The present disclosure relates to the field of medicinal chemistry and particularly to the development of heterocyclic compounds and a process of preparation of the same. The present disclosure more particularly to the pyrazolopyrimidine compounds and in particular diaminopyrazolopyrimidine compounds which act as antagonists for adenosine receptors.

›BACKGROUND

Adenosine is a purine nucleoside produced by cells for its intracellular physiological and extracellular signaling processes. It is synthesized from inosine monophosphate (IMP) as a nucleotide from adenosine monophosphate (AMP) or by the hydrolysis of adenosine triphosphate (ATP) via adenosine diphosphate (ADP) and AMP. Apart from being one of the building blocks of DNA, it is the primary energy source of cells as adenosine triphosphate (ATP) and is involved in the all-pervasive signal transduction processes as cyclic adenosine monophosphate (cAMP). Extracellular adenosine regulates inflammation, allostasis and cognitive functions in the brain, and vasodilation in the heart, lungs, and kidneys, all brought about by its binding to the adenosine receptors.

Adenosine receptors are members of the guanine nucleotide protein-coupled receptors (GPCR) family on the cell surface and consist of A 1 R, A 2a R, A 2b R, and A 3 R subtypes in humans (Jacobson et al., 2012, PMID: 22371149). A 1 and A 3 receptor couple to Ga i/o , in contrast, A 2a and A 2b receptors couple to Ga s to inhibit or stimulate adenylate cyclase (AC) respectively and are distributed ubiquitously throughout the body (Borea et al., 2018, DOI: 10.1007/978-3-319-90808-3; Cheng et al., 2017, PMID: 28712806). Their agonist or antagonist ligands are used as pharmacological interventions of various pro- and anti-inflammatory mediators to manage asthma, chronic pulmonary obstructive disease, heart failure, arrhythmia, diabetic kidney disease, glaucoma, stroke, sleep, anxiety, neurodegenerative disorders like Parkinson's, Alzheimer's and Huntington's diseases, epilepsy, pain, cognition and memory, and cancer (Sek et al., 2018, PMID: 30513816).

During infection, injury, hypoxia, ischemia, or seizure, extracellular adenosine is upregulated in tissues as an allostatic measure to protect tissue damage from the inflammatory responses. Similar immunosuppressive mechanisms of elevated adenosine levels (in the tumor micro-environment, TME) have been identified in tumor immune evasion. In the TME, adenosine binds to A 2a R and A 2b R on various immune cells and suppresses them by activating the cAMP-dependent Protein Kinase A (PKA) and blockade of the nuclear factor-kB (NF-kB) and Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway (Allard et al., 2020, PMID: 32514148). Relieving this inhibition of the A 2a R and A 2b R by selective antagonists can make the immune cells capable of killing tumor cells (Vijayan et al., 2017; PMID: 29162946, Allard et al., 2020, PMID: 32514148). Thus, in the present scenario of increasing proliferative disorders, or diseases or conditions, there is a huge need for antagonists of adenosine receptors. Hence there is a huge need for the antagonists of adenosine receptors, which will be useful in treating condition related to cancer or immune-related disorders mediated by these receptors.

›SUMMARY OF THE INVENTION · 1 of 2

In an aspect of the present disclosure, there is provided a compound of Formula I

or its pharmaceutically acceptable salts, complexes, hydrates, solvates, tautomers, polymorphs, stereoisomers, racemates, pharmaceutically active derivatives thereof,

wherein A is selected from C 5-10 aryl, C 2-10 heteroaryl, C 3-6 cycloalkyl, or C 1-10 heterocyclyl; wherein C 5-10 aryl, C 2-10 heteroaryl, C 3-6 cycloalkyl, or C 1-10 heterocyclyl is optionally substituted with one or more substituents selected from R 6 , R 7 , R 8 , R 9 , R 9a , or oxo;

R 4 and R 5 are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, —CO—N(R 10 R 11 ), C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 2-6 alkenyl or C 2-6 alkynyl are optionally substituted with one or more groups selected from C 3-6 cycloalkyl, C 1-6 alkoxy, or C 1-10 heterocyclyl; or R 4 and R 5 are joined together to form C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 4-10 carbocyclyl or C 1-10 heterocyclyl is optionally substituted with 1 to 3 groups independently selected from halogen, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy;

R 10 and R 11 are independently selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 3-6 halocycloalkyl, C 4-10 carbocyclyl, or C 1-10 heterocyclyl;

R 6 is selected from hydrogen, halogen, hydroxyl, C 1-6 alkyl, C 1-6 haloalkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 alkoxy, C 1-6 haloalkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 3-6 cycloalkyl, C 3-6 halocycloalkyl, —Y—O—C 1-6 alkyl, —Y—O—C 3-6 cycloalkyl, —Y—CO—NH—R 13 , —Y—Z, C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 1-6 alkoxy, C 3-6 cycloalkyl, C 1-6 haloalkyl, C 1-6 aminoalkyl, C 1-6 alkylhydroxy, or C 1-10 heterocyclyl is optionally substituted with one or more groups selected from halogen, hydroxyl, amine, C 1-6 alkoxy, C 3-6 cycloalkyl, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 1-6 haloalkoxy, C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 3-6 cycloalkyl, or C 1-6 aminoalkyl, is optionally further substituted with one or more groups independently selected from halogen, hydroxyl, C 1-6 alkylhydroxy, —C(O)C 1-6 alkyl, —C(O)NH 2 , —C(O)—C 1-6 alkylhydroxy, or C 1-10 heterocyclyl, and C 1-10 heterocyclyl has 1 to 4 heteroatoms independently selected from O, N or S, and optionally substituted with 1 to 3 groups independently selected from halogen, hydroxyl, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy;

Y is selected from C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-6 cycloalkyl, or C 3-6 cycloalkenyl; and C 1-6 alkyl is optionally substituted with C 3-6 cycloalkyl;

Z is selected from —OH, —NH 2 , —COOH, —SO 2 NH 2 , —SO 2 CH 3 , —SOCH 3 , —SCH 3 , CH 2 —NH—(C 1-6 alkyl)-SOCH 3 , —O—P(═O)(OH) 2 , —CONH 2 , —CONH(C 1-6 alkyl), —SO 2 NH(C 1-6 alkyl), CON(C 1-6 alkyl) 2 , —NHCO(C 1-6 alkyl), C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 1-10 heterocyclyl has 1 to 4 heteroatoms independently selected from O, N or S, and optionally substituted with 1 to 3 groups independently selected from halogen, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy;

R 13 is selected from hydrogen, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl;

R 7 , R 8 , R 9 , and R 9a are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl;

and

n is 0, 1, or 2.

In another aspect of the present disclosure, there is provided a process of preparation of compounds of Formula I or its pharmaceutically acceptable salts, complexes, hydrates, solvates, tautomers, polymorphs, stereoisomers, racemates, pharmaceutically active derivatives thereof, said process comprising: reacting Formula (A), and Formula (B) in the presence of a base to obtain the compounds of Formula I,

wherein R is selected from C 1-6 alkyl, C 3-6 cycloalkyl, C 5-10 aryl, C 2-10 heteroaryl, or C 1-10 heterocyclyl; and the substituents are as defined above.

In one another aspect of the present disclosure, there is provided a pharmaceutical composition comprising a compound of Formula I as disclosed herein and one or more additional therapeutic agents.

In yet another aspect of the present disclosure, there is provided a method of treatment and/or prevention of a condition mediated by adenosine receptor or a proliferative disorder or cancer, comprising administering to a subject suffering from a condition mediated by adenosine receptor or a disease or proliferative disorder or cancer, a therapeutically effective amount of the compounds of Formula I as disclosed herein or the pharmaceutical composition comprising compounds of Formula I as disclosed herein and one or more additional therapeutic agent.

In further another aspect of the present disclosure, there is provided use of the compounds as disclosed herein or the pharmaceutical composition as disclosed herein for treatment of a condition mediated by adenosine receptor A 2a R, or A 2b R; treatment and/or prevention of a proliferative disorder or disease or cancer or immune-related disorder or disease or condition; or treatment of cancer together with other clinically relevant cytotoxic agents or non-cytotoxic agents.

In furthermore aspect of the present disclosure, there is provided use of the compounds as disclosed herein or the pharmaceutical composition as disclosed herein for treatment of a condition mediated by adenosine receptors A 2a R and A 2b R; treatment and/or prevention of a proliferative disorder or disease or cancer or immune-related disorder or disease or condition; or treatment of cancer together with other clinically relevant cytotoxic agents or non-cytotoxic agents.

›SUMMARY OF THE INVENTION · 2 of 2

These and other features, aspects, and advantages of the present subject matter will become better understood with reference to the following description. This summary is provided to introduce a selection of concepts in a simplified form. This summary is not intended to identify key features or essential features of the disclosure, nor is it intended to be used to limit the scope of the subject matter.

›BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 depicts the in vivo efficacy effect of Example-13 on Tumor volume, in accordance with an implementation of the present disclosure.

FIG. 2 depicts Tumor Granzyme B expression compared to vehicle treatment in accordance with an implementation of the present disclosure.

›DETAILED DESCRIPTION

Those skilled in the art will be aware that the present disclosure is subject to variations and modifications other than those specifically described. It is to be understood that the present disclosure includes all such variations and modifications. The disclosure also includes all such steps, features, compositions, and compounds referred to or indicated in this specification, individually or collectively, and any combinations of any or more of such steps or features.

›Definitions · 1 of 7

For convenience, before further description of the present disclosure, certain terms employed in the specification, and examples are collected here. These definitions should be read in the light of the remainder of the disclosure and understood as by a person of skill in the art. The terms used herein have the meanings recognized and known to those of skill in the art, however, for convenience and completeness, particular terms and their meanings are set forth below.

The articles “a”, “an” and “the” are used to refer to one or to more than one (i.e., to at least one) of the grammatical object of the article.

The terms “comprise” and “comprising” are used in the inclusive, open sense, meaning that additional elements may be included. Throughout this specification, unless the context requires otherwise the word “comprise”, and variations, such as “comprises” and “comprising”, will be understood to imply the inclusion of a stated element or step or group of element or steps but not the exclusion of any other element or step or group of element or steps.

The term “including” is used to mean “including but not limited to”. “Including” and “including but not limited to” are used interchangeably.

In the structural Formulae given herein and throughout the present disclosure, the following terms have been indicated meaning, unless specifically stated otherwise.

In this specification, the prefix C x-y as used in terms such as C x-y alkyl and the like (where x and y are integers) indicates the numerical range of carbon atoms that are present in the group; for example, C 1-6 alkyl includes C 1 alkyl (methyl(-CH 3 ), (—CH 2 —)), C 2 alkyl (ethyl(-C 2 H 5 , —CH 2 CH 2 —)), C 3 alkyl (propyl and isopropyl) and C 4 alkyl (butyl, 1-methylpropyl, 2-methylpropyl, and t-butyl). Unless specifically stated, the bonding atom of a group may be any suitable atom of that group; for example, propyl includes prop-1-yl and prop-2-yl.

The term “alkyl” refers to a monoradical branched or unbranched saturated hydrocarbon chain having from 1 to 6 carbon atoms. This term is exemplified by groups such as methyl, ethyl, n-propyl, isopropyl, n-butyl, iso-butyl, t-butyl, n-hexyl, and the like. The groups may be optionally substituted.

The term “haloalkyl” as used herein refers to an alkyl group in which one or more hydrogen atoms are replaced by the same number of identical or different halogen atoms. The term “haloalkyl” is exemplified by groups such as chloromethyl, trifluoromethyl, 1-fluoroethyl, 2,2,2-trifluoroethyl, 2-fluoropropyl, 2,2-difluoropropyl, and the like. The term haloalkyl is exemplified by groups such as CF 3 , CHF 2 , CH 2 F, and the like.

The term “aminoalkyl” refers to a group having an amine and an alkyl group and the amine may be primary, secondary or tertiary amine. The point of attachment may be N or C i.e amine or alkyl group. The term “aminoalkyl” and “alkylamino” may be used interchangeably. The term aminoalkyl is exemplified by groups such as —CH(CH 3 ) 2 NH 2 , —CH 2 CH 2 NH 2 , —CH 2 CH 2 NHCH 3 , —CH 2 CH(NH 2 )CH 3 , —CH 2 CH 2 N(CH 3 ) 2 , —NHCH 3 , —N(CH 3 ) 2 , —CH 2 NH 2 , —CH 2 NHCH 3 , —CH 2 NHCH 2 —, —CH 2 NHCH 2 CH 2 — and the like.

The term “alkylhydroxy” or “alkylhydroxyl” refers to an alkyl group in which one or more hydrogen atoms are replaced by a hydroxyl(-OH) group. The term alkylhydroxy is exemplified by groups such as —CH 2 CH(CH 3 ) 2 OH, —CH(CH 3 ) 2 OH, —CH 2 OH, CH 2 CH 2 OH and the like.

The term “alkenyl” refers to a monoradical of a branched or unbranched unsaturated hydrocarbon group preferably having from 2, 3, 4, 5, or 6 carbon atoms and having 1, 2, or 3, double bond. The groups may be optionally substituted.

The term “alkynyl” refers to a monoradical of a branched or unbranched unsaturated hydrocarbon group preferably having from 2, 3, 4, 5, or 6 carbon atoms and having 1, 2, or 3, triple bond. The groups may be optionally substituted.

The term “cycloalkyl” refers to carbocyclic groups with 3 to 6 carbon atoms having a single cyclic ring or multiple condensed rings. Such cycloalkyl groups include, by way of example, single ring structures such as cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, and the like. The groups may be optionally substituted.

The term “cycloalkenyl” refers to carbocyclic groups of 3 to 6 carbon atoms having a single cyclic ring or multiple condensed rings which may be partially unsaturated.

The term “halocycloalkyl” as used herein refers to a cycloalkyl substituted with one or more halogen atoms. The halocycloalkyl refers to 3 to 6 carbon containing cycloalkyl substituted with one or more halogens.

The terms “alkoxyl” or “alkoxy” refers to an alkyl group, as defined above, having an oxygen radical attached thereto. Representative alkoxyl groups include methoxy, ethoxy, propyloxy, tert-butoxy, and the like. An “ether” is two hydrocarbons covalently linked by an oxygen. Accordingly, the substituent of an alkyl that renders an ether is or resembles an alkoxyl, such as can be represented by one of —O-alkyl, —O-alkenyl, —O-alkynyl. The alkoxy groups may be optionally substituted.

“Halo” or “Halogen”, alone or in combination with any other term means halogens such as chloro (Cl), fluoro (F), bromo (Br) and iodo (I).

The term “carbocyclyl” or “carbocycle” refers to a saturated, unsaturated ring having 4 to 10 carbon atoms forming cyclic systems. Carbocyclic groups may be spiral or bridged systems, may be saturated, unsaturated or partially saturated. Carbocyclyl groups may be optionally substituted with one or more heteroatoms. Carbocyclic groups may refer to heteroaryl groups with one or more heteroatoms. Representative carbocycles include cyclopropyl, cyclobutyl, cyclopentyl, 1-cyclopent-1-enyl, 1-cyclopent-2-enyl, 1-cyclopent-3-enyl, cyclohexyl, 1-cyclohex-1-enyl, 1-cyclohex-2-enyl, 1-cyclohex-3-enyl, and the like.

The term “heterocyclyl” refers to a “carbocyclyl” as defined herein, wherein one or more carbon atoms have been replaced with a heteroatom selected from O, N, or S. The heterocyclyl group may contain 1 to 10 carbon in the ring structure, substituted with one to four heteroatoms selected from O, N, or S. The heterocyclyl may be saturated, unsaturated or partially saturated. Representative examples of heterocyclyl include oxiranyl, oxetanyl, oxolanyl, oxanyl, furanyl, dioxanyl, pyranyl, aziridinyl, piperidinyl, tetrahydropyranyl, azepinyl, oxazepinyl and the like.

›Definitions · 2 of 7

The term “heteroaryl” refers to aromatic rings containing from 1 to 4 heteroatoms selected from N, O and S in the ring. “Heteroaryl” groups may be substituted with one or more substituents if so defined herein. The “C 2-6 heteroaryl” rings refers to a group having 2 or 6 carbon as ring member atoms with one to four heteroatoms.

The term “aryl” refers to aromatic ring having a specified number of carbon atoms. For example, C 5-10 aryl refers to an aryl group having 5 to 10 member atoms, or 6 member atoms. Preferred aryl groups include, without limitation, phenyl, and the like.

The term “haloalkoxy” refers to an alkoxy group as defined above further attached via halo linkage. For example, C 1-6 haloalkoxy refers to an alkoxy group having 1-6 carbon atoms further attached to one or more halogen. Preferred haloalkoxy groups include, without limitation, —CH 2 OCF 3 , —CH 2 CH 2 OCF 3 , —CH 2 CH 2 OCHF 2 , —OCH 2 Cl, —OCHCl 2 , —CH 2 OCF 3 and the like.

As used herein, the term “substituted” is contemplated to include all permissible substituents of organic compounds. In a broad aspect, the permissible substituents include acyclic and cyclic, branched and unbranched, carbocyclic and heterocyclic, aromatic and nonaromatic substituents of organic compounds. Illustrative substituents include, for example, those described herein above. The permissible substituents can be one or more and the same or different for appropriate organic compounds. For purposes of this disclosure, the heteroatoms such as nitrogen may have hydrogen substituents and/or any permissible substituents of organic compounds described herein which satisfy the valences of the heteroatoms.

The term “effective amount” means an amount of a compound or composition which is sufficient enough to significantly and positively modify the symptoms and/or conditions to be treated (e.g., provide a positive clinical response). The effective amount of an active ingredient for use in a pharmaceutical composition will vary with the particular condition being treated, the severity of the condition, the duration of the treatment, the nature of concurrent therapy, the particular active ingredient(s) being employed, the particular pharmaceutically-acceptable excipient(s)/carrier(s) utilized, the route of administration, and like factors within the knowledge and expertise of the attending physician.

The compounds described herein may contain one or more chiral centers and/or double bonds and therefore, may exist as stereoisomers, such as double-bond isomers (i.e., geometric isomers), regioisomers, enantiomers, or diastereomers. Accordingly, the chemical structures depicted herein encompass all possible enantiomers and stereoisomers of the illustrated or identified compounds including the stereoisomerically pure form (e.g., geometrically pure, enantiomerically pure or diastereomerically pure) and enantiomeric and stereoisomeric mixtures. Enantiomeric and stereoisomeric mixtures can be resolved into their component enantiomers or stereoisomers using separation techniques or chiral synthesis techniques well known to the person skilled in the art. The compounds may also exist in several tautomeric forms including the enol form, the keto form, and mixtures thereof. Accordingly, the chemical structures depicted herein encompass all possible tautomeric forms of the illustrated or identified compounds.

The term “racemates” refers to a mixture comprising a pair of optical isomers. Racemate refers to equimolar mixture of a pair of enantiomers. Racemate does not exhibit optical activity.

The term “pharmaceutically acceptable” refers to those compounds, materials, compositions, and/or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit/risk ratio.

“Pharmaceutically acceptable salt” embraces salts with a pharmaceutically acceptable acid or base. Pharmaceutically acceptable acids include both inorganic acids, for example hydrochloric, sulphuric, phosphoric, diphosphoric, hydrobromic, hydroiodic and nitric acid and organic acids, for example citric, fumaric, maleic, malic, mandelic, ascorbic, oxalic, succinic, tartaric, benzoic, acetic, methanesulphonic, ethanesulphonic, benzenesulphonic or p-toluenesulphonic acid. Pharmaceutically acceptable bases include alkali metal (e.g. sodium or potassium) and alkali earth metal (e.g. calcium or magnesium) hydroxides and organic bases, for example alkyl amines, arylalkyl amines and heterocyclic amines.

The term “polymorphs” refers to crystal forms of the same molecule, and different polymorphs may have different physical properties such as, for example, melting temperatures, heats of fusion, solubilities, dissolution rates and/or vibrational spectra as a result of the arrangement or conformation of the molecules in the crystal lattice.

Salts and solvates having non-pharmaceutically acceptable counter-ions or associated solvents are within the scope of the present disclosure, for example, for use as intermediates in the preparation of other compounds of Formula I, and their pharmaceutically acceptable salts. Thus, one embodiment of the disclosure embraces compounds of Formula I, and salts thereof. Compounds according to and Formula I contain a basic functional group and are therefore capable of forming pharmaceutically acceptable acid addition salts by treatment with a suitable acid. Suitable acids include pharmaceutically acceptable inorganic acids and pharmaceutically acceptable organic acids. Representative pharmaceutically acceptable acid addition salts include hydrochloride, hydrobromide, nitrate, methylnitrate, sulfate, bisulfate, sulfamate, phosphate, acetate, hydroxyacetate, phenyl acetate, propionate, butyrate, iso-butyrate, valerate, maleate, hydroxymaleate, acrylate, fumarate, malate, tartrate, citrate, salicylate, glycollate, lactate, heptanoate, phthalate, oxalate, succinate, benzoate, o-acetoxybenzoate, chlorobenzoate, methylbenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, naphthoate, hydroxynaphthoate, mandelate, tannate, formate, stearate, ascorbate, palmitate, oleate, pyruvate, pamoate, malonate, laurate, glutarate, glutamate, estolate, methanesulfonate (mesylate), ethanesulfonate (esylate), 2-hydroxyethanesulfonate, benzenesulfonate (besylate), aminobenzenesulfonate, p-toluenesulfonate (tosylate), and naphthalene-2-sulfonate.

›Definitions · 3 of 7

The term “solvate”, as used herein, refers to a crystal form of a substance which contains solvent.

The term “complexes” as used herein, can be interchangeably used as “coordination complex,” or “metal coordination complex,” and the like. It refers to a complex of an organic compound with a metal that can be empirically differentiated from a simple metal salt of the organic compound based on physiochemical and/or spectroscopic properties, with a coordination complex typically having enhanced covalency as compared to a salt. Without limitation “complexes” as used herein also involve a combination of coordinate covalent bonds and/or ionic bonds. As used herein, the term “complexes” also includes molecules that lack an ionic component (e.g., such as a neutral coordination complex prior to deprotonation, where pKa of the coordination complex falls within a physiologically acceptable range).

The term “hydrate” refers to a solvate wherein the solvent is water.

A term once described, the same meaning applies for it, throughout the disclosure.

As discussed in the background, there has been enormous efforts for the identification and development of new compounds acting as antagonists for adenosine receptors for treating proliferative disorders or diseases or condition or diseases related to immune depression or cancer. Heterocyclic compounds have been found to act as such antagonists and in the present disclosure there is disclosed, compounds of Formula I, their synthetic preparation methods, and their biological activity towards the adenosine receptors. The compounds of Formula I are found to be adenosine receptor antagonists, in particular adenosine A 2a R and A 2b R receptor antagonists. The compounds of Formula I are suitably formulated with therapeutic agents to form the pharmaceutical composition. The pharmaceutical composition and the compounds of Formula I is used for the treatment of diseases or disorders or condition mediated by the adenosine receptors.

R 2 is selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, or C 1-6 haloalkyl; R 3 is selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, or C 1-6 haloalkyl; wherein C 1-6 alkyl is optionally substituted with C 1-6 alkoxy; R 4 and R 5 are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, —CO—N(R 10 R 11 ), C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 2-6 alkenyl or C 2-6 alkynyl are optionally substituted with one or more groups selected from C 3-6 cycloalkyl, C 1-6 alkoxy, or C 1-10 heterocyclyl; or R 4 and R 5 are joined together to form C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 4-10 carbocyclyl or C 1-10 heterocyclyl is optionally substituted with 1 to 3 groups independently selected from halogen, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy; R 10 and R 11 are independently selected from hydrogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 3-6 halocycloalkyl, C 4-10 carbocyclyl, or C 1-10 heterocyclyl; R 6 is selected from hydrogen, halogen, hydroxyl, C 1-6 alkyl, C 1-6 haloalkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 1-6 alkoxy, C 1-6 haloalkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 3-6 cycloalkyl, C 3-6 halocycloalkyl, —Y—O—C 1-6 alkyl, —Y—O—C 3-6 cycloalkyl, —Y—CO—NH—R 13 , —Y—Z, C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 1-6 alkoxy, C 3-6 cycloalkyl, C 1-6 haloalkyl, C 1-6 aminoalkyl, C 1-6 alkylhydroxy, or C 1-10 heterocyclyl is optionally substituted with one or more groups selected from halogen, hydroxyl, amine, C 1-6 alkoxy, C 3-6 cycloalkyl, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 1-6 haloalkoxy, C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 3-6 cycloalkyl, or C 1-6 aminoalkyl, is optionally further substituted with one or more groups independently selected from halogen, hydroxyl, C 1-6 alkylhydroxy, —C(O)C 1-6 alkyl, —C(O)NH 2 , —C(O)—C 1-6 alkylhydroxy, or C 1-10 heterocyclyl, and C 1-10 heterocyclyl is optionally substituted with 1 to 3 groups independently selected from halogen, hydroxyl, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy; Y is selected from C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-6 cycloalkyl, or C 3-6 cycloalkenyl; and C 1-6 alkyl is optionally substituted with C 3-6 cycloalkyl; Z is selected from —OH, —NH 2 , —COOH, —SO 2 NH 2 , —SO 2 CH 3 , —SOCH 3 , —SCH 3 , CH 2 —NH—(C 1-6 alkyl)—SOCH 3 , —O—P(═O)(OH) 2 , —CONH 2 , —CONH(C 1-6 alkyl), —SO 2 NH(C 1-6 alkyl), CON(C 1-6 alkyl) 2 , —NHCO(C 1-6 alkyl), C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 1-10 heterocyclyl has 1 to 4 heteroatoms independently selected from O, N or S, and optionally substituted with 1 to 3 groups independently selected from halogen, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy; R 13 is selected from hydrogen, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl; R 7 , R 8 , R 9 , and R 9a are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl; and n is 0, 1, or 2.

In an embodiment of the present disclosure, there is provided a compound of Formula I or its pharmaceutically acceptable salts, complexes, hydrates, solvates, tautomers, polymorphs, stereoisomers, racemates, pharmaceutically active derivatives thereof, A is selected from C 5-10 aryl, C 2-10 heteroaryl, or C 1-10 heterocyclyl; C 5-10 aryl, C 2-10 heteroaryl, or C 1-10 heterocyclyl is optionally substituted with one or more substituents selected from R 6 , R 7 , R 8 , R 9 , R 9a , or oxo; R 2 is selected from hydrogen, C 1-6 alkyl, or C 1-6 haloalkyl; R 3 is selected from hydrogen, C 1-6 alkyl, or C 1-6 haloalkyl and wherein C 1-6 alkyl is optionally substituted with C 1-6 alkoxy; R 4 and R 5 are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, C 4-10 carbocyclyl, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 2-6 alkenyl, or C 2-6 alkynyl, is optionally substituted with one or more groups selected from C 3-6 cycloalkyl, C 1-6 alkoxy, or C 1-10 heterocyclyl; wherein C 1-10 heterocyclyl has 1 to 3 heteroatoms independently selected from O, N or S, and optionally substituted with 1 to 3 groups independently selected from halogen, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy; R 6 is selected from hydrogen, halogen, hydroxyl, C 1-6 alkyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 haloalkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 3-6 cycloalkyl, C 3-6 halocycloalkyl, —Y—O—C 1-6 alkyl, —Y—O—C 3-6 cycloalkyl, —Y—CO—NH—R 13 , —Y—Z, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 haloalkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 3-6 cycloalkyl, C 3-6 halocycloalkyl or C 1-10 heterocyclyl is optionally substituted with one or more groups selected from halogen, hydroxyl, amine, C 1-6 alkoxy, C 3-6 cycloalkyl, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 1-6 haloalkoxy, or C 1-10 heterocyclyl, wherein C 3-6 cycloalkyl, or C 1-6 aminoalkyl, is optionally further substituted with one or more groups selected from halogen, hydroxyl, C 1-6 alkylhydroxy, —C(O)C 1-6 alkyl, —C(O)NH 2 , —C(O)—C 1-6 alkylhydroxy, or C 1-10 heterocyclyl; Y is selected from C 1-6 alkyl, or C 3-6 cycloalkyl; and C 1-6 alkyl is optionally substituted with C 3-6 cycloalkyl; Z is selected from —OH, —NH 2 , —COOH, —SO 2 NH 2 , —SO 2 CH 3 , —SOCH 3 , —SCH 3 , CH 2 —NH—(C 1-6 alkyl)—SOCH 3 , —O—P(═O)(OH) 2 , —CONH 2 , —CONH(C 1-6 alkyl), —SO 2 NH(C 1-6 alkyl), CON(C 1-6 alkyl) 2 , NHCO(C 1-6 alkyl), or C 1-10 heterocyclyl, wherein C 1-10 heterocyclyl has 1 to 3 heteroatoms independently selected from O, N, or S, and optionally substituted with 1 to 3 groups independently selected from halogen, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy; R 13 is selected from hydrogen, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl; R 7 , R 8 , R 9 , and R 9a are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl; and n is 0, 1, or 2.

›Definitions · 4 of 7

* is the point of attachment.

wherein Q is N or CR 1 ; and R 1 is selected from cyano, hydrogen, halogen, C 1-6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl C 1-6 alkoxy, C 1-6 haloalkyl, or C 1-6 haloalkoxy; and C 1-6 alkyl, C 2-6 alkenyl, or C 2-6 alkynyl is optionally substituted with C 1-6 alkoxy.

* is the point of attachment.

Q is N or CR 1 ;

R 1 is selected from cyano, hydrogen, halogen, C 1-6 alkyl, C 1-6 alkoxy, C 1-6 haloalkyl, or C 1-6 haloalkoxy; R 2 is hydrogen or C 1-6 alkyl; R 3 is hydrogen or C 1-6 alkyl; wherein C 1-6 alkyl is optionally substituted with C 1-6 alkoxy; R 4 and R 5 are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, C 1-10 heterocyclyl; wherein C 1-6 alkyl is optionally substituted with one or more groups selected from C 3-6 cycloalkyl, C 1-6 alkoxy, or C 1-10 heterocyclyl, wherein C 1-10 heterocyclyl has 1 to 3 heteroatoms independently selected from O, N or S and optionally substituted with 1 to 3 groups independently selected from halogen, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy; R 6 is selected from hydrogen, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, —Y—CO—NH—R 13 , —Y—Z, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 aminoalkyl, C 1-6 alkylhydroxy, or C 1-10 heterocyclyl is optionally substituted with one or more groups selected from halogen, hydroxyl, amine, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 1-6 haloalkoxy, or C 1-10 heterocyclyl having 1 to 4 heteroatoms independently selected from O, N or S, wherein C 3-6 cycloalkyl, or C 1-6 aminoalkyl optionally substituted with 1 to 3 groups independently selected from halogen, hydroxyl, C 1-6 alkyl, C 1-6 alkoxy, C 1-6 haloalkoxy, C 1-6 alkylhydroxy, —C(O)C 1-6 alkyl, —C(O)NH 2 , —C(O)—C 1-6 alkylhydroxy, or C 1-10 heterocyclyl; Y is C 1-6 alkyl or C 3-6 cycloalkyl, wherein C 1-6 alkyl optionally substituted with C 3-6 cycloalkyl; Z is selected from —OH, —NH 2 , —COOH, —SO 2 NH 2 , —SO 2 CH 3 , —S(═O)CH 3 , —SCH 3 , CH 2 —NH—(C 1-6 alkyl)—SOCH 3 , —O—P(═O)(OH) 2 , CONH 2 , CON(C 1-6 alkyl) 2 -CONH(C 1-6 alkyl), —SO 2 NH(C 1-6 alkyl), or —NHCO(C 1-6 alkyl); R 13 is selected from hydrogen, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl; R 7 , R 8 , R 9 , and R 9a are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl; and n is 0, 1 or 2.

* is the point of attachment, wherein Q is N or CR 1 ; R 1 is selected from cyano, hydrogen, halogen, C 1-6 alkyl, C 1-6 alkoxy, C 1-6 haloalkyl, or C 1-6 haloalkoxy; R 2 is hydrogen or C 1-6 alkyl; R 3 is hydrogen or C 1-6 alkyl and wherein C 1-6 alkyl is optionally substituted with C 1-6 alkoxy; R 4 and R 5 are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, —CO—N(R 10 R 11 ), C 1-10 heterocyclyl, wherein C 1-6 alkyl optionally substituted with one or more groups selected from C 3-6 cycloalkyl, C 1-6 alkoxy, or C 1-10 heterocyclyl, wherein the C 1-10 heterocyclyl has 1 to 4 heteroatoms independently selected from O, N or S and optionally substituted with 1 to 3 groups independently selected from halogen, C 1-6 alkyl, C 1-6 alkoxy, or C 1-6 haloalkoxy; R 10 is hydrogen or C 1-6 alkyl; R 11 is selected from hydrogen, C 1-6 alkyl, or C 1-10 heterocyclyl, wherein C 1-6 alkyl is optionally substituted with one or more groups selected from halogen, C 3-6 cycloalkyl or C 1-10 heterocyclyl; C 1-10 heterocyclyl has 1 to 4 heteroatoms independently selected from O, N or S, and optionally substituted with 1 to 3 groups independently selected from halogen, C 1-6 alkyl, C 1-6 alkoxy, C 1-6 haloalkoxy,

R 6 is selected from hydrogen, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, —Y—CO—NH—R 13 , —Y—Z, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 aminoalkyl, C 1-6 alkylhydroxy, or C 1-10 heterocyclyl is optionally substituted with one or more groups selected from halogen, hydroxyl, amine, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 1-6 haloalkoxy, C 1-10 heterocyclyl having 1 to 4 heteroatoms independently selected from O, N or S; wherein C 3-6 cycloalkyl, or C 1-6 aminoalkyl is optionally further substituted with one or more groups selected from halogen, hydroxyl, C 1-6 alkyl, C 1-6 alkoxy, C 1-6 haloalkoxy, C 1-6 alkylhydroxy, —C(O)C 1-6 alkyl, —C(O)NH 2 , —C(O)—C 1-6 alkylhydroxy, C 1-10 heterocyclyl, cyclopropyl, cyclobutyl, CH 2 CF 3 , CH 2 CHFCH 3 , CH 2 CF 2 CH 3 , CH 2 C(CH 3 ) 2 OH, C(CH 3 ) 2 OH, C(CD 3 ) 2 OH, CH 2 OH, CH 2 OCH 3 , CH 2 OCF 3 , C(CH 3 CF 3 )OH, CH 2 CH 2 OH, CH 2 CH 2 OCH 3 , CH 2 CH(OCH 3 )CH 3 , C(CH 3 ) 2 NH 2 , CH 2 NH 2 , CH 2 CH 2 NH 2 , CH 2 CH 2 NHCH 3 , CH 2 CH(NH 2 )CH 3 , CH 2 CH 2 N(CH 3 ) 2 , CH 2 CHFCH 2 NH 2 , OCH 3 , OCF 3 , OCH 2 CF 3 , OCH 2 CH 2 OCH 3 , OCH 2 CH 2 OCHCF 2 , OCH 2 CH 2 OH, OCH 2 CH 2 OCF 3 , CH 2 CF 2 CH 2 OH, CH 2 C(CH 3 ) 2 CH 2 OH, CHCF 3 OH, CHOHCH 2 OH, CHOHCH 2 OCH 3 , CH 2 CH 2 CH 2 OH, CH 2 CH 2 NHCH 2 CH 2 OH, CH 2 NHCH 2 CH 2 OH,CH 2 CH 2 NHCOCH 3 , CH 2 CH 2 S(═O)CH 3 , CH 2 CH 2 CH 2 S(═O)CH 3 ,

›Definitions · 5 of 7

Y is C 1-6 alkyl or C 3-6 cycloalkyl, wherein C 1-6 alkyl optionally substituted with C 3-6 cycloalkyl; Z is selected from —OH, —NH 2 , —COOH, —SO 2 NH 2 , —SO 2 CH 3 , —S(═O)CH 3 , —SCH 3 , CH 2 —NH—(C 1-6 alkyl)—SOCH 3 , —O—P(═O)(OH) 2 , CONH 2 , CON(C 1-6 alkyl) 2 -CONH(C 1-6 alkyl), —SO 2 NH(C 1-6 alkyl), or —NHCO(C 1-6 alkyl); and R 13 is selected from hydrogen, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl; R 7 , R 8 , R 9 , and R 9a are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, C 1-6 haloalkoxy, or C 3-6 halocycloalkyl; and n is 0, 1 or 2.

Q is N or CR 1 ; R 1 is selected from cyano, hydrogen, halogen, C 1-6 alkyl, or C 1-6 alkoxy; R 2 is hydrogen or C 1-6 alkyl; R 3 is hydrogen or C 1-6 alkyl; R 4 and R 5 are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 haloalkyl, or C 1-6 haloalkoxy; wherein C 1-6 alkyl optionally substituted with one or more groups selected from C 3-6 cycloalkyl, or C 1-6 alkoxy; R 6 is selected from hydrogen, C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, —Y—CO—NH—R 13 , —Y—Z, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 3-6 cycloalkyl, C 1-6 haloalkyl, C 1-6 alkoxy, C 1-6 aminoalkyl, C 1-6 alkylhydroxy, or C 1-10 heterocyclyl is optionally substituted with one or more groups independently selected from halogen, hydroxyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, C 1-6 haloalkoxy, C 1-10 heterocyclyl, wherein C 3-6 cycloalkyl, or C 1-6 aminoalkyl is optionally further substituted with one or more groups independently selected from halogen, hydroxyl, C 1-6 alkylhydroxy, —C(O)C 1-6 alkyl, —C(O)NH 2 , or —C(O)—C 1-6 alkylhydroxy; Y is C 1-6 alkyl or C 3-6 cycloalkyl; Z is selected from —OH, —NH 2 , —COOH, —SO 2 NH 2 , —SO 2 CH 3 , —SOCH 3 , —SCH 3 , CH 2 —NH—(C 1-6 alkyl)—SOCH 3 , —CONH 2 , —CONH(C 1-6 alkyl), —SO 2 NH(C 1-6 alkyl), CON(C 1-6 alkyl) 2 , or —NHCO(C 1-6 alkyl); R 13 is selected from hydrogen, C 1-6 alkyl, or C 3-6 cycloalkyl; R 7 , R 8 , R 9 , and R 9a are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl, C 3-6 cycloalkyl, or C 1-6 alkoxy; and n is 0, 1, or 2.

Q is N;

R 2 is hydrogen or C 1-6 alkyl; R 3 is hydrogen or C 1-6 alkyl; R 4 and R 5 are independently selected from halogen, C 1-6 alkyl, C 3-6 cycloalkyl or C 1-6 haloalkyl; wherein C 1-6 alkyl is optionally substituted with C 3-6 cycloalkyl; R 6 is selected from hydrogen, C 1-6 alkyl, C 1-6 haloalkyl, C 3-6 cycloalkyl, C 1-6 alkoxy, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, —Y—CO—NH—R 13 , —Y—Z, or C 1-10 heterocyclyl, wherein C 1-6 alkyl, C 1-6 alkoxy, C 3-6 cycloalkyl, C 1-6 haloalkyl, C 1-6 aminoalkyl, C 1-6 alkylhydroxy, or C 1-10 heterocyclyl is optionally substituted with one or more groups selected from halogen, hydroxyl, amine, C 1-6 alkoxy, C 3-6 cycloalkyl, C 1-6 alkylhydroxy, C 1-6 aminoalkyl, or C 1-10 heterocyclyl; wherein C 3-6 cycloalkyl, or C 1-6 aminoalkyl, is optionally further substituted with one or more groups independently selected from halogen, hydroxyl, C 1-6 alkylhydroxy, —C(O)C 1-6 alkyl, —C(O)NH 2 , or —C(O)—C 1-6 alkylhydroxy; Y is C 1-6 alkyl, or C 3-6 cycloalkyl;

Z is selected from —SOCH 3 , —SCH 3 , CH 2 —NH—(C 1-6 alkyl)—SOCH 3 , or —NHCO(C 1-6 alkyl); R 13 is selected from hydrogen, C 1-6 alkyl, or C 3-6 cycloalkyl; R 7 , R 8 , R 9 , and R 9a are independently selected from hydrogen, halogen, cyano, C 1-6 alkyl or C 1-6 alkoxy; and n is 0, or 1.

In an embodiment of the present disclosure, there is provided a process of preparation of compounds of Formula I or its pharmaceutically acceptable salts, complexes, hydrates, solvates, tautomers, polymorphs, stereoisomers, racemates, pharmaceutically active derivatives thereof as disclosed herein, said process comprising: reacting Formula (A), and Formula (B) in the presence of a base to obtain the compounds of Formula I

›Definitions · 6 of 7

wherein R is selected from C 1-6 alkyl, C 3-6 cycloalkyl, C 5-10 aryl, C 2-10 heteroaryl, or C 1-10 heterocyclyl; and the substituents are as disclosed herein.

In an embodiment of the present disclosure, there is provided a process of preparation of compounds of Formula I or its pharmaceutically acceptable salts, complexes, hydrates, solvates, tautomers, polymorphs, stereoisomers, racemates, pharmaceutically active derivatives thereof as disclosed herein, wherein the base is selected from triethylamine, diisopropylethylamine, pyridine, sodium carbonate, potassium carbonate, sodium hydroxide, potassium tertiarybutoxide potassium tertiarybutoxide, sodium hydride, lithium bis(trimethylsilyl)amide (LiHMDS), N-diisopropylethylamine, or combinations thereof.

In an embodiment of the present disclosure, there is provided a process of preparation of compounds of Formula I or its pharmaceutically acceptable salts, complexes, hydrates, solvates, tautomers, polymorphs, stereoisomers, racemates, pharmaceutically active derivatives thereof as disclosed herein, wherein the process is carried out in the presence of a solvent selected from isopropanol, methanol, n-butanol, dichloromethane, tetrahydrofuran, dimethylformaide, n-methylpyrrolidone, dimethyl sulfoxide, water, dioxane, acetonitrile, or combinations thereof.

In an embodiment of the present disclosure, there is provided a pharmaceutical composition comprising compounds of Formula I as disclosed herein and one or more additional therapeutic agent.

In an embodiment of the present disclosure, there is provided a pharmaceutical composition comprising compounds of Formula I with one or more additional therapeutic agent selected from chemotherapeutic agent, immune checkpoint inhibitors or combinations thereof. In another embodiment of the present disclosure, there is a pharmaceutical composition comprising compounds of Formula I as disclosed herein wherein the one or more additional therapeutic agent is chemotherapeutic agent or immune checkpoint inhibitors.

In an embodiment of the present disclosure, there is provided a pharmaceutical composition as disclosed herein, wherein the one or more additional therapeutic agent is the chemotherapeutic agent selected from phosphoinositide 3-kinase inhibitor (PI3K) inhibitor, tyrosine kinase inhibitor, signal transducer and activator of transcription 3 (Stat-3) inhibitor, topoisomerase inhibitors, Protein kinase B (AKT) inhibitor, c-Jun N-terminal kinase (JNK1/K2) inhibitors, hypoxia-inducible factor 1 alpha (HIF-1a) inhibitor, extracellular signal-regulated kinase (ERK) inhibitor, poly ADP ribose polymerase-1((PARP-1) inhibitor, cisplatin, or oxaplatin.

In an embodiment of the present disclosure, there is provided a pharmaceutical composition as disclosed herein, wherein the one or more additional therapeutic agent is the immune checkpoint inhibitor selected from programmed death-1 (PD-1) inhibitor, programmed death-ligand 1 (PD-L1) inhibitor, anti-PD1 antibody, anti-PD-L1 antibody, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) inhibitor, anti-CTLA-4 antibody, T cell immunoglobulin and mm domain (TIGIT) inhibitor, ecto-nucleoside triphosphate diphosphohydrolase 1(E-NTPDase, CD39) inhibitor, or ecto-5′-nucleotidase(Ecto5′NTase, CD79) inhibitor.

In an embodiment of the present disclosure, there is provided a pharmaceutical composition comprising the compounds of Formula I or its pharmaceutically acceptable salts, complexes, hydrates, solvates, tautomers, polymorphs, stereoisomers, pharmaceutically active derivatives thereof with one or more additional therapeutic agent selected from chemotherapeutic agent or immune checkpoint inhibitors and wherein the one or more additional therapeutic agent selected from phosphoinositide 3-kinase inhibitor (PI3K) inhibitor, tyrosine kinase inhibitor, signal transducer and activator of transcription 3 (Stat-3) inhibitor, topoisomerase inhibitors, Protein kinase B (AKT) inhibitor, c-Jun N-terminal kinase (JNK1/K2) inhibitors, hypoxia-inducible factor 1 alpha (HIF-1a) inhibitor, extracellular signal-regulated kinase (ERK) inhibitor, poly ADP ribose polymerase-1((PARP-1) inhibitor, cisplatin, oxaplatin, programmed death-1 (PD-1) inhibitor, programmed death-ligand 1 (PD-L1) inhibitor, anti-PD1 antibody, anti-PD-L1 antibody, cytotoxic T-lymphocyte-associated protein 4 (CTLA-4)inhibitor, anti-CTLA-4 antibody, T cell immunoglobulin and ITIM domain (TIGIT) inhibitor, ecto-nucleoside triphosphate diphosphohydrolase 1(E-NTPDase, CD39) inhibitor, or ecto-5′-nucleotidase(Ecto5′NTase, CD79) inhibitor.

›Definitions · 7 of 7

In an embodiment of the present disclosure, there is provided a method of treatment and/or prevention of a condition mediated by adenosine receptor or a proliferative disorder or disease or cancer, comprising administering to a subject suffering from a condition mediated by adenosine receptor or a disease or proliferative disorder or cancer, a therapeutically effective amount of the compounds of Formula I as disclosed herein or the pharmaceutical composition as disclosed herein, wherein the cancer is of adrenal gland, brain, bladder, breast, bone, colon, endometrial, oesophagus, head, gastric, kidney, liver, lung, mouth, muscle, neck, pancreas, prostate, retinal, skin, thyroid or white blood cells and said immune related disease, disorder or condition is selected from the group consisting of allergies, alzheimer, asthma, crohn's disease, colitis, chronic obstructive pulmonary disease, diabetic kidney disorders, glaucoma, lupus, rheumatoid arthritis, multiple sclerosis, pain, panic disorder, pancreatitis, parkinson disease, psoriasis, systemic sclerosis, and ulcerative colitis.

In an embodiment of the present disclosure, there is provided a use of the compounds of Formula I as disclosed herein or the pharmaceutical composition as disclosed herein for treatment of a condition mediated by adenosine receptor A 2a R; treatment and/or prevention of a proliferative disorder or disease or cancer or immune related disorder or disease or condition; or treatment of cancer together with other clinically relevant cytotoxic agents or non-cytotoxic agents.

In an embodiment of the present disclosure, there is provided a use of the compounds of Formula I as disclosed herein or the pharmaceutical composition as disclosed herein for treatment of a condition mediated by adenosine receptor A 2b R; treatment and/or prevention of a proliferative disorder or disease or cancer or immune related disorder or disease or condition; or treatment of cancer together with other clinically relevant cytotoxic agents or non-cytotoxic agents.

In an embodiment of the present disclosure, there is provided a use of the compounds of Formula I as disclosed herein or the pharmaceutical composition as disclosed herein for treatment of a condition mediated by adenosine receptors A 2a R and A 2b R; treatment and/or prevention of a proliferative disorder or disease or cancer or immune related disorder or disease or condition; or treatment of cancer together with other clinically relevant cytotoxic agents or non-cytotoxic agents.

›EXAMPLES

As used herein the symbols and conventions used in these processes, schemes and examples are consistent with those used in the contemporary scientific literature. Unless otherwise noted, all starting materials were obtained from commercial suppliers and used without further purification. Specifically, the following abbreviations may be used in the examples and throughout the specification:

›Abbreviations · 1 of 69

The following abbreviations are employed in the examples and elsewhere herein:

TLC—thin layer chromatography; HPLC—high pressure liquid chromatography; MPLC—medium pressure liquid chromatography; NMR—nuclear magnetic resonance spectroscopy; DMSO—dimethylsulfoxide; CDCl 3 —deuterated chloroform; MeOD—deuterated methanol, i.e. D 3 COD; MS—mass spectroscopy; ESP (or ES)—electrospray; EI—electron impact; APCI—atmospheric pressure chemical ionization; THF—tetrahydrofuran; DCM—dichloromethane; MeOH—methanol; DMF—dimethylformamide; EtOAc—ethyl acetate; LC/MS—liquid chromatography/mass spectrometry; h—hour(s); min is minute(s); d—day(s); MTBD—N-methyl-1,5,7-triazabicyclo[4.4.0]dec-5-ene; TFA—trifluoroacetic acid; v/v—ratio of volume/volume; Boc—t-butoxycarbonyl; Cbz—benzyloxycarbonyl; Bz—benzoyl; Atm—atmospheric pressure; rt—room temperature; mg—milligram; g denotes gram; μL—microliter; mL—milliliter; L—liter; μM—micromolar; nM—Nanomolar mM—millimolar; M denotes molar; DMAP—dimethyaminopyridine; TBDMS—tert-butyldimethylsilyl N—normal; and nm—nanometer.

The following examples provide the details about the synthesis, activities and applications of the compounds of the present disclosure. It should be understood the following is representative only, and that the invention is not limited by the details set forth in these examples.

Materials and Methods

Evaporations were carried out by rotary evaporation in vacuo and work up procedures were carried out after removal of residual solids by filtration; temperatures are quoted as ° C.; operations were carried out at room temperature, that is typically in the range 18 to 26° C. and without the exclusion of air unless otherwise stated, or unless the skilled person would otherwise work under an inert atmosphere; column chromatography (by the flash procedure) was used to purify compounds and was performed on Merck Kieselgel silica (Art. 9385) unless otherwise stated; in general, the course of reactions was followed by TLC, HPLC, or LC/MS and reaction times are given for illustration only; yields are given for illustration only and are not necessarily the maximum attainable; the structure of the end products of the invention was generally confirmed by NMR and mass spectral techniques. Proton magnetic resonance spectra were generally determined in DMSO d6 unless otherwise stated, using a Bruker DRX 300 spectrometer or a Bruker DRX-400 spectrometer, operating at a field strength of 300 MHz or 400 MHz, respectively. In cases where the NMR spectrum is complex, only diagnostic signals are reported. Chemical shifts are reported in parts per million downfield from tetramethylsilane as an external standard (* scale) and peak multiplicities are shown thus: s, singlet; d, doublet; dd, doublet of doublets; dt, doublet of triplets; dm, doublet of multiplets; t, triplet, m, multiplet; br, broad. Fast atom bombardment (FAB) mass spectral data were generally obtained using a Platform spectrometer (supplied by Micromass) run in electrospray and, where appropriate, either positive ion data or negative ion data were collected or using Agilent 1100 series LC/MS equipped with Sedex 75ELSD, and where appropriate, either positive ion data or negative ion data were collected. The lowest mass major ion is reported for molecules where isotope splitting results in multiple mass spectral peaks (for example when chlorine is present). Reverse Phase HPLC was carried out using YMC Pack ODS AQ (100×20 mmID, S 5 Å particle size, 12 nm pore size) on Agilent instruments; each intermediate was purified to the standard required for the subsequent stage and was characterized in sufficient detail to confirm that the assigned structure was correct; purity was assessed by HPLC, TLC, or NMR and identity was determined by infrared spectroscopy (IR), mass spectroscopy or NMR spectroscopy as appropriate.

General Process for the Preparation of the Compounds of Formula (A)

Compounds of formula (A) was prepared from formula (F) via oxidation using appropriate oxidizing agents (e.g., mCPBA) and the compounds of formula (F) was synthesized by reacting compounds of formula (D) with formula (E) in presence of appropriate base (e.g., pyridine). The formula (D) was obtained by treating formula (C) with carbon disulphide and methyl iodide in presence of appropriate base (e.g., sodium hydride) (Scheme 1).

General Process for the Preparation of the Compounds of Formula B

The preparation of compounds of Formula (B) may be prepared in a variety of ways and many of them are commercially available. The generic route for Formula (B) is given Scheme 2 wherein, the formula (B) was obtained by treating a carbonyl compound of Formula (B1) with an amine of Formula (B2) with suitable reducing reagent.

Further, the processes for one of the representative compounds of formula(B) wherein A is six membered rings shown as formula (N) in scheme 3.

The compounds of Formula (B) were obtained by reducing either compounds of Formula (H) or (J) with appropriate reducing agents. The compounds of Formula (H) were obtained by treating compounds Formula (G) with nitromethane wherein Q is an aldehyde. The compounds of Formula (J) were obtained by treating compounds of Formula (G) with acetonitrile in presence appropriate alkali metal base (E.g., nBuLi) where in Q is a halogen (E.g., Br or I).

In similar to the process of making compounds of Formula(I) the

processes for one of the representative compounds of Formula(B) with five membered rings shown as Formula (M) in scheme 4.

The examples shown above illustrate some methods useful for the synthesis of compounds of Formula (A) and compound (B) which may be used for the synthesis of compounds of Formula (I). Where a particular solvent or reagent is shown or referred to in the accompanying text, it is to be understood that the chemist of ordinary skill in the art will be able to modify and/or replace that solvent or reagent, as necessary.

In another embodiment, the compounds of Formula I, can be prepared reacting compounds of Formula (B) with compounds of Formula (A), in the presence of appropriate base and solvents as shown scheme 5.

›Abbreviations · 2 of 69

The present disclosure provides a process for the preparation of compounds of Formula (I) and the compounds of Formula (I) which may be prepared in a variety of ways. The processes and examples shown below illustrate some methods useful for the synthesis of compounds of Formula (I) and intermediates which may be used for the synthesis of compounds of Formula (I). Where a particular solvent or reagent is shown or referred to in the accompanying text, it is to be understood that the chemist of ordinary skill in the art will be able to modify and/or replace that solvent or reagent as necessary

Synthesis of Intermediates

Synthesis of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile. (I)

Step-1: Synthesis of 2-(bis(methylthio)methylene) malononitrile (Ia)

A stirred solution of KOH (16.98 g, 302.75 mmol) in water (100 mL) was cooled to 0° C. and was added malononitrile (10 g, 151.37 mmol) in dry THF (20 mL) was added dropwise over a period of 30 min under N 2 atmosphere. The reaction was stirred at room temperature for 1 h and was added CS 2 (17.2 g, 227.06 mmol) at 0° C. After stirring the reaction mixture at room temperature for about 1 h dimethylsulphate (24.8 g, 196.7 mmol) was added and stirring was continued for another 4 h at room temperature. After completion, the reaction mixture was quenched with ice cold water and the solid precipitated was filtered and dried under vacuo to afford the compound Ia (8 g, 31%) as yellow solid, which was taken forward for further step without purification.

Step-2: Synthesis of 7-amino-2,3-dimethyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (Ic)

To a stirred solution of Ia (10 g, 58.8 mmol) in ethanol (20 mL) was added Ib (4.57 g, 41.17 mmol) at room temperature under N 2 atmosphere. The resulted mixture was heated at 120° C. for 6 h. After that the reaction mixture was cooled to room temperature and the solid precipitated was filtered and washed with ethanol to give off-white solid, it was purified by column chromatography on silica gel (230-400 mesh, 45% ethyl acetate in pet ether) to obtained Ic (3.1 g, 32%); LC-MS Calculated. for C 10 H 11 N 5 S: 233.29; Observed.: 234.0; [M + +H]. 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.60 (s, 2H), 2.68 (s, 3H), 2.37 (s, 3H), 2.13 (s, 3H).

Step-3: Synthesis of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile. (I)

A stirred solution of Ic (5 g, 21.45 mmol) in dichloromethane (50 mL) was cooled to 0° C. and was added m-CPBA (11.07 g, 64.37 mmol) under N 2 atmosphere. The resulted mixture was stirred at room temperature for 3 h. After that the reaction mixture was quenched with 10% NaHCO 3 solution and extracted with DCM (2×300 mL). The combined organic layer was dried over Na 2 SO 4 and concentrated under vacuo to afford the compound I (3.1 g, 54%) as an off-white solid which was taken forward for further step without purification. LC-MS Calculated. for C 10 H 11 N 5 O 2 S: 265.29; Observed.: 266.0; [M + +H]. 1 H NMR (400 MHz, DMSO-D 6 ): δ 9.1 (br s, 2H), 3.42 (s, 3H), 2.40 (s, 3H), 2.17 (s, 3H).

Synthesis of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile. (II)

Step-1: 2-ethyl-3-oxobutanenitrile (IIa)

To a three neck RB flask, LDA (2M in THF, 36.17 ml, 0.0723 mol) was taken and THF (82.5 ml) was added and cooled to −78° C. To this solution, butyronitrile (5 g, 0.0723 mol) in THF (10 ml) was added dropwise at −78° C. and the reaction mixture was stirred at −78° C. for 1 h. Then, ethyl acetate (6.42 ml, 0.0657 mol) was added dropwise at −78° C. and the reaction mixture was stirred at −78° C. for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with saturated solution of NH 4 Cl (25 ml) and extracted with ethyl acetate (50 ml×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure to get the title compound IIa as yellow viscous oil; Yield: (8 g, 100%). 1 H NMR (400 MHz, CDCl 3 ): δ 3.237-3.205 (q, 1H), 2.499 (s, 3H), 1.153-1.137 (d, J=6.4 Hz, 3H), 0.973-0.937 (t, J=7.2 Hz, 3H).

Step-2: 4-ethyl-5-methyl-1H-pyrazol-3-amine (IIb)

To a stirred solution of 2-ethyl-3-oxobutanenitrile 3 (8 g) in EtOH (80 ml) was added hydrazine hydrate (23.80 ml) and the reaction mixture was heated at 120° C. for 1.5 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to rt and evaporated under reduced pressure. The residue was dissolved in 6N HCl (25 ml) and washed with ethyl acetate (3×50 ml). Aq. layer was basified to pH>7 using aqueous ammonia solution and extracted with ethyl acetate (3×50 ml). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure to get the title compound IIa as light brown viscous liquid was used in the next step without further purification; Yield: (8.1 g, 90%). 1 H NMR (400 MHz, CD3OD): δ 2.354 (q, J=7.2 Hz, 2H), 2.110 (s, 3H), 1.087-1.049 (t, J=7.2 Hz, 3H).

Step-3: 2-(Bis(methylthio) methylene) malononitrile (Ia)

To a stirred solution of malononitrile (5.0 g, 0.075 mol) in DMSO (50 ml) cooled to 0° C. was added K 2 CO 3 (11.4 g, 0.0833 mol), CS 2 (5.03 g, 0.0833 mol) and stirred at rt for 3 h. Then the reaction mixture cooled to 0° C. and MeI (9.4 ml, 0.1514 mol) was added and the reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was poured into ice cold water (50 ml) and the precipitate formed was filtered, washed with cold water and dried to get the compound Ia as brick red solid which was used in the next step without further purification; Yield: (6.2 g, 51%).

Step-4: 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (IIc)

To a stirred solution of 2-(bis(methylthio)methylene) malononitrile Ia (11.0 g, 0.0647 mol) in pyridine (80 ml) was added 4-ethyl-5-methyl-1H-pyrazol-3-amine (8.1 g, 0.0647 mol) and the reaction mixture was heated at 120° C. for 2.5 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated, and the residue was diluted with water (150 ml) and resulting solid was stirred for 2 h at 10-15° C. Filtered the solid and azeotroped with toluene (2×50 ml) to get dried title compound IIc. Yield: (12.3 g, 78%). 1 H NMR (400 MHz, CD 3 OD): δ 2.691-2.634 (q, J=7.6 Hz, 2H), 2.583 (s, 3H), 2.371 (s, 3H), 1.257-1.205 (t, J=8 Hz, 3H).

›Abbreviations · 3 of 69

Step-5: 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile. (II)

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile IIc (11.2 g, 0.0481 mol) in DCM (120 ml) cooled to 0° C., was added m-CPBA (33.20 g, 0.19 mol) slowly and the reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC (polar spot). After completion, the reaction mixture was quenched with NaHCO 3 (150 ml) and extracted with DCM (150 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified using 50% ethyl acetate in hexane to get pure title compound. Yield: (9.5 g, 70%). LC_MS Calculated for C 11 H 13 N 5 O 2 S: 279.10; Observed: 280.10 [M + +H]. 1 H NMR (400 MHz, CD 3 OD): δ 3.385 (s, 3H), 2.777-2.721 (q, J=7.2 Hz, 2H), 2.463 (s, 3H), 1.260-1.230 (t, J=7.2 Hz, 3H).

Synthesis of 7-amino-3-isopropyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile. (III)

Step-1: 2-acetyl-3-methylbutanenitrile. (Ma)

To a three neck RB flask, LDA (2M in THF, 29.8 mL, 0.0595 mol) was taken and THF (100 mL) was added and cooled to −78° C. To this solution, 3-methylbutanenitrile (CAS:625-28-5, 25 g, 0.0595 mol) in THF (40 mL) was added dropwise at −78° C. and the reaction mixture was stirred at −78° C. for 1 h. Then, ethyl acetate (CAS:141-78-6, 5.2 mL, 0.0536 mol) was added dropwise at −78° C. and the reaction mixture was stirred at −78° C. for 1 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with saturated solution of NH 4 Cl (50 mL) and extracted with ethyl acetate (80 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure to get the title compound Ma as yellow viscous oil; Yield: (6.5 g, 87%). LC_MS Calculated for C 7 H 11 NO: 125.08; Observed:124.10 [M−H].

Step-2: 4-isopropyl-5-methyl-1H-pyrazol-3-amine. (IIIb)

To a stirred solution of 2-acetyl-3-methylbutanenitrile Ma (6.5 g, 0.0520 mol) in EtOH (65 mL) was added hydrazine hydrate (19.5 mL, 0.00438 mol) and the reaction mixture was heated at 120° C. for 1.5 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to rt and evaporated under reduced pressure. The residue obtained was diluted with water (100 mL) and extracted with ethyl acetate (80 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure to get tittle compound IIIb as light brown viscous liquid. This crude product was used as such for the next step without further purification; Yield: (4.5 g, 52%). 1 H NMR (400 MHz, CDCl 3 ): δ 5.18 (bs, 2H), 3.18 (d, J=7.6 Hz, 1H), 2.29-2.04 (m, 1H), 1.83 (s, 3H), 1.22 (d, J=10.0 Hz, 1H), 1.11 (d, J=6.8 Hz, 3H), 1.01 (d, J=6.8 Hz, 3H).

Step-3: 2-(Bis(methylthio) methylene) malononitrile. (Ia)

Step-4: 7-amino-3-isopropyl-2-methyl-5-(methylthio) pyrazolo[1,5-a]pyrimidine-6-carbonitrile. (Mc)

To a stirred solution of 2-(bis(methylthio)methylene) malononitrile Ia (6.1 g, 0.0360 mol) in pyridine (50 mL) and 4-isopropyl-5-methyl-1H-pyrazol-3-amine IIIb (4.5 g, 0.0360 mol) and the reaction mixture was heated at 120° C. in for 1.5 h. The progress of the reaction was monitored by TLC. After completion, to the reaction 200 mL water was added and the pale-yellow residue formed was collected by filtration. The solid was washed with fresh water (200 mL) and dried under vacuo to afford the desired compound as a pale-yellow solid. The crude material was used in the next step without further purification; Yield: (6.0 g, 71%). LC_MS Calculated for C 12 H 15 N 5 S: 261.10; Observed: 262.10 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 6.20 (bs, 2H), 3.09-3.06 (m, 1H), 2.62 (s, 3H), 2.40 (s, 3H), 1.38 (d, J=6.8 Hz, 6H).

Step-5: 7-amino-3-isopropyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile. (III)

To a stirred solution of 7-amino-3-isopropyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile Mc (6.0 g, 0.023 mol) in DCM (60 mL), cooled to 0° C., was portion wise added m-CPBA (16.0 g, 0.092 mol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC (polar spot). After completion, the reaction mixture was quenched with NaHCO 3 (100 mL) and extracted with DCM (80 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure. The crude material was subjected to silica gel (100-200) column chromatography using ethyl acetate (0-30%) in n-hexane to afford the desired compound III as an off-white solid; Yield: (1.5 g, 22%). LC_MS Calculated for C 12 H 15 N 5 O 2 S: 293.09; Observed: 294.05 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 9.18 (bs, 2H), 3.42 (s, 3H), 3.15-3.12 (m, 1H), 2.45 (s, 3H), 1.34 (d, J=6.8 Hz, 6H).

Synthesis of 7-amino-3-isobutyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile. (1V)

Step-1: 2-Acetyl-4-methylpentanenitrile. (IVa)

To a three neck RB flask, LDA (2M in THF, 2.5 ml, 0.00514 mol) was taken and THF (10 ml) was added and cooled to −78° C. To this solution, 4-methylpentanenitrile (CAS:542-54-1, 0.5 g, 0.00514 mol) in THF (4 ml) was added dropwise at −78° C. and the reaction mixture was stirred at −78° C. for 1 h. Then, ethyl acetate (CAS:141-78-6, 0.45 ml, 0.00468 mol) was added dropwise at −78° C. and the reaction mixture was stirred at −78° C. for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with saturated solution of NH 4 Cl (5 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure to get the title compound IVa as yellow viscous oil; Yield: (0.478 g, 66%). 1 H NMR (400 MHz, CDCl 3 ): δ 3.46-3.42 (m, 1H), 2.40 (s, 3H), 1.89-1.76 (m, 2H), 1.70-1.65 (m, 1H), 1.0 (d, J=6.4 Hz, 3H), 0.96 (d, J=6.4 Hz, 3H).

