The present invention comprises compounds of Formula I. [structure] wherein: R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , and R 9 are defined in the specification. The invention also comprises a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is rheumatoid arthritis or psoriasis. The invention also comprises a method of modulating RORγt activity in a mammal by administration of a therapeutically effective amount of at least one compound of claim 1.

Description

93 parts

›CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional of U.S. application Ser. No. 14/513,426, filed on Oct. 14, 2014, which claims priority from provisional U.S. Application No. 61/890,889, filed on Oct. 15, 2013, which are all incorporated herein by reference.

›FIELD OF THE INVENTION

The invention is directed to substituted quinoline compounds, which are modulators of the nuclear receptor RORγt, pharmaceutical compositions, and methods for use thereof. More particularly, the RORγt modulators are useful for preventing, treating or ameliorating an RORγt mediated inflammatory syndrome, disorder or disease.

›BACKGROUND OF THE INVENTION · 1 of 2

Retinoic acid-related nuclear receptor gamma t (RORγt) is a nuclear receptor, exclusively expressed in cells of the immune system, and a key transcription factor driving Th17 cell differentiation. Th17 cells are a subset of CD4 + T cells, expressing CCR6 on their surface to mediate their migration to sites of inflammation, and dependent on IL-23 stimulation, through the IL-23 receptor, for their maintenance and expansion. Th17 cells produce several proinflammatory cytokines including IL-17A, IL-17F, IL-21, and IL-22 (Korn, T., E. Bettelli, et al. (2009). “IL-17 and Th17 Cells.” Annu Rev Immunol 27: 485-517), which stimulate tissue cells to produce a panel of inflammatory chemokines, cytokines and metalloproteases, and promote recruitment of granulocytes (Kolls, J. K. and A. Linden (2004). “Interleukin-17 family members and inflammation.” Immunity 21(4): 467-76; Stamp, L. K., M. J. James, et al. (2004). “Interleukin-17: the missing link between T-cell accumulation and effector cell actions in rheumatoid arthritis” Immunol Cell Biol 82(1): 1-9). Th17 cells have been shown to be the major pathogenic population in several models of autoimmune inflammation, including collagen-induced arthritis (CIA) and experimental autoimmune encephalomyelitis (EAE) (Dong, C. (2006). “Diversification of T-helper-cell lineages: finding the family root of IL-17-producing cells.” Nat Rev Immunol 6(4): 329-33; McKenzie, B. S., R. A. Kastelein, et al. (2006). “Understanding the IL-23-IL-17 immune pathway.” Trends Immunol 27(1): 17-23.). RORγt-deficient mice are healthy and reproduce normally, but have shown impaired Th17 cell differentiation in vitro, a significantly reduced Th17 cell population in vivo, and decreased susceptibility to EAE (Ivanov, II, B. S. McKenzie, et al. (2006). “The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+T helper cells.” Cell 126(6): 1121-33.). Mice deficient for IL-23, a cytokine required for Th17 cell survival, fail to produce Th17 cells and are resistant to EAE, CIA, and inflammatory bowel disease (IBD) (Cua, D. J., J. Sherlock, et al. (2003). “Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain.” Nature 421(6924): 744-8; Langrish, C. L., Y. Chen, et al. (2005). “IL-23 drives a pathogenic T cell population that induces autoimmune inflammation.” J Exp Med 201(2): 233-40; Yen, D., J. Cheung, et al. (2006). “IL-23 is essential for T cell-mediated colitis and promotes inflammation via IL-17 and IL-6.” J Clin Invest 116(5): 1310-6.). Consistent with these findings, an anti-IL23-specific monoclonal antibody blocks development of psoriasis-like inflammation in a murine disease model (Tonel, G., C. Conrad, et al. “Cutting edge: A critical functional role for IL-23 in psoriasis.” J Immunol 185(10): 5688-91).

In humans, a number of observations support the role of the IL-23/Th17 pathway in the pathogenesis of inflammatory diseases. IL-17, the key cytokine produced by Th17 cells, is expressed at elevated levels in a variety of allergic and autoimmune diseases (Barczyk, A., W. Pierzchala, et al. (2003). “Interleukin-17 in sputum correlates with airway hyperresponsiveness to methacholine.” Respir Med 97(6): 726-33; Fujino, S., A. Andoh, et al. (2003). “Increased expression of interleukin 17 in inflammatory bowel disease.” Gut 52(1): 65-70; Lock, C., G. Hermans, et al. (2002). “Gene-microarray analysis of multiple sclerosis lesions yields new targets validated in autoimmune encephalomyelitis.” Nat Med 8(5): 500-8; Krueger, J. G., S. Fretzin, et al. “IL-17A is essential for cell activation and inflammatory gene circuits in subjects with psoriasis.” J Allergy Clin Immunol 130(1): 145-154 e9.). Furthermore, human genetic studies have shown association of polymorphisms in the genes for Th17 cell-surface receptors, IL-23R and CCR6, with susceptibility to IBD, multiple sclerosis (MS), rheumatoid arthritis (RA) and psoriasis (Gazouli, M., I. Pachoula, et al. “NOD2/CARD15, ATG16L1 and IL23R gene polymorphisms and childhood-onset of Crohn's disease.” World J Gastroenterol 16(14): 1753-8., Nunez, C., B. Dema, et al. (2008). “IL23R: a susceptibility locus for celiac disease and multiple sclerosis?” Genes Immun 9(4): 289-93; Bowes, J. and A. Barton “The genetics of psoriatic arthritis: lessons from genome-wide association studies.” Discov Med 10(52): 177-83; Kochi, Y., Y. Okada, et al. “A regulatory variant in CCR6 is associated with rheumatoid arthritis susceptibility.” Nat Genet 42(6): 515-9.).

Ustekinumab (Stelara®), an anti-p40 monoclonal antibody blocking both IL-12 and IL-23, is approved for the treatment of adult patients (18 years or older), with moderate to severe plaque psoriasis, who are candidates for phototherapy or systemic therapy. Currently, monoclonal antibodies specifically targeting only IL-23, to more selectively inhibit the Th17 subset, are also in clinical development for psoriasis (Garber K. (2011). “Psoriasis: from bed to bench and back” Nat Biotech 29, 563-566), further implicating the important role of the IL-23- and RORγt-driven Th17 pathway in this disease. Results from recent phase II clinical studies strongly support this hypothesis, as anti-IL-17 receptor and anti-IL-17 therapeutic antibodies both demonstrated high levels of efficacy in patients with chronic psoriasis (Papp, K. A., “Brodalumab, an anti-interleukin-17-receptor antibody for psoriasis.” N Engl J Med 2012 366(13): 1181-9; Leonardi, C., R. Matheson, et al. “Anti-interleukin-17 monoclonal antibody ixekizumab in chronic plaque psoriasis.” N Engl J Med 366(13): 1190-9.). Anti-IL-17 antibodies have also demonstrated clinically relevant responses in early trials in RA and uveitis (Hueber, W., Patel, D. D., Dryja, T., Wright, A. M., Koroleva, I., Bruin, G., Antoni, C., Draelos, Z., Gold, M. H., Durez, P., Tak, P. P., Gomez-Reino, J. J., Foster, C. S., Kim, R. Y., Samson, C. M., Falk, N. S., Chu, D. S., Callanan, D., Nguyen, Q. D., Rose, K., Haider, A., Di Padova, F. (2010) Effects of AIN457, a fully human antibody to interleukin-17A, on psoriasis, rheumatoid arthritis, and uveitis. Sci Transl Med 2, 5272.).

›BACKGROUND OF THE INVENTION · 2 of 2

All the above evidence supports inhibition of the Th17 pathway by modulating RORγt activity as an effective strategy for the treatment of immune-mediated inflammatory diseases.

›SUMMARY OF THE INVENTION · 1 of 2

The present invention comprises compounds of Formula I.

R 1 is azetidinyl, pyrrolyl, pyrazolyl, imidazolyl, triazolyl, thiazolyl, pyridyl, pyridyl N-oxide, pyrazinyl, pyrimidinyl, pyridazyl, piperidinyl, quinazolinyl, cinnolinyl, benzothiazolyl, indazolyl, tetrahydropyranyl, tetrahydrofuranyl, furanyl, phenyl, oxazolyl, isoxazolyl, thiophenyl, benzoxazolyl, benzimidazolyl, indolyl, thiadiazolyl, oxadiazolyl or quinolinyl; wherein said azetidinyl, pyridyl, pyridyl-N-oxide, pyrazinyl, pyrimidinyl, pyridazyl, piperidinyl, quinazolinyl, cinnolinyl, benzothiazolyl, indazolyl, imidazolyl, phenyl, thiophenyl, benzoxazolyl, benzimidazolyl, indolyl, quinolinyl, and pyrazolyl are optionally substituted with C(O)C (1-4) alkyl, C(O)NH 2 , C(O)NHC (1-2) alkyl, C(O)N(C (1-2) alkyl) 2 , NHC(O)C (1-4) alkyl, NHSO 2 C (1-4) alkyl, C (1-4) alkyl, CF 3 , CH 2 CF 3 , Cl, F, —CN, OC (1-4) alkyl, N(C (1-4) alkyl) 2 , —(CH 2 ) 3 OCH 3 , SC (1-4) alkyl, OH, CO 2 H, CO 2 C (1-4) alkyl, C(O)CF 3 , SO 2 CF 3 , OCF 3 , OCHF 2 , SO 2 CH 3 , SO 2 NH 2 , SO 2 NHC (1-2) alkyl, SO 2 N(C (1-2) alkyl) 2 , C(O)NHSO 2 CH 3 , or OCH 2 OCH 3 ; and optionally substituted with up to two additional substituents independently selected from the group consisting of Cl, C (1-2) alkyl, SCH 3 , OC (1-2) alkyl, CF 3 , —CN, and F; and wherein said triazolyl, oxazolyl, isoxazolyl, pyrrolyl, and thiazolyl are optionally substituted with up to two substituents independently selected from the group consisting of SO 2 CH 3 , SO 2 NH 2 , C(O)NH 2 , —CN, OC (1-2) alkyl, (CH 2 ) (2-3) OCH 3 , SCH 3 , CF 3 , F, Cl, and C (1-2) alkyl; and said thiadiazolyl and oxadiazolyl are optionally substituted with C (1-2) alkyl; and said pyridyl, pyridyl-N-oxide, pyrimidinyl, pyridazyl, and pyrazinyl are optionally substituted with up to three additional substituents independently selected from the group consisting of C(O)NHC (1-2) alkyl, C(O)N(C (1-2) alkyl) 2 , NHC(O)C (1-4) alkyl, NHSO 2 C (1-4) alkyl, C(O)CF 3 , SO 2 CF 3 , SO 2 NHC (1-2) alkyl, SO 2 N(C (1-2) alkyl) 2 , C(O)NHSO 2 CH 3 , SO 2 CH 3 , SO 2 NH 2 , C(O)NH 2 , —CN, OC (1-4) alkyl, (CH 2 ) (2-3) OCH 3 (including —(CH 2 ) 3 OCH 3 ), SC (1-4) alkyl, CF 3 , F, Cl, and C (1-4) alkyl;

R 2 is H, C (1-6) alkyl, —C≡CH, triazolyl, pyridyl, pyridyl-N-oxide, pyrazolyl, pyrimidinyl, oxazolyl, isoxazolyl, azetidin-3-yl, N-acetyl-azetidin-3-yl, N-methylsulfonyl-azetidin-3-yl, N-Boc-azetidin-3-yl, N-methyl-azetidin-3-yl, N-acetamidyl-azetidin-3-yl, N-acetyl piperidinyl, 1-H-piperidinyl, N-Boc-piperidinyl, N—C (1-3) alkyl-piperidinyl, N-methylsulfonyl-piperidinyl, thiazolyl, pyridazyl, pyrazinyl, 1-(3-methoxypropyl)-imidazolyl, thiadiazolyl, oxadiazolyl, or imidazolyl; wherein said imidazolyl is optionally substituted with up to three additional substituents independently selected from the group consisting of C (1-2) alkyl, SCH 3 , OC (1-2) alkyl, CF 3 , —CN, F, and Cl; and said pyridyl, pyridyl-N-oxide, pyrimidinyl, pyridazyl, and pyrazinyl, are optionally substituted with up to three additional substituents independently selected from the group consisting of SO 2 CH 3 , SO 2 NH 2 , C(O)NH 2 , —CN, OC (1-2) alkyl, (CH 2 ) (2-3) OCH 3 , SCH 3 , CF 3 , F, Cl, and C (1-2) alkyl; and said triazolyl, thiazolyl, oxazolyl and isoxazolyl are optionally substituted with up to two substituents independently selected from the group consisting of SO 2 CH 3 , SO 2 NH 2 , C(O)NH 2 , —CN, OC (1-2) alkyl, (CH 2 ) (2-3) OCH 3 , SCH 3 , CF 3 , F, Cl, and C (1-2) alkyl; and said thiadiazolyl and oxadiazolyl are optionally substituted with C (1-2) alkyl; and said pyrazolyl is optionally substituted with up to three CH 3 groups;

R 3 is H, OH, OCH 3 , or NH 2 ;

R 4 is H, or F;

R 5 is H, Cl, —CN, CF 3 , SC (1-4) alkyl, OC (1-4) alkyl, OH, C (1-4) alkyl, N(CH 3 )OCH 3 , NH(C (1-4) alkyl), N(C (1-4) alkyl) 2 , 4-hydroxy-piperidinyl, azetidin-1-yl, or fur-2-yl; provided that R 5 is not H if R 7 is OCH 3 ;

R 6 is C (1-4) alkyl-Q, OC (1-4) alkyl-Q, NA 3 A 4 , NHC (1-4) alkylQ, NHCOC (1-4) alkylQ, C(O)NA 3 A 4 , CO 2 C(CH 3 ) 3 , O-tetrahydropyranyl, O—(N-methyl)-piperidinyl, O—C (3-6) cycloalkyl, O—(N-methyl)-pyrrolidinyl, O—(N-methyl)-azetidinyl, O—(N-methyl)-aziridinyl, cyclopropyl, cyclobutyl, oxetanyl, pyrrolidinyl, cyclopentyl, tetrahydrofuranyl, cyclohexyl, 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, piperidinyl, or tetrahydropyranyl; wherein said cyclopropyl, cyclobutyl, cyclopentyl, pyrrolidinyl, piperidinyl, tetrahydropyranyl, and cyclohexyl are optionally substituted with F, C(O)C (1-3) alkyl, OC(O)C (1-4) alkyl, and C (1-4) alkyl, and up to one additional fluorine atom; provided that R 6 is not CH 2 -phenyl, CH 2 -pyridinyl, CH 2 -pyrimidinyl, CH 2 -pyrazinyl, nor CH 2 -pyridazyl;

Q is H, CF 3 , OH, SO 2 CH 3 , —CN, NA 3 A 4 , CO 2 C (1-4) alkyl, OC (1-4) alkyl, cyclopropyl, cyclobutyl, oxetanyl, cyclopentyl, tetrahydrofuranyl, pyrazolyl, isoxazolyl, imidazolyl, triazolyl, oxazolyl, thiazolyl, pyrrolidinyl, cyclohexyl, piperidinyl, tetrahydropyranyl, 1,1-dioxo-tetrahydrothiopyran-4-yl, tetrahydrothiopyran-4-yl, phenyl, pyridinyl, pyrimidinyl, pyrazinyl, or pyridazyl; wherein said cyclopropyl, cyclobutyl, cyclopentyl, pyrrolidinyl, piperidinyl, tetrahydropyranyl, and cyclohexyl are optionally substituted with F, C(O)C (1-3) alkyl, CO 2 C(CH 3 ) 3 , and C (1-4) alkyl, and up to one additional fluorine atom; and said pyrazolyl, isoxazolyl, imidazolyl, triazolyl, oxazolyl, and thiazolyl are all optionally substituted with one or two CH 3 groups; and said oxetanyl is optionally substituted with CH 3 ;

wherein

A 3 is H, or C (1-4) alkyl;

A 4 is H, C (1-4) alkyl, CH 2 -cyclopropyl, cyclopropyl, C (1-3) alkylCF 3 , CH 2 CH 2 OCH 2 CF 3 , C(O)C (1-2) alkylCF 3 ,

or C (0-1) alkyl-trifluoromethyl-cyclohexyl, or A 3 and A 4 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

wherein

q b is H, F, CF 3 , SO 2 CH 3 , OC (1-4) alkyl, pyrazol-1-yl, 3-trifluoromethyl-pyrazol-1-yl, imidazol-1-yl, or triazolyl; q c is H, F, CF 3 , OC (1-4) alkyl, or OH; q d is H, CH 2 CF 3 , C (1-4) alkyl, C(O)C (1-4) alkyl, phenyl, CO 2 C(CH 3 ) 3 , SO 2 C (1-4) alkyl, CH 2 CH 2 CF 3 , CH 2 -cyclopropyl, CH 2 -phenyl, or C (3-6) cycloalkyl;

›SUMMARY OF THE INVENTION · 2 of 2

provided that if R 6 is OCH 2 -Q, then Q may not be OH, nor NA 3 A 4 ;

R 7 is H, Cl, —CN, C (1-4) alkyl, cyclopropyl, cyclobutyl, OC (1-4) alkylCF 3 , OCF 3 , OCHF 2 , OCH 2 CH 2 OC (1-4) alkyl, CF 3 , SCH 3 , C (1-4) alkylNA 1 A 2 (including CH 2 NA 1 A 2 ), CH 2 OC (2-3) alkylNA 1 A 2 , NA 1 A 2 , C(O)NA 1 A 2 , CH 2 NHC (2-3) alkylNA 1 A 2 , CH 2 N(CH 3 )C (2-3) alkylNA 1 A 2 , NHC (2-3) alkylNA 1 A 2 , N(CH 3 )C (2-4) alkylNA 1 A 2 , OC (2-4) alkylNA 1 A 2 , OC (1-4) alkyl, OCH 2 -(1-methyl)-imidazol-2-yl, thiophenyl, furyl, pyrazolyl, imidazolyl, pyridyl, pyridazyl, pyrazinyl, pyrimidinyl, indazolyl, phenyl, or

wherein said phenyl, thiophenyl, furyl, pyrazolyl, imidazolyl, pyridyl, pyridazyl, pyrazinyl, pyrimidinyl, and indazolyl are optionally substituted with up to three substituents independently selected from the group consisting of F, Cl, CH 3 , CF 3 , and OCH 3 ;

A 1 is H, or C (1-4) alkyl; A 2 is H, C (1-4) alkyl, C (1-4) alkylOC (1-4) alkyl, C (1-4) alkylOH, C(O)C (1-4) alkyl, or OC (1-4) alkyl; or A 1 and A 2 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

R a is H, OC (1-4) alkyl, CH 2 OH, NH(CH 3 ), N(CH 3 ) 2 , NH 2 , CH 3 , F, CF 3 , SO 2 CH 3 , or OH;

R b is H, CO 2 C(CH 3 ) 3 , C (1-4) alkyl, C(O)C (1-4) alkyl, SO 2 C (1-4) alkyl, CH 2 CH 2 CF 3 , CH 2 CF 3 , CH 2 -cyclopropyl, phenyl, CH 2 -phenyl, or C (3-6) cycloalkyl;

R 8 is H, C (1-3) alkyl (including CH 3 ), OC (1-3) alkyl (including OCH 3 ) CF 3 , NH 2 , NHCH 3 , —CN, or F;

R 9 is H, or F;

and pharmaceutically acceptable salts thereof.

›DETAILED DESCRIPTION OF THE INVENTION · 1 of 6

The present invention comprises compounds of Formula I.

R 3 is H, OH, OCH 3 , or NH 2 ;

R 4 is H, or F;

R 6 is C (1-4) alkyl-Q, OC (1-4) alkyl-Q, NA 3 A 4 , NHC (1-4) alkylQ, NHCOC (1-4) alkylQ, C(O)NA 3 A 4 , C(O)OC (1-4) alkyl, O-tetrahydropyranyl, O—(N-methyl)-piperidinyl, O—C (3-6) cycloalkyl, O—(N-methyl)-pyrrolidinyl, O—(N-methyl)-azetidinyl, O—(N-methyl)-aziridinyl, cyclopropyl, cyclobutyl, oxetanyl, pyrrolidinyl, cyclopentyl, tetrahydrofuranyl, cyclohexyl, 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, piperidinyl, or tetrahydropyranyl; wherein said cyclopropyl, cyclobutyl, cyclopentyl, pyrrolidinyl, piperidinyl, tetrahydropyranyl, and cyclohexyl are optionally substituted with F, C(O)C (1-3) alkyl, CO 2 C(CH 3 ) 3 , and C (1-4) alkyl, and up to one additional fluorine atom; provided that R 6 is not CH 2 -phenyl, CH 2 -pyridinyl, CH 2 -pyrimidinyl, CH 2 -pyrazinyl, nor CH 2 -pyridazyl;

wherein

A 3 is H, or C (1-4) alkyl;

A 4 is H, C (1-4) alkyl, CH 2 -cyclopropyl, cyclopropyl, C (1-3) alkylCF 3 , CH 2 CH 2 OCH 2 CF 3 , C(O)C (1-2) alkyCF 3 ,

or C (0-1) alkyl-trifluoromethyl-cyclohexyl, or A 3 and A 4 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

wherein

q b is H, F, CF 3 , SO 2 CH 3 , OC (1-4) alkyl, pyrazol-1-yl, or 3-trifluoromethyl-pyrazol-1-yl, imidazol-1-yl, or triazolyl; q c is H, F, CF 3 , OC (1-4) alkyl, or OH; q d is H, CH 2 CF 3 , C (1-4) alkyl, C(O)C (1-4) alkyl, phenyl, CO 2 C(CH 3 ) 3 , SO 2 C (1-4) alkyl, CH 2 CH 2 CF 3 , CH 2 -cyclopropyl, CH 2 -phenyl, or C (3-6) cycloalkyl; provided that if R 6 is OCH 2 -Q, then Q may not be OH, nor NA 3 A 4 ; R 7 is H, Cl, —CN, C (1-4) alkyl, cyclopropyl, cyclobutyl, OC (1-4) alkylCF 3 , OCF 3 , OCHF 2 , OCH 2 CH 2 OC (1-4) alkyl, CF 3 , SCH 3 , C (1-4) alkylNA 1 A 2 (including CH 2 NA 1 A 2 ), CH 2 OC (2-3) alkylNA 1 A 2 , NA 1 A 2 , C(O)NA 1 A 2 , CH 2 NHC (2-3) alkylNA 1 A 2 , CH 2 N(CH 3 )C (2-3) alkylNA 1 A 2 , NHC (2-3) alkylNA 1 A 2 , N(CH 3 )C (2-4) alkylNA 1 A 2 , OC (2-4) alkylNA 1 A 2 , OC (1-4) alkyl, OCH 2 -(1-methyl)-imidazol-2-yl, thiophenyl, furyl, pyrazolyl, imidazolyl, pyridyl, pyridazyl, pyrazinyl, pyrimidinyl, indazolyl, phenyl, or

›DETAILED DESCRIPTION OF THE INVENTION · 2 of 6

R a is H, OC (1-4) alkyl, CH 2 OH, NH(CH 3 ), N(CH 3 ) 2 , NH 2 , CH 3 , F, CF 3 , SO 2 CH 3 , or OH;

R b is H, CO 2 C(CH 3 ) 3 , C (1-4) alkyl, C(O)C (1-4) alkyl, SO 2 C (1-4) alkyl, CH 2 CH 2 CF 3 , CH 2 CF 3 , CH 2 -cyclopropyl, phenyl, CH 2 -phenyl, or C (3-6) cycloalkyl;

R 8 is H, C (1-3) alkyl (including CH 3 ), OC (1-3) alkyl (including OCH 3 ) CF 3 , NH 2 , NHCH 3 , —CN, or F;

R 9 is H, or F;

and pharmaceutically acceptable salts thereof;

In another embodiment of the invention:

R 1 is pyrrolyl, pyrazolyl, imidazolyl, triazolyl, thiazolyl, pyridyl, pyridyl N-oxide, pyrazinyl, pyrimidinyl, pyridazyl, piperidinyl, tetrahydropyranyl, phenyl, oxazolyl, isoxazolyl, thiophenyl, benzoxazolyl, or quinolinyl; wherein said pyridyl, pyridyl N-oxide, piperidinyl, imidazolyl, phenyl, thiophenyl, benzoxazolyl, pyrazolyl, pyrazinyl, pyrimidinyl, pyridazyl, or quinolinyl are optionally substituted with C(O)C (1-4) alkyl, C(O)NH 2 , C (1-4) alkyl, CF 3 , CH 2 CF 3 , Cl, F, —CN, OC (1-4) alkyl, N(C (1-4) alkyl) 2 , —(CH 2 ) 3 OCH 3 , SC (1-4) alkyl, OH, CO 2 H, CO 2 C (1-4) alkyl, OCF 3 , OCHF 2 , SO 2 CH 3 , SO 2 NH 2 , or OCH 2 OCH 3 ; and optionally substituted with up to two additional substituents independently selected from the group consisting of Cl, C (1-2) alkyl (including CH 3 ), SCH 3 , OC (1-2) alkyl (including OCH 3 ), CF 3 , —CN, and F; and wherein said triazolyl, oxazolyl, isoxazolyl, pyrrolyl, and thiazolyl are optionally substituted with up to two substituents independently selected from the group consisting of SO 2 CH 3 , SO 2 NH 2 , C(O)NH 2 , —CN, OC (1-2) alkyl, (CH 2 ) (2-3) OCH 3 , SCH 3 , CF 3 , F, Cl, and C (1-2) alkyl (including CH 3 ); and said pyridyl, and pyridyl-N-oxide are optionally substituted with up to three additional substituents independently selected from the group consisting of SO 2 CH 3 , SO 2 NH 2 , C(O)NH 2 , —CN, OC (1-4) alkyl, (CH 2 ) (2-3) OCH 3 (including —(CH 2 ) 3 OCH 3 ), SC (1-4) alkyl, CF 3 , F, Cl, and C (1-4) alkyl; R 2 is H, C (1-6) alkyl (including C (1-4) alkyl), —C≡CH, 1-methyl triazolyl, pyridyl, pyridyl-N-oxide, 1-methyl pyrazolyl, pyrimidinyl, oxazolyl, isoxazolyl, azetidin-3-yl, N-acetyl-azetidin-3-yl, N-methylsulfonyl-azetidin-3-yl, N-Boc-azetidin-3-yl, N-acetyl piperidinyl, 1-H-piperidinyl, N-Boc-piperidinyl, N—C (1-3) alkyl-piperidinyl (including N—C (1-2) alkyl-piperidinyl), N-methyl sulfonyl-piperidinyl, thiazolyl, pyridazyl, pyrazinyl, 1-(3-methoxypropyl)-imidazolyl, or 1-C (1-2) alkyl imidazolyl; wherein said 1-C (1-2) alkyl imidazolyl is optionally substituted with up to two additional substituents independently selected from the group consisting of C (1-2) alkyl (including CH 3 ), SCH 3 , OC (1-2) alkyl, CF 3 , —CN, F, and Cl; and said pyridyl, and pyridyl-N-oxide are optionally substituted with up to three additional substituents independently selected from the group consisting of SO 2 CH 3 , SO 2 NH 2 , C(O)NH 2 , —CN, OC (1-2) alkyl (including OCH 3 ), (CH 2 ) (2-3) OCH 3 , SCH 3 , CF 3 , F, Cl, and C (1-2) alkyl (including CH 3 ); and said thiazolyl, oxazolyl and isoxazolyl are optionally substituted with up to two substituents independently selected from the group consisting of SO 2 CH 3 , SO 2 NH 2 , C(O)NH 2 , —CN, OC (1-2) alkyl, (CH 2 ) (2-3) OCH 3 , SCH 3 , CF 3 , F, Cl, and C (1-2) alkyl (including CH 3 ); and said 1-methyl pyrazolyl is optionally substituted with up to two additional CH 3 groups; R 3 is H, OH, OCH 3 , or NH 2 ; R 4 is H, or F; R 5 is H, Cl, —CN, CF 3 , SC (1-4) alkyl, OC (1-4) alkyl, OH, C (1-4) alkyl, N(CH 3 )OCH 3 , NH(C (1-4) alkyl), N(C (1-4) alkyl) 2 , 4-hydroxy-piperidinyl, azetidin-1-yl, or fur-2-yl; provided that R 5 is not H if R 7 is OCH 3 ; R 6 is C (1-4) alkyl-Q, OC (1-4) alkyl-Q, C(O)NA 3 A 4 , C(O)OC (1-4) alkyl, O-tetrahydropyranyl, O—(N-methyl)-piperidinyl, cyclopropyl, cyclobutyl, pyrrolidinyl, cyclopentyl, tetrahydrofuranyl, cyclohexyl, 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, piperidinyl, or tetrahydropyranyl; wherein said cyclopropyl, cyclobutyl, cyclopentyl, pyrrolidinyl, piperidinyl, tetrahydropyranyl, and cyclohexyl are optionally substituted with F, C(O)C (1-3) alkyl, CO 2 C(CH 3 ) 3 , and C (1-4) alkyl, and up to one additional fluorine atom; provided that R 6 is not CH 2 -phenyl, CH 2 -pyridinyl, CH 2 -pyrimidinyl, CH 2 -pyrazinyl, nor CH 2 -pyridazyl; Q is H, CF 3 , OH, SO 2 CH 3 , —CN, NA 3 A 4 , OC (1-4) alkyl, cyclopropyl, cyclobutyl, oxetanyl, cyclopentyl, tetrahydrofuranyl, pyrazolyl, isoxazolyl, imidazolyl, triazolyl, oxazolyl, thiazolyl, pyrrolidinyl, cyclohexyl, piperidinyl, tetrahydropyranyl, 1,1-dioxo-tetrahydrothiopyran-4-yl, tetrahydrothiopyran-4-yl, phenyl, pyridinyl, pyrimidinyl, pyrazinyl, or pyridazyl; wherein said cyclopropyl, cyclobutyl, cyclopentyl, pyrrolidinyl, piperidinyl, tetrahydropyranyl, and cyclohexyl are optionally substituted with F, C(O)C (1-3) alkyl (including C(O)CH 3 ), CO 2 C(CH 3 ) 3 , and C (1-4) alkyl, and up to one additional fluorine atom; and said pyrazolyl, isoxazolyl, imidazolyl, triazolyl, oxazolyl, and thiazolyl are all optionally substituted with one or two CH 3 groups; and said oxetanyl is optionally substituted with CH 3 ;

wherein

A 3 is H, or C (1-4) alkyl; A 4 is H, C (1-4) alkyl, CH 2 -cyclopropyl, cyclopropyl, C (1-3) alkylCF 3 , CH 2 CH 2 OCH 2 CF 3 , C(O)C (1-2) alkylCF 3 ,

or C (0-1) alkyl-trifluoromethyl-cyclohexyl, or A 3 and A 4 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

›DETAILED DESCRIPTION OF THE INVENTION · 3 of 6

wherein

q b is H, F, CF 3 , SO 2 CH 3 , OC (1-4) alkyl, pyrazol-1-yl, or 3-trifluoromethyl-pyrazol-1-yl, imidazol-1-yl, or triazolyl; q c is H, F, CF 3 , OC (1-4) alkyl, or OH; q d is H, CH 2 CF 3 , C (1-4) alkyl, C(O)C (1-4) alkyl (including C(O)CH 3 ), or phenyl; provided that if R 6 is OCH 2 -Q, then Q may not be OH, nor NA 3 A 4 ; R 7 is H, Cl, —CN, C (1-4) alkyl, cyclopropyl, OC (1-4) alkylCF 3 , OCH 2 CH 2 OC (1-4) alkyl, CF 3 , SCH 3 , CH 2 NA 1 A 2 , CH 2 OC (2-3) alkylNA 1 A 2 , NA 1 A 2 , C(O)NA 1 A 2 , N(CH 3 )C (2-4) alkylNA 1 A 2 , OC (2-4) alkylNA 1 A 2 , OC (1-4) alkyl, OCH 2 -(1-methyl)-imidazol-2-yl, furyl, pyrazolyl, imidazolyl, pyridyl, pyridazyl, pyrazinyl, pyrimidinyl, thiophenyl, 1-methyl-indazolyl, phenyl, or

wherein said imidazolyl or pyrazolyl is optionally substituted with one CH 3 group;

R a is H, OC (1-4) alkyl, CH 2 OH, NH(CH 3 ), N(CH 3 ) 2 , NH 2 , CH 3 , F, or OH;

R b is H, CO 2 C(CH 3 ) 3 , C (1-4) alkyl, C(O)C (1-4) alkyl (including C(O)CH 3 ), SO 2 C (1-4) alkyl, CH 2 CH 2 CF 3 , CH 2 CF 3 , CH 2 -cyclopropyl, phenyl, CH 2 -phenyl, or C (3-6) cycloalkyl;

R 8 is H, CH 3 , OCH 3 , or F;

R 9 is H, or F;

and pharmaceutically acceptable salts thereof;

In another embodiment of the invention:

