Method for determining the nucleotide sequence of the gene for the α5(IV) chain of human type IV collagen

This is a continuation of application Ser. No. 08/346,336 filed on Nov. 29, 1994, now abandoned which is a continuation of application Ser. No. 07/635,475 filed on Dec. 28, 1990, now abandoned, which is a continuation-in-part patent application to the parent U.S. application “Method for determining the nucleotide sequence of a novel α5(IV) chain of human type IV collagen” Ser. No.07/377,238, filed on Jul. 7, 1989 and issued on May 19, 1992 as U.S. Pat. No. 5,114,840.

The present invention relates to a method for isolating and Identifying the nucleotide sequence of the human gene for the type IV collagen a5(IV) chain. The present invention is directed to the determination of the nucleotide sequence of the gene for the a5(IV) collagen chain in individuals by any method known to the art e.g. cloning from genomic DNA libraries or amplifying gene regions with the polymerase chain reaction (PCR) and studying their physical properties or nucleotide sequences. In addition, the invention is directed to the use of the nucleotide sequences of the a5(IV) gene to amplify or identify the nucleotide sequences of the a5(IV) gene.

Basement membranes (BM) are special extracellular, sheet-like structures that separate cells of organs from the underlying connective tissues. They form flexible boundaries that provide physical support and biological signals required for maintainance of morphology and orderly development of distinct tissue patterns. The BM protein components can have different subunits and molecular compositions that possess the necessary functional elements for the tissues concerned. This has become more apparent as new chains with restricted tissue distributions have been found e.g. for type IV collagen and laminin. The basement membranes have also an Important role in the correct regeneration of tissues following injuries such as during post-wound reformation of skin and nerves. Basement membranes also function as macromolecular filters e.g. in kidneys where the glomerular basement membrane is the sole filtration barrier between the capillary lumen and the urinary space, hindering the blood to urine leakage of macromolecules and blood cells.

Basement membranes are composed of several specific components that include type IV collagen, laminin, entactin (nidogen) and proteoglycans. Type IV collagen is the major structural component of basement membranes and it forms the framework of these extracellular structures. In addition, basement membranes contain SPARK (BM-40), fibronectin and type VII collagen that are also present in other extracellular structures. The exact molecular compositions of basement membranes in different tissues Is not well known but there Is growing evidence that even the ubiquitous basement membrane components as type IV collagen and laminin have different chain compositions in different tissues. Additionally, there are some proteins such as pemphigoid antigen that are present only In the basement membranes of skin.

Type IV collagen is the major structural component of basement membranes and It can provide up to 60 % of the structure. As all collagens, the type IV collagen molecule is formed by three a chains coiled around each other to form the collagen triple helix with the repeated Gly-X-Y-triplet amino acid sequence containing regions. The molecule has a triple-helical 400 nm-long collagenous part and a C-terminal globule with a diameter of about 15 nm. The collagenous domain sequence has several interruptions in the otherwise continuous collagenous Gly-X-Y repeat sequence that give flexibility to the type IV collagen molecules as opposed to the rigid rod-like molecules of fibrillar collagens with uninterrupted helices. The triple-helical type IV collagen molecules can form dimers by the attachment of two NC domains and tetramers by the 30 nm overlapping cross-linking of four molecules of their amino terminal ends (Timpl, Eur. J. Biochem. 180: 487-502, 1989).

The major form of the molecules consists of two α1(IV) and one α2(IV) chain. The applicants have determined the entire amino acid sequence of these two chains from man by cloning and sequencing cDNA clones covering the coding region (Soininen et al., FEBS. Lett., 225: 188-194, 1987; Hostikka and Tryggvason, J. Biol. Chem., 263: 19488-19493, 1988). The results showed that the α1(IV) chain is synthesized as a 1969 amino acid residue polypeptide as compared to 1712 residues in the α2(IV) chain. The carboxyl terminal NC domains of the two chains are very similar with 63 % identical amino acid residues. The sequence identity of the two chains is much less conserved in the triple-helical region with only 49 % identity; where only 22 % of the X and Y residues in the collagenous Gly-X-Y-repeat sequence are conserved. Two other distinct type IV collagen α chains, referred to as α3(IV) and α4(IV), have been described (Butkowski et al., J. Biol. Chem., 262: 7874-7877, 1987; Saus et al. J. Biol. Chem. 263: 13374-13380, 1988; Gunwar et al., J. Biol. Chem., 265: 5466-5469, 1990).

Of importance with respect to the present invention is our recent discovery of yet another novel human type IV collagen α5(IV) chain by cDNA cloning (Hostikka et al., Proc. Natl. Acad. Sci. USA. 87: 1606-1610, 1990 and the parent U.S. patent application “Method for determining the nucleotide sequence of a novel α5(IV) chain of human type IV collagen” Ser. No. 377,238, filed on Jul.7, 1989 now U.S. Pat. No. 5,114,840. Amino acid sequence comparison with the α1(IV) and α2(IV) chains and the data available of the α3(IV) and α4(IV) chains demonstrated that the α5(IV) chain is a distinct gene product which is closely related to the α1(IV) chain. In the NC domain the identity between the deduced amino acid sequences is 83 % with the α1(IV) chain and with α2(IV) chain 63 %; whereas in the collagenous domain the identities are 58 % and 46 %, respectively. Furthermore, all the interruptions in the collagenous Gly-X-Y-repeat sequence of the α5(IV) chain coincide with those in the α1(IV) chain but only partially with those In the α2(IV) chain (Hostikka et al., Proc. Natl. Acad. Sci. USA. 87: 1606-1610, 1990 and the parent U.S. patent application “Method for determining the nucleotide sequence of a novel α5(IV) chain of human type IV collagen” Ser. No. 377,238,filed on Jul. 7, 1989, now U.S. Pat. No. 5,144,840.