›Abbreviations · 4 of 69

Step-2: 4-Isobutyl-5-methyl-1H-pyrazol-3-amine. (IVb)

To a stirred solution of 2-acetyl-4-methylpentanenitrile IVa (0.470 g, 0.00337 mol) in EtOH (20 ml) was added hydrazine hydrate (0.219 g, 0.00438 mol) and the reaction mixture was heated at 90° C. for 15 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to rt and evaporated under reduced pressure. The residue obtained was diluted with water (10 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure to get crude material IVb as light brown viscous liquid. The crude product obtained was taken as such for next step without further purification; Yield: (0.478 g, 92%). 1 H NMR (400 MHz, DMSO-D 6 ): δ 10.85 (bs, 1H), 4.13 (bs, 2H), 2.04 (d, J=7.2 Hz, 2H), 1.98 (s, 3H), 1.69-1.62 (m, 1H), 0.83 (d, J=6.8 Hz, 6H).

Step-3: 2-(Bis(methylthio) methylene) malononitrile. (Ia)

To a stirred solution of malononitrile (CAS:109-77-3, 5.0 g, 0.075 mol) in DMSO (50 ml) cooled to 0° C. was added K 2 CO 3 (11.4 g, 0.0833 mol), CS 2 (5.03 g, 0.0833 mol) and stirred at rt for 3 h. Then the reaction mixture cooled to 0° C. and methyl Iodide (9.4 ml, 0.1514 mol) was added and the reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was poured into ice cold water (50 ml) and the precipitate formed was filtered, washed with cold water and dried to get the compound Ia as brick red solid which was used in the next step without further purification; Yield: (6.2 g, 51%).

Step-4: 7-Amino-3-isobutyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile. (IVc)

To a stirred solution of 2-(bis(methylthio)methylene) malononitrile Ia (1.0 g, 0.0065 mol) in EtOH (10 ml) was added TEA (1.81 ml, 0.013 mol) and 4-isobutyl-5-methyl-1H-pyrazol-3-amine IVb (1.1 g, 0.0065 mol) and the reaction mixture was heated at 100° C. in microwave for 1 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated, and the residue was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure to get crude compound IVc. The crude product obtained was taken for next step without further purification; Yield: (1.64 g, 92%). LC_MS Calculated for C 13 H 17 N 5 S: 275.12; Observed: 276.20 [M + +H]. 1H NMR (400 MHz, DMSO-D 6 ): δ 8.51 (bs, 2H), 2.53-2.50 (m, 2H), 2.45 (d, J=7.2 Hz, 2H), 2.37 (t, J=3.6 Hz, 1H), 2.29 (s, 3H), 1.95-1.88 (m, 1H), 0.88-0.85 (m, 6H).

Step-5: 7-Amino-3-isobutyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (IV)

To a stirred solution of 7-amino-3-isobutyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile IVc (0.65 g, 0.00236 mol) in DCM (10 ml) cooled to 0° C., was added m-CPBA (1.0 g, 0.0059 mol) slowly and the reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC (polar spot). After completion, the reaction mixture was quenched with NaHCO 3 (50 ml) and extracted with DCM (50 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure to get the title compound IV. The crude product was used as such for the next step without further purification; Yield: (0.703 g, 97%). LC_MS Calculated for C 13 H 17 N 5 O 2 S: 307.11; Observed:308.15 [M + +H]. 1 H NMR (400 MHz, DMSO-D 6 ): δ 9.19 (bs, 2H), 3.40 (s, 3H), 2.53-2.52 (m, 2H), 2.41 (s, 3H), 1.95-1.92 (m, 1H), 0.96-0.87 (m, 6H).

Synthesis of 7-amino-3-(cyclopropyl methyl)-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile. (V)

Step-1: 2-cyclopropylethyl 4-methylbenzenesulfonate (Va)

To a stirred solution of 2-cyclopropylethan-1-ol 1 (CAS: 2566-44-1, 5.0 g, 0.0581 mol) in DCM (75 mL) cooled to 0° C. was added pyridine (12.6 mL) followed by an addition of p-TsCl (8.9 g, 0.0470 mol) and the reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with diethyl ether (100 ml) and washed sequentially with water (50 mL), 10% HCl (50 mL), water (10 ml) and dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure to get compound Va as colorless liquid. The crude product obtained was taken for the next step without further purification; Yield: (8.6 g, 66%). LC_MS Calculated for C 12 H 16 O 3 S: 140.32; Observed. 141.15 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 7.81 (d, J=8.4 Hz, 2H), 7.35 (d, J=8.4 Hz, 2H), 4.9 (t, J=6.4 Hz 2H), 2.46 (s, 3H), 1.56-1.51 (m, 2H), 0.67-0.65 (m, 1H), 0.40-0.39 (m, 2H), 0.04-0.01 (m, 2H).

Step-2: 3-Cyclopropylpropanenitrile (Vb)

To a stirred solution of 2-cyclopropylethyl 4-methylbenzenesulfonate Va (8.6 g, 0.0357 mol) in DMF (32 mL) was added TBAI (0.526 g) followed by an addition of sodium cyanide (5.2 g, 0.107 mol) and the reaction mixture was heated at 90° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with diethyl ether (150 mL) and washed with water (100 mL×3), brine (100 mL) and dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure to get desired product Vb as light brown viscous liquid. This crude product obtained was taken for the next step without further purification; Yield: (3.3 g, 97%). 1 H NMR (400 MHz, CDCl 3 ): δ 3.74-3.72 (m, 1H), 2.42 (t, J=6.8 Hz 2H), 1.59-1.51 (m, 2H), 0.86-0.81 (m, 1H), 0.56-0.53 (m, 2H), 0.16-0.13 (m, 2H).

Step-3: 2-(cyclopropyl methyl)-3-oxobutanenitrile (Vc)

A three neck RB flask was charged with LDA (2M sol in THF, 15.35 mL, 0.0315 mol) and THF (60 mL) under N 2 atmosphere. The resulting mixture was cooled to −78° C. and a solution of 3-cyclopropylpropanenitrile Vb (3.0 g, 0.0315 mol) in THF (24 mL) was dropwise added maintaining temperature to −78° C. The reaction mixture was stirred at −78° C. for 1 h and ethyl acetate (CAS: 141-78-6, 2.52 g, 0.0287 mol) was dropwise added at −78° C. The resulting mixture was stirred at −78° C. for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with saturated solution of NH 4 Cl (50 mL) and extracted with ethyl acetate (50 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure to get the title compound Vc as pale-yellow viscous oil; Yield: (3.25 g, 76%). 1 H NMR (400 MHz, CDCl 3 ): δ 3.50-3.47 (m, 1H), 2.41 (s, 3H), 1.92-1.82 (m, 1H), 1.80-1.75 (m, 1H), 0.9-0.86 (m, 1H), 0.61-0.55 (m, 2H), 0.25-0.23 (m, 1H), 0.18-0.14 (m, 1H).

›Abbreviations · 5 of 69

Step-4: 4-(cyclopropyl methyl)-5-methyl-1H-pyrazol-3-amine (Vd)

To a stirred solution of 2-(cyclopropyl methyl)-3-oxobutanenitrile Vc (3.2 g, 0.0233 mol) in EtOH (50 mL) was added hydrazine hydrate (2.19 ml, 0.0349) and the reaction mixture was heated at 90° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to rt and evaporated under reduced pressure. The residue obtained was diluted with NaHCO 3 solution (50 mL) and extracted with ethyl acetate (50 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure to get the title compound Vd. The crude product was taken for next step without further purification; Yield: (3.2 g, 91%). LC_MS Calculated for C 8 H 13 N 3 is 151.11; Observed. 152.20 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 3.80-3.60 (bs, 2H), 2.30 (d, J=5.6 Hz 3H), 1.70-1.50 (bs, 1H), 0.90-0.83 (m, 1H), 0.48-0.44 (m, 2H), 0.14-0.10 (m, 2H).

Step-5: 2-(Bis(methylthio) methylene) malononitrile (Ia)

Step-6: 7-amino-3-(cyclopropyl methyl)-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (Ve)

To a stirred solution of 2-(bis(methylthio)methylene) malononitrile Ia (3.3 g, 0.0198 mol) in pyridine (40 mL) was added 4-(cyclopropyl methyl)-5-methyl-1H-pyrazol-3-amine Vd (3.0 g, 0.0198 mol) and the reaction mixture was heated at 120° C. for 16 h under N 2 atmosphere. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to rt and poured into ice cold water (50 mL). The precipitate formed was filtered, dried, and washed with cold mixture of ethyl acetate (10%) and n-hexane (90%) and dried under vacuo to afford the desired compound as a pale-yellow solid; Yield: (4.3 g, 80%). LC_MS Calculated for C 13 H 15 N 5 S: 273.10; Observed: 274.10 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 6.26 (bs, 2H), 2.61-2.57 (m, 5H), 2.41 (s, 3H), 1.00-0.90 (bs, 1H), 0.45-0.43 (m, 2H), 0.24-0.22 (m, 2H).

Step-7:7-amino-3-(cyclopropyl methyl)-2-methyl-5-(methyl sulfonyl) pyrazolo [1,5-a] pyrimidine-6-carbonitrile. (V)

To a stirred solution of 7-amino-3-(cyclopropyl methyl)-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile Ve (2.3 g, 0.00842 mol) in DCM (50 mL) cooled to 0° C., was slowly added m-CPBA (5.8 g, 0.0337 mol) and the reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC. After completion, the reaction was quenched with NaHCO 3 (30 mL) and extracted with DCM (50 mL×3). The combined organic layer was again given the saturated NaHCO 3 (30 mL×2) wash followed by with brine. The organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under vacuo to afford the desired compound V as a pale-yellow solid; Yield: (2.1 g, 81%). LC_MS Calculated for C 13 H 15 N 5 O 2 S: 305.09; Observed:306.10 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 7.00-6.60 (bs, 2H), 3.39 (s, 3H), 2.64 (d, J=6.8, 2H), 2.50 (s, 3H), 0.99-0.97 (bs, 1H), 0.49-0.46 (m, 2H), 0.24-0.22 (m, 2H).

Synthesis of (6-methylpyridin-2-yl) methenamine. (VI)

Step-1: 6-methylpicolinaldehyde. (VIa)

To a solution of 2-bromo-6-methylpyridine (CAS: 5315-25-3, 5 g, 0.0290 mol) in Toluene (50 ml) was dropwise added n-BuLi (2.5M sol in THF, 11.62 ml, 0.0290 mol) at −78° C. Reaction mixture was stirred for 1 h at −78° C. To this solution, DMF (2.69 ml, 0.0348 mol) was dropwise added at −78° C. Reaction mixture was stirred for 1 h at −50° C. followed by stirring for 30 min at 5-10° C. and at rt for 40 min. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with saturated solution of NH 4 Cl (50 ml) and extracted with ethyl acetate (2×75 ml). The organic layer was washed with brine solution (50 ml) and dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure to get crude material which was column purified using 60-120 silica get. Product was eluted with 5% ethyl acetate in hexane to afford compound VIa as pale-yellow liquid. Yield: (1.4 g, 40%). LC-MS Calculated for C 7 H 7 NO: 121.15; Observe. 122.15 [M + +1]. 1 HNMR (400 MHz, CDCl 3 ): δ 10.054 (s, 1H), 7.745-7.799 (m, 2H), 7.386-7.404 (d, J=6.8 Hz, 1H), 2.672 (s, 3H).

Step-2: (6-methylpyridin-2-yl) methenamine. (VI)

To a stirred solution of 6-methylpicolinaldehyde VIa (1.4 g, 0.0115 mol) in Methanol (25 ml) was added Ammonium acetate (8.9 g, 0.115 mol) and the reaction mixture was stirred for 30 min at rt. Sodium cyanoborohydride (0.58 g, 0.0092 mol) was then portion wise added and continued further stirring at rt for 10 h. Progress of the reaction was monitored by TLC. After completion, solvent from reaction mass was removed under reduced pressure. The residue obtained was taken up with water (50 ml) and basified with aq. KOH. product was extracted with 5% MeOH in DCM (3×100 ml). Combined organic layer was dried over Na 2 SO 4 , filtered, and concentrated at reduced pressure to afford title compound VI as brown liquid. Yield: 1.2 g (85%). LC-MS Calculated for C 7 H 10 N 2 : 122.08; Observed. 123.08 [M + +1]. 1 HNMR (400 MHz, CDCl 3 ): δ 7.516-7.554 (t, J=7.6 Hz, 1H), 7.091-7.072 (d, J=7.6 Hz, 1H), 7.025-7.006 (d, J=7.6 Hz, 1H), 3.935 (s, 2H), 2.546 (s, 3H).

Synthesis of (6-ethylpyridin-2-yl) methenamine. (VII)

Step-1: 6-((trimethylsilyl)ethynyl) picolinonitrile. (VIIa)

›Abbreviations · 6 of 69

To a stirred solution of 6-bromopicolinonitrile (CAS: 122918-25-6, 1.5 g, 0.008 mol) in TEA (0.060 mol) was added and it was purged under nitrogen. To this Trimethylsilyl acetylene (1.37 ml, 0.009 mol) followed by copper iodide (0.036 g, 0.0003 mol) was added and purging was continued further for 10 more minutes. After that Pd (PPh 3 ) 2 Cl 2 (0.089 g, 0.0001 mol) was added. Then the reaction mixture was stirred at room temperature for 30 minutes. The progress of the reaction was monitored by TLC. After completion, reaction mixture was filtered over celite bed and concentrated to get desired crude product VIIa. Yield: (1.2 g 75%). LC_MS Calculated for C 11 H 12 N 2 Si: 200.08; Observed: 201.15 [M + +1]. 1 H NMR (400 MHz, CDCl 3 ): δ 7.824-7.784 (t, J=8.4 Hz, 1H), 7.652-7.617 (t, J=7.2 Hz, 2H), 0.287 (s, 9H).

Step-2: 6-ethynylpicolinonitrile. (VIIb)

To a stirred solution of 6-((trimethylsilyl)ethynyl) picolinonitrile VIIa (1.5 g, 0.005 mol) in MeOH (15 ml) cooled to 0° C., was added K 2 CO 3 (0.829 g, 0.005 mol) and the reaction mixture was stirred at 0° C. for 30 min. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with DCM (3×50 ml). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 20% ethyl acetate in hexane to yield the title compound VIIb as pale-yellow solid; Yield: (0.260 g, 34%). LC_MS Calculated for C 8 H 4 N 2 : 128.04; Observed.: Not ionized. 1 H NMR (400 MHz, CDCl 3 ): δ7.857-7.818 (d, J=7.6 Hz, 1H), 7.684-7.7.664 (d, J=8 Hz, 2H), 3.289 (s, H).

Step-3: (6-ethylpyridin-2-yl) methenamine. (VII)

To a stirred solution of 6-ethynylpicolinonitrile VIIb (0.260 g, 0.00203 mol) in MeOH (3 ml) purged with N 2 for 10 min, was added Pd/C (0.100 g) and the reaction mixture was stirred at rt for 24 h under H 2 atmosphere. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was filtered through celite and the filtrate was concentrated. The crude material was purified by column chromatography by eluting with 20% ethyl acetate in hexane to yield the title compound VII as pale-yellow liquid; Yield: (0.100 g, 36%). LC_MS Calculated for C 8 H 12 N 2 : 136.10; Observed.: 137 [M + +1]. 1 H NMR (400 MHz, CDCl 3 ): δ 7.583-7.544 (d, J=7.6 Hz, 1H), 7.094-7.075 (d, J=7.6 Hz, 1H), 7.040-7.021 (d, J=7.6 Hz, 1H), 3.959 (s, 2H), 2.840-2.784 (q, J=7.6 Hz, 2H), 1.322-1.255 (t, J=8 Hz, 3H).

Synthesis of N-methyl-1-(6-methylpyridin-2-yl) methenamine. (VIII)

Step-1: (E)-N-methyl-1-(6-methylpyridin-2-yl) methenamine. (VIIIa)

To a stirred solution of 6-methylpicolinaldehyde VIa (0.1 g, 0.000825 mol) in Methanol (5 ml) in molecular sieve was added methylamine (2 ml, 30% solution in methanol) and the resulting reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was filtered through the celite bed and evaporated under reduced pressure to get crude product VIIIa. The crude compound obtained was taken directly forward to the next step. Yield: (0.12 g, 100%).

Step-2: N-methyl-1-(6-methylpyridin-2-yl) methenamine. (VIII)

To a stirred solution of (E)-N-methyl-1-(6-methylpyridin-2-yl) methenamine VIIIa (0.120 g, 0.00089 mol) in methanol (5 ml), was added sodium borohydride (0.051 g, 0.00134 mol) at 0° C. Then the temp was allowed to reach room temperature and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure and diluted with 10 ml water and extracted with 10% methanol in DCM (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude purified by manual column by eluted with 10% methanol in DCM product was eluted at 10% methanol in DCM to get the product VIII. Yield: (0.123 g, 101%). LC_MS Calculated. for C 8 H 12 N 2 : 136.0; Observed:137 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 7.647-7.608 (t, J=8 Hz, 1H), 7.324-7.305 (d, J=7.6 Hz, 1H), 7.149-7.130 (d, J=7.6 Hz, 1H), 4.229 (s, 2H), 2.730 (s, 3H), 2.559 (s, 3H).

Synthesis of 1-(6-methylpyridin-2-yl) propan-2-amine. (IX)

Step-1: 1-(6-Methylpyridin-2-yl) propan-2-one. (IXa)

To a stirred solution of 2,6-dimethylpyridine (CAS: 108-48-5, 5.0 g, 0.046 mol) in THF (65 ml) at −78° C. was added n-BuLi (20.5 ml, 0.0512 mol) dropwise and stirred for 1 h. Then N,N-Dimethylacetamide (4.1 nil. 0.0443 mol) was added slowly at −78° C. and stirred at rt for 16 h. After completion (TLC), the reaction mixture was cooled to rt and quenched with 1.2 M HCl solution (25 ml) and stirred at rt for 30 min. Then, the reaction mixture was extracted with ethyl acetate (200 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by flash column chromatography by eluting with 5% ethyl acetate in hexane to yield the title compound IXa as yellow liquid; Yield: (0.713 g, 10.2%). 1 H NMR (400 MHz, CDCl 3 ): δ 7.54 (t, J=7.6 Hz, 1H), 7.05 (d, J=7.6 Hz, 1H), 7.00 (d, J=8.0, Hz, 1H), 3.89 (s, 2H), 2.54 (s, 3H), 2.22 (s, 3H).

Step-2: 1-(6-Methylpyridin-2-yl) propan-2-amine. (IX)

To a stirred solution of 1-(6-methylpyridin-2-yl) propan-2-one IXa (0.713 g, 0.00475 mol) in MeOH (20 ml), was added ammonium acetate (3.7 g, 0.04753 mol) and stirred at room temperature for 30 min. Then NaCNBH 3 (0.21 g, 0.003327 mol) was added portion-wise and the reaction mixture was stirred at room temperature for 16 h. Then the progress of the reaction was monitored by TLC. After completion, the reaction mixture was concentrated and diluted with saturated NaHCO 3 solution (50 ml) and extracted with diethyl ether (50 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure the desired product IX as pale green liquid. This crude product obtained was taken for the next step without further purification; Yield: (0.43 g, 60%). 1 H NMR (400 MHz, DMSO-d 6 ): δ 7.56 (t, J=7.6 Hz, 1H), 7.04 (d, J=7.6 Hz, 1H), 7.00 (d, J=7.6, Hz, 1H), 3.21-3.18 (m, 1H), 2.64 (dd, J=6.8 Hz, 2.4 Hz, 2H), 2.42 (s, 3H), 0.98 (dd, J=6.4 Hz, 2.4 Hz, 3H).

›Abbreviations · 7 of 69

Synthesis of 2-(6-(aminomethyl)pyridin-2-yl)propan-2-ol. (X)

Step-1: Pyridine-2,6-dicarboxylic acid. (Xa)

To a solution of 2,6-dimethylpyridine (CAS:108-48-5, 10 g, 0.17 mol) in 250 ml water, was slowly added 29.49 g of potassium permanganate (0.34 mol) with stirring. The mixture was refluxed until the purple color disappeared. Then, the mixture was cooled down to room temperature and another part of potassium permanganate (29.49 g, 0.34 mol) and water (150 ml) was slowly added. The mixture was refluxed at 100° C. over 16 hrs. until the purple color disappeared again. The mixture was cooled down to room temperature. Filtration and removal of solvent until the residual volume down to 100 ml, then sulfuric acid (70%, 17.5 ml) was added slowly. The precipitate was filtered to afford pyridine-2,6-dicarboxylic acid Xa. Yield: (8.5 g, 55%). LC_MS Calculated for C 7 H 5 NO 4 : 167.02; Observed.: 168.02 [M + +1].

Step-2: dimethyl pyridine-2,6-dicarboxylate. (Xb)

To a stirred solution of Pyridine-2,6-dicarboxylic acid Xa (8.5 g, 0.00598 mol) in methanol (1 L), added conc. sulphuric acid (4 ml) dropwise. The reaction mixture was refluxed at 60° C. for 16 hrs. After completion of reaction, removed the solvent by evaporation in vacuo, added water, the solid formed was filtered and dried to get the title compound Xb. Yield: (5 g, 51%). LC_MS Calculated for C 9 H 9 NO 4 is 195.05; Observed.: 196.15 [M + +1]. 1 H NMR (400 MHz, CDCl 3 ): δ 8.338-8.314 (m, 2H), 8.055-8.013 (m, 1H), 4.036 (s, 6H).

Step-3: methyl 6-(hydroxymethyl)picolinate. (Xc)

Into a 500-ml round-bottom flask, was placed a solution of 2,6-dimethyl pyridine-2,6-dicarboxylate Xb (5 g, 0.00487 mol) in a solvent mixture of methanol (174 ml) and dichloromethane (74 ml). NaBH 4 (1.45 g, 0.00502 mol) was added to the reaction mixture in portions at 0° C. The resulting solution was stirred overnight at room temperature, and then it was quenched by the addition of Aq. NH 4 Cl (250 ml). The resulting solution was extracted with dichloromethane (2×200 ml) and the combined organic layers were dried over Na 2 SO 4 , filtered, and concentrated under vacuum. The residue was applied onto a silica gel column with ethyl acetate/petroleum ether (1:1) as eluent to yield methyl 6-(hydroxymethyl) pyridine-2-carboxylate Xc as white solid. Yield: (3.9 g, 93%). LC_MS Calculated. for C 8 H 9 NO 3 is 167.16; Observed.: 168.20 [M + +1]. 1 H NMR (400 MHz, CDCl 3 ): δ 8.048-8.029 (d, J=7.6 Hz, 1H), 7.875-7.837 (t, J=7.6 Hz, 1H), 7.541-7.522 (d, J=7.6 Hz, 1H), 4.863 (s, 2H), 4.002 (s, 3H).

Step-4: 2-(6-(hydroxymethyl) pyridin-2-yl) propan-2-ol. (Xd)

To a stirred solution of methyl 6-(hydroxymethyl) picolinate Xc (3 g, 0.0179 mol) in diethyl ether (300 ml), added methyl magnesium bromide (3.4 M, 0.0358 mol, 10.5 ml) dropwise at 0° C. Then the reaction mixture was stirred at room temperature for 3 hrs. After completion, the reaction mixture was quenched with NH 4 Cl and extracted with ethyl acetate (3×200 ml). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 30% ethyl acetate in hexane to yield the title compound as pale-yellow oil; Yield: (2.2 g, 73%). LC_MS Calc. for C 8 H 9 NO 3 is 167.16; Obs.: 168.20 [M + +1]. 1 H NMR (400 MHz, CDCl 3 ): δ 7.735-7.696 (t, J=8 Hz, 1H), 7.333-7.313 (d, J=8 Hz, 1H), 7.201-7.182 (d, J=7.6 Hz, 1H), 4.783 (s, 2H), 4.412 (s, 1H), 3.190 (s, 1H), 1.566 (s, 6H).

Step-5: 2-(6-(azidomethyl) pyridin-2-yl) propan-2-ol. (Xe)

To a stirred solution of 2-(6-(hydroxymethyl) pyridin-2-yl) propan-2-ol Xd (0.4 g, 0.0832 mol) in THF (5 ml), DPPA (0.0832 mol) at room temperature. The mixture was cooled to 0° C. and DBU (0.0832 mol) was added at rate such that the internal temperature did not exceed 20° C. The reaction mixture was then warmed to 40° C. and stirred for 16 hrs. After completion, the reaction mixture was cooled to room temperature, added water (20 ml) and extracted with ethyl acetate (3×20 ml). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 10% ethyl acetate in hexane to yield the title compound Xe as brown liquid; Yield: (0.26 g, 57%). LC_MS Calculated for C 9 H 2 N 4 O is 192.10; Observed.: 193.10 [M + +1]. 1 H NMR (400 MHz, CDCl 3 ): δ 7.743-7.724 (t, J=7.6 Hz, 1H), 7.337-7.317 (d, J=8 Hz, 1H), 7.245-7.226 (d, J=7.6 Hz, 1H), 4.905 (s, 1H), 4.465 (s, 2H), 1.550 (s, 6H).

Step-6: 2-(6-(aminomethyl) pyridin-2-yl) propan-2-ol. (X)

To a stirred solution of 2-(6-(azidomethyl) pyridin-2-yl) propan-2-ol Xe (0.26 g) in methanol (10 ml), added Pd/C (100 mg) at room temperature. The reaction was stirred at same temperature for 1 hr under hydrogen. After completion, the reaction mixture was filtered through celite bed, and the bed was washed with methanol. The filtrate was concentrated under reduced pressure to get the title compound X as brown liquid; Yield: (0.3 g, crude). The crude product obtained was taken for the next step without further purification.

Synthesis of 2-(5-methylpyridin-2-yl)ethan-1-amine. (XI)

Step-1: 2-(5-Methylpyridin-2-yl) acetonitrile. (XIa)

To a stirred solution of n-BuLi (14 ml, 0.034 mol) in THF (75 ml) was added ACN (1.75 ml, 0.0388 mol) at −78° C. and stirred for 1 h. Then added 2-bromo-5-methylpyridine (CAS: 3510-66-5, 1.67 g, 0.0097 mol) slowly and stirred at rt for 2 h. After completion (TLC), the reaction mixture was diluted with ice cold water (50 ml) and extracted with ethyl acetate (50 ml×3). The organic layer was dried over Na 2 SO 4 , filtered, and evaporated under reduced pressure to get the desired product Xia Yield: (0.6 g, crude). The crude product was taken for the next step without further purification.

Step-2: 2-(5-Methylpyridin-2-yl) ethan-1-amine. (XI)

To a stirred solution of 2-(5-methylpyridin-2-yl) acetonitrile XIa (0.6 g, 0.0045 mol) in THF (100 ml) was added BH 3 -Me 2 S, (10 ml, 0.0020 mol) at room temperature and stirred for 2 h at 70° C. After completion (TLC), the reaction mixture cooled to room temperature, then added MeOH (5 ml) and 10 ml 1N HCl and the aqueous layer washed with ethyl acetate separated the layer and the aqueous layer basified with 1N NaOH and extracted with DCM (100 ml×3). The organic layer was dried with Na 2 SO 4 , filtered and evaporated under reduced pressure to give title compound XI, the crude product was taken for the next step without purification; Yield: (0.2 g, 32%). LC_MS Calculated for C 8 H 12 N 2 is 136.10; Observed. 137.2 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 8.35 (s, 1H), 7.42 (d, J=7.6 Hz 1H), 7.06 (d, J=8.0 Hz 1H), 3.12 (t, J=6.4 Hz, 2H), 2.91 (d, J=6.4 Hz, 2H), 2.3 (s, 3H).

›Abbreviations · 8 of 69

Synthesis of 2-(5-fluoropyridin-2-yl)ethan-1-amine. (XII)

Step-1: 5-Fluoropicolinaldehyde. (XIIa)

To a stirred solution of 2-bromo-5-fluoropyridine (CAS: 41404-58-4, 2.69 g, 0.0147 mol) in toluene (30 ml) at −78° C. was added n-BuLi (5.9 ml, 0.0147 mol) dropwise and stirred for 1 h. Then DMF (1.36 ml) and added slowly at −50° C. and stirred for 30 min. After completion (TLC), the reaction mixture was quenched with MeOH (30 ml) and stirred at 5-10° C. for 30 min. Then, NH 4 Cl solution (75 ml) was added and stirred at rt for 40 min and then extracted with ethyl acetate (100 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by flash column chromatography by eluting with 5% ethyl acetate in hexane to yield the title compound XIIa as yellow liquid; Yield: (1.01 g, 54.89%). LC_MS Calculated for C 6 H 4 FNO is 125.03; Observed. 126.00 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 10.04 (s, 1H), 8.63 (d, J=2.0 Hz, 1H), 8.05-8.02 (m, 1H), 7.58 (dt, J=8.4 Hz, 2.8 Hz, 1H).

Step-2: (E)-5-Fluoro-2-(2-nitrovinyl) pyridine. (XIIb)

To a stirred solution of 5-fluoropicolinaldehyde XIIa (1.01 g, 0.00807 mol) in DCM (20 ml), was added TEA (2.24 ml, 0.0161 mol) and nitromethane (0.52 ml, 0.00968 mol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. The crude material was dissolved in DCM (20 ml) and treated with TEA (1.77 ml, 0.0242 mol) and MsCl (2.75 ml, 0.0242 mol) at 0° C. and the reaction mixture was stirred at rt for 20 min. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. And the crude was purified by column chromatography by eluting with 10% ethyl acetate in hexane to yield the title compound XIIb as green solid; Yield: (1.2 g, 88.8%). LC_MS Calculated for C 7 H 5 FN 2 O 2 is 168.03; Observed. 169.00 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 8.54 (s, 1H), 7.93 (q, J=12.8 Hz, 2H), 7.50-7.49 (m, 2H).

Step-3: 2-(5-Fluoropyridin-2-yl) ethan-1-amine. (XII)

To a stirred solution of (E)-5-fluoro-2-(2-nitrovinyl) pyridine XIIb (1.2 g, 0.0071 mol) in THF (50 ml), was added LAH (1M sol in THF, 21.4 ml, 0.024 mol) at −20° C. dropwise. Then the temp was allowed to reach to rt, and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with saturated Na 2 SO 4 solution (50 ml) and extracted with ethyl acetate (50 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude was purified by column chromatography by eluting with 10% methanolic NH 3 in DCM to yield the title compound as pale green liquid; Yield: (0.35 g, 35%). LC_MS Calculated for C 7 H 9 FN 2 is 140.07; Observed. 141.2 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.45 (d, J=3.2 Hz, 1H), 7.62 (dd, J=8.4 Hz, 2.8 Hz, 1H), 7.34-7.31 (m, 1H), 2.88 (t, J=6.4 Hz, 2H), 2.81 (d, J=6.4 Hz, 2H), 2.5 (bs, 2H).

Synthesis of 2-(6-methoxypyridin-2-yl) ethan-1-amine. (XIII)

Step-1: (E)-6-Methoxy-2-(2-nitrovinyl) pyridine. (XIIIa)

To a stirred solution of 6-methoxypicolinaldehyde (CAS:54221-96-4, 1.0 g, 0.00724 mol) in DCM (20 ml), was added TEA (2.19 g, 0.0217 mol) and nitromethane (1.32 g, 0.0216 mol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. The crude material was dissolved in DCM (20 ml) and treated with TEA (2.19 g, 0.0217 mol) and mesyl chloride (2.47 g, 0.0217 mol) at 0° C. and the reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. And the crude was purified by column chromatography by eluting with 7% ethyl acetate in hexane to yield the title compound XIIa as yellow solid; Yield: (0.8 g, 80%). 1 H NMR (400 MHz, CDCl 3 ): δ8.016-7.983 (d, J=13.2 Hz, 1H), 7.845-7.812 (d, J=13.2 Hz, 1H), 7.652-7.614 (t, J=8 Hz, 1H), 7.069-7.052 (d, J=6.8 Hz, 1H), 6.863-6.843 (d, J=8 Hz, 1H), 3.969 (s, 3H).

Step-2: 2-(6-Methoxypyridin-2-yl) ethan-1-amine. (XIII)

To a stirred solution of (E)-6-methoxy-2-(2-nitrovinyl) pyridine 2 (0.8 g, 0.0044 mol) in DEE (16 ml), was added LAH (1M sol in THF, 13.3 ml, 0.0133 mol) at −10° C. dropwise. Then the temp was allowed to reach to rt and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with chilled water (10 ml), 15N aq. NaOH (1 ml), and extracted with 10% methanol in DCM (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure to give crude product XIII as brown liquid. The crude product was carried forward to next step without purification; Yield: (0.43 g, 63%). LC_MS Calculated for C 8 H 12 N 2 O: 152; Observe. 153 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 7.477 (d, 1H), 6.733-6.726 (d, J=2.8 Hz, 1H), 6.570-6.560 (m, 2H), 3.918 (s, 3H), 3.108 (t, 2H), 2.820 (t, 2H).

Synthesis of 2-(6-methylpyridin-2-yl) ethan-1-amine. (XIV)

Step-1. (E)-6-Methoxy-2-(2-nitrovinyl) pyridine. (XVa)

To a stirred solution of 6-methylpicolinaldehyde Via (8.0 g, 0.0660 mol) in DCM (80 ml), was added TEA (13.36 g, 0.132 mol) and nitromethane (5.15 g, 0.079 mol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. The crude material was dissolved in DCM (80 ml) and treated with TEA (20.03 g, 0.198 mol) and mesyl chloride (22.58 g, 0.198 mol) at 0° C. and the reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. And the crude was purified by column chromatography by eluting with 7-10% ethyl acetate in hexane to yield the title compound XVa as brown liquid; Yield: (9 g, 83.33%). 1 H NMR (400 MHz, CDCl 3 ): δ 8.076-8.042 (d, J=13.6 Hz, 1H), 7.942-7.910 (d, J=12.8 Hz, 1H), 7.722-7.605 (t, J=7.2 Hz, 1H), 7.331-7.314 (d, J=6.8 Hz, 1H), 7.278-7.7.266 (d, J=4.8 Hz, 1H), 2.263 (s, 3H).

›Abbreviations · 9 of 69

Step-3: 2-(6-methylpyridin-2-yl) ethan-1-amine (XIV)

To a stirred solution of (E)-6-methoxy-2-(2-nitrovinyl) pyridine 2 (9.18 g, 0.0559 mol) in DEE (700 ml), was added LAH (1M sol in THF, 224 ml, 0.22 mol) at 0° C. dropwise. Then the temp was raised to 40° C. and continued for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with chilled water (50 ml), 15N aq. NaOH (5 ml), and filtered through celite bed. Celite bed was washed with DEE (250 ml). DEE from filtrate was distilled off at reduced pressure to get title compound XIV brown liquid which was used for next step without further purification. Yield: (5.09 g, 67%). LC_MS Calculated. for C 18 H 12 N 2 : 136; Observed. 137 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 7.577-7.539 (t, J=8 Hz, 1H), 7.050-7.012 (m, 2H), 2.867-2.807 (t, J=6.8 Hz, 2H), 2.771-2.750 (t, J=8.4 Hz, 2H), 2.420 (s, 3H).

Synthesis of 2-(6-(2-aminoethyl) pyridin-2-yl)propan-2-ol. (XV)

Step-1: 6-(2-hydroxypropan-2-yl) picolinaldehyde (XVa)

To a stirred solution of 2-(6-(hydroxymethyl) pyridin-2-yl) propan-2-ol Xd (1.8 g, 0.0107 mol) in DCM (50 ml), added DMP (6.85 g, 0.0161 mol) portion wise at 0° C. And the reaction mixture was stirred at same temperature for 30 min. The reaction was monitored by TLC. After completion, the reaction mixture was quenched with aq. Sodium bicarbonate (50 ml) and extracted by DCM (100 ml×3 times). The organic layer was dried over sodium sulfate, filtered, and concentrated under reduced pressure. Crude compound was purified by column chromatography, the product was eluted at 10% ethyl acetate/Hexane to afford title compound XVa as pale-yellow oil. Yield: (1.1 g, 62%). 1 H NMR (400 MHz, CDCl 3 ): δ 10.094 (s, 1H), 7.929-7.870 (m, 2H), 7.659-7.641 (d, J=7.2 Hz, 1H), 4.606 (bs, 1H), 1.607 (s, 6H).

Step-2: (E)-2-(6-(2-nitrovinyl) pyridin-2-yl) propan-2-ol (XVb)

To a stirred solution of 6-(2-hydroxypropan-2-yl) picolinaldehyde XVa (0.5 g, 0.00303 mol) in DCM (5 ml), was added TEA (0.613 g, 0.00606 mol) and nitromethane (0.1 g, 0.0016 mol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. The crude material was dissolved in DCM (20 ml) and treated with TEA (0.67 g, 0.00663 mol) and mesyl chloride (0.756 g, 0.00663 mol) at 0° C. and the reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. And the crude was purified by column chromatography by eluting with 7% ethyl acetate in hexane to yield the title compound XVb as yellow solid; Yield: (0.35 g, 76%). LC_MS Calculated for C 10 H 12 N 2 O 3 : 208.08; Observed:209.20 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 8.036-8.003 (d, J=13.2 Hz, 1H), 7.951-7.918 (d, J=13.2 Hz, 1H), 7.841-7.801 (t, J=8 Hz, 1H), 7.531-7.510 (d, J=8.4 Hz, 1H), 7.406-7.388 (d, J=7.2 Hz, 1H), 1.577 (s, 6H).

Step-3: 2-(6-(2-aminoethyl) pyridin-2-yl) propan-2-ol (XV)

To a stirred solution of (E)-1-Methyl-3-(2-nitrovinyl)-1H-pyrazole 3 (0.55 g, 0.00264 mol) in diethyl ether (26 ml), was added LAH (1M solution in THF, 0.401 g, 0.0106 mol) at 0° C. dropwise. Then the temp was allowed to reach to rt and the reaction mixture was stirred at 40° C. for 3 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with chilled water (3 ml), 15N aq. NaOH (2 ml). Inorganic salts were filtered and washed with DEE (25 ml). Solvent from filtrate were concentrated under reduced pressure to afford crude compound XV which was used for next step without further purification. Yield: (0.3 g, 63%). 1H NMR (400 MHz, DMSO-d 6 ): δ 7.666-7.628 (t, J=7.2 Hz, 1H), 7.450-7.432 (d, J=7.2 Hz, 1H), 7.069-7.050 (d, J=7.6 Hz, 1H), 2.886-2.870 (t, J=6.4 Hz, 2H), 2.784-2.766 (t, J=7.2 Hz, 2H), 1.414 (s, 6H).

Synthesis of 2-(1-ethyl-1H-pyrazol-3-yl) ethan-1-amine. (XVI)

Step-1: 1-ethyl-1H-pyrazole-3-carbaldehyde (XVIa)

To a stirred solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1, 3 g, 0.031 mol) in DMF (20 ml) was added Potassium carbonate (10 g, 0.0723 mol) and Ethyl iodide (5.8 g, 0.0374 mol) and the reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC. After completion, water (30 ml) was added and extracted with ethyl acetate (3×30 ml). Combined ethyl acetate layer was washed with 10% brine solution (50 ml). Organic layer was dried over sodium sulphate, filtered, and concentrated to afford crudel-ethyl-1H-pyrazole-3-carbaldehyde which was further purified by column chromatography using 30% ethyl acetate in hexane to afford title compound XVI as yellow colored liquid. Yield: (2.4 g, 63%). 1 H NMR (400 MHz, CDCl 3 ): δ 9.982 (s, 1H), 7.467-7.462 (d, J=2 Hz, 1H), 6.808-6.802 (d, J=2.4 Hz, 1H), 4.307-4.252 (q, J=7.2 Hz, 2H), 1.572-1.536 (t, J=7.2 Hz, 3H).

Step-2: (E)-1-ethyl-3-(2-nitroyinyl)-1H-pyrazole (XVIb)

To a stirred solution of 1-ethyl-1H-pyrazole-3-carbaldehyde 2 (2.4 g, 0.0193 mol) in nitromethane (22 ml) was added Ammonium acetate (2.3 g, 0.029 mol) and the reaction mixture was stirred for 1 h at 100° C. Progress of reaction was monitored by TLC. After completion, solvent from reaction mass was removed under reduced pressure. Residue was taken up with water (50 ml) and extracted with ethyl acetate (3×35 ml). Combined ethyl acetate layer was washed with 10% brine solution (35 ml). Organic layer was dried over Na 2 SO 4 , filtered, and concentrated under reduced pressure to get the desired product XVIb. The crude product was carried forward to next step without further purification. Yield: (1.6 g, 49%). 1 H NMR (400 MHz, CDCl 3 ): δ 7.990-7.956 (d, J=13.6 Hz, 1H), 7.637-7.604 (d, J=13.2 Hz, 1H), 7.450-7.446 (d, J=1.6 Hz, 1H), 6.542-6.538 (d, J=1.6 Hz, 1H), 4.253-4.190 (q, J=7.2 Hz, 2H), 1.543-1.506 (t, J=7.2 Hz, 3H).

Step-3: 2-(1-ethyl-1H-pyrazol-3-yl) ethan-1-amine (XVI)

›Abbreviations · 10 of 69

To a stirred solution of (E)-1-ethyl-3-(2-nitrovinyl)-1H-pyrazole 3 (1.5 g, 0.0089 mol) in DEE (60 ml), was added LAH (1M sol in THF, 36 ml, 0.036 mol) at 0° C. dropwise. Then the temp was allowed to reach to rt and the reaction mixture was stirred at 40° C. for 3 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with chilled water (2 ml), 15N aq. NaOH (1 ml). Inorganic salts were filtered and washed with DEE (25 ml). Solvent from filtrate were concentrated under reduced pressure to afford crude compound XVI which was used for next step without further purification. Yield: (1 g, 81%). 1 H NMR (400 MHz, CDCl 3 ): δ 7.312 (d, 1H), 6.054-6.050 (d, 1H), 4.150-4.096 (q, J=7.2 Hz, 2H), 3.008-2.974 (t, 2H), 2.787-2.754 (t, 2H), 1.483-1.432 (t, J=7.2 Hz, 3H).

Synthesis of 2-(1-ethyl-1H-pyrazol-3-yl)ethan-1-amine. (XVII)

Step-1: 1-methyl-1H-pyrazole-3-carbaldehyde (XVIIa)

To a stirred solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1.5 g, 0.052 mol) in DMF (25 ml) was added Potassium carbonate (17.97 g, 0.13 mol) and Methyl iodide (8.86 g, 0.0624 mol) and the reaction mixture was stirred at room temperature for 16 h. The progress of the reaction was monitored by TLC. After completion, water (50 ml) was added and extracted with ethyl acetate (3×50 ml). Combined ethyl acetate layer was washed with 10% brine solution (50 ml). Organic layer was dried over sodium sulphate, filtered, and concentrated to afford crude 1-Methyl-1H-pyrazole-3-carbaldehyde, which was further purified by column chromatography using 30% ethyl acetate in hexane to afford title compound XVIIa as yellow colored liquid. Yield: (2.6 g, 45%). 1 H NMR (400 MHz, CDCl 3 ): δ 9.960 (s, 1H), 7.431-7.427 (d, J=1.6 Hz, 1H), 6.812-6.805 (d, J=2.8 Hz, 1H), 4.020 (s, 1H).

Step-2: (E)-1-methyl-3-(2-nitrovinyl)-1H-pyrazole (XVIIb)

To a stirred solution of 1-Methyl-1H-pyrazole-3-carbaldehyde XVIIa (2.4 g, 0.020 mol) in nitromethane (43 ml) was added ammonium acetate (2.5 g, 0.031 mol) and the reaction mixture was stirred for 1 h at 100° C. Then the progress of reaction was monitored by TLC. After completion, solvent from reaction mass was removed under reduced pressure. Residue was taken up with water (50 ml) and extracted with ethyl acetate (3×50 ml). Combined ethyl acetate layer was washed with 10% brine solution (50 ml). Organic layer was dried over Na 2 SO 4 , filtered, and concentrated under reduced pressure. Crude product was purified by column chromatography using 20% ethyl acetate in hexane to afford pure title compound XVIIb. Yield: (1.4 g, 49%). 1 H NMR (400 MHz, CDCl 3 ): δ7.970-7.936 (d, J=13.6 Hz, 1H), 7.631-7.598 (d, J=13.2 Hz, 1H), 7.428-7.422 (d, J=2.4 Hz, 1H), 6.549-6.543 (d, J=2.4 Hz, 1H), 3.971 (s, 3H).

Step-3: 2-(1-Methyl-1H-pyrazol-3-yl) ethan-1-amine (XVII)

To a stirred solution of (E)-1-Methyl-3-(2-nitrovinyl)-1H-pyrazole 3 (1.4 g, 0.009 mol) in DEE (60 ml), was added LAH (1M solution in THF, 29 ml, 0.029 mol) at 0° C. dropwise. Then the temp was allowed to reach to rt and the reaction mixture was stirred at 40° C. for 3 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with chilled water (2 ml), 15N aq. NaOH (1 ml). Inorganic salts were filtered and washed with DEE (25 ml). Solvent from filtrate were concentrated under reduced pressure to afford crude compound XVII which was used for next step without further purification. Yield: (1 g, 87%). 1 H NMR (400 MHz, CDCl 3 ): δ 7.278-7.268 (d, J=4 Hz, 1H), 6.053-6.050 (d, J=1.2 Hz, H), 3.853 (s, 3H), 3.008-2.969 (t, J=6.8 Hz, 2H), 2.778-2.745 (t, J=6.8 Hz, 2H).

Synthesis of 2-(3-(2-aminoethyl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol. XVIII

Step-1: Ethyl 2-(3-formyl-1H-pyrazol-1-yl)-2-methylpropanoate (XVIIIa)

To a stirred solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1, 1 g, 0.01 mol) in DMF (5 ml) was added Potassium carbonate (3 g, 0.02 mol) and ethyl 2-bromo-2-methylpropanoate (2 g, 0.01 mol) and the reaction mixture was stirred at room temperature for 16 h. Then the progress of the reaction was monitored by TLC. After completion, water (25 ml) was added and extracted with ethyl acetate (3×25 ml). Combined ethyl acetate layer was washed with 10% brine solution (25 ml). Organic layer was dried over sodium sulphate, filtered, and concentrated to afford crud compound which was further purified by column chromatography using 30% ethyl acetate in hexane to afford title compound XVIIIa. Yield: (1.8 g, 82%). LC_MS Calculated for C 10 H 14 N 2 O 3 is 210.06; Observed.: 211.20 [M + +1]. 1 H NMR (400 MHz, CDCl 3 ): δ 9.980 (s, 1H), 7.623 (s, 1H), 6.836 (s, 1H), 4.217-4.164 (q, J=7.2 Hz, 2H), 1.999 (s, 6H), 1.235-1.200 (t, J=6.8 Hz, 3H).

Step-2: ethyl (E)-2-methyl-2-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) propanoate (XVIIIb)

To a stirred solution of ethyl 2-(3-formyl-1H-pyrazol-1-yl)-2-methylpropanoate XVIIIa (1.8 g, 0.008 mol) in nitromethane (17 ml) was added Ammonium acetate (0.99 g, 0.0121 mol) and the reaction mixture was stirred for 1 h at 100° C. Progress of reaction was monitored by TLC. After completion, solvent from reaction mass was removed under reduced pressure. Crude product was purified by column chromatography using 20% ethyl acetate in hexane to afford pure title compound XVIIIb. Yield: (0.55 g, 25%). LC_MS Calculated for C 11 H 15 N 3 O 4 is 253.11; Observed: 254.20 [M + +1]. In NMR (400 MHz, CDCl 3 ): δ 8.007-7.973 (d, J=13.6 Hz, 1H),7.629-7.611 (m, 2H), 7.509-7.504 (d, J=2 Hz, 1H), 6.580-6.574 (d, J=2.4 Hz, 1H), 4.210-4.120 (q, J=7.6 Hz, 2H), 1.187 (s, 6H), 1.237-1.163 (t, J=7.6 Hz, 3H).

Step-3: 2-(3-(2-aminoethyl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol (XVIII)

To a stirred solution of ethyl (E)-2-methyl-2-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) propanoate 4 (0.55 g, 0.0021 mol) in DEE (15 ml), was added LAH (1M solution in THF, 8.7 ml, 0.0086 mol) at 0° C. dropwise. Then the temp was allowed to reach to rt and the reaction mixture was stirred at 40° C. for 3 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with chilled water (2 ml), 15N aq. NaOH (1 ml). Inorganic salts were filtered and washed with DEE (25 ml). Solvent from filtrate were concentrated under reduced pressure to afford crude compound XVIII which was used for next step without further purification. Yield: (0.35 g, 88%). LC_MS Calculate for C 9 H 17 N 3 O is 183.14; Observe: 184.30 [M + +1]. In NMR (400 MHz, CDCl 3 ): δ 7.449 (s, 2H), 6.071 (s, 1H), 3.76 (s, 2H), 2.999-2.968 (t, J=7.6 Hz, 2H), 2.811-2.740 (t, J=7.6 Hz, 2H), 1.596 (s, 6H).

›Abbreviations · 11 of 69

Synthesis of 2-(5-methoxypyridin-2-yl)ethan-1-amine. XIX

Step-1: (E)-5-Methoxy-2-(2-nitrovinyl) pyridine (XIXa)

To a stirred solution of 5-methoxypicolinaldehyde 1 (2.0 g, 0.0145 mol) in DCM (20 ml), was added TEA (4.04 ml, 0.029 mol) and nitromethane 2 (1.06 g, 0.0174 mol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. The crude material was dissolved in DCM (20 ml) and treated with TEA (6.06 ml, 0.0435 mol) and mesyl chloride (3.37 ml, 0.0435 mol) at 0° C. and the reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure. The crude was purified by column chromatography by eluting with 10% ethyl acetate in hexane to yield the title compound XIXa as yellow solid; Yield: (2.47 g, 94%). LC_MS Calculated for C 8 H 8 N 2 O 3 : 180.05; Observe: 181.00 [M + +H]. 1 H NMR (400 MHz, CDCl 3 ): δ 8.38 (d, J=3.2 Hz, 1H), 7.43 (d, J=8.4 Hz, 1H), 7.26 (s, 1H), 7.22 (dd, J=8.4, 2.8 Hz, 1H), 3.92 (s, 3H).

Step-2: 2-(5-Methoxypyridin-2-yl) ethan-1-amine (XIX)

To a stirred solution of (E)-5-methoxy-2-(2-nitrovinyl) pyridine 3 (2.74 g, 0.0152 mol) in THF (90 ml), was added LAH (1M sol in THF, 45.6 ml, 0.0456 mol) at −10° C. dropwise. Then the temp was allowed to reach to rt and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to 0° C. and quenched with saturated Na 2 SO 4 solution (50 ml) and extracted with ethyl acetate (50 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude was purified by column chromatography by eluting with 10% methanolic NH 3 in DCM to yield the title compound XIX as brown liquid; Yield: (0.8 g, 34.78%). 1 H NMR (400 MHz, DMSO): δ 8.19 (d, J=2.8 Hz, 1H), 7.3 (dd, J=8.4, 3.2 Hz, 1H), 7.19 (d, J=8.8 Hz, 1H), 3.79 (s, 3H), 2.88 (t, J=6.0 Hz, 2H), 2.74 (t, J=6.8 Hz, 2H).

Synthesis of a mixture of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl)pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XX) and 7-amino-3-chloro-2-methyl-5-(methyl sulfinyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile. (XXa)

Step-1: Synthesis of 4-chloro-3-methyl-1H-pyrazol-5-amine (XXb)

N-Chlorosuccinimide (3.3 g, 0.025 mol) was added portion wise to the stirred solution of 3-methyl-1H-pyrazol-5-amine (CAS: 268724-49-8, 2.0 g, 0.021 mol) in DCM (50 mL) at 0° C., Then the reaction mixture was stirred at rt for 1 h. The progress of the reaction was monitored by TLC. Then the reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (3×50 mL). The combined organic layer was dried over anhydrous Na 2 SO 4 and evaporated under reduced pressure to get the crude product. Crude compound obtained was purified by Biotage column chromatography using silica gel (230-400) and 1-5% methanol in DCM as eluent to afford desired product XXb as an off white solid. Yield: 2.0 g, 74%. LCMS Calculated. for C 4 H 6 ClN 3 is 131.56; Observed. 132.0 [M+H] +

Step-2: Synthesis of 7-amino-3-chloro-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXc)

To a stirred solution of 4-chloro-5-methyl-1H-pyrazol-3-amine XXb (1.8 g, 0.0136 mol) in pyridine (5 mL) was added 2-(bis (methylthio) methylene) malononitrile Ia (2.8 g, 0.016 mol). The resulting reaction mixture was heated at 120° C. for 2.5 h. Then the progress of the reaction was monitored by TLC. Reaction mixture was cooled to room temperature and poured into ice cold water (20 mL). Precipitated compound was filtered and washed with cold water. Then the obtained solid was dried under vacuum and purified by biotage column chromatography using silica gel (230-400) and 5-20% ethyl acetate in hexane as eluent to afford desired product XXc as pale brown colour solid. Yield: 3.5 g, 99%. LCMS Calculated. for C 9 H 8 ClN 5 S is 253.71; Observed. 254.0 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.90 (bs, 2H), 2.57 (S, 3H), 2.37 (S, 3H).

Step-3: Synthesis of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile. (XX) and 7-amino-3-chloro-2-methyl-5-(methyl sulfinyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile (XXa)

meta-Chloroperoxybenzoic acid (9.5 g, 0.055 mol) was added portion wise to a stirred solution of 7-amino-3-chloro-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXc (3.5 g, 0.013 mol) in DCM (50 mL) at 0° C. The reaction mixture was stirred at rt for 3 h. Then the progress of the reaction was monitored by TLC. The reaction mixture was quenched with aqueous NaHCO 3 solution (50 mL) and extracted with DCM (3×50 mL). The combined organic layer was dried over anhydrous Na 2 SO 4 and evaporated under reduced pressure and dried under vacuum to get a mixture of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX and 7-amino-3-chloro-2-methyl-5-(methyl sulfinyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXa as an off white solid. Yield (2.2 g, 56%). This crude material was taken as such for next step without further purification. LCMS Calculated. for C 9 H 8 ClN 5 O 2 S is 285.71; Observed. 286.1 [M+H] 1 H NMR (400 MHz, DMSO-D6): δ 9.48 (bs, 2H), 3.42 (S, 3H), 2.45 (S, 3H).

Synthesis of 2-(6-(methoxymethyl) pyridin-2-yl) ethan-1-amine (XXI)

Step-1: Synthesis of methyl 6-methoxy methyl) picolinate. (XXIa)

A solution of methyl 6-(hydroxymethyl) picolinate (CAS: 1197-10-0, 25 g, 0.15 mol) in DMF (250 mL) was cooled to 0° C., added NaH (7.2 g, 60% wt, 0.18 mol) portion wise and dimethyl sulphate (19 mL, 0.19 mol) dropwise to it and the reaction mixture was stirred at rt for 3 hours. Then the progress of the reaction was monitored by TLC. After completion, the reaction mixture was quenched with ice cold water (200 mL) and extracted with ethyl acetate (3×200 mL) and concentrated. Crude product obtained was purified by gravity column used 230-400 silica mesh and the desired product was eluted at 15-20% ethyl acetate in hexane to afford XXIa as yellow viscous oil. Yield: 21 g, 78%. LCMS Calculated. for C 9 H 11 NO 3 is 181.07; Observed 182.20 [M+H]. 1 H NMR (400 MHz, CDCl 3 ): δ 8.04 (d, J=7.6 Hz, 1H), 7.86 (t, J=7.6 Hz 1H), 7.66 (d, J=7.6 Hz, 1H), 4.69 (s, 2H), 4.00 (s, 3H), 3.49 (s, 3H).

›Abbreviations · 12 of 69

Step-2: Synthesis of (6-(methoxy methyl) pyridin-2-yl) methanol (XXIb)

A solution of methyl 6-(methoxymethyl) picolinate XXIa (21 g, 0.12 mol) in THF (200 mL) was cooled to 0° C., added NaBH 4 (13 g, 0.35 mol) portion wise and then reaction mixture was stirred at 25° C. for 3 hours. The progress of the reaction was monitored by TLC. Reaction mixture concentrated at low vacuum and added NaHCO 3 solution (100 mL) and extracted with DCM (3×300 mL). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and evaporated under reduced pressure. Crude compound obtained was purified by gravity column used 230-400 silica mesh and the desired product was eluted at 25-30% ethyl acetate in hexane to afford XXIb as yellow viscous oil. Yield: 16.8 g, 93%. LCMS Calculated. for C 8 H 11 NO 2 is 153.08; Observed 154.25 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.69 (t, J=7.6 Hz, 1H), 7.33 (d, J=7.6 Hz, 1H), 7.14 (d, J=7.6 Hz, 1H), 4.74 (d, J=4.8 Hz, 2H), 4.59 (s, 2H), 3.77 (t, J=4.8, 5.2 Hz, 1H) 3.48 (s, 3H).

Step-3: Synthesis of (6-(methoxy methyl) pyridin-2-yl) methyl methane sulfonate (XXIc)

A solution of (6-(methoxymethyl) pyridin-2-yl) methanol XXIb (7 g, 0.05 mol) in DCM (140 mL) was cooled to 0° C., added TEA (7 g, 0.01 L, 0.07 mol) and MsCl (4 mL, 0.05 mol) dropwise and the reaction mixture was stirred at 0° C. for 2 hours. The progress of the reaction was monitored by TLC. Reaction mixture was quenched with NaHCO 3 solution (100 mL) and extracted with DCM (3×200 mL) and the combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and evaporated under reduced pressure to get brown liquid XXIc which was used as such for the next step without further purification. Yield: 10 g, 92%. 1 H NMR (400 MHz, CDCl 3 ): δ 7.79-7.75 (m, 1H), 7.42 (d, 1H, J=8.0 Hz), 7.38 (d, J=7.6 Hz, 1H), 5.32 (s, 2H), 4.57 (s, 2H), 3.48 (s, 3H), 3.09 (s, 3H).

Step-4: Synthesis of 2-(6-(methoxy methyl) pyridin-2-yl) acetonitrile (XXId)

To a stirred solution of (6-(methoxymethyl) pyridin-2-yl) methyl methane sulfonate XXIc (10 g, 0.043 mol) in DMF (100 mL) was added NaCN (2.3 g, 0.048 mol) portion wise and the reaction mixture was stirred at 24° C. for 3 hours. The progress of the reaction was monitored by TLC. Reaction Mixture quenched with ice cold water (100 mL) and extracted with EtOAc (3×100 mL). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material obtained was purified by flash Chromatography using silica gel 230-400 mesh, the desired product was eluted with 20-25% EtOAc in hexane to afford XXId as yellow viscous oil. Yield: 5.1 g, 73%. LCMS Calculated. for C 9 H 10 N 2 O is 162.08; Observed 163.25 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ) δ 7.7-7.7 (m, 1H), 7.40-7.26 (m, 2H), 4.56 (s, 2H), 3.92 (s, 2H), 3.48 (s, 3H).

Step-5: Synthesis of 2-(6-(methoxy methyl) pyridin-2-yl) ethan-1-amine (XXI)

A solution of 2-(6-(methoxymethyl) pyridin-2-yl) acetonitrile XXId (5.1 g, 0.031 mol) in THF (80 mL) was cooled to 0° C. and added BH 3 ·DMS (15 mL, 0.16 mol) dropwise and the reaction mixture was stirred at 70° C. for 3 hours. The progress of the reaction was monitored by TLC. After completion, the reaction was cooled to room temperature and quenched by MeOH (15 mL). The resulting mixture was stirred at room temperature for 30 min. The mixture was acidified using 1 M HCl solution (15 mL) and concentrated under reduced pressure. The residue obtained was taken in water (20 mL) and EtOAc (50 mL) and DCM (50 mL) were added to remove the impurities. The aqueous layer was separated and basified with 2N NaOH solution. The resulting mixture was extracted with DCM (3×300 mL). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure to afford the desired compound XXI as a brown viscous liquid. The crude compound was used in the next step without further purification. Yield: 3.4 g, 65%. LCMS Calculated. for C 9 H 11 NO 3 is 166.11; Observed 167.30 [M+H] + . 1 H NMR (400 MHz, CDCL3): δ 7.64-7.60 (m, 1H), 7.26 (d, J=8 Hz, 1H), 7.07 (d, J=7.6 Hz, 1H), 4.56 (s, 2H), 3.47 (s, 3H), 3.10-3.07 (m, 2H), 2.93-2.90 (m, 2H).

Synthesis of (1-(6-(2-aminoethyl) pyridin-2-yl) cyclopropyl) methanol (XXII)

Step-1: Synthesis of Ethyl 2-(6-bromopyridin-2-yl) acetate (XXIIa)

To a solution of lithium diisopropylamide (8 g, 0.04 L, 2M, 2.5 eq, 0.07 mol) in THF (150 mL) at −78° C. under nitrogen atmosphere was added 2-bromo-6-methylpyridine (CAS: 5315-25-3, 5 g, 1 eq, 0.03 mol) dropwise and the resulting mixture was stirred at −78° C. for 30 min. This was followed by an addition of diethyl carbonate (9 g, 2.5 eq, 0.07 mol). The resulting mixture was stirred at −40° C. for 6 hours. The progress of the reaction was monitored by TLC for the absence of starting material. The reaction was quenched with saturated solution of NH 4 Cl (60 mL) and the resulting mixture was extracted with ethyl acetate (70 mL×2). The combined organic layer was dried over anhydrous sodium sulfate and concentrated under reduced pressure to afford light brown viscous liquid. The crude compound was purified by column chromatography using silica gel (100-200; 250 g) and EtOAc (0-10%) in hexane as eluent. The peak eluted with 7% of EtOAc in hexane was concentrated to afford the desired product XXIIa as a colorless viscous liquid. Yield: 5.3 g, 70%. LCMS Calculated. for C 9 H 10 BrNO 2 is 242.99; Observed. 244.10 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.53 (t, J=7.6 Hz, 1H), 7.40 (d, J=8.0 Hz, 1H), 7.28 (t, J=7.2 Hz, 1H), 4.19 (q, J=7.2 Hz, 2H), 3.83 (s, 2H), 1.27 (t, J=6.8 Hz, 3H).

Step-2: Synthesis of Ethyl 1-(6-bromopyridin-2-yl) cyclopropane-1-carboxylate (XXIIb)

A stirred solution of ethyl 2-(6-bromopyridin-2-yl) acetate XXIIa (4.2 g, 1 eq, 17 mmol) in DMF (25 mL) was cooled to 0° C. sodium hydride (1.5 g, 60% Wt. 2.2 eq, 38 mmol) was added. To the resulting mixture 1,2-dibromoethane (7.1 g, 2.2 eq, 38 mmol) was added drop wise and stirred at rt for 4 hr. The reaction was monitored by TLC for the absence of starting material. To the reaction mixture (25 mL) ice cold water was added and the resulting mixture was extracted with ethyl acetate (25 mL×2). The combined organic layer was given brine wash, dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure to afford a pale brown viscous liquid. The crude compound was purified by silica gel (100-200; 24 g) column chromatography using EtOAc (0-20%) in hexane. The peak eluted with 7% EtOAc in hexane was concentrated to afford the desired product XXIIb as a pale brown viscous liquid. Yield: 4.1 g, 88%. LCMS Calculated. for C 11 H 12 BrNO 2 is 271.00; Observed. 272.05 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.54 (d, J=7.6 Hz, 1H), 7.50 (t, J=7.6 Hz, 1H), 7.34 (d, J=7.6 Hz, 1H), 4.15 (q, J=7.2 Hz, 2H), 1.67 (t, J=4.4 Hz, 2H), 1.50 (t, J=4.4 Hz, 2H), 1.23 (t, J=7.2 Hz, 3H).

›Abbreviations · 13 of 69

Step-3: Synthesis of Ethyl 1-(6-vinylpyridin-2-yl) cyclopropane-1-carboxylate (XXIIc)

A solution of ethyl 1-(6-bromopyridin-2-yl) cyclopropane-1-carboxylate XXIIb (2.8 g, 1 eq, 10 mmol), potassium trifluoro(vinyl)borate (1.7 g, 1.2 eq, 12 mmol) and potassium phosphate, tribasic (3.3 g, 1.3 mL, 1.5 eq, 16 mmol) in 1,4-dioxane (20 mL) was purged with N 2 gas for 15 min with vigorous stirring. To the reaction was added PdCl 2 (dppf) (0.38 g, 0.05 eq, 0.52 mmol) and heated to 120° C. for 16 hours. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to room temperature and reaction mixture was concentrated under reduced pressure. The residue was added water (70 mL) and the resulting mixture was extracted with ethyl acetate (50 mL×2). The organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford a light brown viscous liquid. The crude compound was purified by combi-flash (230-400, 120 g packed) column chromatography using ethyl acetate in hexane and peak eluted with 5% EtOAc in hexane was concentrated to afford XXIIc as a light brown viscous liquid; Yield: 1 g, 40%. LCMS Calculated. for C 13 H 15 NO 2 is 217.11; Observed. 218.25 [M+H] + 1 H NMR (400 MHz, CDCl 3 ): δ 7.58 (t, J=7.6 Hz, 1H), 7.41 (d, J=7.6 Hz, 1H), 7.19 (d, J=7.6 Hz, 1H), 6.78 (q, J=6.4 Hz, 1H), 6.17 (dd, J=17.4 & 1.6 Hz, 1H), 5.43 (dd, J=11.0 & 1.2 Hz, 1H), 4.15 (q, J=7.2 Hz, 2H), 1.65-1.63 (m, 2H), 1.56-1.53 (m, 2H), 1.21 (t, J=7.2 Hz, 3H).

Step-4: Synthesis of Ethyl 1-(6-formylpyridin-2-yl) cyclopropane-1-carboxylate (XXIId)

To a solution of ethyl 1-(3-vinylphenyl) cyclopropane-1-carboxylate XXIIc (1 g, 1 eq, 5 mmol) in THF (18 mL) and water (40 mL) osmium tetra-oxide (4.68 mL, 2.5% Wt. in isobutanol, 0.1 eq, 0.5 mmol) was added and the reaction was stirred at rt for 30 min. Then sodium metaperiodate (1 g, 0.4 mL, 1.5 eq, 7 mmol) was added and the reaction was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. The mixture was diluted with water (50 mL) and extracted with EtOAc (50 mL×2). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under vacuum to afford XXIId as brown viscous liquid. The crude was used in the next step without further purification. Yield: 0.95 g, 90%). %. LCMS Calculated. for C 13 H 14 O 3 is 219.09; Observed. 220.20 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 10.02 (s, 1H), 7.3 (s, 3H), 4.18 (q, J=7.2 Hz, 2H), 1.73-1.61 (m, 4H), 1.22 (t, J=7.2 Hz, 3H).