R 1 is pyrrolyl, pyrazolyl, imidazolyl, triazolyl, thiazolyl, pyridyl, pyridyl N-oxide, pyrazinyl, pyrimidinyl, pyridazyl, piperidinyl, tetrahydropyranyl, phenyl, oxazolyl, isoxazolyl, thiophenyl, benzoxazolyl, or quinolinyl; wherein said piperidinyl, pyridyl, pyridyl N-oxide, imidazolyl, phenyl, thiophenyl, benzoxazolyl, and pyrazolyl are optionally substituted with C(O)C (1-4) alkyl (including C(O)CH 3 ), C(O)NH 2 , C (1-4) alkyl (including CH 3 , and CH 2 CH 3 ), CF 3 , CH 2 CF 3 , Cl, F, —CN, OC (1-4) alkyl (including OCH 3 ), N(C (1-4) alkyl) 2 (including N(CH 3 ) 2 ), —(CH 2 ) 3 OCH 3 , SC (1-4) alkyl (including SCH 3 ), OH, CO 2 H, CO 2 C (1-4) alkyl (including CO 2 C(CH 3 ) 3 ), OCF 3 , OCHF 2 , SO 2 CH 3 , SO 2 NH 2 , or OCH 2 OCH 3 ; and optionally substituted with up to two additional substituents independently selected from the group consisting of Cl, OCH 3 , and CH 3 ; and wherein said triazolyl, oxazolyl, isoxazolyl, and thiazolyl are optionally substituted with one or two CH 3 groups; R 2 is H, C (1-4) alkyl, —C≡CH, 1-methyl-triazolyl, pyridyl, pyridyl-N-oxide, 1-methyl-pyrazolyl, pyrimidinyl, pyrazinyl, oxazolyl, isoxazolyl, N-acetyl-azetidin-3-yl, N-methylsulfonyl-azetidin-3-yl, N-Boc-azetidin-3-yl, N-acetyl piperidinyl, 1-H-piperidinyl, N-Boc-piperidinyl, N—C (1-2) alkyl-piperidinyl, N-methylsulfonyl-piperidinyl, thiazolyl, pyridazyl, 1-(3-methoxypropyl)-imidazolyl, or 1-C (1-2) alkyl-imidazolyl; wherein said 1-C (1-2) alkyl imidazolyl is optionally substituted with up to two additional CH 3 groups, or one substituent selected from the group consisting of SCH 3 , and Cl; and said pyridyl, and pyridyl-N-oxide are optionally substituted with up to two substitutents independently selected from the group consisting of SO 2 CH 3 , SO 2 NH 2 , C(O)NH 2 , —CN, OCH 3 , CF 3 , Cl, and CH 3 ; and said thiazolyl, oxazolyl and isoxazolyl are optionally substituted with up to two CH 3 groups; and said 1-methyl pyrazolyl is optionally substituted with up to two additional CH 3 groups; R 3 is H, OH, OCH 3 , or NH 2 ; R 4 is H, or F; R 5 is H, Cl, —CN, CF 3 , SC (1-4) alkyl (including SCH 3 ), OC (1-4) alkyl (including OC (1-3) alkyl) OH, C (1-4) alkyl, N(CH 3 )OCH 3 , NH(C (1-4) alkyl) (including NH(C (1-2) alkyl)), N(C (1-4) alkyl) 2 (including N(C (1-2) alkyl) 2 ), 4-hydroxy-piperidinyl, azetidin-1-yl, or fur-2-yl; provided that R 5 is not H if R 7 is OCH 3 ; R 6 is C (1-4) alkyl-Q, OC (1-4) alkyl-Q, C(O)NA 3 A 4 , C(O)OC (1-4) alkyl, O-tetrahydropyranyl, O—(N-methyl)-piperidinyl, cyclopropyl, cyclobutyl, pyrrolidinyl, cyclopentyl, tetrahydrofuranyl, cyclohexyl, 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, piperidinyl, or tetrahydropyranyl; wherein said cyclopropyl, cyclobutyl, cyclopentyl, pyrrolidinyl, piperidinyl, tetrahydropyranyl, and cyclohexyl are optionally substituted with F, CO 2 C(CH 3 ) 3 , and C (1-4) alkyl (including CH 3 ), and up to one additional fluorine atom; provided that R 6 is not CH 2 -phenyl, CH 2 -pyridinyl, nor CH 2 -pyrimidinyl; Q is H, CF 3 , OH, SO 2 CH 3 , —CN, NA 3 A 4 , OC (1-4) alkyl, cyclopropyl, cyclobutyl, oxetanyl, cyclopentyl, tetrahydrofuranyl, pyrazolyl, isoxazolyl, imidazolyl, triazolyl, oxazolyl, thiazolyl, pyrrolidinyl, cyclohexyl, piperidinyl, tetrahydropyranyl, 1,1-dioxo-tetrahydrothiopyran-4-yl, tetrahydrothiopyran-4-yl, phenyl, pyridinyl, or pyrimidinyl; wherein said cyclopropyl, cyclobutyl, cyclopentyl, pyrrolidinyl, piperidinyl, tetrahydropyranyl, and cyclohexyl are optionally substituted with F, C(O)CH 3 , CO 2 C(CH 3 ) 3 , and C (1-4) alkyl (including CH 3 ), and up to one additional fluorine atom; and said pyrazolyl, isoxazolyl, imidazolyl, triazolyl, oxazolyl, and thiazolyl are all optionally substituted with one or two CH 3 groups; and said oxetanyl is optionally substituted with CH 3 ;

wherein

A 3 is H, or C (1-4) alkyl; A 4 is H, C (1-4) alkyl, CH 2 -cyclopropyl, cyclopropyl, C (1-3) alkylCF 3 , CH 2 CH 2 OCH 2 CF 3 , C(O)C (1-2) alkylCF 3 ,

or C (0-1) alkyl-trifluoromethyl-cyclohexyl, or A 3 and A 4 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

wherein

q b is H, F, CF 3 , SO 2 CH 3 , OC (1-4) alkyl (including OCH 3 ), pyrazol-1-yl, 3-trifluoromethyl-pyrazol-1-yl, imidazol-1-yl, or triazolyl; q c is H, F, CF 3 , OC (1-4) alkyl, or OH; q d is H, CH 2 CF 3 , C (1-4) alkyl, C(O)CH 3 , or phenyl; provided that if R 6 is OCH 2 -Q, then Q may not be OH, nor NA 3 A 4 ; R 7 is H, Cl, —CN, C (1-4) alkyl, cyclopropyl, OC (1-4) alkylCF 3 (including OCH 2 CF 3 ), OCH 2 CH 2 OC (1-4) alkyl (including OCH 2 CH 2 OCH 3 ), CF 3 , SCH 3 , NA 1 A 2 , C(O)NA 1 A 2 (including C(O)NHCH 3 ), N(CH 3 )C (2-4) alkylNA 1 A 2 (including N(CH 3 )CH 2 CH 2 NA 1 A 2 ), OC (2-4) alkylNA 1 A 2 (including OCH 2 CH 2 NA 1 A 2 ), OC (1-4) alkyl (including OC (1-3) alkyl), OCH 2 -(1-methyl)-imidazol-2-yl, imidazolyl, furyl, pyrazolyl, pyridyl, pyrimidinyl, thiophenyl, 1-methyl-indazolyl, phenyl, or

›DETAILED DESCRIPTION OF THE INVENTION · 4 of 6

wherein said imidazolyl or pyrazolyl is optionally substituted with one CH 3 group;

A 1 is H, or C (1-4) alkyl; A 2 is H, C (1-4) alkyl, C (1-4) alkylOC (1-4) alkyl, C (1-4) alkylOH, C(O)C (1-4) alkyl (including C(O)C (1-2) alkyl), or OC (1-4) alkyl (including OCH 3 ); or A 1 and A 2 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

R a is H, F, OC (1-4) alkyl (including OCH 3 ), or OH;

R b is C (1-4) alkyl (including CH 3 ), C(O)CH 3 , or phenyl;

R 8 is H, CH 3 , OCH 3 , or F;

R 9 is H, or F;

and pharmaceutically acceptable salts thereof;

In another embodiment of the invention:

R 1 is pyrrolyl, pyrazolyl, imidazolyl, triazolyl, thiazolyl, pyridyl, pyridyl N-oxide, pyrazinyl, pyrimidinyl, pyridazyl, piperidinyl, tetrahydropyranyl, phenyl, oxazolyl, isoxazolyl, thiophenyl, benzoxazolyl, or quinolinyl; wherein said piperidinyl, pyridyl, pyridyl N-oxide, imidazolyl, phenyl, thiophenyl, benzoxazolyl, and pyrazolyl are optionally substituted with SO 2 CH 3 , C(O)CH 3 , C(O)NH 2 , CH 3 , CH 2 CH 3 , CF 3 , Cl, F, —CN, OCH 3 , N(CH 3 ) 2 , —(CH 2 ) 3 OCH 3 , SCH 3 , OH, CO 2 H, CO 2 C(CH 3 ) 3 , or OCH 2 OCH 3 ; and optionally substituted with up to two additional substituents independently selected from the group consisting of Cl, OCH 3 , and CH 3 ; and wherein said triazolyl, oxazolyl, isoxazolyl, and thiazolyl are optionally substituted with one or two CH 3 groups; R 2 is H, C (1-4) alkyl (including CH 3 ), —C≡CH, 1-methyl-1,2,3-triazolyl, pyridyl, pyridyl-N-oxide, 1-methyl-pyrazol-4-yl, pyrimidin-5-yl, pyridazyl, pyrazin-2-yl, isoxazolyl, N-acetyl-azetidin-3-yl, N-methylsulfonyl-azetidin-3-yl, N-Boc-azetidin-3-yl, N-acetyl piperidinyl, N-methylsulfonyl-piperidinyl, 1-H-piperidinyl, N-Boc-piperidinyl, N—C (1-2) alkyl-piperidinyl, thiazol-5-yl, 1-(3-methoxypropyl)-imidazol-5-yl, or 1-C (1-2) alkyl imidazol-5-yl (including 1-ethyl imidazol-5-yl and 1-methyl imidazol-5-yl); wherein said 1-C (1-2) alkyl-imidazol-5-yl (including 1-methyl imidazol-5-yl) is optionally substituted with up to two additional CH 3 groups, or one substituent selected from the group consisting of SCH 3 , and Cl; and said pyridyl, and pyridyl-N-oxide are optionally substituted with up to two substituents independently selected from the group consisting of C(O)NH 2 , —CN, OCH 3 , CF 3 , Cl, and CH 3 ; and said thiazol-5-yl, and said isoxazolyl are optionally substituted with up to two CH 3 groups; and said 1-methyl pyrazol-4-yl is optionally substituted with up to two additional CH 3 groups; R 3 is H, OH, OCH 3 , or NH 2 ; R 4 is H, or F; R 5 is H, Cl, —CN, CF 3 , SCH 3 , OC (1-3) alkyl, OH, C (1-4) alkyl (including C (1-2) alkyl), N(CH 3 )OCH 3 , NH(C (1-2) alkyl), N(C (1-2) alkyl) 2 , 4-hydroxy-piperidinyl, azetidin-1-yl, or fur-2-yl; provided that R 5 is not H if R 7 is OCH 3 ; R 6 is C (1-4) alkyl-Q, OC (1-4) alkyl-Q, C(O)NA 3 A 4 , C(O)OC (1-4) alkyl, O-tetrahydropyranyl, O—(N-methyl)-piperidinyl, cyclopropyl, cyclobutyl, pyrrolidinyl, cyclopentyl, tetrahydrofuranyl, cyclohexyl, 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, piperidinyl, or tetrahydropyran-4-yl; wherein said cyclopropyl, cyclobutyl, cyclopentyl, pyrrolidinyl, piperidinyl, and cyclohexyl are optionally substituted with F, CO 2 C(CH 3 ) 3 , and CH 3 , and up to one additional fluorine atom; provided that R 6 is not CH 2 -phenyl, CH 2 -pyridinyl, nor CH 2 -pyrimidinyl; Q is H, CF 3 , OH, SO 2 CH 3 , —CN, NA 3 A 4 , OC (1-4) alkyl, cyclopropyl, cyclobutyl, oxetanyl, cyclopentyl, tetrahydrofuranyl, 1,3-dimethyl-pyrazol-5-yl, 3,5-dimethyl-isoxazol-4-yl, thiazol-2-yl, pyrrolidinyl, cyclohexyl, piperidinyl, tetrahydropyran-4-yl, 1,1-dioxo-tetrahydrothiopyran-4-yl, tetrahydrothiopyran-4-yl, phenyl, pyridin-3-yl, or pyrimidin-2-yl; wherein said cyclopropyl, cyclobutyl, cyclopentyl, pyrrolidinyl, piperidinyl, and cyclohexyl are optionally substituted with F, C(O)CH 3 , CO 2 C(CH 3 ) 3 , and CH 3 , and up to one additional fluorine atom; and said oxetanyl is optionally substituted with CH 3 ;

wherein

A 3 is H, or C (1-4) alkyl (including CH 3 ); A 4 is H, C (1-4) alkyl (including CH 3 ), CH 2 -cyclopropyl, cyclopropyl, C (1-3) alkylCF 3 , CH 2 CH 2 OCH 2 CF 3 , C(O)C (1-2) alkylCF 3 ,

or C (0-1) alkyl-trifluoromethyl-cyclohexyl, or

A 3 and A 4 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

wherein

q b is H, F, CF 3 , SO 2 CH 3 , OCH 3 , pyrazol-1-yl, 3-trifluoromethyl-pyrazol-1-yl, or imidazol-1-yl; q c is H, F, or CF 3 , q d is CH 2 CF 3 ; provided that if R 6 is OCH 2 -Q, then Q may not be OH, nor NA 3 A 4 ; R 7 is H, Cl, —CN, C (1-4) alkyl, cyclopropyl, OCH 2 CF 3 , OCH 2 CH 2 OCH 3 , CF 3 , SCH 3 , NA 1 A 2 , C(O)NHCH 3 , N(CH 3 )CH 2 CH 2 NA 1 A 2 , OCH 2 CH 2 NA 1 A 2 , OC (1-3) alkyl (including OC (1-2) alkyl), OCH 2 -(1-methyl)-imidazol-2-yl, imidazol-2-yl, pyrazol-4-yl, pyrid-3-yl, pyrimidin-5-yl, thiophen-3-yl, 1-methyl-indazol-5-yl, 1-methyl-indazol-6-yl, fur-2-yl, phenyl, or

wherein said imidazol-2-yl or pyrazol-4-yl are optionally substituted with one CH 3 group;

A 1 is H, or C (1-4) alkyl (including C (1-2) alkyl); A 2 is H, C (1-4) alkyl (including C (1-2) alkyl), C (1-4) alkylOC (1-4) alkyl (including CH 2 CH 2 OCH 3 ), C (1-4) alkylOH, C(O)C (1-2) alkyl, or OCH 3 ; or A 1 and A 2 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

R a is H, F, OCH 3 , or OH;

R b is CH 3 , or phenyl;

R 8 is H, CH 3 , OCH 3 , or F;

R 9 is H, or F;

and pharmaceutically acceptable salts thereof;

In another embodiment of the invention

R 1 is imidazolyl, pyrimidinyl, triazolyl, tetrahydropyranyl, thiazolyl, pyridyl, piperidinyl, phenyl, or oxazolyl; wherein said piperidinyl, pyridyl, imidazolyl, and phenyl are optionally substituted with SO 2 CH 3 , C(O)CH 3 , CH 3 , CF 3 , Cl, F, —CN, OCH 3 , or N(CH 3 ) 2 ; and optionally substituted with up to one additional group independently selected from the group consisting of Cl, OCH 3 , and CH 3 ; and wherein said triazolyl, oxazolyl, and thiazolyl are optionally substituted with one or two CH 3 groups; R 2 is H, CH 3 , —C≡CH, 1-methyl-1,2,3-triazol-5-yl, pyrid-3-yl, 2-trifluoromethyl-pyrid-4-yl, 1-methyl-pyrazol-4-yl, 1,3,5-trimethyl-pyrazol-4-yl, thiazol-5-yl, N-acetyl-azetidin-3-yl, N-methylsulfonyl-azetidin-3-yl, N-Boc-azetidin-3-yl, N-acetyl-piperidin-4-yl, N-Boc-piperidin-4-yl, 1-H-piperidin-4-yl, N-methylsulfonyl-piperidin-4-yl, 1,2-dimethyl-imidazol-5-yl, or 1-methyl-imidazol-5-yl; R 3 is OH; R 4 is H; R 5 is H, Cl, —CN, CF 3 , C (1-2) alkyl, OH, N(CH 3 )OCH 3 , OCH 3 , azetidin-1-yl, or fur-2-yl; provided that R 5 is not H if R 7 is OCH 3 ; R 6 is C (1-4) alkyl-Q, OC (1-4) alkyl-Q, C(O)NA 3 A 4 , C(O)OC (1-4) alkyl, O-tetrahydropyranyl, O—(N-methyl)-piperidinyl, cyclopentyl, cyclohexyl, 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, or tetrahydropyran-4-yl; provided that R 6 is not CH 2 -phenyl, CH 2 -pyridinyl, nor CH 2 -pyrimidinyl; Q is H, CF 3 , OH, SO 2 CH 3 , NA 3 A 4 , OC (1-4) alkyl, cyclopropyl, 1-methyl-cyclopropyl, oxetanyl, 3-methyl-oxetanyl, tetrahydrofuranyl, 1,3-dimethyl-pyrazol-5-yl, 3,5-dimethyl-isoxazol-4-yl, thiazol-2-yl, N-methyl-pyrrolidin-2-yl, cyclohexyl, N-acetyl-piperidin-4-yl, N-Boc-piperidin-4-yl, 1-H-piperidin-4-yl, tetrahydropyran-4-yl, 1,1-dioxo-tetrahydrothiopyran-4-yl, tetrahydrothiopyran-4-yl, phenyl, pyridin-3-yl, or pyrimidin-2-yl; wherein said cyclopropyl, and said cyclohexyl are optionally substituted with up to two fluorine atoms;

›DETAILED DESCRIPTION OF THE INVENTION · 5 of 6

wherein

A 3 is H, or CH 3 ; A 4 is CH 3 , CH 2 -cyclopropyl, cyclopropyl, C (1-3) alkylCF 3 , CH 2 CH 2 OCH 2 CF 3 , C(O)C (1-2) alkylCF 3 ,

or C (0-1) alkyl-trifluoromethyl-cyclohexyl, or A 3 and A 4 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

wherein

q b is H, F, CF 3 , SO 2 CH 3 , pyrazol-1-yl, or 3-trifluoromethyl-pyrazol-1-yl; q c is H, F, or CF 3 , q d is CH 2 CF 3 ;

provided that if R 6 is OCH 2 -Q, then Q may not be OH, nor NA 3 A 4 ;

R 7 is Cl, —CN, CF 3 , C (1-4) alkyl, cyclopropyl, NA 1 A 2 , C(O)NHCH 3 , OCH 2 CH 2 OCH 3 , 1-methyl imidazol-2-yl, 1-methyl pyrazol-4-yl, OC (1-2) alkyl (including OCH 3 ), pyrimidin-5-yl, thiophen-3-yl, 1-methyl-indazol-5-yl, 1-methyl-indazol-6-yl, fur-2-yl, phenyl, or

and pharmaceutically acceptable salts thereof.

In another embodiment of the invention

R 1 is imidazolyl, triazolyl, tetrahydropyranyl, thiazolyl, pyridyl, or phenyl; wherein said pyridyl, imidazolyl, and phenyl are optionally substituted with one substituent selected from the group consisting of CH 3 , CF 3 , Cl, and —CN; and optionally substituted with up to one additional CH 3 ; and wherein said triazolyl, and thiazolyl are optionally substituted with one or two CH 3 groups; R 2 is H, CH 3 , 1-methyl-1,2,3-triazol-5-yl, pyrid-3-yl, 2-trifluoromethyl-pyrid-4-yl, 1,3,5-trimethyl-pyrazol-4-yl, N-acetyl-azetidin-3-yl, N-methylsulfonyl-azetidin-3-yl, N-Boc-azetidin-3-yl, N-acetyl-piperidin-4-yl, N-Boc-piperidin-4-yl, 1-H-piperidin-4-yl, N-methylsulfonyl-piperidin-4-yl, 1,2-dimethyl-imidazol-5-yl, or 1-methyl-imidazol-5-yl; R 3 is OH; R 4 is H; R 5 is H, Cl, —CN, CF 3 , C (1-2) alkyl, OCH 3 , azetidin-1-yl, or fur-2-yl; provided that R 5 is not H if R 7 is OCH 3 ; R 6 is C (1-4) alkyl-Q, OC (1-4) alkyl-Q, C(O)NA 3 A 4 , C(O)OC (1-4) alkyl, O-tetrahydropyranyl, O—(N-methyl)-piperidinyl, cyclopentyl, cyclohexyl, 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, or tetrahydropyran-4-yl; provided that R 6 is not CH 2 -phenyl, CH 2 -pyridinyl, nor CH 2 -pyrimidinyl; Q is H, CF 3 , OH, SO 2 CH 3 , NA 3 A 4 , OC (1-4) alkyl, cyclopropyl, 1-methyl-cyclopropyl, oxetanyl, 3-methyl-oxetanyl, tetrahydrofuranyl, 1,3-dimethyl-pyrazol-5-yl, 3,5-dimethyl-isoxazol-4-yl, thiazol-2-yl, N-methyl-pyrrolidin-2-yl, cyclohexyl, N-acetyl-piperidin-4-yl, N-Boc-piperidin-4-yl, 1-H-piperidin-4-yl, tetrahydropyran-4-yl, 1,1-dioxo-tetrahydrothiopyran-4-yl, tetrahydrothiopyran-4-yl, phenyl, pyridin-3-yl, or pyrimidin-2-yl; wherein said cyclopropyl, and said cyclohexyl are optionally substituted with up to two fluorine atoms;

wherein

A 3 is H, or CH 3 ; A 4 is CH 3 , CH 2 -cyclopropyl, cyclopropyl, C (1-3) alkylCF 3 , CH 2 CH 2 OCH 2 CF 3 , C(O)C (1-2 alkylCF 3 ,

C (0-1) alkyl-trifluoromethyl-cyclohexyl, or A 3 and A 4 may be taken together with their attached nitrogen to form a ring selected from the group consisting of:

wherein

q b is H, F, CF 3 , SO 2 CH 3 , pyrazol-1-yl, or 3-trifluoromethyl-pyrazol-1-yl; q c is H, F, or CF 3 , q d is CH 2 CF 3 ;

provided that if R 6 is OCH 2 -Q, then Q may not be OH, nor NA 3 A 4 ;

R 7 is Cl, CF 3 , CH 2 CH 3 , cyclopropyl, OCH 3 , pyrimidin-5-yl, thiophen-3-yl, 1-methyl-indazol-5-yl, 1-methyl-indazol-6-yl, fur-2-yl, azetidin-1-yl, phenyl, or

R 8 is H, or CH 3 ;

R 9 is H;

and pharmaceutically acceptable salts thereof.

Another embodiment of the invention is a compound selected from the group consisting of:

and pharmaceutically acceptable salts thereof.

Another embodiment of the invention comprises a compound of Formula I and a pharmaceutically acceptable carrier.

The present invention also provides a method for preventing, treating or ameliorating an RORγt mediated inflammatory syndrome, disorder or disease comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of preventing, treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is selected from the group consisting of: ophthalmic disorders, uveitis, atherosclerosis, rheumatoid arthritis, psoriasis, psoriatic arthritis, atopic dermatitis, multiple sclerosis, Crohn's Disease, ulcerative colitis, ankylosing spondylitis, nephritis, organ allograft rejection, fibroid lung, systic fibrosis, renal insufficiency, diabetes and diabetic complications, diabetic nephropathy, diabetic retinopathy, diabetic retinitis, diabetic microangiopathy, tuberculosis, chronic obstructive pulmonary disease, sarcoidosis, invasive staphylococcia, inflammation after cataract surgery, allergic rhinitis, allergic conjunctivitis, chronic urticaria, systemic lupus erythematosus, asthma, allergic asthma, steroid resistant asthma, neutrophilic asthma, periodontal diseases, periodonitis, gingivitis, gum disease, diastolic cardiomyopathies, cardiac infarction, myocarditis, chronic heart failure, angiostenosis, restenosis, reperfusion disorders, glomerulonephritis, solid tumors and cancers, chronic lymphocytic leukemia, chronic myelocytic leukemia, multiple myeloma, malignant myeloma, Hodgkin's disease, and carcinomas of the bladder, breast, cervix, colon, lung, prostate, or stomach comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is selected from the group consisting of: rheumatoid arthritis, psoriasis, chronic obstructive pulmonary disorder, psoriatic arthritis, ankylosing spondylitis, Crohn's disease, and ulcerative colitis comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

›DETAILED DESCRIPTION OF THE INVENTION · 6 of 6

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is selected from the group consisting of: inflammatory bowel diseases, rheumatoid arthritis, psoriasis, chronic obstructive pulmonary disorder, psoriatic arthritis, ankylosing spondylitis, neutrophilic asthma, steroid resistant asthma, multiple sclerosis, and systemic lupus erythematosus comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is selected from the group consisting of: rheumatoid arthritis, and psoriasis comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, in a subject in need thereof comprising administering to the subject an effective amount of the compound of Formula I or composition or medicament thereof in a combination therapy with one or more anti-inflammatory agents, or immunosuppressive agents, wherein said syndrome, disorder or disease is selected from the group consisting of: rheumatoid arthritis, and psoriasis.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is rheumatoid arthritis, comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is psoriasis comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is chronic obstructive pulmonary disorder comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is psoriatic arthritis comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is ankylosing spondylitis comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating an inflammatory bowel disease, wherein said inflammatory bowel disease is Crohn's disease comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating an inflammatory bowel disease, wherein said inflammatory bowel disease is ulcerative colitis comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is neutrophilic asthma comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is steroid resistant asthma comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is multiple sclerosis comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The present invention provides a method of treating or ameliorating a syndrome, disorder or disease, wherein said syndrome, disorder or disease is systemic lupus erythematosus comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

The invention also relates to methods of modulating RORγt activity in a mammal by administration of an effective amount of at least one compound of Formula I.

›Definitions · 1 of 3

The term “administering” with respect to the methods of the invention, means a method for therapeutically or prophylactically preventing, treating or ameliorating a syndrome, disorder or disease as described herein by using a compound of Formula I or a form, composition or medicament thereof. Such methods include administering an effective amount of said compound, compound form, composition or medicament at different times during the course of a therapy or concurrently in a combination form. The methods of the invention are to be understood as embracing all known therapeutic treatment regimens.

The term “subject” refers to a patient, which may be an animal, typically a mammal, typically a human, which has been the object of treatment, observation or experiment and is at risk of (or susceptible to) developing a syndrome, disorder or disease that is associated with abberant RORγt expression or RORγt overexpression, or a patient with an inflammatory condition that accompanies syndromes, disorders or diseases associated with abberant RORγt expression or RORγt overexpression.

The term “effective amount” means that amount of active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue system, animal or human, that is being sought by a researcher, veterinarian, medical doctor, or other clinician, which includes preventing, treating or ameliorating the symptoms of a syndrome, disorder or disease being treated.

As used herein, the term “composition” is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combinations of the specified ingredients in the specified amounts.

The term “alkyl” refers to both linear and branched chain radicals of up to 12 carbon atoms, preferably up to 6 carbon atoms, unless otherwise indicated, and includes, but is not limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, hexyl, isohexyl, heptyl, octyl, 2,2,4-trimethylpentyl, nonyl, decyl, undecyl and dodecyl. Any alkyl group may be optionally substituted with one OCH 3 , one OH, or up to two fluorine atoms.

The term “C (a-b) ” (where a and b are integers referring to a designated number of carbon atoms) refers to an alkyl, alkenyl, alkynyl, alkoxy or cycloalkyl radical or to the alkyl portion of a radical in which alkyl appears as the prefix root containing from a to b carbon atoms inclusive. For example, C (1-4) denotes a radical containing 1, 2, 3 or 4 carbon atoms.

The term “cycloalkyl” refers to a saturated or partially unsaturated monocyclic or bicyclic hydrocarbon ring radical derived by the removal of one hydrogen atom from a single ring carbon atom. Typical cycloalkyl radicals include cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptyl and cyclooctyl. Additional examples include C (3-6) cycloalkyl, C (5-8) cycloalkyl, decahydronaphthalenyl, and 2,3,4,5,6,7-hexahydro-1H-indenyl. Any cycloalkyl group may be optionally substituted with one OCH 3 , one OH, or up to two fluorine atoms.

As used herein, the term “thiophenyl” is intended to describe the radical formed by removing a hydrogen atom from the molecule with the structure:

Pharmaceutically Acceptable Salts

Pharmaceutically acceptable acidic/anionic salts include, and are not limited to acetate, benzenesulfonate, benzoate, bicarbonate, bitartrate, bromide, calcium edetate, camsylate, carbonate, chloride, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, glyceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, iodide, isethionate, lactate, lactobionate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate, mucate, napsylate, nitrate, pamoate, pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, subacetate, succinate, sulfate, tannate, tartrate, teoclate, tosylate and triethiodide. Organic or inorganic acids also include, and are not limited to, hydriodic, perchloric, sulfuric, phosphoric, propionic, glycolic, methanesulfonic, hydroxyethanesulfonic, oxalic, 2-naphthalenesulfonic, p-toluenesulfonic, cyclohexanesulfamic, saccharinic or trifluoroacetic acid.

Pharmaceutically acceptable basic/cationic salts include, and are not limited to aluminum, 2-amino-2-hydroxymethyl-propane-1,3-diol (also known as tris(hydroxymethyl)aminomethane, tromethane or “TRIS”), ammonia, benzathine, t-butylamine, calcium, calcium gluconate, calcium hydroxide, chloroprocaine, choline, choline bicarbonate, choline chloride, cyclohexylamine, diethanolamine, ethylenediamine, lithium, LiOMe, L-lysine, magnesium, meglumine, NH 3 , NH 4 OH, N-methyl-D-glucamine, piperidine, potassium, potassium-t-butoxide, potassium hydroxide (aqueous), procaine, quinine, sodium, sodium carbonate, sodium-2-ethylhexanoate, sodium hydroxide, triethanolamine, or zinc.

Methods of Use

The present invention is directed to a method for preventing, treating or ameliorating a RORγt mediated inflammatory syndrome, disorder or disease comprising administering to a subject in need thereof an effective amount of a compound of Formula I or a form, composition or medicament thereof.

Since RORγt is an N-terminal isoform of RORγ, it is recognized that compounds of the present invention which are modulators of RORγt are likely to be modulators of RORγ as well. Therefore the mechanistic description “RORγt modulators” is intended to encompass RORγ modulators as well.

When employed as RORγt modulators, the compounds of the invention may be administered in an effective amount within the dosage range of about 0.5 mg to about 10 g, preferably between about 0.5 mg to about 5 g, in single or divided daily doses. The dosage administered will be affected by factors such as the route of administration, the health, weight and age of the recipient, the frequency of the treatment and the presence of concurrent and unrelated treatments.

›Definitions · 2 of 3

It is also apparent to one skilled in the art that the therapeutically effective dose for compounds of the present invention or a pharmaceutical composition thereof will vary according to the desired effect. Therefore, optimal dosages to be administered may be readily determined by one skilled in the art and will vary with the particular compound used, the mode of administration, the strength of the preparation, and the advancement of the disease condition. In addition, factors associated with the particular subject being treated, including subject age, weight, diet and time of administration, will result in the need to adjust the dose to an appropriate therapeutic level. The above dosages are thus exemplary of the average case. There can, of course, be individual instances where higher or lower dosage ranges are merited, and such are within the scope of this invention.

The compounds of Formula I may be formulated into pharmaceutical compositions comprising any known pharmaceutically acceptable carriers. Exemplary carriers include, but are not limited to, any suitable solvents, dispersion media, coatings, antibacterial and antifungal agents and isotonic agents. Exemplary excipients that may also be components of the formulation include fillers, binders, disintegrating agents and lubricants.

The pharmaceutically-acceptable salts of the compounds of Formula I include the conventional non-toxic salts or the quaternary ammonium salts which are formed from inorganic or organic acids or bases. Examples of such acid addition salts include acetate, adipate, benzoate, benzenesulfonate, citrate, camphorate, dodecylsulfate, hydrochloride, hydrobromide, lactate, maleate, methanesulfonate, nitrate, oxalate, pivalate, propionate, succinate, sulfate and tartrate.

Base salts include ammonium salts, alkali metal salts such as sodium and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases such as dicyclohexylamino salts and salts with amino acids such as arginine. Also, the basic nitrogen-containing groups may be quaternized with, for example, alkyl halides.

The pharmaceutical compositions of the invention may be administered by any means that accomplish their intended purpose. Examples include administration by parenteral, subcutaneous, intravenous, intramuscular, intraperitoneal, transdermal, buccal or ocular routes. Alternatively or concurrently, administration may be by the oral route. Suitable formulations for parenteral administration include aqueous solutions of the active compounds in water-soluble form, for example, water-soluble salts, acidic solutions, alkaline solutions, dextrose-water solutions, isotonic carbohydrate solutions and cyclodextrin inclusion complexes.

The present invention also encompasses a method of making a pharmaceutical composition comprising mixing a pharmaceutically acceptable carrier with any of the compounds of the present invention. Additionally, the present invention includes pharmaceutical compositions made by mixing a pharmaceutically acceptable carrier with any of the compounds of the present invention.

Polymorphs and Solvates

Furthermore, the compounds of the present invention may have one or more polymorph or amorphous crystalline forms and as such are intended to be included in the scope of the invention. In addition, the compounds may form solvates, for example with water (i.e., hydrates) or common organic solvents. As used herein, the term “solvate” means a physical association of the compounds of the present invention with one or more solvent molecules. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances the solvate will be capable of isolation, for example when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. The term “solvate” is intended to encompass both solution-phase and isolatable solvates. Non-limiting examples of suitable solvates include ethanolates, methanolates, and the like.

It is intended that the present invention include within its scope polymorphs and solvates of the compounds of the present invention. Thus, in the methods of treatment of the present invention, the term “administering” shall encompass the means for treating, ameliorating or preventing a syndrome, disorder or disease described herein with the compounds of the present invention or a polymorph or solvate thereof, which would obviously be included within the scope of the invention albeit not specifically disclosed.

In another embodiment, the invention relates to a compound as described in Formula I for use as a medicament.

In another embodiment, the invention relates to the use of a compound as described in Formula I for the preparation of a medicament for the treatment of a disease associated with an elevated or aberrant RORγt activity.

The present invention includes within its scope prodrugs of the compounds of this invention. In general, such prodrugs will be functional derivatives of the compounds which are readily convertible in vivo into the required compound. Thus, in the methods of treatment of the present invention, the term “administering” shall encompass the treatment of the various disorders described with the compound specifically disclosed or with a compound which may not be specifically disclosed, but which converts to the specified compound in vivo after administration to the patient. Conventional procedures for the selection and preparation of suitable prodrug derivatives are described, for example, in “Design of Prodrugs”, Ed. H. Bundgaard, Elsevier, 1985.