With α5(IV)-specific peptide-antibodies, the chain was shown to be almost exclusively present in the GBM in the kidney (Hostikka et al., Proc. Natl. Acad. Sci. USA. 87: 1606-1610, 1990 and the parent U.S. patent application “Method for determining the nucleotide sequence of a novel α5(IV) chain of human type IV collagen”Ser. No. 377,238, filed on Jul.7, 1989, now U.S. Pat. No. 5,144,840 and the continuation-in-part application “Immunological methods for the detection of the human type IV collagen α5 chain”, filed Dec. 20, 1990, U.S. application Ser. No. 630,563 whereas the well characterized α1(IV) and α2(IV) chain are believed to be ubiquitous basement membrane (BM) components present in all BMs.

Using cDNA probes and both somatic cell-hybrids and in situ hybridization, the gene for the human type IV collagen α5 chain COL4A5 was localized to the q22 region on the long arm of chromosome X (Hostikka et al., Proc. Natl. Acad. Sci. USA. 87: 1606-1610, 1990 and the parent U.S. patent application “A Method for determining the nucleotide sequence of a novel α5(IV) chain of human type IV collagen” serial number 377,238, filed on Jul. 7, 1989, U.S. Pat. No. 5,144,840. This is different from the human genes COL4A1 and COL4A2 coding for the α1(IV) and α2(IV) chains that both are located on the terminal end of the long arm of the chromosome 13 (Boyd et al., Hum. Genet., 74: 121-125, 1986; Griffin et al., Proc. NatI. Acad. Sci. USA., 84: 512-516, 1987). The α1(IV) and α2(IV) chains are transcribed by different DNA strands from a common bidirectional promoter in opposite directions, so that the transcription initiation sites are separated by only 42-127 bp (Pöschl et al., EMBO J., 7: 2687-2695, 1988; Soininen et al., J. Biol. Chem., 263: 17217-17220, 1988).

The applicants have determined the complete structure of the human α1(IV) gene (Soininen et al., J. Biol. Chem.,264: 13565-13571, 1989) and the partial structure for the human α2(IV) gene (Hostikka and Tryggvason, FEBS Lett., 224: 297-305). The α1(IV) gene contains 52 exons spread over at least 100 kb of genomic DNA. The sizes of translated exons vary from 27 to 213 bp. The collagenous domain is encoded by 47 exons with sizes varying between 27 and 192 bp. About half of them begin with complete codons whereas the other half of the gene has mainly split codons, usually beginning with the second base for glycine, but also two exons begin with the third base of a codon. Thus, the exon size pattern of this gene is very different from the highly conserved structure of the genes for the fibrillar collagens. The largest exon coding for a translated sequence is the junction exon coding for the carboxyl-terminal part of the collagenous domain and the amino-terminal part of the NC domain. Four more exons code for the NC domain, the last of them containing the 3 untranslated region (Soininen et al., J. Biol. Chem., 264: 13565-13571, 1989).

The region characterized from the human α2(IV) gene shows a different pattern. The NC domain is encoded by three exons as compared to five in the α1(IV) gene. The similarity of the two genes is demonstrated by the fact that although the exon sizes differ, the locations of the introns are exactly the same when comparing to the aligned amino acid sequences of the chains. On the other hand, the exons in the collagenous domain coding region of the α2(IV) gene are different so that only one intron location seems to coincide, whereas all the exon sizes differ nor do they obey the fibrillar 54 bp pattern.

The exons in the collagenous region coding part begin with split glycine codons (Hostikka and Tryggvason, FEBS Lett., 224: 297-305).

Due to the wide distribution of basement membranes in the body, they are frequently affected in local and systemic diseases, and in many instances the consequent pathological changes lead to severe clinical complications. These diseases may be acquired i.e. complications of a disease that do not primarily involve basement membrane, or they can be genetically determined inherited diseases that are due to gene mutations leading to abnormal structure and function of the basement membrane. The best known example of an acquired disease is diabetes mellitus where the basement membrane structure is affected in almost all tissues in the body, resulting in dysfunction of small blood vessels (microangiopathy), kidneys (nephropathy), and nerves (neuropathy). The biochemical alterations leading to these malfunctions are still poorly understood. Also, autoimmune diseases, such as Goodpasture syndrome, affect the basement membranes. The antibody binds to the glomerular basement membrane and triggers its destruction by complement binding and phagocytosis.

Examples of inherited diseases are: (1) the congenital nephrotic syndrome that is characterized by extensive leakage of blood proteins through the renal glomerular basement membrane into the urine (proteinuria); and (2) the Alport syndrome where malfunction of the glomerular basement membrane leads to the passage of blood cells into urine (hematuria), eye lesions and hearing loss. The actual gene defect leading to the congenital nephrotic syndrome is yet completely unknown. The Alport syndrome is primarily an X-linked inherited kidney disease that has been linked by chromosomal markers to chromosome X region q22-26 (Atkin et al., Am. J. Hum. Genet., 42: 249-255, 188; Flinter et al., Genomics 4: 335-338, 1989) It leads to malfunction of kidneys and it can be treated only by dialysis or renal transplantation. The disease has been shown to be associated with progressive ultrastructural abnormalities, such as patchy thickening and thinning of the glomerular basement membrane and splitting of the lamina densa. These results and the more recent immunological studies have suggested that the cause of the Alport syndrome would be an abnormal or absent type IV collagen α chain (Spear, Clin. Nephrol. 1: 336-337, 1973; Kashtan et al., J. Clin. Invest., 78: 1035-1044, 1986).