Step-5: Synthesis of Ethyl (E)-1-(6-(2-nitrovinyl) pyridin-2-yl) cyclopropane-1-carboxylate (XXIIe)

A stirred solution of ethyl 1-(6-formylpyridin-2-yl) cyclopropane-1-carboxylate XXIId (950 mg, 1 eq, 4.33 mmol) in DCM (20 mL) was cooled to 0° C. To the resulting mixture nitromethane (317 mg, 280 μL, 1.2 eq, 5.20 mmol), triethylamine (2.19 g, 3.02 mL, 5 eq, 21.7 mmol) were added under N 2 gas. The resulting mixture was stirred at rt for 2 h. The reaction mixture was concentrated under reduced pressure to afford a brown viscous liquid. The mixture was dissolved in fresh DCM (20 mL) and was cooled to 0° C. Then triethylamine (2.19 g, 3.02 mL, 5 eq, 21.7 mmol) was added to the reaction and this was followed by a drop-wise addition of mesylchloride (1.49 g, 1.01 mL, 3 eq, 13.0 mmol) under N 2 gas. The reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC analysis which indicated completion of the reaction. The reaction mixture was concentrated under reduced pressure and water (15 mL) was added and extracted with ethyl acetate (15 mL×2). The combined organic layer was given brine wash, dried over anhydrous sodium sulphate, and concentrated under reduced pressure to afford XXIIe as brown viscous liquid. The crude product was taken for next step without further purification. Yield: 1.0 g, 86%. LCMS Calculated. for C 13 H 14 N 2 O 4 is 262.10; Observed. 263.20 [M + +H]

Step-6: Synthesis of (1-(6-(2-Aminoethyl) pyridin-2-yl) cyclopropyl) methanol (XXII)

To an ice-cold solution of LiAlH 4 (20 mL, 1M, 4 eq,) in dry diethyl ether (80 mL) solution was dropwise added a solution of ethyl (E)-1-(6-(2-nitrovinyl) pyridin-2-yl) cyclopropane-1-carboxylate XXIIe (1 g, 1 eq, 4 mmol) in dry diethyl ether (7 mL). The resulting mixture was stirred at rt for 1 hr. The reaction was monitored by TLC analysis which indicated the completion of reaction. The reaction mixture was cooled to 0° C. and quenched water (2 mL) followed by with 15% KOH solution (3 mL). To the mixture was added ethyl acetate (100 mL) and stirred at rt for 20 min. The reaction mixture was filtered, and the residue was washed with 100 mL of ethyl acetate. The combined filtrate was concentrated under reduced pressure to afford a desired compound as pale brown viscous liquid XXII. The crude was used in the next step without further purification. Yield: 0.6 g, 80%. LCMS Calculated. for C 11 H 16 N 2 O is 192.13; Observed. 193.25 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 7.52-7.50 (m, 1H), 6.98 (d, J=8.0 Hz, 1H), 6.70 (d, J=8.0 Hz, 1H), 3.83 (d, J=3.6 Hz, 4H), 3.13 (t, J=6.8 Hz, 2H), 2.90 (t, J=6.4 Hz, 2H); 1.05-1.01 (m, 2H) 0.90-0.85 (m, 2H).

Synthesis of 2-(1-(2-methoxyethyl)-1H-pyrazol-3-yl) ethan-1-amine (XXIII)

Step-1: Synthesis of 1-(2-methoxyethyl)-1H-pyrazole-3-carbaldehyde) XXIIIa

1-bromo-2-methoxyethane (5.2 g, 0.037 mol) was added dropwise to a stirred solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1: 3.0 g, 0.031 mol) and CS 2 CO 3 (20.0 g, 0.0624 mol) in DMF (50 mL) at 0° C. Then reaction mixture was stirred at rt for 3 h. The progress of the reaction was monitored by TLC. Then the reaction mixture was diluted with water (500 mL) and extracted with ethyl acetate (3×50 mL). The combined organic layer was washed with water (50 mL). the combined organic layer was dried over anhydrous Na 2 SO 4 and evaporated under reduced pressure to get crude product. Crude product obtained was purified by biotage column chromatography using silica gel (230-400 mesh) and the desired product was eluted at 5-20% ethyl acetate in hexane to afford XXIIIa as colourless liquid Yield: 3.2 g, 66%. LCMS Calculated. for C 7 H 10 N 2 O is 154.17; Observed. 155.2 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 9.97 (S, 1H), 7.54 (d, J=1.6 Hz, 1H), 6.80 (d, J=2.0 Hz, 1H), 4.38 (t, J=4.8 Hz, 2H), 3.78 (t, J=5.6 Hz, 2H), 3.34 (S, 3H).

›Abbreviations · 14 of 69

Step-2: Synthesis of (E)-1-(2-methoxyethyl)-3-(2-nitrovinyl)-1H-pyrazole XXIIIb

A mixture of 1-(2-methoxyethyl)-1H-pyrazole-3-carbaldehyde XXIIIa (3.2 g, 0.019 mol), nitromethane (35 g, 31 mL, 0.57 mol) and ammonium acetate (2.2 g, 0.029 mol) were taken in a round bottom flask and the reaction mixture was stirred at 100° C. for 2 h. Then the progress of the reaction was monitored by TLC for the absence of staring material. The reaction mixture was evaporated under vacuum to get crude residue which was purified by biotage column chromatography using silica (230-400 mesh). The desired product was eluted at 5-20% ethyl acetate in hexane as eluent to afford desired product as colourless liquid XXIIIb. Yield: 2.4 g, 60%. LCMS Calculated. for C 8 H 11 N 3 O 3 is 197.19; Observed. 198.2 [M+H] + .

Step-3: Synthesis of 2-(1-(2-methoxyethyl)-1H-pyrazol-3-yl) ethan-1-amine XXIII

To a stirred solution of LAH 1.0M in THF (50 mL, 0.048 mol) in diethyl ether (125 mL), was added (E)-1-(2-methoxyethyl)-3-(2-nitrovinyl)-1H-pyrazole XXIIIb (2.4 g, 0.012 mol) in THF (5 ml) at 0° C., under argon atmosphere. The reaction mixture was stirred at rt for 2 h. Then the progress of the reaction was monitored by TLC. The reaction mixture was quenched with water (2.4 ml) at 0° C. followed by addition of 15% KOH solution (2.4 mL) and water (8 mL). Then the reaction mixture was filtered and washed the solid with ethyl acetate (3×100 mL). The combined organic layer was dried over anhydrous Na 2 SO 4 and evaporated under reduced pressure to get crude XXIII as pale-yellow liquid. Yield: 1.5 g, 83% crude.

Synthesis of (1-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopropyl) methanol. (XXIV)

Step-1: Synthesis of methyl 1-(3-formyl-1H-pyrazol-1-yl) cyclopropane-1-carboxylate (XXIVa)

Methyl 2,4-dibromobutanoate (CAS: 29547-04-4, 35 g, 0.14 mol) was added to the stirred solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1, 10 g, 0.1 mol), K 2 CO 3 (58 g, 0.42 mol) in DMF (100 mL) at 0° C. Then the reaction mixture was stirred at room temperature for 16 hours. Progress of the reaction was monitored by TLC analysis. Reaction mixture was quenched with cold water and extracted with ethyl acetate (2×200 mL). The combined organic layer was washed with water (3×100 mL) and followed by brine solution, dried over anhydrous sodium sulphate, and concentrated. The crude material was purified by combi-flash using 80 g column, eluted with 0-25% ethyl acetate:hexane, to afford XXIVa as white solid. Yield: 13.5 g, 67%. LCMS Calculated. for C 9 H 10 N 2 O 3 is 194.07; Observed. 195.15 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 9.97 (s, 1H), 7.58 (s, 1H), 6.82 (s, 1H), 3.70 (d, J=6.8 Hz, 3H), 1.93-1.90 (m, 2H), 1.73-1.70 (m, 2H).

Step-2: Synthesis of methyl (E)-1(3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopropane-1-carboxylate (XXIVb)

To a stirred solution of ethyl 1-(3-formyl-1H-pyrazol-1-yl) cyclopropane-1-carboxylate XXIVa (1 g, 0.0051 mol) in toluene (25 mL) was added ammonium acetate (0.6 g, 0.0077 mol) and nitromethane (3.1 g, 0.051 mol), and the resulting mixture was stirred at 100° C. for 16 hrs. The progress of the reaction was monitored by TLC. After completion of the reaction, reaction mixture was quenched with 1N HCl solution (20 mL), extracted with ethyl acetate (2×20 mL). The combined organic layer was washed with brine solution (15 mL), dried over anhydrous sodium sulphate. Solvent was removed under vacuum. The crude compound was passed through flash column and eluted with 10-20% ethyl acetate in hexane to afford XXIVb as white solid. Yield: 1.2 g, 35%. LCMS Calculated. for C 10 H 11 N 3 O 4 is 237.07; Observed. 238.15 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.95 (d, J=13.6 Hz, 1H), 7.61 (d, J=13.6 Hz, 1H), 7.57 (d, J=2.4 Hz, 1H), 6.56 (d, J=2.4 Hz, 1H), 3.7 (s, 3H), 1.91-1.87 (m, 2H), 1.7-1.66 (m, 2H).

Step-3: Synthesis of (1-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopropyl) methanol (XXIV)

Methyl (E)-1 (3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopropane-1-carboxylate XXIVb (0.430 g, 0.0018 mol) in THF (5 mL) was added dropwise to a stirred solution of LAH (1M in THF, 7.25 ml, 0.0072 mol) in Et 2 O (10 mL) at 0° C. and stirred the reaction mixture for 1 hr at room temperature. The progress of the reaction was monitored by TLC. After completion of the reaction, the reaction mixture was cooled to 0° C. and quenched with water (0.4 mL) followed by 15% KOH solution (0.4 mL), water (1.2 mL) and the reaction mixture was stirred for 15-20 min. Reaction mixture was filtered and washed with EtOAc (3×20). The combined organic layer was dried over anhydrous sodium sulphate. Concentrated the solvent under reduced pressure to get pale yellow oil XXIV. Yield: 0.329 g, 93.8%. LCMS Calculated. for C 9 H 15 N 3 O is 181.24; Observed. 182.25 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ 7.40 (d, J=2.4 Hz, 1H), 6.05 (d, J=2.0 Hz, 1H), 3.74 (s, 3H), 2.98 (t, J=6.8 Hz, 2H), 2.76 (t, J=7.2 Hz, 2H), 1.26-1.23 (m, 2H), 1.09-1.08 (m, 2H).

Synthesis of (2-(6-(2-aminoethyl) pyridin-2-yl) cyclopropyl) methanol (XXV)

Step-1: Synthesis of ethyl (E)-3-(6-bromopyridin-2-yl) acrylate (XXVa)

To a suspension of sodium hydride (3.2 g, 55% wt, 1.35 eq, 73 mmol) in THF (300 mL) was dropwise added ethyl 2-(diethoxy phosphoryl) acetate (CAS:867-13-0, 15 g, 1.25 eq, 67 mmol) at 0° C. After 30 min, solution of 6-bromopicolinaldehyde (10 g, 1 eq, 54 mmol) in 30 mL THF was added and the reaction mixture was stirred at rt for 4 h. the progress of reaction was monitored by TLC analysis. After completion of the reaction, the reaction was cooled to 0° C. and water (75 mL) was dropwise added. The resulting mixture was extracted with ethyl acetate (75 mL×2). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the light brown viscous liquid. The crude compound was purified by silica gel (100-200; 150 g) column chromatography using EtOAc (0-20%) in hexane. The peak eluted with 10% of EtOAc in hexane was concentrated to afford XXVa as an off white solid. Yield: 10 g, 73%. LCMS Calculated. for C 10 H 10 BrNO 2 is 254.99; Observed. 256.05 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.60-7.54 (m, 2H), 7.45 (d, J=8.0 Hz, 1H), 7.35 (d, J=7.6 Hz, 1H), 6.96 (d, J=15.2 Hz, 1H), 4.27 (q, J=7.2 Hz, 2H), 1.33 (t, J=7.2 Hz, 3H.

›Abbreviations · 15 of 69

Step-2: Synthesis of ethyl 2-(6-bromopyridin-2-yl) cyclopropane-1-carboxylate (XXVb)

To a solution of Trimethylsulfoxonium Iodide (CAS: 1774-47-6, 19 g, 2.2 eq, 86 mmol) in DMSO (140 mL) was added sodium hydride portion wise (2.2 g, 55% Wt. 1.3 eq, 51 mmol) and the resulting mixture was stirred for one hour at room temperature. A solution of ethyl (E)-3-(6-bromopyridin-2-yl) acrylate (10 g, 1 eq, 39 mmol) in mixture of DMSO (60 mL) and THF (60 mL) was slowly added to the reaction. The resulting mixture was stirred for four hours at room temperature. The progress of the reaction was monitored by TLC analysis. After completion of the reaction, 1N HCl (10 mL) was added, and the reaction mixture extracted with diethyl ether (30 mL×2). The combined organic layer was dried over anhydrous sodium sulphate, the solvent was removed under vacuo. The crude compound was passed through a plug of silica gel (100-200; 120 g) in ethyl acetate in hexane (7%) to afford the desired compound XXVb as a colourless viscous liquid. Yield: 6.0 g, 60%. 1 H NMR (400 MHz, CDCl 3 ): δ 7.57-7.39 (m, 1H), 7.27-7.26 (m, 1H), 7.18 (d, J=7.6 Hz, 1H), 4.25-4.12 (m, 2H), 2.58-2.51 (m, 2H), 2.29-2.25 (m, 2H), 1.34-1.23 (m, 3H)

Step-3: Synthesis of Ethyl 2-(6-vinylpyridin-2-yl) cyclopropane-1-carboxylate (XXVc)

A solution of ethyl 2-(6-bromopyridin-2-yl) cyclopropane-1-carboxylate (3.9 g, 1 eq, 14 mmol), Potassium Vinyl trifluoroborate (CAS: 13082-77-4, 2.1 g, 1.1 eq, 16 mmol) and potassium phosphate, tribasic (4.6 g, 1.8 mL, 1.5 eq, 22 mmol) in 1,4 dioxane (60 mL) was purged with N 2 gas for 15 min with vigorous stirring. To the reaction was added PdCl 2 (dppf) (0.53 g, 0.05 eq, 0.72 mmol) and the seal tube was closed and heated to 120° C. for 16 hours. The progress of the reaction was monitored by TLC analysis. After completion of the reaction, it was cooled to room temperature and reaction mixture was concentrated under reduced pressure. To the residue obtained was added water (80 mL) and the resulting mixture was extracted with ethyl acetate (50 mL×2). The organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford a light brown viscous liquid. The crude compound was purified by combi-flash (230-400, 120 g packed) column chromatography using ethyl acetate in hexane and peak eluted with 5% EtOAc in hexane was concentrated to afford XXVc as a pale-yellow viscous liquid. Yield: 1.8 g, 57%. LCMS Calculated. for C 13 H 15 NO 2 is 217.11; Observed. 218.20 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.51 (t, J=7.2 Hz, 1H), 7.10-7.07 (m, 2H), 6.78-6.68 (m, 1H), 6.78-6.68 (m, 1H), 5.42-5.39 (m, 1H), 4.19-4.14 (m, 1H), 2.63-2.57 (m, 2H), 2.31-2.29 (m, 1H), 1.66-1.56 (m, 2H), 1.34-1.26 (m, 3H).

Step-4: Synthesis of ethyl 2-(6-formylpyridin-2-yl) cyclopropane-1-carboxylate (XXVd)

To a solution of ethyl 2-(6-vinylpyridin-2-yl) cyclopropane-1-carboxylate XXVc (1.6 g, 1 eq, 7.4 mmol) in THF (4.4 mL) and water (10 mL) a solution of osmium tetroxide (7.4 mL g, 2.5% wt % in isobutanol, 0.1 eq, 0.74 mmol) was added and the reaction was stirred at rt for 30 min. Then, sodium metaperiodate (2.4 g, 0.59 mL, 1.5 eq, 11 mmol) was added and the reaction was stirred at rt for 2 h. Then the progress of the reaction was monitored by TLC analysis which indicated completion of the reaction. The mixture was diluted with water (35 mL) and extracted with EtOAc (30 mL×2). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under vacuum to afford XXVd as brown viscous liquid. The crude product obtained was used as such in the next step without further purification. Yield: (1.56 g, 97%). LCMS Calculated. for C 12 H 13 NO 3 is 219.09; Observed. 220.20 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 9.95 (s, 1H), 7.76-7.72 (m, 2H), 7.47-7.45 (m, 1H), 4.27-4.16 (m, 2H), 2.70-2.65 (m, 2H), 2.39-2.37 (m, 2H), 1.30-1.27 (m, 3H).

Step-5: Synthesis of ethyl (E)-2-(6-(2-nitrovinyl) pyridin-2-yl) cyclopropane-1-carboxylate (XXVe)

A stirred solution of ethyl 2-(6-formylpyridin-2-yl) cyclopropane-1-carboxylate XXVd (1.72 g, 1 eq, 7.85 mmol) in DCM (15 mL) was cooled to 0° C. To the resulting mixture nitromethane (575 mg, 1.2 eq, 9.41 mmol) and triethylamine (2.19 mL, 2.0 eq, 15.7 mmol) were added under N 2 gas. The resulting mixture was stirred at rt for 2 h. The reaction mixture was concentrated under reduced pressure to afford a brown viscous liquid. The mixture was dissolved in fresh DCM (10 mL) and was cooled to 0° C. Then triethylamine (3.02 mL, 5 eq, 21.7 mmol) was added to the reaction and this was followed by a drop-wise addition of mesyl chloride (1.49 g, 1.01 mL, 3 eq, 13.0 mmol) under N 2 gas. The reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC analysis which indicated completion of reaction. The reaction mixture was concentrated under reduced pressure and water (15 mL) was added and extracted with ethyl acetate (15 mL×2). The combined organic layer was given brine wash, dried over anhydrous sodium sulphate, and concentrated under reduced pressure to afford XXVe as a brown viscous liquid. The crude was used in the next step without further purification. Yield: 1.87 g, 100%.

Step-6: Synthesis of (2-(6-(2-Aminoethyl) pyridin-2-yl) cyclopropyl) methanol (XXV)

To a cooled LiAlH 4 (30 mL, 1M, 4 eq,) in dry diethyl ether (160 mL) using ice bath, was added dropwise a solution of ethyl (E)-2-(6-(2-nitrovinyl) pyridin-2-yl) cyclopropane-1-carboxylate (2 g, 1 eq, 8 mmol) in dry diethyl ether (7 mL). The resulting mixture was stirred at rt for 2 h. The reaction was monitored by TLC analysis which indicated the completion of reaction. The reaction mixture was cooled to 0° C. and quenched water (3 mL) followed by with 15% KOH solution (15 mL). To the mixture was added ethyl acetate (75 mL) and stirred at rt for 20 min. The reaction mixture was filtered, and the residue was washed with 100 mL of ethyl acetate. The combined filtrate was concentrated under reduced pressure to afford XXV as pale brown viscous liquid. The crude was used as such in the next step without further purification. Yield: 1.03 g, 100%. LCMS Calculated. for C 11 H 16 N 2 O is 192.13; Observed. 193.25 [M+H] + .

›Abbreviations · 16 of 69

Synthesis of 2-(6-ethylpyridin-2-yl) ethan-1-amine (XXVI)

Step-1: Ethyl 2-(6-bromopyridin-2-yl) acetate (XXVIa)

To a stirred solution of lithium diisopropylamide (110 mL, 2 molar, 220 mmol) in dry THF (500 mL) at −78° C. under inert atmosphere was dropwise added 2-bromo-6-methylpyridine (CAS:5315-25-3, 15 g, 87 mmol) and the resulting mixture was stirred at −78° C. for 30 min. This was followed by an addition of diethyl carbonate (CAS: 105-58-8, 26 g, 220 mmol) mixture was stirred at −40° C. for 4 hours. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with saturated solution of NH 4 Cl (150 mL), and the resulting mixture was extracted with ethyl acetate (170 mL×2). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford light brown viscous liquid. The crude compound was purified by silica gel (100-200) column chromatography using ethyl acetate (0-10%) in n-hexane. The peak eluted with 7% of ethyl acetate in n-hexane was concentrated to afford the desired product XXVIa as a colorless viscous liquid. Yield: 12.2 g (57%); LCMS Calculated. for C 9 H 11 BrNO 2 is 246.00, Observed, 246.15; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.53 (t, J=7.6 Hz, 1H), 7.40 (d, J=8.0 Hz, 1H), 7.28 (t, J=7.6 Hz, 1H), 4.19 (q, J=7.2 Hz, 2H), 3.83 (s, 2H), 1.27 (t, J=7.2 Hz, 3H).

Step-2: Ethyl 2-(6-vinylpyridin-2-yl) acetate (XXVIb)

A solution of ethyl 2-(6-bromopyridin-2-yl) acetate XXVIa (3 g, 10.0 mmol), potassium trifluoro(vinyl)borate (2 g, 11.0 mol) and potassium phosphate, tribasic (4 g, 20 mmol) in 1,4 dioxane (40 mL) was purged with N 2 gas for 15 min with vigorous stirring. To the reaction was added PdCl 2 (dppf) (0.4 g, 0.6 mmol) and the seal tube was closed and heated to 120° C. for 16 hours. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to room temperature and reaction mixture was concentrated under reduced pressure. The residue was added water (70 mL) and the resulting mixture was extracted with ethyl acetate (75 mL×2). The organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford a light brown viscous liquid. The crude compound was purified by combi-flash (230-400) column chromatography using ethyl acetate in n-hexane and peak eluted with 3% ethyl acetate in hexane was concentrated to afford the desired compound XXVIb as a colorless viscous liquid. Yield: 2.0 g (90%); LCMS Calculated. for C 11 H 14 NO 2 is 192.10, Observed, 192.25; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.62 (t, J=7.6 Hz, 1H), 7.25 (d, J=7.6 Hz, 1H), 7.17 (d, J=7.6 Hz, 1H), 6.80 (q, J=6.4 Hz, 1H), 6.19 (d, J=17.6 Hz, 1H), 5.47 (d, J=10.8 Hz, 1H), 4.19 (q, J=7.2 Hz, 2H), 1.27 (t, J=7.2 Hz, 3H).

Step-3: 2-(6-Vinylpyridin-2-yl) ethan-1-ol. (XXVIc)

To a stirred solution of ethyl 2-(6-vinylpyridin-2-yl) acetate XXVIb (2 g, 10.0 mmol) in methanol (30 mL) under nitrogen atmosphere was portion wise added at 0° C. sodium borohydride (2 g, 50 mmol). The resulting mixture was stirred at 0° C. for 30 min and at room temperature for 6 h. The progress of the reaction was monitored by TLC analysis which indicates completion of reaction. To the reaction mixture saturated NaHCO 3 solution (50 mL) was added and extracted with ethyl acetate (75 mL×2). The organic layer was given brine (35 mL) wash, dried over anhydrous sodium sulphate, and concentrated under reduced pressure to afford XXVIc as colorless viscous liquid. The crude was taken for next step without further purification. Yield: 1.2 g (80%); LCMS Calculated. for C 9 H 12 NO is 150.09, Observed, 150.00; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.59 (t, J=7.6 Hz, 1H), 7.19 (d, J=7.6 Hz, 1H), 7.02 (d, J=8.0 Hz, 1H), 6.81-6.74 (m, 1H), 6.16 (d, J=17.6 Hz, 1H), 5.47 (d, J=10.8 Hz, 1H), 4.03 (t, J=5.6 Hz, 2H), 3.01 (t, J=5.6 Hz, 2H).

Step-4: 2-(6-Vinylpyridin-2-yl) ethyl methane sulfonate (XXVId)

To a stirred solution of 2-(6-vinylpyridin-2-yl) ethan-1-ol XXVIc (1.2 g, 8.0 mmol) in DCM (30 mL) triethylamine (4.5 mL, 32 mmol) was added under nitrogen atmosphere. The resulting mixture was cooled to 0° C. and mesyl-Cl (0.81 mL, 10.0 mmol) was drop wise added. The reaction mixture was stirred at 0° C. for 30 min. The progress of the reaction was monitored by TLC analysis which indicates completion of reaction. To the reaction mixture water (30 mL) was added and extracted with DCM (30 mL×3). The organic layer was washed with brine (25 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure to afford XXVId as brown viscous liquid. The crude was taken for next step without further purification. Yield: 1.7 g (93%); LCMS Calculated. for C 10 H 14 NO 3 S is 228.08, Observed, 228.20; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.60 (t, J=7.6 Hz, 1H), 7.22 (d, J=8.0 Hz, 1H), 7.08 (d, J=8.0 Hz, 1H), 6.82-6.75 (m, 1H), 6.22 (d, J=17.6 Hz, 1H), 5.48 (d, J=10.8 Hz, 1H), 4.73-4.66 (m, 2H), 3.21 (t, J=6.4 Hz, 2H), 2.88 (s, 3H).

Step-5: 2-(2-Azidoethyl)-6-vinylpyridine (XXVIe)

To a stirred solution of 2-(6-vinylpyridin-2-yl) ethyl methane sulfonate XXVId (1.7 g, 7.5 mmol) in DMF (20 mL) sodium azide (1.5 g, 22 mmol) was added under nitrogen atmosphere. The resulting mixture was stirred at 100° C. for 4 h. The progress of the reaction was monitored by TLC analysis. After completion of the reaction water (30 mL) was added and extracted with ethyl acetate (30 mL×3). The organic layer was washed with brine (25 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure to afford brown viscous liquid. The crude product was purified by combi-flash (silica gel 230-400) column chromatography using ethyl acetate (0-40%) in n-hexane. The peak eluted with 5% ethyl acetate was concentrated to afford the desired compound XXVIe as a colorless viscous liquid. Yield: 0.87 g (67%); LCMS Calculated. for C 9 H 11 N 4 is 175.10, Observed, 175.25; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.58 (t, J=7.6 Hz, 1H), 7.21 (d, J=7.6 Hz, 1H), 7.06 (d, J=7.6 Hz, 1H), 6.84-6.77 (m, 1H), 6.22 (d, J=17.6 Hz, 1H), 5.47 (d, J=10.8 Hz, 1H), 3.74 (t, J=6.8 Hz, 2H), 3.06 (t, J=6.8 Hz, 2H).

›Abbreviations · 17 of 69

Step-6: 2-(6-Ethylpyridin-2-yl) ethan-1-amine (XXVI)

In RB flask 2-(2-azidoethyl)-6-vinylpyridine XXVIe (0.87 g, 5.0 mmol) was dissolved in methanol (30 mL) and purged with nitrogen for 10 minutes. To this solution palladium on carbon (450 mg, 10%) was added. The reaction was evacuated and refilled with H 2 gas, this procedure was repeated for 3-4 times and the reaction was stirred at room temperature for 16 h maintaining H 2 atmosphere. The progress of reaction was monitored by TLC. After completion, the reaction mixture was filtered through a celite bed and washed with fresh methanol. The combined filtrate was concentrated under vacuo to afford the desired compound XXVI as a pale-yellow viscous liquid which was taken for the next step without further purification. Yield: 0.67 g (89%); LCMS Calculated for C 9 H 15 N 2 is 151.13, Observed, 151.25; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.54-7.46 (m, 1H), 7.01-6.94 (m, 2H), 3.09-2.73 (m, 6H), 1.28 (m, 3H).

Synthesis of 2-(3-fluoro-6-methylpyridin-2-yl) ethan-1-amine (XXVII)

Step-1: 3-Fluoro-6-methylpicolinaldehyde (XXVIIa)

A two neck RB flask equipped with two way stop-cock, septum and magnetic bar was charged with 2-bromo-3-fluoro-6-methylpyridine (CAS: 374633-36-0; 1.66 g, 8.74 mmol) and dry toluene (10 mL) under N 2 at RT. The resulting mixture was cooled to −78° C. and n-butyllithium (3.5 mL, 2M in THF, 8.74 mmol) was added dropwise and stirred the reaction mixture maintaining temperature −78° C. under proper N 2 atmosphere. This was followed by slow addition of dry DMF (1.92 g, 26.2 mmol) at −78° C. under N 2 atmosphere. The reaction was stirred at −78° C. under N 2 atmosphere for additional one hour and at −50° C. for 2 h. The progress of the reaction was monitored by TLC analysis. The reaction was quenched by slow addition sat. ammonium chloride solution at −78° C. The resulting mixture was extracted with ethyl acetate (10 mL×3). The combined organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure to get the crude brown semi-solid. The crude was subjected to silica gel (230-400 mesh) column chromatography purification using 0-10% ethyl acetate in n-hexane to afford the desired compound XXVIIa as off-white solid. Yield: 0.175 g (14.4%); LCMS Calculated. for C 7 H 7 FNO is 140.05, Observed, 140.25; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 10.19 (s, 1H), 7.49-7.38 (m, 2H), 2.64 (s, 3H).

Step-2: (E)-3-fluoro-6-methyl-2-(2-nitrovinyl) pyridine (XXVIIb)

To a solution of 3-fluoro-6-methylpicolinaldehyde XXVIIa (0.4 g, 3 mmol) in DCM (4 mL) under N 2 at room temperature were added triethylamine (0.6 g, 6 mmol) and nitromethane (0.2 mL, 3 mmol) under N 2 . The reaction was stirred at RT for 30 min and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated to dryness under vacuo and the residue was dissolved in fresh dry DCM (4 mL). The mixture was cooled to 0° C. under N 2 atmosphere and TEA (1 mL, 9 mmol) was added. This was followed by slow addition of mesyl chloride (0.7 mL, 9 mmol) at 0° C. under N 2 . The reaction was stirred at room temperature for 1 h under N 2 at RT. The progress of the reaction was monitored by TLC analysis. After completion, water (10 mL) was added, and the resulting mixture was extracted with DCM (5 mL×3). The combined organic layer was given brine wash, dried over anhydrous sodium sulphate, and concentrated under reduced pressure to get the brown semisolid. The crude was subjected to silica gel column (230-400) chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound XXVIIb as a colorless viscous liquid. Yield: 93 mg (20%); 1 H NMR (400 MHz, CDCl 3 ): δ 8.15 (d, J=13.2 Hz, 1H), 8.04 (d, J=13.6 Hz, 1H), 7.39 (t, J=9.2 Hz, 1H), 7.25 (t, J=3.6 Hz, 1H), 2.57 (s, 3H).

Step-3: 2-(3-fluoro-6-methylpyridin-2-yl) ethan-1-amine (XXVII)

To a two neck 100 mL round bottom flask was charged with dry diethyl ether under N 2 and the solvent was cooled to 0° C. To this was added LAH (1 mL, 2M in THF, 2.0 mmol) followed by an addition of solution of 1(E)-3-fluoro-6-methyl-2-(2-nitrovinyl) pyridine XXVIIb (93 mg, 0.51 mmol) in dry diethyl ether:THF (1:1, 2 mL). The resulting mixture was stirred 1 h at RT under N 2 . The progress of the reaction was monitored using TLC analysis. After completion, the reaction was cooled to 0° C. and quenched with water (0.2 mL) and ethyl acetate (5 mL). The solution was filtered through celite bed. Bed was given wash with ethyl acetate followed by 10% MeOH in DCM. The combined filtrate was dried over anhydrous sodium sulphate and concentrated under vacuo to get XXVII as brown semisolid crude material. The crude product obtained was taken for the next step without further purification. Yield: 47 mg (60%).

Synthesis of 7-amino-2-ethyl-3-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXVIII)

Step-1: 2-methyl-3-oxopentanenitrile (XXVIIIa)

To a stirred solution of lithium diisopropylamide (110 mL, 2 M, 60 mmol) in dry THF (56 mL) at −78° C. under inert atmosphere was dropwise added propionitrile (CAS: 107-12-0; 4 mL, 60 mmol) and the resulting mixture was stirred at −78° C. for 1 h. This was followed by an addition of ethyl propionate (CAS: 105-37-3; 6 mL, 50 mmol) and the reaction was stirred at −78° C. for additional 1 hour. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with saturated solution of NH 4 Cl (20 mL), and the resulting mixture was extracted with ethyl acetate (50 mL×2). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford as light brown viscous liquid. The crude compound was purified by silica gel (100-200) column chromatography using ethyl acetate (0-10%) in n-hexane. The peak eluted with 7% of ethyl acetate in n-hexane was concentrated to afford the desired product XXVIIIa as a colorless viscous liquid. The crude was taken for next step without further purification. Yield: 4.3 g (71%).

›Abbreviations · 18 of 69

Step-2: 5-ethyl-4-methyl-1H-pyrazol-3-amine (XXVIIIb)

To a stirred solution of 2-methyl-3-oxopentanenitrile XXVIIIa (4.3 g, 39 mmol) in ethanol (4 mL) was added hydrazine hydrate (1:1) (2.9 g, 58 mmol) at room temperature and the reaction mixture was stirred at 120° C. for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was cooled to room temperature and concentrated to remove solvent. Obtained residue was dissolved in water (50 mL) and extracted with 10% methanol in DCM (100 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated to afford the desired product XXVIIIb as a yellowish viscous liquid. The crude was taken for next step without further purification. Yield: 5.4 g (69%).

Step-3: 7-amino-2-ethyl-3-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXVIIIc)

To a stirred solution of 5-ethyl-4-methyl-1H-pyrazol-3-amine XXVIIIb (5.4 g, 32 mmol) in pyridine (30 mL) was added 2-(bis (methylthio) methylene) malononitrile Ia (4.0 g 32 mmol). Then reaction mixture was stirred at 120° C. for 3 hours. The progress of the reaction was monitored by TLC. After completion, reaction mixture was cooled to room temperature then poured into ice cold water (100 mL). The formed solid was filtered and washed with ice cold water. solid was dried under vacuum to afford the desired product XXVIIIc as an off-white solid. The crude was taken for next step without further purification. Yield: 5.3 g (68%); LCMS Calculated. for C 11 H 14 N 5 S is 248.32, Observe, 248.20; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 6.19 (bs, 2H), 2.75 (q, J=7.6 Hz, 2H), 2.62 (s, 3H), 2.19 (s, 3H), 1.3 (t, J=7.6 Hz, 3H).

Step-4: 7-amino-2-ethyl-3-methyl-5-(methylsulfonyl)-1,2-dihydropyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXVIII)

To a stirred solution of 7-amino-2-ethyl-3-methyl-5-(methylthio)-1,2-dihydropyrazolo[1,5-a] pyrimidine-6-carbonitrile XXVIIIc (5.4 g, 22 mmol) in DCM (60 mL) was portion wise added mCPBA (15 g, 87 mmol) at 0° C. under inert atmosphere. The resulting mixture was stirred at room temperature for 4 hours. The progress of reaction was monitored by TLC analysis. After completion, the reaction mixture was quenched with NaHCO 3 (50 mL) and extracted with DCM (50 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure to afford the desired product XXVIII as an off-white solid. The obtained solid was washed with n-hexane (10 mL×2). The crude solid was taken for next step without further purification. Yield: 1.0 g (18%); LCMS Calculated. for C 11 H 16 N 5 O 2 S is 282.33, [M+H] + , Observed, 280.25; [M+H] −2

Synthesis of 7-amino-2-cyclopropyl-3-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile. (XXIX)

Step-1: 3-cyclopropyl-2-methyl-3-oxopropanenitrile (XXIXa)

To a stirred solution of LDA (30 mL, 2 M, 50 mmol) in dry THF (70 mL) at −78° C. under inert atmosphere was added a solution of propenonitrile (CAS:107-12-0; 3 g, 50 mmol) in THF (20 mL) and the resulting mixture was stirred at −78° C. for 1 h. This was followed by an addition of ethyl cyclopropane carboxylate (6 g, 50 mmol) and the mixture was stirred at −70° C. for 1 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with saturated solution of NH 4 Cl (50 mL) and the resulting mixture was extracted with ethyl acetate (50 mL×3) The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford XXIXa as light brown viscous liquid. The crude compound was used as such in the next step without further purification. Yield: 4.5 g (70%).

Step-2: 5-cyclopropyl-4-methyl-1H-pyrazol-3-amine (XXIXb)

To a stirred solution of 3-cyclopropyl-2-methyl-3-oxopropanenitrile XXIXa (4.2 g, 34 mmol) in ethanol (50 mL) was added hydrazine hydrate (3.3 mL, 50% Wt., 34 mmol) and the reaction mixture was heated at 90° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was cooled to rt and evaporated under reduced pressure. The residue obtained was diluted with water (50 mL) and extracted with ethyl acetate (50 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material XXIXb obtained was used as such in the next step without further purification. Yield: 4.2 g (90%); LCMS Calculated. for C 7 H 12 N 3 is 138.20, Observed, 138.25; [M+H] +

Step-3: 7-amino-2-cyclopropyl-3-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitile (XXIXc)

To a stirred solution of 2-(bis(methylthio)methylene) malononitrile Ia (5.2 g, 31 mmol) in pyridine (35 mL) was added 5-cyclopropyl-4-methyl-1H-pyrazol-3-amine XXIXb (4.2 g, 31 mmol) and the reaction mixture was heated at 120° C. for 1 h under N 2 atmosphere. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and poured into ice cold water (50 mL). The precipitate formed was filtered, dried, and washed with cold mixture of ethyl acetate (10%) and n-hexane (90%), dried under vacuo to afford the desired compound as a pale-yellow solid. The crude compound XXIXc obtained was taken for the next step without further purification. Yield: 4.1 g (52%); LC_MS Calculated. for C 12 H 14 N 5 S is 260.34, Observed, 260.20; [M+H] +

Step-4: 7-amino-2-cyclopropyl-3-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXIX)

To a solution of 7-amino-2-cyclopropyl-3-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXIXc (1 g, 4 mmol) in DCM (20 mL) at 0° C. under N 2 atmosphere was added mCPBA (4 g, 60% Wt., 20 mmol) and the resulting mixture was stirred at rt for 4 h. The progress of the reaction was monitored by TLC analysis. After completion of the reaction, added sat. NaHCO 3 solution (50 ml) and the mixture was stirred vigorously for 15 min. The organic layer was separated and given fresh wash of sat. NaHCO 3 solution (50 mL×3). The organic layers were separated, washed with brine, dried over anhydrous Na 2 SO 4 and concentrated under vacuo to afford the desired compound as a pale-yellow solid. The compound XXIX was used in the next step without further purification. Yield: 0.35 g (30%), LC_MS Calculated. for is 292.34, Observed, 292.20; [M+H] +

›Abbreviations · 19 of 69

Synthesis of 7-amino-2-(difluoro methyl)-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXX)

Step-1: 2-ethyl-4,4-difluoro-3-oxobutanenitrile (XXXa)

To a solution of LiHMDS (2.4 g, 14 mmol) in THF (200 mL) at −78° C. under inert atmosphere was drop wise added butyronitrile (1.0 g, 14 mmol) in THF (4 mL) and the reaction mixture was stirred at −78° C. for 1 h. This was followed by a dropwise addition of ethyl 2,2-difluoroacetate (1.6 g, 13 mmol) at −78° C. and the reaction mixture was stirred for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was cooled to 0° C. and quenched with saturated solution of ammonium chloride (5 mL) and extracted with ethyl acetate (20 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude was purified by silica gel (230-400 mesh) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound XXXa as a light brown viscous liquid. Yield: 1.2 g (57%); LC_MS Calculated. for C 6 H 6 F 2 NO is 147.11, Observed, 146.12; [M−H] − .

Step-2: 5-(difluoro methyl)-4-ethyl-1H-pyrazol-3-amine (XXXb)

To a stirred solution of 2-ethyl-4,4-difluoro-3-oxobutanenitrile XXXa (1.2 g, 8.2 mmol) in ethanol (40 mL) was added hydrazine hydrate (1:1) (0.49 g, 9.8 mmol) at room temperature and the reaction mixture was stirred at 120° C. for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, reaction mixture was cooled to room temperature and concentrated to remove the solvent. The obtained residue was dissolved in water (30 mL) and extracted with 10% methanol in DCM (50 mL×2). The combine organic layer was dried over anhydrous sodium sulphate and concentrated to get crude material XXXb. The crude was taken for next step without further purification. LCMS Calculated. for C 6 H 9 F 2 N 3 is 161.15 Observed. 160.10; [M−H] − .

Step-3: 7-amino-2-(difluoro methyl)-3-ethyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXXc)

To a stirred solution of 5-(difluoro methyl)-4-ethyl-1H-pyrazol-3-amine XXXb (0.6 g, 4 mmol) in pyridine (2 mL) was added 2-(bis(methylthio)methylene) malononitrile Ia (0.8 g, 4 mmol) and the reaction mixture was heated at 120° C. for 2.5 h (using the guard tube). The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and poured into ice cold water (100 mL). The precipitate formed was filtered and washed with cold water. The solid obtained was dried and azeotrope with toluene to yield the title compound as a pale-yellow solid. The crude was purified by silica gel (230-400) column chromatography by using 0-30% ethyl acetate in n-hexane afford the desired compound XXXc as a pale-yellow solid. Yield: 0.5 g (50%); LCMS Calculated. for C 11 H 11 F 2 N 5 S is 283.29, Observed, 284.20; [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 6.77 (s, 1H), 6.30 (s, 2H), 2.87-2.81 (m, 2H), 2.64 (s, 3H), 1.29 (t, J=8.0 Hz 3H).

Step-4: 7-amino-2-(difluoro methyl)-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXX)

To a stirred solution of 7-amino-2-(difluoro methyl)-3-ethyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXXc (0.5 g, 2 mmol) in DCM (50 ml) at 0° C. was added mCPBA (1 g, 7 mmol) slowly and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC. After completion of the reaction, the reaction mixture was quenched with aq. NaHCO 3 (50 ml) and extracted with DCM (50 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. Crude product XXX was taken for next step without further purification. Yield: 0.3 g (50%); LC_MS Calculated. for C 11 H 11 F 2 N 5 O 2 S is 315.28, Observed, 316.20; [M+H]+. 1H NMR (400 MHz, CDCl 3 ): δ 9.53 (s, 2H), 7.31 (s, 1H), 3.33 (s, 3H), 2.84-2.78 (m, 2H), 1.23 (t, J=8 Hz 3H).

Synthesis of 2-(6-(1-(methoxymethyl) cyclopropyl) pyridin-2-yl) ethan-1-amine (XXXI)

Step-1: (1-(6-bromopyridin-2-yl) cyclopropyl) methanol (XXIIa)

To a solution of ethyl 1-(6-bromopyridin-2-yl) cyclopropane-1-carboxylate XXIIb (2.2 g, 8.1 mmol) in THF (88 mL) at 0° C. was added DIBAL-H (18 mL, 1 M, 18 mmol) under N 2 atmosphere and the resulting mixture was stirred at 0° C. for 30 min. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was quenched with saturated solution of NH 4 Cl (25 mL) and added ethyl acetate (50 ml). The resulting solid was filtered through a celite bed and washed with ethyl acetate (30 mL). The filtrate was concentrated under reduced pressure to afford the desired compound XXXIa as a pale-yellow viscous liquid. Yield: 1.68 g (90%); LCMS Calculated. for C 9 H 10 BrNO is 228.08, Observed, 230.10; [M+H] + ; 1 H NMR (400 MHz, dmso-d6): δ 7.64 (t, J=8 Hz, 1H), 7.53 (d, J=8.0 Hz, 1H), 7.38 (d, J=8 Hz, 1H), 4.82 (t, J=8 Hz, 1H), 3.71 (d, J=8 Hz 2H), 1.07-1.04 (m, 2H), 0.94-0.91 (m, 2H).

Step-2: 2-bromo-6-(1-(methoxymethyl)cyclopropyl) pyridine (XXXIb)

To a stirred solution of (1-(6-bromopyridin-2-yl) cyclopropyl) methanol XXXIa (1.68 g, 7.37 mmol) in DMF (10 mL) at 0° C. under inert atmosphere was added sodium hydride (482 mg, 55% Wt., 11.0 mmol) and the resulting mixture was stirred at 0° C. for 5 min. This was followed by an addition of methyl iodide (0.55 mL, 8.84 mmol) and mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with ice cold water (20 mL) and the resulting mixture was extracted with ethyl acetate (20 mL×2). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford XXXIb as light brown viscous liquid. The crude was taken for next step without further purification. Yield: 1.79 g (99%); LCMS Calculated. for C 10 H 12 BrNO is 242.12, Observed, 242.15; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.42 (t, J=8 Hz, 1H), 7.35 (d, J=4 Hz, 1H), 7.23 (t, J=8.0 Hz, 1H), 3.65 (S, 2H), 3.4 (S, 3H), 1.63-1.31 (m, 2H),1.26-0.96 (m, 2H)

›Abbreviations · 20 of 69

Step-3: 2-(1-(methoxymethyl) cyclopropyl)-6-vinylpyridine (XXXIc)

To a solution of 2-bromo-6-(1-(methoxymethyl) cyclopropyl) pyridine XXXIb (0.8 g, 3 mmol) in 1,4-Dioxane (15 mL) was added vinyl boronic acid pinacol ester (0.6 mL, 4 mmol) and tri potassium phosphate (1 g, 7 mmol), purged with N2 gas for 15 min with vigorous stirring. This was followed by an addition of PdCl2(dppf) (0.1 g, 0.2 mmol) and heated to 120° C. for 16 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to room temperature and reaction mixture was concentrated under reduced pressure. Then added water (70 mL) and the resulting mixture was extracted with ethyl acetate (50 mL×2). The organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford a light brown viscous liquid. The crude compound was purified by combi-flash (230-400, 120 g packed) column chromatography using ethyl acetate in hexane and peak eluted with 5% ethyl acetate in hexane was concentrated to afford the desired compound XXXIc as an off-white solid. Yield: 0.42 g (70%); LCMS Calculated. for C 12 H 15 NO is 189.26, Observed, 190.25; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.55-7.51 (m, 2H), 7.06 (d, J=4 Hz, 1H), 6.74 (t, J=8 Hz, 1H), 6.18 (d, J=16 Hz 1H), 5.38 (d, J=8 Hz 1H), 3.72 (S, 2H),3.40 (S, 3H),1.35 (d, J=4 Hz, 2H), 0.93 (t, J=4 Hz, 2H),

Step-4: 6-(1-(methoxymethyl) cyclopropyl) picolinaldehyde (XXXId)

To a solution of 2-(1-(methoxymethyl) cyclopropyl)-6-vinylpyridine XXXIc (0.425 g, 2.25 mmol) in THF (9 mL) and water (20 mL), a solution of osmium tetroxide (0.06 mL, 1.12 mmol) was added, and the reaction was stirred at rt for 30 min. This was followed by an addition of sodium periodate (720 mg, 3.37 mmol) and the reaction was stirred at rt for 1 h. The mixture was diluted with water (20 mL) and extracted with ethyl acetate (20 mL×2). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and evaporated under reduced pressure to afford XXXId as brown viscous liquid. The crude was taken for next step without further purification. Yield: 290 mg (67.5%); LCMS Calculated. for C 11 H 14 NO 2 is 191.23, Observed, 192.25; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 9.97 (S, 1H), 7.76-7.71 (m, 2H), 7.63 (d, J=8.0 Hz, 1H), 3.74 (S, 2H), 3.43 (S, 3H), 1.43 (t, J=4 Hz, 2H), 1.02 (t, J=4 Hz, 2H).

Step-5: (E)-2-(1-(methoxymethyl) cyclopropyl)-6-(2-nitrovinyl) pyridine (XXXIe)

To a solution of 6-(1-(methoxymethyl) cyclopropyl) picolinaldehyde XXXId (0.6 g, 3 mmol) in DCM (12 mL) at 0° C. under inert atmosphere was added nitromethane (0.2 mL, 4 mmol), triethylamine (2 mL, 20 mmol) and the resulting mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion of (CHO—OH), DCM was evaporated under reduced pressure to afford a brown viscous liquid. To this added mesyl-Cl (0.7 mL, 9 mmol) and triethylamine (2 mL, 20 mmol) drop wise in DCM (10 mL) under N 2 gas at 0° C. The reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC analysis which indicated completion of the reaction. The reaction mixture was concentrated under reduced pressure. Added water (10 mL) and extracted with ethyl acetate (10 mL*2). The combined organic layer was given brine wash and concentrated under reduced pressure to afford XXXIe a brown viscous liquid. The crude was taken for next step without further purification. Yield: 0.7 g (99%); LCMS Calculated. for C 12 H 15 N 2 O 3 is 234.26, Observed, 235.25; [M+H] + .

Step-6: 2-(6-(1-(methoxymethyl) cyclopropyl) pyridin-2-yl) ethan-1-amine (XXXI)

To a solution of LiAlH 4 (0.06 g, 2 mmol) in dry diethyl ether (20 mL) at 0° C. under inert atmosphere was added (E)-2-(1-(methoxymethyl) cyclopropyl)-6-(2-nitrovinyl) pyridine XXXIe (0.4 g, 2 mmol) in dry diethyl ether (7 mL) and the resulting mixture was stirred at rt for 1 h. The progress of the reaction was monitored by TLC analysis. After completion of the reaction, the reaction mixture was cooled to 0° c. and quenched with water (1 mL). To the resulting mixture 15% KOH solution was added and stirred for 10 min. Added ethyl acetate (25 ml) and stirred at rt for 20 min. The resulting mixture was filtered through a celite bed and the residue was washed with ethyl acetate (50 ml×4). The combined filtrate was concentrated under reduced pressure to afford a desired compound XXXI as a pale brown viscous liquid. The crude was taken for next step without further purification. Yield: 0.34 g (99%).

Synthesis of 2-(6-(2-aminoethyl) pyridin-2-yl)-2-methylpropan-1-ol. (XXXII)

Step-1: Ethyl 2-(6-bromopyridin-2-yl)-2-methylpropanoate (XXXIIa)

A solution of ethyl 2-(6-bromopyridin-2-yl) acetate (CAS: 955369-63-8; 1 g, 4 mmol) in THF (15 mL) was cooled to 0° C. and potassium tert-butoxide (1 g, 10 mmol) was added and the resulting mixture was stirred for 30 min under N 2 atmosphere. Then methyl iodide (1 mL, 20 mmol) was drop wise added and reaction mixture was stirred at RT for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under reduced pressure and water (25 mL) was added. The solution was extracted with ethyl acetate (25 mL×2). The combined organic layer was dried over anhydrous sodium sulphate and concentrated reduced pressure to afford a light brown viscous liquid. The crude compound was subjected to silica gel (200-300) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound XXXIIa as colorless viscous liquid. Yield: 1.18 g (100%); LCMS Calculated. for C 11 H 15 BrNO 2 is 274.03, Observed, 274.15; [M+H] + .

Step-2: Ethyl 2-methyl-2-(6-vinylpyridin-2-yl) propanoate (XXXIIb)

A solution of ethyl 2-(6-bromopyridin-2-yl)-2-methylpropanoate XXXIIa (1 g, 4 mmol), potassium trifluoro(vinyl)borate (0.5 g, 4 mmol) and potassium phosphate tribasic (1 g, 6 mmol) in 1,4 dioxane (20 mL) was purged with N 2 gas for 15 min with vigorous stirring. To the reaction was added PdCl 2 (dppf) (0.1 g, 0.2 mmol) and the seal tube was closed and heated to 120° C. for 16 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to room temperature and reaction mixture was concentrated under reduced pressure. To the residue was added water (70 mL) and the resulting mixture was extracted with ethyl acetate (50 mL×2). The organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford a light brown viscous liquid. The crude compound was purified by combi-flash silica gel (230-400) column chromatography using ethyl acetate in n-hexane and the peak eluted with 5% ethyl acetate in n-hexane was concentrated to afford the desired compound XXXIIb as an off-white solid. Yield: 0.73 g (90%); LCMS Calculated. for C 13 H 17 NO 2 is 220.17, Observed, 220.20; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.59 (t, J=7.6 Hz, 1H), 7.15-7.13 (m, 2H), 6.25 (d, J=17.6 Hz, 1H), 5.42 (d, J=10.8 Hz, 1H), 4.16 (q, J=7.2 Hz, 2H), 1.57 (s, 6H), 1.18 (t, J=7.2 Hz, 3H).

›Abbreviations · 21 of 69

Step-3: ethyl 2-(6-formylpyridin-2-yl)-2-methylpropanoate (XXXIIc)

To a solution of ethyl 2-methyl-2-(6-vinylpyridin-2-yl) propanoate XXXIIb (0.73 g, 3.3 mmol) in THF (14 mL) and water (25 mL) and a solution of osmium tetroxide (680 μL, 0.33 mmol) 2.5% wt in isopropanol was added and the reaction was stirred at room temperature for 30 min. Then, Sodium metaperiodate (1.1 g, 5.0 mmol) was added and the reaction was stirred for additional 1 h. The mixture was diluted with water (15 mL) and extracted with ethyl acetate (15 mL×2). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under vacuum to afford XXXIIc the brown viscous liquid. The crude was taken for the next step without further purification. Yield: 0.503 g (68%); LCMS Calculated. for C 12 H 16 NO 3 is 222.12, Observed, 222.20; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 10.03 (s, 1H), 7.83 (d, J=3.6 Hz, 2H), 7.53 (t, J=4.4 Hz, 1H), 4.17 (q, J=7.2 Hz, 2H), 1.57 (s, 6H), 1.20 (t, J=7.2 Hz, 3H).

Step-4: Ethyl (E)-2-methyl-2-(6-(2-nitrovinyl) pyridin-2-yl) propanoate (XXXIId)

A stirred solution of ethyl 2-(6-formylpyridin-2-yl)-2-methylpropanoate XXXIIc (506 mg, 2.29 mmol) in DCM (15 mL) was cooled to 0° C. and nitromethane (168 mg, 2.74 mmol), triethylamine (1.16 g, 1.59 mL, 11.4 mmol) were added under N 2 atmosphere. The resulting mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After completion of reaction solvent was evaporated under reduced pressure to afford a brown viscous liquid. To the reaction mixture fresh DCM (10 mL) was added and was cooled to 0° C. This was followed a dropwise addition of methane sulfonyl chloride (786 mg, 6.86 mmol) under N 2 atmosphere. The reaction mixture was stirred at rt for 30 min and the progress of the reaction was monitored by TLC analysis. The reaction mixture was concentrated under reduced pressure and water (20 mL) was added. The mixture was extracted with ethyl acetate (20 mL×2). The combined organic layer was given brine wash and concentrated under reduced pressure to afford XXXIId as brown viscous liquid. The crude was taken for the next step without further purification. Yield: 0.6 g (99.3%); LCMS Calculated. for C 13 H 17 N 2 O 4 is 265.12, Observed, 265.20; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 8.03-7.89 (m, 2H), 7.75 (t, J=7.6 Hz, 1H), 7.40-7.34 (m, 2H), 4.17 (q, J=6.8 Hz, 2H), 1.58 (s, 6H), 1.20 (t, J=7.2 Hz, 3H).

Step-5: 2-(6-(2-aminoethyl) pyridin-2-yl)-2-methylpropan-1-ol (XXXII)

A solution of aluminum lithium hydride (9 mL, 1 molar, 9 mmol) in dry diethyl ether (50 mL) was cooled to 0° C. To the resulting mixture a solution of ethyl (E)-2-methyl-2-(6-(2-nitrovinyl) pyridin-2-yl) propanoate XXXIId (0.6 g, 2 mmol) in dry diethyl ether (10 mL) was added drop wise. The resulting mixture was stirred at rt for 1 h. The progress of the reaction was monitored by TLC analysis which indicated the completion of reaction. The reaction mixture was cooled to 0° C. and dropwise added ice cold water (1 mL). To the resulting mixture 15% KOH solution (5 mL) was dropwise added followed by dropwise addition of ethyl acetate (50 mL). The resulting mixture was vigorously stirred at room temperature for 15-20 min. The reaction mixture was filtered through a celite bed, and the residue was washed with fresh ethyl acetate (50 mL×3). The combined filtrate was concentrated under reduced pressure to afford a desired compound XXXII as a light brown viscous liquid. The crude was taken for the next step without further purification. Yield: 0.4 g (100%).

Synthesis of 2-(3-(amino methyl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol (XXXIII)

Step-1: Ethyl 2-(3-(hydroxymethyl)-1H-pyrazol-1-yl)-2-methylpropanoate (XXXIIIa)

A stirred solution of ethyl 2-(3-formyl-1H-pyrazol-1-yl)-2-methylpropanoate XVIIIa (1.0 g, 4.8 mmol) in ethanol (10 mL) was cooled to 0° C., and added sodium borohydride (90 mg, 2.4 mmol) in portions. The reaction mixture was stirred at 0° C. for 30 min and the progress of the reaction was monitored by TLC analysis (polar spot). The reaction mixture was diluted with water 1 mL and removed the solvent under vacuo. To the residue was added water (10 mL) and extracted with DCM (10 mL×3). The combined organic layer was dried anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude product XXXIIIa was taken for the next step without further purification. Yield: 0.9 g (90%); LCMS Calculated. for C 10 H 17 N 2 O 3 is 213.13, Observed, 213.25; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.52 (d, J=2.0 Hz, 1H), 6.28 (d, J=2.0 Hz, 1H), 4.69 (d, J=4.4 Hz, 2H), 4.16 (q, J=6.8 Hz, 2H), 2.09 (s, 1H), 1.84 (s, 6H), 1.21 (t, J=7.2 Hz, 3H).

Step-2: Ethyl 2-(3-(bromomethyl)-1H-pyrazol-1-yl)-2-methyl propanoate (XXXIIIb)

A stirred solution of ethyl 2-(3-(hydroxymethyl)-1H-pyrazol-1-yl)-2-methylpropanoate XXXIIIa (0.2 g, 0.9 mmol) in DCM (5 mL) cooled to 0° C. under inert atmosphere and dropwise added PBr 3 (0.1 mL, 1 mmol). The reaction mixture was stirred at room temperature for 12 h. The progress of the reaction was monitored by TLC analysis (less polar spot). The reaction mixture was diluted with water (10 mL) and extracted with DCM (10 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound was subjected to silica gel (230-400 mesh) combi-flash column chromatography using ethyl acetate (0-10%) in n-hexane to afford the desired compound XXXIIIb as a pale-yellow viscous liquid. Yield: 0.16 g (53%); LCMS Calculated. for C 10 H 16 BrN 2 O 2 is 275.04, Observed, 275.15; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.52 (d, J=2.0 Hz, 1H), 6.28 (d, J=2.0 Hz, 1H), 4.69 (d, J=4.4 Hz, 2H), 4.16 (q, J=6.8 Hz, 2H), 2.09 (s, 1H), 1.84 (s, 6H), 1.21 (t, J=7.2 Hz, 3H).

Step-3: Ethyl 2-(3-(azidomethyl)-1H-pyrazol-1-yl)-2-methyl propanoate (XXXIIIc)

To a stirred solution of ethyl 2-(3-(bromomethyl)-1H-pyrazol-1-yl)-2-methylpropanoate XXXIIIb (400 mg, 1.45 mmol) in DMF (4 mL) was added sodium azide (123 mg, 1.89 mmol) and the reaction mixture was heated at 50° C. for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with ice cold water (50 mL) and extracted with ethyl acetate (50 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude was purified by combi flash (230-400 mesh) using 0-2% ethyl acetate in n-hexane to afford the desired compound XXXIIIc as a pale-yellow viscous liquid. Yield: 0.17 g (49.3%); 1 H NMR (400 MHz, CDCl 3 ): δ 7.55 (d, J=2.4 Hz, 1H), 6.30 (d, J=2.0 Hz, 1H), 4.33 (s, 2H), 4.16 (q, J=6.8 Hz, 2H), 1.85 (s, 6H), 1.20 (t, J=6.8 Hz, 3H).

›Abbreviations · 22 of 69

Step-4: Ethyl 2-(3-(amino methyl)-1H-pyrazol-1-yl)-2-methylpropanoate (XXXIIId)

To a stirred solution of ethyl 2-(3-(azidomethyl)-1H-pyrazol-1-yl)-2-methylpropanoate XXXIIIc (170 mg, 0.717 mmol) in ethanol (5 mL), added Pd/C (76.3 mg, 10%, 0.717 mmol) under N 2 atmosphere. hydrogen. The reaction was evacuated and refilled with H 2 gas. The procedure was repeated for 3-4 times and stirred at room temperature under H 2 balloon pressure for 16 h. The progress of the reaction was monitored by TLC analysis (polar spot). After completion, the reaction mixture was filtered through celite bed, and the bed was washed with methanol. The combined filtrate was concentrated under reduced pressure. The crude was purified by silica gel flash (230-400 mesh) column chromatography using 0-5% methanol in DCM to afford the desired compound XXXIIId as a pale-yellow liquid. Yield: 0.15 g (99.3%); LCMS Calculated. for C 10 H 18 N 3 O 2 is 212.14, Observed, 212.30; [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.81 (d, J=2.4 Hz, 1H), 6.27 (d, J=2.4 Hz, 1H), 4.07 (q, J=7.2 Hz, 2H), 3.72 (s, 2H), 1.72 (s, 6H), 1.12 (t, J=6.8 Hz, 3H).

Step-5: 2-(3-(Amino methyl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol (XXXIII)

To a stirred solution of LAH (2.84 mL, 1 M, 2.84 mmol) in dry diethyl ether (20 mL) solution of ethyl 2-(3-(amino methyl)-1H-pyrazol-1-yl)-2-methylpropanoate XXXIIId (150 mg, 0.710 mmol) in diethyl ether (5 ML) was added dropwise at 0° C. The reaction mixture was stirred at RT for 1 h. The reaction mixture was cooled to 0° C. and quenched with water (0.15 mL), 15% NaOH (0.15 mL) and then with ethyl acetate (10 mL). The residue was filtered through a celite bed and washed with ethyl acetate (50 mL×3 times). The filtrate was concentrated under reduced pressure to get the compound XXXIII as a pale-yellow liquid. The crude was taken for the next step without further purification. Yield: 0.12 g (100%).

Synthesis of (1-(2-(2-aminoethyl) thiazol-4-yl) cyclopropyl) methanol (XXXIV)

Step-1: Ethyl 2-(2-bromothiazol-4-yl) acetate (XXXIVa)

To a stirred solution of ethyl 2-(2-aminothiazol-4-yl) acetate (CAS: 53266-94-7, 1.0 g, 5.4 mmol) in ACN (10 mL) was added tert-butyl nitrite (0.84 g, 0.97 mL, 8.2 mmol) and the reaction mixture was stirred at 60° C. for 30 min. Then copper(I) bromide (0.77 g, 5.4 mmol) was added, and the reaction was heated at 75° C. for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated, and the residue was subjected silica gel (230-400) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound as a pale-yellow viscous liquid. Yield: 0.45 g (34.0%); LCMS Calculated. for C 7 H 9 BrNO 2 S is 249.96; Observed. 250.00 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.18 (s, 1H), 4.20 (q, J=7.2 Hz, 2H), 3.81 (s, 2H), 1.28 (t, J=7.2 Hz, 3H).

Step-2: Ethyl 1-(2-bromothiazol-4-yl) cyclopropane-1-carboxylate (XXXIVb)

A stirred solution of ethyl 2-(2-bromothiazol-4-yl) acetate XXXIVa (200 mg, 0.80 mmol) in DMF (2 mL) was cooled to 0° C. and NaH (42.2 mg, 1.76 mmol) was portion wise added under inert atmosphere. This was followed by dropwise addition of 1,2-dibromoethane (330 mg, 1.76 mmol) over 10 min and the resulting mixture was stirred at room temperature for 16 h. The progress of the reaction was monitored by TLC analysis. The reaction mixture quenched with ice cold water and extracted by ethyl acetate (20 mL×3) and the combined organic layer was dried over anhydrous sodium sulphate. The solvent was concentrated under reduced pressure to afford the desired compound XXXIVb as a light brown viscous liquid. Yield: 160 mg (72%); LCMS Calculated. for C 9 H 11 BrNO 2 S is 277.97; Observed. 278.10 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.47 (s, 1H), 4.17 (q, J=7.2 Hz, 2H), 1.70-1.68 (m, 2H), 1.50-1.49 (m, 2H), 1.26 (t, J=7.2 Hz, 3H).

Step-3: Ethyl 1-(2-vinylthiazol-4-yl) cyclopropane-1-carboxylate (XXXIVc)

To a stirred solution of ethyl 1-(2-bromothiazol-4-yl)cyclopropane-1-carboxylate XXXIVb (2.2 g, 8.0 mmol) in 1, 4-dioxane (40 mL) was added trifluoro(vinyl)-14-borane, potassium salt (1.2 g, 8.8 mmol) and the resulting mixture was purged argon for 10 min. This was followed by an addition of potassium phosphate (2.5 g, 12 mmol) and the mixture was further purged for 5 min before PdCl 2 (dppf) (0.29 g, 0.40 mmol) was added. The seal tube was closed and heated at 120° C. for 3 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mass was concentrated under vacuo the residue was subjected silica gel (230-400 mesh) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound XXXIVc as a light brown liquid. Yield: 1.1 g (61%); LCMS Calculated. for C 11 H 14 NO 2 S is 224.08; Observed. 224.15 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.36 (s, 1H), 6.77 (q, J=6.8 Hz, 1H), 5.98 (d, J=17.6 Hz, 1H), 5.50 (d, J=11.2 Hz, 1H), 4.16 (t, J=7.2 Hz, 2H), 1.69-1.66 (m, 2H), 1.50-1.47 (m, 2H), 1.28-1.22 (m, 3H).