Furthermore, it is intended that within the scope of the present invention, any element, in particular when mentioned in relation to a compound of Formula I, shall comprise all isotopes and isotopic mixtures of said element, either naturally occurring or synthetically produced, either with natural abundance or in an isotopically enriched form. For example, a reference to hydrogen includes within its scope 1 H, 2 H (D), and 3 H (T). Similarly, references to carbon and oxygen include within their scope respectively 12 C, 13 C and 14 C and 16 O and 18 O. The isotopes may be radioactive or non-radioactive. Radiolabelled compounds of Formula I may comprise a radioactive isotope selected from the group of 3 H, 11 C, 18 F, 122 I, 123 I, 125 I, 131 I, 75 Br, 76 Br, 77 Br and 82 Br. Preferably, the radioactive isotope is selected from the group of 3 H, 11 C and 18 F.

›Definitions · 3 of 3

Some compounds of the present invention may exist as atropisomers. Atropisomers are stereoisomers resulting from hindered rotation about single bonds where the steric strain barrier to rotation is high enough to allow for the isolation of the conformers. It is to be understood that all such conformers and mixtures thereof are encompassed within the scope of the present invention.

Where the compounds according to this invention have at least one stereo center, they may accordingly exist as enantiomers or diastereomers. It is to be understood that all such isomers and mixtures thereof are encompassed within the scope of the present invention.

Where the processes for the preparation of the compounds according to the invention give rise to mixture of stereoisomers, these isomers may be separated by conventional techniques such as preparative chromatography. The compounds may be prepared in racemic form, or individual enantiomers may be prepared either by enantiospecific synthesis or by resolution. The compounds may, for example, be resolved into their component enantiomers by standard techniques, such as the formation of diastereomeric pairs by salt formation with an optically active acid, such as (−)-di-p-toluoyl-D-tartaric acid and/or (+)-di-p-toluoyl-L-tartaric acid followed by fractional crystallization and regeneration of the free base. The compounds may also be resolved by formation of diastereomeric esters or amides, followed by chromatographic separation and removal of the chiral auxiliary. Alternatively, the compounds may be resolved using a chiral HPLC column.

During any of the processes for preparation of the compounds of the present invention, it may be necessary and/or desirable to protect sensitive or reactive groups on any of the molecules concerned. This may be achieved by means of conventional protecting groups, such as those described in Protective Groups in Organic Chemistry , ed. J. F. W. McOmie, Plenum Press, 1973; and T. W. Greene & P. G. M. Wuts, Protective Groups in Organic Synthesis , John Wiley & Sons, 1991. The protecting groups may be removed at a convenient subsequent stage using methods known from the art.

›Abbreviations · 1 of 5

Herein and throughout the application, the following abbreviations may be used.

Å angstrom Ac acetyl ACN acetonitrile Ac 2 O acetic anhydride AIBN 2,2′-azobis(2-methylpropionitrile) Boc tert-butyloxy carbonyl BHT butylated hydroxytoluene Bn benzyl br broad Bu butyl n-BuLi n-butyl lithium d doublet dba dibenzylideneacetone CDI 1,1′-carbonyldiimidazole DCC dicyclohexylcarbodiimide DCE 1,2-dichloroethane DCM dichloromethane Dess-Martin periodinane 1,1,1-tris(acetyloxy)-1,1-dihydro-1,2-benziodoxol-3-(1H)-one DIAD diisopropyl azodicarboxylate DIPEA diisopropylethyl amine DMA dimethylacetamide DMF N,N-dimethylformamide DMSO dimethyl sulfoxide DPPA diphenyl phosphoryl azide dppf (diphenylphosphino)ferrocene Eaton's Reagent 7.7 wt % phosphorus pentoxide solution in methanesulfonic acid EDCI N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride ESI electrospray ionization Et ethyl Et 2 O diethyl ether EtOAc ethyl acetate EtOH ethyl alcohol FCC flash column chromatography Hantzch ester diethyl 1,4-dihydro-2,6-dimethyl-3,5-pyridinedicarboxylate HATU O-(7-azabenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate Hept heptet HOBt 1-hydroxybenzotriazole HPLC high pressure liquid chromatography Hunig's base N,N-diisopropylethylamine Hz hertz i-PrOH isopropyl alcohol KHMDS potassium bis(trimethylsilyl) amide LCMS liquid chromatography-mass spectrometry LDA lithium diisopropylamide m multiplet M molar (moles/liter) mCPBA meta-chloroperoxybenzoic acid Me methyl Meldrum's acid 2,2-dimethyl-1,3-dioxane-4,6-dione MeOH methanol MHz megahertz min minutes mL milliliters MTBE methyl tertiary butyl ether m/z mass to charge ratio NBS N-bromosuccinimide nm nanometers NaOiPr sodium isopropoxide NMR nuclear magnetic resonance Ph phenyl PPA poly phosphoric acid ppm parts per million Pr propyl q quartet RP-HPLC reverse phase high pressure liquid chromatography s singlet SFC supercritical fluid chromatography t triplet TBAF tetrabutyl ammonium fluoride TEA triethylamine TEMPO (2,2,6,6-tetramethylpiperidin-1-yl)oxidanyl TFA trifluoroacetic acid THF tetrahydrofuran TLC thin layer chromatography UV ultra-violet X-Phos 2-dicyclohexylphosphino-2′,4′,6′-triisopropylbiphenyl

General Schemes:

Compounds of Formula I in the present invention can be synthesized in accordance with the general synthetic methods known to those who are skilled in the art. The following reaction schemes are only meant to represent examples of the invention and are in no way meant to be a limit of the invention.

Scheme 1 describes the preparation of 6-bromo or 6-iodoquinolines of the Formula IV by various methods. As illustrated in path 1, hydroxyquinolin-2(1H)-ones II can be prepared by condensation of readily available 6-bromo or 6-iodoanilines with Meldrum's acid and then subsequently heated in the presence of Eaton's reagent or PPA as described by W. T. Gao, et al. ( Synthetic Communications 2010, 40, 732). Condensation with substituted aldehydes in the presence of a Hantzsch ester, such as diethyl 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate, in solvents like ethanol or pyridine can afford substituted 6-halo-4-hydroxyquinolin-2(1H)-ones III wherein R 6 is C (1-4) alkylQ and Q is defined above. Subsequent heating of quinolines III in the presence of phosphorus oxychloride at temperatures between 80-120° C. with or without a solvent, such as acetonitrile, can provide the 6-bromo or 6-iodoquinolines IV wherein R 5 and R 7 are Cl. Displacement of the 2-Cl of 2,4-dichloroquinoline IV with sodium alkoxides can be accomplished in an alcoholic solvent such as methanol, ethanol or isopropanol or at elevated temperatures in a non-polar solvent such as toluene (Alan Osborne et. al. J Chem. Soc. Perkin Trans. 1 (1993) 181-184 and J. Chem. Research (S), 2002, 4) to provide substituted quinolines IV wherein R 6 is C (1-4) alkylQ and R 5 and R 7 are either Cl or Oalkyl or R 5 and R 7 are both Oalkyl.

Alternatively, as shown in path 2, the 6-haloanilines can be condensed with substituted malonic acids V in phosphorus oxychloride at temperatures between 80-120° C. affording 6-haloquinolines IV wherein R 6 is C(1-4)alkylQ, cycloalkyls (eg. cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl) or saturated heterocycles (eg. tetrahydropyranyl, oxetan-3-yl or tetrahydrofuranyl or 4H-thiopyran-4-yl) and both R 5 and R 7 are Cl. The malonic acids V can be obtained commercially or prepared by addition of ketones to Meldrum's acid and Hantzsch ester, such as diethyl 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate under conditions described by Ramachary, Dhevalapally B. et al ( European Journal of Organic Chemistry 2008 (6) 975-993). Displacement of the chlorines at positions 2 and/or 4 with an Oalkyl can be accomplished as described above to provide 6-haloquinolines of Formula IV wherein R 5 and R 7 are either Cl or Oalkyl or R 5 and R 7 are both Oalkyl.

In path 3, methyl 2-aminobenzoates VI can undergo acylation with acid chlorides VII, obtained from commercial sources or from the corresponding substituted carboxylic acids by treatment with thionyl chloride or oxalyl chloride using known procedures, to form the amide intermediate VIII. The amide can then be further cyclized by treatment with a base such as sodium ethoxide, or by lithium (LiHMDS) or potassium bis(trimethylsilyl)amide (KHMDS) in a solvent such as tetrahydrofuran. Conversion of the resulting hydroxyquinolin-2(1H)-ones III to 2,4-dichloroquinolines can be carried out in refluxing phosphorus oxychloride then subsequently treated with NaOalkyl as described earlier to provide 6-haloquinolines of Formula IV wherein R 6 is OQ, or C (1-4) alkylQ and R 5 and R 7 are either Cl or Oalkyl or R 5 and R 7 are both Oalkyl.

Path 4 illustrates an alternative route to incorporate an oxygen linked substituent at the quinoline C-3 position. The intermediate amides IX can be prepared by the addition of bromoacetyl bromide to the haloaniline VI in the presence of an amine base such as triethylamine or Hunig's base in a chlorinated solvent. The bromine can then be displaced with substituted hydroxyl nucleophiles under basic conditions to provide compounds of Formula VIII wherein R 6 is OQ. Cyclization with a base such as potassium bis(trimethylsilyl)amide followed by sequential steps of chloride addition at the quinoline 2 and 4-position followed by chloride displacement with sodium alkoxide as previously described may afford the 6-haloquinolines of Formula IV wherein R 6 is OQ and R 5 and R 7 are either Cl or Oalkyl or R 5 and R 7 are both Oalkyl.

›Abbreviations · 2 of 5

The chlorines at the 2 and 4-positions of 6-haloquinolines of Formula IV can also be displaced with substituted amines as shown in path 5. Therefore, nucleophilic displacement of the 2-Cl and/or the 4-Cl atom(s) with primary or secondary alkyl amine reagents can be accomplished by heating the 2,4-dichloroquinoline IV with excess amine reagent at temperatures between 80 and 100° C. in an appropriate solvent like DMF to provide the haloquinolines IV wherein R 5 or R 7 are either Cl or NA 3 A 4 or both R 5 or R 7 are NA 3 A 4 .

The synthesis of 6-haloquinolines containing a trialkylsilyl group at the C3-position of the quinoline is described in Scheme 2. Hydroxyquinolin-2(1H)-ones II can be transformed into 2,4-dichloroquinolines X wherein R 5 and R 7 are Cl (path 1) using phosphorous oxychloride.

Treatment with NaOalkyl as previously described to displace the chlorine at the quinoline 2-position followed by deprotonation with a strong base, such as lithium diisopropylamide to afford a 3-lithio quinoline intermediate that can be trapped with a formylating reagent such as dimethylformamide, can provide the intermediate aldehyde that can be subsequently reduced with a reducing reagent, such as sodium borohydride, to furnish the 6-haloquinolines XI. Protection of the primary alcohol functional group with trialkylsilyl chloride reagents furnishes the protected 6-haloquinolines XII. Path 2 describes an alternative sequence starting with 2,4-dichloroquinoline X. Formylation of quinoline X followed by reduction then treatment with trialkylsilyl chloride as previously described can provide the 2,4-dichloroquinoline XII wherein R 5 and R 7 are Cl. Final displacement of the 2-Cl group with NaOalkyl as described above can provide haloquinolines XII wherein R 5 is Cl and R 7 is Oalkyl.

Scheme 3 describes the synthesis of 6-haloquinolines IV containing a methylamino functional group at the C3-position of the quinoline core. As shown in path 1, installing an aldehyde at the 3-position of 2,4-dichloroquinolines X, as previously described, followed by reduction can provide an intermediate 3-hydroxymethylquinoline that can be further chlorinated with thionyl chloride in a solvent such as dichloromethane to provide the corresponding 6-haloquinoline of Formula XIII wherein R 6 is CH 2 Cl. Displacement with mono or disubstituted amine reagents provides the quinolines of Formula IV wherein R 6 is CH 2 NA 3 A 4 . Alternatively, the 3-methylquinolines of Formula IV can be transformed into the bromomethyl quinolines XIV by treatment with N-bromosuccinimide and a radical initiator such as azobisisobutyronitrile (AIBN) or 1,1′-azobis(cyclohexanecarbonitrile) (ABCN) in a solvent such as carbon tetrachloride or benzene (path 2). Displacement of the bromine atom with mono or disubstituted amine reagents in the presence of a base such as N,N-diisopropylethylamine in a solvent such as dichloromethane can also provide 6-haloquinolines IV wherein R 6 is CH 2 NA 3 A 4 .

Scheme 4 outlines routes (paths 1 and 2) to intermediate quinolones XVI containing an ester at the quinoline 3-position. The 4-hydroxyquinolones XVI can be synthesized by condensing haloanilines VI (Z=Br or I) with dialkylmalonates XV in the presence of a base such as a sodium alkoxide in a suitable solvent such as an alcohol (path 1). Alternatively, the 4-hydroxyquinolones XVI can be prepared in two steps from haloaniline VI by first coupling with a 3-chloro-3-oxopropanoate XVII in the presence of a base such as sodium bicarbonate to provide amides XVIII followed by cyclization with a base such as a sodium alkoxide in a solvent such as tetrahydrofuran (path 2).

As shown in path 3, the quinolone esters XVI can be chlorinated with phosphorus oxychloride at temperatures between 80-120° C. with or without a solvent, such as acetonitrile to provide the 6-haloquinolines of Formula XIX wherein R 5 and R 7 are Cl. The 2-Cl substituent can be further displaced with sodium alkoxides as described above to provide the 6-haloquinolines XX wherein R 5 is Cl and R 7 is Oalkyl.

Scheme 5 describes methods used to form 6-haloquinolines of Formula IV wherein R 6 are amides or substituted amines. Saponification of the ester functional group of quinolines XX with metal hydroxides such as lithium or sodium hydroxide in a solvent such as water, tetrahydrofuran, or alcohol(s) (or a mixture thereof) followed by amide bond formation with a coupling reagent such as EDCI, DCC or HATU using condition well known in the art could afford 6-haloquinolines IV wherein R 6 is CONA 3 A 4 (path 1). Base hydrolysis of the esters of Formula XX to form the intermediate carboxylic acid followed by treatment with DPPA and a base such as triethylamine in a solvent like t-butanol at elevated temperatures can provide the intermediate BOC protected amine which can be further treated with an acid such as hydrochloric acid or trifluoroacetic acid to provide the 3-aminoquinolines of Formula XLIII (path 2). The amino group of Formula XLIII can be further elaborated by treatment with a substituted carboxylic acid and a coupling reagent such as EDCI or HATU to provide 6-haloquinolines IV wherein R 6 is NHCOC (1-4) alkylQ. The 3-aminoquinolines XLIII can also be expanded by reductive amination with substituted aldehydes or ketones and sodiumcyanoborohydride or triacetoxyborohydride or by alkylation with substituted bromides or iodides using procedures well known in the art to provide compounds of Formula IV wherein R 6 is NA 3 A 4 or NHC (1-4) alkylQ.

Scheme 6 exemplifies methods used to convert the halogen at C-6 position of the quinoline to an ester. The starting 6-haloquinolines IV can be treated with n-butyl lithium at temperatures ranging from −50 to −78° C., quenched with carbon dioxide then subsequently treated with methyl iodide as described in U.S. Pat. No. 4,710,507 A1, 1987 to provide the methyl ester XXI.

Scheme 7 outlines synthetic routes (path 1 to 5) to aryl ketones of Formula XXVI. In path 1, Weinreb amides XXIII can be prepared from carboxylic acids XXII and N,O-dimethylhydroxylamine hydrochloride in the presence of a base such as triethylamine or Hunig's base and a coupling reagent such as EDCI. The amides XXIII can be further treated with Grignard reagents such as R 2 MgX (X is Br or Cl) XXIV that can be obtained commercially or preformed by treatment of R 2 Z XXV (Z=Br or I) with organometallic reagents such as i-PrMgCl or EtMgCl in THF or dichloromethane to afford the ketones XXVI, wherein R 1 and R 2 are as defined above. As shown in path 2, aldehydes XXVII can also be treated with Grignard reagents to afford the intermediate alcohols XXVIII. Subsequent oxidation with Dess-Martin periodinane or MnO 2 in a suitable solvent such as 1,4-dioxane or tetrahydrofuran at elevated temperatures can provide ketones XXVI. Path 3, which employs palladium catalyzed cross-coupling of arylboronic acids XXIX with acid chlorides XXX using K 3 PO 4 as a base and (Ph 3 P) 2 PdCl 2 as a catalyst in a high boiling non-polar solvent such as toluene, can also be used to generate ketones XXVI. In path 4, aryl ketones XXVI, wherein R 2 is triazolyl, can be prepared by treatment of 1-methyl-1H-1,2,3-triazole, made according to PCT Int. Appl. 2008098104, with n-butyllithium followed by reaction with aldehydes XXVII to yield the secondary alcohols XXVIII, which can undergo oxidation with Dess-Martin periodinane or MnO 2 . Path 5 exemplifies the preparation of symmetrical ketones XXVI, wherein R 1 and R 2 are the same. As illustrated, an aryl or heteroaryl group containing an acidic proton XXXI (Y=R 1 or R 2 ) can be deprotonated in the presence of a strong base such as n-butyllithium in a preferred solvent such as tetrahydrofuran at temperatures between 0 and −78° C. then added in excess to ethyl methoxy(methyl)carbamate to provide aryl ketones XXVI wherein R 1 and R 2 are the same. Aryl or heteroaryl bromide XXXII can also be lithiated through a lithium/halogen exchange with n-butyllithium before adding in excess to ethyl methoxy(methyl)carbamate as previously described to provide symmetrical ketones XXVI.

›Abbreviations · 3 of 5

Scheme 8 illustrates routes for the synthesis of ketoquinolines XXXIII and XXXV. As shown in path 1, treatment of 6-bromo or 6-iodoquinolines IV with n-BuLi followed by addition of aldehydes XXVII, at temperatures between 0 and −78° C., provides secondary alcohol quinolines of Formula I (R 2 is H and R 3 is OH). Oxidation to ketoquinoline XXXIII can be achieved with Dess-Martin periodinane in a solvent such as dichloromethane or with MnO 2 in a solvent such as 1,4-dioxane or tetrahydrofuran at elevated temperatures. Alternatively, 6-bromo or 6-iodoquinolines IV can be treated with n-BuLi at −78° C. then quenched with DMF to afford quinoline carboxaldehydes XXXIV (path 2). Ketoquinolines XXXV, wherein Y is R 1 or R 2 , can then be obtained in a two-step process by addition of the aldehydes XXXIV to a reaction mixture of aryl halides XXXII (Y=R 1 or R 2 and Z=Br or I) and i-PrMgCl.LiCl (or n-BuLi) followed by oxidation with MnO 2 (path 2). Reduction of the ketoquinolines XXXV with sodium borohydride can offer an additional method to secondary alcohols of Formula I wherein R 2 is H and R 3 is OH.

Scheme 9 exemplifies synthetic methods that could be used to prepare compounds of Formula I (paths 1-4). As illustrated in path 1, 6-bromo or 6-iodoquinolines IV in an appropriate solvent such as THF can be either premixed with the ketones XXVI at −78° C. followed by addition of n-BuLi or can be pretreated with n-BuLi at −78° C. prior to the addition of the ketones XXVI to afford the tertiary alcohols of Formula I, wherein R 3 is OH.

Path 2 illustrates the formation of tertiary alcohols of Formula I by treatment of the ketoquinolines XXXV (Y is R 1 or R 2 ) with Grignard reagents XXXVI that are either commercially available or can be prepared by a halogen-metal exchange of aryl halides XXXII with ethyl or isopropyl magnesium chloride as previously described. Similarly, as shown in path 3, an organometallic reagent, such as n-BuLi can be added to an aryl halide XXXII at temperatures between −78° C. and ambient temperature in a preferred solvent such as tetrahydrofuran followed by the addition of the ketoquinolines XXXV to afford the tertiary alcohols of Formula I wherein R 3 is OH and R 1 and R 2 are as defined above. Path 4 describes a method that can be used to incorporate an R 2 alkyl group by treating ketoquinolines XXXIII with an alkyl lithium at a temperature between −78 and −40° C. once solubilized in an appropriate solvent such as tetrahydropyran to provide quinolines of Formula I wherein R 2 is alkyl and R 3 is OH.

The ketoquinolines XXXIII can also be treated with a protected alkynyl lithium such as TMS-lithiumacetylide at temperatures between 0° C. and ambient temperature in a solvent such as THF followed by deprotection with a base such as KOH in a polar alcohol solvent such as methanol or ethanol to provide compounds of Formula I where in R 2 is acetylene and R 3 is OH (path 5).

Scheme 10 depicts an additional sequence to form compounds of the general Formula I wherein R 6 is a dialkyamine (CH 2 NA 3 A 4 ). The tertiary alcohols of Formula XXXVII can be prepared from the 6-haloquinoline XII as described above then deprotected with a fluoride reagent such as tetra-butylammonium fluoride to provide a primary alcohol at the C3-position. This primary alcohol intermediate can be transformed into quinoline benzyl chlorides XXXVIII using thionyl chloride as previously described. Displacement of the chlorine atom by amines such as substituted piperidine, aziridine, morpholine, piperazine and alike, can provide compounds of the general Formula I wherein R 6 is CH 2 NA 3 A 4 .

Scheme 11 illustrates methods used to synthesize compounds of Formula I wherein either the chlorine at R 7 or R 5 or at both R 5 and R 7 positions are replaced with nitrogen, oxygen, sulfur or alkyl groups. In path 1 and 4, nucleophilic displacement of 2,4-dichloroquinolines I (R 5 and R 7 are Cl) with NaO(alkyl) or NaS(alkyl), such as NaOMe, NaSMe, NaOEt, or NaOiPr, in an appropriate solvent, such as MeOH, EtOH, i-PrOH or DMF at elevated temperatures or with substituted hydroxy reagents such as 2-methoxyethanol in the presence of a base like sodium hydride in a non-polar solvent such as toluene provides compounds of Formula I wherein R 5 is Cl and R 7 is O(alkyl), O(CH 2 ) 2 OCH 3 or S(alkyl) and compounds of Formula I wherein R 5 and R 7 are O(alkyl) or S(alkyl). Likewise, nucleophilic displacement of 2,4-dichloroquinolines I (R 5 and R 7 are Cl) with primary or secondary alkyl amines, heterocyclic amines, or N,O-dimethylhydroxylamine in polar solvents such as MeOH, EtOH, or Et 2 NCHO, or DMF provides quinolines of Formula I (path 2) wherein R 5 and R 7 are either Cl or NA 1 A 2 , wherein A 1 and A 2 are as defined above. Introduction of cyclic amides can be accomplished using Buchwald palladium catalyzed coupling conditions to provide compounds of Formula I, wherein R 7 are rings such as azetidin-2-ones or pyrrolidin-2-ones. Replacement of chlorine at positions 2 and 4 of quinolines I (R 5 and R 7 are Cl) with alkyl groups can be carried out using Zn(alkyl) 2 in the presence of K 2 CO 3 and a palladium catalyst, such as PdCl 2 (dppf), to afford 2-alkyl and 2,4-dialkylquinolines of Formula I (path 3). Displacement of chlorine at the 2-position of 2,4-dichloroquinolines I with methylsulfone can be accomplished with methanesulfinic acid in a solvent such as DMF under elevated temperature between 90 and 110° C. (path 5).

Compounds of Formula I, wherein R 1 and R 2 are the same, can also be prepared as described in Scheme 12. The intermediate quinoline methyl ester XXI can be treated with excess YLi, or YMgBr, in the presence or absence or lanthanum chloride, to afford the symmetrical compounds of Formula I.

Scheme 13 describes a path to the introduction of trifluoromethyl groups at the quinoline 2 and 4-positions. The starting aniline XXXIX, prepared as described in Tetrahedron Letters (1986) 27, 1423-1424 and JMC (2009) 52, 7289-7300, can be converted to the quinoline esters of Formula XX (R 5 and R 7 are CF 3 ) by heating with alkyl 4,4,4-trifluoro-3-oxobutanoate and a base like piperidine in an alcoholic solvent like ethanol as described in WO2010/112826. The quinoline esters of Formula XX could then be further elaborated as described above to provide quinolines of Formula I wherein R 6 is CO 2 alkyl and R 5 and R 7 are CF 3 .

›Abbreviations · 4 of 5

Scheme 14 outlines synthetic methods used to elaborate the quinoline 3-position of compounds of Formula I. Palladium-catalyzed hydrogenation of compounds of Formula I wherein R 6 is benzyloxy can provide intermediate quinolin-3-ols XL. As shown in path 1, the quinoline-3-ol XL can be substituted by a displacement reaction (Mitsunobu reaction) in the presence of a dialkylazodicarboxylate, such as diisopropropylazodicarboxylate, and a triaryl phosphine, such as triphenylphosphine to provide compounds of Formula I wherein R 6 is OQ. Alternatively, the quinolin-3-ol XL can be converted into the corresponding triflate XLI with trifluoromethanesulfonic acid in the presence of a base, such as pyridine, in a solvent such as dichloromethane (path 2). Palladium-catalyzed cross coupling of the triflate XLI with organoboron reagents of the formula R 6 B(OR) 2 in the presence of a base, such as potassium carbonate, in a solvent mixture such as 1,4-dioxane/water can provide compounds of Formula I wherein R 6 is a substituted or unsubstituted carbo or heterocyclic ring containing a double bond (eg. 1-methyl-1,2,3,6-tetrahydropyridin-4-yl, 2,2,6,6-tetramethyl-3,6-dihydro-2H-pyran-4-yl, 1-((trifluoromethyl)sulfonyl)-1,2,3,6-tetrahydropyridin-4-yl, 4,4-difluorocyclohex-1-en-1-yl, cyclopenten-1yl, etc. and alike). The double bond can be reduced by palladium catalyzed hydrogenation to provide compounds of Formula I wherein R 6 is a substituted or unsubstituted saturated carbo or heterocyclic ring.

Scheme 15 details the synthesis of compounds of Formula I, wherein R 5 and R 7 are further modified. In path 1, cyanation of the 2,4-dichloroquinolines I (R 5 and R 7 are Cl) with Zn(CN) 2 in the presence of Zn, a palladium catalyst, such as Pd 2 (dba) 3 , and a ligand, such as dppf or X-Phos, at high temperatures can provide 2-CN and 2,4-diCN quinolines of Formula I.

The 2,4-dichloroquinolines I can also undergo a Suzuki palladium catalyzed cross-coupling reaction with alkyl or aryl boronic acids or esters with a palladium catalyst, such as PdCl 2 (dppf), yielding compounds of Formula I wherein R 7 is alkyl, aryl or heteroaryl (path 2).

Scheme 16 describes additional methods that can be used to prepare compounds of Formula I wherein R 6 is 4-methyltetrahydro-2H-thiopyran 1,1-dioxide by treating quinolines of Formula XLII with 3-chlorobenzoyl peroxide in the presence of phosphorus tribromide in a solvent mixture such as dichloromethane and dimethylformamide.

As illustrated in Scheme 17, compounds of Formula I wherein only R 5 is a chlorine can be further substituted by treatment with alkylboronic acids or esters under Suzuki reaction conditions (path 1), with sodium alkoxides (path 2), or with zinc cyanide (path 3) using conditions previously described to provide compounds of Formula I wherein R 5 is alkyl, O(alkyl) or CN and R 7 is as defined above.

As shown in Scheme 18, tertiary alcohols of Formula I can be treated with base, such as NaH, and alkylated with MeI in DMF to provide compounds of Formula I wherein R 3 is OMe.

Synthetic routes to compounds of Formula I, wherein R 3 is NH 2 , are illustrated in Scheme 19. Ketimines XLIII may be prepared by Ti(OEt) 4 mediated condensation of ketones XXVI with 2-methylpropane-2-sulfinamide in refluxing THF. Addition of n-BuLi to the reaction mixture of ketimines XLIII and 6-bromo or 6-iodoquinolines IV at −78° C. followed by cleavage of the tert-butanesulfinyl group with HCl in MeOH liberates tertiary amines of Formula I.

Alternatively, compounds of Formula I, wherein R 3 is OH can be treated with sodium hydride followed by addition of acetic anhydride or acetyl chloride and stirred at room temperature over a 24 to 72 hour period to provide the intermediate acetate wherein R 3 is OAc. The acetate can then be combined with a solution of ammonia in methanol and heated at temperatures between 60 and 85° C. to provide compounds of Formula I, wherein R 3 is NH 2 .

As shown in Scheme 20, the quinolines of Formula I wherein R 7 is CN can be hydrolyzed as described in US20080188521 by treatment with sodium carbonate and hydrogen peroxide to provide compounds of Formula I wherein R 7 is CONH 2 (path 1) or can be treated with a strong acid like HCl to convert CN to a carboxylic acid XLIV (path 2). Once formed, the acid can be further coupled to substituted amines using appropriate coupling reagents such as EDCI or HATU in the presence of a base like triethylamine or Hunig's base to provide compounds of Formula I wherein R 7 is CONA 1 A 2 .

Synthesis of compounds of Formula I, wherein R 7 is an aminoalkylaminomethylene or an aminoalkoxymethylene can be prepared from 2-methylquinolines as shown in Scheme 21. Bromination of 2-methylquinolines of Formula I can be accomplished with N-bromosuccinimide in acetic acid at elevated temperatures as described in WO2010151740, to provide the methylbromide intermediate XLV. Nucleophilic displacement of the bromide under basic conditions using procedures known in the art could afford compounds of Formula I wherein R 7 is —CH 2 NHC (2-3) alkylNA 1 A 2 or —CH 2 N(CH 3 )C (2-3) alkylNA 1 A 2 (path 1) or CH 2 OC (2-3) alkylNA 1 A 2 (path 2) and A 1 and A 2 are as defined above.

Compounds of Formula I wherein R 1 , R 2 or R 6 are or contain a pyridyl can be treated with m-chloroperbenzoic acid in a chlorinated solvent at ambient temperature to 40° C. to form the pyridyl-N-oxides of Formula I.

Precursors to compounds of formula IV, wherein R 8 is H or CH 3 , can be prepared from methyl-2-nitrobenzoates XLVI by first hydrogenation of the nitro group in the presence of Raney nickel in a solvent such as methanol followed by bromination with N-bromosuccinimide in dichloromethane to provide methyl-2-aminobenzoates VI (Scheme 22). Methyl-2-aminobenzoates VI can be transformed into compounds of formula IV as detailed above.

Compounds of formula I, wherein R 6 is —OCH 2 CF 3 can be prepared as shown in Scheme 23. Treatment of compounds of formula XL with 2,2,2-trifluororethyl trifliuoromethanesulfonate in the presence of a base such as cesium carbonate in a solvent such as tetrahydrofuran affords compounds of formula I (R 6 is —OCH 2 CF 3 ).

›Abbreviations · 5 of 5

Compounds of formula XXI, wherein R 6 is —CH 2 Br or —CH 2 OTBS can be prepared as shown in Scheme 24. Starting with condensation of 4-amino benzoates with malonic acids V (R 6 =CH 3 ) in the presence of phosphorus oxychloride followed by treatment with NaOalkyl as described above affords compounds of formula XXI (R 6 =CH 3 ). Benzylic halogenation with a bromination reagent such as N-bromosuccinimide in the presence of a radical initiator such as azobisisobutyronitrile in a solvent such as carbon tetrachloride provides compounds of formula XXI (R 6 =CH 2 Br). Conversion of the benzylic bromide into the benzylic alcohol can be achieved with a reagent such as silver sulfate in a solvent mixture such as water/dioxane and, following protection of the alcohol functional group with tert-butylchlorodimethylsilane (TBSCl) in the presence of imidazole in a solvent such as dimethylformamide, furnishes the compound of formula XXI (R 6 =CH 2 OTBS).

Compounds of formula I, wherein R 1 and R 2 are the same and R 6 is —CH 2 NA 3 A 4 , can also be prepared as described in Scheme 25. Treatment of compounds of the formula XXI [R 6 =CH 2 OSi(alkyl) 3 ] with excess YLi or YMgBr affords compounds of formula XXXVII. Treatment with trifluoroacetic acid in a solvent such as dichloromethane followed by chlorination with a reagent such as thionyl chloride in a solvent such as tetrahydrofuran affords compounds of formula XXXVIII. Displacement of the chlorine atom by amines in the presence of a base such as triethylamine with or without the addition of potassium iodide can provide compounds of the general formula I, wherein R 6 is —CH 2 NA 3 A 4 .

Compounds of formula I, wherein R 1 and R 2 are the same and R 6 is —CH 2 NA 3 A 4 , can also be prepared as described in Scheme 26. Displacement of the bromine atom in XXI (R 6 =CH 2 Br) with diallylamine affords XLVII. Addition of excess YLi or YMgBr affords compounds of formula XLVIII. Removal of the allyl groups with tetrakis(triphenylphosphine)palladium (0) and 1,3-dimethylpyrimidine-2,4,6(1H,3H,5H)-trione followed by amide bond formation using standard coupling conditions provided compounds of the formula I (R 6 =CH 2 NA 3 A 4 )

Compounds of the general formula XXXIII can be formed by coupling of the organolithium or organomagnesium intermediate derived from compounds of the formula IV with amides of the general structure XXIII (Scheme 27).

Compounds of the general formula I, wherein R 2 is N-acyl- or sulfonyl-azetidinyl or N-acyl- or sulfonyl-piperidinyl can be prepared as detailed in Scheme 28. N-Boc protected compounds of the general formula I (R 2 =N-Boc-azetidin-3-yl or N-Boc-piperidin-4-yl) are treated with an acid such as trifluoroacetic acid in a solvent such as dichloromethane to remove the Boc protecting group. Acylation or sulfonylation with reagents such as acetic anhydride/acid chlorides or sulfonyl chlorides, respectively, in the presence of a base such as triethylamine, affords compounds of the formula I (R 2 is N-acyl- or sulfonyl-azetidinyl or N-acyl- or sulfonyl-piperidinyl).

