

gctcaacggt cgcttcaaca gcaaggcgga ccccatctcc tcggccgatc ttgccagctt 3060

tccgcctgcg gaccctgcca ttgaagccgc catctcgagc cgcatcatga agcctgtcgc 3120

tcccaagttc tacgcgcgtc tcaacattga cgagcaggac gagacccgag atccgatcct 3180

caacaaggac aacgcgccgt cttcttcttc ttcttcttct tcttcttctt cttcttcttc 3240

ttctccgtcg cctgctcctt cggcccccgt gcaaaagaag gctgctcccg ccgcggagac 3300

caaggctgtt gcttcggctg acgcacttcg cagtgccctg ctcgatctcg acagtatgct 3360

tgcgctgagc tctgccagtg cctccggcaa ccttgttgag actgcgccta gcgacgcctc 3420

ggtcattgtg ccgccctgca acattgcgga tctcggcagc cgcgccttca tgaaaacgta 3480

cggtgtttcg gcgcctctgt acacgggcgc catggccaag ggcattgcct ctgcggacct 3540

cgtcattgcc gccggccgcc agggcatcct tgcgtccttt ggcgccggcg gacttcccat 3600

gcaggttgtg cgtgagtcca tcgaaaagat tcaggccgcc ctgcccaatg gcccgtacgc 3660

tgtcaacctt atccattctc cctttgacag caacctcgaa aagggcaatg tcgatctctt 3720

cctcgagaag ggtgtcacct ttgtcgaggc ctcggccttt atgacgctca ccccgcaggt 3780

cgtgcggtac cgcgcggctg gcctcacgcg caacgccgac ggctcggtca acatccgcaa 3840

ccgtatcatt ggcaaggtct cgcgcaccga gctcgccgag atgttcatgc gtcctgcgcc 3900

cgagcacctt cttcagaagc tcattgcttc cggcgagatc aaccaggagc aggccgagct 3960

cgcccgccgt gttcccgtcg ctgacgacat cgcggtcgaa gctgactcgg gtggccacac 4020

cgacaaccgc cccatccacg tcattctgcc cctcatcatc aaccttcgcg accgccttca 4080

ccgcgagtgc ggctacccgg ccaaccttcg cgtccgtgtg ggcgccggcg gtggcattgg 4140

gtgcccccag gcggcgctgg ccaccttcaa catgggtgcc tcctttattg tcaccggcac 4200

cgtgaaccag gtcgccaagc agtcgggcac gtgcgacaat gtgcgcaagc agctcgcgaa 4260

ggccacttac tcggacgtat gcatggcccc ggctgccgac atgttcgagg aaggcgtcaa 4320

gcttcaggtc ctcaagaagg gaaccatgtt tccctcgcgc gccaacaagc tctacgagct 4380

cttttgcaag tacgactcgt tcgagtccat gccccccgca gagcttgcgc gcgtcgagaa 4440

gcgcatcttc agccgcgcgc tcgaagaggt ctgggacgag accaaaaact tttacattaa 4500

ccgtcttcac aacccggaga agatccagcg cgccgagcgc gaccccaagc tcaagatgtc 4560

gctgtgcttt cgctggtacc tgagcctggc gagccgctgg gccaacactg gagcttccga 4620

tcgcgtcatg gactaccagg tctggtgcgg tcctgccatt ggttccttca acgatttcat 4680

caagggaact taccttgatc cggccgtcgc aaacgagtac ccgtgcgtcg ttcagattaa 4740

caagcagatc cttcgtggag cgtgcttctt gcgccgtctc gaaattctgc gcaacgcacg 4800

cctttccgat ggcgctgccg ctcttgtggc cagcatcgat gacacatacg tcccggccga 4860

gaagctgtaa gtaagctctc atatatgtta gttgcgtgag accgacacga agataatatc 4920

acatacgctt ttgtttgttc tttcaattat ttgtctgtgc ttcatgttgc tcctcagtat 4980

ctagctggcg gctcttatct tcttttaaaa tatctggaca aggacaaaaa caagaataaa 5040

ggcgagaaga tgtgaatttc atttcgactt gagaactcga agagcattga tgcggttagt 5100

atatgggtat tttccagaca cttttcatca tcatcatcat catcatcatt atgaagaagt 5160

agtagctgat aaagtagact cactgtttgc agcgagaaaa aaaaaaaaaa aaaaa 5215

72

1622

Schizochytrium aggregatum

72

Ala Val Phe Glu Glu His Asp Pro Ser Asn Ala Ala Cys Thr Gly His

1 5 10 15

Asp Ser Ile Ser Ala Leu Ser Ala Arg Cys Gly Gly Glu Ser Asn Met

20 25 30

Arg Ile Ala Ile Thr Gly Met Asp Ala Thr Phe Gly Ala Leu Lys Gly

35 40 45

Leu Asp Ala Phe Glu Arg Ala Ile Tyr Thr Gly Ala His Gly Ala Ile

50 55 60

Pro Leu Pro Glu Lys Arg Trp Arg Phe Leu Gly Lys Asp Lys Asp Phe

65 70 75 80

Leu Asp Leu Cys Gly Val Lys Ala Thr Pro His Gly Cys Tyr Ile Glu

85 90 95

Asp Val Glu Val Asp Phe Gln Arg Leu Arg Thr Pro Met Thr Pro Glu

100 105 110

Asp Met Leu Leu Pro Gln Gln Leu Leu Ala Val Thr Thr Ile Asp Arg

115 120 125

Ala Ile Leu Asp Ser Gly Met Lys Lys Gly Gly Asn Val Ala Val Phe

130 135 140

Val Gly Leu Gly Thr Asp Leu Glu Leu Tyr Arg His Arg Ala Arg Val

145 150 155 160

Ala Leu Lys Glu Arg Val Arg Pro Glu Ala Ser Lys Lys Leu Asn Asp

165 170 175

Met Met Gln Tyr Ile Asn Asp Cys Gly Thr Ser Thr Ser Tyr Thr Ser

180 185 190

Tyr Ile Gly Asn Leu Val Ala Thr Arg Val Ser Ser Gln Trp Gly Phe

195 200 205

Thr Gly Pro Ser Phe Thr Ile Thr Glu Gly Asn Asn Ser Val Tyr Arg

210 215 220

Cys Ala Glu Leu Gly Lys Tyr Leu Leu Glu Thr Gly Glu Val Asp Gly

225 230 235 240

Val Val Val Ala Gly Val Asp Leu Cys Gly Ser Ala Glu Asn Leu Tyr

245 250 255

Val Lys Ser Arg Arg Phe Lys Val Ser Thr Ser Asp Thr Pro Arg Ala

260 265 270

Ser Phe Asp Ala Ala Ala Asp Gly Tyr Phe Val Gly Glu Gly Cys Gly

275 280 285

Ala Phe Val Leu Lys Arg Glu Thr Ser Cys Thr Lys Asp Asp Arg Ile

290 295 300

Tyr Ala Cys Met Asp Ala Ile Val Pro Gly Asn Val Pro Ser Ala Cys

305 310 315 320

Leu Arg Glu Ala Leu Asp Gln Ala Arg Val Lys Pro Gly Asp Ile Glu

325 330 335

Met Leu Glu Leu Ser Ala Asp Ser Ala Arg His Leu Lys Asp Pro Ser

340 345 350

Val Leu Pro Lys Glu Leu Thr Ala Glu Glu Glu Ile Gly Gly Leu Gln

355 360 365

Thr Ile Leu Arg Asp Asp Asp Lys Leu Pro Arg Asn Val Ala Thr Gly

370 375 380

Ser Val Lys Ala Thr Val Gly Asp Thr Gly Tyr Ala Ser Gly Ala Ala

385 390 395 400

Ser Leu Ile Lys Ala Ala Leu Cys Ile Tyr Asn Arg Tyr Leu Pro Ser

405 410 415

Asn Gly Asp Asp Trp Asp Glu Pro Ala Pro Glu Ala Pro Trp Asp Ser

420 425 430

Thr Leu Phe Ala Cys Gln Thr Ser Arg Ala Trp Leu Lys Asn Pro Gly

435 440 445

Glu Arg Arg Tyr Ala Ala Val Ser Gly Val Ser Glu Thr Arg Ser Cys

450 455 460

Tyr Ser Val Leu Leu Ser Glu Ala Glu Gly His Tyr Glu Arg Glu Asn

465 470 475 480

Arg Ile Ser Leu Asp Glu Glu Ala Pro Lys Leu Ile Val Leu Arg Ala

485 490 495

Asp Ser His Glu Glu Ile Leu Gly Arg Leu Asp Lys Ile Arg Glu Arg

500 505 510

Phe Leu Gln Pro Thr Gly Ala Ala Pro Arg Glu Ser Glu Leu Lys Ala

515 520 525

Gln Ala Arg Arg Ile Phe Leu Glu Leu Leu Gly Glu Thr Leu Ala Gln

530 535 540

Asp Ala Ala Ser Ser Gly Ser Gln Lys Pro Leu Ala Leu Ser Leu Val

545 550 555 560

Ser Thr Pro Ser Lys Leu Gln Arg Glu Val Glu Leu Ala Ala Lys Gly

565 570 575

Ile Pro Arg Cys Leu Lys Met Arg Arg Asp Trp Ser Ser Pro Ala Gly

580 585 590

Ser Arg Tyr Ala Pro Glu Pro Leu Ala Ser Asp Arg Val Ala Phe Met

595 600 605

Tyr Gly Glu Gly Arg Ser Pro Tyr Tyr Gly Ile Thr Gln Asp Ile His

610 615 620

Arg Ile Trp Pro Glu Leu His Glu Val Ile Asn Glu Lys Thr Asn Arg

625 630 635 640

Leu Trp Ala Glu Gly Asp Arg Trp Val Met Pro Arg Ala Ser Phe Lys

645 650 655

Ser Glu Leu Glu Ser Gln Gln Gln Glu Phe Asp Arg Asn Met Ile Glu

660 665 670

Met Phe Arg Leu Gly Ile Leu Thr Ser Ile Ala Phe Thr Asn Leu Ala

675 680 685

Arg Asp Val Leu Asn Ile Thr Pro Lys Ala Ala Phe Gly Leu Ser Leu

690 695 700

Gly Glu Ile Ser Met Ile Phe Ala Phe Ser Lys Lys Asn Gly Leu Ile

705 710 715 720

Ser Asp Gln Leu Thr Lys Asp Leu Arg Glu Ser Asp Val Trp Asn Lys

725 730 735

Ala Leu Ala Val Glu Phe Asn Ala Leu Arg Glu Ala Trp Gly Ile Pro

740 745 750

Gln Ser Val Pro Lys Asp Glu Phe Trp Gln Gly Tyr Ile Val Arg Gly

755 760 765

Thr Lys Gln Asp Ile Glu Ala Ala Ile Ala Pro Asp Ser Lys Tyr Val

770 775 780

Arg Leu Thr Ile Ile Asn Asp Ala Asn Thr Ala Leu Ile Ser Gly Lys

785 790 795 800

Pro Asp Ala Cys Lys Ala Ala Ile Ala Arg Leu Gly Gly Asn Ile Pro

805 810 815

Ala Leu Pro Val Thr Gln Gly Met Cys Gly His Cys Pro Glu Val Gly

820 825 830

Pro Tyr Thr Lys Asp Ile Ala Lys Ile His Ala Asn Leu Glu Phe Pro

835 840 845

Val Val Asp Gly Leu Asp Leu Trp Thr Thr Ile Asn Gln Lys Arg Leu

850 855 860

Val Pro Arg Ala Thr Gly Ala Lys Asp Glu Trp Ala Pro Ser Ser Phe

865 870 875 880

Gly Glu Tyr Ala Gly Gln Leu Tyr Glu Lys Gln Ala Asn Phe Pro Gln

885 890 895

Ile Val Glu Thr Ile Tyr Lys Gln Asn Tyr Asp Val Phe Val Glu Val

900 905 910

Gly Pro Asn Asn His Arg Ser Thr Ala Val Arg Thr Thr Leu Gly Pro

915 920 925

Gln Arg Asn His Leu Ala Gly Ala Ile Asp Lys Gln Asn Glu Asp Ala

930 935 940

Trp Thr Thr Ile Val Lys Leu Val Ala Ser Leu Lys Ala His Leu Val

945 950 955 960

Pro Gly Val Thr Ile Ser Pro Leu Tyr His Ser Lys Leu Val Ala Glu

965 970 975

Ala Gln Ala Cys Tyr Ala Ala Leu Cys Lys Gly Glu Lys Pro Lys Lys

980 985 990

Asn Lys Phe Val Arg Lys Ile Gln Leu Asn Gly Arg Phe Asn Ser Lys

995 1000 1005

Ala Asp Pro Ile Ser Ser Ala Asp Leu Ala Ser Phe Pro Pro Ala Asp

1010 1015 1020

Pro Ala Ile Glu Ala Ala Ile Ser Ser Arg Ile Met Lys Pro Val Ala

1025 1030 1035 1040

Pro Lys Phe Tyr Ala Arg Leu Asn Ile Asp Glu Gln Asp Glu Thr Arg

1045 1050 1055

Asp Pro Ile Leu Asn Lys Asp Asn Ala Pro Ser Ser Ser Ser Ser Ser

1060 1065 1070

Ser Ser Ser Ser Ser Ser Ser Ser Ser Pro Ser Pro Ala Pro Ser Ala

1075 1080 1085

Pro Val Gln Lys Lys Ala Ala Pro Ala Ala Glu Thr Lys Ala Val Ala

1090 1095 1100

Ser Ala Asp Ala Leu Arg Ser Ala Leu Leu Asp Leu Asp Ser Met Leu

1105 1110 1115 1120

Ala Leu Ser Ser Ala Ser Ala Ser Gly Asn Leu Val Glu Thr Ala Pro

1125 1130 1135

Ser Asp Ala Ser Val Ile Val Pro Pro Cys Asn Ile Ala Asp Leu Gly

1140 1145 1150

Ser Arg Ala Phe Met Lys Thr Tyr Gly Val Ser Ala Pro Leu Tyr Thr

1155 1160 1165

Gly Ala Met Ala Lys Gly Ile Ala Ser Ala Asp Leu Val Ile Ala Ala

1170 1175 1180

Gly Arg Gln Gly Ile Leu Ala Ser Phe Gly Ala Gly Gly Leu Pro Met

1185 1190 1195 1200

Gln Val Val Arg Glu Ser Ile Glu Lys Ile Gln Ala Ala Leu Pro Asn

1205 1210 1215

Gly Pro Tyr Ala Val Asn Leu Ile His Ser Pro Phe Asp Ser Asn Leu

1220 1225 1230

Glu Lys Gly Asn Val Asp Leu Phe Leu Glu Lys Gly Val Thr Phe Val

1235 1240 1245

Glu Ala Ser Ala Phe Met Thr Leu Thr Pro Gln Val Val Arg Tyr Arg

1250 1255 1260

Ala Ala Gly Leu Thr Arg Asn Ala Asp Gly Ser Val Asn Ile Arg Asn

1265 1270 1275 1280

Arg Ile Ile Gly Lys Val Ser Arg Thr Glu Leu Ala Glu Met Phe Met

1285 1290 1295