Step-4: Ethyl 1-(2-formylthiazol-4-yl) cyclopropane-1-carboxylate (XXXIVd)

A stirred solution of ethyl 1-(2-vinylthiazol-4-yl) cyclopropane-1-carboxylate XXXIVc (1.1 g, 4.9 mmol) in THF: water (2:1; 16.5 mL) was cooled to 0° C. and added osmium tetroxide (15.75 mL 4% in water, 2.5 mmol) and the reaction mixture was stirred at 0° C. for 1 h. Then sodium periodate (1.6 g, 7.4 mmol) was added, and reaction stirred was stirred at room temperature for 16 h. The progress of the reaction was monitored by TLC analysis. The reaction was quenched with water (20 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was concentrated under reduced pressure. The crude residue was subjected to silica gel (230-400) column chromatography using 0-10% ethyl acetate/n-hexane to afford the desired compound XXXIVd as a pale-yellow viscous liquid. Yield: 1.0 g (90%); 1 H NMR (400 MHz, CDCl 3 ): δ 9.92 (s, 1H), 8.04 (s, 1H), 4.20 (q, J=7.2 Hz, 2H), 1.79 (t, J=4.0 Hz, 2H), 1.67-1.62 (m, 2H), 1.28-1.22 (m, 3H).

›Abbreviations · 23 of 69

Step-5: Ethyl (E)-1-(2-(2-nitrovinyl) thiazol-4-yl) cyclopropane-1-carboxylate (XXXIVe)

To a stirred solution of ethyl 1-(2-formylthiazol-4-yl) cyclopropane-1-carboxylate XXXIVd (1.1 g, 4.9 mmol) in DCM (14 mL) were added TEA (2.0 mL, 15 mmol) and nitromethane (0.53 mL, 9.8 mmol) and the reaction mixture was stirred at room temperature for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was concentrated under vacuo. The residue was immediately dissolved in fresh DCM (14 mL) and the resulting mixture was cooled to 0° C. TEA (2.0 mL, 15 mmol) and mesyl-Cl (0.76 mL, 9.8 mmol) were added, and the reaction mixture was stirred at 0° C. for 1 h. The reaction was quenched with saturated solution of NaHCO 3 and extracted with DCM (10 mL×3). The combined organic layer was dried over sodium sulphate and concentrated under reduced pressure. The crude compound XXXIVe was taken for the next step without further purification. Yield: 1.2 g (92.3%); LCMS Calculated. for C 11 H 13 N 2 O 4 S is 269.06; Observed. 269.15 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.97 (m, 1H), 7.81 (s, J=13.2 Hz, 1H), 4.20 (q, J=7.2 Hz, 2H), 1.72 (q, J=3.6 Hz, 2H), 1.61-1.55 (m, 4H), 1.29-1.24 (m, 3H).

Step-6: (1-(2-(2-aminoethyl) thiazol-4-yl) cyclopropyl) methanol (XXXIV)

A stirred solution of ethyl (E)-1-(2-(2-nitrovinyl) thiazol-4-yl) cyclopropane-1-carboxylate XXXIVe (1.2 g, 4.5 mmol) in dry diethyl ether (25 mL) was cooled to 0° C. and LiAlH 4 solution (18 mL, 1 M, 18 mmol) was dropwise added. The reaction mixture was stirred at room temperature for 2 h. The progress of the reaction was monitored by TLC analysis. The reaction was quenched with ice cold water (1 mL), 15% NaOH solution (1 mL). The resulting mixture was stirred at 0° C. for 10 min and ethyl acetate (20 mL) was slowly added. The mixture was stirred for 10 min before it was passed through a celite bed. The bed was washed thoroughly with ethyl acetate (100 mL×3), and the combined filtrate was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the desired compound XXXIV as a pale-yellow viscous liquid. Yield: 0.79 g (78.6%).

Synthesis of 2-(3-(2-aminoethyl)-4-fluoro-1H-pyrazol-1-yl)-2-methylpropan-1-ol (XXXV)

Step-1: Ethyl 4-fluoro-1H-pyrazole-3-carboxylate (XXXVa)

To a stirred solution of ethyl 1H-pyrazole-3-carboxylate (CAS: 5932-27-4, 1 g, 7.0 mmol) in acetonitrile (15 mL) was added select fluor (4 g, 11.3 mmol) and the reaction mixture was stirred at 65° C. for 16 h under nitrogen atmosphere. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled room temperature, diluted with water (100 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude was purified by combi flash silica gel column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound XXXVa as off-white solid. Yield: 0.5 g (50%); NMR (400 MHz, CDCl 3 ): δ 10.95 (bs, 1H), 7.59 (s, 1H), 4.46-4.41 (m, 2H) 1.41 (t, J=7.2 Hz, 3H).

Step-2: (4-Fluoro-1H-pyrazol-3-yl) methanol (XXXVb)

A solution of LiAlH 4 (15.2 mL, 1 M in THF, 15.2 mmol) in diethyl ether (20 mL) was cooled to 0° C. and drop wise added a solution of ethyl 4-fluoro-1H-pyrazole-3-carboxylate XXXVa (600 mg, 3.79 mmol) in diethyl ether (5 mL) under inert atmosphere. The reaction was stirred at rt for 1 h under and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture cooled with ice bath, quenched with drop wise addition of water (3.6 mL) and 15% NaOH (0.6 mL). To the resulting mixture was added ethyl acetate (50 mL) and stirred at rt for 30 min. The mixture was filtered, and the residue was thoroughly washed with fresh ethyl acetate (50 mL×2). The combined filtrate was concentrated under reduced pressure to afford the desired compound XXXVb as a pale-yellow liquid. The crude was taken for next step without further purification Yield: 0.40 g (90%). LCMS Calculated. for C 4 H 5 FN 2 O is 116.10, Observed. 115.00; [M−H] + .

Step-3: 4-Fluoro-1H-pyrazole-3-carbaldehyde (XXXVc)

To a stirred solution of (4-fluoro-1H-pyrazol-3-yl) methanol XXXVb (400 mg, 3.45 mmol) in THF (20 mL) was added MnO 2 (15.0 g, 172 mmol) at 0° C. The resulting reaction mixture was stirred at 50° C. for 48 h under nitrogen atmosphere. The reaction was cooled to room temperature and filtered through a celite bed. The bed was washed with fresh ethyl acetate (10 mL×3). The combined filtrate was concentrated under reduced pressure to afford desired product XXXVc as a pale-yellow liquid. The crude was taken for next step without further purification. Yield: 0.39 g (99%). LC_MS Calculated. for N 2 O is 114.02, Observed. 113.10; [M−H] + .

Step-4: Ethyl 2-(4-fluoro-3-formyl-1H-pyrazol-1-yl)-2-methylpropanoate (XXXVd)

To a stirred solution of 4-fluoro-1H-pyrazole-3-carbaldehyde XXXVc (400 mg, 3.51 mmol) in DMF (10 mL) was added cesium carbonate (2.28 g, 7.01 mmol) followed by an addition of ethyl-2-bromo-2-methylpropanoate (821 mg, 4.21 mmol). The resulting reaction mixture was stirred at rt for 12 h under nitrogen atmosphere. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with ice cold water (200 mL) and extracted with ethyl acetate (100 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude material was purified by silica gel (230-400) column chromatography using 0-5% ethyl acetate in n-hexane to afford the desired compound XXXVd as a colorless liquid. Yield: 0.9 g (100%); NMR (400 MHz, CDCl 3 ): δ 9.93 (s, 1H), 7.51-7.49 (m, 1H), 4.22-4.17 (m, 2H) 1.86 (s, 6H), 1.23 (t, J=7.2 Hz, 3H).

Step-5: Ethyl (E)-2-(4-fluoro-3-(2-nitrovinyl)-1H-pyrazol-1-yl)-2-methylpropanoate (XXXVe)

To a stirred solution of ethyl 2-(4-fluoro-3-formyl-1H-pyrazol-1-yl)-2-methylpropanoate XXXVd (800 mg, 3.51 mmol) in toluene (20 mL) were added nitromethane (2.84 mL, 52.6 mmol) and ammonium acetate (405 mg, 5.26 mmol) under nitrogen atmosphere. The resulting mixture was stirred at 100° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to room temperature, diluted with 1N HCl (20 mL) and extracted with ethyl acetate (50 mL×3). The combined organic layer was dried with anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude was purified by combi flash column chromatography using 0-2% ethyl acetate in n-hexane to afford the desired compound XXXVe as an yellow solid. Yield: 0.5 g (52%); NMR (400 MHz, CDCl 3 ): δ 7.94-7.93 (m, 1H), 7.69-7.64 (m, 1H), 7.51 (d, J=5.2 Hz, 1H), 4.21-4.16 (m, 2H) 1.83 (s, 6H), 1.23 (t, J=7.2 Hz, 3H).

›Abbreviations · 24 of 69

Step-6: 2-(3-(2-Aminoethyl)-4-fluoro-1H-pyrazol-1-yl)-2-methylpropan-1-ol (XXXV)

A stirred solution of LiAlH 4 (7.37 mL, 1 M in THF, 7.37 mmol) in diethyl ether (80 mL) cooled to 0° C. and dropwise added solution of ethyl (E)-2-(4-fluoro-3-(2-nitrovinyl)-1H-pyrazol-1-yl)-2-methylpropanoate XXXVe (500 mg, 1.84 mmol) in diethyl ether (10 mL) under nitrogen atmosphere. The reaction was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. and quenched with water (0.5 mL), 15% NaOH (0.5 mL) and again water (1.5 mL) was added. To the resulting mixture was added ethyl acetate (30 mL) and stirred for 30 min. The mixture was filtered, and the residue was washed with fresh ethyl acetate (100 mL×3). The combined filtrate was concentrated under reduced pressure to afford the desired compound XXXV as pale-yellow liquid. The crude was taken for next step without further purification. Yield: 0.35 g (94%).

Synthesis of 7-amino-3-bromo-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXXVI)

Step-1: 7-amino-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXXVIa)

To a solution of 5-methyl-1H-pyrazol-3-amine (CAS: 31230-17-8, 2 g, 20 mmol) in pyridine (50 mL) was added 2-(bis(methylthio)methylene) malononitrile Ia (4 g, 20 mmol) and stirred at 120° C. for 3 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, added water (500 mL) and filtered the solid to afford the crude compound XXXVIa as a pink solid. The crude compound was taken for the next step without further purification. Yield: 3.9 g, (90.0%); LCMS Calculated. for C 9 H 9 N 5 S is 219.06; Observed: 220.25 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.68 (s, 2H), 6.25 (s, 1H), 2.52 (d, J=5.6 Hz, 3H), 2.37 (s, 3H).

Step-2: 7-amino-3-bromo-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXXVIb)

To a solution of 7-amino-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXXVIa (1.89 g, 8.62 mmol) in acetonitrile (25 mL) at 0° C. was added N-Bromo succinimide (1.89 g, 10.6 mmol) and stirred at 0° C. for 30 min. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, added water (50 mL) and extracted with ethyl acetate (2×50 mL). The combined organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure to afford the desired compound XXXVIb as a brown solid. The crude compound was taken for the next step without any further purification. Yield: 1.9 g, (74.0%); LCMS Calculated. for C 9 H 8 BrN 5 S is 296.97; Observed.: 298.15 [M+H] + . 1 HNMR (400 MHz, CDCl 3 ): δ 6.27 (s, 2H), 2.67 (s, 3H), 2.43 (s, 3H).

Step-3: 7-amino-3-bromo-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXXVI)

To a solution of 7-amino-3-bromo-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXXVIb (0.8 g, 2.68 mmol) in acetonitrile (15 mL) at 0° C. was added mCPBA (2.31 g, 13.4 mmol) and stirred at 0° C. for 30 min. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was quenched with sodium bicarbonate (100 mL) and extracted with ethyl acetate (2×15 mL). The combined organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure to afford the crude compound XXXVI as a brown solid. The crude compound was taken for the next step without further purification. Yield: 600 mg, (67.7%); LCMS Calculated. for C 9 H 8 BrN 5 O 2 S is 328.96; Observed.: 332 [M+H] + .

Synthesis of 2-(1-41-(((tert-butyldimethylsilyl) oxy) methyl) cyclopropyl) methyl)-1H-pyrazol-3-yl) ethan-1-amine (XXXVII)

Step-1: (1-(((tert-Butyldimethylsilyl) oxy) methyl) cyclopropyl) methanol (XXXVIIa)

To a stirred solution of cyclopropane-1,1-diyldimethanol (CAS: 39590-81-3, 5 g, 0.05 mol) in DCM (100 mL) was added imidazole (5 g, 0.07 mol) and stirred at room temperature for 10 min, then reaction mixture was cooled to 0° C. followed by drop wise addition of TBDMS-Cl (7 g, 0.05 mol) under nitrogen atmosphere. The reaction mixture was stirred at room temperature for 3 h and the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (50 mL) and extracted with DCM (30 mL×2). The combined organic layer was dried over anhydrous sodium sulphate. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using (0-30%) ethyl acetate in n-hexane to afford the desired compound XXXVIIa as a colorless liquid. Yield: 5.0 g (50%). 1 H NMR (400 MHz, CDCl 3 ): δ 3.61 (s, 2H), 3.57 (d, J=5.6 Hz, 2H), 2.74 (t, J=5.6 Hz, 1H), 0.91 (s, 9H), 0.53-0.50 (m, 2H), 0.47-0.43 (m, 2H). 0.07 (s, 6H).

Step-2: ((1-(Bromomethyl) cyclopropyl) methoxy) (tert-butyl) dimethyl silane (XXXVIIb)

To a stirred solution of (1-(((tert-butyldimethylsilyl) oxy) methyl) cyclopropyl) methanol XXXVIIa (4 g, 20 mmol) in DCM (100 mL) was added triphenylphosphine (7 g, 30 mmol) and stirred at room temperature for 20 min, then reaction mixture was cooled to 0° C. followed by drop wise addition of carbon tetrabromide (9 g, 30 mmol) under nitrogen atmosphere. Then reaction was stirred at room temperature for 1 h and the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (50 mL) and extracted with DCM (25 mL×3). The combined organic layer was dried over anhydrous sodium sulphate. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using (0-20%) ethyl acetate in n-hexane to afford the desired compound XXXVIIb as a colorless liquid. Yield: 4.0 g (80%). 1 H NMR (400 MHz, CDCl 3 ): δ 3.56 (s, 2H), 3.49 (s, 2H), 0.93-0.89 (m, 9H), 0.74-0.71 (m, 2H), 0.61-0.57 (m, 2H). 0.07 (s, 6H).

Step-3: 1-((1-(((tert-Butyldimethylsilyl) oxy) methyl) cyclopropyl) methyl)-1H-pyrazole-3-carbaldehyde (XXXVIIc)

›Abbreviations · 25 of 69

To a stirred solution of 1H-pyrazole-3-carbaldehyde (1 g, 10 mmol) in DMF (10 mL) was added cesium carbonate (7 g, 20 mmol) and stirred at room temperature for 20 min, then reaction mixture was cooled to 0° C. followed by drop wise addition of ((1-(bromomethyl) cyclopropyl) methoxy) (tert-butyl) dimethyl silane XXXVIIb (3 g, 10 mmol) under nitrogen atmosphere. Then reaction mixture was stirred at room temperature for 16 h and the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (50 mL) and extracted with ethyl acetate (25 mL×3). The combined organic layer was dried over anhydrous sodium sulphate. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using (0-20%) ethyl acetate in n-hexane to afford the desired compound XXXVIIc as a colorless liquid. Yield: 1.5 g (50%). LCMS Calculated. for C 15 H 26 N 2 O 2 Si is 294.17; Observed. 295.25 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 9.97 (s, 1H), 7.52 (d, J=1.6 Hz, 1H), 6.80 (d, J=2.4 Hz, 1H), 4.21 (s, 2H), 3.32 (s, 2H), 0.90 (s, 9H), 0.70-0.68 (m, 2H), 0.58-0.56 (m, 2H). 0.02 (s, 6H).

Step-4: (E)-1-((1-(((tert-Butyldimethylsilyl) oxy) methyl) cyclopropyl) methyl)-3-(2-nitrovinyl)-1H-pyrazole (XXXVIId)

To a stirred solution of 1-((1-(((tert-Butyldimethylsilyl) oxy) methyl) cyclopropyl) methyl)-1H-pyrazole-3-carbaldehyde XXXVIIc (1.5 g, 5.1 mmol) in toluene (50 mL) was added nitromethane (4.7 g, 4.1 mL, 76 mmol) and stirred at room temperature for 10 min followed by addition of ammonium acetate (0.59 g, 7.6 mmol) then reaction mixture was stirred at 100° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was cooled to room temperature, diluted with 1N HCl (50 mL) and extracted with ethyl acetate (25 mL×3). The combined organic layer was dried over anhydrous sodium sulphate. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using (0-30%) ethyl acetate in n-hexane to afford the desired compound XXXVIId as a light-yellow solid. Yield: 1.15 g (65%). LCMS Calculated. for C 16 H 27 N 3 O 3 Si is 337.18; Observed. 338.20 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.96 (d, J=13.2 Hz, 1H), 7.61 (d, J=13.2 Hz, 1H), 7.26 (s, 1H), 6.53 (s, 1H), 4.16 (s, 2H), 3.31 (s, 2H), 0.90 (s, 9H), 0.67 (m, 2H), 0.55 (m, 2H). 0.02 (s, 6H).

Step-5: 2-(1-41-(((tert-Butyldimethylsilyl) oxy) methyl) cyclopropyl) methyl)-1H-pyrazol-3-yl) ethan-1-amine (XXXVII)

To a stirred solution of lithium aluminum hydride (0.01 L, 1.0 molar in THF, 10 mmol) in diethyl ether (70 mL) was added (E)-1-((1-(((tert-Butyldimethylsilyl) oxy) methyl) cyclopropyl) methyl)-3-(2-nitrovinyl)-1H-pyrazole XXXVIId (1.15 g, 3.41 mmol) dropwise at 0° C. under nitrogen atmosphere. The reaction mixture was stirred at room temperature for 1 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. was added 1.2 mL of ice-cold water and 1.2 mL of 15% KOH solution then stirred at room temperature for another 30 min. The resulting reaction mixture was diluted with 100 mL of ethyl acetate filtered through Buchner funnel and the solid was thoroughly washed with 250 mL of ethyl acetate. The filtrate was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford a desired compound XXXVII as light-yellow liquid. The crude product was taken for the next step without further purification. Yield: 0.81 g (100%). LCMS Calculated for C 16 H 31 N 3 OSi is 309.22; Observed: 310.30 [M+H] + .

Synthesis of 2-(6-(2-aminoethyl) pyridin-2-yl)-2,2-difluoroethan-1-ol (XXXVIII)

Step-1: Ethyl 2,2-difluoro-2-(6-formylpyridin-2-yl) acetate (XXXVIIIa)

To a solution of 6-bromopicolinaldehyde (CAS: 34160-40-2, 1 g, 5 mmol) in DMSO (5 mL) were added copper (0.8 g, 0.01 mol) and ethyl 2-bromo-2,2-difluoroacetate (CAS: 667-27-6, 1 g, 7 mmol) under N 2 at room temperature in a seal tube with Teflon screw-stopper. Seal tube was closed, and reaction mixture was stirred at 100° C. for 2 h. The reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to room temperature was added 50 mL of ethyl acetate and 50 mL 1.3 molar potassium dihydrogen phosphate solution. The resulting mixture was stirred at room temperature for 30 min. The solid was filtered off and thoroughly washed with the ethyl acetate (30 mL×2). The filtrate was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using 0-6% EtOAc in n-hexane to afford the desired compound XXXVIIIa as an off-white solid. Yield: 0.93 g (80%). LCMS Calculated. for C 10 H 9 F 2 NO 3 is 229.05; Observed. 230.15 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 9.95 (bs, 1H), 8.35-8.13 (m, 3H), 4.38 (q, J=7.2 Hz, 2H), 1.25 (t, J=7.2 Hz, 3H).

Step-2: Synthesis of ethyl (E)-2,2-difluoro-2-(6-(2-nitrovinyl) pyridin-2-yl) acetate (XXXVIIIb)

To the stirred solution of ethyl 2,2-difluoro-2-(6-formylpyridin-2-yl) acetate XXXVIIIa (930 mg, 4.06 mmol) in DCM (20 mL) was added triethylamine (1.13 mL, 8.12 mmol) and nitromethane (263 μL, 4.87 mmol) at room temperature under N 2 . The resulting reaction mixture was stirred at room temperature for 2 h and the reaction was monitored by TLC analysis. After completion, DCM was concentrated to dryness and the residue was immediately taken for next in situ step. To a solution of crude ethyl 2,2-difluoro-2-(6-(1-hydroxy-2-nitroethyl) pyridin-2-yl) acetate (1.177 g, 4.06 mmol) in DCM (25 mL) was added triethylamine (1.70 mL, 12.17 mmol) and cooled to 0° C. followed by a dropwise addition of methane sulfonyl chloride (1.394 g, 1.226 mL, 12.17 mmol). The reaction was stirred at room temperature for 1 h. The progress of reaction was monitored by TLC analysis. After completion, the reaction mixture was quenched with water (25 mL) and extracted with DCM (20×2). The combined organic layer was washed with 25 mL of brine, passed through anhydrous sodium sulphate, and concentrated under reduced pressure. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using 0-6% ethyl acetate in n-hexane to afford the desired compound XXXVIIIb as a viscous brown liquid. Yield: 0.77 g (70%). LCMS Calculated. for C 11 H 10 F 2 N 2 O 4 is 272.06; Observed. 273.15 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.26-7.97 (m, 5H), 4.38 (q, J=7.2 Hz, 2H), 1.25 (t, J=4.0 Hz, 3H).

›Abbreviations · 26 of 69

Step-3: Synthesis of 2-(6-(2-Aminoethyl) pyridin-2-yl)-2,2-difluoroethan-1-ol (XXXVIII)

To a stirred solution of lithium aluminum hydride (11.3 mL, 1.0 molar in THF, 11.3 mmol) in diethyl ether (10 mL) followed by dropwise addition of solution of (E)-2,2-difluoro-2-(6-(2-nitrovinyl) pyridin-2-yl) acetate XXXVIIIb (0.770 g, 2.83 mmol) in diethyl ether (5 mL) at 0° C. under nitrogen atmosphere. The resulting mixture was stirred at room temperature for 2 h and the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. was added 0.7 mL of ice-cold water and 0.7 mL of 15% KOH solution then stirred at room temperature for another 30 min. The resulting reaction mixture was diluted with 100 mL of ethyl acetate filtered and the solid was thoroughly washed with 100 mL of ethyl acetate. The filtrate was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford a desired compound XXXVIII as light brown viscous liquid. The crude was taken for the next step without further purification. Yield: 0.56 g (98%). LCMS Calculated. for C 9 H 12 F 2 N 2 O is 202.09; Observed. 203.25 [M+H] + .

Synthesis of (2-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclobutyl) methanol (XXXIX)

Step-1: Ethyl cyclobut-1-ene-1-carboxylate (XXXIXa)

A stirred solution of DBU (54 mL, 312.3 mmol) in toluene (50 mL) was heated at 150° C. for 20 min and ethyl 1-bromocyclobutane-1-carboxylate (CAS: 35120-18-4, 5.0 g, 52.050 mmol) was added dropwise through septum under inert atmosphere. The reaction mixture was stirred at 110° C. for 3.5 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to room temperature and diluted with ice cold water (200 mL). The mixture was extracted with n-hexane (100 mL×3). The combined organic layer was washed with saturated aq. NaHSO 4 (100 mL) solution, followed by with water (100 mL). The organic layer was separated and dried over anhydrous Na 2 SO 4 , filtered, and concentrated at ambient temperature (20° C., 100 mbar vacuum). (Note: The desired product appeared to be volatile and was passing to the receiver while concentration on rotavapor; the compound). The crude compound XXXIXa (compound+some quantity of toluene) was taken for next step by assuming quantitative yield.

Step-2: Ethyl 2-(3-formyl-1H-pyrazol-1-yl) cyclobutane-1-carboxylate (XXXIXb)

A stirred solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1, 5.0 g, 52 mmol) in DMF (20 mL), was added K 2 CO 3 (14 g, 100 mmol) and the reaction mixture was cooled to 0° C. under inert atmosphere. This was followed by dropwise addition of above mixture of ethyl cyclobut-1-ene-1-carboxylate XXXIXa (16 g, 78 mmol) and toluene. The resulting mixture was stirred at rt for 48 h and progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with ice cold water (500 mL) and extracted with ethyl acetate (200 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude was purified by combi flash (230-400 silica) column chromatography using 0-5% ethyl acetate in n-hexane to afford the desired compound XXXIXb as a colorless viscous liquid. Yield: 1.4 g (10%); LCMS Calculated. for C 11 H 14 N 2 O 3 is 222.10; Observed. 223.05 [M+1] + . 1 H NMR (400 MHz, CDCl 3 ) δ 9.99 (s, 1H), 7.50 (d, J=1.6 Hz, 1H), 6.79 (d, J=2.0 Hz, 1H), 5.00 (t, J=8.8 Hz, 1H), 4.16 (q, J=7.2 Hz, 2H), 3.68-3.66 (m, 1H), 2.75-2.70 (m, 1H), 2.46-2.44 (m, 1H), 2.31-2.26 (m, 1H), 2.10-2.04 (m, 1H), 1.25 (t, J=7.2 Hz, 3H).

Step-3: Ethyl (E)-2-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclobutane-1-carboxylate (XXXIXc)

To a stirred solution of ethyl 2-(3-formyl-1H-pyrazol-1-yl) cyclobutane-1-carboxylate XXXIXb (1.4 g, 6.3 mmol) in toluene (20 mL) were added nitromethane (5.1 mL, 94 mmol) and ammonium acetate (0.73 g, 9.4 mmol) under nitrogen atmosphere. The resulting reaction mixture was stirred at 100° C. for 16 h. The reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and diluted with 1 N HCl (100 mL). The mixture was extracted with ethyl acetate (300 mL×3) and combined organic layer was dried with Na 2 SO 4 . The solution was concentrated under reduced pressure. The crude was purified by combi flash (230-400) column chromatography using 0-5% ethyl acetate in n-hexane to afford the desired compound XXXIXc as a pale-yellow viscous liquid. Yield: 1.2 g (71%); LCMS Calculated. for C 12 H 15 N 3 O 4 is 265.10; Observed. 267.30 [M+1] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.98 (d, J=13.6 Hz, 1H), 7.63 (d, J=13.6 Hz, 1H), 7.48 (s, 1H), 6.52 (d, J=2.0 Hz, 1H), 4.95 (d, J=8.4 Hz, 1H), 4.19-4.13 (m, 2H), 3.67-3.60 (m, 1H), 2.45-2.38 (m, 1H), 2.33-2.25 (m, 1H), 2.10-2.00 (m, 1H), 2.09-1.99 (m, 1H), 1.27 (t, J=7.2 Hz, 3H).

Step-4: (2-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclobutyl) methanol (XXXIX)

To a stirred solution of LiAlH 4 (18 mL, 1 molar in THF, 18 mmol) in diethyl ether (100 mL), was dropwise added a solution of ethyl (E)-2-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclobutane-1-carboxylate XXXIXc (1.2 g, 4.5 mmol) in diethyl ether (50 mL) at 0° C. under inert atmosphere. The resulting mixture was allowed to warm to rt and stirred for 1 h. The reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. and quenched with water (1.2 mL) and 15% KOH (1.2 mL). The mixture was stirred for 5 min and again added water (2.4 mL). The residue was filtered and washed with ethyl acetate (200 mL×3). The filtrate was concentrated under reduced pressure to afford the desired compound XXXIX as pale-yellow liquid. The crude product was taken for the next step without further purification. Yield: 1.1 g (88%).

Synthesis of 3-(2-aminoethyl)-1-methylpyridin-2(1H)-one (XL)

Step-1: (E)-2-methoxy-3-(2-nitrovinyl) pyridine (XLa)

To a stirred solution of 2-methoxynicotinaldehyde (CAS: 71255-09-9, 5.50 g, 40.1 mmol) in toluene (60 mL) were added ammonium acetate (4.64 g, 60.2 mmol) and nitromethane (32.4 mL, 602 mmol). The reaction was stirred at 110° C. for 2 h and progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to room temperature and diluted with water (100 mL) and the resulting mixture was extracted with ethyl acetate (200 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude was purified by flash silica gel column chromatography using 0-10% ethyl acetate in n-hexane to afford the compound as a yellow solid. Yield: 4.5 g (62%). LCMS Calculated. for C 8 H 8 N 2 O 3 is 180.05, Observed. 181.25 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 8.28-8.26 (m, 1H), 8.02-7.92 (m, 2H), 7.76-7.73 (m, 1H), 7.02-6.99 (m, 1H). 4.10 (s, 3H).

›Abbreviations · 27 of 69

Step-2: 2-(2-Methoxypyridin-3-yl) ethan-1-amine (XLb)

To a stirred solution of (E)-2-methoxy-3-(2-nitrovinyl) pyridine XLa (8.5 g, 47 mmol) in diethyl ether (170 mL) at 0° C. was dropwise added LiAlH 4 (141 mL, 1 M in THF, 140 mmol) under inert atmosphere over 20 min. The reaction was stirred at rt for 2 h and progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (17 mL) followed by with 15% NaOH solution (8.5 mL) and ethyl acetate (200 mL). The mixture was diluted with ethyl acetate (300 mL) and stirred for 20 min. The white precipitate formed was filtered and washed thoroughly with fresh ethyl acetate (300 mL×3). The combined organic layer was dried over in sodium sulphate and concentrated under vacuum to afford the compound XLb as brown liquid. Yield: 6.5 g (92%). 1 HNMR (400 MHz, CDCl 3 ): δ 8.04-8.03 (m, 1H), 7.41-7.39 (m, 1H), 6.83-6.80 (m, 1H), 3.97-3.94 (m, 5H), 2.95-2.92 (m, 2H), 2.73-2.69 (m, 2H).

Step-3: 2-(2-(2-methoxypyridin-3-yl) ethyl) isoindoline-1,3-dione (XLc)

To a stirred solution of 2-(2-methoxypyridin-3-yl) ethan-1-amine XLb (5.2 g, 34 mmol) in acetic acid (60 mL) was added isobenzofuran-1,3-dione (5.1 g, 34 mmol) at room temperature. The resulting reaction mixture was refluxed with vigorous stirring for 3 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was concentrated under vacuum. The residue was dissolved in acetic anhydride (19 mL, 20 mmol) refluxed with vigorous stirring for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to room temperature and quenched with saturated NaHCO 3 solution (200 mL). The mixture was extracted with DCM (300 mL×3) and combined organic layer was dried over anhydrous Na 2 SO 4 . The solvent was removed under reduced pressure to afford the crude compound which was subjected to combi flash column chromatography using 0-20% ethyl acetate in n-hexane to afford the desired product XLc as a yellow solid. Yield: 5.0 g (52%). LCMS Calculated. for C 16 H 14 N 2 O 3 is 282.10; Observed. 283.25 [M+H] + .

Step-4: 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione (XLd)

To a stirred solution of 2-(2-(2-methoxypyridin-3-yl) ethyl) isoindoline-1,3-dione XLc (2.5 g, 8.9 mmol) in chloroform (50 mL) was added TMS-I (6.0 mL, 44 mmol) at room temperature under inert atmosphere. The reaction mixture was stirred at 85° C. for 16 h and progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was quenched by adding methanol (5 mL) and concentrated the reaction mixture. The crude was triturated with ethanol/MTBE (1:3). The solid formed was collected by filtration and dried under vacuum to afford the desired compound XLd as a yellow solid. Yield: 1.9 g (79%). LCMS Calculated. for C 15 H 12 N 2 O 3 is 268.08; Observed. 269.05 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.95 (d, J=6.4 Hz, 1H), 7.80 (t, J=3.2 Hz, 2H) 7.71-7.69 (m, 3H), 6.67 (bs, 1H), 4.04 (bs, 2H), 3.06 (bs, 2H).

Step-5: 2-(2-(1-methyl-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione (XLe)

To a stirred solution of 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (700 mg, 5.69 mmol) in dimethoxymethane (14 mL) was added K 2 CO 3 (1.57 g, 11.4 mmol) followed by an addition of iodomethane (0.7 ml, 11.4 mmol). The resulting reaction mixture was stirred at 90° C. for 24 h under nitrogen atmosphere. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to room temperature and solid was filtered off. The filtrate was concentrated under vacuum and the residue was purified by combi flash silica gel (230-400) column chromatography using 0-20% ethyl acetate in n-hexane to afford the desired product XLe as a yellow solid. Yield: 0.58 g (74%). LCMS Calculated. for C 16 H 14 N 2 O 3 is 282.10; Observed. 283.25 [M + +1]. 1 HNMR (400 MHz, CDCl 3 ): δ 7.82-7.80 (m, 2H), 7.71-7.68 (m, 2H), 7.19-7.17 (m, 1H), 7.11-7.10 (m, 1H), 5.99 (t, J=6.8 Hz, 1H), 4.01 (t, J=6.8 Hz, 2H), 3.55 (s, 3H), 2.92 (t, J=6.4 Hz, 2H).

Step-6: 3-(2-aminoethyl)-1-methylpyridin-2(1H)-one (XL)

To a stirred solution of 2-(2-(1-methyl-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLe (580 mg, 2.05 mmol) in methanol (5 mL) was added hydrazine hydrate (0.015 mL, 3.08 mmol). The reaction was stirred at rt for 2 h under nitrogen atmosphere. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was concentrated under vacuum. Then crude was diluted with water (2 mL) and acidified with conc. HCl (pH=2). The solid formed was filtered and washed with water (2 mL). The aqueous layer was basified with 1N NaOH solution and extracted with 10% methanol in DCM (100 mL×3). The combined organic layer was dried over sodium sulphate and concentrated under vacuum to afford the desired title compound XL as a pale-yellow viscous liquid. The crude was taken for next step without further purification. Yield: 0.3 g (95%). LC_MS Calculated. for C 8 H 12 N 2 O is 152.09; Observed. 153.00 [M+H] + .

Synthesis of 3-(2-aminoethyl)-1-ethylpyridin-2(1H)-one XLI

Step-1: Synthesis of 2-(2-(1-ethyl-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione (XLIa)

To a solution of (2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (0.6 g, 2.24 mmol) in dimethoxy ethane (3 mL) was added K 2 CO 3 (0.62 g, 4.47 mmol) and iodoethane (0.7 g, 4.47 mmol). The reaction mixture was stirred at 90° C. for 24 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, reaction mixture was filtered and concentrated under reduced pressure. The crude compound was purified by silica gel (230-400 mesh) column chromatography with ethyl acetate in hexane (0-50%) to get the desired compound XLIa as a yellow solid. Yield: 0.6 g, (90.0%); LC_MS calculated for C 17 H 16 N 2 O 3 is 296.12; Observed: 297.20 [M+H] + .

›Abbreviations · 28 of 69

Step-2: 3-(2-aminoethyl)-1-ethylpyridin-2(1H)-one (XLI)

To a solution of 2-(2-(1-ethyl-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLIa (0.6 g, 2.02 mmol) in methanol (6 mL) was added hydrazine hydrate (0.15 mL, 3.04 mmol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was concentrated and added water (2 mL), acidified with Conc. HCl to pH˜2. Solids were filtered, basified by sodium hydroxide (5 mL) and extracted with ethyl acetate (50 ml×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the crude compound XLI. The crude compound was taken for the next step without further purification. Yield: 0.3 g, (89.02%); LCMS Calculated. for C 9 H 14 N 2 O is 166.11; Observed. 167.30 [M+H] + .

Synthesis of 7-amino-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XLII)

Step-1: 2-formylbutanenitrile (XLIIa)

To a solution of LiHMDS (20 g, 100 mmol) in THF (200 mL) at −78° C. was added butyronitrile (5 g, 70 mmol) and stirred at the same temperature for 1 h. This was followed by an addition of ethyl formate (6 mL, 70 mmol) and stirred for 1 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was quenched with saturated ammonium chloride (50 mL) and extracted with diethyl ether (200 mL*3). The combined organic layer was dried over anhydrous sodium sulphate, filtered and concentrated under reduced pressure to afford the crude compound XLIIa as a pale-yellow viscous liquid. The crude compound was directly taken for next step without any further purification. Yield:7.1 g, (89.0%); LCMS Calculated. for C 5 H 7 NO is 97.05; Observed. 96.00 [M+H] + .

Step-2: 4-Ethyl-1H-pyrazol-5-amine (XLIIb)

To a solution of 2-formylbutanenitrile XLIIa (7.9 g, 81 mmol) and hydrazine hydrate (1:1, 4.8 mL, 98 mmol) in ethanol (80 mL) was added acetic acid (1.6 mL, 28 mmol) and stirred at 80° C. for 20 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was concentrated, added water (100 mL) and extracted with 0.5% methanol in dichloromethane (300 mL*3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to get the crude compound. The crude compound was purified by silica gel (100-200 mesh) column chromatography with methanol in dichloromethane (0-2%) to afford the desired compound XLIIb as a pale-yellow viscous liquid. Yield: 5.0 g, (60.0%).

Step-3: 7-amino-3-ethyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XLIIc)

To a solution of 4-ethyl-1H-pyrazol-3-amine XLIIb (1 g, 9 mmol) in pyridine (10 mL) was added 2-(bis(methylthio)methylene) malononitrile Ia (2 g, 10 mmol) and stirred the reaction mixture at 120° C. for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was poured into ice-cooled water and filtered the solid. The crude compound was purified by silica gel (100-200 mesh) column chromatography with ethyl acetate in n-hexane (0-10%) to afford the desired compound XLIIc as a brownish solid. Yield: 1.5 g, (70.0%); LCMS Calculated. for C 10 H 11 N 5 S is 233.07; Observed. 232.5 [M+H] +

Step-4: 7-amino-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (XLII)

To a solution of 7-amino-3-ethyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (0.7 g, 3.00 mmol) in dichloromethane (20 mL) at 0° C. was added mCPBA (2.59 g, 15.0 mmol) and stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was cooled to 0° C. and quenched with saturated sodium bicarbonate (5 mL) and extracted with ethyl acetate (30 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated to get crude material. The crude compound was washed with pentane and stirred with methanol (2 mL) for 10 min. Solid was filtered and organic layer was concentrated to afford the desired compound XLII as an off-white solid. Yield:125 mg, (15.7%); LCMS Calculated. for C 10 H 11 N 5 O 2 S is 265.06; Observed. 266.20 [M+H] + .

Synthesis of (6-(2-aminoethyl) 164 yridine-2-yl) methanol (XLIII)

Step-1: Methyl 6-(((tert-butyldimethylsilyl) oxy) methyl) picolinate (XLIIIa)

A stirred solution of methyl 6-(hydroxymethyl) picolinate (CAS: 39977-44-1, 25 g, 0.15 mol) in DCM (500 mL) at 0° C. under inert atmosphere was added imidazole (20 g, 0.30 mol) and the resulting mixture was stirred for 10 min. This was followed by a portion wise addition of TBDMS-Cl (45 g, 0.30 mol) and the was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (500 mL) and the resulting mixture was extracted with ethyl acetate (500 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to get crude compound. The crude compound was purified by silica gel (100-200) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound XLIIIa as an off-white solid. Yield: 33 g (78.0%); LCMS Calculated. C 14 H 23 NO 3 Si for is 281.14; Observed. 282.35 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): 8.01 (d, J=7.6 Hz, 1H), 7.86 (t, J=8.0 Hz, 1H), 7.74 (d, J=8.0 Hz, 1H), 4.93 (s, 2H), 3.99 (s, 3H), 0.96 (s, 9H), 0.12 (s, 6H).

Step-2: (6-(((tert-butyldimethylsilyl) oxy) methyl) pyridin-2-yl) methanol (XLIIIb)

A stirred solution of methyl 6-(((tert-butyldimethylsilyl) oxy) methyl) picolinate XLIIIa (33 g, 0.12 mol) in THF: MeOH (525 mL; 2:1) was cooled to 0° C. and added NaBH 4 (13 g, 0.35 mol) in portions under inert atmosphere over 20 min maintaining reaction temperature below 5° C. The reaction mixture was stirred at rt for 16 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under reduced pressure and to the residue was added sat. NaHCO 3 solution (300 mL). The mixture was extracted with DCM (300 mL×3) and combined organic layer was dried over anhydrous sodium sulphate. The solution was filtered and concentrated under reduced pressure. The crude compound was purified by silica gel (100-200) column chromatography using 10-20% ethyl acetate in n-hexane to afford the desired compound XLIIIb as a colorless viscous liquid. Yield: 29 g (99.0%); LCMS Calculated. C 13 H 23 NO 2 Si for is 253.15; Observed. 254.30 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): 7.70 (t, J=7.6 Hz, 1H), 7.40 (d, J=8.0 Hz, 1H), 7.08 (d, J=8.0 Hz, 1H), 4.33 (s, 2H), 4.73 (d, J=4.8 Hz, 2H), 3.78 (t, J=4.8 Hz, 1H), 0.96 (s, 9H), 0.13 (s, 6H).

›Abbreviations · 29 of 69

Step-3: (6-(((tert-butyldimethylsilyl) oxy) methyl) pyridin-2-yl) methyl methane sulfonate (XLIIIc)

A stirred solution of (6-(((tert-butyldimethylsilyl) oxy) methyl) pyridin-2-yl) methanol XLIIIb (29 g, 0.11 mol) and TEA (24 mL, 0.17 mol) in DCM (300 mL) was cooled to 0° C. under inert atmosphere. To the resulting mixture was dropwise added mesyl-Cl (11 mL, 1.2 Eq, 0.14 mol) over 30 min and the reaction mixture was stirred at 0° C. for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was quenched with sat. NaHCO 3 solution (300 mL) and extracted with DCM (300 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated over reduced pressure to afford the desired compound XLIIIc as a pale-yellow solid. Yield: 40 g (100%); LCMS Calculated. C 14 H 25 NO 4 SSi for is 331.13; Observed. 332.30 [M+H] + .

Step-4: 2-(6-(((tert-butyldimethylsilyl) oxy) methyl) pyridin-2-yl) acetonitrile (XLIIId)

To a stirred solution of (6-(((tert-butyldimethylsilyl) oxy) methyl) pyridin-2-yl) methyl methane sulfonate XLIIIc (40 g, 120 mmol) in DMF (300 mL) was added NaCN (6.49 g, 132 mmol) and the reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (500 mL) and the resulting mixture was extracted with ethyl acetate (500 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude was purified by silica gel column chromatography using 0-15% ethyl acetate in n-hexane to afford the desired compound XLIIId as a yellow viscous liquid. Yield: 21 g (66.7%); 1 H NMR (400 MHz, CDCl 3 ): 7.75 (t, J=8.0 Hz, 1H), 7.48 (d, J=7.6 Hz, 1H), 7.29 (d, J=7.6 Hz, 1H), 4.80 (s, 2H), 3.89 (s, 2H), 0.96 (s, 9H), 0.12 (s, 6H).

Step-5: Synthesis of (6-(2-aminoethyl) pyridin-2-yl) methanol (XLIII)

To a stirred solution of 2-(6-(((tert-butyldimethylsilyl) oxy) methyl) pyridin-2-yl) acetonitrile (21 g, 80 mmol) in dry THF (250 mL) at 0° C. was added BH 3 . DMS (28.8 mL, 320 mmol) and the reaction mixture was stirred at 70° C. for 3 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. and the reaction was quenched with MeOH (15 mL), and stirred for 30 min. Then added water (20 mL) and 1 M HCl solution (15 mL) and the resulting mixture was extracted with ethyl acetate (50 mL) followed by with DCM (50 mL) to get rid of the impurities. The aqueous layer was basified with 2N NaOH solution and extracted with DCM (500 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated to afford the desired compound XLIII as a brown viscous liquid. Yield: 9.8 g (81%); LCMS Calculated. C 8 H 12 N 2 O for is 152.09; Observed. 153.00 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): 7.61 (t, J=7.6 Hz, 1H), 7.51 (d, J=7.6 Hz, 2H), 4.73 (s, 2H), 3.11 (q, J=6.4 Hz, 2H), 2.92 (q, J=6.4 Hz, 2H).

Synthesis of (1-(4-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopropyl) methanol. (XLIV)

Step-1: Methyl 1-(4-formyl-1H-pyrazol-1-yl) cyclopropane-1-carboxylate (XLIVa)

To a stirred solution of 1H-pyrazole-4-carbaldehyde (CAS: 35344-95-7; 600 mg, 6.24 mmol) in DMF (3 mL) was added K 2 CO 3 (3.45 g, 25.0 mmol) at room temperature under inert atmosphere. The reaction mixture was cooled to 0° C. and added methyl 2,4-dibromobutanoate (CAS: 29547-04-4, 2.11 g, 8.12 mmol). The reaction was stirred at rt for 16 h. Progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude was purified by combi flash silica (230-400) column chromatography by using 0-20% ethyl acetate in n-hexane to afford the desired product XLIVa as a yellow liquid. Yield: 0.9 g (75%). LCMS Calculated. for: C 9 H 10 N 2 O 3 is 194.06; Observed. 195.20 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 9.88 (s, 1H), 8.06-8.01 (m, 1H), 7.98 (s, 1H), 3.71 (s, 3H) 1.92-1.89 (m, 2H), 1.70-1.67 (m, 2H).

Step-2: Methyl (E)-1-(4-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopropane-1-carboxylate (XLIVb)

To a stirred solution of methyl 1-(4-formyl-1H-pyrazol-1-yl) cyclopropane-1-carboxylate XLIVa (800 mg, 4.12 mmol) in toluene (16 mL) were added ammonium acetate (476 mg, 6.18 mmol) and nitromethane (3.77 g, 61.8 mmol) at room temperature. The reaction mixture was stirred at 110° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with 1N HCl (20 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude was purified by flash chromatography (silica gel 230-400 mess) using 0-25% ethyl acetate in n-hexane to afford the compound XLIVb as yellow solid. Yield: 0.6 g (62%). LCMS Calculated. for C 10 H 11 N 3 O 4 is 237.07; Observed. 238.20 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.92 (d, J=13.6 Hz, 1H), 7.83 (s, 1H), 7.77 (s, 1H), 7.44 (d, 13.2 Hz, 1H), 3.71 (s, 3H) 1.92-1.88 (m, 2H), 1.68-1.58 (m, 2H).

Step-3: (1-(4-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopropyl) methanol (XLIV)

A solution LiAlH 4 (15 mL, 1M in THF, 0.02 mol) in DEE (20 mL) was cooled to 0° C. and dropwise added a solution of methyl(E)-1-(4-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopropane-1-carboxylate XLIVb (0.9 g, 4 mmol) in THF (5 mL). The reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C., quenched with water (3 mL), 15% NaOH solution (1 mL) and ethyl acetate (30 mL). The mixture was stirred at RT for 30 min and filtered and the residue was washed with fresh ethyl acetate (50 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the desired compound XLIV as a brown liquid. The crude was taken for next step without further purification. Yield: 0.6 g (75%).

›Abbreviations · 30 of 69

Synthesis of (1-(3-(2-aminoethyl)-5-methyl-1H-pyrazol-1-yl) cyclopropyl) methanol. XLV

Step-1: Ethyl 5-methyl-1H-pyrazole-3-carboxylate (XLVa)

A solution of ethyl 2,4-dioxopentanoate (CAS: 615-79-2, 5 g, 0.03 mol) in ethanol (4 mL) was cooled to 0° C. and hydrazine hydrate (2 mL, 0.03 mol, 1:1) was added dropwise at 0° C. under inert atmosphere. The reaction mixture was refluxed for 1 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under reduced pressure and the residue was dissolved in water (50 mL). The mixture was extracted with ethyl acetate (50 mL×3) and the combined organic layer was given brine wash, dried over anhydrous sodium sulphate. The solution was concentrated under reduced pressure to obtain the desired product as a pale-yellow solid. The crude was taken for the next step without further purification. Yield: 3.7 g (72%). LCMS Calculated. for C 7 H 10 N 2 O 2 is 154.07; Observed. 155.05 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 6.60 (s, 1H), 4.38 (q, J=6.8 Hz, 2H), 2.36 (s, 3H), 1.38 (t, J=6.8 Hz, 3H).

Step-2: 5-methyl-1H-pyrazole-3-carbaldehyde (XLVb)

A solution of ethyl 5-methyl-1H-pyrazole-3-carboxylate XLVa (3.6 g, 23 mmol) in dry toluene (54 mL) was cooled to −78° C. under inert atmosphere and DIBAL-H (31 mL, 1.5 molar, 47 mmol) was added dropwise at −78° C. The reaction mixture was stirred at −78° C. for 1 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (10 mL) and methanol (2 mL) at −78° C. The mixture was stirred at rt for 10-15 min and ethyl acetate (30 mL) was added. The mixture was filtered through celite bed and washed with ethyl acetate thoroughly. The combined organic layer was separated, washed brine solution, and dried over anhydrous sodium sulphate. The solution was concentrated under reduced pressure to afford the desired compound XLVb as a pale-yellow solid. The crude product was taken for the next step without further purification. Yield: 1.3 g (50%

Step-3: Methyl 1-(3-formyl-5-methyl-1H-pyrazol-1-yl) cyclopropane-1-carboxylate (XLVc)

A solution of 5-methyl-1H-pyrazole-3-carbaldehyde XLVb (1.3 g, 12 mmol) in DMF (11 mL) was cooled to 0° C. and portion wise added K 2 CO 3 (6.5 g, 47 mmol). This was followed by a dropwise addition of methyl 2,4-dibromobutanoate (2.2 mL, 15 mmol) and the reaction mixture was stirred at room temperature for 16 h. The progress of reaction was monitored by TLC analysis. After completion, cold water (50 mL) was added and extracted with ethyl acetate (50 mL×3). The combined organic layer was given brine wash, dried over anhydrous sodium sulphate, and concentrated to get crude material. The crude material was purified by Combi-flash chromatography (mesh 230-400 silica gel) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound XLVc as an off-white solid. Yield: 0.47 g, (18.7%). LCMS Calculated. for C 10 H 12 N 2 O 3 is 208.08; Observed. 209.10 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 6.90 (s, 1H), 6.56 (s, 1H), 3.72 (s, 3H), 2.32 (s, 3H), 1.96 (s, 2H), 1.72 (s, 2H).

Step-4: Methyl (E)-1-(5-methyl-3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopropane-1-carboxylate (XLVd)

To a solution of methyl 1-(3-formyl-5-methyl-1H-pyrazol-1-yl) cyclopropane-1-carboxylate XLVc (480 mg, 2.31 mmol) in toluene (20 mL) were added ammonium acetate (267 mg, 3.46 mmol) and nitromethane (1.24 mL, 23.1 mmol) under inert atmosphere. The reaction mixture was stirred at 100° C. for 16 h and the progress of the was monitored by TLC analysis. After completion, the reaction was quenched by dropwise addition of 1N HCl solution (20 mL) and the resulting mixture was extracted with ethyl acetate (20 mL×3). The combined organic phase was washed with 1N HCl solution (20 mL), followed by with brine. The organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude material was purified by Combi-flash chromatography silica gel (mesh 230-400) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired product XLVd as an off-white solid. Yield: (0.383 g, 66.14%); LCMS Calculated. for C 11 H 13 N 3 O 4 is 251.09; Observed. 252.20 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.90 (d, J=13.2 Hz, 1H), 7.55 (d, J=14.0 Hz, 1H), 6.32 (s, 1H), 3.71 (s, 3H), 2.31 (s, 3H), 1.94 (s, 2H), 1.69 (s, 2H).

Step-5: (1-(3-(2-Aminoethyl)-5-methyl-1H-pyrazol-1-yl) cyclopropyl) methanol (XLV)

A solution of LiAlH 4 (6.05 mL, 1 M in THF, 6.05 mmol) in dry diethyl ether (20 mL) was cooled to 0° C. and solution of methyl (E)-1-(5-methyl-3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopropane-1-carboxylate XLVd (380 mg, 1.51 mmol) in THF (5 mL) was added dropwise. The reaction mixture was stirred at room temperature for 2 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to 0° C. and water (0.4 mL) was added slowly. This was followed by slow addition of 15% KOH solution (0.4 mL) and water (1.2 mL). The mixture was stirred at rt for 10 minutes, ethyl acetate (50 mL) was added and stirring at rt was continued for additional 15 minutes. The mixture was passed through a celite bed and washed thoroughly with fresh ethyl acetate. The combined organic layer was dried over anhydrous sodium sulphate and concentrated to get desired product XLV as pale-yellow oil. The compound was used as such for the next step without further purification. Yield: 0.370 g (crude).

Synthesis of 3-((3-(2-aminoethyl)-1H-pyrazol-1-yl) methyl) cyclobutan-1-one. (XLVI)

Step-1: (3,3-dimethoxycyclobutyl) methyl 4-methylbenzenesulfonate (XLVIa)

To a stirred solution of (3,3-dimethoxycyclobutyl) methanol (CAS:175021-11-1, 5.0 g, 34 mmol) in DCM (100 mL) was added pyridine (8.3 mL, 1.00 mmol) and the reaction mixture was cooled to 0° C. pTSCl (7.2 g, 38 mmol) was added in portions under nitrogen atmosphere. The resulting mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was diluted with saturated NaHCO 3 solution (100 mL) and extracted with DCM (100 mL×3). Combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound was purified by combi-flash column chromatography (230-400 silica) using 0-15% ethyl acetate in n-hexane to afford the desired compound XLVIa as a colorless liquid. Yield: 8.2 g, (82%); 1 H NMR (400 MHz, CDCl 3 ): δ 7.79 (d, J=8.4 Hz, 2H), 7.34 (d, J=8.4 Hz, 2H) 4.03 (d, J=7.2 Hz, 2H), 3.11 (s, 3H), 3.06 (s, 3H), 2.45 (s, 3H), 2.39-2.36 (m, 1H), 2.27-2.23 (m, 2H), 1.83-1.78 (m, 2H).

›Abbreviations · 31 of 69

Step-2: 1-((3,3-dimethoxycyclobutyl) methyl)-1H-pyrazole-3-carbaldehyde (XLVIb)

To a stirred solution of 1H-pyrazole-3-carbaldehyde (CAS: CAS: 3920-50-1, 2.4 g, 25 mmol) in DMF (20 mL) was added cesium carbonate (16 g, 50 mmol) and the reaction mixture was cooled to 0° C. (3,3-dimethoxycyclobutyl) methyl 4-methylbenzenesulfonate XLVIa (8.3 g, 27 mmol) was added slowly under nitrogen atmosphere. The resulting reaction mixture was stirred at rt for 12 h. Progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (200 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound was purified by combi-flash column chromatography using 0-15% ethyl acetate in n-hexane to afford the desired compound XLVIb as a pale-yellow viscous liquid. Yield: 4.4 g, (78%); Chemical Formula: C 11 H 16 N 2 O 3 , 1 H NMR (400 MHz, CDCl 3 ): δ 9.96 (s, 1H), 7.42 (d, J=2.0 Hz, 1H), 6.80 (d, J=2.0 Hz, 1H) 4.29 (d, J=7.6 Hz, 2H), 3.13 (s, 6H), 2.70-2.45 (m, 1H), 2.34-2.32 (m, 2H), 1.96-1.91 (m, 2H).

Step-3: (E)-1-((3,3-dimethoxycyclobutyl) methyl)-3-(2-nitrovinyl)-1H-pyrazole (XLVIc)

To a stirred solution of 1-((3,3-dimethoxycyclobutyl) methyl)-1H-pyrazole-3-carbaldehyde XLVIb (4.4 g, 20 mmol) in toluene (50 mL) was added nitromethane (16 mL, 290 mmol) followed by an addition of ammonium acetate (2.3 g, 29 mmol). The resulting mixture was stirred at 100° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was cooled rt, diluted with 1N HCl (50 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound was purified by combi-flash column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound XLVIc as a pale-yellow solid. Yield: 3.3 g, (63%).

Step-4: 2-(1-((3,3-dimethoxycyclobutyl) methyl)-1H-pyrazol-3-yl) ethan-1-amine (XLVId)

A stirred solution of LiAlH 4 (49 mL, 1 molar in THF, 49 mmol) in diethyl ether (100 mL) was cooled to 0° C. and drop wise added a solution of (E)-1-((3,3-dimethoxycyclobutyl) methyl)-3-(2-nitrovinyl)-1H-pyrazole XLVIc (3.3 g, 12 mmol) under inert atmosphere. The resulting mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. and quenched with slowly addition of water (3.3 mL), 15% KOH solution (3.3 mL) and again added (10 mL) of water. The resulting reaction mixture was stirred at rt for 20 min and ethyl acetate (100 mL) was added. The mixture was filtered, and the residue was washed fresh ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude product XLVId was directly taken for next step without purification. Yield: 2.4 g, (crude); LCMS Calculated. for C 12 H 21 N 3 O 2 is 239.32; Observed. 240.35 [M+H] + .

Step-5: 3-((3-(2-aminoethyl)-1H-pyrazol-1-yl) methyl) cyclobutan-1-one (XLVI)

To a stirred solution of 2-(1-((3,3-dimethoxycyclobutyl) methyl)-1H-pyrazol-3-yl) ethan-1-amine XLVId (2.6 g, 11 mmol) in ethanol (50 mL) was a slowly added 2N HCl (3 mL) at 0° C. under nitrogen atmosphere. The reaction was stirred at rt for 2 h and progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under vacuum. The crude product XLVI was taken directly forward to next step without further purification. Yield:2.4 g crude

Synthesis of 2-(3-(2-aminoethyl)-1H-pyrazol-1-yl) propan-1-ol. (XLVII)

Step-1: Methyl 2-(3-formyl-1H-pyrazol-1-yl) propanoate (XLVIIa)

To a solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1, 3.0 g, 31 mmol) in DMF (30 mL) was added K 2 CO 3 (17 g, 0.12 mol) and the solution was cooled to 0° C. under inert atmosphere. Then methyl 2-bromopropanoate (CAS: 5445-17-0, 5.2 mL, 47 mmol) was added dropwise and the reaction mixture was stirred at room temperature for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with cold water (50 mL) and extracted with ethyl acetate (100 mL×3). The combined organic phase was washed with brine and dried over anhydrous sodium sulphate. The solution was concentrated under reduced pressure and the crude material was purified by combi-flash silica gel (230-400) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound XLVIIa as a colorless oil. Yield: 0.70 g, (13.0%); LCMS Calculated. for C 8 H 10 N 2 O 3 is 182.06; Observed. 183.25 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 9.98 (s, 1H), 7.59 (s, 1H), 6.86 (s, 1H), 5.19 (q, J=7.2 Hz, 1H), 3.78 (s, 3H), 1.86 (d, J=6.8 Hz, 3H).

Step-2: methyl (E)-2-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) propanoate (XLVIIb)

To a solution of methyl 2-(3-formyl-1H-pyrazol-1-yl) propanoate XLVIIa (700 mg, 3.84 mmol) was dissolved in toluene (20 mL) under inert atmosphere were added ammonium acetate (444 mg, 5.76 mmol) and nitromethane (2.07 mL, 38.4 mmol), and the resulting mixture was mixture was stirred at 100° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and quenched with 1N HCl solution (25 mL). The organic phase was separated, and the aqueous phase was extracted with ethyl acetate (100 mL×3). The combined organic layer was washed with brine, dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure. The crude material was purified by combi-flash silica gel (230-4000 column chromatography using 0-20% ethyl acetate in n-hexane to afford the desired compound XLVIIb as pale-yellow oil. Yield: 560 mg, (65.7%); LCMS Calculated. for C 9 H 11 N 3 O 4 is 225.07; Observed. 226.15 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.97 (d, J=13.6 Hz, 1H), δ 7.61, (d, J=14.0 Hz, 2H), 6.61 (s, 1H), 5.14 (q, J=7.2 Hz, 1H), 3.77 (s, 3H), 1.83 (d, J=7.2 Hz, 3H).

›Abbreviations · 32 of 69

Step-3: 2-(3-(2-aminoethyl)-1H-pyrazol-1-yl) propan-1-ol (XLVII)

A solution of LiAlH 4 (9.95 mL, 1 M in THF, 9.95 mmol) in diethyl ether (20 mL) was cooled to 0° C. under inert atmosphere and dropwise added a solution of methyl (E)-2-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) propanoate XLVIIb (560 mg, 2.49 mmol) in diethyl ether (8 mL). The reaction mixture was stirred at room temperature for 2 h and the progress of reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. and quenched with sequential dropwise addition of water (0.6 mL), 15% KOH solution (0.6 mL) and again water (1.8 mL). The reaction mixture was stirred at rt for 10 min and ethyl acetate (50 mL) was added and stirred for additional 15 min. The mixture was filtered and thoroughly washed with ethyl acetate (50 mL×3). The combined organic layer was concentrated under reduced pressure to afford the desired product XLVII as pale-yellow oil. Yield: 0.47 g, (100%); LCMS Calculated. for is 169.12; Observed. 170.35 [M+H] + .

Synthesis of (6-(2-aminoethyl)-3-fluoropyridin-2-yl) methanol (XLVIII)

Step-1: (6-bromo-3-fluoropyridin-2-yl) methanol (XLVIIIa)

To a solution of 6-bromo-3-fluoropicolinaldehyde (CAS: 885267-36-7, 2 g, 10 mmol) in Methanol (20 mL) at 0° C. was added NaBH 4 (0.4 g, 10 mmol) and stirred at the same temperature for 30 min. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, added NaHCO 3 (50 mL) and extracted with ethyl acetate (50 mL×2). The combined organic layer was washed with brine (25 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure to afford the desired product XLVIIIa as off-white solid. The crude was taken for the next step without any further purification. Yield: 1.96 g, (100.0%); LCMS calculated for C 6 H 5 BrFNO is 204.95; Observed: 206.20 [M+H] + . 1 HNMR (400 MHz, CDCl 3 ): δ 7.74-7.63 (m, 2H), 5.47 (t, J=6.4 Hz, 1H), 4.55-4.54 (m, 2H).

Step-2: 6-bromo-2-(((tert-butyldimethylsilyl) oxy) methyl)-3-fluoropyridine (XLVIIIb)

To a solution of (6-bromo-3-fluoropyridin-2-yl) methanol XLVIIIa (1.96 g, 9.51 mmol) in DCM (50 mL) was added imidazole (0.972 g, 14.3 mmol). The reaction mixture was stirred at rt for 30 min This was followed by an addition of TBDMS-Cl (1.72 g, 11.4 mmol) at 0° C. and stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, water (50 mL) was added and extracted with dichloromethane (50 mL×2). The combined organic layer was washed with brine (25 mL) dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude compound was purified by silica gel (230-400 mesh) column chromatography using ethyl acetate in n-hexane (0-15%) to afford the desired compound XLVIIIb as a colorless viscous liquid. Yield: 2.8 g, (98.0%); LCMS calculated for C 12 H 19 BrFNOSi is 319.04; Observed. 321.95 [M+H] + . 1 H NMR (400 MHz, cdcl 3 ): δ 7.41-7.38 (m, 1H), 7.28-7.24 (m, 1H), 4.82 (d, J=6.0 Hz, 2H), 0.91 (s, 9H), 0.12 (s, 6H).

Step-3: 2-(((tert-butyldimethylsilyl) oxy) methyl)-3-fluoro-6-vinylpyridine (XLVIIIc)

To a solution of 6-bromo-2-(((tert-butyldimethylsilyl) oxy) methyl)-3-fluoropyridine XLVIIIb (1.8 g, 5.6 mmol) and potassium trifluoro(vinyl)borate (1-) (0.90 g, 6.7 mmol) in 1,4-dioxane (20 mL) was added potassium phosphate, tribasic (1.8 g, 8.4 mmol) and purged with N 2 for 10 min. This was followed by an addition of PdCl 2 (dppf) (0.21 g, 0.28 mmol) and stirred at 110° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion, the reaction mixture was cooled to room temperature and added water (50 mL), extracted with ethyl acetate (50 mL×2). The combined organic layer was washed with brine (30 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure. The crude compound was purified by silica gel (230-400 mesh) column chromatography using ethyl acetate in n-hexane (0-10%) to afford the desired product XLVIIIc as a colorless viscous liquid. Yield: 2.67 g, (93.0%); LCMS calculated for C 14 H 22 FNOSi is 267.15; Observed. 268.35 [M+H] + . 1 HNMR (400 MHz, CDCl 3 ): δ 7.34-7.24 (m, 2H), 6.82-6.75 (m, 1H), 6.13 (d, J=17.6 Hz, 1H), 5.43 (d, J=10.8 Hz, 1H), 4.87 (s, 2H), 0.92 (s, 9H), 0.12 (s, 6H).

Step-4: 6-(((tert-butyldimethylsilyl) oxy) methyl)-5-fluoropicolinaldehyde (XLVIIId)

To a solution of 2-((tert-butyldimethylsilyl) oxy)-3-fluoro-6-vinylpyridine XLVIIIc (2.67 g, 10.5 mmol) in THF (25 mL) at 0° C. was added osmium (VIII) oxide (6.67 mL, 4% in water, 1.05 mmol) and the stirred at rt for 30 min. This was followed by an addition of sodium periodate (2.25 g, 10.5 mmol) and stirred at rt for 2 h. The reaction was monitored by TLC analysis. After the completion of the reaction, added water (35 mL) and extracted with ethyl acetate (35 mL×2). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure to afford the crude as a brown viscous liquid. The crude compound was purified by silica gel (230-400 mesh) column chromatography using ethyl acetate in n-hexane (0-5%) to afford the desired compound XLVIIId as a colorless viscous liquid. Yield: 1.3 g, (48.0%); LCMS calculated for C 12 H 20 FNO 2 Si is 269.12; Obs. 270.30 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 10.04 (s, 1H), 7.96 (q, J=4.8 Hz, 1H), 7.53 (t, J=8.4 Hz, 1H), 4.96 (s, 2H), 0.93 (s, 9H), 0.14 (s, 6H).