Compounds of the general formula I, wherein R 7 is alkyl or cycloalkyl can be prepared as detailed in Scheme 29. Coupling of 6-bromo-1H-benzo[d][1,3]oxazine-2,3-dione (5-bromoisatoic anhydride) with 3-alkyl/cycloalkyl-3-oxopropanoates (XLIX, pre-treated with a base such as sodium hydride) in a solvent such as dimethylacetamide followed by chlorination with phosphorus oxychloride as described previously affords compounds of the general formula XIX (R 5 =Cl, Z=Br). Reduction of the ester functional group with a reagent such as DIBAL-H provides compounds of the general formula XIX. Transformation of the alcohol functional group into the corresponding alkyl chloride with a reagent such as methane sulfonylchloride in the presence of a base such as diisopropylethyl amine furnishes compounds of the general formula XIII. Compounds of the general formula I can be synthesized by displacement of the alkyl chloride by amines and formation of the tertiary alcohol as described previously.

›EXAMPLES · 1 of 31

Compounds of the present invention can be prepared by methods known to those who are skilled in the art. The following examples are only meant to represent examples of the invention and are in no way meant to be a limit of the invention.

Intermediate 1: Step a

6-Bromo-2,4-dichloroquinoline

Into a 250-mL round-bottom flask was placed a solution of 4-bromoaniline (10.0 g, 58.13 mmol, 100%) and propanedioic acid (6.4 g, 61.50 mmol) in POCl 3 (30 mL). The resulting solution was stirred for 12 hours at 100° C. The reaction was then quenched by the addition of 200 mL of water/ice. The resulting solution was extracted with 3×100 mL of ethyl acetate. The combined organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. The residue was purified by chromatography over a silica gel column with ethyl acetate/petroleum ether (1:50-1:10) to afford the title compound as a white solid.

Intermediate 1: Step b

6-Bromo-2,4-dichloroquinoline-3-carbaldehyde

Into a 100-mL round-bottom flask was placed a solution of bis(propan-2-yl)amine (1.44 g, 14.23 mmol, 100%) in 20 mL THF, and then n-BuLi (5.24 mL, 13.1 mmol, 2.5 M in hexanes) at −78° C. After 30 minutes, 6-bromo-2,4-dichloroquinoline (3.3 g, 11.92 mmol, Intermediate 1: step a) was added. The resulting solution was stirred for 1 hour at −78° C. A solution of N,N-dimethylformamide (1.04 g, 14.23 mmol) in tetrahydrofuran (30 mL) was then added. The resulting solution was stirred for an additional 5 hours at −78° C. The reaction was then quenched by the addition of 10 mL of water. The resulting solution was extracted with 3×50 mL of ethyl acetate. The combined organic layers were dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. The residue was purified by chromatography over a silica gel column with dichloromethane/petroleum ether (100:1) to afford the title compound as a yellow solid.

Intermediate 1: Step c

(6-Bromo-2,4-dichloroquinolin-3-yl)methanol

Into a 100-mL round-bottom flask was placed a solution of 6-bromo-2,4-dichloroquinoline-3-carbaldehyde (1.5 g, 4.92 mmol, Intermediate 1: step b) in tetrahydrofuran (20 mL). NaBH 3 CN (930 mg, 14.80 mmol) was then added and the resulting solution was stirred for 12 hours at 25° C. The resulting mixture was concentrated under vacuum. The residue was purified by chromatography over a silica gel column and eluted with ethyl acetate/petroleum ether (1:4) to afford the title compound as a white solid.

Intermediate 1: Step d

6-Bromo-2,4-dichloro-3-(chloromethyl)quinoline

Into a 250-mL round-bottom flask, was placed a solution of (6-bromo-2,4-dichloroquinolin-3-yl) methanol (650 mg, 2.12 mmol, Intermediate 1: step c) and thionyl chloride (2.5 g, 21.2 mmol) in dichloromethane (100 mL). The resulting solution was stirred for 12 hours at 25° C. The resulting mixture was concentrated under vacuum. The residue was purified by chromatography over a silica gel column and eluted with ethyl acetate/petroleum ether (1:10) to afford the title compound as a white solid.

Intermediate 1: Step e

4-((6-Bromo-2,4-dichloroquinolin-3-yl)methyl)morpholine

Into a 100-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed a solution of morpholine (88.4 mg, 1.01 mmol), sodium hydride (44.3 mg, 1.11 mmol, 60%) and 6-bromo-2,4-dichloro-3-(chloromethyl)quinoline (300 mg, 0.92 mmol, Intermediate 1: step d) in N,N-dimethylformamide (10 mL). The resulting mixture was stirred for 2 hours at room temperature. The reaction was then quenched by the addition of 30 mL of water. The resulting solution was extracted with 3×20 mL of ethyl acetate. The combined organic layer was dried (Na 2 SO 4 ), filtered and concentrated under vacuum. The residue was purified by chromatography over a silica gel column with ethyl acetate/petroleum ether (1:4) to afford the title compound as a white solid.

Intermediate 2: Step a

1-(5-Bromo-2-fluorophenyl)-2,2,2-trifluoroethanone

A solution of diisopropylamine (22.1 mL, 157 mmol) in 140 mL THF was stirred under argon at −68° C. while n-BuLi (57.9 mL, 2.59 M in hexane, 150 mmol) was added in a fine stream in 2 portions over 6 minutes. The resulting pale yellow homogeneous solution was removed from the acetone/dry ice bath and stirred at ambient conditions for 9 minutes, and was then cooled back down to −68° C. and a solution of 1-bromo-4-fluorobenzene (15.6 mL, 143 mmol) in THF (30 mL) was added rapidly dropwise over 5 minutes. The reaction was then stirred in the cold bath for another 6 minutes, and the pale yellow reaction was then treated rapidly dropwise with a solution of ethyl trifluoroacetate (18.7 mL, 157 mmol) in THF (30 mL) over ˜8 minutes (internal temp rose to −47° C.). The pale yellow reaction was then stirred overnight as the acetone/dry ice bath expired (15 hours). The resulting yellow homogeneous solution was washed with 5 M aqueous NH 4 Cl (2×50 mL), and the organic layer was dried (Na 2 SO 4 ), filtered, and concentrated to provide the crude title compound as a clear dark yellow oil.

Intermediate 2: Step b

1-(2-Amino-5-bromophenyl)-2,2,2-trifluoroethanone

A solution of 1-(5-bromo-2-fluorophenyl)-2,2,2-trifluoroethanone (6.67 g, 24.6 mmol, Intermediate 2: step a) in DMSO (6.2 mL) was treated with NaN 3 (1.76 g, 27.0 mmol) and stirred under air (lightly capped) at 95° C. for 1 hour. The brownish-red opaque reaction was then cooled to room temperature on an ice bath, diluted with EtOAc (49 mL), treated with SnCl 2 .dihydrate (6.66 g, 29.5 mmol) in several portions over ˜30 seconds followed by water (1.33 mL, 73.8 mmol), and the mixture was stirred at room temperature for 30 minutes. The reddish solution with heavy off-white particulates was then treated with anhydrous Na 2 SO 4 (˜6 g; ˜40 mmol; ˜400 mmol water capacity) and stirred vigorously for a few minutes. The mixture was then filtered over a bed of Celite®, and the cloudy orange filtrate was dry load flash chromatographed (˜60 g silica gel) with a heptane to 50% DCM/heptane gradient to provide the title compound as an orange oil that crystallized upon standing.

›EXAMPLES · 2 of 31

Intermediate 2: Step c

Ethyl 6-bromo-2,4-bis(trifluoromethyl)quinoline-3-carboxylate

A mixture of 1-(2-amino-5-bromophenyl)-2,2,2-trifluoroethanone (0.416 g, 1.55 mmol, Intermediate 2: step b), ethyl 4,4,4-trifluoro-3-oxobutanoate (0.286 g, 1.55 mmol), piperidine (0.153 mL, 1.55 mmol), and EtOH (0.5 mL) was heated in a microwave reactor at 130° C. for 30 minutes (Biotage® Initiator). The homogeneous amber solution was concentrated and the residue flash chromatographed (2%-50% DCM in heptane) to provide the title compound as a clear yellow oil.

Intermediate 3: Step a

tert-Butyl 4-((4-fluorophenyl)(hydroxy)methyl)piperidine-1-carboxylate

A solution of tert-butyl 4-formylpiperidine-1-carboxylate (10.4 g, 48.7 mmol) in THF (70 mL) was stirred at ˜−70° C. under argon while 4-fluorophenylmagnesium bromide (1.10 M in THF, 45.6 mL, 50.1 mmol) was added dropwise over 9 minutes. The reaction was then immediately removed from the cold bath and allowed to warm to room temperature with stirring. The yellow homogeneous reaction was allowed to sit at room temperature for 2 days, and was then quenched with 5 M aqueous NH 4 Cl (20 mL) and partitioned with MTBE (25 mL). The aqueous layer was extracted with MTBE (25 mL), and the combined organic layers were dried (Na 2 SO 4 ), filtered, and concentrated. The residue was flash chromatographed (0-100% EtOAc in heptane) to provide the title compound as a clear colorless thick oil.

Intermediate 3: Step b

tert-Butyl 4-((4-fluoro-3-(2,2,2-trifluoroacetyl)phenyl)(hydroxy)methyl)piperidine-1-carboxylate

A translucent solution of tert-butyl 4-((4-fluorophenyl)(hydroxy)methyl)piperidine-1-carboxylate (3.24 g, 10.5 mmol, Intermediate 3: step a), N,N,N′,N′,N″-pentamethyldiethylenetriamine (4.59 mL, 22.0 mmol), and KOtBu (1.02 M in THF, 21.6 mL, 22.0 mmol) was stirred at −70° C. (internal temperature) while sec-BuLi (1.42 M in cyclohexane, 15.5 mL, 22.0 mmol) was added dropwise over 9 minutes. After stirring for an additional 20 minutes, 2,2,2-trifluoro-N-methoxy-N-methylacetamide (2.66 mL, 22.0 mmol) was added dropwise over 5 minutes and stirred for an additional 10 minutes. The reaction was then quenched with 5 M aqueous NH 4 Cl (20 mL) and extracted with MTBE (1×20 mL, 1×30 mL). The combined organic layers were washed with 1 M aqueous NaH 2 PO 4 (2×25 mL), dried (Na 2 SO 4 ), filtered, and concentrated to provide the title compound as a clear yellow oil.

Intermediate 3: Step c

tert-Butyl 4-(4-fluoro-3-(2,2,2-trifluoroacetyl)benzoyl)piperidine-1-carboxylate

A solution of tert-butyl 4-((4-fluoro-3-(2,2,2-trifluoroacetyl)phenyl)(hydroxy)methyl)piperidine-1-carboxylate (4.45 g, 11.0 mmol, Intermediate 3: step b) and TEMPO (51.5 mg, 0.329 mmol) in DCM (22 mL) was treated with a solution of KBr (131 mg, 1.10 mmol) in 1 M aqueous NaHCO 3 (3.84 mL, 3.84 mmol) while stirring in an ice bath. NaOCl (0.89 M in water, 6.15 w/w % Clorox® bleach, 13.6 mL, 12.1 mmol) was then added dropwise over 4 minutes (internal temperature stayed below 14° C.). After 30 minutes stirring in the ice bath, the aqueous layer was extracted with CHCl 3 (2×20 mL). The combined organic layers were washed with 5 M aqueous NaCl (40 mL), dried (Na 2 SO 4 ), and concentrated to provide the crude title compound as a clear dark yellow thick oil that was used without further purification.

Intermediate 3: Step d

tert-Butyl 4-(4-amino-3-(2,2,2-trifluoroacetyl)benzoyl)piperidine-1-carboxylate

A solution of tert-butyl 4-(4-fluoro-3-(2,2,2-trifluoroacetyl)benzoyl)piperidine-1-carboxylate (3.67 g, 9.10 mmol, Intermediate 3: step c) in DMSO (2.25 mL) was bubbled with ammonia for 1 minute in a 200 mL capacity round bottomed pressure flask, and was then sealed and stirred at 100° C. for 2 hours. The reaction was then cooled to room temperature and partitioned with MTBE (20 mL) and 1 M aqueous NaHCO 3 (20 mL), and the aqueous layer was extracted with MTBE (20 mL). The combined organic layers were dried (Na 2 SO 4 ), filtered, and concentrated to dryness. The residue was dry load flash chromatographed (0-50% acetone in heptane) to provide the title compound as a thick orange oil.

Intermediate 3: Step e

(Z)-4,4,4-Trifluoro-3-hydroxy-1-(piperidin-1-yl)but-2-en-1-one

A solution of ethyl 4,4,4-trifluoro-3-oxobutanoate (20 g, 108 mmol) in m-xylene (20 mL) was treated with piperidine (9.66 mL, 97.8 mmol) in one portion at room temperature. The reaction immediately became hot, and this was refluxed under air (heating mantle 170° C.) for 30 minutes. The reaction was cooled to room temperature and vacuum distilled through a short path Vigreaux microdistillation apparatus and vacuum re-distilled through a ˜5″ Vigreaux column to provide the title compound as a clear very pale yellow oil.

Intermediate 3: Step f

tert-Butyl 4-(3-(piperidine-1-carbonyl)-2,4-bis(trifluoromethyl)quinoline-6-carbonyl)piperidine-1-carboxylate

A solution of tert-butyl 4-(4-amino-3-(2,2,2-trifluoroacetyl)benzoyl)piperidine-1-carboxylate (0.964 g, 2.36 mmol, Intermediate 3: step d) and (Z)-4,4,4-trifluoro-3-hydroxy-1-(piperidin-1-yl)but-2-en-1-one (0.665 g, 2.98 mmol, Intermediate 3: step e) in DMF (2.4 mL) was treated with tributylamine (0.618 mL, 2.60 mmol) and stirred at 130° C. for 3 hours. The reaction was cooled to room temperature and partitioned with diethyl ether (8 mL) and 1 M aqueous NaH 2 PO 4 (8 mL). The organic layer was washed with 1 M aqueous NaH 2 PO 4 (8 mL), dried (Na 2 SO 4 ), filtered, and concentrated. The residue was flash chromatographed (0-50% acetone in heptane over 20 column volumes) to provide the title compound as a clear light amber thick oil.

Intermediate 4: Step a

4-Hydroxy-6-iodoquinolin-2(1H)-one

A mixture of 4-iodoaniline (13.1 g, 59.7 mmol) and Meldrum's acid (8.61 g, 59.7 mmol) was stirred at 85° C. for 1 hour, and a stream of nitrogen gas was then blown onto the reaction for 15 minutes to remove acetone. The resulting dark semi-solid was then removed from the heating block and Eaton's Reagent (7.4 w/w % P205, 88.1 mL, 68.7 mmol) was added in one portion. The reaction was stirred for 3 hours at 75° C., and the reaction was then stirred in an ice bath while water (180 mL) was slowly poured in as a constant stream. The reaction was stirred in the ice bath for ˜15 minutes and then filtered. The brown filter cake was washed with water (2×25 mL) and air dried at 110° C. to provide the title compound as a light brown powder.

›EXAMPLES · 3 of 31

Intermediate 4: Step b

2,4-Dichloro-6-iodo-3-((tetrahydro-2H-pyran-4-yl)methyl)quinoline

A mixture of 4-hydroxy-6-iodoquinolin-2(1H)-one (7.20 g, 25.1 mmol, Intermediate 4: step a), tetrahydro-2H-pyran-4-carbaldehyde (3.20 g, 28.0 mmol), and diethyl 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate (7.06 g, 27.9 mmol) in pyridine (50 mL) was stirred under argon at 130° C. for 30 minutes. The reaction was concentrated, and the residue was treated with POCl 3 (23.5 mL, 252 mmol) and stirred at 90° C. for 1 hour. The reaction was cooled to room temperature, diluted with DCM (75 mL), and stirred in an ice bath while ice water (50 mL) was poured in one portion. The dark solution was stirred in the ice bath for a few minutes and then at room temperature for 30 minutes. The aqueous layer was partitioned between water (100 mL) and DCM (40 mL), and the organic layers were combined and washed with water (2×100 mL), dried (Na 2 SO 4 ), filtered, and concentrated. The residue was purified by FCC (DCM isocratic elution) twice to afford the title compound as a thick dark yellow oil.

Intermediate 4: Step c

4-Chloro-6-iodo-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinoline

A mixture of 2,4-dichloro-6-iodo-3-((tetrahydro-2H-pyran-4-yl)methyl)quinoline (3.59 g, 8.51 mmol, Intermediate 4: step b), sodium methoxide (4.61 g, 85.3 mmol), and toluene (85 mL) was stirred at 100° C. under argon for 22 hours. The reaction was cooled to room temperature, filtered, and the filter cake was washed with DCM (50 mL). The combined filtrates were concentrated to provide the title compound as a light yellow solid.

Intermediate 5

tert-Butyl 4-(4-chloro-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinoline-6-carbonyl)piperidine-1-carboxylate

A solution of 4-chloro-6-iodo-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinoline (692 mg, 1.66 mmol, Intermediate 4: step c) in THF (5.6 mL) was stirred at ˜−70° C. under argon while n-BuLi (1.63 M in hexane, 0.97 mL, 1.58 mmol) was added dropwise over 2.5 minutes. After stirring an additional 1 minute, a solution of tert-butyl 4-(methoxy(methyl)carbamoyl)piperidine-1-carboxylate (538 mg, 1.98 mmol) in THF (1 mL) was added dropwise over 45 seconds. The reaction was stirred in the cold bath for 1 hour, removed from the cold bath and stirred at ambient conditions for 20 minutes, and was then quenched with 5 M aqueous NH 4 Cl (0.5 mL). The organic layer was dried (Na 2 SO 4 ), filtered, and concentrated, and the residue was flash chromatographed (0-50% EtOAc in heptane over 18 column volumes) to provide the title compound as an off-white crystalline solid.

Intermediate 6: Step a

Methyl 5-iodo-2-(2-(tetrahydro-2H-pyran-4-yl)acetamido)benzoate

A solution of methyl 2-amino-5-iodobenzoate (7.02 g, 25.3 mmol) and 2-(tetrahydro-2H-pyran-4-yl)acetic acid (3.76 g, 26.1 mmol) in pyridine (25 mL) was stirred at ˜−40° C. under argon while POCl 3 (2.58 g, 27.8 mmol) was added dropwise over 3 minutes. The cold bath was immediately removed and the reaction was allowed to stir for 1 hour. The reaction was then diluted with water (75 mL) and extracted with DCM (75 mL). The combined orange organic layers were washed with 6 M aqueous HCl (50 mL), 1 M aqueous HCl (50 mL), and 2 M aqueous K 3 PO 4 (50 mL), dried (Na 2 SO 4 ), filtered, and concentrated to provide the title compound as an orange solid.

Intermediate 6: Step b

4-Hydroxy-6-iodo-3-(tetrahydro-2H-pyran-4-yl)quinolin-2(1H)-one

A solution of methyl 5-iodo-2-(2-(tetrahydro-2H-pyran-4-yl)acetamido)benzoate (7.21 g, 17.9 mmol, Intermediate 6: step a) in THF (179 mL) was stirred on a dry ice/acetone bath under argon while sodium bis(trimethylsilyl)amide (1.01 M in THF, 37.2 mL, 37.6 mmol) was added dropwise over 6 minutes. The reaction was stirred for 20 minutes, and then removed from the cold bath and stirred at ambient conditions for 100 minutes. LCMS showed the reaction had stalled with only a few % conversion, so the reaction was chilled to ˜−70° C. again and treated with additional sodium bis(trimethylsilyl)amide (1.01 M in THF, 16.8 mL, 17.0 mmol) dropwise over 4 minutes, and stirred for 15 minutes before removing the cold bath and allowing the reaction to stir under ambient conditions overnight. LCMS after 15 hours showed a ˜2:1:1 ratio of methyl 5-iodo-2-(2-(tetrahydro-2H-pyran-4-yl)acetamido)benzoate/5-iodo-2-(2-(tetrahydro-2H-pyran-4-yl)acetamido)benzoic acid/title compound. Therefore, the reaction was chilled in a dry ice/acetone bath again while potassium bis(trimethylsilyl)amide (0.52 M in toluene, 15.5 mL, 8.05 mmol) was added dropwise over 3 minutes, the cold bath was immediately removed, and the reaction was allowed to stir under ambient conditions for 3 hours. LCMS showed ˜no further conversion to title compound, so the reaction was quenched with 6 M aqueous HCl (21 mL, 126 mmol) and the organic layer was dried (Na 2 SO 4 ), filtered, and concentrated to provide the impure title compound that was used without further purification.

Intermediate 6: Step c

2,4-Dichloro-6-iodo-3-(tetrahydro-2H-pyran-4-yl)quinoline

A mixture of very impure 4-hydroxy-6-iodo-3-(tetrahydro-2H-pyran-4-yl)quinolin-2(1H)-one (7.74 g crude, “20.9 mmol”, Intermediate 6: step b) and POCl 3 (19.4 mL, 208 mmol) was stirred at 90° C. for 2.5 hours. The reaction was then cooled to room temperature, dissolved in DCM (100 mL), stirred in an ice bath for ˜10 minutes, and then treated with ice water (100 mL) in one portion. This was stirred in the ice bath for ˜45 minutes, and the resulting slurry was filtered over a bed of Celite®. The clear dark organic layer filtrate was dried (Na 2 SO 4 ), filtered, and concentrated, and the residue was dry load flash chromatographed (0-30% EtOAc in heptanes over 13 column volumes) to afford the title compound as a yellow solid.

Intermediate 6: Step d

4-Chloro-6-iodo-2-methoxy-3-(tetrahydro-2H-pyran-4-yl)quinoline

A mixture of 2,4-dichloro-6-iodo-3-(tetrahydro-2H-pyran-4-yl)quinoline (0.734 g, 1.80 mmol, Intermediate 6: step c), sodium methoxide (0.972 g, 18.0 mmol), and toluene (18 mL) was stirred at 120° C. under argon for 20 hours. The reaction was cooled to room temperature, filtered, and the filter cake was washed with toluene (2×3 mL). The combined filtrates were concentrated to provide the title compound as a yellow solid.

›EXAMPLES · 4 of 31

Intermediate 7

1-(4-Benzoylpiperidin-1-yl)ethanone

A mixture of phenyl(piperidin-4-yl)methanone hydrochloride (743 mg, 3.29 mmol) in dichloromethane (13.2 mL) and triethylamine (1.10 mL, 7.90 mmol) in an ice bath under argon was treated with Ac 2 O (0.373 mL, 3.95 mmol) dropwise over 1 minute, and the resulting translucent mixture was immediately removed from the ice bath and stirred at room temperature overnight. The reaction was then extracted with 1 M aqueous HCl (8 mL) followed by 1 M aqueous NaOH (8 mL). The organic layer was dried (Na 2 SO 4 ), filtered, and concentrated to provide the title compound as a translucent beige oil that crystallized upon standing.

Intermediate 8

6-Bromo-3-methoxy-2-phenylquinoline

A mixture of KOH pellets (0.775 g, ˜15 w/w % water, ˜11.8 mmol), 2-amino-5-bromobenzaldehyde (2.31 g, 11.6 mmol), and absolute EtOH (36 mL) was treated with 2-methoxy-1-phenylethanone (1.67 mL, 12.1 mmol), and the dark solution with undissolved KOH was stirred at 95° C. for 15 minutes. The dark homogeneous reaction was allowed to cool to room temperature, and within 1 hour crystallization seemed complete. The reaction was then shaken with water (36 mL) and filtered, and the orange-red filter cake was washed with water (36 mL). The filter cake was air-dried at 110° C. to provide the title compound as orange-tan fine crystals.

Intermediate 9: Step a

6-Bromo-2,4-dichloro-3-methylquinoline

A mixture of 4-bromoaniline (77 g, 450 mmol) and 2-methylmalonic acid (53 g, 450 mmol) in phosphorus oxychloride (500 mL) was stirred at 100° C. for 5 hours. Initially, the mixture was a white slurry, which then turned into a homogeneous red solution. The reaction mixture was cooled to room temperature and stirred overnight. Most of the phosphorus oxychloride was removed by rotary evaporation. The thick red residue was slowly poured into ice (1 L). A yellow solid crashed out and was collected by filtration. The collected solids were placed into a 500 mL flask cooled in an ice-water bath. Concentrated aqueous ammonia solution was added until the pH was between 8-9 (by litmus paper test). The resulting suspension was stirred at room temperature for 20 minutes and filtered. The solids were rinsed with water (500 mL) and then collected. The collected solids were dried and then suspended in acetonitrile (500 mL). The suspension was sonicated for 15 minutes at room temperature. The solids were collected by filtration, rinsed with acetonitrile (200 mL), and dried to afford the title compound.

Intermediate 9: Step b

6-Bromo-4-chloro-2-methoxy-3-methylquinoline

Sodium methoxide (151 g, 2.80 mol) was added to a solution of 6-bromo-2,4-dichloro-3-methylquinoline (67.8 g, 233 mmol, Intermediate 9: step a) in toluene (750 mL) with stirring. The mixture was stirred at 100° C. for 2 hours and then cooled to room temperature. Dichloromethane (500 mL) was added and then the mixture was filtered. The filter cake was washed with dichloromethane (200 mL). The filtrate was concentrated to provide a crude solid which was recrystallized from acetonitrile to provide the title compound as a white solid.

Intermediate 10: Step a

6-(Trifluoromethyl)nicotinoyl chloride

To a 1 L 3-neck flask equipped with an overhead stirrer, Claisen adaptor, nitrogen bubbler, 60 mL addition funnel, and thermocouple was added 6-(trifluoromethyl)nicotinic acid (45 g, 235.5 mmol), dichloromethane (540 mL) and DMF (0.910 mL, 11.77 mmol) via syringe. To this solution was added oxalyl chloride (24.51 mL, 282.56 mmol) and the reaction was allowed to stir at ambient temperature overnight. The reaction was then filtered and the clear filtrate was condensed in vacuo to afford the title compound as a brownish semisolid.

Intermediate 10: Step b

N-Methoxy-N-methyl-6-(trifluoromethyl)nicotinamide

To a 1 L 3-neck flask equipped with an overhead stirrer, Claisen adaptor, nitrogen bubbler, 125 mL addition funnel, and thermocouple was added 6-(trifluoromethyl)nicotinoyl chloride (49.3 g, 235.2 mmol, Intermediate 10: step a), dichloromethane (493 mL), and N,O-dimethylhydroxylamine hydrochloride (25.63 g, 258.8 mmol). After the mixture was cooled to 7° C., diisopropylethylamine (90.263 mL, 517.6 mmol) was added such that the addition temperature did not exceed 16° C. After the addition, the reaction was allowed to warm to room temperature. The reaction was then transferred to a separatory funnel and the organic layer was washed with saturated aqueous NaHCO 3 (2×100 mL) followed by water (100 mL) and then dried over sodium sulfate, then filtered. Solvent removal afforded the title compound as a brownish oil.

Intermediate 10: Step c

(1-Methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone

To a 3 L 4-neck flask equipped with an overhead stirrer, nitrogen bubbler, and thermocouple was added 5-bromo-1-methyl-1H-imidazole (47.96 g, 297.9 mmol), followed by THF (537 mL). To this room temperature solution was added isopropylmagnesium chloride/lithium chloride complex [1.3 M in THF] (246.8 mL, 320.8 mmol) (addition temperature maintained between 16.6 and 25° C.) to afford a milky suspension and the reaction was stirred for 60 minutes and then cooled to 5.3° C. in an ice bath. To this mixture was added a solution of N-methoxy-N-methyl-6-(trifluoromethyl)nicotinamide (53.66 g, 229.14 mmol, Intermediate 10: step b) in THF (268.3 mL) (addition temperature between 5.3 and 5.6° C.) to afford an orange mixture. After addition, the reaction was warmed to room temperature over 2 hours. After stirring at room temperature for 18 hours, THF (200 mL) was added and the reaction was stirred for 2 hours. The reaction was then cooled to 4° C. with an ice bath and carefully quenched with 2 N aqueous HCl to pH=7, quenching temperature reached 12° C. The mixture was diluted with ethyl acetate (500 mL), phase split and the organic layer was washed with brine (2×200 mL), dried over sodium sulfate, filtered, and the solvent was removed. Hot ether was added and the suspension was then filtered to afford the title compound as a solid.

›EXAMPLES · 5 of 31

Intermediate 11: Step a

(2,6-Dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A solution of n-butyllithium in hexanes (2.5 M, 22.5 mL, 56.3 mmol) was added dropwise by syringe to a stirring solution of 1-methyl-1H-1,2,3-triazole (5.00 g, 60.2 mmol, prepared according to PCT Int. Appl., 2008098104) in dry tetrahydrofuran (400 mL) at −55° C. The resulting off-white slurry was stirred at −45° C. for 20 minutes, whereupon a solution of 2,6-dimethyl-pyridine-3-carbaldehyde (8.33 g, 61.7 mmol) in dry tetrahydrofuran (10 mL) was added dropwise by syringe. After 5 minutes, the cooling bath was removed and the reaction mixture was allowed to slowly warm. After 45 minutes, saturated aqueous ammonium chloride solution (10 mL) and ethyl acetate (100 mL) were added. The mixture was concentrated by rotary evaporation. The residue was dissolved in ethyl acetate (300 mL). The organic solution was washed with saturated aqueous sodium chloride solution (100 mL, containing excess solid sodium chloride). The aqueous layer was extracted with ethyl acetate (2×100 mL). The organic layers were combined and the combined solution was concentrated. Ether (100 mL) was added to the residue and the mixture was sonicated for 20 minutes during which time a white solid crashed out. The solids were collected by filtration. Ether (100 mL) was added to the collected solids and the mixture sonicated a second time. After 20 minutes, the mixture was filtered and the solids were collected to provide the title compound as a fine powder.

Intermediate 11: Step b

(2,6-Dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone

A mixture containing (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol (9.8 g, 44.9 mmol, Intermediate 11: step a) and manganese dioxide (18.8 g, 184 mmol) in dry 1,4-dioxane (225 mL) was heated to 100° C. with stirring. After 1 hour, the mixture was cooled to 40° C. The cooled mixture was filtered through a 2 cm pad of Celite® rinsing with tetrahydrofuran (100 mL). The filtrate was concentrated in vacuo to provide the title compound as an off-white solid.

Intermediate 12: Step a

(2,4-Dimethylthiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

1-Methyl-1H-1,2,3-triazole was prepared according to the literature reference WO2008/98104. To a 2 L flask containing 1-methyl-1H-1,2,3-triazole (9 g, 108.3 mmol) was added THF (1500 mL) and the solution was cooled to −40° C. To this colorless homogeneous solution was added n-butyllithium (2.5 M in hexanes, 45 mL, 112.5 mmol) dropwise which immediately afforded a dark brown viscous mixture. The mixture was kept between −10 to −20° C. for 60 minutes, then a THF solution of 2,4-dimethylthiazole-5-carbaldehyde (17.2 g, 121.8 mmol in 200 mL THF) was introduced via cannula. Once the aldehyde was added the reaction was allowed to warm to room temperature. After 3 hours, the reaction was quenched by pouring it into a saturated solution of aqueous NH 4 Cl. The aqueous portion was extracted with EtOAc in portions, 7×400 mL. The combined organics were washed with brine, dried over MgSO 4 , filtered and concentrated to dryness to give a brown oil. Chromatography on silica gel (10% acetone-DCM increasing to 50% acetone and increasing to 10% MeOH-DCM) provided the title compound as an amber solid.

Intermediate 12: Step b

(2,4-Dimethylthiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone

To a 500 mL flask containing (2,4-dimethylthiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol (10.5 g, 46.8 mmol, Intermediate 12: step a) was added 1,4-dioxane (400 mL) and the contents were warmed to form a homogeneous solution. Activated MnO 2 (18 g, 207 mmol) was added and the dark brownish mixture was heated to reflux in an aluminum heating mantle under an atmosphere of N 2 . After 1.5 hours, the contents were filtered while still hot through Celite® and rinsed with warm THF. The resulting light orange solution was concentrated and passed through a silica gel column (25% acetone-DCM) to provide the title compound as a light orange solid.

Intermediate 13: Step a

3-(Cyclopropylmethyl)-4-hydroxy-6-iodoquinolin-2(H)-one

A mixture containing 4-hydroxy-6-iodoquinolin-2(1H)-one (2.0 g, 7.0 mmol, Intermediate 4: step a), cyclopropylcarboxaldehyde (0.52 mL, 7.0 mmol), and diethyl 1,4-dihydro-2,6-dimethyl-3,5-pyridinedicarboxylate (1.9 g, 7.0 mmol) in pyridine (23 mL) was heated to 80° C. After 2 hours, the flask was allowed to cool to 23° C. Diethyl ether (30 mL) was added and then the mixture was concentrated, resulting in a solid residue. The solid was suspended in diethyl ether (50 mL) and then sonicated for 5 minutes. The solids were collected by filtration through paper, rinsing with diethyl ether. The washed solids were dried under high vacuum at 50° C. to afford the title compound as a tan solid.

Intermediate 13: Step b

2,4-Dichloro-3-(cyclopropylmethyl)-6-iodoquinoline

A mixture containing 3-(cyclopropylmethyl)-4-hydroxy-6-iodoquinolin-2(H)-one (1.47 g, 4.00 mmol, Intermediate 13: step a) and phosphorus oxychloride (3.0 mL, 32 mmol) in acetonitrile (20 mL) was heated to 100° C. After 3 hours, the flask was allowed to cool to 23° C. and the mixture was concentrated. The residue was dissolved in dichloromethane (100 mL). Ice (50 mL) and water (50 mL) were added sequentially with stirring. Saturated aqueous ammonia solution was added dropwise until the pH was 10 by litmus paper test. The biphasic mixture was stirred for 15 minutes. The layers were separated. The aqueous layer was extracted with dichloromethane (50 mL). The organic layers were combined and the combined solution was dried with sodium sulfate. The dried solution was filtered. Celite® (4 g) was added to the filtrate and the solvent was removed by rotary evaporation to afford a free-flowing powder. The powder was loaded onto a column of silica gel for purification. Elution with hexanes initially, grading to 5% ethyl acetate-hexanes provided the title compound as an off-white solid.