Arg Pro Ala Pro Glu His Leu Leu Gln Lys Leu Ile Ala Ser Gly Glu

1300 1305 1310

Ile Asn Gln Glu Gln Ala Glu Leu Ala Arg Arg Val Pro Val Ala Asp

1315 1320 1325

Asp Ile Ala Val Glu Ala Asp Ser Gly Gly His Thr Asp Asn Arg Pro

1330 1335 1340

Ile His Val Ile Leu Pro Leu Ile Ile Asn Leu Arg Asp Arg Leu His

1345 1350 1355 1360

Arg Glu Cys Gly Tyr Pro Ala Asn Leu Arg Val Arg Val Gly Ala Gly

1365 1370 1375

Gly Gly Ile Gly Cys Pro Gln Ala Ala Leu Ala Thr Phe Asn Met Gly

1380 1385 1390

Ala Ser Phe Ile Val Thr Gly Thr Val Asn Gln Val Ala Lys Gln Ser

1395 1400 1405

Gly Thr Cys Asp Asn Val Arg Lys Gln Leu Ala Lys Ala Thr Tyr Ser

1410 1415 1420

Asp Val Cys Met Ala Pro Ala Ala Asp Met Phe Glu Glu Gly Val Lys

1425 1430 1435 1440

Leu Gln Val Leu Lys Lys Gly Thr Met Phe Pro Ser Arg Ala Asn Lys

1445 1450 1455

Leu Tyr Glu Leu Phe Cys Lys Tyr Asp Ser Phe Glu Ser Met Pro Pro

1460 1465 1470

Ala Glu Leu Ala Arg Val Glu Lys Arg Ile Phe Ser Arg Ala Leu Glu

1475 1480 1485

Glu Val Trp Asp Glu Thr Lys Asn Phe Tyr Ile Asn Arg Leu His Asn

1490 1495 1500

Pro Glu Lys Ile Gln Arg Ala Glu Arg Asp Pro Lys Leu Lys Met Ser

1505 1510 1515 1520

Leu Cys Phe Arg Trp Tyr Leu Ser Leu Ala Ser Arg Trp Ala Asn Thr

1525 1530 1535

Gly Ala Ser Asp Arg Val Met Asp Tyr Gln Val Trp Cys Gly Pro Ala

1540 1545 1550

Ile Gly Ser Phe Asn Asp Phe Ile Lys Gly Thr Tyr Leu Asp Pro Ala

1555 1560 1565

Val Ala Asn Glu Tyr Pro Cys Val Val Gln Ile Asn Lys Gln Ile Leu

1570 1575 1580

Arg Gly Ala Cys Phe Leu Arg Arg Leu Glu Ile Leu Arg Asn Ala Arg

1585 1590 1595 1600

Leu Ser Asp Gly Ala Ala Ala Leu Val Ala Ser Ile Asp Asp Thr Tyr

1605 1610 1615

Val Pro Ala Glu Lys Leu

1620

73

1551

Schizochytrium aggregatum

73

Arg Ala Glu Ala Gly Arg Glu Pro Glu Pro Ala Pro Gln Ile Thr Ser

1 5 10 15

Thr Ala Ala Glu Ser Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln

20 25 30

Gln Gln Gln Gln Pro Arg Glu Gly Asp Lys Glu Lys Ala Ala Glu Thr

35 40 45

Met Ala Leu Arg Val Lys Thr Asn Lys Lys Pro Cys Trp Glu Met Thr

50 55 60

Lys Glu Glu Leu Thr Ser Gly Lys Thr Glu Val Phe Asn Tyr Glu Glu

65 70 75 80

Leu Leu Glu Phe Ala Glu Gly Asp Ile Ala Lys Val Phe Gly Pro Glu

85 90 95

Phe Ala Val Ile Asp Lys Tyr Pro Arg Arg Val Arg Leu Pro Ala Arg

100 105 110

Glu Tyr Leu Leu Val Thr Arg Val Thr Leu Met Asp Ala Glu Val Asn

115 120 125

Asn Tyr Arg Val Gly Ala Arg Met Val Thr Glu Tyr Asp Leu Pro Val

130 135 140

Asn Gly Glu Leu Ser Glu Gly Gly Asp Cys Pro Trp Ala Val Leu Val

145 150 155 160

Glu Ser Gly Gln Cys Asp Leu Met Leu Ile Ser Tyr Met Gly Ile Asp

165 170 175

Phe Gln Asn Gln Gly Asp Arg Val Tyr Arg Leu Leu Asn Thr Thr Leu

180 185 190

Thr Phe Tyr Gly Val Ala His Glu Gly Glu Thr Leu Glu Tyr Asp Ile

195 200 205

Arg Val Thr Gly Phe Ala Lys Arg Leu Asp Gly Gly Ile Ser Met Phe

210 215 220

Phe Phe Glu Tyr Asp Cys Tyr Val Asn Gly Arg Leu Leu Ile Glu Met

225 230 235 240

Arg Asp Gly Cys Ala Gly Phe Phe Thr Asn Glu Glu Leu Asp Ala Gly

245 250 255

Lys Gly Val Val Phe Thr Arg Gly Asp Leu Ala Ala Arg Ala Lys Ile

260 265 270

Pro Lys Gln Asp Val Ser Pro Tyr Ala Val Ala Pro Cys Leu His Lys

275 280 285

Thr Lys Leu Asn Glu Lys Glu Met Gln Thr Leu Val Asp Lys Asp Trp

290 295 300

Ala Ser Val Phe Gly Ser Lys Asn Gly Met Pro Glu Ile Asn Tyr Lys

305 310 315 320

Leu Cys Ala Arg Lys Met Leu Met Ile Asp Arg Val Thr Ser Ile Asp

325 330 335

His Lys Gly Gly Val Tyr Gly Leu Gly Gln Leu Val Gly Glu Lys Ile

340 345 350

Leu Glu Arg Asp His Trp Tyr Phe Pro Cys His Phe Val Lys Asp Gln

355 360 365

Val Met Ala Gly Ser Leu Val Ser Asp Gly Cys Ser Gln Met Leu Lys

370 375 380

Met Tyr Met Ile Trp Leu Gly Leu His Leu Thr Thr Gly Pro Phe Asp

385 390 395 400

Phe Arg Pro Val Asn Gly His Pro Asn Lys Val Arg Cys Arg Gly Gln

405 410 415

Ile Ser Pro His Lys Gly Lys Leu Val Tyr Val Met Glu Ile Lys Glu

420 425 430

Met Gly Phe Asp Glu Asp Asn Asp Pro Tyr Ala Ile Ala Asp Val Asn

435 440 445

Ile Ile Asp Val Asp Phe Glu Lys Gly Gln Asp Phe Ser Leu Asp Arg

450 455 460

Ile Ser Asp Tyr Gly Lys Gly Asp Leu Asn Lys Lys Ile Val Val Asp

465 470 475 480

Phe Lys Gly Ile Ala Leu Lys Met Gln Lys Arg Ser Thr Asn Lys Asn

485 490 495

Pro Ser Lys Val Gln Pro Val Phe Ala Asn Gly Ala Ala Thr Val Gly

500 505 510

Pro Glu Ala Ser Lys Ala Ser Ser Gly Ala Ser Ala Ser Ala Ser Ala

515 520 525

Ala Pro Ala Lys Pro Ala Phe Ser Ala Asp Val Leu Ala Pro Lys Pro

530 535 540

Val Ala Leu Pro Glu His Ile Leu Lys Gly Asp Ala Leu Ala Pro Lys

545 550 555 560

Glu Met Ser Trp His Pro Met Ala Arg Ile Pro Gly Asn Pro Thr Pro

565 570 575

Ser Phe Ala Pro Ser Ala Tyr Lys Pro Arg Asn Ile Ala Phe Thr Pro

580 585 590

Phe Pro Gly Asn Pro Asn Asp Asn Asp His Thr Pro Gly Lys Met Pro

595 600 605

Leu Thr Trp Phe Asn Met Ala Glu Phe Met Ala Gly Lys Val Ser Met

610 615 620

Cys Leu Gly Pro Glu Phe Ala Lys Phe Asp Asp Ser Asn Thr Ser Arg

625 630 635 640

Ser Pro Ala Trp Asp Leu Ala Leu Val Thr Arg Ala Val Ser Val Ser

645 650 655

Asp Leu Lys His Val Asn Tyr Arg Asn Ile Asp Leu Asp Pro Ser Lys

660 665 670

Gly Thr Met Val Gly Glu Phe Asp Cys Pro Ala Asp Ala Trp Phe Tyr

675 680 685

Lys Gly Ala Cys Asn Asp Ala His Met Pro Tyr Ser Ile Leu Met Glu

690 695 700

Ile Ala Leu Gln Thr Ser Gly Val Leu Thr Ser Val Leu Lys Ala Pro

705 710 715 720

Leu Thr Met Glu Lys Asp Asp Ile Leu Phe Arg Asn Leu Asp Ala Asn

725 730 735

Ala Glu Phe Val Arg Ala Asp Leu Asp Tyr Arg Gly Lys Thr Ile Arg

740 745 750

Asn Val Thr Lys Cys Thr Gly Tyr Ser Met Leu Gly Glu Met Gly Val

755 760 765

His Arg Phe Thr Phe Glu Leu Tyr Val Asp Asp Val Leu Phe Tyr Lys

770 775 780

Gly Ser Thr Ser Phe Gly Trp Phe Val Pro Glu Val Phe Ala Ala Gln

785 790 795 800

Ala Gly Leu Asp Asn Gly Arg Lys Ser Glu Pro Trp Phe Ile Glu Asn

805 810 815

Lys Val Pro Ala Ser Gln Val Ser Ser Phe Asp Val Arg Pro Asn Gly

820 825 830

Ser Gly Arg Thr Ala Ile Phe Ala Asn Ala Pro Ser Gly Ala Gln Leu

835 840 845

Asn Arg Arg Thr Asp Gln Gly Gln Tyr Leu Asp Ala Val Asp Ile Val

850 855 860

Ser Gly Ser Gly Lys Lys Ser Leu Gly Tyr Ala His Gly Ser Lys Thr

865 870 875 880

Val Asn Pro Asn Asp Trp Phe Phe Ser Cys His Phe Trp Phe Asp Ser

885 890 895

Val Met Pro Gly Ser Leu Gly Val Glu Ser Met Phe Gln Leu Val Glu

900 905 910

Ala Ile Ala Ala His Glu Asp Leu Ala Gly Lys Ala Arg His Cys Gln

915 920 925

Pro His Leu Cys Ala Arg Pro Arg Ala Arg Ser Ser Trp Lys Tyr Arg

930 935 940

Gly Gln Leu Thr Pro Lys Ser Lys Lys Met Asp Ser Glu Val His Ile

945 950 955 960

Val Ser Val Asp Ala His Asp Gly Val Val Asp Leu Val Ala Asp Gly

965 970 975

Phe Leu Trp Ala Asp Ser Leu Arg Val Tyr Ser Val Ser Asn Ile Arg

980 985 990

Val Arg Ile Ala Ser Gly Glu Ala Pro Ala Ala Ala Ser Ser Ala Ala

995 1000 1005

Ser Val Gly Ser Ser Ala Ser Ser Val Glu Arg Thr Arg Ser Ser Pro

1010 1015 1020

Ala Val Ala Ser Gly Pro Ala Gln Thr Ile Asp Leu Lys Gln Leu Lys

1025 1030 1035 1040

Thr Glu Leu Leu Glu Leu Asp Ala Pro Leu Tyr Leu Ser Gln Asp Pro

1045 1050 1055

Thr Ser Gly Gln Leu Lys Lys His Thr Asp Val Ala Ser Gly Gln Ala

1060 1065 1070

Thr Ile Val Gln Pro Cys Thr Leu Gly Asp Leu Gly Asp Arg Ser Phe

1075 1080 1085

Met Glu Thr Tyr Gly Val Val Ala Pro Leu Tyr Thr Gly Ala Met Ala

1090 1095 1100

Lys Gly Ile Ala Ser Ala Asp Leu Val Ile Ala Ala Gly Lys Arg Lys

1105 1110 1115 1120

Ile Leu Gly Ser Phe Gly Ala Gly Gly Leu Pro Met His His Val Arg

1125 1130 1135

Ala Ala Leu Glu Lys Ile Gln Ala Ala Leu Pro Gln Gly Pro Tyr Ala

1140 1145 1150

Val Asn Leu Ile His Ser Pro Phe Asp Ser Asn Leu Glu Lys Gly Asn

1155 1160 1165

Val Asp Leu Phe Leu Glu Lys Gly Val Thr Val Val Glu Ala Ser Ala

1170 1175 1180

Phe Met Thr Leu Thr Pro Gln Val Val Arg Tyr Arg Ala Ala Gly Leu

1185 1190 1195 1200

Ser Arg Asn Ala Asp Gly Ser Val Asn Ile Arg Asn Arg Ile Ile Gly

1205 1210 1215

Lys Val Ser Arg Thr Glu Leu Ala Glu Met Phe Ile Arg Pro Ala Pro

1220 1225 1230

Glu His Leu Leu Glu Lys Leu Ile Ala Ser Gly Glu Ile Thr Gln Glu

1235 1240 1245

Gln Ala Glu Leu Ala Arg Arg Val Pro Val Ala Asp Asp Ile Ala Val

1250 1255 1260

Glu Ala Asp Ser Gly Gly His Thr Asp Asn Arg Pro Ile His Val Ile

1265 1270 1275 1280

Leu Pro Leu Ile Ile Asn Leu Arg Asn Arg Leu His Arg Glu Cys Gly

1285 1290 1295

Tyr Pro Ala His Leu Arg Val Arg Val Gly Ala Gly Gly Gly Val Gly

1300 1305 1310

Cys Pro Gln Ala Ala Ala Ala Ala Leu Thr Met Gly Ala Ala Phe Ile

1315 1320 1325

Val Thr Gly Thr Val Asn Gln Val Ala Lys Gln Ser Gly Thr Cys Asp

1330 1335 1340

Asn Val Arg Lys Gln Leu Ser Gln Ala Thr Tyr Ser Asp Ile Cys Met

1345 1350 1355 1360

Ala Pro Ala Ala Asp Met Phe Glu Glu Gly Val Lys Leu Gln Val Leu

1365 1370 1375

Lys Lys Gly Thr Met Phe Pro Ser Arg Ala Asn Lys Leu Tyr Glu Leu

1380 1385 1390

Phe Cys Lys Tyr Asp Ser Phe Asp Ser Met Pro Pro Ala Glu Leu Glu

1395 1400 1405

Arg Ile Glu Lys Arg Ile Phe Lys Arg Ala Leu Gln Glu Val Trp Glu

1410 1415 1420

Glu Thr Lys Asp Phe Tyr Ile Asn Gly Leu Lys Asn Pro Glu Lys Ile

1425 1430 1435 1440

Gln Arg Ala Glu His Asp Pro Lys Leu Lys Met Ser Leu Cys Phe Arg

1445 1450 1455

Trp Tyr Leu Gly Leu Ala Ser Arg Trp Ala Asn Met Gly Ala Pro Asp

1460 1465 1470

Arg Val Met Asp Tyr Gln Val Trp Cys Gly Pro Ala Ile Gly Ala Phe

1475 1480 1485