Step-5: (E)-2-(((tert-butyldimethylsilyl) oxy) methyl)-3-fluoro-6-(2-nitrovinyl) pyridine (XLVIIIe)

To a solution of 6-(((tert-butyldimethylsilyl) oxy) methyl)-5-fluoropicolinaldehyde XLVIIId (1.3 g, 4.8 mmol) in dichloromethane (15 mL) at 0° C. were added nitromethane (0.31 mL, 5.8 mmol) and triethylamine (3.4 mL, 24 mmol). The resulting mixture was stirred at rt for 3 h. The progress of the reaction was monitored by TLC analysis. After the complete consumption of the starting material, the reaction mixture was concentrated, and the residue was dissolved in fresh DCM (10 mL). The resulting mixture was cooled to 0° C. and triethyl amine (3.4 mL, 34 mmol) was added followed by a dropwise addition of solution of mesyl-Cl (1.1 mL, 14 mmol) under inert atmosphere. The reaction mixture was stirred at rt for 30 min. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was concentrated, added water (25 mL) and extracted with ethyl acetate (25 mL×2). The combined organic layer was washed with brine (50 mL) and concentrated under reduced pressure. The crude compound was purified by silica gel (230-400 mesh) column chromatography using ethyl acetate in n-hexane (0-5%) to afford the desired product XLVIIIe as a colorless viscous liquid. Yield: 0.78 g, (52.0%); LCMS calculated for C 14 H 2 FN 2 O 3 Si is 312.13; Observed. 313.30 [M+H] + 1 HNMR (400 MHz, cdcl 3 ): δ 7.97-7.90 (m, 2H), 7.44 (t, J=5.2 Hz, 2H), 4.89 (s, 2H), 0.93 (s, 9H), 0.13 (s, 6H).

›Abbreviations · 33 of 69

Step-6: (6-(2-aminoethyl)-3-fluoropyridin-2-yl) methanol (XLVIII)

To a solution of lithium aluminum (III) hydride (10 mL, 1 M, 10 mmol) in dry diethyl ether (60 mL) at 0° C. was added a solution of (E)-2-((tert-butyldimethylsilyl) oxy)-3-fluoro-6-(2-nitrovinyl) pyridine XLVIIIe (0.78 g, 2.6 mmol) in dry diethyl ether (10 mL). The resulting mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was cooled to 0° C. and added ice cold water (0.7 mL). This was followed by an addition of 15% KOH (0.7 mL) and stirred for 20 min. Added ethyl acetate (75 mL) and stirred at rt for 30 min. The reaction mixture was filtered, and the residue was washed with ethyl acetate (100 mL). Filtrate was concentrated under reduced pressure to afford the desired compound XLVIII as a pale-yellow viscous liquid. The crude was taken for the next step without any further purification. Yield: 0.4 g, (90.0%); LCMS calculated for C 8 H 11 FN 2 O is 170.09; Observed: 171.30 [M+H] + .

Synthesis of 3-(2-aminoethyl)-1-(2-methoxyethyl) pyridine-2(1H)-one (XLIX)

Step-1 2-(2-(1-(2-methoxyethyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione (XLIXa)

To a stirred solution of 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (1.2 g, 4.5 mmol) in DMF (10 mL) was cooled 0° C. and added NaH (0.13 g, 5.4 mmol). This was followed by dropwise addition of 1-bromo-2-methoxyethane (CAS:6482-24-2, 0.93 g, 6.7 mmol) and the resulting reaction mixture was stirred at room temperature for 24 hours. The progress of the reaction was monitored by TLC. The reaction was quenched with water (100 mL) and the resulting mixture was extracted with ethyl acetate (200 ml×3) and the combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude residue was subjected silica gel (230-400 mesh) column chromatography using 10-70% ethyl acetate in n-hexane to afford the desired compound XLIXa as a yellow liquid. Yield: 400 mg (27%); LCMS Calculated. for C 18 H 18 N 2 O 4 is 326.13; Observed. 327.00 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.811-7.791 (m, 2H), 7.696-7.674 (m, 2H), 7.244-7.222 (m, 1H), 7.121-7.104 (m, 1H), 5.973 (t, J=6.8 Hz, 1H), 4.099 (t, J=5.2 Hz, 2H), 4.016 (t, J=6.4 Hz, 2H), 3.663 (t, J=5.2 Hz, 2H), 3.329 (s, 3H), 2.959-2.931 (m, 2H).

Step-2: 3-(2-aminoethyl)-1-(2-methoxyethyl) pyridin-2(1H)-one (XLIX)

To a stirred solution of 2-(2-(1-(2-methoxyethyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLIXa (400 mg, 1.23 mmol) in MeOH (4 mL) was added hydrazine hydrate (61.4 mg, 1.23 mmol) and the reaction mixture was stirred at room temperature for 2 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was concentrated and was added 2 mL of water then acidified with conc. HCl (pH=2). Filtered the precipitated solids and washed with water. The filtrate was basified with 4M. NaOH solution and extracted with ethyl acetate (50 ml×3), the combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the desired compound a XLIX s a yellow liquid. Yield: 200 mg (91%); LCMS Calculated. for C 10 H 16 N 2 O 4 is 196.12; Observed. 197.30 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.257-7.212 (m, 2H), 6.110 (t, J=6.8 Hz, 1H), 4.980 (bs, 2H), 4.124 (t, J=4.8 Hz, 2H), 3.678 (t, J=4.8 Hz, 2H), 3.326 (s, 3H), 2.959 (t, J=6.8 Hz, 2H), 2.689 (t, J=6.8 Hz, 2H).

Synthesis of 7-amino-2-methyl-5-(methyl sulfonyl)-3-(trifluoromethyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (L)

Step-1: 7-amino-3-iodo-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (La)

To a stirred solution of 7-amino-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXXVIa (2.0 g, 9.00 mmol) in acetonitrile (20 mL) was added N-iodosuccinimide (2.0 g, 9.00 mmol) at 0° C. The resulting mixture was stirred at 0° C. for 5 min. The progress of the reaction was monitored by TLC analysis. After completion, precipitated solid was filtered and collected solid was washed with excess of acetonitrile (50 mL) dried under high vacuo to afford desired pure product La as a brown solid. Yield: 2.8 g (90%); LCMS Calculated. for C 9 H 8 IN 5 S is 344.95; Observed. 346.10 [M+H] + ; 1 HNMR (400 MHz, DMSO-D 6 ): δ 8.84 (bs, 2H), 2.59 (s, 3H), 2.37 (s, 3H).

Step-2: 7-amino-2-methyl-5-(methylthio)-3-(trifluoromethyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (Lb)

To a stirred solution of 7-amino-3-iodo-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile La (1.0 g, 2.897 mmol) in DMF (10 mL) were added CuI (2.43 g, 12.75 mmol), trifluoro methyl trimethyl silane (1.483 g, 10.43 mmol) and potassium fluoride (0.606 g, 10.43 mmol) at room temperature under N 2 atmosphere. The resulting mixture was stirred at 80° C. for 72 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with 35 mL of DCM and filtered. The filtrate was concentrated under reduced pressure to afford black crude semi-solid. The crude compound Lb was taken for the next step without further purification. Yield: 0.32 g (40.4%); LC-MS Calculated. for C 10 H 8 F 3 N 5 S is 287.05; Observed. 286.15 [M−H] + ; 1 HNMR (400 MHz, CDCl 3 ): δ 6.34 (bs, 2H), 2.96 (s, 3H), 2.89 (s, 3H).

Step-3: 7-amino-2-methyl-5-(methyl sulfonyl)-3-(trifluoromethyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (L)

To a stirred solution of 7-amino-2-methyl-5-(methylthio)-3-(trifluoromethyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile Lb (0.23 g, 0.801 mmol) in acetonitrile (10 mL) was added mCPBA (0.691 g, 4.00 mmol) at 0° C. under N 2 atmosphere. The resulting mixture was stirred at room temperature for 3 h. The progress of the reaction was monitored by TLC analysis. After completion, to the reaction mixture was added sat. sodium bicarbonate solution (10 mL) and filtered the precipitated solid and dried under vacuo to afford desired product L as an off-white solid. The crude compound was taken for the next step without further purification. Yield: 0.17 g (66.5%); LCMS Calculated. for C 10 H 8 F 3 N 5 O 2 S is 319.04; Observed. 318.10 [M−H] + ; 1 HNMR (400 MHz, CDCl 3 ): δ 2.77 (s, 3H), 2.23 (s, 3H).

›Abbreviations · 34 of 69

Synthesis of 7-amino-3-cyclopropyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (LI)

Step-1: 2-cyclopropyl-3-oxobutanenitrile (LIa)

To a stirred solution of LDA (9.2 mL, 2 molar, 8 mmol) in THF (30 mL) at −78° C. was drop wise added solution of 2-cyclopropylacetonitrile (CAS: 6542-60-5, 1.5 g, 18 mmol) in THF (10 mL) over 10-15 min. The resulting mixture was stirred for 1 h at the same temperature. This was followed by a dropwise addition of dry ethyl acetate (CAS: 141-78-6, 1.6 mL, 17 mmol) at −78° C. The resulting mixture was stirred at same temperature for additional 1 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was quenched with sat. NH 4 Cl solution (50 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure to afford the desired compound LIa as brown liquid. Yield: 2.0 g (86%). LCMS Calculated. for C 7 H 9 NO is 123.06; Observed: 122.25 [M−1] + .

Step-2: 4-cyclopropyl-5-methyl-1H-pyrazol-3-amine (LIb)

To a stirred solution of 2-cyclopropyl-3-oxobutanenitrile LIa (2.4 g, 19 mmol) in ethanol (20 mL) was added hydrazine hydrate (1:1, 1.4 mL, 29 mmol) under inert atmosphere and the reaction mixture was heated at 100° C. for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and concentrated under reduced pressure. The obtained residue was dissolved in water (50 mL) and extracted with 10% MeOH in DCM (50 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure to afford the desired compound LIb as a pale-yellow viscous liquid. Yield: 1.65 g (61%). LCMS Calculated. for C 7 H 11 N 3 is 137.09; Observed. 138.30 [M+H] + .

Step-3: 7-amino-3-cyclopropyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (LIc)

To a stirred solution of 4-cyclopropyl-5-methyl-4H-pyrazol-3-amine LIb (2.0 g, 15 mmol) in pyridine (10 mL) was added 2-(bis(methylthio)methylene) malononitrile Ia (2.5 g, 15 mmol). The resulting mixture was stirred at 100° C. for 3 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and poured into ice cold water (500 mL). The precipitate formed was filtered and washed with cold water (100 mL). The obtained solid was dried and under vacuum to get the title compound LIc as pale-yellow solid. Yield: 2.2 g (52%). LCMS Calculated. for C 12 H 13 N 5 S is 259.09; Observed. 260.25 [M+1] + .

Step-4:7-amino-3-cyclopropyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (LI)

A stirred solution of 7-amino-3-cyclopropyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile LIc (2.2 g, 8.5 mmol) in DCM (50 mL) at 0° C. was portion-wise added mCPBA (7.3 g, 42 mmol). The resulting reaction mixture was stirred at RT for 18 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under vacuum. The crude was diluted with saturated sodium bicarbonate solution (100 mL) and stirred for 30 min. The solid formed was filtered and washed with water (100 mL×3) followed by with n-pentane (100 mL×3). The solid was dried under vacuum to afford the compound LI as an off-white solid. Yield: 2.2 g (52%). 1 HNMR (400 MHz, DMSO-D 6 ): δ 9.06 (bs, 2H), 3.38 (s, 3H), 2.46 (s, 3H), 1.81-1.77 (m, 1H), 1.06-1.01 (m, 2H), 0.19-0.89 (m, 2H).

Synthesis of 2-(1-(3-methoxypropyl)-1H-pyrazol-5-yl) ethan-1-amine. (LII)

Step-1: 1-(3-methoxypropyl)-1H-pyrazole-5-carbaldehyde (LIIa-Peak1) and 1-(3-methoxypropyl)-1H-pyrazole-3-carbaldehyde (LIIa-Peak2)

To a solution of 1H-pyrazole-3-carbaldehyde (CAS:3920-50-1, 3 g, 30 mmol) in DMF (15 mL) was added cesium carbonate (30 g, 90 mmol) and stirred at rt for 10 min. This was followed by an addition of 1-bromo-3-methoxypropane (CAS: 36865-41-5, 6 g, 40 mmol) and stirred at rt for 3 h. The progress of the reaction was monitored by TLC analysis. The TLC and LC-MS analyses indicated formation of less polar close two spots with same molecular ion peaks (m/z=169.15) indicated formation of the regio-isomers. After the completion of the reaction, water (25 mL) was added and extracted with ethyl acetate (3×25 mL). The combined organic layer was washed with brine (20 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure to afford the crude compound as a pale-yellow solid. The crude compound was purified by silica gel (230-400 mesh) column chromatography using ethyl acetate in n-hexane (0-20%) to afford the desired products as off-white solids. The structures of the desired products (LIIa-Peak 1 and LIIa-Peak II) were confirmed by 1 H NOESY study, the fraction-1 was corresponding to LIIa-Peak 1 whereas the fraction 2 was corresponds to LIIa-Peak II.

LIIa-Peak I: Yield: 0.6 g (11.4%); LCMS Calculated. for C 8 H 12 N 2 O 2 is 168.08; Observed. 169.30 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 9.88 (s, 1H), 7.56 (s, 1H), 6.91 (d, J=4.0 Hz 1H), 4.64 (t, J=7.2 Hz, 2H), 3.36 (t, J=6.0 Hz, 2H), 3.31 (s, 3H), 2.10 (t, J=6.8 Hz, 2H).

LIIa-Peak II: Yield: 3.0 g, (57.0%); LCMS Calculated. for C 8 H 12 N 2 O 2 is 168.08; Observed. 169.15 [M+H] + 1 H NMR (400 MHz, CDCl 3 ): δ 9.96 (s, 2H), 7.45 (d, J=2.0 Hz 1H), 6.79 (d, J=2.8 Hz 1H), 4.33 (t, J=6.8 Hz, 2H), 3.33 (t, J=5.2 Hz, 5H), 2.16 (t, J=6.0 Hz, 2H).

Step-2: (E)-1-(3-methoxypropyl)-5-(2-nitrovinyl)-1H-pyrazole (LIIb)

To a solution of 1-(3-methoxypropyl)-1H-pyrazole-5-carbaldehyde (LIIa-Peak 1) (0.79 g, 4.67 mmol) in toluene (25 mL) were added ammonium acetate (0.54 g, 7.00 mmol) and nitromethane (2.52 mL, 46.7 mmol). The reaction mixture was stirred at 100° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was quenched with 1N HCl (25 mL) and extracted with ethyl acetate (3×25 mL). The combined organic layer was washed with brine (10 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure to get the crude material. The crude compound was purified by silica gel (230-400 mesh) column chromatography with ethyl acetate in n-hexane (0-20%) to afford the desired product LIIb as a pale-yellow solid. Yield: 0.51 g, (51.6%); LCMS Calculated. for C 9 H 13 N 3 O 3 is 211.09; Observed. 212.30 [M+H] + .

›Abbreviations · 35 of 69

Step-3: 2-(1-(3-methoxypropyl)-1H-pyrazol-5-yl) ethan-1-amine (LII)

To a solution of lithium aluminum hydride (9 mL, 1 M in THF, 9 mmol) in diethyl ether (20 mL) at 0° C. was added a solution of (E)-1-(3-methoxypropyl)-5-(2-nitrovinyl)-1H-pyrazole LIIb (0.5 g, 2 mmol) in diethyl ether (20 mL) and stirred at rt for 1 h. The progress of the reaction was monitored by TLC analysis. After the completion, the reaction mixture was cooled to 0° C., added water (0.5 mL), 15% KOH (0.5 mL), water (1.5 mL) and stirred for 10 min. Added ethyl acetate (50 mL) and stirred for 15 min. The solids were filtered and washed with ethyl acetate (20 mL*3). The combined organic layer was concentrated and washed with hexane to afford the desired product LII as a colorless oil. Yield:0.29 g, (70%); LCMS Calculated. for C 9 H 17 N 3 O is 183.14; Observed. 183.00 [M+H] + .

Synthesis of 2-(1-(3-methoxypropyl)-1H-pyrazol-3-yl) ethan-1-amine. (LIII)

Step-1: (E)-1-(3-methoxypropyl)-3-(2-nitrovinyl)-1H-pyrazole (LIIIa)

To a solution of 1-(3-methoxypropyl)-1H-pyrazole-3-carbaldehyde. LIIa-Peak 2 (3.5 g, 21 mmol) in toluene (100 mL) were added ammonium acetate (2.4 g, 31 mmol) and nitromethane (11.0 mL, 210 mmol) and the reaction mixture was stirred at 100° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was quenched with 1N HCl (100 mL) and extracted with ethyl acetate (100 mL*3). The combined organic layer was washed with brine (50 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure to get the crude material. The crude compound was purified by silica gel (230-400 mesh) column chromatography with ethyl acetate in n-hexane (0-20%) to afford desired product LIIIa as a pale-yellow solid. Yield: 2.72 g, (62.0%); LCMS Calc. for C 9 H 13 N 3 O 3 is 211.09; Obs. 212.30 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.97 (d, J=13.6 Hz, 1H), 7.61 (d, J=13.6 Hz, 1H), 7.43 (d, J=2 Hz, 1H), 7.26-7.23 (m, 1H), 4.28 (q, J=15.6 Hz, 2H), 3.33-3.31 (m, 5H), 2.17 (q, J=4 Hz, 2H)

Step-2: 2-(1-(3-methoxypropyl)-1H-pyrazol-3-yl) ethan-1-amine (LIII)

To a solution of lithium aluminum hydride (51 mL, 1 M in THF, 51 mmol) in diethyl ether (100 mL) at 0° C. was added a solution of (E)-1-(3-methoxypropyl)-3-(2-nitrovinyl)-1H-pyrazole LIIIa (2.7 g, 13 mmol) in diethyl ether (100 mL). The reaction mixture was stirred at rt for 1 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was cooled to 0° C., added of water (2.7 mL) followed by of 15% KOH (2.7 mL) and water (8.1 mL). The reaction mixture was stirred for 10 min. Then added ethyl acetate (150 mL) and stirred for 15 min. The solid was filtered and washed with ethyl acetate (3×50 mL). The combined organic layer was concentrated and washed with n-hexane to afford the desired product LIII as a yellow solid. Yield: 2.15 g, (92%); LCMS Calculated. for C 9 H 17 N 3 O is 183.13. Observed. 183.65 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.32-7.25 (m, 2H), 6.04 (d, J=2.0 Hz, 1H), 4.16 (t, J=4.0 Hz, 2H), 3.32-3.29 (m, 5H), 2.99-2.96 (m, 2H), 2.76 (t, J=8.0 Hz, 1H), 2.12-2.05 (m, 2H)

Synthesis of 2-(1-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopropyl) ethan-1-ol. (LIV)

Step-1: ethyl 2-cyclopropylideneacetate (LIVa)

To a solution of (1-ethoxycyclopropoxy) trimethyl silane (CAS: 27374-25-0, 10.0 g, 11.5 mL, 57.37 mmol) in toluene (100 mL) were added ethyl 2-(triphenyl-15-phosphaneylidene) acetate (CAS: 1099-45-2, 21.98 g, 63.10 mmol) and benzoic acid (7.36 g, 60.23 mmol). The reaction mixture was stirred at 90° C. for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion, the reaction mixture was cooled to rt, diluted with n-pentane, and poured onto the silica gel (100-200) bed and eluted with 0-1% Et 2 O in n-pentane to afford the desired compound as a colorless liquid. Yield: 5.3 g (73.6%).

Step-2: Ethyl 2-(1-(3-formyl-1H-pyrazol-1-yl) cyclopropyl) acetate (LIVb)

To a solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1, 2.0 g, 21 mmol) in N, N-dimethyl formamide (20 mL) was added potassium carbonate (4.3 g, 31 mmol) and the reaction mixture was stirred at rt for 20 min. This was followed by an addition of ethyl 2-cyclopropylideneacetate LIVa (5.3 g, 42 mmol) at 0° C. and the reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound was purified by silica gel (230-400 mesh) column chromatography using ethyl acetate in n-hexane (0-30%) to afford the desired product LIVb as a light-yellow color liquid. Yield: 1.46 g, (32.0%); LCMS Calculated. for C 11 H 14 N 2 O 3 is 222.1; Observed: 223.2 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 9.95 (s, 1H), 7.65 (d, J=2 Hz, 1H), 6.72 (d, J=2.4 Hz, 1H), 4.10 (q, J=7.2 Hz, 2H), 2.87 (s, 2H), 1.422-1.35 (m, 2H), 1.26-1.18 (m, 5H).

Step-3: Ethyl (E)-2-(1-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopropyl) acetate (LIVc)

To a solution of ethyl 2-(1-(3-formyl-1H-pyrazol-1-yl) cyclopropyl) acetate LIVb (1.46 g, 6.57 mmol) in toluene (50 mL) was added nitromethane (6.02 g, 98.5 mmol) and the reaction mixture was stirred at rt for 10 min. This was followed by an addition of ammonium acetate (0.76 g, 9.85 mmol) and the reaction mixture was stirred at 100° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion, the reaction mixture was cooled rt, diluted with 1N HCl (50 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 and concentrated under reduced pressure. The crude compound was purified by silica gel (230-400 mesh) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired product LIVc as a light-yellow color liquid. Yield:1.2 g, (69%). 1 H NMR (400 MHz, CDCl 3 ): δ 7.95 (d, J=13.2 Hz, 2H), 7.64-7.27 (m, 2H), 6.45 (d, J=2.4 Hz, 1H), 4.12-4.07 (m, 2H), 2.84 (s, 2H), 1.42-1.31 (m, 2H), 1.29-1.15 (m, 4H).

›Abbreviations · 36 of 69

Step-4: 7-amino-3-ethyl-5-((2-(1-(3-hydroxypropyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (LIV)

To a solution of LiAlH 4 (0.69 g, 18 mmol) in diethyl ether (100 mL) at 0° C. was added a solution of ethyl (E)-2-(1-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopropyl) acetate (1.2 g, 4.5 mmol) in diethyl ether (50 mL) and the reaction mixture was stirred at rt for 1 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was cooled to 0° C. and quenched with water (1.2 mL), 15% KOH (1.2 mL) followed by water (3.6 mL). The mixture was filtered, and the white residue was thoroughly washed with ethyl acetate (150 mL×3). The combined filtrate was concentrated under reduced pressure to afford the desired product LIV as a pale-yellow viscous liquid. Yield: 0.6 g, (67.7%); LCMS Calculated. for C 10 H 17 N 30 is 195.14; Observed. 196.50 [M+H] + .

Synthesis of 3-(2-aminoethyl)-1-(3-methoxypropyl) pyridin-2(1H)-one. (LV)

Step-1: 2-(2-(1-(3-methoxypropyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione (LVa)

To a stirred solution of 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (1.2 g, 4.5 mmol) in DMF (10 mL) was added Cs 2 CO 3 (2.2 g, 6.7 mmol) at room temperature under N 2 atmosphere. The reaction mixture was cooled to 0° C., drop wise added 1-bromo-3-methoxypropane (CAS: 36865-41-5, 0.60 mL, 5.4 mmol. The resulting reaction mixture was stirred at 25° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (2×50 mL). Combined organic layer was washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuum. The crude compound was purified by combi flash column chromatography using ethyl acetate in n-hexane (0-30%) to afford the desired compound LVa as colorless viscous liquid. Yield: 0.6 g, (40%); LCMS Calculated. for C 19 H 20 N 2 O 4 is 340.14; Observed.: 341.30 [M+H] + .

Step-2: 3-(2-aminoethyl)-1-(3-methoxypropyl) pyridin-2(1H)-one (LV)

To a stirred solution of 2-(2-(1-(3-methoxypropyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione LVa (600 mg, 1.76 mmol) in MeOH (6 mL) was added hydrazine hydrate (1:1, 107 μL, 2.12 mmol). The reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated, and crude was diluted with water (2 mL). The mixture was acidified with conc. HCl (pH=2). Solid formed was filtered and washed by water. The aqueous layer was basified (pH=9). with 1N NaOH solution and extracted with ethyl acetate (3×50 mL). Combined organic layer was dried over anhydrous sodium sulphate and concentrated under vacuum to afford the desired compound LV as a colorless viscous liquid. The crude was as such taken for next step without further purification. Yield: 350 mg, (94.4%); LCMS Calculated. for C 11 H 18 N 2 O 2 is 210.14; Observed.: 211.30 [M+H] + .

Synthesis of (2-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopentyl) methanol. (LVI)

Step-1: methyl 2-(3-formyl-1H-pyrazol-1-yl) cyclopentane-1-carboxylate (LVIa)

To stirred solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1, 2 g, 20 mmol) in DMF (15 mL) was added potassium carbonate (6 g, 40 mmol) under N 2 and stirred at rt for 10 min under inert atmosphere. To the resulting mixture was added methyl cyclopent-1-ene-1-carboxylate (CAS: 25662-28-6, 3 g, 20 mmol) and stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (30 mL) and extracted with ethyl acetate (30 mL×3). The combine organic layer was washed with brine (50 mL), dried over anhydrous sodium sulphate, and concentrated under vacuum to afford pale-yellow solid. The crude was purified by combi flash silica gel (230-400) column chromatography using 0-15% ethyl acetate in n-hexane to afford the desired compound LVIa as a colorless viscous oil. Yield: 2.32 g (50%); LCMS Calculated. for C 11 H 14 N 2 O is 222.10; Observed. 223.25 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 9.95 (s, 1H), 7.51 (d, J=2 Hz, 1H), 6.77-6.76 (m, 1H), 5.00 (q, J=8.0 Hz, 1H), 3.68 (s, 3H), 3.42-3.32 (m, 1H), 2.35-2.08 (m, 3H), 2.06-1.26 (m, 3H).

Step-2: Methyl (E)-2-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopentane-1-carboxylate (LVIb)

To a stirred solution of methyl 2-(3-formyl-1H-pyrazol-1-yl) cyclopentane-1-carboxylate LVIa (2.32 g, 10.4 mmol) in toluene (75 mL) were added ammonium acetate (1.21 g, 15.7 mmol) and nitromethane (5.63 mL, 104 mmol) under inert atmosphere. The resulting mixture was stirred at 100° C. for 16 h. The progress of reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with 1N HCl solution (75 mL) and extracted with ethyl acetate (50 mL×3). The combine organic layer was washed with brine (100 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure to get crude material. The crude was purified by Combi-flash chromatography (230-400 silica gel) column chromatography using 0-15% ethyl acetate in n-hexane to afford the desired compound LVIb as a pale-yellow solid. Yield: 1.6 g (58%); LCMS Calculated. for C 12 H 15 N 3 O 4 is 265.10; Observed.: 266.25 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.96 (d, J=13.2, Hz 1H), 7.60 (d, J=13.6 Hz, 1H), 7.50 (d, J=1.6 Hz, 1H), 6.50 (d, J=2.0 Hz, 1H), 4.95-4.93 (m, 1H), 3.69 (s, 3H), 3.30-3.28 (m, 1H), 2.30-2.21 (m, 3H), 2.03-1.85 (m, 3H).

Step-3: (2-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopentyl) methanol (LVI)

A stirred solution of LiAlH 4 (30 mL, 1 M in THF, 30 mmol) in diethyl ether (80 mL) was cooled to 0° C. and drop wise added a solution of methyl (E)-2-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclopentane-1-carboxylate LVIb (1.6 g, 6.0 mmol) in THF (10 mL) under inert atmosphere. The resulting mixture was stirred at room temperature for 1.5 h. The progress of reaction was monitored by TLC analysis. After completion, reaction mixture was cooled to 0° C., quenched by dropwise addition of water (1.6 mL). This was followed by addition of 15% KOH solution (1.6 mL) and again water (4.8 mL). Then ethyl acetate (50 mL) was added to the resulting mixture and stirred for 20 minutes at rt. The mixture was filtered and solid was washed with fresh ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated to afford the desired compound LVI as a pale-yellow thick oil. The crude material was washed with n-hexane, dried under vacuo, and used for next step without purification. Yield: 1.25 g (99%); LCMS Calculated. for C 11 H 19 N 3 O is 209.15; Observed.: 210.30 [M+H] + .

›Abbreviations · 37 of 69

Synthesis of (4-(3-(2-aminoethyl)-1H-pyrazol-1-yl) tetrahydrofuran-3-yl)methanol. LVII

Step-1: methyl 4-hydroxytetrahydrofuran-3-carboxylate (LVIIa)

To a solution of methyl 4-oxotetrahydrofuran-3-carboxylate (CAS: 57595-23-0, 25 g, 0.173 mol) in THF: MeOH (2.5 L; 1:3) and the solution was cooled to 0° C. under inert atmosphere. To this solution, sodium borohydride (3.25 g, 0.087 mol) was added portion wise. The reaction mixture was stirred at 0° C. for 1 h and the progress of reaction was monitored by TLC analysis. After completion, the reaction was quenched by slow addition of water (400 mL). The resulting mixture was extracted with chloroform (700 mL×3). The combined organic layer dried over anhydrous sodium sulphate, filtered, and concentrated under vacuo to afford the desired compound LVIIa as a colorless oil. The crude was taken for the next step without purification (product is active for KMnO 4 stain). Yield: 21 g (83.3%).

Step-2: methyl 4-(tosyloxy) tetrahydrofuran-3-carboxylate (LVIIb)

To a stirred solution of methyl 4-hydroxytetrahydrofuran-3-carboxylate LVIIa (43 g, 0.294 mmol) in DCM (800 mL) were added pyridine (71 mL, 0.882 mol) and 4-methylbenzenesulfonyl chloride (168.15 g, 0.882 mol) under N 2 atmosphere. The reaction mixture was stirred at rt for 48 h and the progress of the reaction was monitored by TLC analysis. After completion, water (250 mL) was added, and the resulting mixture was extracted with DCM (300 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under vacuum to get the light brown oil. The crude was subjected to silica gel (60-120) column chromatography using 0-15% ethyl acetate in n-hexane to afford the desired compound LVIIb as a pale-yellow oil. Yield: 80 g (90.6%); Note: Tosyl elimination product also forms along with the expected product. The mixture was taken as such for the next step as both leads to the formation of desired product. LCMS Calculated for C 13 H 16 O 6 S is 300.06; Observe: 301.25 [M+H] + .

Step-3: Methyl 4-(3-formyl-1H-pyrazol-1-yl) tetrahydrofuran-3-carboxylate (LVIIc)

To a solution of 1H-pyrazole-3-carbaldehyde (26 g, 0.270 mol) in DMF (1.2 L) under N 2 was added potassium carbonate (74 g, 0.535 mmol) and the resulting mixture was stirred at rt for 10 min. Methyl 4-(tosyloxy) tetrahydrofuran-3-carboxylate LVIIb (83 g, 0.276 mol) was added drop wise, and the reaction was stirred at rt for 16 hours under N 2 atmosphere. The progress of the reaction was monitored by TLC analysis. After completion, water (1.5 L) was added, and the mixture was extracted with ethyl acetate (700 mL×3). The combined organic layer washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford the crude as brown viscous liquid. The crude was subjected to silica gel column chromatography (60-120 mesh) using 0-15% ethyl acetate in n-hexane to afford the desired compound LVIIc as a pale-yellow viscous liquid. Yield: 35 g (50.7%). LCMS Calculated. for C 10 H 12 N 2 O 4 is 224.07; Observed.: 225.25 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 9.97 (s, 1H), 7.57 (d, J=2.0 Hz, 1H), 6.82 (d, J=2.4 Hz, 1H), 5.37-5.33 (m, 1H), 4.44 (t, J=8.4 Hz, 1H), 4.28-4.17 (m, 2H), 4.12-4.08 (m, 1H), 3.86-3.77 (m, 3H), 3.65-3.60 (m, 1H).

Step-4: Methyl (E)-4-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) tetrahydrofuran-3-carboxylate (LVIId)

To a solution of methyl 4-(3-formyl-1H-pyrazol-1-yl) tetrahydrofuran-3-carboxylate LVIIc (28 g, 0.127 mol) toluene (750 mL) were added ammonium acetate (14.7 g, 0.191 mol) and nitromethane (68.4 mL, 1.27 mol). The reaction mixture was stirred at 100° C. for 16 h under inert atmosphere. The progress of the reaction was monitored by TLC analysis and complete consumption of the starting material was confirmed by using 2,4 DNP stain. After completion, the reaction mixture was cooled to rt and quenched with 1N HCl (750 mL) and the organic layer was separated. The aqueous layer was extracted with ethyl acetate (500 mL×3). The combined organic layer was given brine wash, dried over anhydrous sodium sulphate, and concentrated under reduced pressure. The crude material was purified by silica gel (mesh 60-120) column chromatography using 0-15% ethyl acetate in n-hexane to afford the desired compound LVIId as a pale-yellow oil. Yield: 19.6 g (57.8%). LCMS Calculated. for C 11 H 13 N 3 O 5 is 267.08; Observed. 268.25 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 9.96 (t, J=6.4 Hz, 1H), 7.63-7.55 (m, 2H), 6.55 (d, J=2.4 Hz, 1H), 5.31-5.27 (m, 1H), 4.48-4.39 (m, 1H), 4.27-4.22 (m, 1H), 4.16-4.06 (m, 2H), 3.75 (s, 3H), 3.61-3.56 (m, 1H).

Step-5: (4-(3-(2-aminoethyl)-1H-pyrazol-1-yl) tetrahydrofuran-3-yl) methanol (LVII)

A solution of LiAlH 4 (365 mL, 1 M in THF, 0.365 mol) in diethyl ether (1000 mL) was cooled to 0° C. under inert atmosphere. To resulting mixture was drop wise added a solution methyl (E)-4-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) tetrahydrofuran-3-carboxylate LVIId (19.5 g, 0.073 mol) in diethyl ether (200 mL) over period of 15 min. The reaction mixture was stirred at room temperature for 1.5 h. The progress of the reaction was monitored by TLC analysis (ninhydrin stain). After completion, the reaction mixture was cooled to 0° C. and water (19.5 mL) was added slowly. This was followed a slow addition of (19.5 mL) of 15% KOH solution. Again water (58.5 mL) was added slowly the mixture was stirred at rt for 10 min. To this was added ethyl acetate (500 mL) and stirred for additional 30 min. The mixture was filtered, and the residue was washed thoroughly with ethyl acetate (500 mL). The residue was transferred back to reaction flask and stirred with fresh ethyl acetate (500 mL) for 10 min and filtered. The procedure was repeated for 3-4 times and the combined filtrate was dried over anhydrous sodium sulphate. The solvent was removed under reduced pressure to afford the desired product LVII a light brown viscous liquid. Yield: 13.9 g (90.2% crude). LCMS Calculated for C 10 H 17 N 3 O 2 is 211.13; Observe 212.30 [M+H] + .

›Abbreviations · 38 of 69

Synthesis of (3-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclobutyl) methanol. (LVIII)

Step-1: Methyl 3-(tosyloxy) cyclobutane-1-carboxylate (LVIIIa)

To a stirred solution of methyl 3-hydroxycyclobutane-1-carboxylate (CAS: 4934-99-0, 5 g, 40 mmol) in DCM (30 mL) was added pyridine (9 mL, 100 mmol) under inert atmosphere. The reaction mixture was cooled to 0° C. and portion wise added 4-methylbenzenesulfonyl chloride (10 g, 60 mol). The resulting mixture was stirred at rt for 12 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (30 mL) and extracted with DCM (35 mL×2). The combined organic layer was washed with brine (20 mL), dried over anhydrous sodium sulphated and concentrated under reduced pressure. The crude product was purified by combi-flash column chromatography by eluting with ethyl acetate in n-hexane (0-15%). The peak was eluted at 10% ethyl acetate in n-hexane was concentrated to afford the desired compound LVIIIa as colorless viscous liquid. Yield: δ g (70%). LCMS Calculated. for C 13 H 16 O 5 S is 284.07; Observed: 285 [M+H] + . δ 7.78 (d, J=8.0 2H), 7.34 (d, J=8.0 2H), 4.75-4.71 (m, 1H), 3.66 (s, 3H), 2.62-2.60 (m, 1H), 2.51-2.39 (m, 7H).

Step-2: Methyl 3-(3-formyl-1H-pyrazol-1-yl) cyclobutane-1-carboxylate (LVIIIb)

To a stirred solution of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1, 1.5 g, 16 mmol) in DMF (30 mL) were added cesium carbonate (10 g, 31 mmol) and methyl 3-(tosyloxy) cyclobutane-1-carboxylate LVIIIa (5.8 g, 20 mmol) under nitrogen. The resulting reaction mixture was stirred at rt for 24 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was diluted with water (50 mL) and extracted with ethyl acetate (75 mL×2). The combined organic layer was washed with brine (35 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure. The crude product was purified by combi-flash silica gel (100-200) column chromatography using 0-20% ethyl acetate in n-hexane to afford the desired compound LVIIIb as a colorless viscous liquid. Yield: 1.7 g (52%). LCMS Calculated. for C 10 H 2 N 2 O 3 is 208.08; Observed. 209.25 [M+H] + .

Step-3: Methyl (E)-3-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclobutane-1-carboxylate (LVIIIc)

To a stirred solution of methyl 3-(3-formyl-1H-pyrazol-1-yl) cyclobutane-1-carboxylate LVIIIb (1.4 g, 6.7 mmol) in toluene (15 mL) were added ammonium acetate (0.78 g, 10 mmol) and nitromethane (5.4 mL, 10 mmol) under nitrogen. The resulting mixture was stirred at 100° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was cooled to room temperature diluted with water (15 mL). The resultant mixture was extracted with ethyl acetate (20 mL×2) and combined organic layer was washed with brine. The organic layer was dried over anhydrous sodium sulphate, concentrated under reduced pressure. The crude compound was purified by silica gel (100-200; 250 g) combi flash column chromatography using methanol in DCM (0-0.5%) to afford the desired compound LVIIIc as a colorless viscous liquid. Yield: 0.35 g (21%). LCMS Calculated. for C 11 H 13 N 3 O 4 is 251.09; Observed.: 252.25 [M+H] + .

Step-4: (3-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclobutyl) methanol (LVIII)

A stirred solution of LiAlH 4 (5.6 mL, 1 M in THF, 5.6 mmol) in diethyl ether (100 mL), was cooled to 0° C. and dropwise added a solution of methyl (E)-3-(3-(2-nitrovinyl)-1H-pyrazol-1-yl) cyclobutane-1-carboxylate (0.35 g, 1.4 mmol) under nitrogen atmosphere. The reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C., quenched with water (0.35 mL), 15% KOH (0.35 mL) and again with water (1.2 mL). The resulting reaction mixture was filtered and washed with ethyl acetate (50 mL×3). The combined filtrate was concentrated under reduced pressure to afford the compound LVIII as colorless viscous liquid. The crude was directly taken for next step without purification. Yield: 0.2 g (70%). LCMS Calculated. for C 10 H 17 N 3 O is 195.13; Observed. 196.45 [M+H] + .

Synthesis of tert-butyl (2-(3-(2-aminoethyl)-2-oxopyridin-1(2H)-yl) ethyl) carbamate (LIX)

Step-1: tert-butyl (2-(3-(2-(1,3-dioxoisoindolin-2-yl) ethyl)-2-oxopyridin-1(2H)-yl) ethyl) carbamate (LIXa)

To a stirred solution of 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (0.8 g, 3 mmol) in DMF (7 mL) was added cesium carbonate (1.0 g, 4.00 mmol) and tert-butyl (2-bromoethyl) carbamate (CAS: 39684-80-5, 0.8 g, 4.00 mmol) and the reaction mixture was stirred at room temperature for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (10 mL) and the resulting mixture was extracted with ethyl acetate (50 mL×3). The combined organic layer was dried over sodium sulphate and concentrated under reduced pressure. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using (10-50%) ethyl acetate in n-hexane to afford the desired compound LIXa as a yellow liquid. Yield: 0.520 g (50%). LCMS Calculated. for C 22 H 25 N 3 O 5 is 411.17; Obs. 412.30 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 8.01-8.00 (m, 1H), 7.84-7.82 (m, 2H), 7.72-7.70 (m, 2H), 7.40 (d, J=6.4 Hz, 1H), 6.79-6.76 (m, 1H), 4.38 (s, 2H), 3.96 (t, J=7.6 Hz, 2H), 3.57 (s, 2H), 2.95 (t, J=9.2 Hz, 2H), 1.54-1.39 (m, 9H).

Step-2: tert-butyl (2-(3-(2-aminoethyl)-2-oxopyridin-1(2H)-yl) ethyl) carbamate (LIX)

To a stirred solution of tert-butyl (2-(3-(2-(1,3-dioxoisoindolin-2-yl) ethyl)-2-oxopyridin-1(2H)-yl) ethyl) carbamate LIXa (0.52 g, 1.3 mmol) in MeOH (4 mL) was added hydrazine hydrate (1:1; 0.15 mL, 1.5 mmol) and the reaction mixture was stirred at room temperature for 2 h. The progress of the reaction was monitored by TLC analysis. After completion of the reaction, MeOH was concentrated and was added 2 mL of water then acidified with conc. HCl (pH=2). Filtered the precipitated solid and washed with water. The filtrate was basified with 4M. NaOH solution and extracted with 10% MeOH/DCM (100 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the desired compound LIX as a yellow liquid. Yield: 0.260 g (72.22%). LCMS Calculated. for C 14 H 23 N 3 O 3 is 281.17; Observed. 282.30 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.24 (d, J=6.4 Hz, 1H), 7.16 (d, J=6.4 Hz, 1H), 6.85 (bs, 1H), 6.13 (t, J=6.4 Hz, 1H), 5.14 (bs, 1H), 4.02 (t, J=5.2 Hz, 2H), 3.48-3.45 (m, 2H), 2.97 (t, J=6.8 Hz, 2H), 2.69 (t, J=6.8 Hz, 2H), 1.42 (s, 9H).

›Abbreviations · 39 of 69

Synthesis of 3-(2-aminoethyl)-1-((3-hydroxycyclobutyl) methyl) pyridine-2(1H)-one. (LX)

Step-1: 2-(2-(1-((3-((tert-butyl diphenyl silyl) oxy) cyclobutyl) methyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione (LXa)

A stirred solution of 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (1.2 g, 4.5 mmol) in DMF (15 mL) was cooled to 0° C. and added Cs 2 CO 3 (1.7 g, 5.4 mmol) followed by an addition of (3-((tert-butyldiphenylsilyl) oxy) cyclobutyl) methyl 4-methylbenzenesulfonate (CAS: 1356924-73-6, 3.1 g, 6.3 mmol). The reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was quenched with water (25 mL) and extracted with ethyl acetate (50 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude was purified by combi flash column chromatography (silica gel 230-400) eluted with 0-50% ethyl acetate in n-hexane to afford the desired compound LXa as a brown liquid. Yield: 1.1 g (42%). LCMS Calculated. for C 36 H 38 N 2 O 4 Si is 590.26; Observed.: 591.40 [M+H] + .

Step-2: 3-(2-aminoethyl)-1-((3-hydroxycyclobutyl) methyl) pyridin-2(1H)-one (LX)

To a stirred solution of 2-(2-(1-((3-((tert-butyldiphenylsilyl) oxy) cyclobutyl) methyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione LXa (1.1 g, 1.9 mmol) in MeOH (25 mL) was added hydrazine hydrate (1:1, 0.2 mL, 2.8 mmol) drop wise under N 2 atmosphere. The resulting reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was concentrated under vacuum. The crude was diluted with water (2 mL), acidified with conc. HCl (pH=2). The solid formed was filtered and washed with water. The combined filtrate was basified with 10% NaOH solution, extracted with 10% methanol in DCM (200 mL×3). The combined organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure to afford the desired compound LX as light brown viscous liquid. Yield: 0.380 g (92%). LCMS Calculated. for C 12 H 18 N 2 O 2 is 222.29; Observed. 223.30 [M+H] + .

Synthesis of tert-butyl (3-(3-(2-aminoethyl)-2-oxopyridin-1(2H)-yl) propyl) carbamate. (LXI)

Step-1: tert-butyl (3-(3-(2-(1,3-dioxoisoindolin-2-yl) ethyl)-2-oxopyridin-1(2H)-yl) propyl) carbamate (LXIa)

To a solution of 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (0.6 g, 2.24 mmol) in DMF (6 mL) at rt was added Cs 2 CO 3 (0.874 g, 2.68 mmol) followed by tert-butyl (3-bromopropyl) carbamate (CAS: 83948-53-2, 0.639 g, 2.68 mmol) and the reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion, added water (20 mL) and extracted with ethyl acetate (25 mL×2). The combined organic layer was washed with brine (10 mL×2), dried over anhydrous sodium sulphate, and concentrated under reduced pressure. The crude compound was purified by silica gel (100-200 mesh) with ethyl acetate in n-hexane (0-50%) to afford the desired product LXIa as a colorless liquid. Yield: 0.7 g, (74.0%); LCMS Calculated. for C 23 H 27 N 3 O 5 is 425.19; Observed: 426.35 [M+H] + .

Step-2: tert-butyl (3-(3-(2-aminoethyl)-2-oxopyridin-1(2H)-yl) propyl) carbamate (LXI)

To a solution of tert-butyl (3-(3-(2-(1,3-dioxoisoindolin-2-yl) ethyl)-2-oxopyridin-1(2H)-yl) propyl) carbamate LXIa (0.7 g, 2 mmol) in methanol (10 mL) at rt was added hydrazine hydrate (0.2 mL, 3 mmol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion, the reaction mixture was concentrated, added water (2 mL) and acidified with conc. HCl to pH-2. The solids were filtered, basified by 2N sodium hydroxide, and extracted with dichloromethane (200 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the desired product as a colorless viscous liquid. The crude compound LXI was taken for the next step without any further purification. Yield: 0.38 g, (80%); LCMS Calculated. for C 15 H 25 N 3 O 3 is 295.18; Observed: 296.35 [M+H] + .

Synthesis of 3-(2-aminoethyl)-1-(2-((tert-butyl diphenyl silyl) oxy) ethyl) pyridine-2(1H)-one. (LXII)

Step-1: tert-butyl (3-(3-(2-(1,3-dioxoisoindolin-2-yl) ethyl)-2-oxopyridin-1(2H)-yl) propyl) carbamate (LXIIa)

To a solution of 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (1.2 g, 4.5 mmol) in DMF (10 mL) were added (2-bromoethoxy) (tert-butyl) diphenyl silane (CAS: 139897-19-1, 2.0 g, 5.4 mmol) and cesium carbonate (2.2 g, 6.7 mmol). The reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, added water (50 mL) and extracted with ethyl acetate (200 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude compound was purified by silica gel (60-120 mesh) column chromatography with ethyl acetate in hexane (0-50%) to afford the desired product LXIIa as a pale-yellow solid. Yield:1.2 g, (48%); LCMS Calculated. for C 33 H 34 N 2 O 4 Si is 550.22; Observed: 551.40 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.79 (q, J=2.8 Hz, 2H), 7.68-7.67 (m, 2H), 7.53 (d, J=7.2 Hz, 4H), 7.42-7.26 (m, 7H), 7.14 (d, J=6.4 Hz, 1H), 5.97 (t, J=7.2 Hz, 1H), 4.08-3.93 (m, 6H), 2.90 (t, J=8.0 Hz, 2H), 1.01 (s, 9H).

Step-2: tert-butyl (3-(3-(2-aminoethyl)-2-oxopyridin-1(2H)-yl) propyl) carbamate (LXII)

To a solution of 2-(2-(1-(2-((tert-butyl diphenyl silyl) oxy) ethyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione LXIIa (1.2 g, 2.2 mmol) in methanol (4 mL) was added hydrazine hydrate (0.13 g, 2.6 mmol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion, the reaction mixture was concentrated, added water (2 mL) and acidified with conc. HCl to pH˜2. The solids were filtered, basified by 2N NaOH and extracted with 10% methanol in dichloromethane (200 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated to afford the desired product LXII as a pale-yellow viscous liquid. The crude compound was taken for the next step without any further purification. Yield: 7 g; LCMS Calculated. for C 2 H 32 N 2 O 2 Si is 420.22; Observed: 421.35 [M+H] + .

›Abbreviations · 40 of 69

Synthesis of 2-(6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl) pyridin-2-yl) ethan-1-amine. (LXIII)

Step-1: Synthesis of methyl 6-(2,2,2-trifluoro-1-hydroxyethyl) picolinate (LXIIIa)

A stirred solution of methyl 6-formylpicolinate (CAS: 69950-65-8, 2.5 g, 15 mmol) in THF (10 mL) was cooled to 0° C. under inert atmosphere and were added CsF (4.6 g, 30 mmol) and trimethyl(trifluoromethyl)silane (CAS: 81290-20-2, 3.2 g, 23 mmol). The reaction mixture was stirred at rt for 4 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (30 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was concentrated under reduced pressure. The crude was triturated with n-pentane (20 mL×3) and dried to afford the desired compound LXIIIa as a pale-yellow solid. Yield: 3.0 g (83.0%); LCMS Calculated. for C 9 H 8 F 3 NO 3 is 235.05; Observed: 236.20 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 8.18 (d, J=7.6 Hz, 1H), 7.96 (t, J=7.6 Hz, 1H), 7.65 (d, J=8.0 Hz, 1H), 5.17-5.14 (q, J=6.4 Hz, 1H), 4.01 (s, 3H)

Step-2: methyl 6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl) picolinate (LXIIIb)

A stirred solution of methyl 6-(2,2,2-trifluoro-1-hydroxyethyl) picolinate LXIIIa (3 g, 0.01 mol) in DCM (500 mL) was cooled to 0° C. and added imidazole (2 g, 0.03 mol). This was followed by drop wise addition of tert-butyl choro diphenyl silane (7 g, 0.03 mol). The reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (50 mL) and the resulting mixture was extracted with ethyl acetate (200 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude was purified by silica gel (230-400) column chromatography using 10-20% ethyl acetate/n-hexane to afford the desired compound LXIIIb as a pale-yellow liquid. Yield: 6.0 g, (100%); LCMS Calculated. for C 25 H 26 F 3 NO 3 Si is 473.16; Observed: 474.25 [M+H] + .

Step-3: (6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl)pyridin-2-yl)methanol (LXIIIc)

A stirred solution of methyl 6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl) picolinate LXIIIb (6 g, 0.01 mol) in MeOH (60 mL) was cooled to 0° C. and NaBH 4 (2 g, 0.05 mol) was added in portions. The reaction mixture was stirred at rt for 16 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated and added sat. NaHCO 3 solution (500 mL). The resulting mixture was extracted with DCM (600 mL×3) and concentrated under reduced pressure. The crude was purified by silica gel (230-400 mesh) column chromatography using 10-50% ethyl acetate in n-hexane to afford the desired compound LXIIIc as a pale-yellow viscous liquid. Yield: 4.5 g (75%); LCMS Calculated. for C 24 H 26 F 3 NO 2 Si is 445.17; Observed. 446.30 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.64 (q, J=7.6 Hz, 3H), 7.51 (d, J=7.6 Hz, 1H), 7.44 (d, J=6.8 Hz, 3H), 7.38-7.32 (m, 3H), 7.26-7.20 (m, 2H), 7.11 (t, J=7.6 Hz, 1H), 5.07 (q, J=6.4 Hz, 1H), 4.61 (d, J=4.8 Hz, 2H), 3.41 (t, J=4.8 Hz, 1H), 1.55 (s, 1H), 1.08 (s, 9H).

Step-4: (6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl) pyridin-2-yl) methyl methane sulfonate (LXIIId)

A stirred solution of (6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl) pyridin-2-yl) methanol LXIIIc (4.5 g, 10 mmol) in DCM (100 mL) was cooled to 0° C. and added TEA (6.1 g, 8.4 mL, 61 mmol). The resulting mixture was stirred for 5 min and mesyl-Cl (1.2 mL, 15 mmol) was added dropwise. The reaction mixture was stirred at 0° C. for 30 min and the progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with sat. NaHCO 3 solution (100 mL) and extracted with DCM (300 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford desired compound LXIIId as a brown liquid. The crude compound was taken for next step without further purification. Yield: 5 g (96%).

Step-5: 2-(6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl) pyridin-2-yl) acetonitrile (LXIIIe)

To a stirred solution of (6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl) pyridin-2-yl) methyl methane sulfonate LXIIId (6 g, 11.4 mmol) in DMF (60 mL) was added NaCN (1.34 g, 22.9 mmol) and the reaction mixture was stirred at rt for 24 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (50 mL) and the resulting mixture was extracted with ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude was purified by silica gel (230-400 mesh) column chromatography using 10-30% ethyl acetate in n-hexane to afford the desired compound LXIIIe a pale-yellow viscous liquid. Yield: 2.5 g (83.0%); LCMS Calculated. for C 25 H 25 F 3 N 2 OSi is 454.17; Observed.: 455.30 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.72-7.67 (m, 2H), 7.59 (t, J=8.0 Hz, 2H), 7.46-7.32 (m, 6H), 7.25-7.21 (m, 2H), 5.06 (q, J=6.4 Hz, 1H), 3.73 (d, J=8.0 Hz, 2H) 1.07 (bs, 9H)

Step-6: 2-(6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl) pyridin-2-yl) ethan-1-amine (LXIII)

To a stirred solution of 2-(6-(1-((tert-butyl diphenyl silyl) oxy)-2,2,2-trifluoroethyl) pyridin-2-yl) acetonitrile LXIIIe (2.5 g, 5.5 mmol) in THF (40 mL) at 0° C. was dropwise added BH 3 ·DMS (11 mL, 2 M in THF, 22.0 mmol). The reaction mixture was stirred at 70° C. for 5 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. before it was quenched with MeOH (7 mL). The mixture was stirred for 30 min and added 1 M HCl solution (10 mL). The mixture was concentrated to half under reduced pressure and extracted with ethyl acetate (50 mL) followed by with DCM (50 mL) to get rid of impurities. The aqueous layer was basified (pH 8-9) with 2N NaOH and extracted with DCM (500 mL×3), dried over sodium sulphate, and concentrated under reduced pressure to afford the desire compound LXIII as a brown liquid. The crude compound taken for next step without further purification. Yield: 1.6 g (64%); LCMS Calculated. for C 25 H 29 F 3 N 2 OSi is 458.20; Observed.: 459.35 [M+H]

›Abbreviations · 41 of 69

Synthesis of 7-amino-3-(cyclo butyl methyl)-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile. (LXIV)

Step-1: 3-cyclobutylpropanenitrile (LXIVa)

In a 3 neck RB flask, acetonitrile (CAS:75-05-8, 1.4 g, 1.8 mL, 34 mmol) was taken in dry THF (74.2 mL) and the solution was cooled to −78° C. To this was dropwise added LDA (17 mL, 2.0 M in THF/heptane/ethylbenzene, 34 mmol) under inert atmosphere. The reaction mixture was stirred at −78° C. for 40 min and a solution of (bromomethyl)cyclo butane (CAS: 17247-58-4, 3.8 mL, 34 mmol) in dry THF (15 mL) was added drop wise at −78° C. The reaction mixture was stirred at room temperature for 16 h. The reaction mixture was cooled to 0° C. and quenched with saturated ammonium chloride solution (65 mL). The resulting mixture was extracted with DCM (50 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude was purified by flash column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound LXIVa as a pale-yellow liquid. Yield: 1.65 g, (37.2%).

Step-2: 2-(cyclo butyl methyl)-3-oxobutanenitrile (LXIVb)

In a 3 neck RB flask, LDA (5.0 mL, 2.0 M in THF/heptane/ethylbenzene, 10 mmol) was diluted with dry THF (11.5 mL) under inert atmosphere and cooled to −78° C. To this was dropwise added a solution 3-cyclobutylpropanenitrile LXIVa (1.1 g, 10 mmol) in dry THF (6.0 mL) at −78° C. The reaction mixture was stirred at −78° C. for 1 h. Then dry ethyl acetate (CAS: 141-78-6, 0.81 g, 0.90 mL, 9.2 mmol) was drop wise added at −78° C. and stirring was continued for additional 1 h maintaining reaction temperature at −78° C. The reaction mixture was quenched with saturated ammonium chloride solution (6.0 mL) and extracted with diethyl ether (20 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the desired product LXIVb as a brown liquid. The crude product was taken for the next step without further purification. Yield: (1.65 g, 37.24%).

Step-3: 4-(cyclobutylmethyl)-5-methyl-1H-pyrazol-3-amine (LXIVc)

To a solution of 2-(cyclobutylmethyl)-3-oxobutanenitrile LXIVb (1.5 g, 9.9 mmol) in ethanol (16 mL) was added hydrazine hydrate (4.5 mL, 92 mmol) and the reaction mixture was stirred at 120° C. for 1.5 h. The progress of reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to room temperature and concentrated under reduced pressure. The residue was washed with n-hexane (20 mL×2) and dried under vacuo. To the residue was added water (10 mL) and extracted with 40% isopropanol in chloroform (15 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the desired product LXIVc as a brown oil. The crude was taken for the next step without further purification. Yield: (0.711 g, 44.43%); LCMS Calculated for C 9 H 15 N 3 is 165.13; Observed:166.30 [M+H] + .

Step-4: 7-amino-3-(cyclobutylmethyl)-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (LXIVd)

To a solution of 4-(cyclobutylmethyl)-5-methyl-1H-pyrazol-3-amine (707 mg, 4.28 mmol) in pyridine (7 mL) was added 2-(bis (methylthio) methylene) malononitrile LXIVc (728 mg, 4.28 mmol) under inert atmosphere. The reaction mixture was stirred at 120° C. for 3 h and progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to room temperature and quenched with ice cold water. The mixture was extracted with ethyl acetate (10 mL×3). The combined organic layer was washed with brine, dried over anhydrous sodium sulphate, and concentrated under reduced pressure to get crude material. The crude material was purified by silica gel (100-200) column chromatography using 0-20% ethyl acetate in n-hexane to afford the desired LXIVd as an off-white solid. Yield: 0.40 g (32.5%); LCMS Calculated. for C 14 H 17 N 5 S is 287.12; Observed: 288.25 [M+H] + .

Step-5: 7-amino-3-(cyclobutylmethyl)-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyridine-6carbonotrile (LXIV)

A solution of 7-amino-3-(cyclobutylmethyl)-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile LXIVd (400 mg, 1.39 mmol) in DCM (10 mL) was cooled to 0° C. and m-CPBA (961 mg, 5.57 mmol) was added in portions. The reaction mixture was stirred at room temperature for 3 h. Progress of reaction was monitored by TLC analysis. After completion, the reaction mixture was quenched by slow addition of saturated sodium bicarbonate solution (50 mL). The resulting mixture was extracted with DCM (20 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to get crude material. The crude material was purified by flash column chromatography (combi-flash) using 0-30% ethyl acetate in n-hexane to afford the desired product LXIV as an off white-solid. Yield: (203 mg (45.61%); LCMS Calculated. for C 14 H 17 N 5 O 2 S is 319.11, Observed. 320.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 9.19 (bs, 2H), 3.42 (s, 3H), 2.73 (d, J=7.6 Hz, 2H), 2.61-2.58 (m, 1H), 2.41 (s, 3H), 1.93 (d, J=4.4 Hz, 1H), 1.80-1.67 (m, 4H).

Synthesis of 2-(6-(2-((tert-butyl dimethyl silyl) oxy) ethyl) pyridin-2-yl) ethan-1-amine. (LXV)

Step-1: ethyl 2-(6-bromopyridin-2-yl) acetate (LXVa)

A stirred solution of lithium diisopropylamide (110 mL, 2.0 M in THF/heptane/ethylbenzene, 0.22 mol) in THF (500 mL) was cooled to −78° C. and added 2-bromo-6-methylpyridine (CAS: 5315-25-3, 15 g, 87 mmol) under N 2 atmosphere. The mixture was stirred at −78° C. for 30 min followed by a dropwise addition of diethyl carbonate (CAS: 105-58-8, 26 g, 0.22 mol) and the reaction mixture was stirred at −40° C. for 4 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with saturated ammonium chloride solution (150 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was dried over anhydrous sodium sulfate and concentrated under reduced pressure. The crude residue was subjected to silica gel (230-400) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound LXVa as a colorless liquid. Yield: (12.2 g, 57%); LCMS Calculated. for C 9 H 10 BrNO 2 is 242.98; Observed. 244.15 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.53 (t, J=7.6 Hz, 1H), 7.39 (d, J=7.6 Hz, 1H), 7.30-7.27 (m, 1H), 4.18 (q, J=7.2 Hz, 2H), 3.83 (s, 2H), 1.27 (t, J=7.2 Hz, 3H).

›Abbreviations · 42 of 69

Step-2: 2-(6-bromopyridin-2-yl) ethan-1-ol. (LXVb)

To a stirred solution of ethyl 2-(6-methylpyrazin-2-yl) acetate LXVa (3 g, 10 mmol) in ethanol (30 mL) was added sodium borohydride (2.32 g, 61.47 mmol) at 0° C. in portions under N 2 atmosphere and the reaction mixture was stirred at room temperature for 12 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (50 mL) and the resulting mixture was extracted with ethyl acetate (30 mL×3). The combined organic layer was dried over sodium sulphate and concentrated under reduced pressure to afford the pure compound LXVb as colorless viscous liquid. Yield: (1.7 g, 70%); LCMS Calculated. for C 7 H 8 BrNO is 200.97; Observed. 202.00 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.48 (t, J=8.0 Hz, 1H), 7.35 (d, J=8.0 Hz, 1H), 7.14 (d, J=8.0 Hz, 1H), 4.02 (q, J=5.2 Hz, 2H), 3.03-3.00 (m, 3H).

Step-3: 2-bromo-6-(2-((tert-butyldimethylsilyl) oxy) ethyl) pyridine (LXVc)

To a stirred solution of 2-(6-bromopyridin-2-yl) ethan-1-ol LXVb (1.7 g, 8.4 mmol) in DCM (25 mL) was added 1H-imidazole (1.1 g, 17 mmol) and the reaction mixture stirred at room temperature for 30 min under nitrogen followed by drop wise addition of tert-butyl choro dimethyl silane (2.5 g, 17 mmol) at 0° C. The resulting mixture was stirred at room temperature for 16 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was quenched with water (50 mL) and extracted with DCM (20 mL×2). The combined organic layer was dried over anhydrous sodium sulphate. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using (0-6%) ethyl acetate in n-hexane to afford the desired compound LXVc as a colorless viscous liquid. Yield: 2.3 g (86%); LCMS Calculated. for C 3 H 22 BrNOSi is 315.06; Observed. 316.25 [M+H] + ; 1 HNMR (400 MHz, CDCl 3 ): δ 7.44 (t, J=7.6 Hz, 1H), 7.31 (d, J=7.6 Hz, 1H), 7.16 (d, J=7.6 Hz, 1H), 3.96 (t, J=6.0 Hz, 2H), 2.95 (t, J=6.0 Hz, 2H), 0.82 (s, 9H), 0.06 (s, 6H).

Step-4: 2-(2-((tert-butyldimethylsilyl) oxy) ethyl)-6-vinylpyridine (LXVd)

To a stirred solution of 2-bromo-6-(2-((tert-butyldimethylsilyl) oxy) ethyl) pyridine LXVc (2.3 g, 7.3 mmol) was added potassium trifluoro(vinyl)borate (1.1 g, 8.0 mmol) and potassium phosphate tribasic (2.3 g, 11 mmol) in 1,4 dioxane (30 mL) and purged with N 2 gas for 15 min with vigorous stirring. To the reaction was added PdCl 2 (dppf) (0.27 g, 0.36 mmol) and the seal tube with Teflon screw-stopper was closed and heated to 110° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to room temperature and concentrated under reduced pressure. To the residue was added 70 mL of water and extracted with ethyl acetate (50 mL×2). The combined organic layer was dried over anhydrous sodium sulphate and concentrated to obtain crude product. The crude product was subjected to silica gel (230-400 mesh) column chromatography using 0-6% ethyl acetate in n-hexane to afford the desired compound LXVd as a colorless viscous liquid. Yield: 1.6 g (84%); LCMS Calculated. for C 15 H 25 NOSi is 263.17; Observed. 264.30 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.54 (t, J=7.6 Hz, 1H), 7.18 (d, J=7.6 Hz, 1H), 7.07 (d, J=7.6 Hz, 1H), 6.81 (dd, J=17.6, 10.8 Hz, 1H), 6.17 (d, J=17.6 Hz, 1H), 5.44 (d, J=10.8 Hz, 1H), 4.00 (t, J=6.4 Hz, 2H), 2.99 (t, J=6.4 Hz, 2H), 0.83 (s, 9H), 0.05 (s, 6H).

Step-5: 6-(2-((tert-butyldimethylsilyl) oxy) ethyl) picolinaldehyde (LXVe)

To a stirred solution of 2-(2-((tert-butyldimethylsilyl) oxy) ethyl)-6-vinylpyridine LXVd (1.6 g, 6.1 mmol) in THF (1.1 mL) and water (2.5 mL) was added osmium (VIII) oxide (3.8 mL, 4% in H 2 O, 0.61 mmol) at 0° C. and the reaction mixture was stirred at room temperature for 30 min. This was followed by portion wise addition of sodium metaperiodate (1.9 g, 9.1 mmol) and the reaction was stirred at room temperature for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (15 mL) and the resulting mixture was extracted with ethyl acetate (20 mL×2). The combined organic layer was dried over anhydrous sodium sulphate. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using 0-6% ethyl acetate in n-hexane to afford the desired compound LXVe as a colorless viscous liquid. Yield: 0.7 g (40%); LCMS Calculated. for C 14 H 23 NO 2 Si is 265.14; Observed. 266.30 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 10.06 (s, 1H), 7.82-7.75 (m, 2H), 7.44 (d, J=7.2 Hz, 1H), 4.03 (t, J=6.4 Hz, 2H), 3.09 (t, J=6.4 Hz, 2H), 0.82 (s, 9H), 0.06 (s, 6H).