Intermediate 13: Step c

4-Chloro-3-(cyclopropylmethyl)-6-iodo-2-methoxyquinoline

›EXAMPLES · 6 of 31

Sodium methoxide (1.7 g, 31 mmol) was added to a solution of 2,4-dichloro-3-(cyclopropylmethyl)-6-iodoquinoline (1.19 g, 3.15 mmol, Intermediate 13: step b) in toluene (31 mL) with stirring. The mixture was heated to 110° C. After 18 hours, the flask was allowed to cool to 23° C. Dichloromethane (50 mL) was added. The mixture was filtered through Celite®, rinsing with dichloromethane. Celite® (5 g) was added to the filtrate and the solvent was removed by rotary evaporation to afford a free-flowing powder. The powder was loaded onto a column of silica gel for purification. Elution with hexanes initially, grading to 5% ethyl acetate-hexanes provided the title compound as a solid.

Intermediate 14: Step a

(1-Methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol

A solution of isopropylmagnesium chloride/lithium chloride complex (1.3 M in THF, 10.6 mL, 13.8 mmol) was added dropwise by syringe to a solution of 4-bromo-2-(trifluoromethyl)pyridine (3.12 g, 13.8 mmol) in dry THF (50 mL) at 0° C. After 30 minutes, a solution of 1-methyl-1H-imidazole-5-carbaldehyde (1.38 g, 12.5 mmol) in THF (28.5 mL) was added to the Grignard solution by syringe at 0° C. The reaction mixture was warmed to room temperature over 2 hours after which it was quenched with saturated aqueous ammonium chloride solution. The mixture was partitioned between water and ethyl acetate. The separated aqueous phase was further extracted with ethyl acetate and washed with saturated aqueous NaCl solution. The organic phase was dried (MgSO 4 ), filtered, and concentrated. The crude product was purified by flash column chromatography (silica gel, 0-10% MeOH-DCM) to provide the title compound.

Intermediate 14: Step b

(1-Methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone

A heterogeneous mixture of (1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol (0.300 g, 1.16 mmol, Intermediate 14: step a) and manganese dioxide (0.506 g, 5.83 mmol) in 1,4-dioxane (12 mL) was stirred at 100° C. for 1 hour. The reaction mixture was then cooled to room temperature, filtered through Celite®, washed with EtOAc, and concentrated. The organic phase was dried (MgSO 4 ), filtered, and concentrated. The crude product was purified by flash column chromatography (silica gel, 0-100% EtOAc-DCM) to provide the title compound as a white solid.

Intermediate 15

6-Bromo-3-(bromomethyl)-4-chloro-2-methoxyquinoline

A round-bottomed flask was charged with 6-bromo-4-chloro-2-methoxy-3-methylquinoline (2.86 g, 9.98 mmol, Intermediate 9: step b), N-bromosuccinimide (2.84 g, 15.9 mmol), 1,1′-azobis (cyanocyclohexanecarbonitrile) (0.97 g, 3.9 mmol) and the head space was purged with nitrogen for 5 minutes. Deoxygenated CCl 4 (50 mL, deoxygenation was carried out by purging the solvent with argon for 30 minutes) was added to the mixture and heated to 90° C. for 6 hours. The reaction mixture was cooled to room temperature, and silica gel (15 g), DCM and EtOAc were added and the solvents were removed to provide a free-flowing powder. The powder was loaded onto a silica gel column. Elution with hexanes initially, grading to 10% ethyl acetate-hexanes provided the title compound as a white solid.

Intermediate 16

1-((6-Bromo-4-chloro-2-methoxyquinolin-3-yl)methyl)-4-(trifluoromethyl)piperidin-4-ol

To a mixture containing 6-bromo-3-(bromomethyl)-4-chloro-2-methoxyquinoline (1.0 g, 2.7 mmol, Intermediate 15) and 4-(trifluoromethyl)piperidin-4-ol (0.70 g, 4.1 mmol) in dichloromethane (14 mL) was added N,N-diisopropylethylamine (1.5 mL, 8.7 mmol). After 18 hours, dichloromethane (100 mL) was added and the solution was washed with saturated aqueous sodium bicarbonate solution (50 mL). The washed organic solution was dried with sodium sulfate and the dried solution was filtered. Celite® (8 g) was added to the filtrate and the mixture was concentrated to afford a free flowing powder. The powder was loaded onto a silica gel column. Elution with hexanes initially, grading to 20% ethyl acetate-hexanes provided the title compound as a white solid.

Intermediate 17: Step a

6-Bromo-4-chloro-2-methoxy-3-((4-(2,2,2-trifluoroethyl)piperazin-1-yl)methyl)quinoline

To a mixture containing 6-bromo-3-(bromomethyl)-4-chloro-2-methoxyquinoline (0.75 g, 2.1 mmol, Intermediate 15) and 1-(2,2,2-trifluoroethyl)piperazine (0.63 g, 3.1 mmol) in dichloromethane (10 mL) was added N,N-diisopropylethylamine (1.1 mL, 6.4 mmol). After 18 hours, the reaction mixture was concentrated. The residue was dissolved with ethyl acetate (30 mL). The solution was washed sequentially with saturated aqueous sodium bicarbonate solution (10 mL) and saturated aqueous sodium chloride solution (10 mL). The washed organic solution was dried with sodium sulfate and the dried solution was filtered. Celite® (6 g) was added to the filtrate and the mixture was concentrated to afford a free flowing powder. The powder was loaded onto a silica gel column. Elution with hexanes initially, grading to 20% ethyl acetate-hexanes provided the title compound as an off-white solid.

Intermediate 17: Step b

(4-Chloro-2-methoxy-3-((4-(2,2,2-trifluoroethyl)piperazin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone

A solution of n-butyllithium in hexanes (2.5 M, 0.270 mL, 0.680 mmol) was added dropwise by syringe to a dry ice-acetone cooled (−78° C.), stirring solution of 6-bromo-4-chloro-2-methoxy-3-((4-(2,2,2-trifluoroethyl)piperazin-1-yl)methyl)quinoline (300 mg, 0.663 mmol, Intermediate 17: step a) in tetrahydrofuran (5 mL). After 1 minute, a solution of N-methoxy-N,1-dimethyl-1H-1,2,3-triazole-5-carboxamide (170 mg, 0.999 mmol, Intermediate 72) in dry tetrahydrofuran (2 mL) was added dropwise by syringe. After 5 minutes, the flask was removed from the cooling bath. After 5 minutes, the flask was placed into an ice-water bath. After 15 minutes, water (5 mL) and ethyl acetate (25 mL) were added in sequence. The biphasic mixture was partitioned between half-saturated aqueous sodium chloride solution (25 mL) and ethyl acetate (25 mL). The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Celite® (5 g) was added to the filtrate and the mixture was concentrated in vacuo to afford a free-flowing powder. The powder was loaded onto a silica gel column for flash-column chromatography. Elution with 100% dichloromethane initially, grading to 5% methanol-dichloromethane provided the title compound as a white solid.

›EXAMPLES · 7 of 31

Intermediate 18: Step a

2-(tert-Butyl)malonic acid

A mixture of diethyl 2-(tert-butyl)malonate (4.3 g, 20 mmol) and NaOH (3 M aqueous, 20 mL, 60 mmol) in THF (50 mL) was stirred at 30° C. for 2 days. The mixture was cooled to room temperature and concentrated to dryness. Ice was added to the residue and the aqueous was acidified by the addition of 3 N aqueous HCl. The aqueous was extracted with EtOAc three times and the organics combined and washed with water. The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford the title compound as a white solid.

Intermediate 18: Step b

6-Bromo-3-(tert-butyl)-2,4-dichloroquinoline

A mixture of 4-bromoaniline (3.1 g, 18 mmol) and 2-(tert-butyl)malonic acid (2.89 g, 18 mmol, Intermediate 18: step a) in phosphorus oxychloride (20 mL) was stirred at reflux for 3 hours then cooled to 80° C. and stirred overnight. The mixture was cooled to room temperature and most of the phosphorous oxychloride was removed by rotary evaporation. The residue was poured into ice-water and the pH adjusted to ˜pH 9-10 by the addition of saturated aqueous NH 4 OH. The aqueous was extracted with DCM twice, the organics combined and washed with water. Then the organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness. The residue was purified by FCC (10-50% DCM/heptane) to provide the title compound as a white solid.

Intermediate 19: Step a

2-Cyclohexylmalonic acid

A mixture of dimethyl 2-cyclohexylmalonate (5 g, 23.3 mmol) and NaOH (3 M aqueous, 23.3 mL, 70 mmol) in THF (50 mL) was stirred at 30° C. for 2 days. The mixture was cooled to room temperature and concentrated to dryness. Ice was added to the residue and the aqueous was acidified by the addition of 3 N aqueous HCl. The aqueous was extracted with EtOAc three times and the organics combined and washed with water. The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford the title compound as a white solid.

Intermediate 19: Step b

6-Bromo-2,4-dichloro-3-cyclohexylquinoline

A mixture of 4-bromoaniline (3.44 g, 20 mmol) and 2-cyclohexylmalonic acid (3.72 g, 20 mmol, Intermediate 19: step a) in phosphorus oxychloride (20 mL) was stirred at reflux for 3 hours then cooled to 80° C. and stirred overnight. The mixture was cooled to room temperature and most of the phosphorous oxychloride was removed by rotary evaporation. The residue was poured into ice-water and the pH adjusted to ˜pH 9-10 by the addition of saturated aqueous NH 4 OH. The aqueous was extracted with DCM twice, the organics combined and washed with water. Then the organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness. The residue was purified by FCC (20-50% DCM/heptane) to provide the title compound as a white solid.

Intermediate 20: Step a

Isopropyl 2-isopropoxyacetate

To a solution of isopropanol (403 mL, 5.3 mol) was added sodium metal (4.67 g, 203 mmol) portionwise. The mixture was heated to reflux for 2 hours, then ethyl bromoacetate (20 mL, 177 mmol) was added dropwise over 1 hour. The resulting mixture was stirred at reflux for 2.5 hours, then cooled to room temperature and stirred overnight. The mixture was concentrated to dryness and the residue then dissolved in water (250 mL) and extracted with EtOAc (3×125 mL). The organics were combined, washed with cold water (2×150 mL), dried (Na 2 SO 4 ), filtered and concentrated to dryness to provide the title compound as a light yellow oil.

Intermediate 20: Step b

6-Bromo-4-hydroxy-3-isopropoxyquinolin-2(1H)-one

To a solution of methyl 2-amino-5-bromobenzoate (3.74 g, 15.6 mmol) and isopropyl 2-isopropoxyacetate (3 g, 18.7 mmol, Intermediate 20: step a) in THF (109 mL) was added KHMDS (1 M in THF, 46.8 mL, 46.8 mmol) in one portion. The resulting solution was stirred at room temperature for 50 minutes, during which it turned orange. Additional KHMDS (1 M in THF, 15.6 mL, 15.6 mmol) was then added and the solution stirred at room temperature for 1 hour. MeOH (60 mL) was added to the reaction mixture and the solution stirred for 10 minutes. The solution was concentrated to dryness and then the resulting residue was dissolved in water and acidified with 1 N aqueous HCl to ˜pH 3, where a precipitate appeared. The precipitate was filtered off, washed with water and air dried to afford a sticky orange solid. The crude product was purified by FCC (0.5-5% MeOH/DCM) to provide the title compound as a yellow solid.

Intermediate 20: Step c

6-Bromo-2,4-dichloro-3-isopropoxyquinoline

A mixture of 6-bromo-4-hydroxy-3-isopropoxyquinolin-2(1H)-one (3.43 g, 11.5 mmol, Intermediate 20: step b) in POCl 3 (12.8 mL, 138 mmol) was heated to 105° C. for 25 minutes, then the reaction was allowed to cool to room temperature. The solution was diluted with DCM and poured into a mixture of 6 N aqueous KOH (45 mL) and ice and then stirred for 30 minutes. The pH of the mixture was then adjusted to ˜pH 10 by adding more 6 N aqueous KOH. The mixture was poured into a separatory funnel and the layers were separated. The aqueous was further extracted with DCM (140 mL), then the organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford the crude product as a brown solid. The crude material was purified by filtering through a pad of silica with 1% EtOAc/hexanes, then concentrating the filtrate to dryness to provide the title compound as a cream-colored solid.

Intermediate 21: Step a

6-Bromo-3-ethoxy-4-hydroxyquinolin-2(1H)-one

To a solution of methyl 2-amino-5-bromobenzoate (5 g, 20.9 mmol) and ethyl ethoxyacetate (3.46 mL, 25 mmol) in THF (146 mL) was added KHMDS (1 M in THF, 62.6 mL, 62.6 mmol) in one portion. The resulting solution was stirred at room temperature for 50 minutes. KHMDS (1 M in THF, 20.8 mL, 20.8 mmol) was then added and the solution stirred at room temperature for 30 minutes. MeOH (100 mL) was added to the reaction mixture and the solution stirred for 10 minutes. The solution was concentrated to dryness and then the resulting residue was dissolved in water and acidified with 1 N aqueous HCl to ˜pH 3, where a precipitate appeared. The precipitate was filtered off, washed with water and air dried to afford the crude product as a sticky red-orange solid. The crude product was purified by FCC (0.5-5% MeOH/DCM) to provide the title compound as a pink solid.

›EXAMPLES · 8 of 31

Intermediate 21: Step b

6-Bromo-2,4-dichloro-3-ethoxyquinoline

A mixture of 6-bromo-3-ethoxy-4-hydroxyquinolin-2(1H)-one (1.45 g, 5.1 mmol, Intermediate 21: step a) in POCl 3 (5.7 mL, 61.3 mmol) was heated to 105° C. for 5 hours, then the reaction was allowed to cool to room temperature. The solution was diluted with DCM and poured into a mixture of 6 N aqueous KOH (31 mL) and ice and then stirred for 30 minutes (pH>10). The mixture was poured into a separatory funnel and the layers were separated. The aqueous was further extracted with DCM (100 mL), then the organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a brown solid. The crude material was purified by filtering through a pad of silica with 1% EtOAc/hexanes, then concentrating the filtrate to dryness to provide the title compound as a tan solid.

Intermediate 22: Step a

4-Chloro-N-methoxy-N-methylbenzamide

Pyridine (27.6 mL, 343 mmol) was added to N,O-dimethylhydroxylamine hydrochloride (16.7 g, 172 mmol) in DCM (400 mL). 4-Chlorobenzoyl chloride (20 mL, 156 mmol) was then added and the mixture was stirred at room temperature for 3 days. Solids were removed by vacuum filtration, washing with DCM. The filtrate was washed with 1 N aqueous HCl followed by water. The organic phase was dried (Na 2 SO 4 ), filtered, and concentrated, affording the crude title compound as a colorless liquid which was used without purification in the next step.

Intermediate 22: Step b

(4-Chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanone

Ethyl magnesium bromide (3.0 M in diethyl ether, 21.5 mL, 64.4 mmol) was added via syringe over a few minutes to a clear colorless solution of 5-bromo-1-methyl-1H-imidazole (10.4 g, 64.4 mmol) in THF (100 mL) under a nitrogen atmosphere in an ice bath. A white precipitate formed during the addition. The mixture was removed from the ice bath and was stirred for 20 minutes, then was again cooled in an ice bath before addition of 4-chloro-N-methoxy-N-methylbenzamide (10.7 g, 53.6 mmol, Intermediate 22: step a). The resulting white suspension was stirred overnight at room temperature. The reaction was quenched by addition of saturated aqueous NH 4 Cl and diluted with water. The mixture was partially concentrated to remove THF and was diluted with DCM. The mixture was acidified to pH 1 with 1 N aqueous HCl, then neutralized with saturated aqueous NaHCO 3 . The phases were separated and the aqueous phase was further extracted with DCM. The organic extracts were washed with water, then were dried (Na 2 SO 4 ), filtered, and concentrated, affording a white solid. The crude product was triturated with a mixture of EtOAc:heptanes (1:1, 150 mL). The precipitated solid was collected by vacuum filtration, washing with heptanes, to afford the title compound.

Intermediate 23: Step a

Methyl 5-bromo-2-(2-bromoacetamido)benzoate

To a solution of methyl 2-amino-5-bromobenzoate (7 g, 29.2 mmol) in DCM (88 mL) was added diisopropylethylamine (5 mL, 29.2 mmol) and the resulting solution was cooled to −78° C. Then, bromoacetyl bromide (2.6 mL, 29.2 mmol) was added dropwise over 5 minutes and the mixture stirred at −78° C. for 1 hour. The cooling bath was removed and the reaction was allowed to warm to room temperature and then stir at room temperature for 1 hour. The mixture was cooled to −78° C., bromoacetyl bromide (1.3 mL, 14.6 mmol) was added and then the reaction was stirred at room temperature for 1 hour. The mixture was cooled to −78° C., bromoacetyl bromide (0.26 mL, 2.92 mmol) was added and then the reaction was stirred at room temperature for 2 hours. The reaction mixture was poured into a separatory funnel and washed with saturated aqueous NaHCO 3 (75 mL), water (75 mL), and brine (75 mL). The organic layer was dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a green-brown solid. The crude material was purified by FCC (1-100% EtOAc/hexanes) to provide the title compound as a cream-colored solid.

Intermediate 23: Step b

Methyl 5-bromo-2-(2-(2,2,2-trifluoroethoxy)acetamido)benzoate

To a solution of 2,2,2-trifluoroethanol (1.9 mL, 25.6 mmol) in THF (43 mL) was added KHMDS (1 M in THF, 25.6 mL, 25.6 mmol) in one portion and the resulting mixture was stirred at room temperature for 10 minutes. Then, methyl 5-bromo-2-(2-bromoacetamido)benzoate (3 g, 8.55 mmol, Intermediate 23: step a) was added and the reaction stirred at room temperature for 1.25 hours. The mixture was then concentrated to dryness. The residue was dissolved in DCM (75 mL), washed with water (75 mL) followed by brine (75 mL), dried (Na 2 SO 4 ), filtered and concentrated to dryness to provide the title compound as a yellow solid.

Intermediate 23: Step c

6-Bromo-4-hydroxy-3-(2,2,2-trifluoroethoxy)quinolin-2(1H)-one

To a solution of methyl 5-bromo-2-(2-(2,2,2-trifluoroethoxy)acetamido)benzoate (2.06 g, 5.57 mmol, Intermediate 23: step b) in THF (28 mL) was added KHMDS (1 M in THF, 5.6 mL, 5.57 mmol) and the resulting solution was stirred at room temperature for 35 minutes. KHMDS (1 M in THF, 5.6 mL, 5.57 mmol) was added and the mixture stirred at room temperature for 1.5 hours. KHMDS (1 M in THF, 5.6 mL, 5.57 mmol) was added and the mixture stirred at room temperature for an additional hour. MeOH (50 mL) was then added and the reaction stirred at room temperature for 10 minutes, after which it was concentrated to dryness. The residue was dissolved in water and the pH was adjusted to ˜pH 3 by the addition of aqueous 1 N HCl. The mixture was then concentrated to dryness to afford a yellow solid. The crude material was purified by FCC (1-30% EtOAc/DCM) to provide the title compound as a peach solid.

Intermediate 23: Step d

6-Bromo-2,4-dichloro-3-(2,2,2-trifluoroethoxy)quinoline

A mixture of 6-bromo-4-hydroxy-3-(2,2,2-trifluoroethoxy)quinolin-2(1H)-one (807 mg, 2.03 mmol, Intermediate 23: step c) in POCl 3 (2.3 mL, 24.4 mmol) was heated to 105° C. for 2 hours, then the reaction was allowed to cool to room temperature. The solution was diluted with DCM and poured into a mixture of 6 N aqueous KOH and ice and then stirred for 30 minutes. The pH of the mixture was then adjusted to ˜pH 10 by adding more 6 N aqueous KOH. The mixture was poured into a separatory funnel and the layers were separated. The aqueous was further extracted with DCM (75 mL), then the organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a brown solid. The crude material was purified by filtering through a pad of silica with 1% EtOAc/hexanes, then concentrating the filtrate to dryness to provide the title compound as a tan solid.

›EXAMPLES · 9 of 31

Intermediate 24: Step a

6-Bromo-3-(cyclopropylmethoxy)-4-hydroxyquinolin-2(1H)-one

To a solution of cyclopropanemethanol (2.08 mL, 25.6 mmol) in THF (43 mL) was added KHMDS (1 M in THF, 25.6 mL, 25.6 mmol) in one portion and the resulting mixture was stirred at room temperature for 10 minutes. Then, methyl 5-bromo-2-(2-bromoacetamido)benzoate (3 g, 8.55 mmol, Intermediate 23: step a) was added and the reaction stirred at room temperature for 1 hour. KHMDS (1 M in THF, 8.5 mL, 8.5 mmol) was added and the mixture stirred at room temperature for an additional 45 minutes. KHMDS (1 M in THF, 8.5 mL, 8.5 mmol) was added and the mixture stirred at room temperature for an additional 2 hours. MeOH (80 mL) was then added and the reaction stirred at room temperature for 10 minutes, after which it was concentrated to dryness. The residue was dissolved in water and the pH was adjusted to ˜pH 3 by the addition of aqueous 1 N HCl. The slurry was then concentrated to dryness to afford a sticky brown solid. The crude material was purified by FCC (0.5-5% MeOH/DCM) to provide the title compound as a cream-colored solid.

Intermediate 24: Step b

6-Bromo-2,4-dichloro-3-(cyclopropylmethoxy)quinoline

To a mixture of 6-bromo-3-(cyclopropylmethoxy)-4-hydroxyquinolin-2(1H)-one (673 mg, 2.17 mmol, Intermediate 24: step a) in POCl 3 (2.4 mL, 26 mmol) was added diisopropylethylamine (860 μL, 4.99 mmol) dropwise and the resulting solution was heated to 90° C. for 2 hours. The reaction was allowed to cool to room temperature, then the solution was diluted with DCM and poured into a mixture of 6 N aqueous KOH and ice and stirred for 30 minutes. The pH of the mixture was then adjusted to ˜pH 10 by adding more 6 N aqueous KOH. The mixture was poured into a separatory funnel and the layers were separated. The aqueous was further extracted with DCM (35 mL), then the organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a brown solid. The crude material was purified by filtering through a pad of silica with 1% EtOAc/hexanes, then concentrating the filtrate to dryness to provide the title compound as a tan solid.

Intermediate 25

(2,4-Dichloro-3-isopropoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone

To a solution of (2,4-dichloro-3-isopropoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol (115 mg, 0.3 mmol, Example 172) in 1,4-dioxane (4.1 mL) was added manganese dioxide (117 mg, 1.35 mmol). The resulting mixture was stirred at 100° C. for 1.25 hours. The reaction mixture was then filtered through Celite® while still hot, rinsing with warm THF followed by EtOAc. The filtrate was concentrated to dryness to provide the title compound as a white solid.

Intermediate 26

6-Bromo-4-chloro-3-isopropoxy-2-methoxyquinoline

To a mixture of 6-bromo-2,4-dichloro-3-isopropoxyquinoline (1 g, 2.98 mmol, Intermediate 20: step c) in toluene (30 mL) was added NaOMe (1.61 g, 29.8 mmol) and the resulting mixture heated to 100° C. for 22 hours. The mixture was then cooled to room temperature, diluted with DCM and filtered through a pad of Celite®, rinsing the filter cake with DCM. The filtrate was concentrated to dryness to afford the title compound as a white solid.

Intermediate 27

(4-Chloro-3-isopropoxy-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone

The title compound was prepared using (4-chloro-3-isopropoxy-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol (Example 156) in place of (2,4-dichloro-3-isopropoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol using the procedure described for Intermediate 25.

Intermediate 28

(4-Chloro-3-isopropoxy-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)methanone

To a solution of (4-chloro-3-isopropoxy-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)methanol (181 mg, 0.36 mmol, Example 155) in 1,4-dioxane (4.9 mL) was added manganese dioxide (140 mg, 1.61 mmol). The resulting mixture was stirred at 100° C. for 19 hours. Additional manganese dioxide (140 mg, 1.61 mmol) was added and the reaction stirred at 100° C. for 3 hours. Additional manganese dioxide (140 mg, 1.61 mmol) was added and the reaction stirred at 100° C. for 1.5 hours. The reaction mixture was then filtered through Celite® while still hot, rinsing with warm THF followed by EtOAc. The filtrate was concentrated to dryness and then resubjected to the reaction conditions. To a solution of crude material in 1,4-dioxane (4.9 mL) was added manganese dioxide (140 mg, 1.61 mmol). The resulting mixture was stirred at 100° C. for 3 hours. Additional manganese dioxide (140 mg, 1.61 mmol) was added and the reaction stirred at 100° C. for 16 hours. The reaction mixture was then filtered through Celite® while still hot, rinsing with warm THF followed by EtOAc. The filtrate was concentrated to dryness and purified by FCC (0.5-5% MeOH/DCM) to provide the title compound as a clear colorless oil.

Intermediate 29: Step a

Methyl 2-(2-(benzyloxy)acetamido)-5-bromobenzoate

To a solution of methyl-2-amino-5-bromobenzoate (15 g, 62.6 mmol) in DCM (241 mL) at 0° C. was added benzyloxyacetyl chloride (12.5 mL, 75.1 mmol) followed by Et 3 N (20 mL, 144 mmol) dropwise. The resulting white suspension was stirred at room temperature for 3 hours. The mixture was then washed with saturated aqueous NH 4 Cl (200 mL) followed by water (200 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness. The crude solid was triturated with MeOH (90 mL) and dried under vacuum to afford the title compound as a white solid.

Intermediate 29: Step b

3-(Benzyloxy)-6-bromo-4-hydroxyquinolin-2(1H)-one

To a solution of methyl 2-(2-(benzyloxy)acetamido)-5-bromobenzoate (15 g, 39.7 mmol, Intermediate 29: step a) in THF (198 mL) was added KHMDS (1 M in THF, 119 mL, 119 mmol). The resulting solution was stirred at room temperature for 40 minutes and then additional KHMDS (19.8 mL, 19.8 mmol) was added and stirring continued at room temperature for 2 hours. The mixture was quenched with water (225 mL) and the layers were separated. The aqueous layer was acidified with 1 N aqueous HCl to pH 2-3. Some of the title compound precipitated out of solution and was collected by filtration. The aqueous was then extracted with EtOAc (3×200 mL). The organics were combined with the solid collected previously and sonicated. The solution was dried (Na 2 SO 4 ), filtered and concentrated to dryness to provide the title compound as a yellow solid.

›EXAMPLES · 10 of 31

Intermediate 29: Step c

3-(Benzyloxy)-6-bromo-2,4-dichloroquinoline

To a suspension of 3-(benzyloxy)-6-bromo-4-hydroxyquinolin-2(1H)-one (12.4 g, 35.8 mmol, Intermediate 29: step b) in acetonitrile (119 mL) was added POCl 3 (10 mL, 107.5 mmol) followed by 2,6-lutidine (6.26 mL, 53.7 mmol) dropwise. The suspension was heated to 100° C. for 4 hours, then the reaction was allowed to cool to room temperature. The solids were filtered, rinsed with MeOH and dried under vacuum to afford the title compound as a tan solid.

Intermediate 29: Step d

3-(Benzyloxy)-6-bromo-4-chloro-2-methoxyquinoline

To a mixture of 3-(benzyloxy)-6-bromo-2,4-dichloroquinoline (7 g, 18.3 mmol, Intermediate 29: step c) in toluene (183 mL) was added NaOMe (9.87 g, 182.7 mmol) and the resulting mixture heated to 60° C. for 16.5 hours. Additional NaOMe (1.97 g, 36.5 mmol) was added and the mixture stirred for 30 minutes at 60° C. The mixture was then cooled to room temperature, diluted with DCM and stirred for 15 minutes. The mixture was filtered through a pad of Celite®, rinsing the filter cake with DCM followed by THF. The filtrate was concentrated to dryness to afford the title compound as a cream-colored solid.

Intermediate 30

4-Chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methyl)-2-methoxyquinolin-3-ol

To a solution of (3-(benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanol (2.56 g, 4.61 mmol, Example 168) in MeOH (97 mL) was added 10% Pd/C (246 mg, 0.23 mmol). The reaction vessel was evacuated and then placed under an atmosphere of hydrogen for 1.5 hours. The mixture was then flushed with N 2 and filtered through a pad of Celite®. The filtrate was concentrated to dryness, then DCM was added and the solution concentrated to dryness. The resulting solid was dried in the oven. The solid was then purified by FCC (15% MeOH/DCM) to provide the title compound.

Intermediate 31

4-Chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinolin-3-ol

To a solution of (3-(benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol (2.28 g, 4.42 mmol, Example 169) in MeOH (88 mL) was added 10% Pd/C (235 mg, 0.22 mmol). The reaction vessel was evacuated and then placed under an atmosphere of hydrogen for 2.5 hours. The mixture was then flushed with N 2 and filtered through a pad of Celite®. The Celite® was rinsed with MeOH followed by THF and the filtrate was concentrated to dryness. The residue was purified by FCC (0-5% MeOH/DCM) to provide the title compound as a white solid.

Intermediate 32

Methyl 3-(benzyloxy)-4-chloro-2-methoxyquinoline-6-carboxylate

n-BuLi (1.23 M in hexanes, 5.37 mL, 6.6 mmol) was added dropwise to a stirred solution of 3-(benzyloxy)-6-bromo-4-chloro-2-methoxyquinoline (2.5 g, 6.6 mmol, Intermediate 29: step d) in THF (11.5 mL) under nitrogen at −78° C. After an additional minute, a pellet of dry ice was added to the dark solution, and the flask was quickly resealed, evacuated and flushed with nitrogen. After 5 minutes, the resulting yellow solution was removed from the cold bath and stirred under ambient conditions for 15 minutes. The reaction was then transferred to an ice bath and quenched with iodomethane (410 μL, 6.6 mmol) and DMSO (6.6 mL). The reaction was stirred at 0° C. for 5 minutes, and was then rotovapped to remove the THF. The mixture was treated with Na 2 CO 3 (700 mg, 6.6 mmol) and iodomethane (820 μL, 13.2 mmol) and stirred at 40° C. for 30 minutes. The mixture was then diluted with DCM (65 mL), washed with water (2×75 mL), dried (Na 2 SO 4 ), filtered, and concentrated to dryness to provide a yellow solid. The crude material was purified by FCC (1-10% EtOAc/hexanes) to provide the title compound as a white solid.

Intermediate 33

6-(Bis(1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)-4-chloro-2-methoxyquinolin-3-ol

To a solution of [3-(benzyloxy)-4-chloro-2-methoxyquinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol (3.04 g, 5.87 mmol, Example 75) in MeOH (117 mL) was added 10% Pd/C (313 mg, 0.29 mmol). The reaction vessel was evacuated and then placed under an atmosphere of hydrogen for 2.5 hours. The mixture was then flushed with N 2 and filtered through a pad of Celite®. The Celite® was rinsed with MeOH and the filtrate was concentrated to dryness. The residue was purified by FCC (0-15% MeOH/DCM) to give a white amorphous solid. The solid was azeotroped with toluene (4×) and dried under vacuum at 45° C. to afford the title compound as a peach solid (containing a small amount of MeOH).

Intermediate 34: Step a

Ethyl 6-bromo-4-hydroxy-2-oxo-1,2-dihydroquinoline-3-carboxylate

To a solution of methyl 2-amino-5-bromobenzoate (5 g, 20.9 mmol) and diethyl malonate (3.2 mL, 20.9 mmol) in EtOH (24.5 mL) was added NaOEt (21% solution in EtOH, 8.2 mL, 21.9 mmol) dropwise over 2 minutes. The resulting mixture was stirred at room temperature for 30 minutes. The EtOH was then removed under vacuum. The mixture was heated to 140° C. for 16 hours then allowed to cool to room temperature. The solid obtained was washed with diethyl ether then dissolved in water (50 mL) and the insoluble material was filtered off. The filtrate was acidified to ˜pH 2-3 by the addition of 1 N aqueous HCl. A precipitate formed during acidification which was collected by filtration. The solid was washed with water and dried under vacuum to provide the title compound as a cream-colored solid.

Intermediate 34: Step b

Ethyl 6-bromo-2,4-dichloroquinoline-3-carboxylate

A solution of ethyl 6-bromo-4-hydroxy-2-oxo-1,2-dihydroquinoline-3-carboxylate (2.42 g, 7.75 mmol, Intermediate 34: step a) in POCl 3 (7.2 mL, 77.5 mmol) was heated to 110° C. for 1 hour. The reaction was cooled to room temperature and concentrated to dryness. The residue was then dissolved in DCM (35 mL) and poured into ice-water. The resulting mixture was basified to ˜pH 10-11 by the addition of 1 N aqueous NaOH then extracted with EtOAc (2×75 mL). The organics were combined and washed with water (50 mL) followed by brine (50 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow solid.

›EXAMPLES · 11 of 31

Intermediate 34: Step c

Ethyl 6-bromo-4-chloro-2-methoxyquinoline-3-carboxylate

To a mixture of ethyl 6-bromo-2,4-dichloroquinoline-3-carboxylate (1.07 g, 3.07 mmol, Intermediate 34: step b) in toluene (31 mL) was added NaOMe (346 mg, 6.28 mmol) and the resulting mixture stirred at room temperature for 1.75 hours. The mixture was heated to 40° C. and stirred for 2.5 hours. The mixture was then heated to 60° C. and stirred for 1 hour. The reaction was allowed to cool to room temperature. The mixture was diluted with DCM, stirred for 15 minutes, and filtered through a pad of Celite®, rinsing the filter cake with DCM followed by THF. The filtrate was concentrated to dryness to afford a light yellow solid. The crude material was purified by FCC (0-10% EtOAc/hexanes) to provide the title compound as a white solid.