Asn Asp Phe Ile Lys Gly Thr Tyr Leu Asp Pro Ala Val Ser Asn Glu

1490 1495 1500

Tyr Pro Cys Val Val Gln Ile Asn Leu Gln Ile Leu Arg Gly Ala Cys

1505 1510 1515 1520

Tyr Leu Arg Arg Leu Asn Ala Leu Arg Asn Asp Pro Arg Ile Asp Leu

1525 1530 1535

Glu Thr Glu Asp Ala Ala Phe Val Tyr Glu Pro Thr Asn Ala Leu

1540 1545 1550

74

30

Schizochytrium aggregatum

74

taccgcggca agactatccg caacgtcacc 30

75

30

Schizochytrium aggregatum

75

gccgtcgtgg gcgtccacgg acacgatgtg 30

76

4767

Schizochytrium aggregatum

76

cgagcagagg ccggccgcga gcccgagccc gcgccgcaga tcactagtac cgctgcggaa 60

tcacagcagc agcagcagca gcagcagcag cagcagcagc agcagcagcc acgagaggga 120

gataaagaaa aagcggcaga gacgatggcg ctccgtgtca agacgaacaa gaagccatgc 180

tgggagatga ccaaggagga gctgaccagc ggcaagaccg aggtgttcaa ctatgaggaa 240

ctcctcgagt tcgcagaggg cgacatcgcc aaggtcttcg gacccgagtt cgccgtcatc 300

gacaagtacc cgcgccgcgt gcgcctgccc gcccgcgagt acctgctcgt gacccgcgtc 360

accctcatgg acgccgaggt caacaactac cgcgtcggcg cccgcatggt caccgagtac 420

gatctccccg tcaacggaga gctctccgag ggcggagact gcccctgggc cgtcctggtc 480

gagagtggcc agtgcgatct catgctcatc tcctacatgg gcattgactt ccagaaccag 540

ggcgaccgcg tctaccgcct gctcaacacc acgctcacct tttacggcgt ggcccacgag 600

ggcgagaccc tcgagtacga cattcgcgtc accggcttcg ccaagcgtct cgacggcggc 660

atctccatgt tcttcttcga gtacgactgc tacgtcaacg gccgcctcct catcgagatg 720

cgcgatggct gcgccggctt cttcaccaac gaggagctcg acgccggcaa gggcgtcgtc 780

ttcacccgcg gcgacctcgc cgcccgcgcc aagatcccaa agcaggacgt ctccccctac 840

gccgtcgccc cctgcctcca caagaccaag ctcaacgaaa aggagatgca gaccctcgtc 900

gacaaggact gggcatccgt ctttggctcc aagaacggca tgccggaaat caactacaaa 960

ctctgcgcgc gtaagatgct catgattgac cgcgtcacca gcattgacca caagggcggt 1020

gtctacggcc tcggtcagct cgtcggtgaa aagatcctcg agcgcgacca ctggtacttt 1080

ccctgccact ttgtcaagga tcaggtcatg gccggatccc tcgtctccga cggctgcagc 1140

cagatgctca agatgtacat gatctggctc ggcctccacc tcaccaccgg accctttgac 1200

ttccgcccgg tcaacggcca ccccaacaag gtccgctgcc gcggccaaat ctccccgcac 1260

aagggcaagc tcgtctacgt catggagatc aaggagatgg gcttcgacga ggacaacgac 1320

ccgtacgcca ttgccgacgt caacatcatt gatgtcgact tcgaaaaggg ccaggacttt 1380

agcctcgacc gcatcagcga ctacggcaag ggcgacctca acaagaagat cgtcgtcgac 1440

tttaagggca tcgctctcaa gatgcagaag cgctccacca acaagaaccc ctccaaggtt 1500

cagcccgtct ttgccaacgg cgccgccact gtcggccccg aggcctccaa ggcttcctcc 1560

ggcgccagcg ccagcgccag cgccgccccg gccaagcctg ccttcagcgc cgatgttctt 1620

gcgcccaagc ccgttgccct tcccgagcac atcctcaagg gcgacgccct cgcccccaag 1680

gagatgtcct ggcaccccat ggcccgcatc ccgggcaacc cgacgccctc ttttgcgccc 1740

tcggcctaca agccgcgcaa catcgccttt acgcccttcc ccggcaaccc caacgataac 1800

gaccacaccc cgggcaagat gccgctcacc tggttcaaca tggccgagtt catggccggc 1860

aaggtcagca tgtgcctcgg ccccgagttc gccaagttcg acgactcgaa caccagccgc 1920

agccccgctt gggacctcgc tctcgtcacc cgcgccgtgt ctgtgtctga cctcaagcac 1980

gtcaactacc gcaacatcga cctcgacccc tccaagggta ccatggtcgg cgagttcgac 2040

tgccccgcgg acgcctggtt ctacaagggc gcctgcaacg atgcccacat gccgtactcg 2100

atcctcatgg agatcgccct ccagacctcg ggtgtgctca cctcggtgct caaggcgccc 2160

ctgaccatgg agaaggacga catcctcttc cgcaacctcg acgccaacgc cgagttcgtg 2220

cgcgccgacc tcgactaccg cggcaagact atccgcaacg tcaccaagtg cactggctac 2280

agcatgctcg gcgagatggg cgtccaccgc ttcacctttg agctctacgt cgatgatgtg 2340

ctcttttaca agggctcgac ctcgttcggc tggttcgtgc ccgaggtytt tgccgcccag 2400

gccggcctcg acaacggccg caagtcggag ccctggttca ttgagaacaa ggttccggcc 2460

tcgcaggtct cctcctttga cgtgcgcccc aacggcagcg gccgcaccgc catcttcgcc 2520

aacgccccca gcggcgccca gctcaaccgc cgcacggacc agggccagta cctcgacgcc 2580

gtcgacattg tctccggcag cggcaagaag agcctcggct acgcccacgg ttccaagacg 2640

gtcaacccga acgactggtt cttctcgtgc cacttttggt ttgactcggt catgcccgga 2700

agtctcggtg tcgagtccat gttccagctc gtcgaggcca tcgccgccca cgaggatctc 2760

gctggcaaag cacggcattg ccaaccccac ctttgtgcac gcccccgggc aagatcaagc 2820

tggaagtacc gcggscagct cacgcccaag agcaagaaga tggactcgga ggtccacatc 2880

gtgtccgtgg acgcccacga cggcgttgtc gacctcgtcg ccgacggctt cctctgggcc 2940

gacagcctcc gcgtctactc ggtgagcaac attcgcgtgc gcatcgcctc cggtgaggcc 3000

cctgccgccg cctcctccgc cgcctctgtg ggctcctcgg cttcgtccgt cgagcgcacg 3060

cgctcgagcc ccgctgtcgc ctccggcccg gcccagacca tcgacctcaa gcagctcaag 3120

accgagctcc tcgagctcga tgccccgctc tacctctcgc aggacccgac cagcggccag 3180

ctcaagaagc acaccgacgt ggcctccggc caggccacca tcgtgcagcc ctgcacgctc 3240

ggcgacctcg gtgaccgctc cttcatggag acctacggcg tcgtcgcccc gctgtacacg 3300

ggcgccatgg ccaagggcat tgcctcggcg gacctcgtca tcgccgccgg caagcgcaag 3360

atcctcggct cctttggcgc cggcggcctc cccatgcacc acgtgcgcgc cgccctcgag 3420

aagatccagg ccgccctgcc tcagggcccc tacgccgtca acctcatcca ctcgcctttt 3480

gacagcaacc tcgagaaggg caacgtcgat ctcttcctcg agaagggcgt cactgtggtg 3540

gaggcctcgg cattcatgac cctcaccccg caggtcgtgc gctaccgcgc cgccggcctc 3600

tcgcgcaacg ccgacggttc ggtcaacatc cgcaaccgca tcatcggcaa ggtctcgcgc 3660

accgagctcg ccgagatgtt catccgcccg gccccggagc acctcctcga gaagctcatc 3720

gcctcgggcg agatcaccca ggagcaggcc gagctcgcgc gccgcgttcc cgtcgccgac 3780

gatatcgctg tcgaggctga ctcgggcggc cacaccgaca accgccccat ccacgtcatc 3840

ctcccgctca tcatcaacct ccgcaaccgc ctgcaccgcg agtgcggcta ccccgcgcac 3900

ctccgcgtcc gcgttggcgc cggcggtggc gtcggctgcc cgcaggccgc cgccgccgcg 3960

ctcaccatgg gcgccgcctt catcgtcacc ggcactgtca accaggtcgc caagcagtcc 4020

ggcacctgcg acaacgtgcg caagcagctc tcgcaggcca cctactcgga tatctgcatg 4080

gccccggccg ccgacatgtt cgaggagggc gtcaagctcc aggtcctcaa gaagggaacc 4140

atgttcccct cgcgcgccaa caagctctac gagctctttt gcaagtacga ctccttcgac 4200

tccatgcctc ctgccgagct cgagcgcatc gagaagcgta tcttcaagcg cgcactccag 4260

gaggtctggg aggagaccaa ggacttttac attaacggtc tcaagaaccc ggagaagatc 4320

cagcgcgccg agcacgaccc caagctcaag atgtcgctct gcttccgctg gtaccttggt 4380

cttgccagcc gctgggccaa catgggcgcc ccggaccgcg tcatggacta ccaggtctgg 4440

tgtggcccgg ccattggcgc cttcaacgac ttcatcaagg gcacctacct cgaccccgct 4500

gtctccaacg agtacccctg tgtcgtccag atcaacctgc aaatcctccg tggtgcctgc 4560

tacctgcgcc gtctcaacgc cctgcgcaac gacccgcgca ttgacctcga gaccgaggat 4620

gctgcctttg tctacgagcc caccaacgcg ctctaagaaa gtgaaccttg tcctaacccg 4680

acagcgaatg gcgggagggg gcgggctaaa agatcgtatt acatagtatt tttcccctac 4740

tctttgtgaa aaaaaaaaaa aaaaaaa 4767

77

7959

Vibrio marinus

77

atggctaaaa agaacaccac atcgattaag cacgccaagg atgtgttaag tagtgatgat 60

caacagttaa attctcgctt gcaagaatgt ccgattgcca tcattggtat ggcatcggtt 120

tttgcagatg ctaaaaactt ggatcaattc tgggataaca tcgttgactc tgtggacgct 180

attattgatg tgcctagcga tcgctggaac attgacgacc attactcggc tgataaaaaa 240

gcagctgaca agacatactg caaacgcggt ggtttcattc cagagcttga ttttgatccg 300

atggagtttg gtttaccgcc aaatatcctc gagttaactg acatcgctca attgttgtca 360

ttaattgttg ctcgtgatgt attaagtgat gctggcattg gtagtgatta tgaccatgat 420

aaaattggta tcacgctggg tgtcggtggt ggtcagaaac aaatttcgcc attaacgtcg 480

cgcctacaag gcccggtatt agaaaaagta ttaaaagcct caggcattga tgaagatgat 540

cgcgctatga tcatcgacaa atttaaaaaa gcctacatcg gctgggaaga gaactcattc 600

ccaggcatgc taggtaacgt tattgctggt cgtatcgcca atcgttttga ttttggtggt 660

actaactgtg tggttgatgc ggcatgcgct ggctcccttg cagctgttaa aatggcgatc 720

tcagacttac ttgaatatcg ttcagaagtc atgatatcgg gtggtgtatg ttgtgataac 780

tcgccattca tgtatatgtc attctcgaaa acaccagcat ttaccaccaa tgatgatatc 840

cgtccgtttg atgacgattc aaaaggcatg ctggttggtg aaggtattgg catgatggcg 900

tttaaacgtc ttgaagatgc tgaacgtgac ggcgacaaaa tttattctgt actgaaaggt 960

atcggtacat cttcagatgg tcgtttcaaa tctatttacg ctccacgccc agatggccaa 1020

gcaaaagcgc taaaacgtgc ttatgaagat gccggttttg cccctgaaac atgtggtcta 1080

attgaaggcc atggtacggg taccaaagcg ggtgatgccg cagaatttgc tggcttgacc 1140

aaacactttg gcgccgccag tgatgaaaag caatatatcg ccttaggctc agttaaatcg 1200

caaattggtc atactaaatc tgcggctggc tctgcgggta tgattaaggc ggcattagcg 1260

ctgcatcata aaatcttacc tgcaacgatc catatcgata aaccaagtga agccttggat 1320

atcaaaaaca gcccgttata cctaaacagc gaaacgcgtc cttggatgcc acgtgaagat 1380

ggtattccac gtcgtgcagg tatcagctca tttggttttg gcggcaccaa cttccatatt 1440

attttagaag agtatcgccc aggtcacgat agcgcatatc gcttaaactc agtgagccaa 1500

actgtgttga tctcggcaaa cgaccaacaa ggtattgttg ctgagttaaa taactggcgt 1560

actaaactgg ctgtcgatgc tgatcatcaa gggtttgtat ttaatgagtt agtgacaacg 1620

tggccattaa aaaccccatc cgttaaccaa gctcgtttag gttttgttgc gcgtaatgca 1680

aatgaagcga tcgcgatgat tgatacggca ttgaaacaat tcaatgcgaa cgcagataaa 1740

atgacatggt cagtacctac cggggtttac tatcgtcaag ccggtattga tgcaacaggt 1800

aaagtggttg cgctattctc agggcaaggt tcgcaatacg tgaacatggg tcgtgaatta 1860

acctgtaact tcccaagcat gatgcacagt gctgcggcga tggataaaga gttcagtgcc 1920

gctggtttag gccagttatc tgcagttact ttccctatcc ctgtttatac ggatgccgag 1980

cgtaagctac aagaagagca attacgttta acgcaacatg cgcaaccagc gattggtagt 2040

ttgagtgttg gtctgttcaa aacgtttaag caagcaggtt ttaaagctga ttttgctgcc 2100

ggtcatagtt tcggtgagtt aaccgcatta tgggctgccg atgtattgag cgaaagcgat 2160

tacatgatgt tagcgcgtag tcgtggtcaa gcaatggctg cgccagagca acaagatttt 2220

gatgcaggta agatggccgc tgttgttggt gatccaaagc aagtcgctgt gatcattgat 2280

acccttgatg atgtctctat tgctaacttc aactcgaata accaagttgt tattgctggt 2340

actacggagc aggttgctgt agcggttaca accttaggta atgctggttt caaagttgtg 2400