Step-6: (E)-2-(2-((tert-butyldimethylsilyl) oxy) ethyl)-6-(2-nitrovinyl) pyridine (LXVf)

To a stirred solution of 6-(2-((tert-butyldimethylsilyl) oxy) ethyl) picolinaldehyde LXVe (0.7 g, 3 mmol) in DCM (20 mL) was added triethylamine (2.78 mL, 20 mmol) and nitromethane (0.3 mL, 8 mmol) under nitrogen atmosphere. The resulting mixture was stirred at room temperature for 2 h and the progress of the reaction was monitored by TLC analysis. After completion of reaction, DCM was concentrated under reduced pressure. The resulting residue was taken in DCM (20 mL) was added triethylamine (2.78 mL, 20 mmol) and cooled to 0° C. followed by drop wise addition of methane sulfonyl chloride (0.6 mL, 8 mmol). The resulting mixture was stirred at 0° C. for 30 min and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (40 mL) and extracted with DCM (20 mL×2). The combined organic layer was dried over anhydrous sodium sulphate. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using 0-6% ethyl acetate in n-hexane to afford the desired compound LXVf as a yellow viscous liquid. Yield: 0.7 g, (90%); LCMS Calculated. for C 15 H 24 N 2 O 3 Si is 308.15; Observed. 309.30 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 8.02 (d, J=13.2 Hz, 1H), 7.89 (d, J=13.2 Hz, 1H), 7.66 (t, J=7.6 Hz, 1H), 7.31-7.29 (m, 2H), 4.00 (t, J=6.4 Hz, 2H), 3.07-2.99 (m, 2H), 0.80 (s, 9H), 0.06 (s, 6H).

›Abbreviations · 43 of 69

Step-7: 2-(6-(2-((tert-butyldimethylsilyl) oxy) ethyl) pyridin-2-yl) ethan-1-amine (LXV)

To a stirred solution of aluminum (III) lithium hydride (10 mL, 1.0 M in THF, 10 mmol) in diethyl ether (70 mL) was dropwise added (E)-2-(2-((tert-butyldimethylsilyl) oxy) ethyl)-6-(2-nitrovinyl) pyridine LXVf (0.7 g, 3 mmol) at 0° C. under nitrogen atmosphere. The reaction mixture was stirred at room temperature for 2 h and the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. was added 1 mL of ice-cold water and 1 mL of 15% KOH solution then stirred at room temperature for another 30 min. The resulting reaction mixture was diluted with 250 mL of ethyl acetate filtered through Buchner funnel and the solid was thoroughly washed with 250 mL of ethyl acetate. The filtrate was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford a desired compound LXV as light brown viscous liquid. Yield: 0.54 g (85%); LCMS Calculated. for C 15 H 28 N 2 OSi is 280.20; Observed. 281.35 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 7.50 (t, J=7.6 Hz, 1H), 7.31-7.29 (m, 1H), 7.05-6.98 (m, 2H), 6.89 (d, J=8.4 Hz, 1H), 3.96 (t, J=6.4 Hz, 2H), 3.10-2.88 (m, 6H), 0.80 (s, 9H), 0.06 (s, 6H).

Synthesis of 2-(6-methylpyrazin-2-yl) ethan-1-amine (LXVI)

Step-1: Synthesis of ethyl 2-(6-methylpyrazin-2-yl) acetate (LXVIa)

To a stirred solution of 1.0 M lithium diisopropylamide (7 g, 0.07 L, 70 mmol) in THF (80 mL) was cooled to −78° C. was added 2,6-dimethylpyrazine (CAS:108-50-9, 3 g, 30 mol) under N 2 atmosphere and stirred at −78° C. for 30 min followed by dropwise addition of diethyl carbonate (CAS: 105-58-8, 4 g, 4 mL, 30 mol) and then the reaction mixture was stirred at −40° C. for 4 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with saturated ammonium chloride solution (50 mL) and extracted with ethyl acetate (30 mL×3). The combined organic layer was dried over anhydrous sodium sulfate and concentrated under reduced pressure. The crude residue was subjected to silica gel (230-400) column chromatography using 0-30% ethyl acetate in n-hexane to afford the desired compound LXVIa as a colorless liquid. Yield: 4.5 g (90%); LCMS Calculated. for C 9 H 12 N 2 O 2 is 180.08; Observed. 181.30 [M+H] + ; 1 HNMR (400 MHz, CDCl 3 ): δ 8.39-8.36 (m, 2H), 4.20 (q, J=7.2 Hz, 2H), 3.83 (s, 2H), 2.55 (s, 3H), 1.27 (t, J=7.2 Hz, 3H).

Step-2: 2-(6-methylpyrazin-2-yl) ethan-1-ol (LXVIb)

To a stirred solution of ethyl 2-(6-methylpyrazin-2-yl) acetate LXVIa (4.5 g, 25 mmol) in ethanol (100 mL) was added sodium borohydride (3.8 g, 100 mmol) at 0° C. in portions under N 2 atmosphere and the reaction mixture was stirred at room temperature for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (50 mL) and the resulting mixture was extracted with ethyl acetate (30 mL×3). The combined organic layer was dried over sodium sulphate and concentrated under reduced pressure to afford the pure compound LXVIb as colorless viscous liquid. Yield: (3.5 g, 100%); LCMS Calculated. for C 7 H 10 N 2 O is 138.07; Observed. 139.30 [M+H] + ; 1 HNMR (400 MHz, CDCl 3 ): δ 8.33-8.29 (m, 2H), 5.31 (t, 1H), 4.03 (t, J=5.6 Hz, 2H), 3.02 (t, J=5.6 Hz, 2H) 2.55 (s, 3H).

Step-3: 2-(6-methylpyrazin-2-yl) ethyl methane sulfonate (LXVIc)

To a stirred solution of 2-(6-methylpyrazin-2-yl) ethan-1-ol LXVIb (3.5 g, 25 mmol) in DCM (100 mL) was added triethylamine (6.4 g, 8.8 mL, 63 mmol) followed by dropwise addition of mesyl chloride (3.8 g, 2.6 mL, 33 mmol) at 0° C. and stirred the reaction mixture at 0° C. for 30 min. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with water (20 mL) and the resulting mixture was extracted with DCM (20 mL×3). The combined organic layer was dried over sodium sulphate and concentrated under reduced pressure to afford the pure compound LXVIc as a brown liquid. Yield: 5.0 g (90%); LCMS Calculated. for C 8 H 12 N 2 O 3 S is 216.05; Observed. 217.25 [M+H] + ; 1 HNMR (400 MHz, CDCl 3 ): δ 8.35-8.31 (m, 2H), 4.65 (t, J=6.0 Hz, 2H), 3.21 (t, J=6.4 Hz, 2H), 3.01 (s, 3H) 2.55 (s, 3H).

Step-4: 2-(2-azidoethyl)-6-methylpyrazine (LXVId)

To a solution of 2-(6-methylpyrazin-2-yl) ethyl methane sulfonate LXVIc (5 g, 20 mmol) in DMF (50 mL) was added sodium azide (6 g, 90 mmol) and the resulting mixture was stirred at 80° C. for 4 h under N 2 atmosphere. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was quenched with ice cold water (20 mL) and the resulting mixture was extracted with ethyl acetate (20 mL×3). The combined organic layer was dried over sodium sulphate and concentrated under reduced pressure to afford the pure compound LXVId as a brown viscous liquid. Yield: 3.9 g (97.5%); LCMS Calculated. for C 7 H 9 N 5 is 163.08; Observed. 164.05 [M+H] + ; 1 HNMR (400 MHz, CDCl 3 ): δ 8.38-8.31 (m, 2H), 3.73 (t, J=6.4 Hz, 2H), 3.04 (t, J=6.8 Hz, 2H), 2.56 (s, 3H).

Step-5: 2-(6-methylpyrazin-2-yl) ethan-1-amine (LXVI)

To a stirred solution of 2-(2-azidoethyl)-6-methylpyrazine LXVId (3.9 g, 24 mmol) in methanol (80 mL) was added Pd/C (10%, 1.3 g, 12 mmol) under N 2 atmosphere and stirred at room temperature for 48 h under H 2 bladder. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was filtered through celite bed, washed with methanol (25 ml×2) and concentrated under reduced pressure to afford the desired compound LXVI as a colorless viscous liquid. Yield: 2.2 g (67%); LCMS Calculated. for C 7 H 11 N 3 is 137.09; Observed. 138.30 [M+H] + ; 1 HNMR (400 MHz, CDCl 3 ): δ 8.29 (d, J=8.8 Hz, 2H), 3.10 (t, J=6.8 Hz, 2H), 2.96-2.89 (m, 4H), 2.55 (s, 3H).

Synthesis of 3-(2-aminoethyl)-1-(2-(methylthio) ethyl) pyridine-2(1H)-one (LXVII)

Step-1: 2-(2-(1-(2-(methylthio) ethyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione (LXVIIa)

To a stirred solution of 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (8 g, 0.03 mol) in DMF (80 mL) was added Cs 2 CO 3 (10 g, 0.04 mol) at rt under N 2 atmosphere. This was followed by a drop wise addition of (2-chloroethyl) (methyl) sulfane (CAS: 54187-93-8, 4 g, 0.04 mol) and the reaction mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis which indicated the formation of less polar spot corresponds to O-alkylated product and polar spot corresponds to N-alkylated product. After completion of the reaction, water (100 ml) was added, and the mixture was extracted with ethyl acetate (150 mL×2). The combined organic layer was washed with brine, dried over anhydrous sodium sulfate, and concentrated over reduced pressure. The crude compound was purified by flash column chromatography (silica gel, 230-400) using ethyl acetate in n-hexane (0-40%) to afford the desired compound LXVIIa as a colorless viscous liquid. Yield: 6.0 g (60%); LCMS Calculated. for C 18 H 18 N 2 O 3 S is 342.10; Observed. 343.25. [M+H] + . 1 H NMR (400 MHz, CDCl 3 ) δ 7.80 (q, J=2.8 Hz, 2H), 7.69 (q, J=3.2 Hz, 2H), 7.22 (t, J=6.4 Hz, 1H), 7.12 (d, J=6.0 Hz, 1H), 6.02 (t, J=6.4 Hz, 1H), 4.10-4.00 (m, 4H), 3.31-2.70 (m, 4H), 2.07 (s, 3H).

›Abbreviations · 44 of 69

Step-2: 3-(2-aminoethyl)-1-(2-(methylthio) ethyl) pyridin-2(1H)-one (LXVII)

To a stirred solution of 2-(2-(1-(2-(methylthio) ethyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione LXVIIa (6.0 g, 17.52 mmol) in MeOH (60 mL) was added hydrazine hydrate (1.316 g, 26.28 mmol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated, and the residue was dissolved in water (10 mL). The resulting mixture was acidified (pH=2) with conc. HCl and the solid formed was removed by filtration. The filtrate was basified with 4N NaOH solution (pH=8-9) and extracted with methanol: DCM (0.5:9.5 v/v) (300 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the desired compound LXVII as a sticky solid. The crude compound was taken for next step without further purification. Yield: 4.0 g (100%); LCMS Calculated. for C 10 H 16 N 2 OS is 212.10; Observed. 213.25 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 8.02 (s, 4H), 7.24 (t, J=13.6 Hz, 2H), 6.14 (t, J=6.8 Hz, 1H), 4.11 (d, J=6.8 Hz, 2H), 3.47 (s, 2H), 2.94 (m, 2H), 2.68 (t, J=6.8 Hz, 2H), 2.11 (s, 3H).

Synthesis of N-(3-(3-(2-aminoethyl)-2-oxopyridin-1(2H)-yl) propyl) acetamide (LXVIII)

Step-1: 2-(2-(1-(3-aminopropyl)-2-oxo-1,2-dihydropyridin-3-yl)ethyl)isoindoline-1,3-dione (LXVIIIa)

To a stirred solution of tert-butyl (3-(3-(2-(1,3-dioxoisoindolin-2-yl)ethyl)-2-oxopyridin-1(2H)-yl)propyl)carbamate LXIa (700 mg, 1.65 mmol) in 1,4-dioxane (4 mL) was added HCl in dioxane (0.412 mL, 4 molar, 1.65 mmol) at 0° C. under N 2 atmosphere. The reaction mixture was stirred at rt for 2 h and progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under reduced pressure, the residue was triturated with diethyl ether and dried under vacuo to afford the desired compound LXVIIIa as an off-white solid. Yield: 430 mg (80%); LCMS Calculated. for C 18 H 19 N 3 O 3 is 325.14; Observed.: 326.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): 8.06 (s, 3H), 7.83 (d, J=1.6 Hz, 4H), 7.63 (d, J=5.2 Hz, 1H), 7.19 (d, J=5.6 Hz, 1H), 6.10 (t, J=6.4 Hz, 1H), 3.98 (t, J=6.4 Hz, 2H), 3.85 (t, J=6.0 Hz, 2H), 2.79-2.72 (m, 3H), 1.99-1.92 (m, 2H).

Step-2: N-(3-(3-(2-(1,3-dioxoisoindolin-2-yl) ethyl)-2-oxopyridin-1(2H)-yl) propyl) acetamide (LXVIIIb)

To a stirred solution of 2-(2-(1-(3-aminopropyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione LXVIIIa (400 mg, 1.23 mmol) in THF (10 mL) were added TEA (171 μL, 1.23 mmol) and DMAP (15.0 mg, 123 μmol) at rt under N 2 atmosphere. This was followed by a drop wise addition of acetic anhydride (139 μL, 1.48 mmol) and the resulting reaction mixture was stirred at rt for 3 h. The progress of the reaction was monitored by TLC analysis. After completion, sat. NaHCO 3 solution (10 mL) was added, and the resulting mixture was extracted with DCM (50 ml×2). The combined organic layer was dried over anhydrous sodium sulfate and concentrated under reduced pressure to afford the desired compound LXVIIIb as an off-white solid. Yield: 350 mg (78%); LCMS Calculated. for C 20 H 21 N 3 O 4 is 367.15; Observed.: 368.30 [M+H] + .

Step-3: N-(3-(3-(2-aminoethyl)-2-oxopyridin-1(2H)-yl) propyl) acetamide (LXVIII)

To a stirred solution of N-(3-(3-(2-(1,3-dioxoisoindolin-2-yl) ethyl)-2-oxopyridin-1(2H)-yl) propyl) acetamide LXVIIIb (350 mg, 953 μmol) in MeOH (5 mL) was added hydrazine hydrate (71.5 mg, 1.43 mmol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated, and the residue was dissolve in water (2 mL). The mixture was acidified (pH=2) using conc. HCl, the solid formed was filtered and washed with water. The filtrate was basified with 4N NaOH solution (pH=8-9) and extracted with DCM (50 ml×3). The combined organic layer was dried over anhydrous sodium sulfate and concentrated over reduced pressure. The crude compound LXVIII was taken for next step without further purification. Yield: 210 mg (93%); LCMS Calculated. for C 12 H 19 N 3 O 2 is 237.14; Observed. 238.30 [M+H] + .

Synthesis of 2-(6-(2-methoxyethoxy) pyridin-2-yl) ethan-1-amine (LXIX)

Step-1: (E)-2-methoxy-6-(2-nitrovinyl) pyridine (LXIXa)

To a stirred solution of 6-methoxypicolinaldehyde (CAS:54221-96-4, 3 g, 0.02 mol) in DCM (30 mL) under inert atmosphere were added TEA (6 mL, 0.04 mol) and nitromethane (2 g, 0.03 mol) at room temperature. The reaction mixture was stirred at rt for 2 h and the progress of the reaction was monitored by TLC analysis. The reaction mixture was concentrated to dryness under reduced pressure and the residue was dissolved in fresh DCM (40 mL). The solution was cooled to 0° C. under inert atmosphere and was added TEA (9 mL, 0.07 mol). This was followed by a dropwise addition of mesyl-Cl (3 mL, 0.03 mol) over 10 min. The resultant mixture was stirred at 0° C. for 30 min and quenched with saturated solution of NaHCO 3 . The mixture was extracted with DCM (100 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated over reduced pressure. The crude was purified by silica-gel (230-400) column chromatography using 10-30% ethyl acetate in n-hexane to afford the desired compound LXIXa as a pale-yellow solid. Yield: 1.8 g (45%). LCMS Calculated. for C 8 H 8 N 2 O 3 is 180.05; Observed. 181.25; [M+H] + : 1 H NMR (400 MHz, CDCl 3 ): δ 8.00 (d, J=12.8 Hz, 1H), 7.83 (d, J=12.8 Hz, 1H), 7.64 (t, J=7.2 Hz, 1H), 7.06 (d, J=7.2 Hz, 1H), 6.85 (d, J=8.4 Hz, 1H), 3.97 (s, 3H).

Step-2: 2-(6-methoxypyridin-2-yl) ethan-1-amine (LXIXb)

A stirred solution of LiAlH 4 (120 mL, 1 M in THF, 0.12 mol) in diethyl ether (300 mL) was cooled to 0° C. under inert atmosphere and dropwise added a solution of (E)-2-methoxy-6-(2-nitrovinyl) pyridine LXIXa (7.3 g, 41 mmol) in THF (30 mL) over 15 min. The resulting mixture was stirred at room temperature for 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to 0° C. and quenched with ice cold water (24 mL). Then added 15% NaOH solution (8 ml) and ethyl acetate (200 mL). The mixture was stirred at rt for 15 min and filtered. The residue was washed thoroughly with ethyl acetate (500 mL×3). The combined filtrate was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford the desired compound LXIXb as a pale-yellow viscous liquid. Yield: 5.0 g (80.6%). LCMS Calculated. for C 8 H 2 N 2 O is 152.09; Observed. 153.30; [M+H] + .

›Abbreviations · 45 of 69

Step-3: 2-(2-(6-methoxypyridin-2-yl) ethyl) isoindoline-1,3-dione (LXIXc)

To a stirred solution of 2-(6-methoxypyridin-2-yl) ethan-1-amine LXIXb (5 g, 0.03 mol) in acetic acid (50 mL) was added in isobenzofuran-1,3-dione (6 g, 0.04 mol) under inert atmosphere. The resulting mixture was stirred at 140° C. for 3 h. Then reaction mixture was concentrated under reduced pressure to dryness. To the residue was added acetic anhydride (20 mL, 0.2 mol) and stirring at 140° C. was continued for additional 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to rt and quenched with sat. NaHCO 3 solution (200 mL). The resulting mixture was extracted with DCM (300 mL×3) and the combined organic layer was dried over anhydrous sodium sulphate. The organic layer was filtered and concentrated under reduced pressure. The crude was purified by silica-gel (230-400) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound LXIXc as off-white solid. Yield: 5.0 g (55%). LCMS Calculated. for C 16 H 14 N 2 O 3 is 282.10; Obs. 283.20; [M+H] + .

Step-4: 2-(2-(6-oxo-1,6-dihydropyridin-2-yl) ethyl) isoindoline-1,3-dione (LXIXd)

To a stirred solution of 2-(2-(6-methoxypyridin-2-yl) ethyl) isoindoline-1,3-dione LXIXc (5.0 g, 18 mmol) in CHCl 3 (80 mL) was added TMS-I (4.8 mL, 35 mmol) and the reaction mixture was stirred at 85° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, reaction was cooled to rt and quenched with MeOH (20 mL). The resulting mixture was concentrated under reduced pressure and the residue was triturated in MTBE/EtOH (5:1). The solid formed was collected by filtration and dried to afford the desired compound LXIXd as an off-white solid. Yield: 2.8 g (58%). LCMS Calculated. for C 15 H 12 N 2 O 3 is 268.09; Observed. 269.25; [M+H] + ; 1 H NMR (400 MHz, CD3OD): δ 7.84 (bs, 4H), 7.54 (t, J=7.2 Hz, 1H), 6.44 (d, J=8.8 Hz, 1H), 6.33 (d, J=6.8 Hz, 1H), 3.90 (bs, 2H), 2.88 (bs, 2H).

Step-5: 2-(2-(6-(2-methoxyethoxy) pyridin-2-yl) ethyl) isoindoline-1,3-dione (LXIXe)

To a stirred solution of 2-(2-(6-oxo-1,6-dihydropyridin-2-yl) ethyl) isoindoline-1,3-dione LXIXd (0.70 g, 2.61 mmol) in DMF (10 mL) was added Cs 2 CO 3 (1.70 g, 5.22 mmol) and stirred for 20 min at rt. The mixture was cooled to 0° C. and added 1-chloro-2-methoxyethane (370 mg, 3.91 mmol) slowly under nitrogen atmosphere. The reaction was stirred at rt for 16 h and the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (50 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude was purified by combi flash column silica gel (230-400) column chromatography using 0-20% ethyl acetate in n-hexane to afford the desired compound LXIXe as a pale-yellow viscous liquid. The crude compound was taken for the next step without further purification; Yield: 0.35 g (39%).

Step-6: 2-(6-(2-methoxyethoxy) pyridin-2-yl) ethan-1-amine (LXIX)

To a stirred solution of 2-(2-(6-(2-methoxyethoxy)-1,6-dihydropyridin-2-yl) ethyl) isoindoline-1,3-dione LXIXe (0.65 g, 2.0 mmol) in methanol (10 mL) was added hydrazine hydrate (0.14 mL, 3.0 mmol) and the reaction was stirred for at rt for 3 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated, and the crude was diluted with 10% NaOH solution (50 mL). The mixture was extracted with ethyl acetate (20 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure to afford the desired compound LXIX as a pale-yellow viscous liquid. The crude taken for the next step without further purification. Yield: 0.5 g (80%)

Synthesis of 3-(2-aminoethyl)-1-(3-(methylthio) propyl) pyridin-2(1H)-one. (LXX)

Step-1: 2-(2-(1-(3-(methylthio) propyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione (LXXa)

To a solution of 2-(2-(2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione XLd (5.0 g, 18.64 mmol) in N, N-dimethyl formamide (15 mL) were added cesium carbonate (12.15 g, 37.28 mmol) and 3-(methylthio) propyl 4-methylbenzenesulfonate (CAS:187722-18-5, 5.82 g, 22.37 mmol). The reaction mixture was stirred at rt for 24 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, added water (300 mL) and extracted with ethyl acetate (500 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under reduced pressure. The crude compound was purified by silica gel (230-400 mesh) column chromatography with ethyl acetate in n-hexane (0-50%) to afford the desired product LXXa as a pale-yellow viscous liquid. Yield: 3.9 g, (59.0%); LCMS Calculated. for C 19 H 20 N 2 O 3 S is 356.12; Observed 357 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 8.02 (s, 3H), 7.80 (q, J=2.4 Hz, 2H), 7.68 (q, J=2.4 Hz, 2H), 4.02 (q, J=8 Hz, 4H), 2.91 (d, J=4 Hz, 4H), 2.52 (t, J=8 Hz, 2H), 2.11 (s, 1H), 2.04 (t, J=8 Hz, 2H).

Step-2: 3-(2-aminoethyl)-1-(3-(methylthio) propyl) pyridin-2(1H)-one (LXX)

To a solution of 2-(2-(1-(3-(methylthio) propyl)-2-oxo-1,2-dihydropyridin-3-yl) ethyl) isoindoline-1,3-dione LXXa (1.90 g, 5.330 mmol) in methanol (4 mL) was added hydrazine hydrate (0.32 g, 6.397 mmol) and the reaction mixture was stirred at rt for 2 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was concentrated, added water (5 mL) and acidified with conc. HCl to pH-1. The solids were filtered, basified by sodium hydroxide (1 N) and extracted with 10% methanol in dichloromethane (500 mL×3). The combined organic layer was dried over anhydrous sodium sulphate to afford the desired compound LXX as a pale-yellow viscous liquid. The crude compound was taken for the next step without any further purification. Yield: 0.9 g, (75.0%); LCMS Calculated. for C 11 H 18 N 2 OS is 226.11; Observed: 227.25 [M+H]+. 1 H NMR (400 MHz, CDCl 3 ): δ 7.22 (d, J=6.4 Hz, 2H), 6.13 (d, J=6.8 Hz, 1H), 4.05 (t, J=6.8 Hz, 2H), 2.95 (t, J=6.4 Hz, 3H), 2.68 (t, J=6.8 Hz, 2H), 2.53 (t, J=7.2 Hz, 2H), 2.11-2.01 (m, 6H).

›Abbreviations · 46 of 69

Synthesis of 3-(6-(2-aminoethyl) 222 yridine-2-yl) propan-1-ol (LXXI)

Step-1: 6-(3-((tert-butyldimethylsilyl) oxy) prop-1-yn-1-yl) picolinaldehyde (LXXIa)

To a stirred solution of 6-bromopicolinaldehyde (CAS: 34160-40-2, 1.0 g, 5.38 mmol) was added CuI (0.372 g, 1.61 mmol) and tetrakis(triphenyiphospiline)pailadium (0) (0.932 g, 0.81 mmol) in 2:1 THF/triethylamine (10 mL) and purged with N 2 gas for 15 min with vigorous stirring. To the reaction was added tert-butyl dimethyl(prop-2-yn-1-yloxy) silane (1.10 g, 6.45 mmol) and the seal tube with Teflon screw-stopper was closed and stirred at room temperature for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with 100 mL of water and extracted with ethyl acetate (50 mL×2). The combined organic layer was dried over anhydrous sodium sulphate and concentrated to obtain crude product. The crude product was subjected to silica gel (230-400 mesh) column chromatography using 0-6% ethyl acetate in n-hexane to afford the desired compound LXXIa as a colorless viscous liquid. Yield: (1.4 g, 95%); LCMS Calculate. for C 15 H 21 NO 2 Si is 275.13; Observed. 276.00 [M+H] + ; 1 HNMR (400 MHz, CDCl 3 ): δ 10.05 (s, 1H), 7.91 (dd, J=7.6, 1.2 Hz, 1H), 7.85 (t, J=7.6 Hz, 1H), 7.64 (dd, J=7.6, 1.2 Hz, 1H), 4.60 (s, 2H), 0.85 (s, 9H), 0.18 (s, 6H).

Step-2: (E)-2-(3-((tert-butyldimethylsilyl) oxy) prop-1-yn-1-yl)-6-(2-nitrovinyl) pyridine (LXXIb)

To a stirred solution of 6-(3-((tert-butyldimethylsilyl) oxy) prop-1-yn-1-yl) picolinaldehyde LXXIa (1.4 g, 5.1 mmol) in DCM (20 mL) was added triethylamine (1.5 g, 2.1 mL, 15 mmol) and nitromethane (4.7 g, 4.1 mL, 76 mmol) under nitrogen atmosphere. The resulting mixture was stirred at room temperature for 1 h and the progress of the reaction was monitored by TLC analysis. After completion of reaction, DCM was concentrated under reduced pressure. The resulting residue was taken in DCM (20 mL) was added triethylamine (1.5 g, 2.1 mL, 15 mmol) and cooled to 0° C. followed by drop wise addition of methane sulfonyl chloride (1.7 g, 1.2 mL, 7.6 mmol). The mixture was stirred at 0° C. for 30 min and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (40 mL) and extracted with DCM (20 mL×2). The combined organic layer was dried over anhydrous sodium sulphate. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using 0-6% ethyl acetate in n-hexane to afford the desired compound LXXIb as a yellow viscous liquid. Yield: 1.1 g (68%); LCMS Calculate. for C 16 H 22 N 2 O 3 Si is 318.14; Observed. 319.30 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ): δ 8.03 (d, J=13.2 Hz, 1H), 7.87 (d, J=13.2 Hz, 1H), 7.75 (t, J=8.0 Hz, 1H), 7.48 (d, J=8.0 Hz, 1H), 7.40 (d, J=8.0 Hz, 1H), 4.49 (s, 2H), 0.94 (s, 9H), 0.18 (s, 6H).

Step-3: 3-(6-(2-aminoethyl) pyridin-2-yl) propan-1-ol. (LXXI)

To a stirred solution of lithium aluminum hydride (13.8 mL, 1.0 M in THF, 14 mmol) in diethyl ether (70 mL) was added (E)-2-(2-((tert-butyldimethylsilyl) oxy) ethyl)-6-(2-nitrovinyl) pyridine LXXIb (1.1 g, 3.5 mmol) dropwise at 0° C. under nitrogen atmosphere. The reaction mixture was stirred at room temperature for 4 h and the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to 0° C. was added 1 mL of ice-cold water and 1 mL of 15% KOH solution then stirred at room temperature for another 30 min. The resulting reaction mixture was diluted with 100 mL of ethyl acetate filtered through Buchner funnel and the solid was thoroughly washed with 100 mL of ethyl acetate. The filtrate was dried over anhydrous sodium sulphate and concentrated under reduced pressure to afford a desired compound LXXI as light brown viscous liquid. Yield: (0.7 g, 85%).

Synthesis of 7-amino-2-methyl-5-(methyl sulfonyl)-3-propylpyrazolo[1,5-a] pyrimidine-6-carbonitrile. (LXXII)

Step-1: 2-acetylpentanenitrile (LXXIIa)

A stirred solution of lithium diisopropylamide (30 mL, 2.0 M in THF/heptane/ethylbenzene, 0.06 mol) in dry THF (300 mL) was cooled to −78° C. under inert atmosphere and dropwise added a solution of pentane nitrile (CAS:110-59-8, 5 g, 6.48 mL, 1 Eq, 0.06 mol) in THF (20 mL). The resulting mixture was stirred −78° C. for 1 h. To the reaction was dropwise added dry ethyl acetate (6 mL, 0.06 mol) over 10 min and the mixture was stirred at −78° C. for additional 2 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was quenched with saturated solution of NH 4 Cl (100 mL) and extracted with ethyl acetate (200 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure to afford the desired compound LXXIIa as a pale-yellow viscous liquid. Yield: 10.0 g.

Step-2: 5-methyl-4-propyl-1H-pyrazol-3-amine (LXXIIb)

To a stirred solution of 2-acetylpentanenitrile LXXIIa (10 g, 80 mmol) in ethanol (100 mL) was added hydrazine hydrate (5.8 mL, 0.12 mol) and the reaction mixture was stirred under inert atmosphere at 120° C. for 1.5 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and concentrated under reduced pressure. The residue obtained was diluted with water (100 mL) and extracted with 10% MeOH in DCM (200 mL×5). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure to afford the desired compound LXXIIb as a pale-yellow viscous liquid. The crude material was used in the next step without further purification. Yield: 9.3 g. (83.6%); LCMS Calculated. for C 7 H 13 N 3 is 139.11; Observed. 140.30 [M+H] + .

Step-3: 7-amino-2-methyl-5-(methylthio)-3-propylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (LXXIIc)

A mixture of 5-methyl-4-propyl-1H-pyrazol-3-amine (9.3 g, 67 mmol), 2-(bis(methylthio)methyl) malononitrile LXXIIb (13 g, 73 mmol) and pyridine (50 mL) was stirred at 100° C. under inert atmosphere for 3 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and poured into ice cold water (1000 mL). The precipitate formed was filtered and washed with cold water. The solid obtained was dried under vacuum to afford the title compound LXXIIc as a pale-yellow solid. Yield: 15 g (88%). LCMS Calculated. for C 12 H 15 N 5 S is 261.10; Observed. 262.20 [M+H] + .

›Abbreviations · 47 of 69

Step-4: 7-amino-2-methyl-5-(methylsulfonyl)-3-propylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (LXXII)

To a stirred solution of 7-amino-2-methyl-5-(methylthio)-3-propylpyrazolo [1,5-a] pyrimidine-6-carbonitrile LXXIIc (15 g, 57 mmol) in solvent DCM (1000 mL) at 0° C. was added mCPBA (50 g, 0.29 mol) in portions under inert atmosphere. The resulting mixture was stirred at room temperature for 18 h and progress of the reaction was monitored by TLC analysis. After completion, the solvent was removed under vacuum and to the yellow precipitate was added saturated sodium bicarbonate solution (250 mL). The resulting mixture was stirred at rt for 30 min, the solid was collected by filtration and dried under reduced pressure to afford the desired compound LXXII as a pale-yellow solid. Yield: 7.5 g (44%); LCMS: Calculated. for C 12 H 15 N 5 O 2 S is 293.09; Observed. 294.20 [M+H]

Synthesis of 2-(1-(3-(methylthio) propyl)-1H-pyrazol-3-yl) ethan-1-amine (LXXIII)

Step-1: 1-(3-(methylthio) propyl)-1H-pyrazole-3-carbaldehyde (LXXIIIa)

To a stirred solution of 1H-pyrazole-3-carbaldehyde (CAS:3920-50-1, 3 g, 0.0312 mol) in DMF (50 mL) was added Cs 2 CO 3 (15.27 g, 0.0469 mol) and the resulting reaction mixture was cooled to 0° C. To this was dropwise added of 3-(methylthio) propyl 4-methylbenzenesulfonate (CAS:187722-18-5, 9.75 g, 0.037 mol) under nitrogen. The resulting mixture was stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (2×200 mL). The combined organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure to afford the crude yellow liquid. The crude material was purified by column chromatography (silica 230-400) eluted with 0-10% ethyl acetate in n-hexane to afford the compound LXXIIIa as a pale-yellow liquid. Yield: 2.5 g (40%). 1 H NMR (400 MHz, DMSO-D 6 ): δ 9.87 (s, 1H), 7.96 (s, 1H), 6.78 (d, J=1.6 Hz, 1H), 4.33 (t, J=6.8 Hz, 2H), 2.44 (t, J=7.2 Hz, 2H), 2.11-2.08 (m, 2H), 2.05 (s, 3H).

Step-2: (E)-1-(3-(methylthio) propyl)-3-(2-nitrovinyl)-1H-pyrazole (LXXIIIb)

To a stirred solution of 1-(3-(methylthio) propyl)-1H-pyrazole-3-carbaldehyde LXXIIIa (1.1 g, 6.0 mmol) in toluene (20 mL) were added nitromethane (5.5 g, 90 mmol) and ammonium acetate (0.69 g, 9.0 mmol) under nitrogen atmosphere. The resulting mixture was stirred at 100° C. for 16 h and progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was cooled to room temperature, diluted with cold 1N HCl solution (15 mL) and extracted with ethyl acetate (100 mL×3). The combined organic layer was washed with water (100 mL). The organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure to afford the crude brown semi-solid. The crude material was purified by silica gel (100-200) column chromatography using 0-10% ethyl acetate in n-hexane to afford the desired compound LXXIIIb as a pale-yellow liquid. Yield: 0.9 g (66%); δ 7.96 (d, J=13.6 Hz, 1H), 7.61 (d, J=13.6 Hz, 1H), 7.47 (d, J=1.6 Hz, 1H), 6.53 (d, J=1.6 Hz, 1H), 4.30 (t, J=6.4 Hz, 2H), 2.46 (t, J=6.8 Hz, 2H), 2.22-2.15 (m, 2H), 2.10 (s, 3H).

Step-3: 2-(1-(3-(methylthio) propyl)-1H-pyrazol-3-yl) ethan-1-amine (LXXIII)

A stirred solution of LiAlH 4 (15.8 mL, 1 M in THF, 15.8 mmol) in diethyl ether (50 mL) was cooled to 0° C. and dropwise added solution of (E)-1-(3-(methylthio) propyl)-3-(2-nitrovinyl)-1H-pyrazole LXXIIIb (900 mg, 3.96 mmol) in diethyl ether (5 mL). The reaction mixture was stirred at rt for 2 h and progress of the reaction was monitored by TLC analysis. After completion, reaction mixture was cooled to 0° C. and quenched with water (0.9 mL), followed by with 15% KOH ((0.9 mL). Then water (1.8 mL) and ethyl acetate (50 mL) were added. The resulting reaction mixture was stirred at rt for 30 min. The mixture was filtered, and solid residue was washed with fresh ethyl acetate (250 mL×3). The combined filtrate was concentrated under reduced pressure to afford the desired compound LXXIII as a light brown viscous liquid. Yield: 700 mg (88.7%); LCMS Calculated. for C 9 H 17 N 3 S is 199.11; Observed. 200.25 [M+H] + .

Synthesis of (4-(3-(2-aminoethyl)-5-methyl-1H-pyrazol-1-yl) tetrahydrofuran-3-yl) methanol (LXXIV)

The title compound LXXIV was prepared in analogus to LVII by using 5-methyl-1H-pyrazole-3-carbaldehyde (CAS: 3273-44-7) instead of 1H-pyrazole-3-carbaldehyde (CAS: 3920-50-1). LXXIV was obtained as an off white solid. The crude product obtained is taken for the next step without further purification. LCMS Calculated. for C 11 H 19 N 3 O 2 is 225.29; Observed. 226.40 [M+H] + .

Example 1: 7-amino-2,3-dimethyl-5-{[1-(6-methylpyridin-2-yl) ethyl]amino}pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a mixture of 1 (80 mg, 0.30 mmol) and racemic 1-(6-methylpyridin-2-yl) ethan-1-amine (CAS: 58088-67-8) (65 mg, 0.45 mmol) in DMF (3 mL) was added Et 3 N (0.1 mL). The resulted mixture was irradiated under constant microwave for about 1 h at 120° C. After completion, the reaction mixture was concentrated in vacuo to get the crude. The crude was purified by reverse phase preparative HPLC to afford the title compound (Example 1) as white solid. Yield: 15 mg, 15.62%; LC-MS Calculated for C 17 H 19 N 7 : 321.39; Observed.: 322.1; [M + +H]. 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.21 (s, 2H), 7.69-7.64 (m, 1H), 7.27 (d, J=8.00 Hz, 1H), 7.14 (dd, J=1.60, 7.60 Hz, 1H), 6.97 (d, J=4.40 Hz, 1H), 5.28-5.25 (m, 1H), 2.50 (s, 3H) 2.22 (s, 3H), 1.95 (s, 3H), 1.51-1.48 (m, 3H). HPLC: 2.39 min; 98.85%; HPLC Column: Atlantis dC18 (250*4.6) mm Mobile Phase A: 0.1% TFA in water, Mobile Phase B: Acetonitrile.

Example 2: 7-amino-2,3-dimethyl-5-{[(1S)-1-(6-methylpyridin-2-yl)ethyl]amino}pyrazolo [1,5-a]pyrimidine-6-carbonitrile

Example 2 was synthesized similar to example 1 using intermediate I and charily pure (S)-1-(6-methylpyridin-2-yl) ethan-1-amine (CAS:1213399-01-9)

›Abbreviations · 48 of 69

LC-MS Calculated for C 17 H 19 N 7 : 321.39; Observe.: 322.0; [M+H]. 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.19 (br s, 1H), 7.66 (t, J=7.60 Hz, 1H), 7.27 (d, J=7.60 Hz, 1H), 7.14 (d, J=7.60 Hz, 1H), 6.97 (d, J=7.20 Hz, 1H), 5.27 (t, J=7.20 Hz, 1H), 2.23 (s, 3H), 1.94 (s, 3H), 1.50 (d, J=6.80 Hz, 3H). HPLC: 2.39 min; 95.99%; Column: X-Bridge C8(50×4.6) mm, 3.5 μm, Mobile Phase A: 0.1% TFA in water, Mobile Phase B: Acetonitrile. Chiral HPLC: 4.23 min; 100% ee; Column: Lux C4, Mobile Phase A: 0.5% Isopropyl amine in methanol, Mobile phase B: Methanol (30%), Flowrate: 3 mL/min, Injected Volume: 15 μl.

Example 3: (R)-7-Amino-3-ethyl-2-methyl-5-((1-(pyridin-2-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.15 g, 0.0005376 mol) and (R)-1-(pyridin-2-yl) ethan-1-amine (CAS: 45695-03-2, 0.2627 g, 0.00215 mol) in EtOH (15 ml) in a sealed tube, was added TEA (0.6 ml, 0.0043 mol) and the reaction mixture was stirred at 100° C. for 78 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure and the crude material was purified by column chromatography by eluting with 20% EtOAc in hexane to yield the title compound (Example 3) as off-white solid; Yield: (0.038 g, 22%). LC-MS Calculated for C 17 H 19 N 7 is 321.17; Observe: 322.15 [M + +1]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.530-8.541 (d, J=4.4 Hz, 1H), 8.165 (bs, 2H), 7.730-7.773 (m, 1H), 7.449-7.469 (d, J=8 Hz, 1H), 7.241-7.272 (m, 1H), 6.869-6.852 (d, J=6.8 Hz, 1H), 5.229-5.298 (m, 1H), 2.387-2.406 (q, J=7.6 Hz, 2H), 2.229 (s, 3H), 1.496-1.513 (d, J=6.8 Hz, 3H), 1.002-1.040 (t, J=7.2 Hz, 3H). HPLC: 10.35 min; 97.80%, XBridge C18 (4.6*150) mm, 5μ, Mobile Phase A: 10 mM ammonium bicarbonate in water, Mobile Phase B: Acetonitrile: Chiral HPLC: 4.56 min; >97% ee; Column: Chiral Pak IC (250*4.6) mm, 5μ; Mobile Phase A: 0.1% diethylamine in hexane, Mobile phase B: Ethanol (90:10), Flowrate: 1 mL/min, Injected Volume: 5 μl

Example 4: (S)-7-Amino-3-ethyl-2-methyl-5-((1-(pyridin-2-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.15 g, 0.0005376 mol) and (S)-1-(pyridin-2-yl) ethan-1-amine (CAS:27854-90-6; 0.262 g, 0.00215 mol) in EtOH (12 ml) in a sealed tube, was added TEA (0.21 ml, 0.0016 mol) and the reaction mixture was stirred at 100° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with EtOAc (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 30% EtOAc in hexane to yield the title compound (Example 4) as off-white solid; Yield: (0.040 g, 23%). LC-MS: Calculated for C 17 H 19 N 7 is 321.17; Observe: 322.15 [M + +1]. 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.533-8.5444 (d, J=4.4 Hz, 1H), 8.190 (bs, 2H), 7.734-7.772 (t, J=7.2 Hz, 1H), 7.452-7.471 (d, J=7.6 Hz, 1H), 7.242-7.273 (m, 1H), 6.872-6.855 (d, J=6.8 Hz, 1H), 5.249-5.283 (m, 1H), 2.370-2.426 (q, J=8 Hz, 2H), 2.231 (s, 3H), 1.514-1.497 (d, J=6.8 Hz, 3H), 1.004-1.041 (t, J=6.8 Hz, 3H). HPLC: 10.34 min; 96.02%, Column: X Bridge C18 (4.6*150) mm, 5μ, Mobile Phase A: 10 mM Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile. Chiral HPLC: 4.72 min; >97% ee; Column: Chiral Pak IC (250*4.6) mm, 5μ Mobile Phase A: 0.1% Diethylamine in hexane, Mobile phase B: EtOH (90:10). Flowrate: 1 mL/min, Injected Volume: 5 μl

Example 5: (S)-7-Amino-3-ethyl-2-methyl-5-((1-(6-methylpyridin-2-yl) ethyl) amino) pyrazolo [1,5-a] pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.205 g, 0.000735 mol) and (S)-1-(6-methylpyridin-2-yl) ethan-1-amine (CAS: 1213399-01-9, 0.2 g, 0.00147 mol) in IPA (4 ml) in a sealed tube was added TEA (0.32 ml, 0.00172 mol) and the reaction mixture was stirred at 120° C. for 72 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with EtOAc (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 20% EtOAc in hexane to yield the title compound as off-white solid; Yield: (0.0358 g, 17.4%). LC_MS Calc. for C 18 H 21 N 7 is 335.17; Obs.336.20 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.18 (bs, 2H), 7.64 (t, J=7.6 Hz, 1H), 7.6 (d, J=7.6 Hz, 1H), 6.98 (d, J=7.2 Hz, 1H), 5.19 (t, J=6.8 Hz, 1H), 2.42 (q, J=7.2, Hz, 2H), 2.23 (s, 3H), 1.49 (d, J=6.8 Hz, 3H), 1.04 (t, J=7.6 Hz, 3H). HPLC: 11.02 min; 95.75%, Column: X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile

Example 6: 7-amino-2,3-dimethyl-5-{[(6-methylpyridin-2-yl) methyl]amino}pyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.25 g, 0.00094 mol) and (6-methylpyridin-2-yl)methanamine VI (0.46 g, 0.00377 mol) in IPA (10 ml) in a sealed tube, was added TEA (1.3 ml g, 0.00377 mol) and the reaction mixture was stirred at 120° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure and the crude material was purified by column chromatography by eluting with 20% ethyl acetate in hexane to yield the title compound (Example 6) as off-white solid; Yield: (0.1 g, 38%). LC-MS Calculated for C 16 H 17 N 7 is 307.15; Observe. 308.20 [M + +1]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.177 (bs, 2H), 7.633-7.594 (t, J=8 Hz, 1H), 7.232-7.206 (t, J=4.8 Hz, 1H), 7.141-7.096 (m, 2H), 4.616-4.604 (d, J=4.8 Hz, 2H), 2.465 (s, 3H), 2.215 (s, 3H), 1.896 (s, 3H). HPLC: 9.34 min, 97.80%, Column: X Bridge C18 (4.6×150) mm, 5μ, Mobile Phase A: 10 mM Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile.

›Abbreviations · 49 of 69

Example 7: 7-amino-5-{[(6-ethylpyridin-2-yl) methyl]amino}-2,3-dimethylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.100 g, 0.000377 mol) and (6-ethylpyridin-2-yl) methenamine VII (0.102 g, 0.000754 mol) in IPA (10 ml), was added TEA (0.2 ml, 0.00150 mol) and the reaction mixture was heated at 140° C. for 2 days. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (3×20 ml). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 20% ethyl acetate in hexane to yield the title compound (Example 7) as off-white solid; Yield: (0.1 g, 12%). LC-MS Calculated for C 17 H 19 N 7 is 324.18; Observe: 321.17 [M + +1]. 1 HNMR (400 MHz, DMSO-d 6 ): δ8.213 (bs, 2H), 7.665-7.628 (t, J=7.6 Hz, 1H), 7.247 (bs, 1H), 7.169-7.150 (d, J=7.6 Hz, 1H), 7.136-7.117 (d, J=7.6 Hz, 1H), 4.630-4.619 (d, J=4.4 Hz, 2H), 2.777-2.721 (q, J=7.6 Hz, 2H), 2.217 (s, 3H), 1.905 (s, 3H), 1.268-1.233 (t, J=7.6 Hz, 3H). HPLC:10.18 min, 99.44%, Column: X Bridge C18 (4.6×150) mm, 5μ, Mobile Phase A: Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile.

Example 8: 7-amino-2,3-dimethyl-5-{methyl[(6-methylpyridin-2-yl) methyl]amino}pyrazolo [1,5-a] pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.2 g, 0.000754 mol) and N-methyl-1-(6-methylpyridin-2-yl) methanamine VIII (0.123 g, 0.9056 mmol) in IPA (4 ml), was added TEA (0.2 nil, 0.0015 mol) and the reaction mixture was heated at 150° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with DCM (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure to give the desired product (Example 8). The crude material was purified by Prep HPLC. Yield: 25 mg. LC_MS Calculated for C 17 H 19 N 7 : 321.17; Observe: 322.17 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.187 (bs, 2H), 7.656-7.617 (d, J=8 Hz, 1H), 7.128-7.086 (d, J=8.4 Hz, 2H), 4.831 (s, 2H), 3.153 (s, 3H), 2.447 (s, 3H), 2.250 (s, 3H), 1.0 (s, 3H). HPLC: 9.73 min, 98.52%, Column: X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM Ammonium bicarbonate in water, B: ACN.

Example 9: 7-amino-5-({[6-(2-hydroxypropan-2-yl) pyridin-2-yl]methyl}amino)-2,3-dimethylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a stirred solution 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile I (0.15 g, 0.00039 mol) and 2-(6-(aminomethyl) pyridin-2-yl) propan-2-ol X (0.3 g, 0.00180 mol) in IPA (5 ml), was added TEA (0.6 ml, 0.0043 mol) and the reaction mixture was heated at 120° C. for 16 hrs. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (3×20 ml). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by prep HPLC to yield the title compound as off-white solid; Yield: (0.03 g, 15%). LC-MS Calculated for C 18 H 21 N 7 O: 351.18; Observe: 352.25 [M + +1]. 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.202 (bs, 2H), 7.744-7.706 (t, J=7.6 Hz, 1H), 7.529-7.511 (d, J=7.2 Hz, 1H), 7.218-7.199 (d, J=7.6 Hz, 2H), 5.215 (bs, 1H), 4.649-4.637 (d, J=4.8 Hz, 2H), 2.221 (s, 3H), 1.920 (s, 3H), 1.461 (s, 6H). HPLC: 9.42 min, 97.56%, X Bridge C18 (4.6×150) mm, 5μ, Mobile Phase A: 10 mM Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile.

Example 10: 7-Amino-2,3-dimethyl-5-((2-(5-methylpyridin-2-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.25 g, 0.000943 mol) and 2-(5-methylpyridin-2-yl) ethan-1-amine XI (0.192 g, 0.00141 mol) in IPA (5 ml), was added TEA (0.5 ml, 0.00377 mol) and the reaction mixture was heated at 100° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 40% ethyl acetate in hexane to yield the title compound (Example 10) as off-white solid; Yield: (0.050 g, 20%). LC_MS Calculated for C 17 H 19 N 7 : 321.39; Observe: 322.20 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.32 (bs, 1H), 8.09 (bs, 2H),7.52 (d, J=6.8 Hz, 1H), 7.18 (d, J=8.0 Hz, 1H), 6.80-6.78 (m, 1H), 3.67-3.64 (m, 2H), 2.99 (d, J=6.8, Hz, 2H), 2.23 (d, J=12, Hz, 3H),1.96 (s, 1H). HPLC: 9.55 min, 96.36%, Column: X-Bridge C 18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile.

Example 11: 7-amino-5-((2-(5-fluoropyridin-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.2 g, 0.000754 mol) and 2-(5-fluoropyridin-2-yl) ethan-1-amine XII (0.35 g, 0.0025 mol) in IPA (10 ml), was added TEA (0.63 ml, 0.0045 mol) and the reaction mixture was heated at 120° C. for 3 days. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 50% ethyl acetate in hexane to yield the title compound (Example 11) as off-white solid; Yield: (0.070 g, 28%). LC_MS Calculated for C 16 H 16 FN 7 : 325.155; Observe: 326.15 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.45 (d, J=2.40 Hz, 1H), 8.09 (bs, 2H), 7.64 (dt, J=8.8 Hz, 3.2 Hz, 1H), 7.36-7.33 (m, 1H), 6.78-6.67 (m, 1H), 3.67 (q, J=6.8, Hz, 2H), 3.05 (t, J=7.2, Hz, 2H), 2.24 (s, 3H), 1.95 (s, 3H). HPLC: 97.34%, Column: X-Bridge C18 (50×4.6) mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile

›Abbreviations · 50 of 69

Example 12: 7-amino-5-((2-(6-methoxypyridin-2-yl) ethyl)amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.2 g, 0.000754 mol) and 2-(6-methoxypyridin-2-yl) ethan-1-amine XIII (0.353 g, 0.00226 mol) in IPA (4 ml), was added TEA (0.229 g, 0.00226 mol) and the reaction mixture was heated at 150° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 20% ethyl acetate in hexane to yield the title compound (Example 12) as off-white solid; Yield: (0.10 g, 40%). LC_MS Calculated for C 17 H 19 N 7 O: 337.17; Observe: 338.15 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.1 (bs, 2H), 7.624-7.585 (t, J=7.6 Hz, 1H), 6.862-6.844 (d, J=7.2 Hz, 1H), 6.640-6.598 (m, 2H), 3.870 (s, 3H), 3.718-3.703 (m, 2H), 2.982-2.965 (t, J=6.8 Hz, 2H), 2.227 (s, 3H), 1.965 (s, 3H). HPLC: 10.83 min, 98.823%, Column: X-Bridge C 18 (50×4.6) mm, 5 μm, Mobile Phase A: 10 mM Ammonium bicarbonate in water, B: Acetonitrile.

Example 13: 7-amino-5-((2-(6-methylpyridin-2-yl) ethyl)amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.2 g, 0.0007547 mol) and 2-(6-methylpyridin-2-yl) ethan-1-amine XIV (0.411 g, 0.00301 mol) in IPA (10 ml) in a sealed tube, was added TEA (0.6 ml, 0.0045 mol) and the reaction mixture was stirred at 120° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure and the crude material was purified by combi flash by eluting with 15% ethyl acetate in hexane to yield the title compound (Example 13) as off-white solid; Yield: (0.105 g, 43%). LC_MS Calculated for C 17 H 19 N 7 is 321.17; Observe: 322.25 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.11 (bs, 2H), 7.60 (t, J=7.2 Hz, 1H), 7.08 (d, J=8.0 Hz, 2H), 6.93-6.90 (m, 1H), 3.67 (q, J=6.4, Hz, 2H), 2.99 (d, J=6.8, Hz, 2H), 2.22 (s, 3H), 2.48 (s, 3H), 1.96 (s, 3H). HPLC: 98.96%, Column: X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile.

Example 14 and 15 (Racemic): 7-Amino-3-ethyl-2-methyl-5-((1-(pyridin-2-yl) propan-2-yl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a]pyrimidine-6-carbonitrile II (0.150 g, 0.000537 mol) and 1-(pyridin-2-yl) propan-2-amine IX (0.220 g, 0.00484 mol) in IPA (3 ml), was added TEA (0.6 ml, 0.0032 mol) and the reaction mixture was heated in a sealed tube at 120° C. for 60 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 50% ethyl acetate in hexane to yield the title compound (Example 14 and 15 Racemate) as off-white solid; Yield: (0.071 g, 39%). The crude racemic compound obtained (220 mg) was resolved into its two enantiomers by chiral preparative HPLC system.

Example 14: Chiral 7-amino-2,3-dimethyl-5-{[1-(6-methylpyridin-2-yl)propan-2-yl]amino}pyrazolo[1,5-a]pyrimidine-6-carbonitrile. (Isomer 1)

Yield: 64.1 gm: LC-MS Calculated for C 18 H 21 N 7 : 335.19; Observe: 336.20 [M+1] + . 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.10 (bs, 2H), 7.60 (t, J=7.2 Hz, 1H), 7.09 (t, J=8.0 Hz, 2H), 6.91 (d, J=6.8 Hz, 1H), 4.51-4.48 (m, 1H), 3.00-2.96 (m, 2H), 2.50 (s, 3H), 2.22 (s, 3H), 1.96 (s, 3H), 1.14 (d, J=5.6 Hz, 3H); HPLC: 10.31 min, 99.38%, 10.307 min; X Bridge C18 (4.6×150) mm, 5μ, Mobile Phase A: 10 mM Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile. Chiral HPLC: Purity=98.91%, Rt=17.205 min, Chiral Pak IC (250*4.6) mm, 5μ, Mobile Phase A: 0.1% diethyl amine in n-hexane, Mobile Phase B: Isopropanol: dichloromethane (90:5:5); Flow rate: 0.8 mL/min

Example 15: Chiral 7-amino-2,3-dimethyl-5-{[1-(6-methylpyridin-2-yl)propan-2-yl]amino}pyrazolo[1,5-a]pyrimidine-6-carbonitrile. (Isomer 2)

Yield: 55.6 gm; LC-MS Calculated for C 18 H 21 N 7 : 335.19; Observe: 336.20 [M+1] + . 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.10 (bs, 2H), 7.60 (t, J=7.2 Hz, 1H), 7.09 (t, J=8.0 Hz, 2H), 6.91 (d, J=6.8 Hz, 1H), 4.51-4.48 (m, 1H), 3.00-2.96 (m, 2H), 2.50 (s, 3H), 2.22 (s, 3H), 1.96 (s, 3H), 1.14 (d, J=5.6 Hz, 3H); HPLC Purity=99.52%, Rt=10.312 min; X Bridge C18 (4.6×150) mm, 5μ, Mobile Phase A: 10 mM Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile. Chiral HPLC: Purity=100%, Rt=20.769 min, Chiral Pak IC (250*4.6) mm, 5μ, Mobile Phase A: 0.1% diethyl amine in n-hexane, Mobile Phase B: Isopropanol: dichloromethane (90:5:5); Flow rate: 0.8 mL/min

Example 16: 7-amino-5-({2-[6-(2-hydroxypropan-2-yl) pyridin-2-yl]ethyl}amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.15 g, 0.00056 mol) and 2-(6-(2-aminoethyl) pyridin-2-yl) propan-2-ol XV (0.153 g, 0.00084 mol) in IPA (10 ml), was added TEA (0.343 g, 0.00339 mol) and the reaction mixture was heated at 150° C. for 2 days. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with DCM (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by Prep HPLC to get off white title compound (Example 16). Yield: (0.025 g, 12%). LC_MS Calculated for C 19 H 23 N 7 O: 365.20; Observe: 388.1 [M+Na] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.086 (bs, 2H), 7.677 (t, 1H), 7.477-7.458 (d, J=7.6 Hz, 1H), 7.124-7.105 (d, J=7.6 Hz, 1H), 6.609 (t, 1H), 5.163 (s, 1H), 3.726-3.711 (t, J=6 Hz, 2H), 3.046-3.012 (t, J=6.8 Hz, 2H), 2.227 (s, 3H), 1.964 (s, 3H), 1.435 (s, 6H). HPLC: 9.69 min, 98.95%, X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM Ammonium bicarbonate in water, B: Acetonitrile.

›Abbreviations · 51 of 69

Example 17: 7-Amino-3-ethyl-2-methyl-5-((2-(6-methylpyridin-2-yl) ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylation) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.15 g, 0.0005376 mol) and 2-(6-methylpyridin-2-yl) ethan-1-amine XIV (0.292 g, 0.00215 mol) in IPA (5 ml) in a sealed tube, was added TEA (0.23 ml, 0.0016 mol) and the reaction mixture was stirred at 120° C. for 16 h. The progress of the reaction was monitored by TLC (non-polar spot). After completion, the reaction mixture was evaporated under reduced pressure and the crude material was purified by column chromatography by eluting with 40% ethyl acetate in hexane to yield the title compound (Example 17) as off-white solid; Yield: (0.056 g, 31%). LC_MS Calculated for C 18 H 21 N 7 : 335.17; Obs.336.15 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.10 (bs, 1H), 7.60 (t, J=7.6 Hz, 1H), 7.08 (dd, J=8.0, 3.6 Hz, 2H), 6.92 (t, J=4.8 Hz, 1H), 3.66 (q, J=6.0, Hz, 2H), 2.99 (t, J=6.8, Hz, 2H), 2.50-2.43 (m, 5H), 2.24 (s, 3H), 1.24 (t, J=7.6 Hz, 3H). _HPLC: 99.57%, X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile.

Example 18: 7-Amino-3-ethyl-2-methyl-5-((2-(5-methylpyridin-2-yl) ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.15 g, 0.00049 mol) and 2-(5-methylpyridin-2-yl) ethan-1-amine XI (0.166 g, 0.00122 mol) in IPA (10 ml), was added TEA (0.342 ml, 0.000725 mol) and the reaction mixture was heated at 100° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 40% ethyl acetate in hexane to yield the title compound (Example 18) as off-white solid; Yield: (0.050 g, 31%). LC_MS Calculated. for C 18 H 21 N 7 . 335.17; Obs.336.15 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.32 (s, 1H), 8.09 (bs, 2H), 7.52 (d, J=6.4 Hz, 1H), 7.17 (d, J=8.0 Hz, 1H), 6.80 (t, J=5.2 Hz, 1H), 3.67-3.62 (m, 2H), 2.99 (t, J=7.2, Hz, 2H), 2.43 (d, J=7.6, Hz, 2H), 2.25 (d, J=4.0 Hz, 6H), 1.11 (t, J=7.6 Hz, 3H). HPLC: 98.81%, Zorbax SB-C8 (4.6*75) mm, 3.5μ, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile.

Example 19: 7-Amino-3-ethyl-5-((2-(5-methoxypyridin-2-yl) ethyl) amino)-2-methylpyrazolo [1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.2 g, 0.000716 mol) and 2-(5-methoxypyridin-2-yl) ethan-1-amine XIX (0.435 g, 0.00286 mol) in IPA (5 ml), was added TEA (0.6 ml, 0.0043 mol) and the reaction mixture was heated at 120° C. for 3 days. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 50% ethyl acetate in hexane to yield the title compound (Example 19) as off-white solid; Yield: (0.080 g, 31.8%). LC_MS Calculated for C 18 H 21 N 7 O: 351.18; Observe: 352.20 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.20 (d, J=2.8 Hz, 1H), 8.08 (bs, 2H), 7.33 (dd, J=8.4, 2.8 Hz, 1H), 7.21 (d, J=8.4 Hz, 1H), 6.75 (t, J=5.6 Hz, 1H), 3.79 (s, 3H), 3.64 (q, J=6.8, Hz, 2H), 2.98 (t, J=7.2, Hz, 2H), 2.5-2.40 (m, 2H), 2.24 (s, 3H), 1.12 (t, J=7.6 Hz, 3H). HPLC: 99.23%, X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile

Example 20: 7-Amino-3-ethyl-5-((2-(6-methoxypyridin-2-yl) ethyl) amino)-2-methylpyrazolo [1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.2 g, 0.000716 mol) and 2-(6-methoxypyridin-2-yl) ethan-1-amine XIII (0.230 g, 0.00151 mol) in IPA (5 ml), was added TEA (0.6 ml, 0.0043 mol) and the reaction mixture was heated at 120° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by flash column chromatography by eluting with 50% ethyl acetate in hexane to yield the title compound (Example 20) as off-white solid; Yield: (0.097 g, 38.6%). LC_MS Calculated. for C 18 H 21 N 7 O is 351.18; Observe: 352.25 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.09 (bs, 2H), 7.60 (t, J=8.0 Hz, 1H), 6.84 (d, J=7.20 Hz, 1H), 6.64-6.61 (m, 2H), 3.86 (s, 3H), 3.69 (q, J=6.0, Hz, 2H), 2.96 (t, J=7.2, Hz, 2H), 2.46 (q, J=7.2 Hz, 2H), 2.24 (s, 3H), 1.12 (t, J=7.6 Hz, 3H). HPLC: 98.52%, X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile.

Example 21: 7-Amino-3-ethyl-5-((2-(5-fluoropyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.2 g, 0.000716 mol) and 2-(5-fluoropyridin-2-yl) ethan-1-amine XII (0.4 g, 0.00286 mol) in IPA (5 ml), was added TEA (0.6 ml, 0.0043 mol) and the reaction mixture was heated at 120° C. for 3 days. The progress of the reaction was monitored by TLC (non-polar spot). After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 50% ethyl acetate in hexane to yield the title compound (Example 21) as off-white solid; Yield: (0.050 g, 20.5%). LC_MS Calculated for C 17 H 18 FN 7 : 339.16; Observe. 340.20 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.45 (d, J=2.40 Hz, 1H), 8.10 (bs, 2H), 7.64 (dt, J=8.4, 2.8 Hz, 1H), 7.35-7.31 (m, 1H), 6.75 (d, J=5.2 Hz, 1H), 3.67 (q, J=6.8, Hz, 2H), 3.05 (t, J=7.2, Hz, 2H), 2.44 (q, J=7.2 Hz, 2H), 2.24 (s, 3H), 1.11 (t, J=7.6 Hz, 3H), HPLC: 98.34%, X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile.

›Abbreviations · 52 of 69

Example 22 and 23 (Racemate): 7-Amino-3-ethyl-2-methyl-5-((1-(pyridin-2-yl) propan-2-yl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (Racemic)

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.150 g, 0.000537 mol) and 1-(pyridin-2-yl) propan-2-amine (CAS: 51038-40-5, 0.220 g, 0.00484 mol) in IPA (3 ml), was added TEA (0.6 ml, 0.0032 mol) and the reaction mixture was heated in a sealed tube at 120° C. for 60 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 50% ethyl acetate in hexane to yield the title compound as off-white solid; Yield: (0.071 g, 39%). Then the racemic compound of (Example 22 and 23) (71 mg) was resolved into its two enantiomers by chiral preparative HPLC system

Example 22: Chiral 7-Amino-3-ethyl-2-methyl-5-((1-(pyridin-2-yl) propan-2-yl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile. (Isomer 1)

Yield: 0.0154 g: LC_MS Calculated for C 18 H 21 N 7 : 335.19; Observe: 336.20 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.48 (d, J=4.4 Hz, 1H), 8.07 (bs, 2H), 7.70 (dt, J=7.6 Hz, 1.6 Hz, 1H), 7.30 (d, J=7.6 Hz, 1H), 7.20 (t, J=7.2 Hz, 1H), 6.71 (d, J=7.6 Hz, 1H), 4.51-4.48 (m, 1H), 3.11-3.06 (m, 1H), 2.96-2.91 (m, 1H), 2.45 (q, J=7.6 Hz, 2H), 2.23 (s, 3H) 1.16 (d, J=6.4, Hz, 3H), 1.10 (t, J=7.6 Hz, 3H). HPLC: 97.85%, Column: X-Bridge C8 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 0.1% TFA in water, Mobile Phase B: Acetonitrile.

Example 23: Chiral 7-Amino-3-ethyl-2-methyl-5-((1-(pyridin-2-yl) propan-2-yl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile. (Isomer 2)

Yield: 0.0164 g: LC_MS Calculated for C 8 H 21 N 7 : 335.19; Observe: 336.20 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.48 (d, J=4.0 Hz, 1H), 8.07 (bs, 2H), 7.69 (t, J=7.6 Hz, 1H), 7.31 (d, J=7.6 Hz, 1H), 7.20 (t, J=6.8 Hz, 1H), 6.71 (d, J=7.6 Hz, 1H), 4.51-4.48 (m, 1H), 3.11-3.06 (m, 1H), 2.96-2.91 (m, 1H), 2.45 (q, J=7.6 Hz, 2H), 2.23 (s, 3H) 1.16 (d, J=6.4, Hz, 3H), 1.10 (t, J=7.2 Hz, 3H). HPLC: 98.38%, X-Bridge C8 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 0.1% TFA in water, Mobile Phase B: Acetonitrile.