Intermediate 34: Step d

6-Bromo-4-chloro-2-methoxyquinoline-3-carboxylic acid

To a solution of ethyl 6-bromo-4-chloro-2-methoxyquinoline-3-carboxylate (598 mg, 1.74 mmol, Intermediate 34: step c) in THF (14.5 mL), iPrOH (5.8 mL) and water (5.8 mL) was added LiOH (364 mg, 8.68 mmol) and the reaction stirred at room temperature for 1 hour. The reaction was then heated to 45° C. for 1.75 hours and then 65° C. for 46 hours. The mixture was cooled to room temperature and the pH adjusted to ˜pH 2-3 by the addition of 1 N aqueous HCl. The organics were removed under vacuum and then the aqueous was extracted with EtOAc (3×10 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford the title compound as a yellow solid.

Intermediate 34: Step e

(6-Bromo-4-chloro-2-methoxyquinolin-3-yl)(pyrrolidin-1-yl)methanone

A mixture of 6-bromo-4-chloro-2-methoxyquinoline-3-carboxylic acid (945 mg, 2.84 mmol, Intermediate 34: step d), EDCI (832 mg, 4.25 mmol) and HOBt (581 mg, 4.25 mmol) in DMF (28.4 mL) was stirred at room temperature for 15 minutes. Then, pyrrolidine (1.16 mL, 13.9 mmol) was added and the reaction mixture stirred at room temperature for 1.5 hours. The mixture was concentrated to dryness and the residue partitioned between DCM (50 mL) and saturated aqueous NaHCO 3 (50 mL). The layers were separated and the aqueous further extracted with DCM (50 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a light brown solid. The crude material was purified by FCC (0-50% EtOAc/hexanes) to provide the title compound as a white solid.

Intermediate 35: Step a

Methyl 5-iodo-2-(3-methoxy-3-oxopropanamido)benzoate

To a solution of methyl 5-iodoanthranilate (1.5 g, 5.3 mmol) and sodium bicarbonate (892 mg, 10.6 mmol) in DCM (29.5 mL) at 0° C. was added methyl 3-chloro-3-oxopropionate (587 μL, 5.3 mmol) dropwise over 2 minutes. The cream-colored mixture was stirred at 0° C. for 19 hours. The reaction mixture was then diluted with DCM (25 mL), water (25 mL) was added and the biphasic mixture stirred vigorously for 15 minutes. The layers were separated and the aqueous extracted with DCM (25 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a cream-colored solid. The crude material was purified by FCC (0-50% EtOAc/hexanes) to provide the title compound as a cream-colored solid.

Intermediate 35: Step b

Methyl 4-hydroxy-6-iodo-2-oxo-1,2-dihydroquinoline-3-carboxylate

To a solution of methyl 5-iodo-2-(3-methoxy-3-oxopropanamido)benzoate (1.88 g, 4.98 mmol, Intermediate 35: step a) in THF (29.3 mL) was added NaOMe (25% solution in MeOH, 11.4 mL, 49.8 mmol) dropwise over 5 minutes. The resulting thick cream-colored suspension was stirred at room temperature for 1.5 hours. The mixture was then acidified to ˜pH 2-3 by the addition of 1 N aqueous HCl. During the acidification the mixture became a clear solution then solids crashed out at ˜pH 3. The suspension was stirred at room temperature for 15 minutes then filtered, washing the solids with water. The solids were dried under vacuum to provide the title compound as a white solid.

Intermediate 35: Step c

Methyl 2,4-dichloro-6-iodoquinoline-3-carboxylate

A solution of methyl 4-hydroxy-6-iodo-2-oxo-1,2-dihydroquinoline-3-carboxylate (7.31 g, 21.2 mmol, Intermediate 35: step b) in POCl 3 (19.7 mL, 211.8 mmol) was heated to 110° C. for 1 hour. The reaction was cooled to room temperature and concentrated to dryness. The residue was then dissolved in DCM (100 mL) and poured into ice-water. The resulting mixture was basified to ˜pH 10-11 by the addition of 6 N aqueous NaOH then extracted with DCM (2×100 mL). The organics were combined and washed with water (100 mL) followed by brine (100 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a brown solid. The crude material was purified by FCC (0-10% EtOAc/hexanes) to provide the title compound as a light yellow solid.

Intermediate 35: Step d

Methyl 4-chloro-6-iodo-2-methoxyquinoline-3-carboxylate

To a mixture of methyl 2,4-dichloro-6-iodoquinoline-3-carboxylate (250 mg, 0.65 mmol, Intermediate 35: step c) in toluene (6.5 mL) was added NaOMe (74 mg, 1.34 mmol) and the resulting mixture stirred at room temperature for 16 hours. The mixture was diluted with DCM, stirred for 15 minutes, and filtered through a pad of Celite®, rinsing the filter cake with DCM followed by THF. The filtrate was concentrated to dryness to afford a light yellow oil. The crude material was purified by FCC (0-10% EtOAc/hexanes) to provide the title compound as a clear colorless oil.

Intermediate 35: Step e

4-Chloro-6-iodo-2-methoxyquinoline-3-carboxylic acid

To a solution of methyl 4-chloro-6-iodo-2-methoxyquinoline-3-carboxylate (153 mg, 0.41 mmol, Intermediate 35: step d) in THF (3.4 mL), iPrOH (1.35 mL) and water (1.35 mL) was added LiOH (85 mg, 2.03 mmol) and the reaction stirred at room temperature for 70 minutes. The reaction was then heated to 45° C. for 1 hour and then 65° C. for 20 hours. The mixture was cooled to room temperature and the pH adjusted to ˜pH 2-3 by the addition of 1 N aqueous HCl. The organics were removed under vacuum and then the aqueous was extracted with EtOAc (3×10 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford the title compound as a yellow solid.

›EXAMPLES · 12 of 31

Intermediate 35: Step f

(4-Chloro-6-iodo-2-methoxyquinolin-3-yl)(4-(trifluoromethyl)piperidin-1-yl)methanone

To a mixture of 4-chloro-6-iodo-2-methoxyquinoline-3-carboxylic acid (148 mg, 0.39 mmol, Intermediate 35: step e), HOBt (79.2 mg, 0.58 mmol) and triethylamine (107 μL, 0.77 mmol) in DMF (3.9 mL) was added 4-(trifluoromethyl)piperidine-HCl (367 mg, 1.9 mmol). The resulting mixture was stirred at room temperature for 15 minutes, then EDCI (114 mg, 0.58 mmol) was added and the mixture stirred at room temperature for an additional 23 hours. The reaction was concentrated to dryness and the residue partitioned between DCM (15 mL) and saturated aqueous NaHCO 3 (15 mL). The layers were separated and the aqueous extracted with DCM (15 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford an orange solid. The crude material was purified by FCC (0-50% EtOAc/hexanes) to provide the title compound as a white solid.

Intermediate 36

4-Chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methyl)-2-methoxyquinoline-3-carboxylic acid

To a solution of 4-chloro-6-iodo-2-methoxyquinoline-3-carboxylic acid (100 mg, 0.28 mmol, Intermediate 35: step e) and (1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone (88 mg, 0.34 mmol, Intermediate 10: step c) in THF (6.6 mL) at −78° C. was added n-BuLi (1.85 M in hexanes, 320 μL, 0.59 mmol) dropwise. The resulting orange solution was stirred at −78° C. for 15 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (7 mL), water (20 mL) and EtOAc (20 mL) were added and the layers separated. The aqueous layer was acidified to ˜pH 2 by addition of 1 N aqueous HCl, then extracted with EtOAc (20 mL). The EtOAc was back-extracted with water (15 mL). The aqueous layers were combined and basified to ˜pH 5 by addition of 1 N aqueous NaOH and saturated with NaCl. The aqueous was then extracted with 2-methyl-THF (4×25 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford the title compound as a white amorphous solid.

Intermediate 37

4-Chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinoline-3-carboxylic acid

To a solution of 4-chloro-6-iodo-2-methoxyquinoline-3-carboxylic acid (300 mg, 0.83 mmol, Intermediate 35: step e) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (223 mg, 1.03 mmol, Intermediate 11: step b) in THF (9.8 mL) at −78° C. was added n-BuLi (1.85 M in hexanes, 959 μL, 1.77 mmol) dropwise. The resulting yellow solution was stirred at −78° C. for 5 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (20 mL), water (40 mL) and EtOAc (40 mL) were added and the layers separated. The aqueous layer was acidified to ˜pH 2 by addition of 1 N aqueous HCl, then extracted with EtOAc (40 mL). The aqueous layer was then basified to ˜pH 5 by addition of 1 N aqueous NaOH and saturated with NaCl. The aqueous was then extracted with 2-methyl-THF (4×40 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford the title compound as a light yellow solid.

Intermediate 38: Step a

3-((4-Bromophenyl)amino)-3-oxopropanoic acid

A mixture of 4-bromoaniline (241.6 g, 1.41 mol) and 2,2-dimethyl-1,3-dioxane-4,6-dione (203.4 g, 1.41 mol) in a round bottom flask was heated to 80° C. The solids start to melt around 70° C. After 1 hour, the melted solids re-solidified. The mixture was cooled to room temperature, and then ethyl acetate (200 mL) was added. The resulting mixture was heated to 70° C. for 1 hour then cooled to room temperature. Ethyl acetate was removed by evaporation, and the residue was suspended in diethyl ether (300 mL), sonicated and filtered. The filter cake was washed with minimal amounts of diethyl ether and dried under high vacuum to afford the title compound as a white solid.

Intermediate 38: Step b

6-Bromo-4-hydroxyquinolin-2(1H)-one

Procedure A:

A mixture of 3-((4-bromophenyl)amino)-3-oxopropanoic acid (Intermediate 38: step a, 50.0 g, 194 mmol) and Eaton's reagent (100 mL) was stirred at 70° C. for 20 hours, cooled to room temperature, and poured into an ice/water mixture (200 mL). The resulting slurry was sonicated for 30 minutes and filtered. The collected solid was dried under high vacuum, then suspended in ethanol (100 mL) and sonicated. The solid was collected by filtration and dried under high vacuum to afford the title compound as a yellow solid.

Procedure B:

According to the general method described in Synthetic Communications 2010, 40, 732, a mixture of 4-bromoaniline (30.0 g, 174 mmol) and 2,2-dimethyl-1,3-dioxan-4,6-dione (25.1 g, 174 mmol) was heated to 80° C. for 1.5 hours and cooled to ambient temperature to receive 3-((4-bromophenyl)amino)-3-oxopropanoic acid. The acetone byproduct was removed under vacuum to provide the intermediate product as a dry solid. Eaton's reagent (100 mL) was added to the solid, then heated to 70° C. overnight and cooled to room temperature. The mixture was poured into water and the brown precipitate was filtered and rinsed with water. The brown precipitate was triturated with ethanol, then filtered to provide the title compound as a light brown solid.

Intermediate 38: Step c

Tert-Butyl 4-(2-(6-bromo-2,4-dihydroxyquinolin-3-yl)ethyl)piperidine-1-carboxylate

To a solution of 6-bromo-4-hydroxyquinolin-2(1H)-one (1.05 g, 4.37 mmol, Intermediate 38: step b) and tert-butyl 4-(2-oxoethyl)piperidine-1-carboxylate (0.99 g, 4.37 mmol) in pyridine (7.9 mL) was added diethyl 1,4-dihydro-2,6-dimethyl-3,5-pyridinecarboxylate (1.11 g, 4.37 mmol) and the resulting suspension heated to 100° C. for 5 hours. The mixture was cooled to room temperature and diluted with Et 2 O. The ether was decanted and the residue then concentrated to dryness to afford a solid. Diethyl ether was added and the resulting suspension filtered. The solids were dried to provide the title compound.

›EXAMPLES · 13 of 31

Intermediate 38: Step d

6-Bromo-2,4-dichloro-3-(2-(piperidin-4-yl)ethyl)quinoline

To a suspension of tert-butyl 4-(2-(6-bromo-2,4-dihydroxyquinolin-3-yl)ethyl)piperidine-1-carboxylate (1.36 g, 3.01 mmol, Intermediate 38: step c) in acetonitrile (10 mL) was added POCl 3 (0.84 mL, 9.04 mmol) and the mixture heated to 100° C. overnight. The resulting suspension was filtered and MeOH was then added to the solids and the suspension stirred at room temperature for 1 hour. The suspension was filtered and the filtrate concentrated to dryness to afford a solid. The solids were suspended in DCM and the mixture cooled to 0° C. The pH was neutralized by the addition of concentrated aqueous NH 4 OH dropwise. The mixture was then diluted with water and extracted with DCM. The organics were combined, dried (MgSO 4 ), filtered and concentrated to dryness to provide the title compound.

Intermediate 38: Step e

tert-Butyl 4-(2-(6-bromo-2,4-dichloroquinolin-3-yl)ethyl)piperidine-1-carboxylate

To a solution of 6-bromo-2,4-dichloro-3-(2-(piperidin-4-yl)ethyl)quinoline (490 mg, 1.26 mmol, Intermediate 38: step d) in DCM (4.2 mL) was added 4-(dimethylamino)pyridine (31 mg, 0.25 mmol) and Et 3 N (350 μL, 2.52 mmol) followed by di-tert-butyl dicarbonate (331 mg, 1.51 mmol). The resulting mixture was stirred at room temperature for 6 hours. The solution was diluted with DCM and washed with saturated aqueous NaHCO 3 followed by water. The organics were dried (MgSO 4 ), filtered and concentrated to dryness to provide the title compound which was used without further purification.

Intermediate 38: Step f

tert-Butyl 4-(2-(6-bromo-4-chloro-2-methoxyquinolin-3-yl)ethyl)piperidine-1-carboxylate

To a solution of tert-butyl 4-(2-(6-bromo-2,4-dichloroquinolin-3-yl)ethyl)piperidine-1-carboxylate (616 mg, 1.26 mmol, Intermediate 38: step e) in toluene (4 mL) was added NaOMe (341 mg, 6.31 mmol) and the resulting suspension stirred at 110° C. overnight. The mixture was allowed to cool to room temperature and then diluted with EtOAc and washed with water. The organics were dried (MgSO 4 ), filtered and concentrated to dryness. The residue was purified by FCC (0-10% EtOAc/heptane) to provide the title compound.

Intermediate 39

4-Chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methyl)-2-methoxyquinolin-3-yl trifluoromethanesulfonate

To a suspension of 4-chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methyl)-2-methoxyquinolin-3-ol (750 mg, 1.61 mmol, Intermediate 30) in CH 2 Cl 2 (15 mL) was added pyridine (390 μL, 4.84 mmol) and the reaction became a solution. The solution was cooled to 0° C. and trifluoromethanesulfonic anhydride (683 mg, 2.42 mmol) was added dropwise. The reaction was stirred at 0° C. for 1 hour, then the ice bath was removed and stirring continued for an additional hour. Trifluoromethanesulfonic anhydride (683 mg, 2.42 mmol) was then added and the mixture stirred at room temperature for 1 hour. The solution was poured into a mixture of 1 N aqueous HCl (20 mL) and ice and then the aqueous was extracted with CH 2 Cl 2 . The organics were washed with water followed by saturated aqueous NaHCO 3 and brine. The aqueous layers were combined and back-extracted with EtOAc. The EtOAc layers were combined and washed with water followed by saturated aqueous NaHCO 3 and brine. The CH 2 Cl 2 and EtOAc layers were combined, dried (MgSO 4 ), filtered and concentrated to dryness. The crude material was purified by FCC (0-5% MeOH/CH 2 Cl 2 ) to provide the title compound.

Intermediate 40

6-Bromo-4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinoline

Diisopropylethylamine (0.850 mL, 4.93 mmol) was added to a stirring solution of 6-bromo-3-(bromomethyl)-4-chloro-2-methoxyquinoline (0.900 g, 2.46 mmol, Intermediate 15) and 4-(trifluoromethyl)piperidine hydrochloride (0.490 g, 2.60 mmol) in DCM (25 mL). The resulting mixture was stirred for 3 hours at room temperature. DCM was added and the organic layer was washed with a saturated aqueous solution of NaHCO 3 , and a saturated aqueous NaCl solution. The organic phase was dried (MgSO 4 ), filtered, and concentrated to dryness. The crude product was purified by flash column chromatography (silica gel, 0-10% EtOAc-hexanes) to provide the title compound as a white solid.

Intermediate 41

3-((4-(1H-Pyrazol-1-yl)piperidin-1-yl)methyl)-6-bromo-4-chloro-2-methoxyquinoline

The title compound was prepared analogously to the method in Intermediate 40 using 4-(1H-pyrazol-1-yl)piperidine in place of 4-(trifluoromethyl)piperidine hydrochloride.

Intermediate 42

(3-((4-(1H-Pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone

A heterogeneous mixture of (3-((4-(1H-pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol (0.24 g, 0.49 mmol, Example 175) and manganese dioxide (0.217 g, 2.49 mmol) in 1,4-dioxane (5 mL) was stirred at 100° C. for 1 hour. The reaction mixture was then cooled to room temperature, filtered through Celite®, washed with THF, and EtOAc and concentrated to provide the title compound.

Intermediate 43

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone

The title compound was prepared analogously to the method in Intermediate 42 using (4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol (Example 176) in place of (3-((4-(1H-pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol.

Intermediate 44

6-Bromo-4-chloro-3-((4,4-difluoropiperidin-1-yl)methyl)-2-methoxyquinoline

The title compound was prepared analogously to the method in Intermediate 40 using 4,4-difluoropiperidine hydrochloride in place of 4-(trifluoromethyl)piperidine hydrochloride.

Intermediate 45

6-Bromo-4-chloro-3-((4-fluoropiperidin-1-yl)methyl)-2-methoxyquinoline

The title compound was prepared analogously to the method in Intermediate 40 using 4-fluoropiperidine hydrochloride in place of 4-(trifluoromethyl)piperidine hydrochloride.

›EXAMPLES · 14 of 31

Intermediate 46

6-Bromo-4-chloro-3-((3,3-difluoroazetidin-1-yl)methyl)-2-methoxyquinoline

The title compound was prepared analogously to the method in Intermediate 40 using 3,3-difluoroazetidine hydrochloride in place of 4-(trifluoromethyl)piperidine hydrochloride.

Intermediate 47

6-Bromo-4-chloro-3-((3-fluoroazetidin-1-yl)methyl)-2-methoxyquinoline

The title compound was prepared analogously to the method in Intermediate 40 using 3-fluoroazetidine hydrochloride in place of 4-(trifluoromethyl)piperidine hydrochloride.

Intermediate 48

6-Bromo-4-chloro-2-methoxy-3-((2,2,6,6-tetramethylpiperidin-1-yl)methyl)quinoline

The title compound was prepared analogously to the method in Intermediate 40 using 2,2,6,6-tetramethylpiperidine in place of 4-(trifluoromethyl)piperidine hydrochloride.

Intermediate 49

6-Bromo-4-chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinoline

The title compound was prepared analogously to the method in Intermediate 40 using 3-(trifluoromethyl)azetidine hydrochloride in place of 4-(trifluoromethyl)piperidine hydrochloride.

Intermediate 50: Step a

6-Bromo-2,4-dichloroquinoline

DIPEA (62 mL, 360 mmol) was carefully added (fuming observed) to a mixture of 6-bromo-4-hydroxyquinolin-2(1H)-one (43.0 g, 180 mmol, Intermediate 38: step b) and phosphorus oxychloride (250 mL). The mixture was stirred at 90° C. for 5 hours, cooled to room temperature, and slowly poured into ice water (200 mL). The resulting mixture was stirred at 0° C. for 1 hour, basified to pH=8 with saturated NaOH aqueous solution at 0° C. The precipitated solid was collected by filtration and further purified by flash column chromatography (silica gel, petroleum ether:ethyl acetate=5:1) to afford the title compound as a yellow solid.

Intermediate 50: Step b

6-Bromo-4-chloro-2-methoxyquinoline

Sodium methoxide (50.1 g, 928 mmol) was added to a solution of 6-bromo-2,4-dichloroquinoline (32 g, 116 mmol, Intermediate 50: step a) in toluene (200 mL). The mixture was stirred at 120° C. for 2 hours then poured into water (200 mL). The resulting mixture was extracted with ethyl acetate (3×200 mL). The combined organic phase was dried over Na 2 SO 4 and concentrated. The residue was rinsed with minimal amounts of ethyl acetate and filtered to afford the title compound as a yellow solid.

Intermediate 50: Step c

6-Bromo-4-chloro-2-methoxyquinoline-3-carbaldehyde

A 1 L, three-necked, round-bottomed flask equipped with a nitrogen inlet, magnetic stirrer, and rubber septum was charged with tetrahydrofuran (60 mL) and diisopropylamine (58.5 mL, 416 mmol). The reaction mixture was cooled to −78° C. and n-BuLi (2.5 M in hexane, 160 mL, 400 mmol,) was added. The solution was stirred at −78° C. for 10 minutes, warmed to 0° C. and stirred for an additional 30 minutes. This freshly prepared LDA solution was added to a solution of 6-bromo-4-chloro-2-methoxyquinoline (36.0 g, 130 mmol, Intermediate 50: step b) in dry THF (1.6 L) under a nitrogen atmosphere at −78° C. over 30 minutes. The resulting brown reaction mixture was stirred at −78° C. for 2 hours, and then dry DMF (16 mL, 200 mmol) was added. The reaction mixture was stirred at −78° C. for another 3 hours, quenched with saturated NH 4 Cl aqueous solution (400 mL) and extracted with EtOAc (3×300 mL). The combined organic phase was washed with brine (500 mL), dried over Na 2 SO 4 , filtered and concentrated to afford the title compound as a yellow solid, which was directly used without any further purification.

Intermediate 50: Step d

(6-Bromo-4-chloro-2-methoxyquinolin-3-yl)methanol

NaBH 4 (6.08 g, 160 mmol) was added slowly to a solution of 6-bromo-4-chloro-2-methoxyquinoline-3-carbaldehyde (24 g, 80 mmol, Intermediate 50: step c) in methanol (100 mL) at 0° C. The mixture was warmed up to room temperature, stirred for 0.5 hour, and quenched with water (100 mL). The resulting mixture was extracted with ethyl acetate (3×100 mL). The combined organic phase was washed with brine (300 mL), dried over Na 2 SO 4 , concentrated and purified by flash column chromatography (silica gel, petroleum ether:ethyl acetate=5:1) to afford the title compound as a white solid.

Intermediate 50: Step e

6-Bromo-4-chloro-2-methoxy-3-(((triisopropylsilyl)oxy)methyl)quinoline

Chlorotriisopropylsilane (0.57 g, 2.98 mmol) was added to a mixture of (6-bromo-4-chloro-2-methoxyquinolin-3-yl)methanol (0.600 g, 1.98 mmol, Intermediate 50: step d) and imidazole (0.405 g, 5.95 mmol) in DMF (5 mL). The mixture was stirred for 15 minutes at room temperature and then heated to 70° C. for 1 hour. The mixture was cooled to room temperature, water was added and the aqueous layer was extracted with EtOAc, washed with brine, dried (MgSO 4 ) and concentrated to dryness to afford the title compound.

Intermediate 50: Step f

(4-Chloro-2-methoxy-3-(((triisopropylsilyl)oxy)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A solution of n-butyllithium (2.5 M in hexanes, 0.6 mL, 1.51 mmol) was added dropwise by syringe to a solution of 6-bromo-4-chloro-2-methoxy-3-(((triisopropylsilyl)oxy)methyl)quinoline (0.800 g, 1.66 mmol, Intermediate 50: step e) in dry THF (22 mL) at −78° C. After 2 minutes, a solution of (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (0.32 g, 1.51 mmol, Intermediate 11: step b) in dry THF (6 mL) was added dropwise by syringe. An additional 2 mL of THF was used to complete the quantitative addition. After 10 minutes, the flask was removed from the dry-ice bath and placed into an ice-water bath. After 2 hours, the reaction was quenched with saturated aqueous ammonium chloride solution and the mixture was partitioned between water and EtOAc. The layers were separated and the aqueous phase was further extracted with EtOAc and washed with saturated aqueous NaCl solution. The organic phase was dried (MgSO 4 ), filtered, and concentrated to dryness. The crude product was purified by flash column chromatography (silica gel, 0-60% EtOAc/hexanes) to provide the title compound.

›EXAMPLES · 15 of 31

Intermediate 50: Step g

(4-Chloro-3-(hydroxymethyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

TBAF (0.42 mL, 0.42 mmol, 1 M in THF) was added to a solution of (4-chloro-2-methoxy-3-(((triisopropylsilyl)oxy)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol (0.25 g, 0.42 mmol, Intermediate 50: step f) in THF (17 mL). After stirring at room temperature for 3 hours, the mixture was diluted with EtOAc, washed with aqueous saturated NaCl solution, dried (MgSO 4 ), filtered and concentrated. The crude product was purified by flash column chromatography (silica gel, 0-100% EtOAc/DCM) to provide the title compound.

Intermediate 51: Step a

(1-Methyl-1H-1,2,3-triazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol

A solution of n-BuLi (0.73 mL, 1.8 mmol, 2.5 M solution in hexane) was added slowly to a solution of 1-methyl-1H-1,2,3-triazole (0.83 g, 10 mmol) in THF (12 mL) at −50° C. After addition, stirring was continued for an additional 30 minutes and 2-(trifluoromethyl)isonicotinaldehyde (0.350 g, 2.0 mmol,) dissolved in THF (4 mL) was slowly added. An additional 2 mL of THF was used to complete the quantitative addition. The mixture was stirred at −50° C. for 5 minutes then warmed to room temperature and stirred overnight. The solution was quenched with saturated aqueous NH 4 Cl solution. H 2 O was added and layers were separated. The aqueous layer was extracted with EtOAc and the combined organic extracts washed with brine, dried over MgSO 4 , filtered, and evaporated in vacuo. The crude product was purified using flash column chromatography (0 to 50% EtOAc/DCM) to provide the title compound.

Intermediate 51: Step b

(1-Methyl-1H-1,2,3-triazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone

The title compound was prepared analogously to the method in Intermediate 14: step b using (1-methyl-1H-1,2,3-triazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol (Intermediate 51: step a) in place of (1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol.

Intermediate 52

(4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone

The title compound was prepared analogously to the method in Intermediate 42 using (4-chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol (Example 177) in place of (3-((4-(1H-pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol.

Intermediate 53: Step a

N-Methoxy-N-methyltetrahydro-2H-pyran-4-carboxamide

To a solution of tetrahydro-2H-pyran-4-carboxylic acid (5.2 g, 39.9 mmol) in DCM (8.3 mL), CDI (7.12 g, 43.9 mmol) was added and the mixture was stirred for 45 minutes after which N,O-dimethylhydroxylamine hydrochloride (4.29 g, 43.9 mmol) was added and the mixture was stirred for 48 hours. The reaction mixture was quenched with 0.3 M aqueous solution of NaOH and partitioned between water and DCM. The aqueous layer was extracted with DCM, washed with aqueous saturated solution of NaCl, dried (MgSO 4 ) and concentrated. The crude product was used without any further purification.

Intermediate 53: Step b

(1-Methyl-1H-1,2,3-triazol-5-yl)(tetrahydro-2H-pyran-4-yl)methanone

The title compound was prepared analogously to the method in Intermediate 64: step b using N-methoxy-N-methyltetrahydro-2H-pyran-4-carboxamide (Intermediate 53: step a) in place of N-methoxy-N,2,6-trimethylisonicotinamide.

Intermediate 54: Step a

6-Bromo-3-((tetrahydro-2H-thiopyran-4-yl)methyl)quinoline-2,4-diol

The title compound was prepared using tetrahydro-2H-thiopyran-4-carbaldehyde in place of 4,4-difluorocyclohexanecarbaldehyde using the procedure described for Intermediate 56: step a, with the exception that the reaction was carried out at 80° C. for 3.5 hours.

Intermediate 54: Step b

6-Bromo-2,4-dichloro-3-((tetrahydro-2H-thiopyran-4-yl)methyl)quinoline

A heterogeneous mixture of 6-bromo-3-((tetrahydro-2H-thiopyran-4-yl)methyl)quinoline-2,4-diol (3.72 g, 10.5 mmol, Intermediate 54: step a), phosphoryl trichloride (5.5 mL, 59 mmol), and CH 3 CN (25 mL) was stirred at 100° C. for 2.5 hours, during which time it became a clear solution. After cooling down to room temperature, the mixture was concentrated in vacuo. To the residue water was added slowly. The precipitated solid was filtered, washed with water, and dried under air overnight. The solid was dissolved in DCM and purified by FCC (silica gel, 20%-80% DCM in heptanes) to provide the title compound as a yellow solid.

Intermediate 54: Step c

6-Bromo-4-chloro-2-methoxy-3-((tetrahydro-2H-thiopyran-4-yl)methyl)quinoline

The title compound was prepared using 6-bromo-2,4-dichloro-3-((tetrahydro-2H-thiopyran-4-yl)methyl)quinoline (Intermediate 54: step b) in place of 6-bromo-2,4-dichloro-3-((4,4-difluorocyclohexyl)methyl)quinoline (Intermediate 56: step b) using the procedure described for Intermediate 56: step c.

Intermediate 55: Step a

tert-Butyl 3-(hydroxy(1-methyl-1H-1,2,3-triazol-5-yl)methyl)azetidine-1-carboxylate

A 2.5 M solution of n-butyllithium in hexanes (9.60 mL, 24.0 mmol) was added dropwise to a stirring solution of 1-methyl-1H-1,2,3-triazole (2.00 g, 24.0 mmol, prepared according to PCT Int. Appl., 2008098104) in dry THF (100 mL) at −50° C. The reaction became heterogeneous and yellow during addition. After 15 minutes, a solution of tert-butyl 3-formylazetidine-1-carboxylate (4.45 g, 24.0 mmol) in dry THF (10 mL) was added dropwise by syringe. The reaction mixture became homogeneous and was allowed to slowly warm to 0° C. Water (10 mL) and ethyl acetate (100) mL were added. The biphasic mixture was warmed to 23° C. The mixture was partitioned between half-saturated aqueous sodium chloride solution (100 mL) and ethyl acetate (300 mL). The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Celite® (14 g) was added to the filtrate and the solvents were removed by rotary evaporation to provide a free-flowing powder. The powder was loaded onto a silica gel column. Elution with ethyl acetate initially, grading to 5% methanol-ethyl acetate provided the title compound as a white foam.

›EXAMPLES · 16 of 31

Intermediate 55: Step b

tert-Butyl 3-(1-methyl-1H-1,2,3-triazole-5-carbonyl)azetidine-1-carboxylate

Dess-Martin periodinane (10.9 g, 25.7 mmol) was added in one portion to a stirring solution of tert-butyl 3-(hydroxy(1-methyl-1H-1,2,3-triazol-5-yl)methyl)azetidine-1-carboxylate (4.60 g, 17.1 mmol, Intermediate 55: step a) in dry dichloromethane (86 mL). The resulting mixture was stirred at 23° C. After 18 hours, a mixture containing equal parts water, saturated aqueous sodium thiosulfate solution, and saturated aqueous sodium bicarbonate solution was added (200 mL). Dichloromethane (100 mL) was added. The resulting biphasic mixture was stirred for 15 minutes. The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was concentrated. The residue was purified by flash-column chromatography on silica gel eluting with dichloromethane initially, grading to 5% methanol-dichloromethane to provide the title compound as a clear, colorless oil.

Intermediate 56: Step a

6-Bromo-3-((4,4-difluorocyclohexyl)methyl)quinoline-2,4-diol

A mixture of 4,4-difluorocyclohexanecarbaldehyde (1.01 g, 6.84 mmol), 6-bromo-4-hydroxyquinolin-2(1H)-one (1.65 g, 6.85 mmol, Intermediate 38: step b), diethyl 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate (1.82 g, 7.20 mmol), and pyridine (30 mL) was stirred at 80° C. overnight. After removal of most pyridine in vacuo, a solid precipitated. Pyridine (12 mL) and Et 2 O (20 mL) were added and the mixture was stirred for 15 minutes. The white solid was collected by filtration, washed with Et 2 O, and dried to provide the title compound as a white solid.

Intermediate 56: Step b

6-Bromo-2,4-dichloro-3-((4,4-difluorocyclohexyl)methyl)quinoline

A heterogeneous mixture of 6-bromo-3-((4,4-difluorocyclohexyl)methyl)quinoline-2,4-diol (2.98 g, 8.01 mmol, Intermediate 56: step a), phosphoryl trichloride (5.0 mL, 54 mmol), and CH 3 CN (20 mL) was stirred at 100° C. for 2.5 hours, during which time it became a clear solution. After cooling down to room temperature, a white solid precipitated. A small amount of water was added resulting in the dissolution of the solid. After concentration in vacuo, water was added to the residue slowly. The precipitated off-white solid was filtered, washed with water, and dried under air overnight. The solid was dissolved in DCM and purified by FCC (silica gel, 100% DCM) to afford the title compound as a white solid.

Intermediate 56: Step c

6-Bromo-4-chloro-3-((4,4-difluorocyclohexyl)methyl)-2-methoxyquinoline

A heterogeneous mixture of 6-bromo-2,4-dichloro-3-((4,4-difluorocyclohexyl)methyl)quinoline (1.31 g, 3.20 mmol, Intermediate 56: step b) and NaOMe (1.64 g, 30.4 mmol) in toluene (27 mL) was heated at 105° C. for 15 hours. The solvent was evaporated, and the residue was purified by flash column chromatography (silica gel, 40-80% DCM in heptanes) to provide the title compound.