ccactgccgg tatctgctgc gttccataca cctttagttc gtcacgcgca aaaaccattt 2460

gctaaagcgg ttgatagcgc taaatttaaa gcgccaagca ttccagtgtt tgctaatggc 2520

acaggcttgg tgcattcaag caaaccgaat gacattaaga aaaacctgaa aaaccacatg 2580

ctggaatctg ttcatttcaa tcaagaaatt gacaacatct atgctgatgg tggccgcgta 2640

tttatcgaat ttggtccaaa gaatgtatta actaaattgg ttgaaaacat tctcactgaa 2700

aaatctgatg tgactgctat cgcggttaat gctaatccta aacaacctgc ggacgtacaa 2760

atgcgccaag ctgcgctgca aatggcagtg cttggtgtcg cattagacaa tattgacccg 2820

tacgacgccg ttaagcgtcc acttgttgcg ccgaaagcat caccaatgtt gatgaagtta 2880

tctgcagcgt cttatgttag tccgaaaacg aagaaagcgt ttgctgatgc attgactgat 2940

ggctggactg ttaagcaagc gaaagctgta cctgctgttg tgtcacaacc acaagtgatt 3000

gaaaagatcg ttgaagttga aaagatagtt gaacgcattg tcgaagtaga gcgtattgtc 3060

gaagtagaaa aaatcgtcta cgttaatgct gacggttcgc ttatatcgca aaataatcaa 3120

gacgttaaca gcgctgttgt tagcaacgtg actaatagct cagtgactca tagcagtgat 3180

gctgaccttg ttgcctctat tgaacgcagt gttggtcaat ttgttgcaca ccaacagcaa 3240

ttattaaatg tacatgaaca gtttatgcaa ggtccacaag actacgcgaa aacagtgcag 3300

aacgtacttg ctgcgcagac gagcaatgaa ttaccggaaa gtttagaccg tacattgtct 3360

atgtataacg agttccaatc agaaacgcta cgtgtacatg aaacgtacct gaacaatcag 3420

acgagcaaca tgaacaccat gcttactggt gctgaagctg atgtgctagc aaccccaata 3480

actcaggtag tgaatacagc cgttgccact agtcacaagg tagttgctcc agttattgct 3540

aatacagtga cgaatgttgt atctagtgtc agtaataacg cggcggttgc agtgcaaact 3600

gtggcattag cgcctacgca agaaatcgct ccaacagtcg ctactacgcc agcacccgca 3660

ttggttgcta tcgtggctga acctgtgatt gttgcgcatg ttgctacaga agttgcacca 3720

attacaccat cagttacacc agttgtcgca actcaagcgg ctatcgatgt agcaactatt 3780

aacaaagtaa tgttagaagt tgttgctgat aaaaccggtt atccaacgga tatgctggaa 3840

ctgagcatgg acatggaagc tgacttaggt atcgactcaa tcaaacgtgt tgagatatta 3900

ggcgcagtac aggaattgat ccctgactta cctgaactta atcctgaaga tcttgctgag 3960

ctacgcacgc ttggtgagat tgtcgattac atgaattcaa aagcccaggc tgtagctcct 4020

acaacagtac ctgtaacaag tgcacctgtt tcgcctgcat ctgctggtat tgatttagcc 4080

cacatccaaa acgtaatgtt agaagtggtt gcagacaaaa ccggttaccc aacagacatg 4140

ctagaactga gcatggatat ggaagctgac ttaggtattg attcaatcaa gcgtgtggaa 4200

atcttaggtg cagtacagga gatcataact gatttacctg agctaaaccc tgaagatctt 4260

gctgaattac gcaccctagg tgaaatcgtt agttacatgc aaagcaaagc gccagtcgct 4320

gaaagtgcgc cagtggcgac ggctcctgta gcaacaagct cagcaccgtc tatcgatttg 4380

aaccacattc aaacagtgat gatggatgta gttgcagata agactggtta tccaactgac 4440

atgctagaac ttggcatgga catggaagct gatttaggta tcgattcaat caaacgtgtg 4500

gaaatattag gcgcagtgca ggagatcatc actgatttac ctgagctaaa cccagaagac 4560

ctcgctgaat tacgcacgct aggtgaaatc gttagttaca tgcaaagcaa agcgccagtc 4620

gctgagagtg cgccagtagc gacggcttct gtagcaacaa gctctgcacc gtctatcgat 4680

ttaaaccata tccaaacagt gatgatggaa gtggttgcag acaaaaccgg ttatccagta 4740

gacatgttag aacttgctat ggacatggaa gctgacctag gtatcgattc aatcaagcgt 4800

gtagaaattt taggtgcggt acaggaaatc attactgact tacctgagct taaccctgaa 4860

gatcttgctg aactacgtac attaggtgaa atcgttagtt acatgcaaag caaagcgccc 4920

gtagctgaag cgcctgcagt acctgttgca gtagaaagtg cacctactag tgtaacaagc 4980

tcagcaccgt ctatcgattt agaccacatc caaaatgtaa tgatggatgt tgttgctgat 5040

aagactggtt atcctgccaa tatgcttgaa ttagcaatgg acatggaagc cgaccttggt 5100

attgattcaa tcaagcgtgt tgaaattcta ggcgcggtac aggagatcat tactgattta 5160

cctgaactaa acccagaaga cttagctgaa ctacgtacgt tagaagaaat tgtaacctac 5220

atgcaaagca aggcgagtgg tgttactgta aatgtagtgg ctagccctga aaataatgct 5280

gtatcagatg catttatgca aagcaatgtg gcgactatca cagcggccgc agaacataag 5340

gcggaattta aaccggcgcc gagcgcaacc gttgctatct ctcgtctaag ctctatcagt 5400

aaaataagcc aagattgtaa aggtgctaac gccttaatcg tagctgatgg cactgataat 5460

gctgtgttac ttgcagacca cctattgcaa actggctgga atgtaactgc attgcaacca 5520

acttgggtag ctgtaacaac gacgaaagca tttaataagt cagtgaacct ggtgacttta 5580

aatggcgttg atgaaactga aatcaacaac attattactg ctaacgcaca attggatgca 5640

gttatctatc tgcacgcaag tagcgaaatt aatgctatcg aatacccaca agcatctaag 5700

caaggcctga tgttagcctt cttattagcg aaattgagta aagtaactca agccgctaaa 5760

gtgcgtggcg cctttatgat tgttactcag cagggtggtt cattaggttt tgatgatatc 5820

gattctgcta caagtcatga tgtgaaaaca gacctagtac aaagcggctt aaacggttta 5880

gttaagacac tgtctcacga gtgggataac gtattctgtc gtgcggttga tattgcttcg 5940

tcattaacgg ctgaacaagt tgcaagcctt gttagtgatg aactacttga tgctaacact 6000

gtattaacag aagtgggtta tcaacaagct ggtaaaggcc ttgaacgtat cacgttaact 6060

ggtgtggcta ctgacagcta tgcattaaca gctggcaata acatcgatgc taactcggta 6120

tttttagtga gtggtggcgc aaaaggtgta actgcacatt gtgttgctcg tatagctaaa 6180

gaatatcagt ctaagttcat cttattggga cgttcaacgt tctcaagtga cgaaccgagc 6240

tgggcaagtg gtattactga tgaagcggcg ttaaagaaag cagcgatgca gtctttgatt 6300

acagcaggtg ataaaccaac acccgttaag atcgtacagc taatcaaacc aatccaagct 6360

aatcgtgaaa ttgcgcaaac cttgtctgca attaccgctg ctggtggcca agctgaatat 6420

gtttctgcag atgtaactaa tgcagcaagc gtacaaatgg cagtcgctcc agctatcgct 6480

aagttcggtg caatcactgg catcattcat ggcgcgggtg tgttagctga ccaattcatt 6540

gagcaaaaaa cactgagtga ttttgagtct gtttacagca ctaaaattga cggtttgtta 6600

tcgctactat cagtcactga agcaagcaac atcaagcaat tggtattgtt ctcgtcagcg 6660

gctggtttct acggtaaccc cggccagtct gattactcga ttgccaatga gatcttaaat 6720

aaaaccgcat accgctttaa atcattgcac ccacaagctc aagtattgag ctttaactgg 6780

ggtccttggg acggtggcat ggtaacgcct gagcttaaac gtatgtttga ccaacgtggt 6840

gtttacatta ttccacttga tgcaggtgca cagttattgc tgaatgaact agccgctaat 6900

gataaccgtt gtccacaaat cctcgtgggt aatgacttat ctaaagatgc tagctctgat 6960

caaaagtctg atgaaaagag tactgctgta aaaaagccac aagttagtcg tttatcagat 7020

gctttagtaa ctaaaagtat caaagcgact aacagtagct ctttatcaaa caagactagt 7080

gctttatcag acagtagtgc ttttcaggtt aacgaaaacc actttttagc tgaccacatg 7140

atcaaaggca atcaggtatt accaacggta tgcgcgattg cttggatgag tgatgcagca 7200

aaagcgactt atagtaaccg agactgtgca ttgaagtatg tcggtttcga agactataaa 7260

ttgtttaaag gtgtggtttt tgatggcaat gaggcggcgg attaccaaat ccaattgtcg 7320

cctgtgacaa gggcgtcaga acaggattct gaagtccgta ttgccgcaaa gatctttagc 7380

ctgaaaagtg acggtaaacc tgtgtttcat tatgcagcga caatattgtt agcaactcag 7440

ccacttaatg ctgtgaaggt agaacttccg acattgacag aaagtgttga tagcaacaat 7500

aaagtaactg atgaagcaca agcgttatac agcaatggca ccttgttcca cggtgaaagt 7560

ctgcagggca ttaagcagat attaagttgt gacgacaagg gcctgctatt ggcttgtcag 7620

ataaccgatg ttgcaacagc taagcaggga tccttcccgt tagctgacaa caatatcttt 7680

gccaatgatt tggtttatca ggctatgttg gtctgggtgc gcaaacaatt tggtttaggt 7740

agcttacctt cggtgacaac ggcttggact gtgtatcgtg aagtggttgt agatgaagta 7800

ttttatctgc aacttaatgt tgttgagcat gatctattgg gttcacgcgg cagtaaagcc 7860

cgttgtgata ttcaattgat tgctgctgat atgcaattac ttgccgaagt gaaatcagcg 7920

caagtcagtg tcagtgacat tttgaacgat atgtcatga 7959

78

2652

Vibrio marinus

78

atgacggaat tagctgttat tggtatggat gctaaattta gcggacaaga caatattgac 60

cgtgtggaac gcgctttcta tgaaggtgct tatgtaggta atgttagccg cgttagtacc 120

gaatctaatg ttattagcaa tggcgaagaa caagttatta ctgccatgac agttcttaac 180

tctgtcagtc tactagcgca aacgaatcag ttaaatatag ctgatatcgc ggtgttgctg 240

attgctgatg taaaaagtgc tgatgatcag cttgtagtcc aaattgcatc agcaattgaa 300

aaacagtgtg cgagttgtgt tgttattgct gatttaggcc aagcattaaa tcaagtagct 360

gatttagtta ataaccaaga ctgtcctgtg gctgtaattg gcatgaataa ctcggttaat 420

ttatctcgtc atgatcttga atctgtaact gcaacaatca gctttgatga aaccttcaat 480

ggttataaca atgtagctgg gttcgcgagt ttacttatcg cttcaactgc gtttgccaat 540

gctaagcaat gttatatata cgccaacatt aagggcttcg ctcaatcggg cgtaaatgct 600

caatttaacg ttggaaacat tagcgatact gcaaagaccg cattgcagca agctagcata 660

actgcagagc aggttggttt gttagaagtg tcagcagtcg ctgattcggc aatcgcattg 720

tctgaaagcc aaggtttaat gtctgcttat catcatacgc aaactttgca tactgcatta 780

agcagtgccc gtagtgtgac tggtgaaggc gggtgttttt cacaggtcgc aggtttattg 840

aaatgtgtaa ttggtttaca tcaacgttat attccggcga ttaaagattg gcaacaaccg 900

agtgacaatc aaatgtcacg gtggcggaat tcaccattct atatgcctgt agatgctcga 960

ccttggttcc cacatgctga tggctctgca cacattgccg cttatagttg tgtgactgct 1020

gacagctatt gtcatattct tttacaagaa aacgtcttac aagaacttgt tttgaaagaa 1080

acagtcttgc aagataatga cttaactgaa agcaagcttc agactcttga acaaaacaat 1140

ccagtagctg atctgcgcac taatggttac tttgcatcga gcgagttagc attaatcata 1200

gtacaaggta atgacgaagc acaattacgc tgtgaattag aaactattac agggcagtta 1260

agtactactg gcataagtac tatcagtatt aaacagatcg cagcagactg ttatgcccgt 1320

aatgatacta acaaagccta tagcgcagtg cttattgccg agactgctga agagttaagc 1380

aaagaaataa ccttggcgtt tgctggtatc gctagcgtgt ttaatgaaga tgctaaagaa 1440

tggaaaaccc cgaagggcag ttattttacc gcgcagcctg caaataaaca ggctgctaac 1500

agcacacaga atggtgtcac cttcatgtac ccaggtattg gtgctacata tgttggttta 1560

gggcgtgatc tatttcatct attcccacag atttatcagc ctgtagcggc tttagccgat 1620

gacattggcg aaagtctaaa agatacttta cttaatccac gcagtattag tcgtcatagc 1680

tttaaagaac tcaagcagtt ggatctggac ctgcgcggta acttagccaa tatcgctgaa 1740

gccggtgtgg gttttgcttg tgtgtttacc aaggtatttg aagaagtctt tgccgttaaa 1800

gctgactttg ctacaggtta tagcatgggt gaagtaagca tgtatgcagc actaggctgc 1860

tggcagcaac cgggattgat gagtgctcgc cttgcacaat cgaatacctt taatcatcaa 1920

ctttgcggcg agttaagaac actacgtcag cattggggca tggatgatgt agctaacggt 1980

acgttcgagc agatctggga aacctatacc attaaggcaa cgattgaaca ggtcgaaatt 2040

gcctctgcag atgaagatcg tgtgtattgc accattatca atacacctga tagcttgttg 2100