Example 24: 7-Amino-3-ethyl-2-methyl-5-((1-(6-methylpyridin-2-yl) propan-2-yl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methylthio) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.5 g, 0.00179 mol) and 1-(6-methylpyridin-2-yl) propan-2-amine IX (0.430 g, 0.00284 mol) in IPA (10 ml), was added TEA (1.49 ml, 0.01075 mol) and the reaction mixture was heated at 120° C. for 3 days. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by flash column chromatography by eluting with 10% ethyl acetate in hexane to yield the title compound (Example 24) as racemic off-white solid; Yield: (0.350 g, 56%).). LC_MS Calculated for C 19 H 23 N 7 is 349.2; Observe: 350.20 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.11 (brs, 2H), 7.59 (t, J=7.6 Hz, 1H), 7.09 (t, J=8.80 Hz, 2H), 6.93 (d, J=7.2, Hz, 1H), 4.5-4.4 (m, 1H), 2.9-2.93 (m, 2H), 2.49 (s, 3H), 2.43 (q, J=7.60 Hz, 2H), 2.23 (s, 3H), 1.15 (d, J=6.8, Hz, 3H), 1.10 (t, J=7.6, Hz, 3H HPLC: 95.52%, X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water B: Acetonitrile.

Example 25: 7-amino-3-ethyl-5-({2-[6-(2-hydroxypropan-2-yl) pyridin-2-yl]ethyl}amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.15 g, 0.000537 mol) and 2-(6-(2-aminoethyl) pyridin-2-yl) propan-2-ol XV (0.145 g, 0.000804 mol) in IPA (10 ml), was added TEA (0.326 g, 0.00322 mol) and the reaction mixture was heated at 150° C. for 2 days. The progress of the reaction was monitored by TLC. After completion, reaction mixture was concentrated to dryness under reduced pressure. the reaction mixture was diluted with water (20 ml) and extracted with Ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure to get the desired product (Example 25). The crude material was purified by Prep HPLC. Yield: (0.03 g, 14.7%). LC_MS Calculated for C 20 H 25 N 7 O is 379.21; Observe: 380.25 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.104 (bs, 2H), 7.695-7.657 (t, J=7.2 Hz, 1H), 7.478-7.459 (d, J=7.6 Hz, 1H), 7.113-7.094 (d, J=7.6 Hz, 1H), 6.659 (t, 1H), 5.170 (s, 1H), 3.709-3.694 (t, J=6 Hz, 2H), 3.044-3.011 (t, J=6.8 Hz, 2H), 2.455 (t, 2H), 2.247 (s, 3H), 1.434 (s, 6H), 1.145-1.108 (t, J=7.2 Hz, 3H). HPLC: 98.77%, X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM Ammonium bicarbonate in water, B: Acetonitrile.

Example 26: 7-amino-3-(cyclopropylmethyl)-2-methyl-5-((2-(6-methylpyridin-2-yl) ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

A solution of 7-amino-3-(cyclopropyl methyl)-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile V (0.212 g, 0.000697 mol) and 2-(6-methylpyridin-2-yl) ethan-1-amine XIV (0.190 g, 0.00139 mol) in IPA (5 mL) was purged with N 2 gas for 10 min and TEA (0.58 ml, 0.00418 mol) was added. The reaction mixture was heated at 120° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was concentrated under reduced pressure and the residue was dissolved in ethyl acetate (50 mL). The resulting was extracted with water (20 mL×3). The organic layer was washed with brine and dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure. The crude material was purified by silica gel (combi flash) column chromatography by eluting with 40% ethyl acetate in n-hexane to yield the title compound (Example 26) as an off-white solid; Yield: (0.140 g, 56%). LC_MS Calculated for C 20 H 23 N 7 is 361.20; Observe: 362.20 [M + +H]. 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.12 (bs, 2H), 7.6 (t, J=7.6 Hz, 1H), 7.07 (t, J=7.2 Hz, 2H), 6.98-6.92 (m, 1H), 3.65 (q, J=6.0, Hz, 2H), 2.98 (t, J=6.8, Hz, 2H), 2.4 (d, J=6.8 Hz, 2H), 2.2 (s, 3H), 1.0-0.95 (m, 1H), 0.35 (d, J=7.6 Hz, 2H), 0.18 (d, J=4.4 Hz, 2H). HPLC: 11.10 min, 95.83%, X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile.

›Abbreviations · 53 of 69

Example 27: 7-amino-3-(isopropylmethyl)-2-methyl-5-((2-(6-methylpyridin-2-yl)ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-isobutyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile IV (0.150 g, 0.00049 mol) and 2-(6-methylpyridin-2-yl)ethan-1-amine XIV (0.2 g, 0.00146 mol) in isopropanol (15 ml) in a sealed tube, was added TEA (0.41 ml, 0.0029 mol) and the reaction mixture was stirred at 120° C. for 16 h. The progress of the reaction was monitored by TLC (less-polar spot). After completion, the reaction mixture was evaporated under reduced pressure and the crude material was purified by column chromatography by eluting with 0-25% ethyl acetate in n-hexane to yield the title compound (Example 27) as an off-white solid; Yield: (0.125 g, 71%). LC_MS Calculated for C 20 H 25 N 7 is 363.22; Observe. 364.20 [M + +H]. 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.11 (bs, 2H), 7.60 (t, J=8.0 Hz, 1H), 7.01 (t, J=7.6 Hz, 2H), 6.92 (s, 1H), 3.64-3.61 (m, 2H), 3.00 (t, J=7.2 Hz, 2H), 2.47 (s, 3H), 2.32 (d, J=7.2 Hz, 2H), 2.23 (s, 3H), 1.81-1.95 (m, 1H) 0.87 (d, J=6.8 Hz, 6H); HPLC: 11.66 min, 97.26%, X-Bridge C18 (50×4.6) mm, mm, 5 μm, Mobile Phase A: 10 mM Ammonium bicarbonate in water, B: Acetonitrile.

Example 28: 7-amino-3-(isopropyl)-2-methyl-5-((2-(6-methylpyridin-2-yl)ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-isopropyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile III (0.2 g, 0.00068 mol) and 2-(6-methylpyridin-2-yl)ethan-1-amine XIV (0.278 g, 0.00204 mol) in IPA (2 ml) in a sealed tube, was added TEA (0.278 g, 0.00274 mol) and the reaction mixture was stirred at 120° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was evaporated under reduced pressure and the crude material was purified by column chromatography by eluting with 20% ethyl acetate in hexane to yield the title compound (Example 28) as off-white solid; Yield: (0.068 g, 29%). LC-MS Calculated for C 19 H 23 N 7 349.20; Observe: 350.20 [M + +1]. 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.063 (bs, 2H), 7.612-7.574 (t, J=7.6 Hz, 1H),7.088-7.047 (t, J=8.4 Hz, 2H), 6.893 (bs, 1H), 3.657-3.644 (m 2H), 2.995-2.979 (t, J=6.4 Hz, 2H), 2.942-2.908 (m, 1H), 2.460 (s, 3H), 2.257 (s, 3H), 1.303-1.286 (d, J=6.8 Hz, 6H). HPLC: 11.28 min, 98.85%, Column: X Bridge C18 (4.6×150) mm, 5μ, Mobile Phase A: 10 mM Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile.

Example 29: 7-amino-5-{[2-(1-ethyl-1H-pyrazol-3-yl) ethyl]amino}-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.2 g, 0.000943 mol) and 2-(1-ethyl-1H-pyrazol-3-yl) ethan-1-amine XVI (0.387 g, 0.00282 mol) in IPA (3 ml), was added TEA (0.381 g, 0.00377 mol) and the reaction mixture was heated at 120° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 20% ethyl acetate in hexane to yield the title compound (Example 29) as off-white solid; Yield: (0.057 g, 18%). LC-MS Calculated for C 17 H 19 N 7 : 324.39; Observe: 3325.2 [M + +1]. 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.11 (bs, 2H), 7.611-7.608 (d, J=1.2 Hz, 1H), 6.642 (m, 1H), 6.071-6.067 (d, J=1.6 Hz, 1H), 4.092-4.037 (q, J=7.6 Hz, 2H), 3.590-3.575 (t, J=6 Hz, 2H), 2.840-2.804 (t, J=7.2 Hz, 2H), 2.229 (s, 3H), 1.966 (s, 3H), 1.369-1.332 (t, J=7.6 Hz, 3H). HPLC: 9.28 min, 99.42%, X Bridge C18 (4.6×150) mm, 5μ, Mobile Phase A: 10 mM Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile.

Example 30: 7-amino-3-ethyl-5-{[2-(1-ethyl-1H-pyrazol-3-yl)ethyl]amino}-2-methylpyrazolo [1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.25 g, 0.000925 mol) and 2-(1-Methyl-1H-pyrazol-3-yl) ethan-1-amine XVII (0.343 g, 0.00278 mol) in IPA (5 ml), was added TEA (0.374 g, 0.00370 mol) and the reaction mixture was heated at 120° C. for 24 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 20% ethyl acetate in hexane to yield the title compound (Example 30) as off-white solid; Yield: (0.037 g, 12%). LC-MS Calculated for C 16 H 20 N 8 : 324.18; Observe: 325.18 [M++1]. 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.094 (bs, 2H), 7.560-7.556 (d, J=1.6 Hz, 1H), 6.685-6.658 (t, J=5.2 Hz, 1H), 6.054-6.049 (d, J=2 Hz, 1H), 3.772 (s, 3H), 3.582-3.533 (t, J=6.8 Hz, 2H), 2.830-2.794 (t, J=7.2 Hz, 2H), 2.446-2.428 (q, 2H), 2.247 (s, 3H), 1.136-1.099 (t, J=7.2 Hz, 3H). HPLC: 9.49 min, 97.43%, X Bridge C18 (4.6×150) mm, 5μ, Mobile Phase A: 10 mM Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile.

Example 31: 7-amino-5-({2-[1-(1-hydroxy-2-methylpropan-2-yl)-1H-pyrazol-3-yl]ethyl}amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.2 g, 0.00075 mol) and 2-(3-(2-aminoethyl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol XVIII (0.345 g, 0.00188 mol) in IPA (5 ml), was added TEA (0.229 g, 0.00226 mol) and the reaction mixture was heated at 120° C. for 16 h. The progress of the reaction was monitored by TLC. After completion, the reaction mixture was diluted with water (20 ml) and extracted with ethyl acetate (20 ml×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 20-50% ethyl acetate in hexane to yield the title compound (Example 31) as off-white solid; Yield: (0.1 g, 36%). LC-MS Calculated for C 18 H 24 N 8 O 368.21; Observe: 369.21 [M + +1]. 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.105 (bs, 1H), 7.650 (s, 1H), 6.593-6.581 (t, 1H), 6.062 (s, 1H), 4.901-4.874 (t, J=5.6 Hz, 1H), 3.616-3.548 (m, 4H), 2.840-2.805 (t, J=6.8 Hz, 2H), 2.229 (s, 3H), 1.968 (s, 3H), 1.439 (s, 6H). HPLC: 8.93 min, 97.57%, Column: X Bridge C18 (4.6×150) mm, 5μ, Mobile Phase A: 10 mM Ammonium bicarbonate in water, Mobile Phase B: Acetonitrile.

›Abbreviations · 54 of 69

Example 32: 7-amino-3-ethyl-5-((2-(1-(1-hydroxy-2-methylpropan-2-yl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (2.0 g, 0.0071 mol), 2-(3-(2-aminoethyl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol XXIV (3.2 g, 0.017 mol), IPA (5 mL) and triethylamine (6.0 mL, 0.043 mol) were taken in a seal tube and the reaction mixture was stirred at 150° C. for 12 h. The reaction was monitored by TLC. The reaction mixture was diluted with water (20 mL) and extract with ethyl acetate (3×20 mL). The combined organic layer was dried over Na 2 SO 4 and evaporated under reduced pressure to get crude product. Crude product was purified by biotage column chromatography using silica gel (230-400) and 10-30% ethyl acetate in hexane as eluent to afford the title compound Example 32 as an off white solid. Yield: 1.048 g, 38.8%. LCMS Calculated. for C 19 H 26 N 8 O is 382.47; Observed. 383.1 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.09 (bs, 2H), 7.64 (d, J=2.0 Hz, 1H), 6.59 (t, J=5.2 Hz, 1H), 6.05 (d, J=2.4 Hz, 2H), 4.88 (m, 1H), 3.59-3.54 (m, 4H),2.82 (t, J=6.8 Hz, 2H), 2.5-2.4 (m, 2H), 2.47 (s, 3H), 1.437 (s, 6H), 1.12 (t, J=7.2 Hz, 3H). HPLC: 99.11%, X-Bridge C18 (50×4.6) mm, 5 μm, Mobile Phase A: 10 mM ammonium bicarbonate in water, B: Acetonitrile

Example 33: 7-amino-3-chloro-5-((2-(6-(hydroxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Synthesis of 7-amino-3-ethyl-5-((2-(6-(methoxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile. XXIa

A stirred solution of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl)pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX and 7-amino-3-chloro-2-methyl-5-(methyl sulfinyl)pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXa (1.6 g, 0.056 mol) in IPA (60 mL) was added 2-(6-(methoxy methyl) pyridin-2-yl) ethan-1-amine XXI (3.7 g, 0.022 mol) and TEA (4.7 mL, 0.034 mol) in a sealed tube under argon atmosphere and the reaction mixture was stirred at 160° C. for 48 hours. The progress of the reaction was monitored by TLC. After completion of the reaction mixture was evaporated under reduced pressure and the crude material was purified by gravity column and compound eluted in 25-30% Ethyl acetate in DCM. To yield the desired compound XXIa as off-white solid; Yield: 1.2 g, 57%. LCMS Calculated for C 7 H 18 ClN 7 O is 371.13; Observed. 372.15 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.42 (bs, 2H), 7.74 (t, J=8.0 Hz, 1H), 7.26-7.19 (m, 3H), 4.51 (s, 2H), 3.69 (t, J=5.6 Hz, 2H), 3.36 (s, 3H), 3.03 (t, J=5.6 Hz, 2H), 2.27 (s, 3H).

Synthesis of 7-amino-3-ethyl-5-((2-(6-(hydroxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

A stirred solution of 7-amino-3-ethyl-5-((2-(6-(methoxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile XXIa (1.2 g, 0.0032 mol) in DCM (3 ml) was cool to 0° C. added BBr 3 (0.46 mL, 0.0048 mol) dropwise and the reaction mixture was stirred at 0° C. for 3 hours. The progress of the reaction was monitored by TLC. The reaction mixture quenched with NaHCO 3 solution (100 mL) and extracted with DCM (3×200 mL). The combined organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated to afford crude product. Crude compound obtained was purified by gravity column using 230-400 mesh silica gel. The desired product eluted at 35-40% ethyl acetate in hexane to afford title compound (Example 33) as off-white solid. Yield: 0.631 g, 53%. LCMS Calculated for C 16 H 16 ClN 7 O is 357.11; Observed. 358.15 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.40 (bs, 2H), 7.72 (t, J=7.6 Hz, 1H), 7.32 (d, J=7.6 Hz, 1H), 7.20-7.15 (m, 2H), 5.36 (t, J=5.6 Hz, 1H), 4.58 (d, J=5.6 Hz, 2H), 3.70-3.68 (m, 2H), 3.02 (t, J=7.2 Hz, 2H), 2.27 (s, 3H). HPLC: 97.91%, INT ODS 3V-C18 (4.6×250) mm, 5 μm, Mobile phase A: 0.1% Formic acid in water, B: Acetonitrile

Example 34: 7-amino-3-chloro-5-((2-(6-(1-(hydroxymethyl) cyclopropyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a mixture of 7-amino-3-chloro-2-methyl-5-(methylsulfonyl)pyrazolo[1,5-a]pyrimidine-6-carbonitrile XX and 7-amino-3-chloro-2-methyl-5-(methyl sulfinyl) pyrazolo [1,5-a] pyrimidine-6-carbonitrile XXa (0.25 g, 1 eq, 0.88 mmol) in isopropanol (10 mL) was added (1-(6-(2-aminoethyl) pyridin-2-yl)cyclopropyl)methanol XXII (0.34 g, 2 eq, 1.8 mmol) followed by triethylamine (0.27 g, 0.37 mL, 3 eq, 2.6 mmol) in a sealed tube and the resulting mixture was purged with N 2 for 10 min and caped. Then the reaction mixture was heated at 140° C. for 24 hours. Then the progress of the reaction was monitored by TLC for the absence of starting material. The reaction was cooled to RT and concentrated under vacuo. The residue obtained was added ethyl acetate (20 mL) and water (20 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, concentrated under vacuo to afford light brown viscous liquid. The crude compound was subjected to silica gel (230-400) column chromatography using ethyl acetate (0-80%) in n-hexane. The peak eluted with 45% Ethyl acetate in hexane was concentrated to afford the title compound (Example 34) as an off white solid. Yield: 0.04 g, 11%. LCMS Calculated. for C 19 H 20 ClN 7 O is 397.14; Observed. 398.20 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.38 (bs, 2H), 7.60 (t, J=7.6 Hz, 1H), 7.30 (d, J=8.0 Hz, 1H), 7.03 (d, J=7.6 Hz, 1H), 6.94 (t, J=6.0 Hz, 1H), 4.71 (t, J=6.0 Hz, 1H), 3.75-3.67 (m, 4H), 2.97 (t, J=7.2 Hz, 2H), 2.28 (s, 3H), 1.13-1.10 (m, 2H), 0.87-0.85 (m, 2H), HPLC: 99.63%, X-Bridge C18 (50×4.6) mm, 5 μm, Mobile Phase A: 10 mM Ammonium bicarbonate in water, B: Acetonitrile.

Example 35: 7-amino-3-chloro-5-((2-(1-(2-hydroxyethyl)-4,5-dihydro-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

Synthesis of 7-amino-3-chloro-5-((2-(1-(2-methoxyethyl)-4,5-dihydro-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (XXIIIc)

›Abbreviations · 55 of 69

7-amino-3-chloro-5-((2-(1-(2-hydroxyethyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methyl pyrazolo [1,5-a] pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-chloro-5-((2-(1-(2-methoxyethyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile XXIIIc (0.160 g, 4.268 mol) in DCM (15 mL), slowly added BBr 3 (0.06 mL, 6.4 mol) at 0° C., and then reaction mixture was stirred at RT for 1 h. The reaction mixture was diluted with water (20 mL) and extracted with EtOAc (3×20 mL). the combined organic layer was dried over anhydrous Na 2 SO 4 and evaporated under reduced pressure to get crude product. The crude product obtained was purified by biotage column chromatography (12 g silica gel column) and by eluting with methanol in DCM (0-2%). to afford title compound (Example 35) as an off white solid. Yield: 0.066 g, 43%. LCMS Calculated. for C 15 H 19 ClN 8 O is 360.81; Observed. 361.25 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.39 (bs, 2H), 7.58 (S, 1H), 7.02 (m, 1H), 6.07 (s, 1H), 4.83 (t, J=10.8 Hz, 1H), 4.07 (t, J=11.2 Hz, 2H), 3.73-3.70 (m, 2H) 3.62-3.57 (M, 2H), 2.82 (t, J=7.2 Hz, 2H), 2.27 (s, 3H). HPLC: 98.51%, YMC-Pack ODS-AQ (4.6×250) mm, 5 μm, Mobile Phase A: 10 mM ammonium acetate in water, B: Acetonitrile

Example 36: 7-amino-3-chloro-5-((2-(1-(1-hydroxy-2-methylpropan-2-yl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a mixture of 7-amino-3-chloro-2-methyl-5-(methylsulfonyl)pyrazolo[1,5-a]pyrimidine-6-carbonitrile XX and 7-amino-3-chloro-2-methyl-5-(methyl sulfinyl) pyrazolo [1,5-a] pyrimidine-6-carbonitrile XXa (0.25 g, 1 eq, 0.88 mmol) in isopropanol (10 mL) was added 2-(3-(2-aminoethyl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol XVIII (0.096 g, 5.25 mol), triethylamine (0.1 mL, 0.0011 mol) were taken in a seal tube and the resulting reaction mixture was stirred at 150° C. for 12 h. Then the progress of the reaction was monitored by TLC. The reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (3×20 mL). The combined organic layer was dried over anhydrous Na 2 SO 4 and evaporated under reduced pressure to get crude product. Then the obtained crude product was purified by biotage column chromatography using silica gel (230-400) and 1% methanol in DCM as eluent to afford title compound (Example 36) as an off white solid. Yield: 0.055 g, 40%. LCMS Calculated. for C 16 H 21 ClN 8 O is 388.86; Observed. 389.2 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ): δ 7.46 (d, J=2.4 Hz, 1H), 6.11 (d, J=2.0 Hz, 1H), 6.04 (bs, 2H), 5.78 (bs, 1H), 3.88-3.83 (m, 4H), 3.49-3.47 (m, 1H), 2.95 (d, J=6.4 Hz, 2H) 2.3 (s, 3H), 1.55 (s, 6H). HPLC: 97.5%, INT ODS 3V-C18 (4.6×250) mm, 5 μm, Mobile Phase A: 0.1% Formic acid in water B: Acetonitrile

Example 37: 7-amino-3-chloro-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a mixture of 7-amino-3-chloro-2-methyl-5-(methylsulfonyl)pyrazolo[1,5-a]pyrimidine-6-carbonitrile XX and 7-amino-3-chloro-2-methyl-5-(methyl sulfinyl) pyrazolo [1,5-a] pyrimidine-6-carbonitrile XXa (0.25 g, 1 eq, 0.88 mmol) in isopropanol (10 mL) was added a mixture of (1-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopropyl)methanol (0.30 g, 0.0017 mol), XXIV (0.3 g, 0.0007 mol), triethylamine (0.425 g, 0.0042 mol) were taken in a seal tube and purged with N 2 for 10 min. Then the resulting reaction mixture was heated at 140° C. for 24 hours. The progress of the reaction was monitored by TLC. The reaction was cooled to rt and concentrated under vacuo. To the residue add ethyl acetate (20 mL) and water (20 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate. The organic layer was concentrated under vacuo and the crude compound was purified by combi-flash (230-400; 40 g column) using 80% ethyl acetate and hexane to afford the title compound (Example 37) as an off white solid. Yield: 0.1 g, 36.9%. LCMS Calculated. for C 17 H 19 ClN 8 O is 386.14; Observed. 387.15 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.40 (bs, 2H), 7.62 (d, J=2.0 Hz, 1H), 6.94 (t, J=5.6 Hz, 1H), 6.06 (d, J=2.0 Hz, 1H), 4.88 (t, J=5.6 Hz, 1H), 3.63-3.58 (m, 4H), 2.82 (t, J=7.6 Hz, 2H), 2.28 (s, 3H), 1.09-1.07 (m, 2H), 0.97-0.95 (m, 2H). HPLC: 99.14%, X-Bridge C18 (50×4.6) mm, 5 μm, Mobile Phase A: 10 mM Ammonium bicarbonate in water, B: Acetonitrile.

Example 38: 7-amino-3-chloro-5-((2-(6-(2-(hydroxymethyl) cyclopropyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a mixture of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile XX and 7-amino-3-chloro-2-methyl-5-(methyl sulfinyl) pyrazolo [1,5-a] pyrimidine-6-carbonitrile XXa (0.25 g, 0.88 mmol) in isopropanol (10 mL) was added triethylamine (0.27 g, 2.6 mmol) and (2-(6-(2-aminoethyl) pyridin-2-yl)cyclopropyl)methanol XXV (0.3 g, 2 mmol) in a seal tube. The resulting reaction mixture was purged with N 2 for 10 min and heated at 140° C. for 24 hours. Then the progress of the reaction was monitored by TLC analysis. The reaction was cooled to room temperature and concentrated under vacuo. To the residue was added ethyl acetate (20 mL) and water (20 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was purified by prep-HPLC to get the title compound (Example 38) as white solid using X-bridge Prep C18 (250×19) mm, 5.0 μm, mobile phase A: 10 mm ammonium bi-carbonate in water; B: acetonitrile. Yield: 0.04 g, 11%. LCMS Calculated. for C 19 H 20 ClN 7 O is 397.14; Observed. 398.15 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.39 (bs, 2H), 7.54 (t, J=7.2 Hz, 1H), 7.06-7.00 (m, 3H), 4.56 (t, J=5.2 Hz, 1H), 3.70-3.65 (m, 2H), 3.50-3.44 (m, 1H), 3.40-3.33 (m, 1H), 2.96 (t, J=6.8 Hz, 2H), 2.28 (s, 3H), 1.99-1.95 (m, 1H), 1.58-1.55 (m, 1H), 1.10-1.06 (m, 1H), 0.89-0.84 (m, 1H). HPLC: 97.87%, INT ODS 3V-C18 (4.6×250) mm, 5 μm. Mobile Phase A: 0.1% formic acid in water, B: Acetonitrile.

›Abbreviations · 56 of 69

Example 39: 7-amino-3-ethyl-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

In a seal tube, a solution of (1-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopropyl) methanol XXIV (260 mg, 0.0009 mol) and 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (420 mg, 0.0023 mol) in isopropanol (15 mL) was taken and added triethylamine (0.7 mL, 0.0055 mol). The resulting reaction mixture was purged with N 2 for 10 min. The seal tube was heated at 140° C. for 48 hours. The progress of the reaction was monitored by TLC. The reaction was cooled to room temperature and concentrated under vacuo. To the residue was added ethyl acetate and water. The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuum. The crude compound was purified by preparative HPLC to obtain the title compound (Example 39) as yellow solid. Yield: (0.025 g, 7.1%). LCMS Calculated. for C 19 H 24 N 8 O is 380.21; Observed. 381.25 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.10 (bs, 1H), 7.61 (d, J=1.6 Hz, 1H), 6.59 (s, 1H), 6.04 (d, J=1.6 Hz, 1H), 4.87 (t, J=5.6 Hz, 1H), 3.59-3.54 (m, 3H), 2.80 (t, J=6.8 Hz, 2H), 2.5 (m, 2H), 2.47 (s, 3H), 1.13-1.07 (m, 5H), 0.97-0.94 (m, 2H). HPLC: 97.16%, X-Bridge C18 (50×4.6) mm, 5 μm, Mobile Phase A: 10 mM Ammonium bicarbonate in water, B: Acetonitrile.

Example 40: 7-amino-5-((2-(6-ethylpyridin-2-yl) ethyl) amino)-2,3-dimethyl pyrazolo [1,5-a] pyrimidine-6-carbonitrile

To a solution of 2-(6-ethylpyridin-2-yl) ethan-1-amine XXVI (0.74 g, 4.9 mmol) and 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.65 g, 2.5 mmol) in isopropanol (10 mL) was added triethylamine (2.0 mL, 15 mmol). The resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to room temperature and the reaction mixture was concentrated under vacuo. To the residue were added ethyl acetate (50 mL) and water (50 mL) and mixed well. The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was purified by combi-flash (230-400) silica gel column chromatography using ethyl acetate in n-hexane and peak eluted with 15% ethyl acetate in n-hexane was concentrated to afford the desired title compound (Example 40) as an off-white solid. Yield: 0.3 g (34%); LC_MS Calculated. for C 18 H 22 N 7 is 336.20, Observed, 336.30; [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.11 (s, 2H), 7.63 (t, J=7.6 Hz, 1H), 7.11 (d, J=7.6 Hz, 2H), 6.86 (t, J=5.2 Hz, 1H), 3.71-3.67 (m, 2H), 3.01 (t, J=6.8 Hz, 2H), 5.47 (d, J=10.8 Hz, 1H), 3.74 (t, J=6.8 Hz, 2H), 2.75 (q, J=7.6 Hz, 2H), 2.23 (s, 1H), 1.97 (s, 3H), 1.23 (t, J=7.6 Hz, 2H); HPLC Purity=98.00%, INT ODS 3V-C18 (4.6×250) mm, 5μ; Mobile Phase A: 0.1% Formic acid in water: Mobile Phase B: Acetonitrile; Retention time=7.721 min.

Example 41: 7-amino-5-((2-(3-fluoro-6-methylpyridin-2-yl) ethyl)amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of 2-(3-fluoro-6-methylpyridin-2-yl) ethan-1-amine XXVII (203 mg, 1.32 mmol) and 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (175 mg, 0.66 mmol) in isopropanol (10 mL) was added TEA (0.55 mL, 3.96 mmol). The resulting mixture was purged with N 2 , sealed the tube, and heated at 150° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under reduced pressure. To the residue water (15 mL) was added and the resulting mixture was extracted with ethyl acetate (15 mL×2). The combined organic layer was given brine wash, dried over anhydrous sodium sulphate, and concentrated under reduced pressure. The crude obtained subjected to silica gel (230-400) column chromatography using 5-35% ethyl acetate in n-hexane to afford the desired compound as an off-white solid. Yield: 10.2 g (5%); LC_MS Calculated. for C 17 H 19 FN 7 is 340.17, Observed: 340.25; [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.10 (s, 2H), 7.51 (t, J=84 Hz, 1H), 7.15 (q, J=4.4 Hz, 1H), 6.85 (t, J=5.2 Hz, 1H), 3.70 (q, J=6.0 Hz, 1H), 3.05 (t, J=4.8 Hz, 2H), 2.45 (s, 3H), 2.22 (s, 3H), 1.95 (s, 3H); HPLC Purity=95.27%)(Bridge C18 (4.6×150) mm, 5μ; Mobile Phase A: 10 mM ammonium bicarbonate in H 2 O: Mobile Phase B: Acetonitrile; Retention time=10.10 min.

Example 42: 7-amino-2-ethyl-3-methyl-5-((2-(6-methylpyridin-2-yl) ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a mixture of 7-amino-2-ethyl-3-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (0.200 g, 0.716 mmol) XXVIII and 2-(6-methylpyridin-2-yl) ethan-1-amine XIV (0.300 g, 2.20 mmol) in isopropanol (5 mL) was added triethylamine (0.6 mL, 4.0 mmol). The resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. The reaction mixture was cooled to room temperature and the reaction mixture was concentrated under vacuo. To the residue were added ethyl acetate (50 mL) and water (50 mL) and mixed well. The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was purified by combi-flash (230-400) silica gel column chromatography using ethyl acetate in n-hexane and peak eluted with 70% ethyl acetate in n-hexane fraction solvent was concentrated to afford the desired compound as an off-white solid. Yield: 0.088 g (44%), LC_MS Calculated. for is 336.42, Observed: 336.30; [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.07 (bs, 2H), 7.60 (t, J=8.0 Hz, 1H), 7.08 (d, J=8.0 Hz, 2H), 6.91 (t, J=5.2 Hz, 1H), 3.69-3.64 (m, 2H), 2.99 (t, J=6.8 Hz, 2H), 2.67-2.58 (m, 2H), 2.47 (s, 3H), 1.98 (s, 3H), 1.19 (t, J=7.2 Hz, 3H); HPLC Purity=97.67%) (Bridge C18 (4.6×150) mm, 5μ, Mobile phase A: 10 mM ammonium bicarbonate in H 2 O: Mobile Phase B: Acetonitrile; Retention time=10.406 min.

›Abbreviations · 57 of 69

Example 43: 7-amino-2-ethyl-5-((2-(6-(2-hydroxypropan-2-yl) pyridin-2-yl) ethyl) amino)-3-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2-ethyl-3-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXVIII (150 mg, 0.537 mmol) in isopropanol (5 mL) were added triethylamine (0.3 mL, 2.68 mmol) followed by 2-(6-(2-aminoethyl) pyridin-2-yl) propan-2-ol XV (0.145 g, 0.80 mmol). The resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 120 h. The progress of the reaction was monitored by TLC analysis. Then reaction was cooled to room temperature and added water (50 mL). The aqueous layer was extracted with ethyl acetate (50 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuum to afford light brown viscous liquid. The crude material was purified by prep HPLC method using YMC AQUA ODS (250×20) mm, 5.0 microns column; Mobile phase A: 10 mM Ammonium bicarbonate in water. Mobile phase B: ACN:MeOH (1:1) to afford the desired title compound Example 43 as an off-white solid. Yield: 0.014 g (7%); LC_MS Calculated. for C 20 H 25 N 7 O is 379.21, Observed, 380.30; [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.06 (bs, 2H), 7.67 (t, J=7.6 Hz, 1H), 7.46 (d, J=7.6 Hz, 1H), 7.11 (d, J=7.6 Hz, 1H), 6.61 (bs, 1H), 5.16 (s, 1H), 3.72-3.71 (m, 2H), 3.03 (t, J=6.4 Hz, 2H), 2.67-2.59 (m, 2H), 1.98 (s, 3H), 1.43 (s, 6H), 1.19-1.17 (m, 3H); HPLC Purity=97.65%)(Bridge C18 (4.6×150) mm, 5μ; Mobile Phase A: 10 mM ammonium bicarbonate in H 2 O: Mobile Phase B: Acetonitrile; Retention time=10.42 min.

Example 44: 7-amino-5-((2-(6-(hydroxymethyl) pyridin-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Step-1: 2-amino-5-((2-(6-(methoxymethyl) pyridin-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (44a)

A stirred solution of 2-(6-(methoxymethyl) pyridin-2-yl) ethan-1-amine XXI (0.414 g, 2.49 mmol) and 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.300 g, 1.13 mmol) in isopropanol (7 mL) was added triethylamine (0.8 mL, 5.65 mmol) and the resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 120° C. for 48 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to room temperature and the reaction mixture was concentrated under vacuo. To the residue were added ethyl acetate (50 mL) and water (50 mL) and mixed well. The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was purified by combi-flash (230-400) silica gel column chromatography using ethyl acetate in n-hexane and peak eluted with 20% ethyl acetate in n-hexane. Product fractions solvent was concentrated to afford the desired title compound Example 44a as off-white solid. Yield: 0.230 g (58%); LC_MS Calculated. for C 18 H 21 N 7 O is 351.18, Observed, 352.25; [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.11 (bs, 2H), 7.73 (d, J=7.6 Hz, 1H) 7.22 (m, 2H), 6.84 (m, 1H) 4.51 (S, 2H), 3.68-3.67 (m, 2H), 3.36 (s, 3H), 3.02 (t, J=6.8 Hz, 2H), 2.27 (s, 3H), 1.96 (s, 3H), HPLC Purity=98.27%, INT ODS 3V-C18 (4.6×250) mm, 5μ; Mobile Phase A: 0.1% Formic acid in water: Mobile Phase B: Acetonitrile; Retention time=8.70 min.

Step-2: 7-amino-5-((2-(6-(hydroxymethyl) pyridin-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (44)

A stirred solution of 7-amino-5-((2-(6-(methoxymethyl) pyridin-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a] pyrimidine-6-carbonitrile Example 44a (120 mg, 0.341 mmol) in DCM (5 mL) was cool to 0° C. then added BBr 3 (0.03 mL, 0.341 mmol) and the reaction mixture was stirred at 0° C. for 30 min. The progress of the reaction was monitored by TLC. After completion of the reaction, the reaction mixture was quenched with NaHCO 3 solution 2 ml and extracted with DCM (10 ml×3) and dried over anhydrous sodium sulphate and concentrated at low vacuum. Crude solid was washed with 2 ml acetonitrile to get the pure title compound Example 44 as off-white solid. Yield: 0.230 g (58%); LC_MS Calculated for C 17 H 19 N 7 O is 338.4, [M+H] + ; Observed 337.17; [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.05 (bs, 2H), 7.72 (d, J=7.6 Hz, 1H) 7.31 (d, J=7.2 Hz, 1H), 7.15 (d, J=7.6 Hz, 1H) 6.78 (bs, 1H) 5.36 (bs, 1H), 4.58 (S, 2H), 3.68-3.67 (m, 2H), 3.01 (t, J=6.4 Hz, 2H), 2.22 (s, 3H), 1.96 (s, 3H), HPLC Purity=98.19%, XBridge C18 (4.6×150) mm, 5μ, Mobile Phase A: 10 mM ammonium bicarbonate in water: Mobile Phase B: Acetonitrile; Retention time=8.30 min.

Example 45: 7-amino-3-ethyl-5-((2-(6-(hydroxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Step-1: Synthesis of 7-amino-3-ethyl-5-((2-(6-(methoxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrilev (Example 45a)

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (2.0 g, 7.2 mmol) in isopropanol (40 mL) were added TEA (5.9 mL, 43 mmol) and 2-(6-(methoxymethyl) pyridin-2-yl) ethan-1-amine XXI (3.0 g, 18 mmol). The resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to room temperature and the reaction mixture was concentrated under vacuo. To the residue were added ethyl acetate (50 mL) and water (50 mL) and mixed well. The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was purified by combi-flash silica gel (230-400) column chromatography using 10-70% ethyl acetate in n-hexane to afford the desired compound Example 45a as off-white solid. Yield: 1.3 g (50%); LC_MS Calculated for C 19 H 23 N 7 O is 365.20; Observed. 366.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.112 (bs, 2H), 7.751-7.713 (t, J=7.2 Hz, 1H), 7.256-7.185 (m, 2H), 6.854 (bs, 1H), 4.507 (s, 2H), 3.693-3.647 (m, 2H), 3.361 (s, 3H), 3.044-3.010 (t, J=6.8 Hz, 2H), 2.469-2.431 (m, 2H), 2.247 (s, 3H), 1.139-1.101 (t, J=7.6 Hz, 3H).

›Abbreviations · 58 of 69

Step-2:7-amino-3-ethyl-5-((2-(6-(hydroxymethyl) pyridin-2-yl) ethyl)amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

A stirred solution of 7-amino-3-ethyl-5-((2-(6-(methoxymethyl) pyridin-2-yl)ethyl)amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (75 mg, 0.205 mmol) in DCM (5 mL) was cooled to 0° C. and added BBr 3 (0.0 19 mL, 0.205 mmol). The resulting mixture was stirred at 0° C. for 1 h. The progress of the reaction was monitored by TLC analysis. After completion of the reaction, reaction mixture was quenched with sat. NaHCO 3 solution (2 mL) and extracted with DCM (10 mL×3). Combined organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated under vacuo. The crude solid was washed with n-pentane (2 mL) to get the pure compound as an off-white solid. Yield: 30 mg (42%); LCMS Calculated. for C 18 H 21 N 7 O is 351.18, [M+H] + ; Observed, 352.30; [M+H] + ; 1H NMR (400 MHz, DMSO-D 6 ): δ 8.085 (bs, 2H), 7.739-7.700 (t, J=8.0 Hz, 1H) 7.326-7.306 (d, J=8.0 Hz, 1H), 7.149-7.130 (d, J=7.6 Hz, 1H), 6.873-6.846 (t, J=5.2 Hz, 1H), 5.371-5.342 (d, J=5.6 Hz, 1H), 4.588-4.574 (d, J=5.6 Hz, 2H), 3.687-3.639 (m, 2H), 3.030-2.996 (d, J=6.8 Hz, 2H), 2.449-2.434 (m, 2H), 2.248 (s, 3H), 1.175-1.105 (t, J=7.2 Hz, 3H), HPLC Purity=99.09%, Retention time=8.228 min. INT ODS 3V-C18 (4.6×250) mm, 5μ; 0.1% Formic acid in water: Mobile Phase B: Acetonitrile; Flow rate: 1.0 mL/min.

Example 46: 7-amino-3-(cyclopropyl methyl)-5-((2-(6-(hydroxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Step-1: Synthesis of 7-amino-3-(cyclopropyl methyl)-5-((2-(6-(methoxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile (46a)

A stirred solution of 7-amino-3-(cyclopropyl methyl)-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile V (220 mg, 0.720 mmol) in isopropanol (10 mL) were added 2-(6-(methoxymethyl) pyridin-2-yl) ethan-1-amine XXI (299 mg, 1.80 mmol) and TEA (0.703 mL, 5.04 mmol). The resulting mixture was purged with nitrogen for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to room temperature and the reaction mixture was concentrated under vacuo. To the residue were added ethyl acetate (50 mL) and water (50 mL) and mixed well. The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuum to afford light brown viscous liquid. The crude compound was purified by combi-flash (230-400) silica gel column chromatography using ethyl acetate in DCM and peak eluted with 25% ethyl acetate in DCM, fraction solvent was concentrated to afford the desired compound Example 46a as off-white solid. Yield: 0.160 g (57%); LC_MS Calculated. for C 21 H 25 N 7 O is 391.47 Observed. 392.30 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.12 (bs, 2H), 7.73 (t, J=7.2 Hz, 1H), 7.25-7.17 (m, 2H), 6.86 (bs, 1H), 4.50 (s, 2H), 3.68-3.65 (m, 2H), 3.36 (s, 3H), 3.04-3.00 (t, J=6.4 Hz, 2H), 2.38-2.33 (m, 2H), 2.26 (s, 3H), 0.95-0.94 (m, 1H), 0.36-0.34 (m, 2H), 0.17-0.16 (m, 2H). HPLC Purity=96.56%, INT ODS 3V-C18 (4.6×250) mm, 5μ; Mobile Phase A: 0.1% Formic acid in water: Mobile Phase B: Acetonitrile; Retention time=9.49 min.

Step-2: 7-amino-3-(cyclopropyl methyl)-5-((2-(6-(hydroxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

A stirred solution of 7-amino-3-(cyclopropyl methyl)-5-((2-(6-(methoxymethyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (70 mg, 0.18 mmol) in DCM (5 mL) was added BBr 3 (0.017 mL, 0.18 mmol) at 0° C. The resulting mixture was stirred for 30 min at 0° C. The progress of the reaction was monitored by TLC analysis. After completion of the reaction mixture was quenched with NaHCO 3 solution (2 ml) and extracted with dichloromethane (10 ml×3) and dried over sodium sulphate and concentrated at low vacuum. Crude was purified by prep-HPLC. Method: Xbridge C18, 19×250 mm, 5 mic. 10 mm ammonium bicarbonate in water and acetonitrile. Prep fractions was evaporated to get pure title compound Example 46 as off-white solid. Yield: 6.8 mg (10%). LCMS Calculated. for C 20 H 23 N 7 O is 377.20 [M+H] + ; Observed. 378.3 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.11 (bs, 2H), 7.73-7.96 (m, 1H), 7.31 (d, J=7.6 Hz, 1H), 7.13 (d, J=7.6 Hz, 1H), 6.87 (bs, 1H), 5.35 (t, J=5.6 Hz, 1H), 4.58-4.57 (m, 2H), 3.66-3.64 (m, 2H), 3.00 (t, J=6.8 Hz, 3H), 2.39-2.32 (m, 2H), 2.26 (s, 3H), 0.85-0.83 (m, 1H), 0.36-0.34 (m, 2H), 0.17-0.16 (m, 2H). HPLC Purity=92.88%, INT ODS 3V-C18 (4.6×250) mm, 5μ; Mobile Phase A: 0.1% Formic acid in water: Mobile Phase B: Acetonitrile; Retention time=9.58 min.

Example 47: 7-amino-2-cyclopropyl-5-((2-(6-(2-hydroxypropan-2-yl) pyridin-2-yl) ethyl)amino)-3-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of 7-amino-2-cyclopropyl-3-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX1X (0.13 g, 0.45 mmol) in IPA (4 mL) at rt under N 2 atmosphere were added 2-(6-(2-aminoethyl) pyridin-2-yl) propan-2-ol XV (0.24 g, 1.3 mmol) and triethylamine (0.31 mL, 2.2 mmol) and the seal tube was heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After completion of the reaction, the reaction mixture was cooled to rt, and 20 mL water was added. The resulting mixture was extracted with ethyl acetate (20 mL×2). The combined organic layers were washed with brine, dried over anhydrous Na 2 SO 4 and concentrated under vacuo to afford light brown semi solid. The compound was purified by reverse phase HPLC using Mobile phase A: 10 mM ammonium bicarbonate in water: Mobile phase B: acetonitrile (column: YMC pack ODS-Aq; 250×20 mm; 50 um) and the peak eluted at retention time 17 min with 50% acetonitrile and flow rate 17 mL/min was concentrated to afford the desired compound Example 47 as off-white solid. Yield: 3.4 mg (2%), LC_MS Calculated. for C 21 H 26 N 7 O is 392.49, Observed, 392.30; [M+H] HPLC Purity=97.04%, Column: INT ODS 3V-C18 (4.6×250) mm, Mobile phase A: 0.1% TFA in water Mobile phase B: Acetonitrile

›Abbreviations · 59 of 69

Example 48: 7-amino-2-(difluoromethyl)-3-ethyl-5-((2-(1-(1-hydroxy-2-methylpropan-2-yl)-1H-pyrazol-3-yl) ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2-(difluoro methyl)-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile XXX (0.3 g, 0.951 mmol) and 2-(3-(2-aminoethyl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol XVIII (0.523 g, 2.85 mmol) in IPA (5 mL) was added TEA (0.8 mL, 5.71 mmol) and the reaction mixture was heated at 150° C. for 24 h. The progress of the reaction was monitored by TLC. After completion, after completion, the reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (20 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude material was purified by column chromatography by eluting with 50% ethyl acetate in hexane to yield the title compound as off-white solid. Yield: 0.098 g (25%); LC_MS Calculated. for C 19 H 24 F 2 N 8 O is 418.20, Observed. 419.30; [M+1-1] + . 1H NMR (400 MHz, dmso-d6): δ 8.42 (s, 2H), 7.64 (d, J=4 Hz, 1H), 7.04-6.90 (m, 2H), 6.05 (d, J=2 Hz, 1H), 4.88 (s, 1H), 3.61-3.32 (m, 4H), 2.83 (t, J=4 Hz, 2H), 2.62-2.50 (m, 2H), 1.43 (s, 6H), 1.16 (t, J=4 Hz, 3H). HPLC Purity=99.09%, Column: XBridge C18 (4.6×150) mm, 5μ Mobile phase A: 10 mM ammonium bicarbonate in H 2 O Mobile phase B: acetonitrile; Retention time=10.250 min.

Example 49: 7-amino-5-((2-(6-(1-(hydroxymethyl) cyclopropyl) pyridin-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Step-1: 7-amino-5-((2-(6-(1-(methoxymethyl) cyclopropyl) pyridin-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (49a)

To a solution of 2-(6-(1-(methoxymethyl) cyclopropyl) pyridin-2-yl) ethan-1-amine (0.53 g, 2.6 mmol) XXXI and 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.34 g, 1.3 mmol) in isopropanol (10 mL) was added triethylamine (0.54 mL, 3.8 mmol) and the resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was cooled to room temperature and concentrated under vacuo. To this, added ethyl acetate (20 mL) and water (20 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was subjected to silica gel (230-400; packed 40 g) column chromatography using ethyl acetate (0-25%) in n-hexane. The peak eluted with 22% ethyl acetate in hexane was concentrated to afford the desired compound Example 49a as off-white solid. Yield: 100 mg (20%); LC_MS Calculated. for C 21 H 25 N 7 O is 391.48, Observed 392.25; [M+H] + ; HPLC Purity=95.12%, INT_9010_AA_22.1 cm; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.08 (S, 2H), 7.58 (d, J=8 Hz 1H), 7.24 (d, J=8.0 Hz, 1H), 7.02 (d, J=8 Hz, 1H), 6.57 (S, 1H), 3.68 (t, J=8 Hz, 4H), 3.30 (d, J=20 Hz, 3H), 2.96 (t, J=8 Hz, 2H), 2.23 (S, 3H), 1.97 (S, 3H), 1.19 (S, 2H), 0.87 (d, J=4 Hz, 2H).

Step-2: 7-amino-5-((2-(6-(1-(hydroxymethyl) cyclopropyl) pyridin-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of 7-amino-5-((2-(6-(1-(methoxymethyl)cyclopropyl)pyridin-2-yl)ethyl)amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile Example 49a (70 mg, 0.18 mmol) in DCM (7 mL) at 0° C. was added BBr 3 (0.02 mL, 0.18 mmol) drop wise and stirred for 30 min. The reaction mixture was monitored by TLC analysis in which both bromo analogue and desired product was observed. Then to the resulting mixture, sodium formate (0.007 mL, 0.18 mmol) and catalytic amount of TBAB was added and stirred for 48 h. The reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was quenched with saturated NH 4 Cl solution. To this, water (2 mL) was added and extracted with DCM (2 mL×2). The combined organic layer was washed with brine and concentrated under reduced pressure to afford a light brown viscous liquid. The crude compound was purified by reverse phase HPLC; YMC AQUA ODS (250×20) mm. 5.0 microns. Mobile phase A: 10 mm Ammonium bicarbonate in H 2 O; Mobile phase B: ACN:MeOH (1:1) to afford the desired title compound Example 49 as a light brown solid. Yield: 67 mg (43%); LCMS Calculated. for C 20 H 23 N 7 O is 377.47, Observed, 378.25; [M+H] + ; HPLC Purity=98.27%, Column: Symmetry-C18 (4.6*75) mm, 3.5μ Mobile phase A: 10 mM ammonium bicarbonate in water. Mobile phase B: ACN. 1 H NMR (400 MHz, dmso-d6): δ 8.08 (S, 2H), 7.59 (t, j=8 Hz 1H), 7.29 (t, J=8.0 Hz, 1H), 7.02 (d, j=8 Hz, 1H), 6.57 (t, j=8 Hz 1H), 4.72 (t, J=4 Hz, 1H), 3.75-3.66 (m, 4H), 2.96 (t, J=8 Hz, 2H), 2.23 (S, 3H), 1.97 (S, 3H), 1.19 (S, 2H), 0.87 (d, J=4 Hz, 2H).

Step-1: 7-amino-3-ethyl-5-((2-(6-(1-(methoxymethyl) cyclopropyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (50a)

To a solution of 2-(6-(1-(methoxymethyl) cyclopropyl) pyridin-2-yl) ethan-1-amine XXXI (0.24 g, 1.1 mmol) and 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.16 g, 0.57 mmol) in isopropanol (10 mL) was added triethylamine (0.24 mL, 1.7 mmol) and the resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. the progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was cooled to room temperature and concentrated under vacuo. Added ethyl acetate (20 mL) and water (20 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was subjected to silica gel (230-400; packed 40 g) column chromatography using ethyl acetate (0-25%) in n-hexane. The peak eluted with 22% ethyl acetate in n-hexane was concentrated to afford the desired compound Example 50a as an off-white solid. Yield: 0.095 g (41%). LCMS Calculated. for C 22 H 27 N 7 O is 405.51; Observed. 406.35 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.08 (s, 2H), 7.59 (t, J=8 Hz, 1H), 7.24 (d, J=8.0 Hz, 1H), 7.01 (d, J=8 Hz, 1H), 6.60 (t, J=4 Hz 1H), 3.65 (t, J=12 Hz 4H), 3.30 (d, J=16 Hz, 3H), 2.96 (t, J=8 Hz, 2H), 2.50-2.43 (m, 2H), 2.25 (s, 3H), 1.23-1.19 (m, 2H), 1.15 (t, J=8 Hz 3H), 0.89-0.86 (m, 2H); HPLC Purity=98.45%, Column: XBridge C18 (4.6×150) mm, 5 μMobile phase A: 10 mM ammonium bicarbonate in H 2 O. Mobile phase B: acetonitrile

›Abbreviations · 60 of 69

Step-2: 7-amino-3-ethyl-5-((2-(6-(1-(hydroxymethyl) cyclopropyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a solution of 7-amino-3-ethyl-5-((2-(6-(1-(methoxymethyl) cyclopropyl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile Example 50a (70 mg, 0.17 mmol) in DCM (7 mL) at 0° C. was added BBr 3 (0.02 mL, 0.17 mmol) in dropwise and stirred for 30 min. The reaction mixture was monitored by TLC analysis in which both bromo analogue and desired product was observed. Then added sodium formate (0.007 mL, 0.17 mmol) and catalytic amount of TBAB, stirred for 48 h. The reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was quenched with saturated NH 4 Cl solution. Added water (2 mL) and extracted with DCM (2 mL×2). The combined organic layer was washed with brine and concentrated under reduced pressure to afford a light brown viscous liquid. The crude compound was purified by Reverse phase HPLC: YMC AQUA ODS (250×20) mm, 5.0 microns; Mobile phase A: 10 mm Ammonium bicarbonate in H 2 O; Mobile phase B: ACN:MeOH (1:1) to afford the desired title compound Example 50 as an off-white solid. Yield: 0.015 g (22%). LCMS Calculated. for C 21 H 25 N 7 O is 391.47; Observed. 392.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.08 (s, 2H), 7.59 (t, J=8.0 Hz, 1H), 7.24 (d, J=8.0 Hz, 1H), 7.01 (d, J=8.0 Hz, 1H), 6.60 (t, J=4.0 Hz 1H), 4.71 (d, J=4.0 Hz, 1H) 3.74-3.65 (m, 4H), 2.96 (t, J=8.0 Hz, 2H), 2.50-2.43 (m, 2H), 2.25 (s, 3H), 1.23-1.19 (m, 2H), 1.15 (t, J=8.0 Hz 3H), 0.89-0.86 (m, 2H); HPLC Purity=98.78%, Column: Symmetry-C18 (4.6×75) mm, 3.5μ; Mobile phase A: 10 mM ammonium acetate in H 2 O Mobile phase B: Acetonitrile.

Example 51: 7-amino-5-((2-(6-(2-(hydroxymethyl) cyclopropyl) pyridin-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of (2-(6-(2-aminoethyl) pyridin-2-yl) cyclopropyl) methanol XXV (0.36 g, 1.9 mmol) and 7-amino-2,3-dimethyl-5-(methylsulfonyl)pyrazolo[1,5-a]pyrimidine-6-carbonitrile I (0.25 g, 0.94 mmol) in IPA (10 mL) was added triethylamine (0.39 mL, 2.8 mmol) and purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was cooled to rt and concentrated under vacuo. Added water (20 mL) and extracted with ethyl acetate (20 mL×2). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was purified by silica gel (230-400 mesh) column chromatography using ethyl acetate in n-hexane (0-80%) to afford the desired title compound Example 51 as an off-white solid. Yield: (30 mg, 8.4%); LC_MS Calculated. for C 20 H 23 N 7 O is 377.45; Observed. 378.25 [M+H] + . HPLC Purity=99.36%, Column: YMC-Pack ODS-AQ (4.6×150) mm, 5μ Mobile phase A: 10 mM ammonium acetate in H 2 O; Mobile phase B: ACN; Rt=11.620 min. 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.09 (s, 2H), 7.53 (t, J=8 Hz, 1H), 7.06-6.98 (m, 2H), 6.65 (t, J=4 Hz, 1H), 4.56 (t, J=6.8 Hz, 1H), 3.68-3.35 (m, 4H), 2.95 (t, J=8 Hz, 2H), 2.23 (s, 3H), 1.95 (t, J=4 Hz, 4H), 1.59-1.54 (m, 1H), 1.10-1.06 (m, 1H), 1.06-0.85 (m, 1H).

Example 52: 7-amino-3-ethyl-5-((2-(6-(1-hydroxy-2-methylpropan-2-yl) pyridin-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a solution of 2-(6-(2-aminoethyl) pyridin-2-yl)-2-methylpropan-1-ol XXXII (0.2 g, 1 mmol) and 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile II (0.2 g, 0.7 mmol) in isopropanol (10 mL) was added triethylamine (0.4 mL, 3 mmol) and the resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to RT and the reaction mixture was concentrated under vacuo. To the residue was added ethyl acetate (20 mL) and water (20 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was purified by reverse phase HPLC, Column: Xbridge Prep C18 (250×19) mm, 5.0μ; Mobile phase A: 10 mm Ammonium bi carbonate in water; Mobile phase B: Acetonitrile to afford the desired title compound Example 52 as an off-white solid. Yield: 32 mg (10%); LC_MS Calculated. for C 21 H 28 N 7 O is 394.24; Observed. 394.30 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.09 (bs, 2H), 7.63 (t, J=7.6 Hz, 1H), 7.22 (d, J=8.0 Hz, 1H), 7.07 (d, J=7.2 Hz, 1H), 6.65 (bs, 1H), 4.64 (t, J=5.6 Hz, 1H), 3.73-3.68 (m, 2H), 3.55 (d, J=5.6 Hz, 2H), 3.02 (t, J=6.8 Hz, 2H), 2.46-2.41 (m, 2H), 2.25 (s, 3H), 1.26 (s, 6H), 1.13 (t, J=7.2 Hz, 3H); HPLC Purity=98.27%, YMC-Pack ODS-AQ(4.6×150) mm, 5μ, Mobile phase A: 10 mM ammonium acetate in water Mobile phase B: Acetonitrile.

Example 53: 7-amino-5-((2-(6-(1-hydroxy-2-methylpropan-2-yl) pyridine-2-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of 2-(6-(2-aminoethyl) pyridin-2-yl)-2-methylpropan-1-ol (0.2 g, 1 mmol) and 7-amino-2,3-dimethyl-5-(methylsulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile (0.2 g, 0.8 mmol) in isopropanol (10 mL) was added triethylamine (0.4 mL, 3 mmol) and the resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to RT and the reaction mass was concentrated under vacuo. To the residue were added ethyl acetate (20 mL) and water (20 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was purified by reverse phase HPLC, Column: Xbridge Prep C18 (250×19) mm, 5.0μ; Mobile phase A: 10 mm Ammonium bicarbonate in water; Mobile phase B: Acetonitrile to afford the desired compound Example 53 as off-white solid. Yield: 32 mg (10%); LC_MS Calculated. for C 20 H 26 N 7 O is 380.22; Observed. 380.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.09 (bs, 2H), 7.63 (t, J=7.6 Hz, 1H), 7.22 (d, J=8.0 Hz, 1H), 7.09 (d, J=7.6 Hz, 1H), 6.62 (bs, 1H), 4.65 (t, J=5.6 Hz, 1H), 3.74-3.69 (m, 2H), 3.55 (d, J=5.6 Hz, 2H), 3.02 (t, J=7.2 Hz, 2H), 2.23 (s, 3H), 1.97 (s, 3H), 1.24 (s, 6H); HPLC Purity=98.27%, YMC-Pack ODS-AQ(4.6×150) mm, 5μ, Mobile phase A: 10 mM ammonium acetate in H 2 O: Acetonitrile.

›Abbreviations · 61 of 69

Example 54: 7-amino-3-ethyl-5-((2-(1-(2-hydroxyethyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Step-1: 7-amino-3-ethyl-5-((2-(1-(2-methoxyethyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile (Example 54a)

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile II (0.700 g, 2.51 mmol) and 2-(1-(2-methoxyethyl)-1H-pyrazol-3-yl)ethan-1-amine XXIII (0.85 g, 5.02 mmol) in isopropanol (2.5 mL) in a sealed tube was added triethylamine (2.10 mL, 15.0 mmol) and the reaction mixture was stirred at 150° C. for 24 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (20 mL) and extracted with ethyl acetate (20 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude material was purified by silica gel (100-200) column chromatography by eluting with 50% ethyl acetate in n-hexane to yield the title compound Example 54a as an off-white solid. Yield: 0.3 g (32.5%); LC_MS Calculate. for C 18 H 25 N 8 O is 369.22; Observed. 369.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.10 (bs, 2H), 7.58 (d, J=2.0 Hz, 1H), 6.66 (t, J=5.2 Hz, 1H), 6.06 (d, J=2.0 Hz, 1H), 4.18 (q, J=5.2 Hz, 2H), 3.66 (t, J=5.2 Hz, 2H), 3.59-3.55 (m, 2H), 2.21 (s, 3H), 2.82 (t, J=7.2 Hz, 2H), 2.47-2.43 (m, 2H), 2.25 (s, 3H), 1.12 (t, J=7.6 Hz, 3H); HPLC Purity=99.78%, YMC-Pack ODS-AQ (4.6×150) mm, 5μ, Mobile phase A: 10 mM ammonium acetate in water. Mobile phase B: Acetonitrile.

Step-2: 7-amino-3-ethyl-5-((2-(1-(2-hydroxyethyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

A stirred solution of 7-amino-3-ethyl-5-((2-(1-(2-methoxyethyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile Example 54a (0.3 g, 0.8 mmol) in DCM (50 ml) was cooled to 0° C., and BBr 3 (0.09 mL, 1 mmol) was added slowly. After completion, the reaction mixture was quenched with methanol (0.5 mL) and diluted with water (20 mL). The resulting mixture was extracted with DCM (20 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude material was purified by silica gel (100-200) column chromatography by eluting with 1-5% methanol in DCM to afford the desired title compound Example 54 as off-white solid. Yield: 50 mg (33%); LC_MS Calculated for C 17 H 23 N 8 O is 355.20; Observed. 355.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.29 (bs, 2H), 7.65 (s, 1H), 6.89 (bs, 1H), 6.12 (s, 1H), 4.09 (t, J=5.6 Hz, 2H), 3.71 (t, J=5.6 Hz, 2H), 3.62 (t, J=7.2 Hz, 2H), 2.86 (t, J=7.2 Hz, 2H), 2.55-2.50 (m, 3H), 2.26 (s, 3H), 1.11 (t, J=7.6 Hz, 3H); HPLC Purity=99.78%, YMC-Pack ODS-AQ (4.6×150) mm, 5μ, Mobile phase A: 10 mM ammonium acetate in water. Mobile phase B: Acetonitrile.

Example 55: 7-amino-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

In a seal tube was charged with 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (2.1 g, 7.9 mmol), (1-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopropyl) methanol XXIV (2 g, 11 mmol) and isopropanol (60 mL). The resulting mixture was purged with N 2 gas for 5 min and TEA (6.6 mL, 47 mmol) was added. The seal tube was stirred at 140° C. for 3 days. The progress of reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated to remove the solvent. The residue was dissolved in water (40 mL) and extracted with ethyl acetate (50 mL×3). The combined organic layer was washed with brine and dried over anhydrous sodium sulphate. The crude material obtained upon removal of the solvent was purified by flash chromatography (combi-flash) using 50-100% ethyl acetate in n-hexane to afford the desired compound as an off-white solid. Yield: 1.0 g (34.4%); LC_MS Calculated. for C 18 H 23 N 8 O is 367.20; Observed. 367.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.12 (bs, 2H), 7.62 (d, J=2.0 Hz, 1H), 6.58 (t, J=5.6 Hz, 1H), 6.05 (d, J=2.0 Hz, 1H), 4.89 (d, J=5.6 Hz, 1H), 3.61-3.56 (m, 4H), 2.81 (t, J=7.2 Hz, 2H), 2.50 (s, 3H), 1.97 (s, 3H), 1.10-1.07 (m, 2H), 0.98-0.95 (m, 2H); HPLC Purity=99.01%.

Example 56: 7-amino-2-(difluoro methyl)-3-ethyl-5-((2-(6-(hydroxymethyl) pyridine-2-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile

Step-1: 7-amino-2-(difluoro methyl)-3-ethyl-5-((2-(6-(methoxymethyl) pyridin-2-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (Example 56a)

To a stirred solution of 7-amino-2-(difluoro methyl)-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXX (200 mg, 0.634 mmol) in isopropanol (6 mL) were added TEA (0.53 mL, 3.81 mmol) and 2-(6-(methoxymethyl) pyridin-2-yl) ethan-1-amine XXI (264 mg, 1.59 mmol). The resulting mixture was purged with N 2 gas for 5 min and the seal tube was heated at 160° C. with vigorous stirring for 48 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under reduced pressure. The crude was subjected to silica gel (230-400) combi-flash column chromatography using 0-25% ethyl acetate/n-hexane to afford the desired compound Example 56a as an off-white solid. Yield: 140 mg (55%); LC_MS Calculated. for C 19 H 22 F 2 N 7 O is 402.19; Observed. 402.20 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.43 (bs, 2H), 7.73 (t, J=7.6 Hz, 1H), 7.26-7.21 (m, 2H), 7.13-6.91 (m, 2H), 4.50 (s, 2H), 3.69 (bs, 1H), 3.36 (s, 3H), 3.04 (t, J=6.4 Hz, 2H), 2.67-2.61 (m, 3H), 1.16 (t, J=7.6 Hz, 3H).

Step-2: 7-amino-2-(difluoro methyl)-3-ethyl-5-((2-(6-(hydroxymethyl) pyridin-2-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile

A stirred solution of 7-amino-2-(difluoro methyl)-3-ethyl-5((2-(6-(methoxymethyl) pyridin-2-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile Example 56a (140 mg, 0.349 mmol) in dry DCM (5 mL) was cooled to 0° C. under N 2 atmosphere and BBr 3 (49.5 μL, 0.523 mmol) in dry DCM (0.5 mL) was slowly added. The reaction mixture was stirred at 0° C. for 2 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction cooled to 0° C. and was quenched with MeOH (0.5 mL). The reaction mixture was concentrated under reduced pressure and the crude was stirred in sat. NaHCO 3 solution for 30 min. The solid was collected by filtration and was purified by silica gel (230-400 mesh) column chromatography using 30-80% ethyl acetate in n-hexane to afford the desired title compound Example 56 as off-white solid. Yield: 72.2 mg (53%); LC_MS Calculated. for C 17 H 22 N 8 O is 355.20; Observed. 355.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.14 (bs, 2H), 7.65 (d, J=1.6 Hz, 1H), 6.79 (bs, 1H), 6.16 (t, J=2.0 Hz, 1H), 4.92 (d, J=5.6 Hz, 1H), 4.38 (d, J=5.2 Hz, 2H), 3.55 (d, J=5.6 Hz, 2H), 2.23 (s, 3H), 1.97 (s, 3H), 1.44 (s, 6H). HPLC purity=98.22%; XBridge C18 (4.6*150) mm, 5μ; Mobile Phase A: 10 mM ammonium bicarbonate in water; Mobile Phase B: Acetonitrile.