Of importance with respect to the present invention is our recent discovery that a mutation in the type IV collagen α5(IV) gene, that changes the structure of the produced polypeptide chain, causes Alport syndrome. Eighteen Alport kindreds were studied and in three of them an abnormal fragment pattern was shown with restriction fragment length polymorphism (RFLP) analysis for the α5(IV) gene (Barker et al., Science 248: 1224-1227, 1990; and the U.S. patent application “Method for detection of Alport syndrome”, Ser. No. 07/534,786, filed on Jun. 7, 1990 now abandoned, and the parent U.S. patent application “Method for determining the nucleotide sequence of a novel α5(IV) chain of human type IV collagen” Ser. No.377,238, filed on Jul.7, 1989, now U.S. Pat. No. 5,144,840. In kindred EP, there was a deletion of about 15 kb of the gene, containing exons 5 through 10 as counted from the 3′ end. In kindred P, there was a point mutation that changed a codon for a conserved cysteine residue to a codon for serine and created restriction sites for Psil and Bgll restriction endonucleases (Barker et al., Science 248: 1224-1227, 1990; the U.S. patent application “Method for detection of Alport syndrome”, Ser. No. 07/534,786, filed on Jun. 7, 1990 now abandoned, and the parent U.S. patent application “A Method for determining the nucleotide sequence of a novel α5(IV) chain of human type IV collagen” Ser. No. 377,238, filed on Jul.7, 1989 now U.S. Pat. No. 5,144,840 and Zhou, et al., 1991a, Genomics, in press). Later studies have shown that in about 10 % of the Alport patients, a gene rearrangement can be observed with the α5(IV) cDNA clones in RFLP analysis.

The present invention provides for a method for isolation and partially characterizing the nucleotide sequence of the gene coding for the human type IV collagen α5(IV) chain. The invention provides for the use of the identified nucleotide sequence (or DNA fragments thereof) to detect mutation(s) in Individual genes specific for the α5(IV) chain which can, directly or indirectly, produce human diseases. The invention relates to the use of noncoding intervening sequences (introns) between and flanking the α5(IV) polypeptide chain coding regions (exons) of the α5(IV) gene to amplify and determine the physical properties and nucleotide sequence of the said gene for both protein coding regions and noncoding regions. Also, the Invention relates to the use of gene fragments generated through amplification from human genomic or cloned DNA for the detection and analysis of the gene, such as in detection of mutations. Additionally, the invention provides for the use of the Identified recombinant DNA cloning vectors and transformed hosts which contain a vector which has a fragment of the α5(iV) gene inserted into.

The invention further related to the detection of variations of an individual's COL4A5 gene in comparison with the known normal COL4A5 gene. In one embodyment this is done by RFLP analysis, wherein an individual's genomic DNA is cut by restriction enzyme, size-fractionated and tested for the sizes of DNA fragments of the α5(IV) gene. The invention relates to nucleotide sequences flanking the polypeptide chain coding region (intron sequences) that can be used to amplify coding regions (exons) with the flanking consensus sequences needed for the proper splicing of the pre-mRNA to mRNA by cloning or the method of polymerase chain reaction (PCR) or any other method known to the art; and to determining the differences of those sequences with the normal COL4A5 gene by various techniques, such as single-strand conformation polymorphism analysis, denaturing gradient gel electrophoresis, S1 nuclease mapping nucleotide sequencing or any other method known to the art.

Finally, the invention related to the fragments of the normal human α5(IV) gene that may by used to correct a defective gene by gene therapy.

The following is a description of the drawings which are presented for the purpose of illustrating the invention and not for the purpose of limiting same.

FIG. 1 is a restriction map of eight cloned fragments of the α5(iV) gene. A scale demonstrating the size differences in kilo base pairs is shown below the cloned gene fragments (in the middle). The EcoRI restriction sites (E) and the aligned positions of the eigth λ clones are shown. On top is a diagram of the gene with α5(IV) polypeptide chain coding regions (exons) indicated by shadowed boxes and noncoding regions (introns and the 3′ flanking region) indicated by a solid line. The exons are numbered from the 3′end of the gene.

FIG. 2 shows the nucleotide sequence of the 19 most 3′ exons of the α5(IV) gene and flanking regions. Exon sequences (SEQ ID NO: 1 through SEQ ID NO: 19 ) are shown in capital letters and Intron sequences in lower case letters. The exons are numbered from the 3′ end.

FIG. 3 is a table where the sizes of the exons and introns of the α5(IV) gene Is compared with those of the α1(IV) gene. The exon sizes differ only once, in exon 19 (SEQ ID NO: 19) where one amino acid residue is missing from the α5(IV) chain when comparing the aligned amino acid sequences with those of the α1(IV) chain. On the other hand, the introns show no similarity in their size pattern.

FIG. 4 shows partial sequence of a PCR-amplified segment of the α5(IV) gene from normal individuals and individuals carrying a mutated gene. The amplified fragments were cloned into an M13 vector and sequenced with the dideoxynucleotide chain termination method. The sequencing reaction samples were electrophoresed on a polyacrylamide gel followed by autoradiography. The results show sequence differences between normal and mutated DNA.