ttagccggtt atccagaagc ctgtcagcga gtcattaaga atttaggtgt gcgtgcaatg 2160

gcattgaata tggcgaacgc aattcacagc gcgccagctt atgccgaata cgatcatatg 2220

gttgagctat accatatgga tgttactcca cgtattaata ccaagatgta ttcaagctca 2280

tgttatttac cgattccaca acgcagcaaa gcgatttccc acagtattgc taaatgtttg 2340

tgtgatgtgg tggatttccc acgtttggtt aataccttac atgacaaagg tgcgcgggta 2400

ttcattgaaa tgggtccagg tcgttcgtta tgtagctggg tagataagat cttagttaat 2460

ggcgatggcg ataataaaaa gcaaagccaa catgtatctg ttcctgtgaa tgccaaaggc 2520

accagtgatg aacttactta tattcgtgcg attgctaagt taattagtca tggcgtgaat 2580

ttgaatttag atagcttgtt taacgggtca atcctggtta aagcaggcca tatagcaaac 2640

acgaacaaat ag 2652

79

6057

Vibrio marinus

79

atggatttaa agagagtaat tatggaaaat attgcagtag taggtattgc taatttgttc 60

ccgggctcac aagcaccgga tcaattttgg cagcaattgc ttgaacaaca agattgccgc 120

agtaaggcga ccgctgttca aatgggcgtt gatcctgcta aatataccgc caacaaaggt 180

gacacagata aattttactg tgtgcacggc ggttacatca gtgatttcaa ttttgatgct 240

tcaggttatc aactcgataa tgattattta gccggtttag atgaccttaa tcaatggggg 300

ctttatgtta cgaaacaagc ccttaccgat gcgggttatt ggggcagtac tgcactagaa 360

aactgtggtg tgattttagg taatttgtca ttcccaacta aatcatctaa tcagctgttt 420

atgcctttgt atcatcaagt tgttgataat gccttaaagg cggtattaca tcctgatttt 480

caattaacgc attacacagc accgaaaaaa acacatgctg acaatgcatt agtagcaggt 540

tatccagctg cattgatcgc gcaagcggcg ggtcttggtg gttcacattt tgcactggat 600

gcggcttgtg cttcatcttg ttatagcgtt aagttagcgt gtgattacct gcatacgggt 660

aaagccaaca tgatgcttgc tggtgcggta tctgcagcag atcctatgtt cgtaaatatg 720

ggtttctcga tattccaagc ttacccagct aacaatgtac atgccccgtt tgaccaaaat 780

tcacaaggtc tatttgccgg tgaaggcgcg ggcatgatgg tattgaaacg tcaaagtgat 840

gcagtacgtg atggtgatca tatttacgcc attattaaag gcggcgcatt atcgaatgac 900

ggtaaaggcg agtttgtatt aagcccgaac accaagggcc aagtattagt atatgaacgt 960

gcttatgccg atgcagatgt tgacccgagt acagttgact atattgaatg tcatgcaacg 1020

ggcacaccta agggtgacaa tgttgaattg cgttcgatgg aaaccttttt cagtcgcgta 1080

aataacaaac cattactggg ctcggttaaa tctaaccttg gtcatttgtt aactgccgct 1140

ggtatgcctg gcatgaccaa agctatgtta gcgctaggta aaggtcttat tcctgcaacg 1200

attaacttaa agcaaccact gcaatctaaa aacggttact ttactggcga gcaaatgcca 1260

acgacgactg tgtcttggcc aacaactccg ggtgccaagg cagataaacc gcgtaccgca 1320

ggtgtgagcg tatttggttt tggtggcagc aacgcccatt tggtattaca acagccaacg 1380

caaacactcg agactaattt tagtgttgct aaaccacgtg agcctttggc tattattggt 1440

atggacagcc attttggtag tgccagtaat ttagcgcagt tcaaaacctt attaaataat 1500

aatcaaaata ccttccgtga attaccagaa caacgctgga aaggcatgga aagtaacgct 1560

aacgtcatgc agtcgttaca attacgcaaa gcgcctaaag gcagttacgt tgaacagcta 1620

gatattgatt tcttgcgttt taaagtaccg cctaatgaaa aagattgctt gatcccgcaa 1680

cagttaatga tgatgcaagt ggcagacaat gctgcgaaag acggaggtct agttgaaggt 1740

cgtaatgttg cggtattagt agcgatgggc atggaactgg aattacatca gtatcgtggt 1800

cgcgttaatc taaccaccca aattgaagac agcttattac agcaaggtat taacctgact 1860

gttgagcaac gtgaagaact gaccaatatt gctaaagacg gtgttgcctc ggctgcacag 1920

ctaaatcagt atacgagttt cattggtaat attatggcgt cacgtatttc ggcgttatgg 1980

gatttttctg gtcctgctat taccgtatcg gctgaagaaa actctgttta tcgttgtgtt 2040

gaattagctg aaaatctatt tcaaaccagt gatgttgaag ccgttattat tgctgctgtt 2100

gatttgtctg gttcaattga aaacattact ttacgtcagc actacggtcc agttaatgaa 2160

aagggatctg taagtgaatg tggtccggtt aatgaaagca gttcagtaac caacaatatt 2220

cttgatcagc aacaatggct ggtgggtgaa ggcgcagcgg ctattgtcgt taaaccgtca 2280

tcgcaagtca ctgctgagca agtttatgcg cgtattgatg cggtgagttt tgcccctggt 2340

agcaatgcga aagcaattac gattgcagcg gataaagcat taacacttgc tggtatcagt 2400

gctgctgatg tagctagtgt tgaagcacat gcaagtggtt ttagtgccga aaataatgct 2460

gaaaaaaccg cgttaccgac tttataccca agcgcaagta tcagttcggt gaaagccaat 2520

attggtcata cgtttaatgc ctcgggtatg gcgagtatta ttaaaacggc gctgctgtta 2580

gatcagaata cgagtcaaga tcagaaaagc aaacatattg ctattaacgg tctaggtcgt 2640

gataacagct gcgcgcatct tatcttatcg agttcagcgc aagcgcatca agttgcacca 2700

gcgcctgtat ctggtatggc caagcaacgc ccacagttag ttaaaaccat caaactcggt 2760

ggtcagttaa ttagcaacgc gattgttaac agtgcgagtt catctttaca cgctattaaa 2820

gcgcagtttg ccggtaagca cttaaacaaa gttaaccagc cagtgatgat ggataacctg 2880

aagccccaag gtattagcgc tcatgcaacc aatgagtatg tggtgactgg agctgctaac 2940

actcaagctt ctaacattca agcatctcat gttcaagcgt caagtcatgc acaagagata 3000

gcaccaaacc aagttcaaaa tatgcaagct acagcagccg ctgtaagttc acccctttct 3060

caacatcaac acacagcgca gcccgtagcg gcaccgagcg ttgttggagt gactgtgaaa 3120

cataaagcaa gtaaccaaat tcatcagcaa gcgtctacgc ataaagcatt tttagaaagt 3180

cgtttagctg cacagaaaaa cctatcgcaa cttgttgaat tgcaaaccaa gctgtcaatc 3240

caaactggta gtgacaatac atctaacaat actgcgtcaa caagcaatac agtgctaaca 3300

aatcctgtat cagcaacgcc attaacactt gtgtctaatg cgcctgtagt agcgacaaac 3360

ctaaccagta cagaagcaaa agcgcaagca gctgctacac aagctggttt tcagataaaa 3420

ggacctgttg gttacaacta tccaccgctg cagttaattg aacgttataa taaaccagaa 3480

aacgtgattt acgatcaagc tgatttggtt gaattcgctg aaggtgatat tggtaaggta 3540

tttggtgctg aatacaatat tattgatggc tattcgcgtc gtgtacgtct gccaacctca 3600

gattacttgt tagtaacacg tgttactgaa cttgatgcca aggtgcatga atacaagaaa 3660

tcatacatgt gtactgaata tgatgtgcct gttgatgcac cgttcttaat tgatggtcag 3720

atcccttggt ctgttgccgt cgaatcaggc cagtgtgatt tgatgttgat ttcatatatc 3780

ggtattgatt tccaagcgaa aggcgaacgt gtttaccgtt tacttgattg tgaattaact 3840

ttccttgaag agatggcttt tggtggcgat actttacgtt acgagatcca cattgattcg 3900

tatgcacgta acggcgagca attattattc ttcttccatt acgattgtta cgtaggggat 3960

aagaaggtac ttatcatgcg taatggttgt gctggtttct ttactgacga agaactttct 4020

gatggtaaag gcgttattca taacgacaaa gacaaagctg agtttagcaa tgctgttaaa 4080

tcatcattca cgccgttatt acaacataac cgtggtcaat acgattataa cgacatgatg 4140

aagttggtta atggtgatgt tgccagttgt tttggtccgc aatatgatca aggtggccgt 4200

aatccatcat tgaaattctc gtctgagaag ttcttgatga ttgaacgtat taccaagata 4260

gacccaaccg gtggtcattg gggactaggc ctgttagaag gtcagaaaga tttagaccct 4320

gagcattggt atttcccttg tcactttaaa ggtgatcaag taatggctgg ttcgttgatg 4380

tcggaaggtt gtggccaaat ggcgatgttc ttcatgctgt ctcttggtat gcataccaat 4440

gtgaacaacg ctcgtttcca accactacca ggtgaatcac aaacggtacg ttgtcgtggg 4500

caagtactgc cacagcgcaa taccttaact taccgtatgg aagttactgc gatgggtatg 4560

catccacagc cattcatgaa agctaatatt gatattttgc ttgacggtaa agtggttgtt 4620

gatttcaaaa acttgagcgt gatgatcagc gaacaagatg agcattcaga ttaccctgta 4680

acactgccga gtaatgtggc gcttaaagcg attactgcac ctgttgcgtc agtagcacca 4740

gcatcttcac ccgctaacag cgcggatcta gacgaacgtg gtgttgaacc gtttaagttt 4800

cctgaacgtc cgttaatgcg tgttgagtca gacttgtctg caccgaaaag caaaggtgtg 4860

acaccgatta agcattttga agcgcctgct gttgctggtc atcatagagt gcctaaccaa 4920

gcaccgttta caccttggca tatgtttgag tttgcgacgg gtaatatttc taactgtttc 4980

ggtcctgatt ttgatgttta tgaaggtcgt attccacctc gtacaccttg tggcgattta 5040

caagttgtta ctcaggttgt agaagtgcag ggcgaacgtc ttgatcttaa aaatccatca 5100

agctgtgtag ctgaatacta tgtaccggaa gacgcttggt actttactaa aaacagccat 5160

gaaaactgga tgccttattc attaatcatg gaaattgcat tgcaaccaaa tggctttatt 5220

tctggttaca tgggcacgac gcttaaatac cctgaaaaag atctgttctt ccgtaacctt 5280

gatggtagcg gcacgttatt aaagcagatt gatttacgcg gcaagaccat tgtgaataaa 5340

tcagtcttgg ttagtacggc tattgctggt ggcgcgatta ttcaaagttt cacgtttgat 5400

atgtctgtag atggcgagct attttatact ggtaaagctg tatttggtta ctttagtggt 5460

gaatcactga ctaaccaact gggcattgat aacggtaaaa cgactaatgc gtggtttgtt 5520

gataacaata cccccgcagc gaatattgat gtgtttgatt taactaatca gtcattggct 5580

ctgtataaag cgcctgtgga taaaccgcat tataaattgg ctggtggtca gatgaacttt 5640

atcgatacag tgtcagtggt tgaaggcggt ggtaaagcgg gcgtggctta tgtttatggc 5700

gaacgtacga ttgatgctga tgattggttc ttccgttatc acttccacca agatccggtg 5760

atgccaggtt cattaggtgt tgaagctatt attgagttga tgcagaccta tgcgcttaaa 5820

aatgatttgg gtggcaagtt tgctaaccca cgtttcattg cgccgatgac gcaagttgat 5880

tggaaatacc gtgggcaaat tacgccgctg aataaacaga tgtcactgga cgtgcatatc 5940

actgagatcg tgaatgacgc tggtgaagtg cgaatcgttg gtgatgcgaa tctgtctaaa 6000

gatggtctgc gtatttatga agttaaaaac atcgttttaa gtattgttga agcgtaa 6057

80

1665

Vibrio marinus

80

atgaatatag taagtaatca ttcggcagct acaaaaaagg aattaagaat gtcgagttta 60

ggttttaaca ataacaacgc aattaactgg gcttggaaag tagatccagc gtcagttcat 120

acacaagatg cagaaattaa agcagcttta atggatctaa ctaaacctct ctatgtggcg 180

aataattcag gcgtaactgg tatagctaat catacgtcag tagcaggtgc gatcagcaat 240

aacatcgatg ttgatgtatt ggcgtttgcg caaaagttaa acccagaaga tctgggtgat 300

gatgcttaca agaaacagca cggcgttaaa tatgcttatc atggcggtgc gatggcaaat 360

ggtattgcct cggttgaatt ggttgttgcg ttaggtaaag cagggctgtt atgttcattt 420

ggtgctgcag gtctagtgcc tgatgcggtt gaagatgcaa ttcgtcgtat tcaagctgaa 480

ttaccaaatg gcccttatgc ggttaacttg atccatgcac cagcagaaga agcattagag 540

cgtggcgcgg ttgaacgttt cctaaaactt ggcgtcaaga cggtagaggc ttcagcttac 600

cttggtttaa ctgaacacat tgtttggtat cgtgctgctg gtctaactaa aaacgcagat 660

ggcagtgtta atatcggtaa caaggttatc gctaaagtat cgcgtaccga agttggtcgc 720

cgctttatgg aacctgcacc gcaaaaatta ctggataagt tattagaaca aaataagatc 780

acccctgaac aagctgcttt agcgttgctt gtacctatgg ctgatgatat tactggggaa 840

gcggattctg gtggtcatac agataaccgt ccgtttttaa cattattacc gacgattatt 900

ggtctgcgtg atgaagtgca agcgaagtat aacttctctc ctgcattacg tgttggtgct 960