Intermediate 57: Step a

tert-Butyl 3-((1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)azetidine-1-carboxylate

A solution of n-BuLi (2.0 mL, 5 mmol, 2.5 M solution in hexane) was slowly added to a solution of 5-bromo-1,2-dimethyl-1H-imidazole (0.88 g, 5.1 mmol) in THF (35 mL) at −78° C. After addition, stirring was continued for an additional 30 minutes and tert-butyl 3-formylazetidine-1-carboxylate (0.94 g, 5.1 mmol) dissolved in THF (12 mL) was slowly added. An additional 4 mL of THF was used to complete the quantitative addition. The mixture was stirred at −78° C. for 5 minutes then warmed to room temperature and stirred for 1 hour. The solution was quenched with aqueous saturated NH 4 Cl solution and layers were separated. The aqueous layer was extracted with EtOAc and the combined organic extracts washed with brine, dried over MgSO 4 , filtered and evaporated in vacuo. The crude product was purified by triturating with DCM to provide the title compound.

Intermediate 57: Step b

tert-Butyl 3-(1,2-dimethyl-1H-imidazole-5-carbonyl)azetidine-1-carboxylate

A heterogeneous mixture of tert-butyl 3-((1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)azetidine-1-carboxylate (0.39 g, 1.4 mmol, Intermediate 57: step a) and manganese dioxide (0.843 g, 9.70 mmol) in 1,4-dioxane (28 mL) was stirred at 100° C. for 16 hours. The reaction mixture was then cooled to room temperature, filtered through Celite®, washed with THF, DCM, and EtOAc and concentrated to provide the title compound.

Intermediate 58: Step a

tert-Butyl 3-(hydroxy(2-(trifluoromethyl)pyridin-4-yl)methyl)azetidine-1-carboxylate

The title compound was prepared analogously to the method in Intermediate 14: step a using tert-butyl-3-formylazetidine-1-carboxylate in place of 1-methyl-1H-imidazole-5-carbaldehyde.

Intermediate 58: Step b

tert-Butyl 3-(2-(trifluoromethyl)isonicotinoyl)azetidine-1-carboxylate

Dess-Martin periodinane reagent (3.32 g, 7.82 mmol) was added to a solution of tert-butyl 3-(hydroxy(2-(trifluoromethyl)pyridin-4-yl)methyl)azetidine-1-carboxylate (0.520 g, 1.56 mmol, Intermediate 58: step a) in DCM (15.6 mL) at room temperature and the mixture was stirred for 15 hours. The reaction mixture was diluted with 30 mL of DCM and treated with 20 mL of a saturated aqueous solution of NaHCO 3 containing 4 g of Na 2 S 2 O 3 . After 10 minutes of stirring, the mixture was transferred to a separatory funnel and the layers were separated. The aqueous layer was extracted with DCM, and washed with saturated aqueous NaCl solution. The organic phase was dried (MgSO 4 ), filtered, and concentrated. The crude product was purified by flash column chromatography (silica gel, 0-40% EtOAc-DCM) to provide the title compound.

Intermediate 59: Step a

6-Bromo-2-chloro-4-methoxy-3-(2,2,2-trifluoroethyl)quinoline

To a flask containing 6-bromo-2,4-dichloro-3-(2,2,2-trifluoroethyl)quinoline (2.0 g, 5.57 mmol, Intermediate 69: step d) was added MeOH (125 mL) and the suspension needed to be sonicated and warmed to become homogeneous. Then, solid NaOMe (635 mg, 11.4 mmol, 97% purity) was added at room temperature. The mixture was heated to 45° C. for 5 hours, and then at room temperature for 18 hours. The solvent was removed under reduced pressure to yield a white solid. The solid was partitioned between water and CHCl 3 (40 mL), and the aqueous further extracted with CHCl 3 (4×40 mL). The combined organics were washed with brine, dried over MgSO 4 , filtered and concentrated to dryness. The residue was chromatographed on silica gel (90% hexanes-CHCl 3 increasing to 30% hexanes) to afford the title compound as a white solid.

›EXAMPLES · 17 of 31

Intermediate 59: Step b

(2-Chloro-4-methoxy-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol

To a flask containing 6-bromo-2-chloro-4-methoxy-3-(2,2,2-trifluoroethyl)quinoline (505 mg, 1.42 mmol, Intermediate 59: step a) was added THF (15 mL) and the solution was cooled to −78° C. Then, n-BuLi (2.5 M in hexanes, 0.68 mmol, 1.7 mmol) was introduced. After 2 minutes, 1,2-dimethyl-1H-imidazole-5-carbaldehyde (208 mg, 1.68 mmol, in 2 mL THF) was introduced. The reaction temperature was allowed to rise gradually to −30° C. over 20 minutes and then quenched with aqueous NH 4 Cl solution. The aqueous portion was extracted with EtOAc (3×35 mL) and the combined organics were washed with brine, dried over MgSO 4 , filtered and concentrated. The residue was chromatographed on silica gel (1% MeOH-DCM increasing to 10% MeOH) to afford the title compound.

Intermediate 59: Step c

(2-Chloro-4-methoxy-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone

To a flask containing (2-chloro-4-methoxy-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol (350 mg, 0.88 mmol, Intermediate 59: step b) was added THF (25 mL) providing a homogeneous solution. Then, manganese dioxide (300 mg, 3.45 mmol) was added and the reaction mixture was heated to reflux for 1 hour at which time the reaction was complete by TLC. Filter through Celite®, rinse with THF and concentrate to afford the title compound which was pure by NMR and used without further purification.

Intermediate 60: Step a

(1,2-Dimethyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol

The title compound was prepared analogously to the method in Intermediate 14: step a using 1,2-dimethyl-1H-imidazole-5-carbaldehyde in place of 1-methyl-1H-imidazole-5-carbaldehyde.

Intermediate 60: Step b

(1,2-Dimethyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone

The title compound was prepared analogously to the method in Intermediate 57: step b using (1,2-dimethyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol (Intermediate 60: step a) in place of tert-butyl 3-((1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)azetidine-1-carboxylate.

Intermediate 61: Step a

(Tetrahydro-2H-pyran-4-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol

The title compound was prepared analogously to the method in Intermediate 14: step a using tetrahydro-2H-pyran-4-carbaldehyde in place of 1-methyl-1H-imidazole-5-carbaldehyde.

Intermediate 61: Step b

(Tetrahydro-2H-pyran-4-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone

The title compound was prepared analogously to the method in Intermediate 58: step b using (tetrahydro-2H-pyran-4-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol (Intermediate 61: step a) in place of tert-butyl 3-(hydroxy(2-(trifluoromethyl)pyridin-4-yl)methyl)azetidine-1-carboxylate.

Intermediate 62

(1,2-Dimethyl-1H-imidazol-5-yl)(tetrahydro-2H-pyran-4-yl)methanone

A solution of n-BuLi (4.0 mL, 10 mmol, 2.5 M solution in hexane) was slowly added to a solution of 5-bromo-1,2-dimethyl-1H-imidazole (1.77 g, 10.2 mmol) in THF (70 mL) at −78° C. After addition, stirring was continued for an additional 30 minutes and N-methoxy-N-methyltetrahydro-2H-pyran-4-carboxamide (1.76 g, 10.1 mmol, Intermediate 53: step a) dissolved in THF (25 mL) was slowly added. An additional 6 mL of THF was used to complete the quantitative addition. The mixture was stirred at −78° C. for 5 minutes then warmed to room temperature and stirred for 1 hour. The solution was quenched with water and layers were separated. The aqueous layer was extracted with DCM and the combined organic extracts washed with brine, dried over MgSO 4 , filtered and evaporated in vacuo. The crude product was purified using flash column chromatography (0 to 6% MeOH/DCM) to provide the title compound.

Intermediate 63: Step a

tert-Butyl 3-(hydroxy(6-(trifluoromethyl)pyridin-3-yl)methyl)azetidine-1-carboxylate

A solution of isopropylmagnesium chloride (2.0 M in THF, 1.5 mL, 3.0 mmol) was added dropwise by syringe to a solution of 5-bromo-2-(trifluoromethyl)pyridine (0.68 g, 3.0 mmol) in dry THF (12 mL) at 0° C. After 30 minutes, a solution of tert-butyl 3-formylazetidine-1-carboxylate (0.505 g, 2.73 mmol) in THF was added to the Grignard solution by syringe at 0° C. The reaction mixture was warmed to room temperature over 1 hour after which it was quenched with saturated aqueous ammonium chloride solution. The mixture was partitioned between water and ethyl acetate. The separated aqueous phase was further extracted with ethyl acetate and washed with saturated aqueous NaCl solution. The organic phase was dried (MgSO 4 ), filtered, and concentrated. The crude product was purified by flash column chromatography (silica gel, 0-40% EtOAc-DCM) to provide the title compound.

Intermediate 63: Step b

tert-Butyl 3-(6-(trifluoromethyl)nicotinoyl)azetidine-1-carboxylate

The title compound was prepared analogously to the method in Intermediate 57: step b using tert-butyl 3-(hydroxy(6-(trifluoromethyl)pyridin-3-yl)methyl)azetidine-1-carboxylate (Intermediate 63: step a) in place of tert-butyl 3-((1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)azetidine-1-carboxylate.

Intermediate 64: Step a

N-Methoxy-N,2,6-trimethylisonicotinamide

To a solution of 2,6-dimethylisonicotinic acid (1.00 g, 6.61 mmol) in DCM (8.3 mL), CDI (1.18 g, 7.27 mmol) was added and the mixture was stirred for 45 minutes after which N,O-dimethylhydroxylamine hydrochloride (0.71 g, 7.3 mmol) was added and the mixture was stirred for 20 hours. The reaction mixture was quenched with 0.3 M aqueous solution of NaOH and partitioned between water and DCM. The aqueous layer was extracted with DCM, washed with aqueous saturated solution of NaCl, dried (MgSO 4 ) and concentrated. The crude product was purified by flash column chromatography (silica gel, 0-100% EtOAc-DCM) to provide the title compound.

Intermediate 64: Step b

(2,6-Dimethylpyridin-4-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone

A solution of n-BuLi (3.8 mL, 9.5 mmol, 2.5 M solution in hexane) was added slowly to a solution of 1-methyl-1H-1,2,3-triazole (0.83 g, 10 mmol) in THF (48 mL) at −50° C. After addition, stirring was continued for an additional 30 minutes and N-methoxy-N,2,6-trimethylisonicotinamide (0.97 g, 5.0 mmol, Intermediate 64: step a) dissolved in THF (12 mL) was slowly added. An additional 2 mL of THF was used to complete the quantitative addition. The mixture was stirred at −50° C. for 5 minutes then warmed to room temperature and stirred overnight. The solution was quenched with saturated aqueous NH 4 Cl. H 2 O was added and layers were separated. The aqueous layer was extracted with EtOAc and the combined organic extracts washed with brine, dried over MgSO 4 , filtered and evaporated in vacuo. The crude product was purified using flash column chromatography (0 to 100% EtOAc/DCM) to provide the title compound.

›EXAMPLES · 18 of 31

Intermediate 65

(4-Chloro-3-isobutyl-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone

To a flask containing (4-chloro-3-isobutyl-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol (570 mg, 1.52 mmol, Example 158) was added 1,4-dioxane (20 mL) followed by activated MnO 2 (500 mg, 5.75 mmol) and the mixture was heated to 95° C. After 2 hours, the contents were filtered while still hot through Celite® and rinsed with THF and concentrated to dryness to provide the title compound as a white amorphous solid which was used without purification.

Intermediate 66: Step a

2-Isobutylmalonic acid

The mixture of diethyl 2-isobutylmalonate (10.0 g, 46.23 mmol) and NaOH (9.2 g, 231.15 mmol) in EtOH (50 mL) and water (20 mL) was stirred at room temperature overnight. The reaction mixture was poured into water (150 mL), and then a 2 N aqueous HCl solution (25 mL) was added to the mixture. The mixture was stirred at room temperature for 30 minutes and then extracted with EtOAc. The organic layer was dried (Na 2 SO 4 ), filtered and concentrated in vacuo to provide the title compound as a white solid.

Intermediate 66: Step b

6-Bromo-2,4-dichloro-3-isobutylquinoline

To a mixture of 2-isobutylmalonic acid (6.41 g, 40.01 mmol, Intermediate 66: step a) and 4-bromoaniline (6.88 g, 40.01 mmol) was added POCl 3 (150 mL) at 0° C. The mixture was stirred at 90° C. overnight. The mixture was cooled to room temperature, concentrated in vacuo, and the residue was purified by flash chromatography to provide the title compound as a white solid.

Intermediate 66: Step c

6-Bromo-4-chloro-3-isobutyl-2-methoxyquinoline

To a flask containing 6-bromo-2,4-dichloro-3-isobutylquinoline (5 g, 15.0 mmol, Intermediate 66: step b) was added toluene (100 mL) followed by solid NaOMe (9 g, 166.6 mmol) at room temperature. The white suspension was stirred at 110° C. for 48 hours. The reaction mixture was filtered through Celite® while still warm and the filter cake was rinsed with toluene (125 mL). The effluent was concentrated and the crude material was chromatographed on silica gel (90% hexane-DCM increasing to 50% DCM) to provide the title compound initially as a colorless viscous gum. The product was dissolved in Et 2 O and hexane and concentrated to dryness to afford a white solid.

Intermediate 66: Step d

(4-Chloro-3-isobutyl-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)methanone

To a flask containing (4-chloro-3-isobutyl-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)methanol (925 mg, 2.4 mmol, Example 157) was added 1,4-dioxane (75 mL) followed by activated manganese dioxide (800 mg, 9.2 mmol) and the mixture was heated to reflux. After 1 hour, the contents were filtered while still hot through Celite® and the filter cake was rinsed with THF. The filtrate was concentrated and chromatographed on silica gel (100% DCM increasing to 3% MeOH-DCM) to provide the title compound as an off white solid.

Intermediate 67: Step a

6-Bromo-2,4-dichloro-3-isopropylquinoline

A flask fitted with a reflux condenser was charged with POCl 3 at room temperature and then 2-isopropylmalonic acid (10 g, 68.4 mmol) was added followed by 4-bromoaniline (12 g, 69.7 mmol). The heterogeneous mixture was heated in an aluminum mantle to 100° C. which resulted in a light brown homogenous solution after approximately 10 minutes. The reaction mixture was stirred at reflux for 4 hours and then at room temperature for 16 hours. After 20 hours, the excess POCl 3 was removed under reduced pressure. The resulting crude material was then poured onto ice chips (˜500 g) in a 1 L flask pre-cooled to 0° C. DCM (˜200 mL) was added and the mixture was stirred at 0° C. as a solution of 6 M aqueous KOH was added carefully to bring the contents to pH=10. The neutralization process was kept at 0° C. throughout. The organic phase was separated and the aqueous portion was washed with DCM (3×250 mL). The combined organics were washed with brine, dried over Na 2 SO 4 , filtered and concentrated. The crude material was first passed through a silica gel plug (100% DCM) and the effluent was concentrated. Trituration of the resulting material with CH 3 CN provided the title compound as a white solid.

Intermediate 67: Step b

6-Bromo-4-chloro-3-isopropyl-2-methoxyquinoline

To a flask containing 6-bromo-2,4-dichloro-3-isopropylquinoline (12 g, 37.6 mmol, Intermediate 67: step a) was added toluene (300 mL) and to this homogeneous solution at room temperature was added solid NaOMe (18 g, 333.2 mmol). The resulting suspension was stirred at reflux (118° C.) in an aluminum mantle for 8 hours, then at 85° C. for 18 hours. The reaction mixture was then filtered through Celite® while still warm and the filter cake was rinsed with toluene (300 mL). The filtrate was concentrated to dryness to afford the title compound as a white solid.

Intermediate 68: Step a

6-Bromo-2,4-dichloro-3-cyclopentylquinoline

A flask fitted with a reflux condenser and a Drierite® drying tube, was charged with POCl 3 (150 mL) at room temperature. 2-Cyclopentylmalonic acid (10 g, 58.1 mmol) was added followed by 4-bromoaniline (10.3 g, 59.8 mmol). Once the thick heterogeneous mixture was heated to reflux a homogeneous light yellow solution resulted. The reaction mixture was stirred at reflux for 4 hours. The excess POCl 3 was removed under reduced pressure. The resulting crude material was then poured onto ice chips (˜500 g) in a 1 L flask pre-cooled to 0° C. DCM was added (˜200 mL) and the solution was stirred at 0° C. as 10 M aqueous KOH (˜300 mL) was added carefully to pH=9. The neutralization process was kept at 0° C. throughout. The organic phase was separated and the aqueous portion was washed with DCM (3×250 mL). The combined organics were washed with brine, dried over MgSO 4 filtered and concentrated. The crude material was passed through a short column of silica gel (20% toluene-DCM) and the effluent was concentrated. The material was recrystallized from MeOH overnight. The resulting solid was collected by filtration and dried to afford the title compound as an off white solid.

›EXAMPLES · 19 of 31

Intermediate 68: Step b

6-Bromo-4-chloro-3-cyclopentyl-2-methoxyquinoline

To a flask containing 6-bromo-2,4-dichloro-3-cyclopentylquinoline (5 g, 14.5 mmol, Intermediate 68: step a) was added toluene (300 mL) followed by solid NaOMe (6.93 g, 128.3 mmol) at room temperature. The suspension was stirred at reflux for 5 hours and then at 95° C. for 16 hours. The reaction mixture was filtered through Celite® while still warm and the filter cake was rinsed with toluene (300 mL). The filtrate was concentrated to give an off white solid. Chromatography on silica gel (90% hexane-DCM increasing to 70% DCM) provided the title compound as a white solid.

Intermediate 68: Step c

(4-Chloro-3-cyclopentyl-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone

To a flask containing (4-chloro-3-cyclopentyl-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol (500 mg, 1.3 mmol, Example 159) was added THF (25 mL) followed by activated MnO 2 (500 mg, 4.44 mmol) and the mixture was heated to reflux. After 60 minutes, the contents were filtered while still hot through Celite® and rinsed with additional THF and concentrated to afford the title compound.

Intermediate 69: Step a

4,4,4-Trifluorobutanoyl chloride

4,4,4-Trifluorobutyric acid (20.0 g, 137 mmol), dichloromethane (275 mL), and oxalyl chloride (12.4 mL, 145 mmol) were added to a round bottom flask and cooled to 0° C. in an ice water bath. DMF (1.06 mL, 13.7 mmol) was then added and the contents stirred at 0° C. for 15 minutes. The ice bath was then removed and the contents were allowed to warm to room temperature. Gas evolution was noticed immediately after addition of DMF (significant, but not violent) and continued at a moderate pace after the ice bath was removed and the reaction warmed to room temperature and ceased after one and a half hours of stirring. The solution of the title compound was used as is in a subsequent reaction.

Intermediate 69: Step b

Methyl 5-bromo-2-(4,4,4-trifluorobutanamido)benzoate

Methyl 2-amino-5-bromobenzoate (26.0 g, 113 mmol), triethylamine (18.9 mL, 136 mmol), and dichloromethane (400 mL) were combined in a round bottom flask. The contents were cooled to 0° C. in an ice water bath, then a solution of 4,4,4-trifluorobutanoyl chloride in dichloromethane (137 mmol, Intermediate 69: step a) was cannulated into the reaction vessel over approximately 15 minutes. The reaction solution was stirred at 0° C. for one hour, then the ice bath was removed and the contents allowed to warm to room temperature gradually, then stirred at room temperature overnight. Reaction contents were quenched with the addition of saturated, aqueous NH 4 Cl solution then transferred to a separatory funnel with EtOAc dilution. The organic phase was separated and the aqueous layer was extracted twice with additional EtOAc. The combined organic phases were dried over MgSO 4 , filtered and rotovapped to dryness to afford the title compound.

Intermediate 69: Step c

6-Bromo-4-hydroxy-3-(2,2,2-trifluoroethyl)quinolin-2(1H)-one

Methyl 5-bromo-2-(4,4,4-trifluorobutanamido)benzoate (20.04 g, 56.59 mmol, Intermediate 69: step b) and THF (350 mL) were combined in a round bottom flask. Potassium hexamethyldisilazane (KHMDS, 0.5 M in toluene, 340 mL, 170 mmol) was then added over 15 minutes. The contents were stirred for approximately 6 hours yielding a tan heterogeneous mixture. To the reaction mixture was added deionized water (approx. 50 mL) then 1 M aqueous NaOH solution (approximately 100 mL) and the reaction contents were stirred until homogeneous. The solution was then transferred to a separatory funnel and the aqueous phase was separated. The organic phase was extracted with a 0.5 M aqueous NaOH solution and the basic, aqueous layers were combined in a large Erlenmeyer flask. Upon acidification of the combined aqueous layers (to approx. pH 4) with 6 M aqueous HCl solution, a tan precipitate was formed which was collected by cooling the heterogeneous aqueous mixture to 0° C. in an ice-water bath, collecting the precipitate on a Buchner funnel with deionized water rinsing. The precipitate was air-dried then further dried under reduced pressure to provide the title compound.

Intermediate 69: Step d

6-Bromo-2,4-dichloro-3-(2,2,2-trifluoroethyl)quinoline

6-Bromo-4-hydroxy-3-(2,2,2-trifluoroethyl)quinolin-2(1H)-one (15.5 g, 48.1 mmol, Intermediate 69: step c) and POCl 3 (135 mL, 1.44 mol) were combined in a round bottom flask and heated to 100° C. for three hours. The reaction was then cooled and excess phosphorous oxychloride was removed by reduced pressure distillation. The dark crude was taken up into chloroform, cooled to 0° C. in an ice water bath, then any residual phosphorous oxychloride was quenched with dionized water and saturated aqueous NH 4 Cl solution. The solution was then transferred to a separatory funnel where the organic layer was separated and the aqueous layer was extracted once with chloroform. The combined organic phases were dried over MgSO 4 , filtered and solvent removed by reduced pressure distillation. The crude product was purified by flash column chromatography (silica gel, 0-20% hexane/ethyl acetate) to afford the title compound.

Intermediate 69: Step e

6-Bromo-4-chloro-2-methoxy-3-(2,2,2-trifluoroethyl)quinoline

6-Bromo-2,4-dichloro-3-(2,2,2-trifluoroethyl)quinoline (4.93 g, 13.7 mmol, Intermediate 69, step d), toluene (700 mL), MeOH (70 mL) and NaOMe (2.23 g, 41.2 mmol) were combined under nitrogen and heated to 65° C. and maintained at that temperature for 2 days. The reaction contents were then cooled to room temperature and transferred to a separatory funnel with EtOAc and saturated, aqueous ammonium chloride solution. The organic phase was separated then the aqueous was extracted twice with EtOAc. The combined organics were dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude product was purified by flash column chromatography (silica gel, 0-10% DCM/hexanes) to provide the title compound.

Intermediate 70

›EXAMPLES · 20 of 31

(2,4-Dichloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone

To a flask containing (2,4-dichloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanol (505 mg, 1.25 mmol, Example 162) was added 1,4-dioxane (30 mL) followed by manganese dioxide (475 mg, 5.5 mmol) and the mixture was heated to reflux. After 1 hour, the contents were filtered through Celite® and rinsed with THF, and the effluent was concentrated. The crude material was chromatographed on silica gel (1% MeOH-DCM increasing to 5% MeOH) to provide the title compound as a tan solid.

Intermediate 71

Methyl 2,4-dichloro-3-(2,2,2-trifluoroethyl)quinoline-6-carboxylate

6-Bromo-2,4-dichloro-3-(2,2,2-trifluoroethyl)quinoline (1.00 g, 2.79 mmol, Intermediate 69: step d) and THF (15 mL) were combined in a round bottom flask under an N 2 atmosphere and cooled to −78° C. in a dry ice acetone bath. n-BuLi (1.6 M in hexanes, 1.74 mL, 2.79 mmol) was then added dropwise via syringe over approximately 2 minutes and allowed to stir at that temperature for an additional 2 minutes. Several pieces of dry ice were then added to the reaction vessel and the contents were stirred for 5 minutes in the dry ice acetone bath, then the bath was removed and contents allowed to gradually warm to room temperature over approximately 2 hours. The mixture was then re-cooled to 0° C. in an ice water bath followed by addition of methyl iodide (0.52 mL, 8.4 mmol) and sodium carbonate (295 mg, 2.79 mmol) then the ice the bath was removed and the contents were warmed to 40° C. for one hour. DMSO (3 mL) was added and the mixture stirred overnight at 40° C. The mixture was then cooled to room temperature, diluted with water and ethyl acetate then transferred to a separatory funnel. The aqueous layer was separated and the organic layer was washed three times with deionized water. The organic phase was dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude product was purified by flash column chromatography (silica gel, 0-25% hexane/ethyl acetate) to afford the title compound.

Intermediate 72

N-Methoxy-N,1-dimethyl-1H-1,2,3-triazole-5-carboxamide

A solution of 1-methyl-1H-1,2,3-triazole (12.9 g, 155 mmol) in THF (260 mL) was cooled to −45° C. Maintaining a temperature of <−35° C., n-BuLi (62.1 mL, 2.5 M in hexanes, 155 mmol) was added over 10 minutes. The reaction mixture was stirred for 30 minutes with cooling to −45° C. and then treated with a sub-surface stream of CO 2(g) for a period of 2 hours. After flushing the −35° C. slurry with N 2(g) for 5 minutes, thionyl chloride (11.8 mL, 163 mmol) was added. The mixture was allowed to warm to room temperature with stirring over 1.25 hours. Addition of N,O-dimethylhydroxylamine hydrochloride (18.14 g, 186 mmol) and N,N-diisopropylethylamine (68.3 mL, 396 mmol) was followed by stirring for 15 hours. Aqueous sodium carbonate (500 mL, 10 wt %) was then added, and the layers were mixed and separated. The aqueous layer was washed with dichloromethane (250 mL and then 125 mL), and the combined organic layers were dried over MgSO 4 , filtered, and concentrated. The concentrate was taken up in ethyl acetate (225 mL), treated with MgSO 4 , and filtered through a pad of silica gel (115 g). The silica gel pad was washed with additional ethyl acetate (800 mL). The eluent was concentrated to dryness to provide the title compound as a yellow solid.

Intermediate 73: Step a

Methyl 2,4-dichloro-3-methylquinoline-6-carboxylate

A mixture of methyl 4-aminobenzoate (1.0 g, 6.6 mmol) and 2-methylmalonic acid (860 mg, 7.28 mmol) in POCl 3 (10 mL) was stirred at 100° C. for 5 hours. The reaction mixture was initially a white slurry and then turned into a homogeneous red solution. The reaction mixture was cooled to room temperature and stirred overnight. Most of the POCl 3 was removed by evaporation under vacuum. The thick red syrup residue was slowly poured into ice/water (50 mL). A yellow solid that precipitated from the mixture was collected by filtration. The filter cake was placed in 100 mL flask cooled in an ice-water bath. Aqueous ammonia solution (about 20 mL) was added until pH˜8-9. The resulting suspension was stirred at room temperature for 20 minutes and then filtered by suction, rinsed with water (50 mL) and dried. The solid was suspended in CH 3 CN (10 mL), sonicated for 15 minutes at room temperature, filtered, rinsed with CH 3 CN (10 mL) and then dried to provide the title compound as a white solid.

Intermediate 73: Step b

Methyl 4-chloro-2-methoxy-3-methylquinoline-6-carboxylate

Sodium methoxide (1.38 g, 25.6 mmol) was added to a stirred solution of methyl 2,4-dichloro-3-methylquinoline-6-carboxylate (860 mg, 3.2 mmol, Intermediate 73: step a) in toluene (20 mL). The mixture was stirred at 80° C. for 4 hours, cooled to room temperature and diluted with water (50 mL). The mixture was extracted with ethyl acetate (3×20 mL). The organic layers were combined and washed with brine (50 mL), dried over Na 2 SO 4 and concentrated to give the title compound as a white solid.

Intermediate 73: Step c

Methyl 3-(bromomethyl)-4-chloro-2-methoxyquinoline-6-carboxylate

To a stirred solution of methyl 4-chloro-2-methoxy-3-methylquinoline-6-carboxylate (300 mg, 1.13 mmol, Intermediate 73: step b) in CCl 4 (20 mL), NBS (201 mg, 1.13 mmol) and AIBN (18.5 mg, 0.113 mmol) were added sequentially. The reaction mixture was stirred at 80° C. overnight, cooled to room temperature and diluted with DCM (20 mL), and then washed with water (20 mL) and brine (20 mL), dried over Na 2 SO 4 and concentrated to dryness. The residue was purified by silica gel column chromatography (eluting with petroleum ether/ethyl acetate=20:1) to provide the title compound as a white solid.

Intermediate 74: Step a

Methyl 4-chloro-3-((diallylamino)methyl)-2-methoxyquinoline-6-carboxylate

The title compound was prepared analogously to the method described for Intermediate 40 using diallyl amine in place of 4-(trifluoromethyl)piperidine hydrochloride and methyl 3-(bromomethyl)-4-chloro-2-methoxyquinoline-6-carboxylate (Intermediate 73: step c) in place of 6-bromo-3-(bromomethyl)-4-chloro-2-methoxyquinoline.

›EXAMPLES · 21 of 31

Intermediate 74: Step b

(4-Chloro-3-((diallylamino)methyl)-2-methoxyquinolin-6-yl)bis(1,2-dimethyl-1H-imidazol-5-yl)methanol

THF (50 mL) was added to 5-bromo-1,2-dimethyl-1H-imidazole (1.37 g, 7.87 mmol), and the mixture was stirred at room temperature for 10 minutes (a clear solution). The mixture was immersed in a dry-ice bath at −78° C. for 3 minutes and then n-BuLi (3 mL, 7.5 mmol, 2.5 M in THF) was added dropwise slowly. After 20 minutes of stirring, a clear solution of methyl 4-chloro-3-((diallylamino)methyl)-2-methoxyquinoline-6-carboxylate (Intermediate 74: step a, 1.2 g, 3.3 mmol) in THF (25 mL+7 mL to quantitate transfer) was added and the mixture stirred for 2 hours, allowing the reaction to warm up slowly to room temperature. The reaction mixture was quenched with saturated aqueous ammonium chloride solution, and the aqueous layer was extracted with ethyl acetate, washed with brine, dried (MgSO 4 ) and concentrated to dryness. The crude product was purified by FCC (0 to 15% MeOH in DCM) to provide the title compound.

Intermediate 75

6-Bromo-4-chloro-2-methoxy-3-((2-(trifluoromethyl)-5,6-dihydro-[1,2,4]triazolo[1,5-a]pyrazin-7(8H)-yl)methyl)quinoline

The title compound was prepared analogously to the method described for Intermediate 40 using 2-(trifluoromethyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[1,5-a]pyrazine in place of 4-(trifluoromethyl)piperidine hydrochloride.

Intermediate 76

6-Bromo-4-chloro-2-methoxy-3-((3-(trifluoromethyl)-5,6-dihydro-[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl)methyl)quinoline

The title compound was prepared analogously to the method described for Intermediate 40 using 3-(trifluoromethyl)-5,6,7,8-tetrahydro-[1,2,4]triazolo[4,3-a]pyrazine in place of 4-(trifluoromethyl)piperidine hydrochloride.

Intermediate 77

N-((6-Bromo-4-chloro-2-methoxyquinolin-3-yl)methyl)-2,2,2-trifluoro-N-methylethanamine

The title compound was prepared analogously to the method described for Intermediate 40 using 2,2,2-trifluoro-N-methylethanamine in place of 4-(trifluoromethyl)piperidine hydrochloride.

Intermediate 78: Step a

4-Cyano-N-methoxy-N-methylbenzamide

A mixture of 4-cyanobenzoic acid (164.3 g, 1.094 mol) and N,O-dimethylhydroxylamine hydrochloride (106.8 g, 1.094 mol) in 2-methyltetrahydrofuran (1.61 L) was treated with propylphosphonic anhydride (977 mL, 50% in ethyl acetate, 1.642 mol) and then cooled to 15° C. Diisopropylethylamine (377 mL, 2.189 mol) was added and an exotherm to 40° C. was observed. The reaction mixture was stirred at 45° C. for 45 minutes and then cooled to room temperature. Aqueous sodium carbonate (323 g in 3 L total volume) was then added and an exotherm to 32° C. and some off-gassing were observed. Solids were removed by filtration, and the layers were separated. The aqueous layer was washed with ethyl acetate (1 L). The combined organic layers were dried over magnesium sulfate, filtered, and concentrated under reduced pressure to a dark tan solid. The residue was taken up in MTBE (650 mL) at 55° C. and then cooled with stirring. The resulting suspension was cooled to 0° C., and the solids were isolated through filtration and washed with cold 3/1 heptane/MTBE (400 mL), affording the title compound.

Intermediate 78: Step b

4-(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinoline-6-carbonyl)benzonitrile

A solution of n-butyllithium (2.5 M in hexanes, 0.8 mL, 2.0 mmol) was added dropwise by syringe to a solution of 6-bromo-4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinoline (0.964 g, 2.20 mmol, Intermediate 40) in dry deoxygenated THF (14 mL) at −78° C. After 2 minutes, a solution of 4-cyano-N-methoxy-N-methylbenzamide (0.13 g, 0.50 mmol, Intermediate 78: step a) in dry THF (4 mL) was added dropwise by syringe. An additional 2 mL of THF was used to complete the quantitative addition. After 2 hours of stirring at −78° C., the reaction was quenched with saturated aqueous ammonium chloride solution and the mixture was partitioned between water and EtOAc. The layers were separated and the aqueous phase was further extracted with EtOAc and washed with saturated aqueous NaCl solution. The organic phase was dried (MgSO 4 ), filtered, and concentrated to dryness. The crude product was purified by flash column chromatography to provide the title compound.

Intermediate 79: Step a

Methyl 4-chloro-3-(hydroxymethyl)-2-methoxyquinoline-6-carboxylate

To a solution of methyl 3-(bromomethyl)-4-chloro-2-methoxyquinoline-6-carboxylate (16 g, 46.6 mmol, Intermediate 73: step c) in 1,4-dioxane/H 2 O (100 mL/100 mL) in a 500 mL round bottomed flask was added Ag 2 SO 4 (14.4 g, 46.6 mmol). The reaction mixture was stirred at 110° C. for 3 hours, cooled to room temperature and diluted with brine (150 mL). The resulting mixture was stirred for 10 minutes and then extracted with ethyl acetate (5×150 mL). The combined organic phase was washed with H 2 O (3×50 mL) and brine (100 mL), dried over Na 2 SO 4 and concentrated to dryness to provide the title compound as a white solid.