›Abbreviations · 62 of 69

Example 57: 7-amino-5-(((1-(1-hydroxy-2-methylpropan-2-yl)-1H-pyrazol-3-yl) methyl) amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (0.15 g, 0.57 mmol) and 2-(3-(aminomethyl)-1H-pyrazol-1-yl)-2-methylpropan-1-ol (0.12 g, 0.71 mmol) in 2-propanol XXXIII (5 mL), was added triethylamine (0.47 mL, 3.4 mmol) and the reaction mixture was heated at 120° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (100 ml) and extracted with ethyl acetate (100 mL×3). The organic layer was dried with Na 2 SO 4 , filtered, and evaporated under reduced pressure. The crude was purified by flash silica gel (230-400 mesh) using 0-2% methanol in DCM to afford the desired title compound Example 57 as an off-white solid. Yield: 45.0 mg (20%); LC_MS Calculated. for C 17 H 22 N 8 O is 355.20; Observed. 355.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.14 (bs, 2H), 7.65 (d, J=1.6 Hz, 1H), 6.79 (bs, 1H), 6.16 (t, J=2.0 Hz, 1H), 4.92 (d, J=5.6 Hz, 1H), 4.38 (d, J=5.2 Hz, 2H), 3.55 (d, J=5.6 Hz, 2H), 2.23 (s, 3H), 1.97 (s, 3H), 1.44 (s, 6H); HPLC purity=98.22%; XBridge C18 (4.6×150) mm, 5μ; Mobile Phase A: 10 mM ammonium bicarbonate in H 2 O; Mobile Phase B: Acetonitrile.

Example 58: 7-amino-2-(difluoro methyl)-3-ethyl-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

A seal tube was charged with 7-amino-2-(difluoro methyl)-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (146 mg, 0.463 mmol), (1-(3-(2-aminoeth yl)-1H-pyrazol-1-yl) cyclopropyl)methanol (150 mg, 0.828 mmol) and 2-propanol (6 mL). The resulting mixture was purged with N 2 gas for 5 min and TEA (0.387 mL, 2.78 mmol) was added. The reaction was stirred at 140° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture concentrated, and the residue was dissolved in water (10 mL). The resulting mixture was extracted with ethyl acetate (15 mL×2). The combined organic layer was washed with brine and dried over anhydrous sodium sulphate. The solvent was removed, and the crude material was purified by flash chromatography (combi-flash) using 20-80% ethyl acetate in n-hexane to afford the desired compound as an off-white solid. Yield: 18 mg (9.3%); LC_MS Calculated. for C 19 H 23 F 2 N 8 O is 417.20; Observed. 417.20 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.45 (bs, 2H), 7.62 (s, 1H), 7.12-6.87 (m, 2H), 6.08 (s, 1H), 4.96 (bs, 1H), 3.61-3.58 (m, 4H), 2.84 (bs, 2H), 2.71-2.60 (m, 2H), 1.23-1.16 (m, 3H), 1.14-0.98 (m, 4H); HPLC purity=95.15%;)(Bridge C18 (4.6×150) mm, 5μ; Mobile Phase A: 10 mM NH 4 HCO 3 in water; Mobile Phase B: Acetonitrile; Retention time: 11.678 min.

Example 59: 7-amino-2-(difluoro methyl)-3-ethyl-5-((2-(6-(2-(hydroxymethyl) cyclopropyl)pyridin-2-yl)ethyl)amino)pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of (2-(6-(2-aminoethyl) pyridin-2-yl) cyclopropyl) methanol XXV (0.3 g, 2 mmol) and 7-amino-2-(difluoro methyl)-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXX (0.2 g, 0.8 mmol) in isopropanol (10 mL) was added triethylamine (0.7 mL, 5 mmol) and the resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to RT and the reaction mixture was concentrated under vacuo. To the residue was added ethyl acetate (20 mL) and water (20 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude compound was purified by reverse phase prep-HPLC using YMC AQUA ODS (250×20) mm. 5μ; Mobile phase A: 10 mm Ammonium bicarbonate in H 2 O, Mobile phase B: ACN:MeOH (1:1). Yield: (50 mg, 4%). LC_MS Calculated. for C 12 H 24 F 2 N 7 O is 428.20; Observed. 428.20 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.42 (bs, 2H), 7.53 (t, J=7.6 Hz, 1H), 7.18-6.91 (m, 4H), 4.57 (t, J=6.0 Hz, 1H), 3.66 (bs, 2H), 3.68-3.36 (m, 2H), 2.97 (t, J=6.8 Hz, 2H), 2.68-2.55 (m, 2H), 1.96 (bs, 1H), 1.58 (bs, 1H), 1.19 (t, J=7.2 Hz, 2H), 1.09 (bs, 1H), 1.07 (bs); HPLC Purity=96.58%, XB_9010ABC Rt=10.262 min; Chiral HPLC (two peaks); Chiral Pak IG (250×4.6) mm, 5μ; Mobile phase: A: 0.1% DEA in Hex, B: EtOH (90:10); P1=51.29%, Rt=19.022 min; P2=48.71%, Rt=21.731 min.

Example 60: 7-amino-3-ethyl-5-((2-(6-(2-(hydroxymethyl) cyclopropyl) 284 yridine-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of (2-(6-(2-aminoethyl) pyridin-2-yl) cyclopropyl) methanol XXV (344 mg, 1.79 mmol) and 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (250 mg, 0.89 mmol) in isopropanol (10 mL) was added triethylamine (0.750 mL, 5.37 mmol) and the resulting mixture was purged with N 2 for 10 min. The seal tube was closed tightly and heated at 120° C. for 48 h. The progress of the reaction was monitored by TLC analysis. The reaction was cooled to RT and the reaction mixture was concentrated under vacuo. To the residue was added ethyl acetate (30 mL) and water (20 mL). The organic layer was separated, washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuo to afford light brown viscous liquid. The crude was purified by combi flash (230-400 mesh) column chromatography by using 0-20% ethyl acetate in n-hexane to afford the desired title compound Example 60 as an off-white solid. Yield: (45 mg, 20%). LC_MS Calculated. for C 21 H 26 N 7 O is 392.22; Observed. 392.25 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.09 (bs, 2H), 7.53 (t, J=7.6 Hz, 1H), 7.05 (d, J=7.6 Hz, 1H), 6.98 (d, J=7.6 Hz, 1H), 6.68 (bs, 1H), 4.56 (t, J=5.6 Hz, 1H), 3.67-3.62 (m, 2H), 3.51-3.47 (m, 1H), 3.39-3.32 (m, 1H), 2.95 (t, J=7.2 Hz, 2H), 2.48-2.40 (m, 2H), 2.25 (s, 3H), 1.98-1.94 (m, 1H), 1.59-1.56 (m, 1H), 1.20-1.06 (m, 4H), 0.89-0.85 (m, 1H); HPLC Purity=96.79%, Rt=10.262 min; Chiral HPLC (two peaks); Chiral Pak IG (250×4.6) mm, 5μ; Mobile phase: A: 0.1% DEA In Hex, B: EtOH (90:10); P1=49.98%, Rt=26.408 min; P2=50.02%, Rt=32.475 min.

›Abbreviations · 63 of 69

Example 61: 7-amino-3-chloro-5-((2-(4-(1-(hydroxymethyl) cyclopropyl) thiazol-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX (0.15 g, 0.53 mmol) in isopropanol (5 mL) were added (1-(2-(2-aminoethyl) thiazol-4-yl)cyclopropyl)methanol XXXIV (0.31 g, 1.6 mmol) and TEA (0.37 mL, 2.6 mmol). The reaction mixture was stirred at 150° C. for 24 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated and to the residue was added water (15 mL). The resulting mixture was extracted with ethyl acetate (20 mL×2) and the combined organic layer was dried over anhydrous sodium sulphate. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using (10-60%) ethyl acetate in n-hexane to afford the desired title compound Example 61 as a light brown solid. Yield: 11.3 mg (5%); LC_MS Calculated. for C 17 H 18 ClN 7 OS is 403.10; Observed. 404.15 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.43 (bs, 2H), 7.16 (s, 1H), 7.10 (t, J=5.2 Hz, 1H), 4.69 (t, J=5.6 Hz, 1H), 3.71-3.65 (m, 4H), 3.22 (t, J=6.8 Hz, 2H), 2.28 (s, 3H), 0.99-0.96 (m, 2H), 0.84-0.81 (m, 2H). HPLC Purity=99.01%; INT ODS 3V-C18 (4.6×250) mm, 5μ; Mobile phase A: 0.1% Formic acid in water; Mobile phase B: Acetonitrile; Rt=11.614 min.

Example 62: 7-amino-3-ethyl-5-(2-(4-fluoro-1-(1-hydroxy-2-methylpropan-2-yl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (250 mg, 0.895 mmol) and 2-(3-(2-aminoethyl)-4-fluoro-1H-pyrazol-1-yl)-2-methylpropan-1-ol. XXXV (350 mg, 1.74 mmol) in 2-propanol (10 mL) was added triethylamine (0.748 mL, 5.37 mmol). The resulting mixture was heated at 120° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to room temperature and crude was diluted with ethyl acetate (20 mL) and water (20 mL) and mixed well. The combined organic layer was washed with brine, dried over anhydrous sodium sulphate, and concentrated under vacuum to afford light brown viscous liquid. The crude compound was purified by combi-flash silica (230-400) column chromatography by eluting with 10-50% ethyl acetate in n-hexane to afford the desired compound Example 62 as an off-white solid. Yield: 0.11 g (30%). LC_MS Calculated. for C 19 H 25 FN 8 O is 400.21; Observed. 401.25 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.10 (bs, 2H), 7.79 (d J=4.8 Hz, 1H), 6.65 (bs, 1H), 4.91-4.90 (m, 1H), 3.58-3.55 (m, 2H), 3.51-3.49 (m, 2H), 2.84-2.80 (m, 2H), 2.46-2.42 (m, 2H), 2.25 (m, 3H), 1.39 (s, 6H), 1.11 (t, J=7.60 Hz, 3H); HPLC Purity=99.09%; Rt=10.08 min;

Example 63: 7-amino-3-ethyl-5-((2-(1-((1-(hydroxymethyl) cyclopropyl)methyl)-1H-pyrazol-3-yl)ethyl)amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Step-1: 7-Amino-5-((2-(1-((1-(((tert-butyl dimethyl silyl) oxy) methyl) cyclopropyl) methyl)-1H-pyrazol-3-yl) ethyl) amino)-3-ethyl-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile (Example 63a)

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (250 mg, 0.895 mmol) in 2-propanol (20 mL) was added 2-(1-((1-(((tert-butyl dimethyl silyl) oxy) methyl)cyclopropyl)methyl)-1H-pyrazol-3-yl)ethan-1-amine XXXVII (416 mg, 1.34 mmol) and triethylamine (624 μL, 4.48 mmol) under nitrogen. The resulting mixture was stirred at 130° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to room temperature and diluted with water (30 mL) then extracted with ethyl acetate (20 mL×3). The combined organic layer was washed with 25 mL of brine and passed through anhydrous sodium sulphate then concentrated under reduced pressure. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using 0-5% MeOH in DCM to afford the desired compound Example 63a as a light-yellow solid. Yield: 0.15 g (33%). LC-MS Calculated. for C 26 H 40 N 8 OSi is 508.30; Observed. 509.35 [M+H] + .

Step-2: 7-amino-3-ethyl-5-((2-(1-((1-(hydroxymethyl) cyclopropyl) methyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-5-((2-(1-((1-(((tert-butyldimethyl silyl)oxy)methyl)cyclopropyl)methyl)-1H-pyrazol-3-yl)ethyl) amino)-3-ethyl-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile Example 63a (0.150 g, 0.295 mmol) in THF (5 mL) was added TBAF (154 mg, 1.0 M in THF, 0.590 mmol) and the reaction mixture was stirred at room temperature for 4 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (50 mL) and extracted with ethyl DCM (30 mL×3). The combined organic layer was dried over anhydrous sodium sulphate and concentrated under vacuo to afford a desired crude compound. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using 0-6% MeOH in DCM to afford the desired compound Example 63 as an Off-white solid. Yield: 66 mg (57%). LC-MS Calculated for C 20 H 26 N 8 O is 394.22; Observed: 395.25 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.11 (bs, 2H), 7.59 (d, J=2.0 Hz, 1H), 6.67 (t, J=5.6 Hz, 1H), 6.07 (d, J=2.0 Hz, 1H), 4.62 (t, J=5.6 Hz, 1H), 4.02 (s, 2H), 3.57 (q, J=5.6 Hz, 2H), 3.17 (d, J=5.6 Hz, 2H), 2.82 (t, J=7.6 Hz, 2H), 2.56-2.43 (m, 2H), 2.25 (s, 3H), 1.12 (t, J=7.6 Hz, 3H), 0.54-0.51 (m, 2H), 0.43-0.41 (m, 2H). HPLC: Rt=11.567 min; Purity=99.39%, INT ODS 3V-C18 (4.6×250) mm, 5μ, Mobile Phase A: 0.1% Formic acid in water, Mobile Phase B: Acetonitrile.

Example 64: 7-amino-3-chloro-5-((2-(6-(1,1-difluoro-2-hydroxyethyl) pyridin-2-yl) ethyl)amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

›Abbreviations · 64 of 69

To a stirred solution of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX (0.2 g, 0.7 mmol) in 2-propanol was added 2-(6-(2-Aminoethyl)pyridin-2-yl)-2,2-difluoroethan-1-ol XXXVIII (0.3 g, 1 mmol) and triethylamine (0.8 g, 7 mmol) under N 2 and the reaction mixture was stirred at 140° C. for 16 h. The progress of reaction was monitored by TLC analysis. After completion, reaction mixture was cooled to room temperature, diluted with water (25 mL) and extracted with ethyl acetate (20 mL×3). The combined organic phase was dried over anhydrous sodium sulphate and concentrated under reduced pressure to obtain crude product. The obtained crude material was purified by reverse phase preparative HPLC (Column: Phenomenex, Kinetic C18 (250×20) mm, 5 μm. A: 10 mm Ammonium bicarbonate in water. B: Acetonitrile) to obtain the pure desired off-white solid material. Yield: 0.05 g (8.0%). LC-MS Calculated. for C 17 H 16 ClF 2 N 7 O is 407.10; Observed. 408.15 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.41 (bs, 2H), 7.89 (t, J=8.0 Hz, 1H), 7.52 (d, J=8.0 Hz, 1H), 7.45 (d, J=8.0 Hz, 1H), 7.07 (t, J=5.6 Hz, 1H), 5.49 (t, J=6.4 Hz, 1H), 4.00 (td, J=14.4, 6.8 Hz, 2H), 3.73 (q, J=6.8 Hz, 2H), 3.10 (t, J=6.8 Hz, 2H), 2.28 (s, 3H). HPLC: 97.65%,

Example 65: 7-amino-3-chloro-5-((2-(6-(1-hydroxy-2-methylpropan-2-yl)pyridin-2-yl)ethyl)amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 2-(6-(2-aminoethyl) pyridin-2-yl)-2-methylpropan-1-ol XXXII (0.4 g, 2 mmol) in 2-propanol (20 mL) were added 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX (0.3 g, 1 mmol) and triethylamine (0.4 mL, 3 mmol) under nitrogen. The resulting mixture was stirred at 120° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to room temperature and diluted with water (30 mL) then extracted with ethyl acetate (25 mL×2). The combined organic layer was washed with 25 mL of brine and passed through anhydrous sodium sulphate then concentrated under reduced pressure. The residue obtained upon removal of the solvent was subjected to silica gel (230-400 mesh) column chromatography using 0-35% ethyl acetate in n-hexane to afford the desired title compound Example 65 as off-white solid. Yield: 127 mg (30%). LC-MS: calculated. for C 19 H 22 ClN 7 O is 399.15; Observed. 400.20 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.39 (bs, 2H), 7.63 (t, J=8.0 Hz, 1H), 7.22 (d, J=8.0 Hz, 1H), 7.10 (d, J=8.0 Hz, 1H), 6.97 (t, J=5.2 Hz, 1H), 4.64 (t, J=5.2 Hz, 1H), 3.73 (q, J=6.0 Hz, 2H), 3.55 (d, J=5.6 Hz, 2H), 3.03 (t, J=6.4 Hz, 2H), 2.28 (s, 3H), 1.24 (s, 6H). HPLC Purity=97.50%,

Example 66: 7-amino-3-ethyl-5-((2-(4-(1-(hydroxymethyl) cyclopropyl)thiazol-2-yl)ethyl)amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile)

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.15 g, 0.54 mmol) in isopropanol (5 mL) were added (1-(2-(2-aminoethyl) thiazol-4-yl) cyclopropyl) methanol XXXIV (0.32 g, 1.6 mmol) and TEA (0.37 mL, 2.7 mmol) in sealed tube. The reaction mixture was stirred at 120° C. for 48 h and the progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under reduced pressure and crude was purified by flash silica gel (230-400) column chromatography using 0-35% ethyl acetate in n-hexane to afford the title compound Example 66 as a yellow sticky solid. Yield: 7.2 mg (3%). LC-MS Calculated. for C 19 H 23 N 7 OS is 397.16; Observed. 398.20 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.13 (bs, 2H), 7.15 (s, 1H), 6.79 (bs, 1H), 4.68 (bs, 1H) 3.66-3.64 (m, 4H), 3.20 (t, J=7.2 Hz, 2H), 2.45-2.43 (m, 2H), 2.25 (s, 3H), 1.12 (t, J=7.6 Hz, 4H), 0.99-0.97 (m, 3H). HPLC: 12.104 min; 98.45%, INT ODS 3V-C18 (4.6*250) mm, 5μ, Mobile Phase A: 0.1% Formic acid in water, Mobile Phase B: Acetonitrile; Flow: 1.0 mL/min.

Example 67: Synthesis of 7-amino-3-chloro-5-((2-(1-(2-(hydroxymethyl) cyclobutyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX (2.2 g, 7.7 mmol) and (2-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclobutyl) methanol XXXIX (1.5 g, 7.7 mmol) in 2-propanol (50 mL) was added triethylamine (6.4 mL, 46 mmol). The resulting mixture was heated at 120° C. for 16 h in seal tube. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and concentrated in vacuum. The residue was dissolved in water (20 mL) and extracted with ethyl acetate (50 mL×3). The combined organic layer was dried over sodium sulphate, filtered, and concentrated under reduced pressure. The crude was purified by Biotage flash silica gel (230-400) column chromatography using 0-50% ethyl acetate in n-hexane to afford the desired title compound Example 67 as an off-white solid. Yield: 2.3 g, (74%); LC_MS Calculated. for C 18 H 21 ClN 8 O is 400.15; Observed. 401.30 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.40 (bs, 2H), 7.66 (d, J=1.6 Hz, 1H), 7.00 (t, J=4.8 Hz, 1H), 6.08 (d, J=2.0 Hz, 1H), 4.60 (t, J=5.2 Hz, 1H), 4.52-4.50 (m, 1H), 3.63-3.58 (m, 2H), 3.41-3.39 (m, 2H), 2.85-2.78 (m, 3H), 2.34-2.20 (m, 5H), 1.80-1.78 (m, 1H), 1.60-1.55 (m, 1H). HPLC: 10.082 min; 99.13%, Column: INT ODS 3V-C18 (4.6*250) mm, 5μ Mobile phase A: 0.1% Formic acid in water Mobile phase B: Acetonitrile, Flow: 1.0 mL/min.

Example 68: 7-amino-5-((2-(1-(1-(amino methyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-3-chloro-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Step-1: Synthesis of (1-(3-(2-((7-amino-3-chloro-6-cyano-2-methylpyrazolo[1,5-a] pyrimidin-5-yl) amino) ethyl)-1H-pyrazol-1-yl) cyclopropyl) methyl methane sulfonate. (Example 68a)

To a solution of 7-amino-3-chloro-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile Example 37 (2.69 g, 6.95 mmol) in THF (20 mL) at 0° C. was added triethylamine (4.85 mL, 34.8 mmol) and methane sulfonyl chloride (0.6 mL, 7.65 mmol). The reaction mixture was stirred at 0° C. 3 h. The progress of the reaction was monitored by TLC analysis. After the completion, the reaction mixture was warmed to rt and diluted with NaHCO 3 solution (100 mL). The mixture was extracted with DCM (100 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound was purified by silica gel (230-400 mesh) column chromatography using ethyl acetate in hexane (0-100%) to afford the desired compound Example 68a as a light-yellow liquid. Yield: 1.8 g, (56.0%); LC_MS Calculated. for C 18 H 21 ClN 8 O 3 S is 464.11; Observed: 465.25 [M+H] + .

›Abbreviations · 65 of 69

Step-2: Synthesis of 7-amino-5-((2-(1-(1-(azidomethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-3-chloro-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (Example 68b)

To a solution of (1-(3-(2-((7-amino-3-chloro-6-cyano-2-methylpyrazolo [1,5-a] pyrimidin-5-yl) amino) ethyl)-1H-pyrazol-1-yl) cyclopropyl) methyl methane sulfonate Example 68a (1.8 g, 3.9 mmol) in N, N-dimethyl formamide (10 mL) was added sodium azide (1.0 g, 15 mmol) and heated at 80° C. for 4 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, reaction mixture was cooled to rt, diluted with water (30 mL), and extracted with ethyl acetate (50 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound was purified by silica gel (230-400 mesh) column chromatography eluted with ethyl acetate in n-hexane (0-30%) to afford the desired product Example 68b as a light-yellow solid. Yield: 1.24 g, (77.0%); LCMS Calculated. for C 17 H 18 ClN 11 is 411.14; Observed: 412.3 [M+H] + 1 HNMR (400 MHz, CDCl 3 ): δ 7.47 (d, J=2 Hz, 1H), 6.11-6.06 (m, 4H), 3.88-3.84 (m, 2H), 3.60 (s, 2H), 2.95 (t, J=6 Hz, 2H), 2.35 (s, 3H), 1.36 (t, J=6.4 Hz, 2H), 1.10 (t, J=7.2 Hz, 2H).

Step-3: 7-amino-5-((2-(1-(1-(amino methyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-3-chloro-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of 7-amino-5-((2-(1-(1-(azidomethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-3-chloro-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile Example 68b (0.3 g, 0.7 mmol) in THF (10 mL) and water (3 mL) was added triphenylphosphine (0.3 g, 1 mmol). The reaction mixture was stirred at for 10 min. This was followed by an addition of potassium hydroxide (0.04 g, 0.7 mmol) and stirred at rt for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, diluted with water (20 ml) and extracted with ethyl acetate (20 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound was purified by silica gel (60-120 mesh) column chromatography eluted with methanol in dichloromethane (0-10%) to afford the desired title product Example 68 as an off-white solid. Yield: 0.25 g, (83.0%); LCMS Calculated. for C 17 H 20 ClN 9 is 385.15; Observed: 386.3 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): δ 7.69 (d, J=2 Hz, 2H), 6.95-6.93 (m, 4H), 6.14 (d, J=2 Hz, 1H), 3.88-3.84 (m, 2H), 3.60 (s, 2H), 2.95 (t, J=6 Hz, 2H), 2.35 (s, 3H), 1.19-1.14 (m, 4H). HPLC: 7.963 min; 99.08%, INT ODS 3V-C18 (4.6*250) mm, 5μ, Mobile Phase A: 0.1% Formic acid in water, Mobile Phase B: Acetonitrile

Example 69: 7-amino-3-bromo-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of 7-amino-3-bromo-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXXVI (0.3 g, 0.91 mmol) in isopropyl alcohol (15 mL) were added (1-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclopropyl) methanol (0.33 g, 1.82 mmol) and triethylamine (0.76 mL, 5.45 mmol) and stirred at 150° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, added water (50 mL) and extracted with ethyl acetate (2×25 mL). The combined organic layer was dried over anhydrous sodium sulphate, filtered, and concentrated under reduced pressure to afford the crude compound as a brown semi-solid. The crude compound was purified by silica gel (230-400 mesh) column chromatography with methanol in dichloromethane (0-5%) to afford the desired compound Example 69 as a brown solid. Yield: 0.1 g, (25.5%); LC_MS Calculated. for C 17 H 19 BrN 8 O is 430.08; Observed: 431.25 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.39 (bs, 2H), 7.62 (d, J=2.4 Hz, 1H), 6.95 (t, J=5.2 Hz, 1H), 6.06 (d, J=2.64 Hz, 1H), 4.88 (t, J=5.6 Hz, 1H), 3.63-3.58 (m, 4H), 2.82 (t, J=8 Hz, 2H), 2.29 (d, J=21 Hz, 3H), 1.07-0.94 (m, 4H); HPLC Purity: 10.993 min; 96.42%, INT ODS 3V-C18 (4.6*250) mm, 5μ; Mobile Phase A: 0.1% Formic acid in water, Mobile Phase B: Acetonitrile; Flow rate: 1.0 mL/min

Example 70: 7-amino-3-chloro-5-((2-(1-ethyl-2-oxo-1,2-dihydropyridin-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of 3-(2-aminoethyl)-1-ethylpyridin-2(1H)-one XLI (0.2 g, 0.7 mmol) in isopropyl alcohol (5 mL) were added 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX (0.12 g, 0.7 mmol) and triethylamine (0.5 mL, 3.50 mmol). The reaction mixture was stirred at 160° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, solids were filtered, washed with ethyl acetate (50 mL) and triturated with acetonitrile to afford the desired product as an off-white solid. Yield:0.15 g, (58.0%); LC_MS Calculated. for C 17 H 18 ClN 7 O is 371.13; Observed. 372.20 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.35 (s, 2H), 7.58-7.57 (m, 1H), 7.29 (d, J=4.4 Hz, 1H), 7.08 (t, J=5.2 Hz, 1H), 6.17 (t, J=4 Hz, 1H), 3.95-3.89 (m, 2H), 3.56-3.51 (m, 2H), 2.74 (t, J=6.4 Hz, 2H), 2.27 (s, 3H), 1.22 (t, J=8 Hz, 3H); HPLC: 95.69%.

Example 71: 7-amino-3-ethyl-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a solution of 7-amino-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a]pyrimidine-6-carbonitrile XLII (0.12 g, 0.452 mmol) in isopropyl alcohol (15 mL) were added (1-(3-(2-aminoethyl)-1H-pyrazol-1-yl)cyclopropyl)methanol XXIV (0.18 g, 0.993 mmol) and triethylamine (0.378 mL, 2.71 mmol) and the reaction mixture was stirred at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, concentrated and added water (10 mL), extracted with ethyl acetate (20 mL×3). The combined organic layer was washed with brine (20 mL), dried over anhydrous sodium sulphate, and concentrated to get the crude material. The crude compound was purified by silica gel (60-120 mesh) column chromatography with ethyl acetate in n-hexane (0-60%) to afford the desired compound Example 71 as an off-white solid. Yield: 48.3 mg, (29.2%); LC_MS Calculated. for C 18 H 22 N 8 O is 366.19; Observed. 367.25 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.23 (s, 2H), 7.82 (s, 1H), 7.62 (s, 1H), 6.66 (s, 1H), 6.04 (s, 1H), 4.87 (t, J=5.6 Hz, 1H), 3.59 (t, J=5.6 Hz, 3H), 2.82 (t, J=7.2 Hz, 2H), 1.20-1.17 (m, 2H), 1.08 (d, J=6.4 Hz, 2H), 0.97-0.94 (m, 2H). HPLC: 98.98%.

›Abbreviations · 66 of 69

Example 72: 7-amino-3-bromo-5-((2-(6-(hydroxymethyl) pyridine-2-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of 7-amino-3-bromo-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XXXVI (0.3 g, 0.9 mmol) and (6-(2-aminoethyl) pyridin-2-yl) methanol XLIII (0.2 g, 1 mmol) in isopropyl alcohol (10 mL) was added triethylamine (0.6 mL, 5 mmol) and the reaction mixture was stirred at 130° C. for 36 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was cooled to rt, diluted with water (20 mL) and extracted with ethyl acetate (20 mL×3). The combined organic layer was washed with brine (10 mL), dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound purified by silica gel (230-400 mesh) column chromatography using ethyl acetate in n-hexane (0-50%) to afford the desired compound Example 72 as an off-white solid. Yield: 21.6 mg, (5.0%); LCMS Calculated. for C 6 H 16 BrN 7 O is 401.06; Observed. 402.1 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.39 (s, 2H), 7.73 (t, J=8.0 Hz, 1H), 7.32 (d, J=8.0 Hz, 1H), 7.21-7.17 (m, 2H), 5.36 (t, J=4.0 Hz, 1H), 4.58 (d, J=4.0 Hz, 2H), 3.69 (d, J=4.0 Hz, 2H), 3.02 (t, J=6.4 Hz, 2H), 2.27 (s, 3H). HPLC: 95.94%.

Example 73: 7-amino-3-chloro-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-1H-pyrazol-4-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

To a solution of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX (0.2 g, 0.7 mmol) in isopropanol (5 mL) was added (1-(4-(2-aminoethyl)-1H-pyrazol-1-yl)cyclopropyl)methanol XLIV (0.4 g, 2 mmol). The mixture was purged with N 2 gas for 5 min and triethylamine (0.5 mL, 4 mmol) was added. The reaction mixture in seal tube was heated at 150° C. for 24 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to room temperature and the reaction mixture was concentrated under reduced pressure. The residue was dissolved in water (20 mL) and extracted with ethyl acetate (20 mL×2). The combined organic layer was given brine wash, dried over anhydrous sodium sulphate, and concentrated under vacuum to afford a light brown viscous liquid. The crude compound was purified by combi-flash silica gel (230-400) column chromatography using 10-60% ethyl acetate in n-hexane to afford the desired compound Example 73 as an off-white solid. Yield: 33 mg (11%). LC_MS Calc. for C 17 H 19 ClN 8 O is 386.13; Obs. 387.20 [M+H] + ; 1 H NMR (400 MHz, DMSO-D 6 ): 8.38 (bs, 2H), 7.57 (s, 1H), 7.29 (s, 1H), 7.01 (bs, 1H), 4.89 (bs, 1H), 3.59 (d, J=5.6 Hz, 2H), 3.51-3.45 (m, 2H), 2.69 (bs, 2H), 2.28 (s, 3H), 1.04 (bs, 2H), 0.97 (bs, 2H). HPLC: 99.30%, 8.430 min;)(Bridge C18 (4.6*150) mm, 5μ, Mobile Phase A: 10 mM ammonium bicarbonate in water, Mobile Phase B: Acetonitrile.

Example 74: 7-amino-3-(cyclopropyl methyl)-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a solution of 7-amino-3-(cyclopropyl methyl)-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile V (0.2 g, 0.7 mmol) and (1-(3-(2-aminoethyl)-1H-pyrazol-1-yl)cyclopropyl)methanol XXIV (0.2 g, 1 mmol) in isopropyl alcohol (10 mL) was added triethylamine (0.5 mL, 4 mmol) and the reaction mixture was stirred at 140° C. for 16 h. The progress of the reaction was monitored by TLC analysis. After the completion of the reaction, the reaction mixture was concentrated, added water (20 mL) and extracted with ethyl acetate (25 mL×3). The combined organic layer was washed with brine (25 mL), dried over anhydrous sodium sulphate, and concentrated under reduced pressure to get the crude material. The crude compound was purified by reverse phase preparative HPLC method by using Column: Inertsil (20×250 mm), 5mic. Channel A: 0.1% Formic acid in H 2 O. Channel B: Acetonitrile: Methanol: 1:1 to afford the desired compound Example 74 as an off-white solid. Yield:0.063 g, (20.0%); LC_MS Calculated. for C 21 H 26 N 8 O is 406.22; Observed: 407.25 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.11 (s, 2H), 7.62 (s, 1H), 6.63 (bs, 1H), 6.03 (s, 1H), 4.88 (t, J=5.6 Hz, 1H), 3.6-3.53 (m, 4H), 2.81 (t, J=7.2 Hz, 2H), 2.39 (d, J=6.4 Hz, 2H), 2.27 (s, 3H), 1.08 (s, 2H), 0.96 (s, 3H), 0.35 (d, J=7.6 Hz, 2H), 0.17 (d, J=4.4 Hz, 2H). HPLC: 98.50%, 11.831 min; Column: INT ODS 3V-C18 (4.6*250) mm, 5μ; Mobile phase A: 0.1% Formic acid in water; Mobile phase B: Acetonitrile; Flow: 1.0 mL/min

Example 75: 7-amino-3-chloro-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-5-methyl-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

A seal tube was charged with 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX (160 mg, 0.560 mmol), (1-(3-(2-aminoethyl)-5-methyl-1H-pyrazol-1-yl) cyclopropyl) methanol XLV (186 mg, 0.952 mmol) and 2-propanol (25 mL). The resulting mixture was purged with N 2 gas for 5 min and triethyl amine (0.47 mL, 3.36 mmol) was added. The reaction mixture was stirred at 140° C. for 16 h. The progress of reaction was monitored by TLC analysis. After completion, water (20 mL) was added, and the resulting mixture was extracted with ethyl acetate (20 mL×3). The combined organic layer was given brine wash, dried over anhydrous sodium sulphate, and concentrated to get crude material. The crude was purified by Combi-flash silica gel (230-400 mesh) column chromatography using 10-60% ethyl acetate in n-hexane to afford the desired compound Example 75 as an off-white solid. Yield: (30 mg, 13.39%); LC-MS Calculated. for C 18 H 21 ClN 8 O is 400.15; Observed: 401.20 [M+H] + . 1 H NMR (400 MHz, DMSO-d 6 ): δ 8.39 (s, 2H), 6.91 (bs, 1H), 5.84 (s, 1H), 4.90 (bs, 1H), 3.58-3.53 (m, 4H), 2.74 (t, J=7.2 Hz, 2H), 2.30-2.26 (m, 6H), 1.03 (d, J=9.2 Hz, 4H); HPLC: 10.892 min; 97.34%, INT ODS 3V-C18 (4.6*250) mm, 5μ, Mobile Phase A: 0.1% Formic acid in water, Mobile Phase B: Acetonitrile: Flow: 1.0 mL/min

›Abbreviations · 67 of 69

Example 76: 7-amino-3-ethyl-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-5-methyl-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

A seal tube was charged with 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (170 mg, 0.609 mmol), (1-(3-(2-aminoethyl)-5-methyl-1H-pyrazol-1-yl) cyclopropyl) methanol XLV (185 mg, 0.947 mmol) and 2-propanol (20 mL). The resulting mixture was purged with N 2 gas with vigorous stirring and triethyl amine (0.51 mL, 3.65 mmol) was added. The reaction mixture was stirred at 140° C. for 3 days and progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to rt, and water (20 mL) was added. The mixture was extracted with ethyl acetate (15 mL×3). The combined organic layer was given brine wash and dried over anhydrous sodium sulphate. The crude material obtained upon removal of the solvent under vacuo was subjected to flash chromatography using Biotage system and 0-4% methanol in DCM as elluent to afford the desired compound Example 76 as an off-white solid. Yield: (70 mg, 29%); LC-MS Calculated for C 20 H 26 N 8 O is 394.22; Observed: 395.25 [M + +1]. 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.09 (bs, 2H), 6.56 (bs, 1H), 5.82 (s, 1H), 4.89 (t, J=5.6 Hz, 1H), 3.54-3.51 (m, 4H), 2.73 (d, J=6.4, 2H), 2.33-2.25 (m, 6H), 1.13-1.02 (m, 7H). HPLC purity: 11.193 min; 98.87%, INT ODS 3V-C18 (4.6*250) mm, 5μ, Mobile Phase A: 0.1% Formic acid in water, Mobile Phase B: Acetonitrile.

Example 77: Racemic (+−)-7-amino-3-ethyl-5-((2-(1-(2-(hydroxymethyl) cyclobutyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Example 78. (+)-7-amino-3-ethyl-5-((2-(1-(2-(hydroxymethyl)cyclobutyl)-1H-pyrazol-3-yl)ethyl)amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Example 79: (−)-7-amino-3-ethyl-5-((2-(1-(2-(hydroxymethyl)cyclobutyl)-1H-pyrazol-3-yl)ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

A stirred solution of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (20.0 g, 71.6 mmol) and (2-(3-(2-aminoethyl)-1H-pyrazol-1-yl) cyclobutyl) methanol XXXIX (28.0 g, 143 mmol) in 2-propanol (50 mL), was added triethylamine (60.0 mL, 430 mmol). The resulting reaction mixture was heated in seal tube at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and diluted with water (200 mL) and extracted with ethyl acetate (200 mL×3). The combined organic layer was washed with saturated brine solution (200 mL), dried over anhydrous Na 2 SO 4 , filtered and evaporated under reduced pressure. The crude compound was purified by combi-flash column chromatography (silica 230-400) by eluting with 0-50% ethyl acetate in n-hexane to afford the desired compound Example 77 as off-white solid. Yield: 13.9 g, (49%). LC-MS Calculated. for C 20 H 26 N 8 O is 394.22; Observed. 395.35 [M+H] + . 1 HNMR (400 MHz, DMSO-D 6 ): δ 8.11 (bs, 2H), 7.65 (m, 1H), 6.66 (bs, 1H), 6.07 (m, 1H), 4.60 (t, J=5.2 Hz, 1H), 4.54-4.47 (m, 1H), 3.58-3.57 (m, 2H), 3.42-3.39 (m, 2H), 2.84-2.81 (m, 3H), 2.47-2.41 (m, 2H), 2.34-2.18 (m, 5H), 1.83-1.76 (m, 1), 1.61-1.56 (m, 1H), 1.12 (t, J=7.6 Hz, 3H). HPLC: 11.728 min; 96.66%, Column: Column: INT ODS 3V-C18 (4.6*250) mm, 5μ Mobile phase A: 0.1% Formic acid in water Mobile phase B: Acetonitrile Flow: 1.0 mL/min.

The above racemic compound was subjected to chiral separation by SFC {The sample was dissolved in ˜165 mL of Tetrahydrofuran: Methanol (1:1)}, Column Chiral Pak IG (250*21) mm, 5.0 μm; Mobile phase 75:25 (A:B). A=Liquid CO 2 , B=0.1% Isopropyl amine in Isopropyl alcohol: Acetonitrile (1:1), Flow rate: 25 mL/min; Wavelength 254 nm to yield 6.2 g of peak 1, Example 78, [α] D =+43.15°, c=0.103, MeOH) and 3.9 g of peak 2, Example 79, [α] D =−33.63°, c=0.101, MeOH) as off-white solids, respectively.

Peak-1; Example 78: (+)-7-amino-3-ethyl-5-((2-(1-(2-(hydroxymethyl) cyclobutyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile, LCMS: Calculated. for C 20 H 26 N 8 O is 394.22; Observed. 395.35 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.10 (bs, 2H), 7.65-7.64 (m, 1H), 6.66-6.63 (m, 1H), 6.06 (m, 1H), 4.59 (m, 1H), 4.53-4.47 (m, 1H), 3.60-3.55 (m, 2H), 3.40 (m, 2H), 2.84-2.81 (m, 3H), 2.46-2.43 (m, 2H), 2.34-2.18 (m, 5H), 1.82-1.75 (m, 1H), 1.63-1.56 (m, 1H), 1.12 (t, J=7.6 Hz, 3H). HPLC: 11.711 min; 99.76%, Column: INT ODS 3V-C18 (4.6*250) mm, 5μ; Mobile phase A: 0.1% Formic acid in water Mobile phase B: Acetonitrile; Flow: 1.0 mL/min. Chiral HPLC; Column Name: Chiral Pak IG (4.6*250 mm), 5 μm; Co-Solvent Name: IPA:MeCN (1:1)+0.1% TEA; Total flow rate: 3 g/mL; % of Co-Solvent: 30; Temperature: 25° C., Back Pressure: 1500 psi.

Peak-2; Example 79; (−)-7-amino-3-ethyl-5-((2-(1-(2-(hydroxymethyl) cyclobutyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile, LC-MS Calculated: for C 20 H 26 N 8 O is 394.22; Observed: 395.35 [M+1] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.10 (bs, 2H), 7.64 (m, 1H), 6.65-6.64 (bs, 1H), 6.06 (m, 1H), 4.60-4.58 (m, 1H), 4.53-4.47 (m, 1H), 3.60-3.55 (m, 2H), 3.41-3.37 (m, 2H), 2.84-2.79 (m, 3H), 2.47-2.43 (m, 2H), 2.34-2.18 (m, 5H), 1.80-1.76 (m, 1), 1.60-1.56 (m, 1H), 1.12 (t, J=7.6 Hz, 3H). HPLC: 11.721 min; 98.87%, Column: Column: INT ODS 3V-C18 (4.6*250) mm, 5μ Mobile phase A: 0.1% Formic acid in water Mobile phase B: Acetonitrile Flow: 1.0 mL/min; Chiral HPLC; Method information: Column Name: chiral Pak IG (4.6*250 mm), 5 μm; Co-Solvent Name: IPA:MeCN (1:1)+0.1% TEA; Total flow rate: 3 g/mL % of Co-Solvent: 30% Temperature: 25° C., ABPR Pressure: 1500 psi.

Example 80: 7-amino-3-ethyl-5-((2-(1-(1-(hydroxymethyl) cyclopropyl)-5-methyl-1H-pyrazol-3-yl) ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XLII (150 mg, 0.565 mmol) and (1-(3-(2-aminoethyl)-5-methyl-1H-pyrazol-1-yl) cyclopropyl) methanol XLV (221 mg, 1.13 mmol) in 2-propanol (5 mL) was added triethylamine (0.474 mL, 3.39 mmol). The resulting mixture was purged with N 2 gas, and heated at 140° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was diluted with water (100 mL) and extracted with ethyl acetate (100 mL×3). The organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude was purified by combi flash silica gel (230-400 mesh) column chromatography using 0-3% MeOH in DCM to afford the desired compound Example 80 as off-white solid. Yield: (38 mg, 17.67%); LCMS Calculated. for C 19 H 24 N 8 O is 380.21; Observed: 381.35 [M + +1]. 1 HNMR (400 MHz, DMSO-d 6 ): δ 8.23 (bs, 2H), 7.82 (s, 1H), 6.64 (t, J=5.2 Hz, 1H), 5.82 (s, 1H), 4.90 (t, J=6.0 Hz, 1H), 3.58-3.51 (m, 4H), 2.74 (t, J=7.2 Hz, 2H), 2.30 (s, 3H), 1.19 (t, J=7.6 Hz, 3H), 1.03 (d, J=8.8 Hz, 4H). HPLC: 8.84 min; 98.79%, INT ODS 3V-C18 (4.6*250) mm, 5μ, Mobile Phase A: 0.1% Formic acid in water, Mobile Phase B: Acetonitrile

›Abbreviations · 68 of 69

Example 81: 7-amino-3-ethyl-5-((2-(1-(1-hydroxypropan-2-yl)-1H-pyrazol-3-yl)ethyl)amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

In a seal tube 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (120 mg, 430 μmol) and 2-(3-(2-aminoethyl)-1H-pyrazol-1-yl) propan-1-ol XLVII (109 mg, 644 μmol) were dissolved in isopropanol (15 mL) under inert atmosphere. To the reaction was added TEA (0.36 mL, 2.58 mmol) was added and the reaction mixture was stirred at 150° C. for 72 h. The progress of reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and concentrated under reduced pressure. The crude material subjected to silica gel (230-400) flash column chromatography (Biotage) using 0-3% methanol in DCM to afford the desired compound Example 81 as off-white solid. Yield: 30 mg (18.9%). LC-MS Calculated. for C 18 H 24 N 8 O is 368.20; Observed.: 369.40 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.10 (bs, 2H), 7.60 (bs, 1H), 6.65 (bs, 1H), 6.05 (d, J=1.6 Hz, 1H), 4.84 (t, J=5.2 Hz, 1H), 4.26 (q, J=6.0 Hz, 1H), 3.67-3.55 (m, 4H), 2.82 (t, J=7.2 Hz, 2H), 2.47-2.45 (m, 2H), 2.25 (s, 3H), 1.36 (d, J=7.2 Hz, 3H) 1.12 (t, J=7.6 Hz, 3H). HPLC: 11.091 min; 97.12%, INT ODS 3V-C18 (4.6*250) mm, 5μ; Mobile Phase A: 0.1% Formic acid in water, Mobile Phase B: Acetonitrile; Racemic compound: Chiral HPLC: Peak-1: 18.077 min; 50.27%, Peak-2: 20.226 min; 49.73%; Column: Chiral Pak Iowa (250*4.6) mm, 5μ; Mobile phase: 0.1% TFA in Hex, B: IPA (90:10) Flow: 1.0 mL/min.

Example 82: (+−)-7-amino-3-chloro-5-((2-(1-((3-hydroxycyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Example 83: (+)-7-amino-3-chloro-5-((2-(1-((3-hydroxycyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Example 84: (−)-7-amino-3-chloro-5-((2-(1-((3-hydroxycyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

Step-1: 7-amino-3-chloro-2-methyl-5-((2-(1-((3-oxocyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (Example 82a)

To a stirred solution of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX (0.5 g, 2 mmol) and 3-((3-(2-aminoethyl)-1H-pyrazol-1-yl) methyl) cyclobutan-1-one XLVI (0.8 g, 4 mmol) in IPA (10 mL), in sealed tube, was added TEA (1.4 mL, 10 mmol). The reaction was stirred at 130° C. for 16 h and progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and diluted with water (20 mL). The resulting mixture was extracted with ethyl acetate (20 mL×3). The combined organic layer was washed with brine, dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude compound was subjected to silica gel (230-400) combi-flash column chromatography using 0-3% methanol in DCM to afford the desired compound Example 82a as a pale-yellow solid. Yield: 0.3 g crude (43%).

Step-2: 7-amino-3-chloro-5-((2-(1-((3-hydroxycyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile (82)

To a stirred solution of 7-amino-3-chloro-2-methyl-5-((2-(1-((3-oxocyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile Example 82a (0.3 g, 0.8 mmol) in methanol (5 mL) was added sodium borohydride (0.01 g, 0.4 mmol) in portions at 0° C. The resulting reaction mixture was stirred at rt for 1 h. The reaction was monitored by TLC analysis. After completion, the reaction mixture concentrated under vacuo and the residue was dissolved in water (15 mL). The mixture was extracted with DCM (10 mL×3). Combined organic layer was dried over anhydrous Na 2 SO 4 , filtered and concentrated under reduced pressure. The crude compound was purified by combi-flash column chromatography (230-400) using 0-10% methanol in DCM to afford the racemic compound Example 82 as off-white solid. Yield: 21 mg, (29%).

The above racemic compound was subjected to chiral separation by chiral HPLC separation (Chiral Pak IC (250*21) mm, 5μ Mobile phase A: 0.1% DEA In Hex, B: EtOH (90:10) Flow: 1.0 mL/min) to yield 6.2 g of peak 1 [α] D =+43.15°, c=0.103, MeOH) and 3.9 g of peak 2 [α] D =−33.63°, c=0.101, MeOH) as off-white solids, respectively.

Example 83a: Peak-1 Yield: 51 mg, (40%); LCMS Calculated. for C 18 H 21 ClN 8 O is 400.87; Observed. 401.30 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.39 (bs, 2H), 7.56 (d, J=2.0 Hz, 1H), 7.01-6.99 (bs, 1H), 6.06 (d, J=2.0 Hz, 1H), 4.98 (d, J=6.4 Hz, 1H), 4.02 (d, J=6.8 Hz, 2H), 3.93-3.88 (m, 1H), 3.62-3.57 (m, 2H), 2.80 (t, J=7.2 Hz, 2H), 2.28 (s, 3H), 2.20-2.10 (m, 3H), 1.58-1.54 (m, 2H). HPLC: 10.906 min; 99.15%, Column: Column: INT ODS 3V-C18 (4.6*250) mm, 5μ Mobile phase A: 0.1% Formic acid in water Mobile phase B: Acetonitrile Flow: 1.0 ml/min. Chiral HPLC; 19.597 min; 100%, Column: Chiral Pak IC (250*4.6) mm, 5μ, Mobile phase: A: 0.1% DEA in Hex, B: EtOH (90:10) Flow: 1.0 mL/min.

Example 84: Peak-2; Yield: 16 mg, (20%); LC_MS Calculated. for C 18 H 21 ClN 8 O is 400.87; Observed. 401.35 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.39 (bs, 2H), 7.62 (d, J=2.0 Hz, 1H), 7.01 (t, J=5.2 Hz, 1H), 6.07 (d, J=1.6 Hz, 1H), 4.97 (d, J=6.0 Hz, 1H), 4.21-4.16 (m, 1H), 4.06 (d, J=7.6 Hz, 2H), 3.62-3.57 (m, 2H), 2.82 (t, J=7.2 Hz, 2H), 2.28 (s, 3H), 2.01-1.99 (m, 2H), 1.91-1.84 (m, 2H). HPLC: 10.912 min; 98.65%, INT ODS 3V-C18 (4.6*250) mm, 5μ Mobile phase A: 0.1% Formic acid in water Mobile phase B: Acetonitrile; Flow: 1.0 mL/min. Chiral HPLC; 22.356 min; 95.90%; Chiral Pak IC (250*4.6) mm, 5μ; Mobile phase: A: 0.1% DEA In Hex, B: EtOH (90:10) Flow: 1.0 mL/min.

Example 85: 7-amino-3-ethyl-5-((2-(1-((3-hydroxycyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl) amino)-2-methylpyrazolo[1,5-a] pyrimidine-6-carbonitrile

Step-1: 7-amino-3-ethyl-2-methyl-5-((2-(1-((3-oxocyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl) amino) pyrazolo[1,5-a] pyrimidine-6-carbonitrile (Example 85a)

›Abbreviations · 69 of 69

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile II (0.5 g, 2 mmol) and 3-((3-(2-aminoethyl)-1H-pyrazol-1-yl) methyl) cyclobutan-1-one XLVI (0.8 g, 4 mmol) in IPA (10 mL), in sealed tube, was added TEA (1.4 mL, 10 mmol). The reaction mixture was stirred at 130° C. for 24 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was cooled to rt and diluted with water (20 mL). The mixture was extracted with ethyl acetate (20 mL×3). The combined organic layer was washed with brine, dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude compound was purified by combi-flash column chromatography (230-400) using 0-10% methanol in DCM to afford the desired compound Example 85a as an off-white solid. Yield: 273 mg, (39%); LC_MS Calculated. for C 20 H 24 N 8 O is 392.20; Obs. 393.35 [M+H] + .

Step-2: 7-amino-3-ethyl-5-((2-(1-((3-hydroxycyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl)amino)-2-methylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-ethyl-2-methyl-5-((2-(1-((3-oxocyclobutyl) methyl)-1H-pyrazol-3-yl) ethyl)amino)pyrazolo[1,5-a] pyrimidine-6-carbonitrile Example 85a (0.270 g, 0.688 mmol) in methanol (5 mL) was portion wise added sodium borohydride (13.0 mg, 0.344 mmol) at 0° C. under inert atmosphere. The reaction was stirred at rt for 1 h and progress of the reaction was monitored by TLC analysis. After completion, the reaction mixture was concentrated under vacuum and the residue was diluted with water (15 mL). The mixture was extracted with DCM (10 mL×3) and the combined organic layer was dried over anhydrous Na 2 SO 4 . The solution was concentrated under reduced pressure. The crude compound was purified by combi-flash column chromatography (230-400) using 0-10% methanol in DCM to afford the desired title compound Example 85 as off-white solid. Yield: 23 mg, (19%). LC_MS Calculated. for C 20 H 26 N 8 O is 394.22; Observed. 395.40 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.10 (bs, 2H), 7.61-7.55 (m 1H), 6.66-6.62 (m, 1H), 6.05 (s, 1H), 4.98 (t, J=6.4 Hz, 1H), 4.07-4.00 (m, 2H), 3.91-3.88 (m, 1H), 3.59-3.54 (m, 2H), 2.81 (t, J=7.2 Hz, 2H), 2.46-2.43 (m, 2H), 2.24 (s, 3H), 2.20-2.10 (m, 3H), 1.92-1.88 (m, 1H), 1.58-1.54 (m, 1H), 1.12 (t, J=7.2 Hz, 3H). HPLC: 11.054 min; 96.42%; INT ODS 3V-C18 (4.6*250) mm, 5μ; Mobile phase A: 0.1% Formic acid in water Mobile phase B: Acetonitrile; Flow: 1.0 mL/min.

Example 86: 7-amino-5-((2-(1-(1-(hydroxy methyl) cyclopropyl)-5-methyl-1H-pyrazol-3-yl) ethyl)amino)-2,3-dimethylpyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-2,3-dimethyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile I (136 mg, 0.512 mmol) and (1-(3-(2-aminoethyl)-5-methyl-1H-pyrazol-1-yl) cyclopropyl) methanol XLV (200 mg, 1.02 mmol) in 2-propanol (5 mL) was added triethylamine (0.429 mL, 3.07 mmol) under inert atmosphere. The reaction mixture in seal tube was heated at 120° C. for 24 h. The progress of the reaction was monitored by TLC analysis. After completion, the reaction was cooled to room temperature and diluted with water (50 ml). The resulting mixture was extracted with ethyl acetate (50 mL×3). The combined organic layer was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure. The crude was purified by flash column chromatography using 0-3% methanol in DCM to afford the compound Example 86 as off-white solid. Yield: 25 mg, (12.8%); LCMS Calculated. for C 19 H 24 N 8 O is 380.20; Observed. 381.35 [M+H] + . 1 H NMR (400 MHz, DMSO-D 6 ): δ 8.10 (bs, 2H), 6.55 (t, J=5.2, 1H), 5.83 (s, 1H), 4.90 (t, J=6.0 Hz, 1H), 3.58-3.51 (m, 4H), 2.73 (t, J=7.2 Hz, 2H), 2.29 (s, 3H), 2.22 (s, 3H), 1.96 (s, 3H), 1.07-0.99 (m, 4H). HPLC: 10.03 min; 98.06%, Column: INT ODS 3V-C18 (4.6×250) mm, 5μ; 0.1% Formic acid in water: Mobile Phase B: Acetonitrile.

Example 87: 7-amino-3-chloro-2-methyl-5-((2-(1-methyl-2-oxo-1,2-dihydropyridin 3-yl) ethyl) amino) pyrazolo[1,5-a]pyrimidine-6-carbonitrile

To a stirred solution of 7-amino-3-chloro-2-methyl-5-(methyl sulfonyl) pyrazolo[1,5-a] pyrimidine-6-carbonitrile XX (150 mg, 0.525 mmol) in isopropanol (5 mL) were added 3-(2-aminoethyl)-1-methylpyridin-2(1H)-one XL (160 mg, 1.05 mmol) and TEA (0.366 mL, 2.63 mmol) in a sealed tube. The resulting reaction mixture was stirred at 160° C. for 48 h. The progress of the reaction was monitored by TLC analysis. After completion the reaction mixture was cooled room temperature and precipitated solid was filtered. The solid was washed with DCM (2

›Tables in the description — 5

TABLE 1 — ND—not determined.

			A 2A R IC 50	A 2B R IC 50
	A 2A R IC 50	A 2B R IC 50	(nM) (@30	(nM) (@30
	(nM) (@5	(nM) (@30	μM	μM
Example	nM NECA)	nM NECA)	Adenosine)	Adenosine)
1	10	1326	ND	ND
2	2.0	547	ND	ND
3	1014	>3000	ND	ND
4	25	1374	ND	ND
5	0.15	0.87	ND	ND
6	6.6	>3000	ND	ND
7	0.59	>3000	ND	ND
8	46	1683	ND	ND
9	ND	1131	ND	ND
10	2.3	41	ND	ND
11	6.8	43	ND	ND
12	0.9	3.7	ND	ND
13	0.25	3.4	300	2319
14	1	7	ND	ND
15	ND	102	ND	ND
16	2	53	ND	ND
17	0.15	0.87	260	2378
18	5.5	9.5	ND	ND
19	2.1	36	ND	ND
20	ND	4	ND	ND
21	3.9	13	ND	ND
22	2.5	2.2	ND	ND
23	11	130	ND	ND
24	ND	2.5	ND	ND
25	0.2	14	ND	ND
26	2.9	0.83	ND	ND
27	ND	654	ND	ND
28	31	17	ND	ND
29	1	18	ND	ND
30	ND	4.5	ND	ND
31	0.73	51	ND	ND
32	0.5	7.3	452	2126
33	0.38	0.97	206	1423
34	0.4	6	160	ND
35	0.92	16	ND	ND
36	0.8	22	250	ND
37	0.12	7	78	1767
38	0.1	4.4	178	ND
39	0.23	0.94	120	1833
40	<0.1	3.6	260	2378
41	0.88	73	ND	ND
42	0.88	76	ND	ND
43	2.3	123	ND	ND
44	1.3	2.3	ND	ND
45	0.58	2.9	935	650
46	1.9	ND	ND	ND
47	7.2	ND	ND	ND
48	2.2	51	7659	ND
49	0.3	6	635	4260
50	0.2	3.6	2161	2367
51	0.17	2.1	348	1287
52	0.54	29.3	ND	ND
53	0.3	27	ND	ND
54	0.74	5	ND	ND
55	0.74	8.6	250	3789
56	3.3	11	ND	ND
57	13	100	ND	ND
58	0.22	4.7	756	12208
59	ND	9	1083	ND
60	ND	0.94	211	ND
61	<1	24	574	ND
62	5	42	ND	ND
63	ND	7.3	810	2798
64	ND	11	284	9075
65	<1	27	227	15000
66	5	21	ND	ND
67	0.1	ND	50	1881
68	ND	ND	161	9382
69	ND	ND	68	2053
70	ND	ND	125	>15000
71	ND	ND	158	2820
72	ND	ND	362	3116
73	ND	ND	>15000	>15000
74	ND	ND	398	1076
75	ND	ND	43	7147
76	ND	ND	50	1593
77	0.1	2	81	700
78	ND	ND	200	798
79	<0.1	2	88	586
80	ND	12	83	3804
81	ND	3.6	63	1054
82	ND	7	110	2338
83	ND	ND	109	2646
84	ND	ND	102	4245
85	ND	3	211	1070
86	0.2	19	43	2554
87	ND	ND	80	1764
88	ND	ND	286	1298
89	ND	ND	66	2371
90	ND	ND	77	1830
91	ND	ND	628	1617
92	ND	ND	79	5645
93	ND	ND	50	1227
94	ND	ND	104	10000
95	1.2	53	112	1060
96	ND	ND	456	ND
97	ND	ND	451	1428
98	ND	ND	100	>15000
99	ND	ND	69	20000
100	ND	ND	139	1007
101	ND	ND	2100	>15000
102	ND	ND	804	8301
103	ND	ND	100	2592
104a	ND	ND	88	2262
104b	ND	ND	102	2607
105	ND	ND	ND	3869
106	ND	ND	75	4388
107	ND	ND	224	1548
108	ND	ND	392	1074
109	ND	ND	402	723
110	ND	ND	>15000	>15000
111	ND	ND	666	2383
112	ND	ND	84	10829
113	ND	ND	187	1051
114	ND	ND	96	4384
115	0.3	9	117	829
116	ND	ND	427	2100
117	ND	ND	66	3061
118	ND	ND	78	7849
119	ND	ND	260	3412
120	ND	ND	529	8630
121	ND	ND	245	895
122a	ND	ND	289	707
122b	ND	ND	130	521
123	ND	ND	405	1580
124	ND	ND	126	2219
125	ND	ND	767	>15000
126	ND	ND	1885	3551
127	ND	ND	161	2171
128	ND	ND	368	1110
129	ND	ND	237	5664
130	ND	ND	280	5880
131	ND	ND	1457	>15000
132	ND	ND	248	ND
133a	ND	ND	554	6685
133b	ND	ND	136	6073
134	ND	ND	475	2509
135	ND	ND	1312	2216
136	ND	ND	506	2613
137	ND	ND	820	>15000
138	ND	ND	88	2048
139	ND	ND	165	482
140	ND	ND	6019	>15000
141	ND	ND	679	4749
142	ND	ND	141	1665
143	ND	ND	667	1340
144a	ND	ND	327	821
144b	ND	ND	254	815
145	ND	ND	294	1125
146	ND	ND	255	2469
147	ND	ND	363	9076
148	ND	ND	161	2736
149	ND	ND	43	988
150	ND	ND	1153	1626
151	ND	ND	95	4614
152	ND	ND	209	1788
153	ND	ND	195	1193
154a	ND	ND	1055	1544
154b	ND	ND	1010	1411
155	ND	ND	181	429
156	ND	ND	750	2954
157	ND	ND	1669	2934
158	ND	ND	805	3347
159a	ND	ND	139	911
159b	ND	ND	457	1022
160a	ND	ND	579	1931
160b	ND	ND	341	1252
161	ND	ND	2768	3965
162a	ND	ND	1881	5793
162b	ND	ND	1233	2725
163a	ND	ND	72	11129
163b	ND	ND	82	12177
164	ND	ND	8258	>15000
165a	ND	ND	2164	>15000
165b	ND	ND	1535	9157
166	ND	ND	1424	466
167	ND	ND	100	700
168	ND	ND	90	650
ZM241385	3.5	ND	27296	16745

TABLE 5 — Metabolic stability and Intrinsic Clearance of Example 122b, Example 13 and Example 39 determined from Rat; Dog and Human in-vitro hepatocytes assay

	Rat	Dog	Human
	CLint		CLint		CLint
	(μl/min/	CLint	(μl/min/	CLint	(μl/min/	CLint
Compounds	10{circumflex over ( )}6)	T1/2	10{circumflex over ( )}6)	T1/2	10{circumflex over ( )}6)	T1/2
Example 122b	4.7	216	<4.1	>240	<4.1	>240
Example 13	34	29	9.5	107	<8.2	>120
Example 39	<8.2	>120	10.8	92	<8.2	>120

TABLE 8 — Pharmacokinetic Study design of Example 13, Example 122b and Example 39 in Mice, Rats and Dogs

	Species/Strain/	Route/	Dose	Dose volume	Formulation
Test item	Gender	condition	(mg/kg)	(mL/kg)	details
Example	Rat/SD/Male	IV bolus/Fed	3	2	5% w/v
13					ascorbic
					acid in
					water, pH
					adjusted
					to ~4.1
					using 8.4%
					NaHCO3
	Rat/SD/Male	Oral/Fasted	100	10	5% Ascorbic
					acid:10%
					TPGS:6%
					Solutol:5%
					Poloxamer:water
					q.s
	Mice/CD1/Male	Subcutaneous/Fed	5	5	5% w/v
					ascorbic
					acid in
					water + 15%
					TPGS
	Mice/CD1/Male	Oral/Fasted	30	10	10% v/v
					DMSO:5%
					v/v Ethanol:15%
					v/v TPGS in
					water:Saline
					q.s
	Dog/Beagle/Male	IV bolus/Fed	3	2	5% w/v
					L-Ascorbic
					Acid in
					water, pH
					adjusted to
					4 using
					8.4% w/v
					sodium
					bicarbonate
					(NaHCO3)
	Dog/Beagle/Male	Oral/Fasted	10	5	10% v/v
					Dimethyl
					sulfoxide
					(DMSO):5%
					v/v Ethanol:15%
					v/v TPGS in
					water:70%
					v/v sterile
					Saline.
Example	Mice/CD1/Male	IV bolus/Fed	15	5	15% v/v
39					DMSO:25%
					v/v PEG400:10%
					v/v Ethanol:50%
					v/v Normal
					saline
	Mice/CD1/Male	Oral/Fasted	30	10	10% v/v
					DMSO:30%
					v/v PEG400:10%
					v/v Ethanol:5%
					v/v tween80:45%
					v/v Saline
	Mice/CD1/Male	Oral/Fasted	30	10	10% v/v
					DMSO:5%
					v/v Ethanol:15%
					v/v TPGS in
					water:70%
					v/v normal
					saline
	Rat/SD/Male	IV bolus/Fed	15	5	15% v/v
					DMSO +
					25% v/v
					PEG400 +
					10% v/v
					Ethanol +
					50% v/v
					Normal
					saline
	Rat/SD/Male	Oral/Fasted	100	10	10% v/v
					DMSO:30%
					v/v PEG400:10%
					v/v Ethanol:5%
					v/v tween80:45%
					v/v Saline
	Dog/Beagle/Male	IV bolus/Fed	3	2	20% v/v N,
					N-Dimethyl
					acetamide
					(DMA):10%
					v/v PEG400:70%
					v/v sterile
					saline.
	Dog/B eagle/Male	Oral/Fasted	10	5	10% v/v
					DMSO:5%
					v/v Ethanol:15%
					v/v TPGS in
					water:70%
					v/v Saline.
Example	Mice/CD1/Male	Oral/Fasted	100	10	10% v/v
122b					DMSO:5%
					v/v Ethanol:15%
					v/v TPGS in
					water:Saline
					q.s
	Rat/SD/Male	IV bolus/Fed	5	2	10% v/v
					DMSO:5%
					v/v Solutol
					HS:20% v/v
					PEG400:Saline
					q.s
	Rat/SD/Male	Oral/Fasted	30	10	10% v/v
					DMSO:5%
					v/v Ethanol:15%
					v/v TPGS in
					water:Saline
					q.s

TABLE 10 — Efficacy experimental design dose

Group	Treatment	n	(mg/kg)	Route	Regimen	Duration
1	Vehicle	10	10 ml/kg	PO	BID	Day 1 to Day 21
3	PD-1	8	5	IP	Every	Day 1 to Day 21
	Antibody				3 days
4	Example 13	10	1	PO	TID	Day 1 to Day 21
5	Example 13	10	3	PO	TID	Day 1 to Day 21
6	Example 13	10	10	PO	TID	Day 1 to Day 21
7	Example 13	10	30	PO	TID	Day 1 to Day 21
TID: Dosed three times in a day at 4 h interval (ex 9.00 am, 01.00 pm and 05.00 pm)
BID:] Dosed two times in a day at ~8 h interval (ex 9.00 am and 05.00 pm)
Example 13 and vehicle were dosed by oral route every day from Day 1 to Day 21.
Anti-mouse PD-1 antibody was dosed by IP route every three days

TABLE 11 — Formulations used for the efficacy studies

Treatment	Vehicle
Example 13	30% Gelucire 44/14 + 10% Solutol HS-15 +
	30% Propylene Glycol in saline
Anti-mouse PD-1	PBS pH 7.4
Antibody (Bioxcel, USA)
Vehicle	30% Gelucire 44/14 + 10% Solutol HS-15 +
	30% Propylene Glycol in saline

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IPC · International Patent Classification

Section A — Human necessities

A61P35/00
A61K45/06

Section C — Chemistry; metallurgy

C07D487/04

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