ggtggtggta tcggaacgcc tgaagcagca ctcgctgcat ttaacatggg cgcggcttat 1020

atcgttctgg gttctgtgaa tcaggcgtgt gttgaagcgg gtgcatctga atatactcgt 1080

aaactgttat cgacagttga aatggctgat gtgactatgg cacctgctgc agatatgttt 1140

gaaatgggtg tgaagctgca agtattaaaa cgcggttcta tgttcgcgat gcgtgcgaag 1200

aaactgtatg acttgtatgt ggcttatgac tcgattgaag atatcccagc tgctgaacgt 1260

gagaagattg aaaaacaaat cttccgtgca aacctagacg agatttggga tggcactatc 1320

gctttcttta ctgaacgcga tccagaaatg ctagcccgtg caacgagtag tcctaaacgt 1380

aaaatggcac ttatcttccg ttggtatctt ggcctttctt cacgctggtc aaacacaggc 1440

gagaagggac gtgaaatgga ttatcagatt tgggcaggcc caagtttagg tgcattcaac 1500

agctgggtga aaggttctta ccttgaagac tatacccgcc gtggcgctgt agatgttgct 1560

ttgcatatgc ttaaaggtgc tgcgtattta caacgtgtaa accagttgaa attgcaaggt 1620

gttagcttaa gtacagaatt ggcaagttat cgtacgagtg attaa 1665

81

2910

Shewanella putrefaciens

81

atgagtatgt ttttaaattc aaaactttcg cgctcagtca aacttgccat atccgcaggc 60

ttaacagcct cgctagctat gcctgttttt gcagaagaaa ctgctgctga agaacaaata 120

gaaagagtcg cagtgaccgg atcgcgaatc gctaaagcag agctaactca accagctcca 180

gtcgtcagcc tttcagccga agaactgaca aaatttggta atcaagattt aggtagcgta 240

ctagcagaat tacctgctat tggtgcaacc aacactatta ttggtaataa caatagcaac 300

tcaagcgcag gtgttagctc agcagacttg cgtcgtctag gtgctaacag aaccttagta 360

ttagtcaacg gtaagcgcta cgttgccggc caaccgggct cagctgaggt agatttgtca 420

actataccaa ctagcatgat ctcgcgagtt gagattgtaa ccggcggtgc ttcagcaatt 480

tatggttcgg acgctgtatc aggtgttatc aacgttatcc ttaaagaaga ctttgaaggc 540

tttgagttta acgcacgtac tagcggttct actgaaagtg taggcactca agagcactct 600

tttgacattt tgggtggtgc aaacgttgca gatggacgtg gtaatgtaac cttctacgca 660

ggttatgaac gtacaaaaga agtcatggct accgacattc gccaattcga tgcttgggga 720

acaattaaaa acgaagccga tggtggtgaa gatgatggta ttccagacag actacgtgta 780

ccacgagttt attctgaaat gattaatgct accggtgtta tcaatgcatt tggtggtgga 840

attggtcgct caacctttga cagtaacggc aatcctattg cacaacaaga acgtgatggg 900

actaacagct ttgcatttgg ttcattccct aatggctgtg acacatgttt caacactgaa 960

gcatacgaaa actatattcc aggggtagaa agaataaacg ttggctcatc attcaacttt 1020

gattttaccg ataacattca attttacact gacttcagat atgtaaagtc agatattcag 1080

caacaatttc agccttcatt ccgttttggt aacattaata tcaatgttga agataacgcc 1140

tttttgaatg acgacttgcg tcagcaaatg ctcgatgcgg gtcaaaccaa tgctagtttt 1200

gccaagtttt ttgatgaatt aggaaatcgc tcagcagaaa ataaacgcga acttttccgt 1260

tacgtaggtg gctttaaagg tggctttgat attagcgaaa ccatatttga ttacgacctt 1320

tactatgttt atggcgagac taataaccgt cgtaaaaccc ttaatgacct aattcctgat 1380

aactttgtcg cagctgtcga ctctgttatt gatcctgata ctggcttagc agcgtgtcgc 1440

tcacaagtag caagcgctca aggcgatgac tatacagatc ccgcgtctgt aaatggtagc 1500

gactgtgttg cttataaccc atttggcatg ggtcaagctt cagcagaagc ccgcgactgg 1560

gtttctgctg atgtgactcg tgaagacaaa ataactcaac aagtgattgg tggtactctc 1620

ggtaccgatt ctgaagaact atttgagctt caaggtggtg caatcgctat ggttgttggt 1680

tttgaatacc gtgaagaaac gtctggttca acaaccgatg aatttactaa agcaggtttc 1740

ttgacaagcg ctgcaacgcc agattcttat ggcgaatacg acgtgactga gtattttgtt 1800

gaggtgaaca tcccagtact aaaagaatta ccttttgcac atgagttgag ctttgacggt 1860

gcataccgta atgctgatta ctcacatgcc ggtaagactg aagcatggaa agctggtatg 1920

ttctactcac cattagagca acttgcatta cgtggtacgg taggtgaagc agtacgagca 1980

ccaaacattg cagaagcctt tagtccacgc tctcctggtt ttggccgcgt ttcagatcca 2040

tgtgatgcag ataacattaa tgacgatccg gatcgcgtgt caaactgtgc agcattgggg 2100

atccctccag gattccaagc taatgataac gtcagtgtag ataccttatc tggtggtaac 2160

ccagatctaa aacctgaaac atcaacatcc tttacaggtg gtcttgtttg gacaccaacg 2220

tttgctgaca atctatcatt cactgtcgat tattatgata ttcaaattga ggatgctatt 2280

ttgtcagtag ccacccagac tgtggctgat aactgtgttg actcaactgg cggacctgac 2340

accgacttct gtagtcaagt tgatcgtaat ccaacgacct atgatattga acttgttcgc 2400

tctggttatc taaatgccgc ggcattgaat accaaaggta ttgaatttca agctgcatac 2460

tcattagatc tagagtcttt caacgcgcct ggtgaactac gcttcaacct attggggaac 2520

caattacttg aactagaacg tcttgaattc caaaatcgtc ctgatgagat taatgatgaa 2580

aaaggcgaag taggtgatcc agagctgcag ttccgcctag gcatcgatta ccgtctagat 2640

gatctaagtg ttagctggaa cacgcgttat attgatagcg tagtaactta tgatgtctct 2700

gaaaatggtg gctctcctga agatttatat ccaggccaca taggctcaat gacaactcat 2760

gacttgagcg ctacatacta catcaatgag aacttcatga ttaacggtgg tgtacgtaac 2820

ctatttgacg cacttccacc tggatacact aacgatgcgc tatatgatct agttggtcgc 2880

cgtgcattcc taggtattaa ggtaatgatg 2910

82

864

Shewanella putrefaciens

82

atggcaaaaa taaatagtga acacttggat gaagctacta ttacttcgaa taagtgtacg 60

caaacagaga ctgaggctcg gcatagaaat gccactacaa cacctgagat gcgccgattc 120

atacaagagt cggatctcag tgttagccaa ctgtctaaaa tattaaatat cagtgaagct 180

accgtacgta agtggcgcaa gcgtgactct gtcgaaaact gtcctaatac cccgcaccat 240

ctcaatacca cgctaacccc tttgcaagaa tatgtggttg tgggcctgcg ttatcaattg 300

aaaatgccat tagacagatt gctcaaagca acccaagagt ttatcaatcc aaacgtgtcg 360

cgctcaggtt tagcaagatg tttgaagcgt tatggcgttt cacgggtgag tgatatccaa 420

agcccacacg taccaatgcg ctactttaat caaattccag tcactcaagg cagcgatgtg 480

caaacctaca ccctgcacta tgaaacgctg gcaaaaacct tagccttacc tagtaccgat 540

ggtgacaatg tggtgcaagt ggtgtctctc accattccac caaagttaac cgaagaagca 600

cccagttcaa ttttgctcgg cattgatcct catagcgact ggatctatct cgacatatac 660

caagatggca atacacaagc cacgaataga tatatggctt atgtgctaaa acacgggcca 720

ttccatttac gaaagttact cgtgcgtaac tatcacacct ttttacagcg ctttcctgga 780

gcgacgcaaa atcgccgccc ctctaaagat atgcctgaaa caatcaacaa gacgcctgaa 840

acacaggcac ccagtggaga ctca 864

83

8268

Shewanella putrefaciens

83

atgagccaga cctctaaacc tacaaactca gcaactgagc aagcacaaga ctcacaagct 60

gactctcgtt taaataaacg actaaaagat atgccaattg ctattgttgg catggcgagt 120

atttttgcaa actctcgcta tttgaataag ttttgggact taatcagcga aaaaattgat 180

gcgattactg aattaccatc aactcactgg cagcctgaag aatattacga cgcagataaa 240

accgcagcag acaaaagcta ctgtaaacgt ggtggctttt tgccagatgt agacttcaac 300

ccaatggagt ttggcctgcc gccaaacatt ttggaactga ccgattcatc gcaactatta 360

tcactcatcg ttgctaaaga agtgttggct gatgctaact tacctgagaa ttacgaccgc 420

gataaaattg gtatcacctt aggtgtcggc ggtggtcaaa aaattagcca cagcctaaca 480

gcgcgtctgc aatacccagt attgaagaaa gtattcgcca atagcggcat tagtgacacc 540

gacagcgaaa tgcttatcaa gaaattccaa gaccaatatg tacactggga agaaaactcg 600

ttcccaggtt cacttggtaa cgttattgcg ggccgtatcg ccaaccgctt cgattttggc 660

ggcatgaact gtgtggttga tgctgcctgt gctggatcac ttgctgctat gcgtatggcg 720

ctaacagagc taactgaagg tcgctctgaa atgatgatca ccggtggtgt gtgtactgat 780

aactcaccct ctatgtatat gagcttttca aaaacgcccg cctttaccac taacgaaacc 840

attcagccat ttgatatcga ctcaaaaggc atgatgattg gtgaaggtat tggcatggtg 900

gcgctaaagc gtcttgaaga tgcagagcgc gatggcgacc gcatttactc tgtaattaaa 960

ggtgtgggtg catcatctga cggtaagttt aaatcaatct atgcccctcg cccatcaggc 1020

caagctaaag cacttaaccg tgcctatgat gacgcaggtt ttgcgccgca taccttaggt 1080

ctaattgaag ctcacggaac aggtactgca gcaggtgacg cggcagagtt tgccggcctt 1140

tgctcagtat ttgctgaagg caacgatacc aagcaacaca ttgcgctagg ttcagttaaa 1200

tcacaaattg gtcatactaa atcaactgca ggtacagcag gtttaattaa agctgctctt 1260

gctttgcatc acaaggtact gccgccgacc attaacgtta gtcagccaag ccctaaactt 1320

gatatcgaaa actcaccgtt ttatctaaac actgagactc gtccatggtt accacgtgtt 1380

gatggtacgc cgcgccgcgc gggtattagc tcatttggtt ttggtggcac taacttccat 1440

tttgtactag aagagtacaa ccaagaacac agccgtactg atagcgaaaa agctaagtat 1500

cgtcaacgcc aagtggcgca aagcttcctt gttagcgcaa gcgataaagc atcgctaatt 1560

aacgagttaa acgtactagc agcatctgca agccaagctg agtttatcct caaagatgca 1620

gcagcaaact atggcgtacg tgagcttgat aaaaatgcac cacggatcgg tttagttgca 1680

aacacagctg aagagttagc aggcctaatt aagcaagcac ttgccaaact agcagctagc 1740

gatgataacg catggcagct acctggtggc actagctacc gcgccgctgc agtagaaggt 1800

aaagttgccg cactgtttgc tggccaaggt tcacaatatc tcaatatggg ccgtgacctt 1860

acttgttatt acccagagat gcgtcagcaa tttgtaactg cagataaagt atttgccgca 1920

aatgataaaa cgccgttatc gcaaactctg tatccaaagc ctgtatttaa taaagatgaa 1980

ttaaaggctc aagaagccat tttgaccaat accgccaatg cccaaagcgc aattggtgcg 2040

atttcaatgg gtcaatacga tttgtttact gcggctggct ttaatgccga catggttgca 2100

ggccatagct ttggtgagct aagtgcactg tgtgctgcag gtgttatttc agctgatgac 2160

tactacaagc tggcttttgc tcgtggtgag gctatggcaa caaaagcacc ggctaaagac 2220

ggcgttgaag cagatgcagg agcaatgttt gcaatcataa ccaagagtgc tgcagacctt 2280

gaaaccgttg aagccaccat cgctaaattt gatggggtga aagtcgctaa ctataacgcg 2340

ccaacgcaat cagtaattgc aggcccaaca gcaactaccg ctgatgcggc taaagcgcta 2400

actgagcttg gttacaaagc gattaacctg ccagtatcag gtgcattcca cactgaactt 2460

gttggtcacg ctcaagcgcc atttgctaaa gcgattgacg cagccaaatt tactaaaaca 2520

agccgagcac tttactcaaa tgcaactggc ggactttatg aaagcactgc tgcaaagatt 2580

aaagcctcgt ttaagaaaca tatgcttcaa tcagtgcgct ttactagcca gctagaagcc 2640

atgtacaacg acggcgcccg tgtatttgtt gaatttggtc caaagaacat cttacaaaaa 2700

ttagttcaag gcacgcttgt caacactgaa aatgaagttt gcactatctc tatcaaccct 2760

aatcctaaag ttgatagtga tctgcagctt aagcaagcag caatgcagct agcggttact 2820

ggtgtggtac tcagtgaaat tgacccatac caagccgata ttgccgcacc agcgaaaaag 2880

tcgccaatga gcatttcgct taatgctgct aaccatatca gcaaagcaac tcgcgctaag 2940

atggccaagt ctttagagac aggtatcgtc acctcgcaaa tagaacatgt tattgaagaa 3000

aaaatcgttg aagttgagaa actggttgaa gtcgaaaaga tcgtcgaaaa agtggttgaa 3060