The applicants have isolated and characterized fragments of the gene for the human type IV collagen α5(IV) chain and determined the structure and partial sequence (SEQ ID NO: 1-SEQ ID NO: 19) for the gene. The gene clones in x phage were screened with the cDNA clones coding for the human α5(IV) chain (Hostikka et al., Proc. Natl. Acad. Sci. USA. 87: 1606-1610, 1990 and the parent U.S. patent application “A Method for determining the nucleotide sequence of a novel α5(IV) chain of human type IV collagen” serial number 377,238, filed on Jul. 7, 1989, now U.S. Pat. No. 5,114,840). The genomic clones obtained, cover approximately 50 kb or about half of the COL4A5 gene in eight overlapping clones (Zhou, et al., (1991), Genomics, Vol. 9(1) 10-18 Map of the cIones with respect to each other and the encoded exons with the restriction sites for EcoRl are shown in FIG. 1 .

Fragments of the genomic clones for the COL4A5 gene that hybridized with the cDNA clones and therefore contained coding regions, were subcloned Into M13 and/or pUC-vectors for sequencing and further restriction mapping. The coding nucleotide sequence of the cDNAs was used to make oligonucleolide primers for sequencing the exons and their flanking regions from the genomic clones; By the sequence identity of the α5(IV) chain with the α1 (IV) chain, it was anticipated that the exon-intron boundaries would coincide, as compared to the aligned amino acid sequences. This was shown to be the case, as shown in FIG. 3 . Six overlapping genomic clones (ML-5, MG-2, EB-4, FM-13, F-2 and F-7) cover about 9.5 kb of the 3flanking region and about 36 kb of the gene, including 14 most 3 exons. Two additional clones (F-8 and ML-2) that do not overlap the previous clones, contain exons 16 through 19(SEQ ID NO: 16-SEQ ID NO: 15) Exon 15 was shown to be one exon and sequenced by amplifying the coding region with PCR reaction, using cDNA derived primers. The exons 1-5(SEQ ID NO: 1-SEQ ID NO: 5) code for the NC domain. Exon 5(SEQ ID NO: 5) is a Junction exon that contains 142 bp coding for the NC domain and 71 bp coding for the collagenous region. Exons 6-19(SEQ ID NO: 6-SEQ ID NO: 19)code for collagenous Gly-X-Y-repeat sequence and have sizes differing between 51 and 186 bp. The exons and their flanking Intron sequences are shown In the FIG. 2 . The intron sizes analyzed very between 133 and 7000 bp and show no correlation with the sizes of the corresponding introns in the α1(IV) gene.

(1) GENERAL INFORMATION:

(iii) NUMBER OF SEQUENCES: 23

(2) INFORMATION FOR SEQ ID NO:1:

(A) LENGTH: 1731 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Not relevant

(iv) ANTI-SENSE: Not relevant

(v) FRAGMENT TYPE: Eco RI restriction

fragments

(A) ORGANISM: Human

(B) STRAIN: Not relevant

(C) INDIVIDUAL ISOLATE: Not relevant

(D) DEVELOPMENTAL STAGE: Not relevant

(E) HAPLOTYPE: Not relevant

(F) TISSUE TYPE: Liver

(G) CELL TYPE: Not relevant

(H) CELL LINE: Not relevant

(I) ORGANELLE: Not relevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic library in lambda

Charon 4A

(B) CLONE: M1-5, MG-2

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Not relevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exxon 1 region from 3′ end of gene

(C) IDENTIFICATION METHOD: DNA sequencing

(D) OTHER INFORMATION: The sequence contains 205

nucleotides from intron 1 (lower case letters),

1241 nucleotides of exon 1 (capital letters)

and 285 nucleotides of 3′ flanking region

(lower case letters)

(x) SEQUENCE DESCRIPTION: SEQ ID NO:1:

agaat cgttacaaaa tatctatgac 25

cactaatggc tcttaaacca tactagtcac tgcattagat catattgctg aaaagtaaac 85

cattaagtca ccaagagagc tacttaacac acaaaaaatg tttagttata aatttgaacc 145

agtaacagaa ttgaaatacc agaaaatgtg gatctgattg tcttatttct tatttcccag 205

TAAACCTCAG TCAGAAACGC TGAAAGCAGG AGACTTGAGG ACACGAATTA GCCGATGTCA 265

AGTGTGCATG AAGAGGACAT AACATTTTGA AGAATTCCTT TTGTGTTTTA AAATGTGATA 325

TATATATATA TAAAATTCCT AGGATGCAGT GTCTCATTGT CCCCAACTTT ACTACTGCTG 385

CCGTCAATGG TGCTACTATA TATGATCAAG ATAACATGCT GACTAGTAAC CATGAAGATT 445

CAGATGTACC TCAGCAATGC GCCAGAGCAA AGTCTCTATT ATTTTTCTAC TAAAGAAATA 505

AGGAAGTGAA TTTACTTTTT GGGTCCAGAA TGACTTTCTC CAAGAATTAT AAGATGAAAA 565

TTATATATTT TGCCCAGTTA CTAAAATGGT ACATTAAAAA TTCAATTAAG AGAAGAGTCA 625

CATTGAGTAA AATAAAAGAC TGCAGTTTGT GGGAAGAATT ATTTTTCACG GTGCTACTAA 685

TCCTGCTGTA TCCCGGGTTT TTAATATAAA GGTGTTAAGC TTATTTTGCT TTGTAAGTAA 745

AGAATGTGTA TATTGTGAAC AGCCTTTTAG CTCAAAATGT TGAGTCATTT ACATATGACA 805

TAGCATGAAT CACTCTTTAC AGAAAATGTA GGAAACCCTA GAATACAGAC AGCAATATTT 865

TATATTCATG TTTATCAAAG TGAGAGGACT TATATTCCTA CATCAAGTTA CTACTGAGAG 925

TAAATTTATT TTGAGTTTTA TCCCGTAAGT TCTGTTTTGA TTTTTTTTAA AAAACAAACC 985

CTTTTAGTCA CTTTAATCAG AATTTTAAAT GTTCATGTTA CATACCAAAT TATAATATCT 1045

AATGGAGCAA TTTGTCTTTT GCTATATTCT CCAAGATTAT CTCTTAAGAC CATATGCCCC 1105

CTGTTTTAAT GTTTCTTACA TCTTGTTTTT ACTCATTTCT GACTGGACAA AGTTCTTCCA 1165

AACAATTCTG AGAAACAAAA ACACACACGC AGAATTAACA ATTCTTTTCC CTGTGCTTCT 1225

TATGTAAGAA TCCTCCTGTG GCCTCTGCTT GTACAGAACT GGGAAACAAC ACTTGGTTAG 1285

TCTCTTTTAA GTTACAAAAA GCCAATTGAT GTTTCTTATT CTTTTTAAAT TTTAAATATT 1345

TTGTTATAAA TACTCACAGG ATACCTTATT TCCCTAGCTA TCATCTCCTG ACTTAATGTT 1405

TTTTAAACCC ACCAATATAA ATTTAATTAA AGATATATGT Tgtaaggatg gtctgttgtg 1465

tatctcttca gcctgtgtgg aaaaaacccc tgctcattta cagatagatt accaatctgc 1525

acatcaacaa gtcatctctt cccagggaat cagtttctcc acgctttgat cactctgtta 1585

gtagatagaa aacatatagt aatgtaaact ttttccaatg aagaaaacta cctattggaa 1645

gacatttcca agataataaa tttcttgaca acattgtgtt aatggctaag aaaggaaaca 1705

actggcttct atttggggaa gaattc 1731

(2) INFORMATION FOR SEQ ID NO:2:

(A) LENGTH: 503 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Liver

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic library in

Charon 4A

(B) CLONE: ML-5

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 2 region counted from

3′ end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence starts with 246

nucleotides of intron 2 (lower case letters),

followed by 173 nucleotides of exon 2 (capital

letters) and ends with 84 nucleotides from

downstream intron 1 (lower case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:2:

aagcttaaac ttcaaacagc ttctatccaa gcactgtgtt ccccctcaca cattttttgt 60

aacatatttt cataatctgc ctattacttt tttttgacta cccttggtgt ggatactatt 120

gtcttacctc tgggcctgtt ccttcactag atttgaattt ggccaagctc agcacacatc 180

tttggatatt cactaagcta ttatggcaca tgggtattgc ggcacatttt tccttgtctt 240

ttatagCATA CAAGTGCAGG GGCAGAAGGC TCAGGTCAAG CCCTAGCCTC CCCTGGTTCC 300

TGCTTGGAAG AGTTTCGTTC AGCTCCCTTC ATCGAATGTC ATGGGAGGGG TACCTGTAAC 360

TACTATGCCA ACTCCTACAG CTTTTGGCTG GCAACTGTAG ATGTGTCAGA CATGTTCAGg 420

taaagtgctt atagctttaa ttcaggtcca aagcttcctt cagagatgct agggaagaaa 480

gagacaattt atggatggtt tat 503

(2) INFORMATION FOR SEQ ID NO:3:

(A) LENGTH: 360 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Liver

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in Charon

4A vector

(B) CLONE: ML-5

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 3 region counted

from 3′ end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 141

nucleotides of intron 3 (lower case letters), 115

nucleotides of exon 3 (capital letters) and 104

nucleotides from downstream intron 2 (lower case

letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:3:

aagtgcattt tttcaccttt tgtgatcatt gaaagagaca ttaatcggct tccatactaa 60

gaaggcttcc aatgaagcag gatggctact tctcacatgc tcactctgta gattatgttc 120

cttctccttt tcctttacca gATGTGCAGT ATGTGAAGCT CCAGCTGTGG TGATCGCAGT 180

TCACAGTCAG ACGATCCAGA TTCCCCATTG TCCTCAGGGA TGGGATTCTC TGTGGATTGG 240

TTATTCCTTC ATGATGgtat tttacactct tccttgcatt tgtcatcata gctgactgtc 300

cattccatct acatttcttc ccaatatgag gaatccctgt catttgcata ataagaagct 360

(2) INFORMATION FOR SEQ ID NO:4:

(A) LENGTH: 433 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Liver

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in Charon 4

A vector

(B) CLONE: ML-5

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 4 region counted

from 3′ end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 138

nucleotides of intron 4 (lower case letters), 178

nucleotides of exon 4 (capital letters) and 117

nucleotides from downstream intron 3 (lower case

letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:4:

aactaaggaa atatagtata tttattacct atgaaaggct ggcaagtttc cttgaaaggc 60

tgtttgctat tgttttagaa gaaaccaaag tatcattatc acgcagtcct ttactgtttt 120

ctctccaaat ctttctagGG ACGGCTGGCA GCTGCCTTCG TCGCTTTAGT ACCATGCCTT 180

TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAC TATTCTTACT 240

GGCTCTCTAC CCCAGAGCCC ATGCCAATGA GCATGCAACC CCTAAAGGGC CAGAGCATCC 300

AGCCATTCAT TAGTCGgtaa ggcattgatt tagctgtgac ttttaccaat ccccagttag 360

ttagctagtc agatttgagt ccaaagctaa caatctgatg atattcctcc taggttaggt 420

agaacagagg tac 433

(2) INFORMATION FOR SEQ ID NO:5:

(A) LENGTH: 461 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

FIX and EMBL vectors

(B) CLONE: FM-13 and EB-4

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 5 region counted

from 3′ end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 159

nucleotides of intron 5 (lower case letters), 213

nucleotides of exon 5 (capital letters) and 89

nucleotides from downstream intron 4 (lower case

letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:5:

tttgtctcct agcccatgat atctgacaat gcctttatag acactctata aatatcaaat 60

aaattaacta gtcaaatgag gtcataatgt tttgtcaata tccataagag tggatcagag 120

cttacttaat cttgtatact gattatttcg tggaaatagG TACCCGTGGT TTGGATGGTC 180

CCCCTGGTCC AGATGGATTG CAAGGTCCCC CAGGTCCCCC TGGAACCTCC TCTGTTGCAC 240

ATGGATTTCT TATTACACGC CACAGCCAGA CAACGGATGC ACCACAATGC CCACAGGGAA 300

CACTTCAGGT CTATGAAGGC TTTTCTCTCC TGTATGTACA AGGAAATAAA AGAGCCCACG 360

GTCAAGACTT GGgtgagata atcaatatct aatttcctac tgtgcctttt gtttttgttt 420

cagaaatcct gttgggttct agagtagcct tggccaaagt g 461

(2) INFORMATION FOR SEQ ID NO:6:

(A) LENGTH: 308 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

Fix vector

(B) CLONE: FM-13

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 6 region counted

from 3′ end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 78

nucleotides from intron 6 (lower case letters),

99 nucleotides of exon 6 (capital letters) and

131 nucleotides from downstream intron 5 (lower

case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:6:

agaaccttat gtctcctaga tctgtccaga tgtgaaaata ttctactcat atttgaatgc 60

ctcattcttt tcctgtagGT CCAACTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC 120

CTGGCTTTGA TGGTGCAGGA GGGCGCAAAG GAGACCCAGG TCTGCCAGGA CAGCCAGgta 180

agacaagtaa aacatgctgt tggtggaggg aaagtctttg agtctgagag acctctggac 240

atagggcctc cacttagagc tgtgagatct tctcttgatg ttagcatagc tgactggtga 300

atgtggac 308

(2) INFORMATION FOR SEQ ID NO:7:

(A) LENGTH: 280 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

Fix vector

(B) CLONE: FM-13 and F-2

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 7 region counted

from 3′ end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 62

nucleotides from intron 7 (lower case letters),

129 nucleotides of exon 7 (capital letters) and

89 nucleotides from downstream intron 6 (lower

case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:7:

tttggcttcc atttcttgta acctttctct ttcccttcaa atttgtgtgt tttgtctcat 60

agGTCCTCCT GGATTACCTG GTCCTTCAGG ACAGAGTATC ATAATTAAAG GAGATGCTGG 120

TCCTCCAGGA ATCCCTGGCC AGCCTGGGCT AAAGGGTCTA CCAGGACCCC AAGGACCTCA 180

AGGCTTACCA Ggtaccaatg cagatcatct ttattatcat tattatactt ttaatttctg 240

ggatacatgt gcagaatgta caggtttgtt acataggtat 280

(2) INFORMATION FOR SEQ ID NO:8:

(A) LENGTH: 206 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

Fix vector

(B) CLONE: FM-13 and F-2

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon region 8 counted

from 3′ end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 33

nucleotides of intron 8 (lower case letters), the

72 nucleotides exon 8 (capital letters) and 101

nucleotides of intron 7 (lower case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:8:

tttgc cataaaactg tatgtacctt ctgtgcagGC ATGAAAGGAC CCAGTGGAGT 55

ACCTGGATCA GCTGGCCCTG AGGGGGAACC GGGACTTATT GGTCCTCCAG gtaagactta 115

ttcctgaaga tagttatacc tgatacttag atgctttaaa gaatttgaaa gttttcattc 175

tgtctttcag ccagaccatc ggaggctaag t 206

(2) INFORMATION FOR SEQ ID NO:9:

(A) LENGTH: 365 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

Fix vector

(B) CLONE: FM-13 and F-2

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon region 9 counted

from 3′ end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 192

nucleotides of intron 9 (lower case letters),

followed by 73 nucleotides exon 9 (capital

letters), and 100 nucleotides intron 8 (lower

case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:9:

aaactatgaa tcaaggaggt taaataatca actcaattca cacaagataa tataaggcaa 60

aattgagatt acagtcttgg aagtttgact ctagaaatag tgctatatgc cactatgtaa 120

ttcttatgcc ctcaatcacc ttcctcccct cgctgcaatt tttttgtaac attaatgatt 180

ttatttattc agGGTAATCC TGGCCGGCCG GGTCTCAATG GAATGAAAGG AGATCCTGGT 240

CTCCCTGGTG TTCCAGGATT CCCAGgtatt tgaagggatt tttgtggttt ccctttatat 300

taaactcctc tgggacaaga tagccatttt ctgatttgac tgggtaaagg ttgtagccct 360

gttgc 365

(2) INFORMATION FOR SEQ ID NO:10:

(A) LENGTH: 286 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

Fix vector

(B) CLONE: F-7

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 10 region from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 86

nucleotides of intron 10 (lower case letters),

134 nucleotides exon 10 (capital letters) and 66

nucleotides of downstream intron 9 (lower case

letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:10:

gaagatgact gatattttaa aagcctgact tttatgctac tcttaacact atactgaaat 60

gtcgtcattt gctgtggatt attaagGTCT ACCAGGTCCA GAAGGTCCTC CAGGTCTCCC 120

TGGAAATGGA GGTATTAAAG GAGAGAAGGG AAATCCAGGC CAACCTGGGC TACCTGGCTT 180

GCCTGGTTTG AAAGGAGATC AAGGACCACC AGGACTCCAG gtaggaaatg gaagtagata 240

tctgatgaga gaagaatgtg ggtgtttgta ttcaaaatgt gaattc 286

(2) INFORMATION FOR SEQ ID NO:11:

(A) LENGTH: 507 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

Fix vector

(B) CLONE: F-7

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 11 region from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 94

nucleotides of intron 11 (lower case letters),

186 nucleotides of exon 11 (capital letters) and

227 nucleotides of downstream intron 10 (lower

case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:11:

ccattaattg ccctaatgta tgtgaatagc taaccttata agcaagcttg taactcggta 60

ttatttatct tctaattata ctttactttc atagGCCAAA AGGGTGATGG AGGATTACCT 120

GGGATTCCAG GAAATCCTGG CCTTCCAGGT CCAAAGGGCG AACCAGGCTT TCACGGTTTC 180

CCTGGTGTGC AGGGTCCCCC AGGCCCTCCT GGTTCTCCGG GTCCAGCTCT GGAAGGACCT 240

AAAGGCAACC CTGGGCCCCA AGGTCCTCCT GGGAGACCAG gtatgtccgt gagtggtagg 300

agaatggtct atttattagt ccatgtattt cgttttgctg gcaggttatt cagtctttaa 360

gactttagaa tttttccggt gcattggagg atgttaaaaa aaagacttta aaatttgtga 420

tataacttct tacaagtaaa tagcttggtt catagctaac tccatctatt tccatggttc 480

taataatttg ctttagtaat gcatttt 507

(2) INFORMATION FOR SEQ ID NO:12:

(A) LENGTH: 191 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: genomic in lambda Fix

vector

(B) CLONE: F-7

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 12 region from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 53

nucleotides from intron 12 (lower case letters),

51 nucleotides exon 12 (capital letters) and 87

nucleotides of downstream intron 11 (lower case

letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:12:

cagttgtatt atccacttga gtttttgttt tgttttgttt tgtactctga cagGTCAACC 60

AGGCTTTGGA AACCCAGGAC CCCCTGGACT TCCAGGACTT TCTGgtaaac cttaataaaa 120

catgctaaat caatctataa taaaatgaga ttatttccaa atacatctat ttttccatct 180

ccacctttta c 191

(2) INFORMATION FOR SEQ ID NO:13:

(A) LENGTH: 349 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: genomic library in lambda

Fix vector

(B) CLONE: F-7

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 13 region from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 181

nucleotides from intron 13 (lower case letters),

99 nucleotides exon 13 (capital letters) and 69

nucleotides of downstream intron 12 (lower case

letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:13:

agatgctgaa tgactattcc ttattttcat tatcctcctt catattttta taacattttg 60

tgatccaaag gagtgtctca aaagcacctt gtttcttttg gataaagaag ggagcatatg 120

gaagtaaaag ggagttggaa attggaaaac tgggtgtaac ctgctgtact caatttttta 180

gGTGGTGGAG GTCATCCTGG GCAACCAGGG CCTCCAGGCG AAAAAGGCAA ACCCGGTCAA 240

GATGGTATTC CTGGACCAGC TGGACAGAAG GGTGAACCAG gtgctgtagt ttttcatttt 300

tcctattttt ctaattttct ctgtgttgaa tttaacttgc ctttttatt 349

(2) INFORMATION FOR SEQ ID NO:14:

(A) LENGTH: 292 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Irrelevant

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

Fix vector

(B) CLONE: F-7

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 13 region from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: Sequence contains 113

nucleotides from intron 14 (lower case letters),

81 nucleotides exon 14 (capital letters) and 98

nucleotides of downstream sequence (intron 13,

lower case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:14:

ttctttttgt tcttcactgt ttctatgcta gcactataaa ttgtaagttt gaattgtagc 60

tcttaaagca atgcagtttt tctttcattt ttaaattgag ctctttactc tagGAACCCC 120

AGGCCCTCCT GGACCAAAAG GTATTAGTGG CCCTCCTGGG AACCCCGGCC TTCCAGGAGA 180

ACCTGGTCCT GTAGgtaagc atgaaaaata acagtttgct gttttataaa actaatgttt 240

atcatattaa gtttgggaaa gtcaaatcat gttcagctgt gaacattttc aa 292

(2) INFORMATION FOR SEQ ID NO:15:

(A) LENGTH: 127 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: PCR amplified fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Leukocyte

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: None

(B) CLONE: PCR amplified exon 15

cloned in M13 vector

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 15 counted from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: The sequence contains the

127 nucleotides exon 15.

(x) SEQUENCE DESCRIPTION: SEQ ID NO:15:

GGTGAGCCTG GTCTGCCTGG ATACCCAGGG AACCCTGGTA TCAAAGGTTC TGTGGGAGAT 60

CCTGGTTTGC CCGGATTACC AGGAACCCCT GGAGCAAAAG GACAACCAGG CCTTCCTGGA 120

TTCCCAG 127

(2) INFORMATION FOR SEQ ID NO:16:

(A) LENGTH: 264 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

FIX vector

(B) CLONE: F8

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 16 region from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: The sequence contains 100

nucleotides from intron 16 (lower case letters),

140 nucleotides exon 16 (capital letters) and 24

nucleotides of intron 15 (lower case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:16:

ggagcttttt aaaaatcttt ttgctttgtc atatgcatct tagataatcc acaagtaaag 60

catattttgt aaaatattat atatcacata ttttcaacag GGCCTCAGGG TGTGGAAGGG 120

CCTCCTGGAC CTTCTGGAGT TCCTGGACAA CCTGGCTCCC CAGGATTACC TGGACAGAAA 180

GGCGACAAAG GTGATCCTGG TATTTCAAGC ATTGGTCTTC CAGGTCTTCC TGGTCCAAAG 240

gtaatctttg gcatatagtt ttag 264

(2) INFORMATION FOR SEQ ID NO:17:

(A) LENGTH: 330 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Irrelevant

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

FIX vector

(B) CLONE: F8

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 17 region from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: The sequence contains 84

nucleotides of intron 18 (lower case letters), 90

nucleotides of exon 17 (capital letters) and 156

nucleotides of intron 17 (lower case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:17:

attttaatga ctatccattc ccatgaaacc agacaacccc aatattgcta cattgtctta 60

attttaccaa tttgaccttt ctagGTCCCA AAGGTAACCC TGGTCTCCCT GGACAGCCAG 120

GTCTTATAGG ACCTCCTGGA CTTAAAGGAA CCATCGGTGA TATGGGTTTT CCAGgtgagt 180

gatgaaaatc ttccaaatat ttagtcccat taatgaaagg tggttcaata tctctttttt 240

tgtcagaaaa gaggctggtg ttgatagaat cagactgaaa cgatatctga ggtaatcagt 300

gggtagtgtt ctcttgttac acaaatattt 330

(2) INFORMATION FOR SEQ ID NO:18:

(A) LENGTH: 307 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Lung

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic in lambda

FIX vector

(B) CLONE: F8

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 18 region from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: The sequence contains 159

nucleotides from intron 18 (lower case letters),

99 nucleotides of exon 18 (capital letters) and

49 nucleotides of intron 17 (lower case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:18:

tttcgtgtga gtccagtgct aatagctcat actatatcag aatatcacca gttcctctaa 60

ttcacttata gtttaacact tgagtagctt gctttgccaa agttatttca tggatgaata 120

atatcatcct aacttgcctc ttctactcat tcttggaagG TATACCTGGA GTTTCAGGGC 180

CAAAAGGTTA TCAGGGTTTG CCTGGAGACC CAGGGCAACC TGGACTGAGT GGACAACCTG 240

GATTACCAGG ACCACCAGgt aagtgtgata ggccatttgt agcaattgct tagctgacac 300

tgaattc 307

(2) INFORMATION FOR SEQ ID NO:19:

(A) LENGTH: 304 base pairs

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(v) FRAGMENT TYPE: Endonuclease restriction

fragment

(A) ORGANISM: Human

(B) STRAIN: Irrelevant

(C) INDIVIDUAL ISOLATE: Irrelevant

(D) DEVELOPMENTAL STAGE: Irrelevant

(E) HAPLOTYPE: Irrelevant

(F) TISSUE TYPE: Liver

(G) CELL TYPE: Irrelevant

(H) CELL LINE: Irrelevant

(I) ORGANELLE: Irrelevant

(vii) IMMEDIATE SOURCE:

(A) LIBRARY: Human genomic library

(lambda FIX, Stratagene, CA)

(B) CLONE: F8

(viii) POSITION IN GENOME:

(B) MAP POSITION: q22

(C) UNITS: Irrelevant

(ix) FEATURE:

(A) NAME/KEY: COL4A5 collagen gene

(B) LOCATION: Exon 19 region from 3′

end of gene

(C) IDENTIFICATION METHOD:DNA sequencing

(D) OTHER INFORMATION: The sequence contains 71

nucleotides from intron 19 (lower case letters),

150 nucleotides of exon 19 (capital letters) and

83 nucleotides of intron 18 (lower case letters).

(x) SEQUENCE DESCRIPTION: SEQ ID NO:19:

ataatcttta tatgcattaa tctttgatgg ataaaattga tatattgtgt tttcacacac 60

attgatttta gGTGATGATG GCTTGCAGGG TCAGCCAGGA CTTCCTGGCC CTACAGGAGA 120

AAAAGGTAGT AAAGGAGAGC CTGGCCTTCC AGGCCCTCCTG GACCAATGGA TCCAAATCT 180

TCTGGGCTCA AAAGGAGAGA AGGGGGAACC TGGCTTACCAG gtgagtgaat gaatttatt 240

tatgaatatt tttcctgata tatctgaagt ttaatttttaa atagcatgaa aagtgactt 300

ataa 304

(2) INFORMATION FOR SEQ ID NO:20:

(A) LENGTH: 28

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(x) SEQUENCE DESCRIPTION: SEQ ID NO:20:

CTAGAATTCG GTGAGCCTTG GTCTGCCT 28

(2) INFORMATION FOR SEQ ID NO:21:

(A) LENGTH: 27

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(x) SEQUENCE DESCRIPTION: SEQ ID NO:21:

CCGAAGCTTC TGGGAATCCA GGAAGGC 27

(2) INFORMATION FOR SEQ ID NO:22:

(A) LENGTH: 27

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(x) SEQUENCE DESCRIPTION: SEQ ID NO:22:

GACTCTAGAA AGGCCATTGC ACTGGTT 27

(2) INFORMATION FOR SEQ ID NO:23:

(A) LENGTH: 27

(B) TYPE: Nucleic acid

(C) STRANDEDNESS: Single

(D) TOPOLOGY: Linear

(ii) MOLECULE TYPE: Nucleotide-genomic DNA

(iii) HYPOTHETICAL: Irrelevant

(iv) ANTI-SENSE: Irrelevant

(x) SEQUENCE DESCRIPTION: SEQ ID NO:23:

AGCGAATTCC TGACCTGAGT CATGTAT 27