gtagagaaag ttgttgaggt tgaagctcct gttaattcag tgcaagccaa tgcaattcaa 3120

acccgttcag ttgtcgctcc agtaatagag aaccaagtcg tgtctaaaaa cagtaagcca 3180

gcagtccaga gcattagtgg tgatgcactc agcaactttt ttgctgcaca gcagcaaacc 3240

gcacagttgc atcagcagtt cttagctatt ccgcagcaat atggtgagac gttcactacg 3300

ctgatgaccg agcaagctaa actggcaagt tctggtgttg caattccaga gagtctgcaa 3360

cgctcaatgg agcaattcca ccaactacaa gcgcaaacac tacaaagcca cacccagttc 3420

cttgagatgc aagcgggtag caacattgca gcgttaaacc tactcaatag cagccaagca 3480

acttacgctc cagccattca caatgaagcg attcaaagcc aagtggttca aagccaaact 3540

gcagtccagc cagtaatttc aacacaagtt aaccatgtgt cagagcagcc aactcaagct 3600

ccagctccaa aagcgcagcc agcacctgtg acaactgcag ttcaaactgc tccggcacaa 3660

gttgttcgtc aagccgcacc agttcaagcc gctattgaac cgattaatac aagtgttgcg 3720

actacaacgc cttcagcctt cagcgccgaa acagccctga gcgcaacaaa agtccaagcc 3780

actatgcttg aagtggttgc tgagaaaacc ggttacccaa ctgaaatgct agagcttgaa 3840

atggatatgg aagccgattt aggcatcgat tctatcaagc gtgtagaaat tcttggcaca 3900

gtacaagatg agctaccggg tctacctgag cttagccctg aagatctagc tgagtgtcga 3960

acgctaggcg aaatcgttga ctatatgggc agtaaactgc cggctgaagg ctctatgaat 4020

tctcagctgt ctacaggttc cgcagctgcg actcctgcag cgaatggtct ttctgcggag 4080

aaagttcaag cgactatgat gtctgtggtt gccgaaaaga ctggctaccc aactgaaatg 4140

ctagagcttg aaatggatat ggaagccgat ttaggcatag attctatcaa gcgcgttgaa 4200

attcttggca cagtacaaga tgagctaccg ggtctacctg agcttagccc tgaagatcta 4260

gctgagtgtc gtactctagg cgaaatcgtt gactatatga actctaaact cgctgacggc 4320

tctaagctgc cggctgaagg ctctatgaat tctcagctgt ctacaagtgc cgcagctgcg 4380

actcctgcag cgaatggtct ctctgcggag aaagttcaag cgactatgat gtctgtggtt 4440

gccgaaaaga ctggctaccc aactgaaatg ctagaacttg aaatggatat ggaagctgac 4500

cttggcatcg attcaatcaa gcgcgttgaa attcttggca cagtacaaga tgagctaccg 4560

ggtttacctg agctaaatcc agaagatttg gcagagtgtc gtactcttgg cgaaatcgtg 4620

acttatatga actctaaact cgctgacggc tctaagctgc cagctgaagg ctctatgcac 4680

tatcagctgt ctacaagtac cgctgctgcg actcctgtag cgaatggtct ctctgcagaa 4740

aaagttcaag cgaccatgat gtctgtagtt gcagataaaa ctggctaccc aactgaaatg 4800

cttgaacttg aaatggatat ggaagccgat ttaggtatcg attctatcaa gcgcgttgaa 4860

attcttggca cagtacaaga tgagctaccg ggtttacctg agctaaatcc agaagatcta 4920

gcagagtgtc gcaccctagg cgaaatcgtt gactatatgg gcagtaaact gccggctgaa 4980

ggctctgcta atacaagtgc cgctgcgtct cttaatgtta gtgccgttgc ggcgcctcaa 5040

gctgctgcga ctcctgtatc gaacggtctc tctgcagaga aagtgcaaag cactatgatg 5100

tcagtagttg cagaaaagac cggctaccca actgaaatgc tagaacttgg catggatatg 5160

gaagccgatt taggtatcga ctcaattaaa cgcgttgaga ttcttggcac agtacaagat 5220

gagctaccgg gtctaccaga gcttaatcct gaagatttag ctgagtgccg tacgctgggc 5280

gaaatcgttg actatatgaa ctctaagctg gctgacggct ctaagcttcc agctgaaggc 5340

tctgctaata caagtgccac tgctgcgact cctgcagtga atggtctttc tgctgacaag 5400

gtacaggcga ctatgatgtc tgtagttgct gaaaagaccg gctacccaac tgaaatgcta 5460

gaacttggca tggatatgga agcagacctt ggtattgatt ctattaagcg cgttgaaatt 5520

cttggcacag tacaagatga gctcccaggt ttacctgagc ttaatcctga agatctcgct 5580

gagtgccgca cgcttggcga aatcgttagc tatatgaact ctcaactggc tgatggctct 5640

aaactttcta caagtgcggc tgaaggctct gctgatacaa gtgctgcaaa tgctgcaaag 5700

ccggcagcaa tttcggcaga accaagtgtt gagcttcctc ctcatagcga ggtagcgcta 5760

aaaaagctta atgcggcgaa caagctagaa aattgtttcg ccgcagacgc aagtgttgtg 5820

attaacgatg atggtcacaa cgcaggcgtt ttagctgaga aacttattaa acaaggccta 5880

aaagtagccg ttgtgcgttt accgaaaggt cagcctcaat cgccactttc aagcgatgtt 5940

gctagctttg agcttgcctc aagccaagaa tctgagcttg aagccagtat cactgcagtt 6000

atcgcgcaga ttgaaactca ggttggcgct attggtggct ttattcactt gcaaccagaa 6060

gcgaatacag aagagcaaac ggcagtaaac ctagatgcgc aaagttttac tcacgttagc 6120

aatgcgttct tgtgggccaa attattgcaa ccaaagctcg ttgctggagc agatgcgcgt 6180

cgctgttttg taacagtaag ccgtatcgac ggtggctttg gttacctaaa tactgacgcc 6240

ctaaaagatg ctgagctaaa ccaagcagca ttagctggtt taactaaaac cttaagccat 6300

gaatggccac aagtgttctg tcgcgcgcta gatattgcaa cagatgttga tgcaacccat 6360

cttgctgatg caatcaccag tgaactattt gatagccaag ctcagctacc tgaagtgggc 6420

ttaagcttaa ttgatggcaa agttaaccgc gtaactctag ttgctgctga agctgcagat 6480

aaaacagcaa aagcagagct taacagcaca gataaaatct tagtgactgg tggggcaaaa 6540

ggggtgacat ttgaatgtgc actggcatta gcatctcgca gccagtctca ctttatctta 6600

gctgggcgca gtgaattaca agctttacca agctgggctg agggtaagca aactagcgag 6660

ctaaaatcag ctgcaatcgc acatattatt tctactggtc aaaagccaac gcctaagcaa 6720

gttgaagccg ctgtgtggcc agtgcaaagc agcattgaaa ttaatgccgc cctagccgcc 6780

tttaacaaag ttggcgcctc agctgaatac gtcagcatgg atgttaccga tagcgccgca 6840

atcacagcag cacttaatgg tcgctcaaat gagatcaccg gtcttattca tggcgcaggt 6900

gtactagccg acaagcatat tcaagacaag actcttgctg aacttgctaa agtttatggc 6960

actaaagtca acggcctaaa agcgctgctc gcggcacttg agccaagcaa aattaaatta 7020

cttgctatgt tctcatctgc agcaggtttt tacggtaata tcggccaaag cgattacgcg 7080

atgtcgaacg atattcttaa caaggcagcg ctgcagttca ccgctcgcaa cccacaagct 7140

aaagtcatga gctttaactg gggtccttgg gatggcggca tggttaaccc agcgcttaaa 7200

aagatgttta ccgagcgtgg tgtgtacgtt attccactaa aagcaggtgc agagctattt 7260

gccactcagc tattggctga aactggcgtg cagttgctca ttggtacgtc aatgcaaggt 7320

ggcagcgaca ctaaagcaac tgagactgct tctgtaaaaa agcttaatgc gggtgaggtg 7380

ctaagtgcat cgcatccgcg tgctggtgca caaaaaacac cactacaagc tgtcactgca 7440

acgcgtctgt taaccccaag tgccatggtc ttcattgaag atcaccgcat tggcggtaac 7500

agtgtgttgc caacggtatg cgccatcgac tggatgcgtg aagcggcaag cgacatgctt 7560

ggcgctcaag ttaaggtact tgattacaag ctattaaaag gcattgtatt tgagactgat 7620

gagccgcaag agttaacact tgagctaacg ccagacgatt cagacgaagc tacgctacaa 7680

gcattaatca gctgtaatgg gcgtccgcaa tacaaggcga cgcttatcag tgataatgcc 7740

gatattaagc aacttaacaa gcagtttgat ttaagcgcta aggcgattac cacagcaaaa 7800

gagctttata gcaacggcac cttgttccac ggtccgcgtc tacaagggat ccaatctgta 7860

gtgcagttcg atgatcaagg cttaattgct aaagtcgctc tgcctaaggt tgaacttagc 7920

gattgtggtg agttcttgcc gcaaacccac atgggtggca gtcaaccttt tgctgaggac 7980

ttgctattac aagctatgct ggtttgggct cgccttaaaa ctggctcggc aagtttgcca 8040

tcaagcattg gtgagtttac ctcataccaa ccaatggcct ttggtgaaac tggtaccata 8100

gagcttgaag tgattaagca caacaaacgc tcacttgaag cgaatgttgc gctatatcgt 8160

gacaacggcg agttaagtgc catgtttaag tcagctaaaa tcaccattag caaaagctta 8220

aattcagcat ttttacctgc tgtcttagca aacgacagtg aggcgaat 8268

84

2313

Shewanella putrefaciens

84

atgccgctgc gcatcgcact tatcttactg ccaacaccgc agtttgaagt taactctgtc 60

gaccagtcag tattagccag ctatcaaaca ctgcagcctg agctaaatgc cctgcttaat 120

agtgcgccga cacctgaaat gctcagcatc actatctcag atgatagcga tgcaaacagc 180

tttgagtcgc agctaaatgc tgcgaccaac gcaattaaca atggctatat cgtcaagctt 240

gctacggcaa ctcacgcttt gttaatgctg cctgcattaa aagcggcgca aatgcggatc 300

catcctcatg cgcagcttgc cgctatgcag caagctaaat cgacgccaat gagtcaagta 360

tctggtgagc taaagcttgg cgctaatgcg ctaagcctag ctcagactaa tgcgctgtct 420

catgctttaa gccaagccaa gcgtaactta actgatgtca gcgtgaatga gtgttttgag 480

aacctcaaaa gtgaacagca gttcacagag gtttattcgc ttattcagca acttgctagc 540

cgcacccatg tgagaaaaga ggttaatcaa ggtgtggaac ttggccctaa acaagccaaa 600

agccactatt ggtttagcga atttcaccaa aaccgtgttg ctgccatcaa ctttattaat 660

ggccaacaag caaccagcta tgtgcttact caaggttcag gattgttagc tgcgaaatca 720

atgctaaacc agcaaagatt aatgtttatc ttgccgggta acagtcagca acaaataacc 780

gcatcaataa ctcagttaat gcagcaatta gagcgtttgc aggtaactga ggttaatgag 840

ctttctctag aatgccaact agagctgctc agcataatgt atgacaactt agtcaacgca 900

gacaaactca ctactcgcga tagtaagccc gcttatcagg ctgtgattca agcaagctct 960

gttagcgctg caaagcaaga gttaagcgcg cttaacgatg cactcacagc gctgtttgct 1020

gagcaaacaa acgccacatc aacgaataaa ggcttaatcc aatacaaaac accggcgggc 1080

agttacttaa ccctaacacc gcttggcagc aacaatgaca acgcccaagc gggtcttgct 1140

tttgtctatc cgggtgtggg aacggtttac gccgatatgc ttaatgagct gcatcagtac 1200

ttccctgcgc tttacgccaa acttgagcgt gaaggcgatt taaaggcgat gctacaagca 1260

gaagatatct atcatcttga ccctaaacat gctgcccaaa tgagcttagg tgacttagcc 1320

attgctggcg tggggagcag ctacctgtta actcagctgc tcaccgatga gtttaatatt 1380

aagcctaatt ttgcattagg ttactcaatg ggtgaagcat caatgtgggc aagcttaggc 1440

gtatggcaaa acccgcatgc gctgatcagc aaaacccaaa ccgacccgct atttacttct 1500

gctatttccg gcaaattgac cgcggttaga caagcttggc agcttgatga taccgcagcg 1560

gaaatccagt ggaatagctt tgtggttaga agtgaagcag cgccgattga agccttgcta 1620

aaagattacc cacacgctta cctcgcgatt attcaagggg atacctgcgt aatcgctggc 1680

tgtgaaatcc aatgtaaagc gctacttgca gcactgggta aacgcggtat tgcagctaat 1740

cgtgtaacgg cgatgcatac gcagcctgcg atgcaagagc atcaaaatgt gatggatttt 1800

tatctgcaac cgttaaaagc agagcttcct agtgaaataa gctttatcag cgccgctgat 1860

ttaactgcca agcaaacggt gagtgagcaa gcacttagca gccaagtcgt tgctcagtct 1920

attgccgaca ccttctgcca aaccttggac tttaccgcgc tagtacatca cgcccaacat 1980

caaggcgcta agctgtttgt tgaaattggc gcggatagac aaaactgcac cttgatagac 2040

aagattgtta aacaagatgg tgccagcagt gtacaacatc aaccttgttg cacagtgcct 2100

atgaacgcaa aaggtagcca agatattacc agcgtgatta aagcgcttgg ccaattaatt 2160

agccatcagg tgccattatc ggtgcaacca tttattgatg gactcaagcg cgagctaaca 2220

ctttgccaat tgaccagcca acagctggca gcacatgcaa atgttgacag caagtttgag 2280

tctaaccaag accatttact tcaaggggaa gtc 2313

85

6012

Shewanella putrefaciens

85

atgtcattac cagacaatgc ttctaaccac ctttctgcca accagaaagg cgcatctcag 60

gcaagtaaaa ccagtaagca aagcaaaatc gccattgtcg gtttagccac tctgtatcca 120

gacgctaaaa ccccgcaaga attttggcag aatttgctgg ataaacgcga ctctcgcagc 180

accttaacta acgaaaaact cggcgctaac agccaagatt atcaaggtgt gcaaggccaa 240

tctgaccgtt tttattgtaa taaaggcggc tacattgaga acttcagctt taatgctgca 300

ggctacaaat tgccggagca aagcttaaat ggcttggacg acagcttcct ttgggcgctc 360

gatactagcc gtaacgcact aattgatgct ggtattgata tcaacggcgc tgatttaagc 420

cgcgcaggtg tagtcatggg cgcgctgtcg ttcccaacta cccgctcaaa cgatctgttt 480

ttgccaattt atcacagcgc cgttgaaaaa gccctgcaag ataaactagg cgtaaaggca 540

tttaagctaa gcccaactaa tgctcatacc gctcgcgcgg caaatgagag cagcctaaat 600

gcagccaatg gtgccattgc ccataacagc tcaaaagtgg tggccgatgc acttggcctt 660

ggcggcgcac aactaagcct agatgctgcc tgtgctagtt cggtttactc attaaagctt 720

gcctgcgatt acctaagcac tggcaaagcc gatatcatgc tagcaggcgc agtatctggc 780

gcggatcctt tctttattaa tatgggattc tcaatcttcc acgcctaccc agaccatggt 840

atctcagtac cgtttgatgc cagcagtaaa ggtttgtttg ctggcgaagg cgctggcgta 900

ttagtgctta aacgtcttga agatgccgag cgcgacaatg acaaaatcta tgcggttgtt 960

agcggcgtag gtctatcaaa cgacggtaaa ggccagtttg tattaagccc taatccaaaa 1020

ggtcaggtga aggcctttga acgtgcttat gctgccagtg acattgagcc aaaagacatt 1080

gaagtgattg agtgccacgc aacaggcaca ccgcttggcg ataaaattga gctcacttca 1140

atggaaacct tctttgaaga caagctgcaa ggcaccgatg caccgttaat tggctcagct 1200

aagtctaact taggccacct attaactgca gcgcatgcgg ggatcatgaa gatgatcttc 1260

gccatgaaag aaggttacct gccgccaagt atcaatatta gtgatgctat cgcttcgccg 1320

aaaaaactct tcggtaaacc aaccctgcct agcatggttc aaggctggcc agataagcca 1380

tcgaataatc attttggtgt aagaacccgt cacgcaggcg tatcggtatt tggctttggt 1440

ggctgtaacg cccatctgtt gcttgagtca tacaacggca aaggaacagt aaaggcagaa 1500

gccactcaag taccgcgtca agctgagccg ctaaaagtgg ttggccttgc ctcgcacttt 1560

gggcctctta gcagcattaa tgcactcaac aatgctgtga cccaagatgg gaatggcttt 1620

atcgaactgc cgaaaaagcg ctggaaaggc cttgaaaagc acagtgaact gttagctgaa 1680

tttggcttag catctgcgcc aaaaggtgct tatgttgata acttcgagct ggacttttta 1740

cgctttaaac tgccgccaaa cgaagatgac cgtttgatct cacagcagct aatgctaatg 1800

cgagtaacag acgaagccat tcgtgatgcc aagcttgagc cggggcaaaa agtagctgta 1860

ttagtggcaa tggaaactga gcttgaactg catcagttcc gcggccgggt taacttgcat 1920

actcaattag cgcaaagtct tgccgccatg ggcgtgagtt tatcaacgga tgaataccaa 1980

gcgcttgaag ccatcgccat ggacagcgtg cttgatgctg ccaagctcaa tcagtacacc 2040

agctttattg gtaatattat ggcgtcacgc gtggcgtcac tatgggactt taatggccca 2100

gccttcacta tttcagcagc agagcaatct gtgagccgct gtatcgatgt ggcgcaaaac 2160

ctcatcatgg aggataacct agatgcggtg gtgattgcag cggtcgatct ctctggtagc 2220

tttgagcaag tcattcttaa aaatgccatt gcacctgtag ccattgagcc aaacctcgaa 2280

gcaagcctta atccaacatc agcaagctgg aatgtcggtg aaggtgctgg cgcggtcgtg 2340

cttgttaaaa atgaagctac atcgggctgc tcatacggcc aaattgatgc acttggcttt 2400

gctaaaactg ccgaaacagc gttggctacc gacaagctac tgagccaaac tgccacagac 2460

tttaataagg ttaaagtgat tgaaactatg gcagcgcctg ctagccaaat tcaattagcg 2520

ccaatagtta gctctcaagt gactcacact gctgcagagc agcgtgttgg tcactgcttt 2580

gctgcagcgg gtatggcaag cctattacac ggcttactta acttaaatac tgtagcccaa 2640

accaataaag ccaattgcgc gcttatcaac aatatcagtg aaaaccaatt atcacagctg 2700

ttgattagcc aaacagcgag cgaacaacaa gcattaaccg cgcgtttaag caatgagctt 2760

aaatccgatg ctaaacacca actggttaag caagtcacct taggtggccg tgatatctac 2820

cagcatattg ttgatacacc gcttgcaagc cttgaaagca ttactcagaa attggcgcaa 2880

gcgacagcat cgacagtggt caaccaagtt aaacctatta aggccgctgg ctcagtcgaa 2940

atggctaact cattcgaaac ggaaagctca gcagagccac aaataacaat tgcagcacaa 3000

cagactgcaa acattggcgt caccgctcag gcaaccaaac gtgaattagg taccccacca 3060

atgacaacaa ataccattgc taatacagca aataatttag acaagactct tgagactgtt 3120

gctggcaata ctgttgctag caaggttggc tctggcgaca tagtcaattt tcaacagaac 3180

caacaattgg ctcaacaagc tcacctcgcc tttcttgaaa gccgcagtgc gggtatgaag 3240

gtggctgatg ctttattgaa gcaacagcta gctcaagtaa caggccaaac tatcgataat 3300

caggccctcg atactcaagc cgtcgatact caaacaagcg agaatgtagc gattgccgca 3360

gaatcaccag ttcaagttac aacacctgtt caagttacaa cacctgttca aatcagtgtt 3420

gtggagttaa aaccagatca cgctaatgtg ccaccataca cgccgccagt gcctgcatta 3480

aagccgtgta tctggaacta tgccgattta gttgagtacg cagaaggcga tatcgccaag 3540

gtatttggca gtgattatgc cattatcgac agctactcgc gccgcgtacg tctaccgacc 3600

actgactacc tgttggtatc gcgcgtgacc aaacttgatg cgaccatcaa tcaatttaag 3660

ccatgctcaa tgaccactga gtacgacatc cctgttgatg cgccgtactt agtagacgga 3720

caaatccctt gggcggtagc agtagaatca ggccaatgtg acttgatgct tattagctat 3780

ctcggtatcg actttgagaa caaaggcgag cgggtttatc gactactcga ttgtaccctc 3840

accttcctag gcgacttgcc acgtggcgga gataccctac gttacgacat taagatcaat 3900

aactatgctc gcaacggcga caccctgctg ttcttcttct cgtatgagtg ttttgttggc 3960

gacaagatga tcctcaagat ggatggcggc tgcgctggct tcttcactga tgaagagctt 4020

gccgacggta aaggcgtgat tcgcacagaa gaagagatta aagctcgcag cctagtgcaa 4080

aagcaacgct ttaatccgtt actagattgt cctaaaaccc aatttagtta tggtgatatt 4140

cataagctat taactgctga tattgagggt tgttttggcc caagccacag tggcgtccac 4200

cagccgtcac tttgtttcgc atctgaaaaa ttcttgatga ttgaacaagt cagcaaggtt 4260

gatcgcactg gcggtacttg gggacttggc ttaattgagg gtcataagca gcttgaagca 4320

gaccactggt acttcccatg tcatttcaag ggcgaccaag tgatggctgg ctcgctaatg 4380

gctgaaggtt gtggccagtt attgcagttc tatatgctgc accttggtat gcatacccaa 4440

actaaaaatg gtcgtttcca acctcttgaa aacgcctcac agcaagtacg ctgtcgcggt 4500

caagtgctgc cacaatcagg cgtgctaact taccgtatgg aagtgactga aatcggtttc 4560

agtccacgcc catatgctaa agctaacatc gatatcttgc ttaatggcaa agcggtagtg 4620

gatttccaaa acctaggggt gatgataaaa gaggaagatg agtgtactcg ttatccactt 4680

ttgactgaat caacaacggc tagcactgca caagtaaacg ctcaaacaag tgcgaaaaag 4740

gtatacaagc cagcatcagt caatgcgcca ttaatggcac aaattcctga tctgactaaa 4800

gagccaaaca agggcgttat tccgatttcc catgttgaag caccaattac gccagactac 4860

ccgaaccgtg tacctgatac agtgccattc acgccgtatc acatgtttga gtttgctaca 4920

ggcaatatcg aaaactgttt cgggccagag ttctcaatct atcgcggcat gatcccacca 4980

cgtacaccat gcggtgactt acaagtgacc acacgtgtga ttgaagttaa cggtaagcgt 5040

ggcgacttta aaaagccatc atcgtgtatc gctgaatatg aagtgcctgc agatgcgtgg 5100

tatttcgata aaaacagcca cggcgcagtg atgccatatt caattttaat ggagatctca 5160

ctgcaaccta acggctttat ctcaggttac atgggcacaa ccctaggctt ccctggcctt 5220

gagctgttct tccgtaactt agacggtagc ggtgagttac tacgtgaagt agatttacgt 5280

ggtaaaacca tccgtaacga ctcacgttta ttatcaacag tgatggccgg cactaacatc 5340

atccaaagct ttagcttcga gctaagcact gacggtgagc ctttctatcg cggcactgcg 5400

gtatttggct attttaaagg tgacgcactt aaagatcagc taggcctaga taacggtaaa 5460

gtcactcagc catggcatgt agctaacggc gttgctgcaa gcactaaggt gaacctgctt 5520

gataagagct gccgtcactt taatgcgcca gctaaccagc cacactatcg tctagccggt 5580

ggtcagctga actttatcga cagtgttgaa attgttgata atggcggcac cgaaggttta 5640

ggttacttgt atgccgagcg caccattgac ccaagtgatt ggttcttcca gttccacttc 5700

caccaagatc cggttatgcc aggctcctta ggtgttgaag caattattga aaccatgcaa 5760

gcttacgcta ttagtaaaga cttgggcgca gatttcaaaa atcctaagtt tggtcagatt 5820

ttatcgaaca tcaagtggaa gtatcgcggt caaatcaatc cgctgaacaa gcagatgtct 5880

atggatgtca gcattacttc aatcaaagat gaagacggta agaaagtcat cacaggtaat 5940

gccagcttga gtaaagatgg tctgcgcata tacgaggtct tcgatatagc tatcagcatc 6000

gaagaatctg ta 6012

86

1629

Shewanella putrefaciens

86

atgaatccta cagcaactaa cgaaatgctt tctccgtggc catgggctgt gacagagtca 60

aatatcagtt ttgacgtgca agtgatggaa caacaactta aagattttag ccgggcatgt 120

tacgtggtca atcatgccga ccacggcttt ggtattgcgc aaactgccga tatcgtgact 180

gaacaagcgg caaacagcac agatttacct gttagtgctt ttactcctgc attaggtacc 240

gaaagcctag gcgacaataa tttccgccgc gttcacggcg ttaaatacgc ttattacgca 300

ggcgctatgg caaacggtat ttcatctgaa gagctagtga ttgccctagg tcaagctggc 360

attttgtgtg gttcgtttgg agcagccggt cttattccaa gtcgcgttga agcggcaatt 420

aaccgtattc aagcagcgct gccaaatggc ccttatatgt ttaaccttat ccatagtcct 480

agcgagccag cattagagcg tggcagcgta gagctatttt taaagcataa ggtacgcacc 540

gttgaagcat cagctttctt aggtctaaca ccacaaatcg tctattaccg tgcagcagga 600

ttgagccgag acgcacaagg taaagttgtg gttggtaaca aggttatcgc taaagtaagt 660

cgcaccgaag tggctgaaaa gtttatgatg ccagcgcccg caaaaatgct acaaaaacta 720

gttgatgacg gttcaattac cgctgagcaa atggagctgg cgcaacttgt acctatggct 780

gacgacatca ctgcagaggc cgattcaggt ggccatactg ataaccgtcc attagtaaca 840

ttgctgccaa ccattttagc gctgaaagaa gaaattcaag ctaaatacca atacgacact 900

cctattcgtg tcggttgtgg tggcggtgtg ggtacgcctg atgcagcgct ggcaacgttt 960

aacatgggcg cggcgtatat tgttaccggc tctatcaacc aagcttgtgt tgaagcgggc 1020

gcaagtgatc acactcgtaa attacttgcc accactgaaa tggccgatgt gactatggca 1080

ccagctgcag atatgttcga gatgggcgta aaactgcagg tggttaagcg cggcacgcta 1140

ttcccaatgc gcgctaacaa gctatatgag atctacaccc gttacgattc aatcgaagcg 1200

atcccattag acgagcgtga aaagcttgag aaacaagtat tccgctcaag cctagatgaa 1260

atatgggcag gtacagtggc gcactttaac gagcgcgacc ctaagcaaat cgaacgcgca 1320

gagggtaacc ctaagcgtaa aatggcattg attttccgtt ggtacttagg tctttctagt 1380

cgctggtcaa actcaggcga agtgggtcgt gaaatggatt atcaaatttg ggctggccct 1440

gctctcggtg catttaacca atgggcaaaa ggcagttact tagataacta tcaagaccga 1500

aatgccgtcg atttggcaaa gcacttaatg tacggcgcgg cttacttaaa tcgtattaac 1560

tcgctaacgg ctcaaggcgt taaagtgcca gcacagttac ttcgctggaa gccaaaccaa 1620

agaatggcc 1629