Intermediate 79: Step b

Methyl 3-(((tert-butyldimethylsilyl)oxy)methyl)-4-chloro-2-methoxyquinoline-6-carboxylate

To a solution of tert-butylchlorodimethylsilane (15 g, 100 mmol) in DMF (120 mL) in a 500 mL round bottomed flask was added imidazole (14 g, 200 mmol). The mixture was stirred at 25° C. for 30 minutes and then a solution of methyl 4-chloro-3-(hydroxymethyl)-2-methoxyquinoline-6-carboxylate (28 g, 100 mmol, Intermediate 79: step a) in DMF (80 mL) was added dropwise. After the reaction mixture was stirred at room temperature for 12 hours, it was poured into H 2 O (200 mL) and then extracted with ethyl acetate (5×200 mL). The organic layers were combined and washed with H 2 O (50 mL×3) and brine (100 mL), dried over Na 2 SO 4 and concentrated to dryness to provide the title compound as a white solid.

Intermediate 79: Step c

(3-(((Tert-butyldimethylsilyl)oxy)methyl)-4-chloro-2-methoxyquinolin-6-yl)bis(1,2-dimethyl-1H-imidazol-5-yl)methanol

n-BuLi (12 mL, 26 mmol) was added dropwise over 10 minutes to a solution 5-bromo-1,2-dimethyl-1H-imidazole (5.22 g, 30 mmol) in anhydrous THF (50 mL) in a 250 mL three-necked round bottomed flask at −70° C. under N 2 . After the mixture was stirred at −70° C. under N 2 for 30 minutes, a solution of methyl 3-((tert-butyldimethylsilyloxy)methyl)-4-chloro-2-methoxyquinoline-6-carboxylate (3.95 g, 10 mmol, Intermediate 79: step b) in THF (20 mL) was added dropwise and stirring was continued for 2 hours at −60° C. under N 2 . The reaction was quenched by adding NH 4 Cl aqueous solution (1 N, 20 mL) and then extracted with ethyl acetate (2×150 mL). The organic layers were combined and washed with H 2 O (3×50 mL) and brine (100 mL), dried over Na 2 SO 4 and concentrated to dryness to provide the title compound as a yellow solid.

›EXAMPLES · 22 of 31

Intermediate 79: Step d

(4-Chloro-3-(hydroxymethyl)-2-methoxyquinolin-6-yl)bis(1,2-dimethyl-1H-imidazol-5-yl)methanol

To a solution of (3-(((tert-butyldimethylsilyl)oxy)methyl)-4-chloro-2-methoxyquinolin-6-yl)bis(1,2-dimethyl-1H-imidazol-5-yl)methanol (5.56 g, 1 mmol, Intermediate 79: step c) in methylene chloride (10 mL) in a 100 mL round bottomed flask was added trifluoroacetic acid (20 mL). The reaction mixture was stirred at 25° C. for 12 hours, concentrated to dryness and purified by FCC (silica gel, DCM/MeOH=100/1 to 5/1) to provide the title compound as a white solid.

Intermediate 79: Step e

(4-Chloro-3-(chloromethyl)-2-methoxyquinolin-6-yl)bis(1,2-dimethyl-1H-imidazol-5-yl)methanol

(4-Chloro-3-(hydroxymethyl)-2-methoxyquinolin-6-yl)bis(1,2-dimethyl-1H-imidazol-5-yl)methanol (1.586 g, 3.69 mmol, Intermediate 79: step d) was dissolved in tetrahydrofuran (18 mL). Thionyl chloride (2.6 mL, 35.8 mmol) was added and the mixture was stirred at ambient temperature for 1.5 hours. The suspension was concentrated to dryness and used without purification.

Intermediate 80: Step a

Ethyl 6-bromo-2-cyclopropyl-4-hydroxyquinoline-3-carboxylate

To a 200 mL round bottom flask fitted with an air condenser was added ethyl 3-cyclopropyl-3-oxopropanoate (1.6 g, 10.2 mmol) and DMA (42 mL). Sodium hydride (413 mg, 10.3 mmol) was then added portion wise over 10 minutes. To this mixture was added a solution of 6-bromo-1H-benzo[d][1,3]oxazine-2,3-dione (3 g, 12.4 mmol) in DMA (18 mL). The reaction was then stirred at 120° C. for 2 hours. The reaction was cooled to room temperature followed by removal of most of the solvent under reduced pressure. Then, 200 mL of water was added and the resulting precipitate collected by filtration. The precipitate was washed with water (100 mL). A second crop of precipitate was collected from the filtrate the next day and washed with water (100 mL) to provide the title compound. The crude precipitate was used in the next step without further purification.

Intermediate 80: Step b

Ethyl 6-bromo-4-chloro-2-cyclopropylquinoline-3-carboxylate

In a 100 mL round bottom flask was added ethyl 6-bromo-2-cyclopropyl-4-hydroxyquinoline-3-carboxylate (2.0 g, 5.9 mL, Intermediate 80: step a) and acetonitrile (15 mL). To this solution was added POCl 3 (1.7 mL, 18.3 mmol) and the resulting mixture was stirred at 65° C. for 2 hours. The reaction was cooled to room temperature, added dropwise to a mixture of ice (100 mL) and aqueous ammonia (28-30%, 100 mL). The product was then extracted with ethyl acetate (2×100 mL). The combined organic layers were dried (sodium sulfate) and concentrated in vacuo. The crude residue was purified by silica gel chromatography (20% ethyl acetate/hexanes, 120 g column) to afford the title compound.

Intermediate 80: Step c

(6-Bromo-4-chloro-2-cyclopropylquinolin-3-yl)methanol

In a 100 mL round bottom flask under nitrogen was added ethyl 6-bromo-4-chloro-2-cyclopropylquinoline-3-carboxylate (1.35 g, 3.8 mmol, Intermediate 80: step b), and dichloromethane (15 mL). The solution was cooled to 10° C., and then a 1.0 M solution of DIBAL in DCM (11.4 mL) was added dropwise. The reaction was stirred at 10° C. for 1 hour, and then poured into a 20% solution of aqueous potassium sodium tartrate (150 mL). The mixture was stirred vigorously for 18 hours then filtered through a pad of Celite®. The filtrate was extracted twice with DCM and the combined extracts were washed sequentially with water and brine. The solvent was removed under reduced pressure, residue taken up into ether, filtered and dried to provide the title compound. The crude material was used in the next step without further purification.

Intermediate 80: Step d

6-Bromo-4-chloro-3(chloromethyl)-2-cyclopropylquinoline

To a 200 mL round bottom flask under nitrogen was added (6-bromo-4-chloro-2-cyclopropylquinolin-3-yl)methanol (1.2 g, 3.8 mmol, Intermediate 80: step c) and toluene (25 mL). The flask was cooled to 0° C. then methanesulfonyl chloride (0.63 mL, 8.1 mmol) and N,N-diisopropylethylamine (1.3 mL, 8.1 mmol) were added dropwise. The reaction was allowed to stir at 0° C. for 30 minutes then the ice bath was removed. The reaction was checked after stirring for 1 hour at room temperature by LC/MS which showed about 50% conversion to the desired chloride product with the remainder being sulfonate intermediate. Then, 1 eq. of solid LiCl (161 mg, 3.80 mmol) was added to the reaction mixture and it was allowed to stir overnight. The reaction was then partitioned between saturated aqueous NaHCO 3 (150 mL) and ethyl acetate (150 mL). The aqueous layer was extracted once more with ethyl acetate (100 mL), and then the organic layers were combined and washed with brine. The organic layer was dried (sodium sulfate) and the solvent removed under reduced pressure to provide the title compound. The crude material was used in the next step without additional purification.

Intermediate 80: Step e

6-Bromo-4-chloro-2-cyclopropyl-3-((4-trifluoromethyl)piperidin-1-yl)methyl)quinoline

To a 200 mL round bottom flask was added 6-bromo-4-chloro-3(chloromethyl)-2-cyclopropylquinoline (1.1 g, 3.3 mmol, Intermediate 80: step d), 4-(trifluoromethyl)piperidine hydrochloride (630 mg, 3.3 mmol), N,N-diisopropylethyl amine (1.7 mL, 9.9 mmol) and DCM (32 mL). The reaction was then stirred at room temperature for 4 hours. LC/MS showed about 60% conversion to product so an additional 0.25 eq. of amine and base were added (157 mg, 0.830 mmol) and the reaction allowed to stir overnight at room temperature. The reaction was poured into saturated aqueous sodium bicarbonate (150 mL) and extracted with DCM (2×100 mL). The combined organic layers were dried (sodium sulfate) and concentrated in vacuo. The crude product was purified by silica gel chromatography eluting with (25% ethyl acetate/hexanes) to afford the title compound.

Intermediate 81

4-(4-Chloro-2-methoxy-3-(2,2,2-trifluoroethyl)quinoline-6-carbonyl)benzonitrile

To a 25 mL 2-necked flask containing 6-bromo-4-chloro-2-methoxy-3-(2,2,2-trifluoroethyl)quinoline (200 mg, 0.560 mmol, Intermediate 69: step e) was added THF (12 mL) and the solution was cooled to −78° C. Then, n-BuLi (2.5 M in hexanes, 0.25 mL, 0.63 mmol) was added drop wise which resulted in a dark brown mixture. After 2 minutes, 4-cyano-N-methoxy-N-methylbenzamide (155 mg, 0.79 mmol in 2 mL THF, Intermediate 78: step a) was introduced and the solution became a dark green color. After 10 minutes, the reaction mixture was placed in an ice-water bath and the solution changed to a light yellow color. The mixture was stirred at 0° C. for 20 minutes, then at room temperature for 15 minutes at which time the mixture was quenched with saturated aqueous NH 4 Cl solution. The aqueous portion was extracted with EtOAc (3×40 mL) and the combined organics were washed with brine, dried over MgSO 4 , filtered and concentrated to give an amber gum. Chromatography on silica gel (50% hexanes-DCM increasing to 10% hexanes-DCM) afforded the title compound as a white solid.

›EXAMPLES · 23 of 31

Intermediate 82

4-Chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methyl)-2-methoxyquinolin-3-ol

The title compound was prepared using (3-(benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol (Example 228) in place of (3-(benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol using the procedure described for Intermediate 31.

Intermediate 83: Step a

4-(3-(Benzyloxy)-4-chloro-2-methoxyquinoline-6-carbonyl)benzonitrile

A solution of n-butyllithium in hexanes (1.6 M, 0.91 mL, 1.46 mmol) was added dropwise by syringe to a dry ice-acetone cooled (−78° C.), stirring solution of 3-(benzyloxy)-6-bromo-4-chloro-2-methoxyquinoline (500 mg, 1.32 mmol, Intermediate 29: step d) in tetrahydrofuran (18 mL). After 5 minutes, a solution of 4-cyano-N-methoxy-N-methylbenzamide (351 mg, 1.85 mmol, Intermediate 78: step a) in dry tetrahydrofuran (8 mL) was added dropwise by syringe. The flask was rinsed with THF (2 mL), which was also added to the reaction. After 15 minutes, the flask was removed from the dry-ice-acetone bath and placed into an ice-water bath for 30 minutes. Then, the ice-water bath was removed and the mixture was allowed to stir at room temperature for 15 minutes. Then, saturated NH 4 Cl solution was added followed by water (35 mL) and EtOAc (35 mL) and the layers separated. The aqueous was further extracted with EtOAc (2×30 mL). The organic layers were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness. The residue was purified by FCC (MeOH/CH 2 Cl 2 ) to provide the title compound as a cream-colored amorphous solid.

Intermediate 83: Step b

4-((4-Chloro-3-hydroxy-2-methoxyquinolin-6-yl)(hydroxy)(1-methyl-1H-imidazol-5-yl)methyl)benzonitrile

The title compound was prepared using 4-((3-(benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(hydroxy)(1-methyl-1H-imidazol-5-yl)methyl)benzonitrile (Example 229) in place of (3-(benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol using the procedure described for Intermediate 31.

Intermediate 84

4-Chloro-6-((4-chlorophenyl)(hydroxy)(1-methyl-1H-imidazol-5-yl)methyl)-2-methoxyquinolin-3-ol

The title compound was prepared using (3-(benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol (Example 230) in place of (3-(benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol using the procedure described for Intermediate 31.

Intermediate 85

tert-Butyl 3-((4-chloro-2-ethyl-3-hydroxyquinolin-6-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)azetidine-1-carboxylate

To an oven-dried round-bottom flask was added tert-butyl 3-((3-(benzyloxy)-2,4-dichloroquinolin-6-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)azetidine-1-carboxylate (1.2 g, 2.09 mmol, Example 232), PdCl 2 (dppf) (153 mg, 0.21 mmol) and THF (21 mL). The mixture was sparged with nitrogen for 20 minutes then Et 2 Zn (15 wt % in toluene, 2.26 mL, 2.51 mmol) was added. The resulting mixture was stirred at 65° C. for 50 minutes and additional Et 2 Zn (15 wt % in toluene, 2.26 mL, 2.51 mmol) was added and stirring was continued at 65° C. for 50 minutes. The starting material still remained when monitored by LCMS, therefore additional Et 2 Zn (15 wt % in toluene, 2.26 mL, 2.51 mmol) was added and stirring was continued at 65° C. for 60 minutes. The mixture was allowed to cool to room temperature and saturated aqueous NH 4 Cl (50 mL) was added slowly to quench the reaction. The aqueous layer was then extracted with EtOAc (3×40 mL). The organic layers were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness. The residue was purified by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to provide the title compound as a light yellow amorphous solid.

Intermediate 86: Step a

6-Iodo-3-(3,3,3-trifluoropropyl)quinoline-2,4-diol

6-Iodoquinoline-2,4-diol (8.0 g, 28 mmol), 3,3,3-trifluoropropanal (7.1 g, 6 mmol) and diethyl 1,4-dihydro-2,6-dimethy-3,5 pyridinedicarboxylate (7.06 g, 27.9 mmol) were combined in the reaction vessel under N 2 followed by the addition of pyridine (186 mL, 27.9 mmol). The contents were then heated to 60° C. and maintained at that temperature for 2 days. The reaction was cooled to room temperature and the solvent was removed under reduced pressure. The contents were taken up into EtOAc then extracted with 10% aqueous HCl then a saturated, aqueous sodium chloride solution. The organic phase was then separated, dried over magnesium sulfate, filtered and concentrated under reduced pressure to afford the title compound.

Intermediate 86: Step b

2,4-Dichloro-6-iodo-3-(3,3,3-trifluoropropyl)quinoline

6-Iodo-3-(3,3,3-trifluoropropyl)quinoline-2,4-diol (5.0 g, 13 mmol, Intermediate 86: step a), acetonitrile (100 mL) and phosphorous oxychloride (3.7 mL, 39 mmol) were combined in a reaction vessel under N 2 and heated to 80° C. for two hours. Additional phosphorous oxychloride (3.7 mL, 39 mmol) was then added and the contents were re-heated to 80° C. for 4 hours, then the contents were allowed to gradually cool to room temperature and stir overnight. Additional phosphorous oxychloride (3.7 mL, 39 mmol) was then added and the contents were re-heated to 80° C. for approximately 6 hours. The contents were cooled to room temperature, then solvent and excess phosphorous oxychloride were removed under reduced pressure. The crude material was then taken up into chloroform and any remaining phosphorous oxychloride was quenched by the addition of saturated, aqueous ammonium chloride, deionized water and methanol. The contents were transferred to a separatory funnel with chloroform and the organic phase was separated and the aqueous was extracted twice with chloroform. The combined organic layers were dried over magnesium sulfate, filtered and concentrated under reduced pressure. The crude product was purified by flash column chromatography (silica gel, 0-10% hexanes/ethyl acetate) to provide the title compound.

›EXAMPLES · 24 of 31

Intermediate 86: Step c

4-Chloro-6-iodo-2-methoxy-3-(3,3,3-trifluoropropyl)quinoline

To a reaction vessel under nitrogen was added 2,4-dichloro-6-iodo-3-(3,3,3-trifluoropropyl)quinoline (5.01 g, 11.9 mmol, Intermediate 86: step b), toluene (600 mL) and MeOH (60 mL, 11.9 mmol) followed by NaOMe (1.93 g, 35.8 mmol). The contents were then heated to 65° C. and stirred at that temperature overnight, then cooled to room temperature. The reaction contents were then transferred to a separatory funnel with EtOAc and NH 4 Cl (saturated, aqueous solution). The organic phase was separated then the aqueous was extracted twice with EtOAc. The combined organics were dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude product was purified by flash column chromatography (silica gel, 0-50% hexanes/DCM) to afford the title compound.

Intermediate 87: Step a

(3-(Benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(1,3,5-trimethyl-1H-pyrazol-4-yl)methanol

n-BuLi (2.1 mL, 2.5 M in hexanes, 5.2 mmol) was added drop-wise to a −50° C. solution consisting of 3-(benzyloxy)-6-bromo-4-chloro-2-methoxyquinoline (2.0 g, 5.3 mmol, Intermediate 29: step d) and THF (50 mL). The resultant reaction mixture was stirred at −50° C. for 20 minutes and then treated with a solution of 1,3,5-trimethyl-1H-pyrazole-4-carbaldehyde (730 mg, 5.28 mmol) and THF (15 mL) at −50° C. The resulting mixture was stirred at room temperature for 20 minutes before quenching with saturated aqueous NH 4 Cl (20 mL) and extracting with dichloromethane:methanol (5:1, 50 mL×10). The combined organic extracts were dried over Na 2 SO 4 , filtered and concentrated to dryness under reduced pressure to give the crude product, which was purified by FCC (silica gel, eluent: petroleum ether:ethyl acetate=5:1 to 1:1) to afford the title compound.

Intermediate 87: Step b

(3-(Benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(1,3,5-trimethyl-1H-pyrazol-4-yl)methanone

(3-(Benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(1,3,5-trimethyl-1H-pyrazol-4-yl)methanol (1.5 g, 3.4 mmol, Intermediate 87: step a), MnO 2 (3.0 g, 34 mmol) and dichloromethane (50 mL) were added to a 100 mL round-bottomed flask. The reaction mixture was stirred at room temperature for 16 hours. The suspension was filtered through a pad of Celite® and the pad was washed with dichloromethane (100 mL). The filtrate was concentrated to dryness under reduced pressure to give the crude product, which was purified by FCC (silica gel, eluent: petroleum ether:ethyl acetate=5:1 to 1:1) to afford the title compound.

Intermediate 88

4-Chloro-6-((1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)(1,3,5-trimethyl-1H-pyrazol-4-yl)methyl)-2-methoxyquinolin-3-ol

(3-(Benzyloxy)-4-chloro-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1,3,5-trimethyl-1H-pyrazol-4-yl)methanol (410 mg, 0.771 mmol, Example 246), methanol (20 mL) and wet Pd/C (40 mg, 10 wt. %) were added to a 250 mL round-bottomed flask. The resultant reaction mixture was stirred under H 2 (1 atm, balloon) at room temperature for 0.5 hours. The suspension was filtered through a pad of Celite® and the pad was washed with methanol (50 mL). The filtrate was concentrated to dryness under reduced pressure to afford the title compound.

Intermediate 89: Step a

Methyl 2-amino-3-methylbenzoate Methyl 3-methyl-2-nitrobenzoate (45.0 g, 230 mmol), methanol (1 L) and Raney Ni (5 g) were added to a 2 L round-bottomed flask. The resultant reaction mixture was stirred under H 2 (1 atm, balloon) at room temperature for 16 hours. The suspension was filtered through a pad of Celite® and the pad was washed with methanol (500 mL). The filtrate was concentrated to dryness under reduced pressure to afford the title compound.

Intermediate 89: Step b

Methyl 2-amino-5-bromo-3-methylbenzoate

N-Bromosuccinimide (38.5 g, 216 mmol) was added to a mixture consisting of methyl 2-amino-3-methylbenzoate (32.4 g, 196 mmol, Intermediate 89: step a) and dichloromethane (300 mL). The resultant reaction mixture was stirred at room temperature for 16 hours before it was poured into water (200 mL) and the aqueous phase was extracted with dichloromethane (200 mL×3). The combined organic extracts were dried over Na 2 SO 4 , filtered and concentrated to dryness under reduced pressure to afford the title compound.

Intermediate 89: Step c

Methyl 2-(2-(benzyloxy)acetamido)-5-bromo-3-methylbenzoate

Et 3 N (25.5 mL, 183 mmol) was added dropwise to a 0° C. (ice/water bath) solution consisting of methyl 2-amino-5-bromo-3-methylbenzoate (15 g, 61 mmol, Intermediate 89: step b), 2-(benzyloxy)acetyl chloride (15 mL, 95 mmol) and dichloromethane (200 mL). The resulting mixture was stirred at room temperature for 16 hours before pouring it into saturated aqueous NH 4 Cl (200 mL). The aqueous phase was extracted with dichloromethane (200 mL×2). The combined organic extracts were dried over Na 2 SO 4 , filtered and concentrated to dryness under reduced pressure to give the crude product, which was purified by FCC (eluent: petroleum ether:ethyl acetate=5:1) to afford the title compound.

Intermediate 89: Step d

3-(Benzyloxy)-6-bromo-4-hydroxy-8-methylquinolin-2(1H)-one

KHMDS (176 mL, 1 M in THF, 176 mmol) was added dropwise to a 50° C. solution consisting of methyl 2-(2-(benzyloxy)acetamido)-5-bromo-3-methylbenzoate (23 g, 59 mmol, Intermediate 89: step c) and toluene (350 mL). The resultant reaction mixture was stirred at 50° C. for 0.5 hours before it was cooled to room temperature, poured into aqueous 1 N HCl (300 mL) and the aqueous phase was extracted with ethyl acetate (500 mL×3). The combined organic extracts were dried over Na 2 SO 4 , filtered and concentrated to dryness under reduced pressure to afford the title compound.

Intermediate 89: Step e

3-(Benzyloxy)-6-Bromo-2,4-Dichloro-8-Methylquinoline

POCl 3 (25.5 g, 166 mmol) and 2,6-lutidine (9.7 mL, 83 mmol) was added dropwise to a solution consisting of 3-(benzyloxy)-6-bromo-4-hydroxy-8-methylquinolin-2(1H)-one (20.0 g, 55.5 mmol, Intermediate 89: step d) and ACN (350 mL). The resultant mixture was stirred at 100° C. for 8 hours before it was cooled to room temperature, poured into water (300 mL), and the aqueous phase was extracted with dichloromethane (600 mL×2). The combined organic extracts were dried over Na 2 SO 4 , filtered and concentrated to dryness under reduced pressure to give the crude title product, which was purified by FCC (eluent: petroleum ether:ethyl acetate=20:1) to afford the title compound.

›EXAMPLES · 25 of 31

Intermediate 89: Step f

3-(Benzyloxy)-6-bromo-4-chloro-2-methoxy-8-methylquinoline

3-(Benzyloxy)-6-bromo-2,4-dichloro-8-methylquinoline (4.0 g, 10 mmol, Intermediate 89: step e), sodium methoxide (5.40 g, 100 mmol) and toluene (100 mL) were added to a 250 mL round-bottomed flask. The reaction mixture was stirred at 70° C. for 16 hours. After cooling to room temperature, the mixture was diluted with dichloromethane (150 mL), filtered through a pad of Celite® and the pad was washed with THF (30 mL×3). The filtrate was concentrated to dryness under reduced pressure to give the crude title product, which was purified by FCC (silica gel, eluent: petroleum ether:ethyl acetate=5:1 to 1:1) to afford the title compound.

Intermediate 89: Step g

(3-(Benzyloxy)-4-chloro-2-methoxy-8-methylquinolin-6-yl)((1,3,5-trimethyl-1H-pyrazol-4-yl)methanol

n-BuLi (1.02 mL, 2.5 M in hexane, 2.55 mmol) was added drop-wise to a −70° C. solution consisting of 3-(benzyloxy)-6-bromo-4-chloro-2-methoxy-8-methylquinoline (1.0 g, 2.5 mmol, Intermediate 89: step f) and THF (50 mL). The resultant reaction mixture was stirred at −70° C. for 20 minutes and then treated with a solution of 1,3,5-trimethyl-1H-pyrazole-4-carbaldehyde (352 mg, 2.55 mmol) and THF (15 mL) at −70° C. The resulting mixture was stirred at room temperature for 20 minutes before quenching with saturated aqueous NH 4 Cl (20 mL) and extracting with dichloromethane:methanol (5:1, 50 mL×10). The combined organic extracts were dried over Na 2 SO 4 , filtered and concentrated to dryness under reduced pressure to give the crude product, which was purified by FCC (silica gel, eluent: petroleum ether:ethyl acetate=5:1 to 1:1) to afford the title compound.

Intermediate 89: Step h

(3-(Benzyloxy)-4-chloro-2-methoxy-8-methylquinolin-6-yl)((1,3,5-trimethyl-1H-pyrazol-4-yl)methanone

(3-(Benzyloxy)-4-chloro-2-methoxy-8-methylquinolin-6-yl)(1,3,5-trimethyl-1H-pyrazol-4-yl)methanol (2.0 g, 4.4 mmol, Intermediate 89: step g), MnO 2 (3.8 g, 44 mmol) and dichloromethane (100 mL) were added to a 250 mL round-bottomed flask. The reaction mixture was stirred at room temperature for 16 hours. The suspension was filtered through a pad of Celite® and the pad was washed with dichloromethane (100 mL). The filtrate was concentrated to dryness under reduced pressure to afford the title compound.

Intermediate 90

4-Chloro-6-((1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)(1,3,5-trimethyl-1H-pyrazol-4-yl)methyl)-2-methoxy-8-methylquinolin-3-ol

(3-(Benzyloxy)-4-chloro-2-methoxy-8-methylquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1,3,5-trimethyl-1H-pyrazol-4-yl)methanol (600 mg, 1.10 mmol, Example 247), MeOH (50 mL) and wet Pd/C (60 mg, 10 wt. %) were added to a 100 mL round-bottomed flask. The resultant reaction mixture was stirred under H 2 (1 atm, balloon) at room temperature for 0.5 hours. The suspension was filtered through a pad of Celite® and the pad was washed with methanol (50 mL). The filtrate was concentrated to dryness under reduced pressure to afford the title product.

Example 1a: (4-Chloro-3-((4,4-difluorocyclohexyl)methyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A solution of 6-bromo-4-chloro-3-((4,4-difluorocyclohexyl)methyl)-2-methoxyquinoline (1.27 g, 3.14 mmol, Intermediate 56: step c) in THF (40 mL) was purged with N 2 for 10 minutes and cooled to −78° C. To the solution was added n-BuLi (1.6 M in hexanes, 2.1 mL, 3.4 mmol) dropwise. After stirring for 10 minutes at −78° C., a solution of (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (645 mg, 2.98 mmol, Intermediate 11: step b) in THF (5 mL) was added followed by 5 mL of THF all by cannula. The cooling bath was removed, and the reaction mixture was stirred at −78° C. to room temperature for 1 hour. Saturated NH 4 Cl (aqueous) was added and the organic layer was separated. The aqueous layer was extracted with dichloromethane. The combined organic phases were dried (Na 2 SO 4 ), filtered, and concentrated to afford an oil. Some solid precipitated after standing at room temperature for several hours. A small amount of DCM was added, the white solid was filtered, washed with Et 2 O, and dried under vacuum overnight to provide the title compound. The filtrate was concentrated and purified by flash column chromatography (silica gel, 30-100% EtOAc/heptane) to give more of the racemic product. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.06 (d, J=2.02 Hz, 1H), 7.81 (d, J=8.59 Hz, 1H), 7.36 (dd, J=2.02, 8.59 Hz, 1H), 6.94 (d, J=9.09 Hz, 3H), 4.10 (s, 3H), 3.94 (s, 3H), 2.89 (d, J=7.07 Hz, 2H), 2.55 (s, 3H), 2.38 (s, 3H), 2.02-2.13 (m, 2H), 1.78-1.90 (m, 1H), 1.68-1.77 (m, 3H), 1.57-1.68 (m, 1H), 1.46-1.60 (m, 2H); MS m/e 542.1 [M+H] + .

Example 1a was purified by chiral HPLC (Chiralcel OD, MeOH) to give 2 enantiomers. Example 1b: (first enantiomer to elute off chiral column) 1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 8.09 (d, J=2.02 Hz, 1H), 7.83 (d, J=8.59 Hz, 1H), 7.42 (dd, J=2.27, 8.84 Hz, 1H), 7.39 (s, 1H), 7.06 (d, J=8.08 Hz, 1H), 6.93 (d, J=8.08 Hz, 1H), 6.90 (s, 1H), 4.04 (s, 3H), 3.84 (s, 3H), 2.85 (d, J=7.07 Hz, 2H), 2.43 (s, 3H), 2.22 (s, 3H), 1.91-2.05 (m, 2H), 1.61-1.91 (m, 5H), 1.30-1.43 (m, 2H); MS m/e 542.3 [M+H] + and Example 1c: (second enantiomer to elute off chiral column, further purified by flash column chromatography (silica gel, 50-60% Et 2 O-DCM)). 1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 8.09 (s, 1H), 7.83 (d, J=8.59 Hz, 1H), 7.42 (d, J=9.09 Hz, 1H), 7.39 (s, 1H), 7.06 (d, J=8.08 Hz, 1H), 6.93 (d, J=8.08 Hz, 1H), 6.89 (s, 1H), 4.04 (s, 3H), 3.84 (s, 3H), 2.85 (d, J=7.07 Hz, 2H), 2.43 (s, 3H), 2.22 (s, 3H), 1.91-2.05 (m, 2H), 1.60-1.91 (m, 5H), 1.28-1.45 (m, 2H); MS m/e 542.3 [M+H] + .

Example 2a: 4-((4-Chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinolin-3-yl)methyl)tetrahydro-2H-thiopyran 1,1-dioxide

A mixture of (4-chloro-2-methoxy-3-((tetrahydro-2H-thiopyran-4-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol (726 mg, 1.39 mmol, Example 163), 3-chloroperbenzoic acid (˜77%, 1.24 g, 5.54 mmol) and DCM (70 mL) was stirred at room temperature for 1 hour. LCMS showed the major peak as MS m/e 572 [M+H] + .

›EXAMPLES · 26 of 31

To the mixture was added tribromophosphine (1.0 M in DCM, 5.0 mL, 5.0 mmol) dropwise, and a white suspension formed at the end of the addition. DMF (10 mL) was then added and the suspension dissolved. After stirring for 1 h, 1 M K 2 CO 3 (aqueous) was added, the organic layer was separated and the aqueous layer was extracted with DCM. The combined organic phases were washed with 1 M K 2 CO 3 (aqueous), and the aqueous layer was back extracted with DCM. The organic phases were combined, dried (Na 2 SO 4 ), filtered, and concentrated. The residue was purified by flash column chromatography (silica gel, 30-100% EtOAc in heptanes, 10% MeOH in DCM) to provide the title compound. MS m/e 556.0 [M+H] + .

Example 2a was purified by chiral HPLC (Chiralcel OD, MeOH) to give 2 enantiomers. The enantiomers were then further purified on plug silica gel columns (0-4% MeOH-DCM). Example 2b: (first enantiomer to elute off chiral column) 1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 8.10 (s, 1H), 7.83 (d, J=8.80 Hz, 1H), 7.37-7.48 (m, 2H), 7.06 (d, J=7.83 Hz, 1H), 6.92 (d, J=8.07 Hz, 1H), 6.89 (s, 1H), 4.05 (s, 3H), 3.84 (s, 3H), 3.04-3.15 (m, 2H), 2.94-3.04 (m, 2H), 2.87 (d, J=7.09 Hz, 2H), 2.43 (s, 3H), 2.22 (s, 3H), 1.99-2.14 (m, 1H), 1.86-1.98 (m, 2H), 1.72-1.86 (m, 2H); MS m/e 556.2 [M+H] + and Example 2c: (second enantiomer to elute off chiral column) 1 H NMR (400 MHz, DMSO-d 6 ) δ ppm 8.10 (s, 1H), 7.84 (d, J=8.56 Hz, 1H), 7.38-7.47 (m, 2H), 7.06 (d, J=8.07 Hz, 1H), 6.92 (d, J=8.07 Hz, 1H), 6.89 (s, 1H), 4.05 (s, 3H), 3.84 (s, 3H), 3.04-3.15 (m, 2H), 2.94-3.04 (m, 2H), 2.87 (d, J=7.09 Hz, 2H), 2.43 (s, 3H), 2.22 (s, 3H), 2.00-2.14 (m, 1H), 1.86-1.98 (m, 2H), 1.72-1.85 (m, 2H); MS m/e 556.2 [M+H] + .

Example 3: (4-Chlorophenyl)(2,4-dichloro-3-(morpholinomethyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)methanol.TFA

Into a 100-mL round-bottom flask purged and maintained with an inert atmosphere of nitrogen, was placed a solution of 6-bromo-2,4-dichloro-3-(morpholin-4-ylmethyl)quinoline (180 mg, 0.48 mmol, 100%, Intermediate 1: step e) in tetrahydrofuran (10 mL). Then n-BuLi (0.23 mL, 0.58 mmol, 2.5 M in hexanes) was added at −78° C. The resulting mixture was stirred for 10 minutes at −78° C., and then 5-[(4-chlorophenyl)carbonyl]-1-methyl-1H-imidazole (116 mg, 0.53 mmol, Intermediate 22: step b) was added to this solution. The resulting mixture was allowed to react, with stirring, for an additional 8 hours at room temperature. The reaction was then quenched by the addition of 10 mL of water. The resulting solution was extracted with 3×50 mL of ethyl acetate. The combined organic layer was dried over anhydrous sodium sulfate, filtered and concentrated under vacuum. The crude product was purified by Prep-HPLC with the following conditions (1#-Waters 2767-1): Column, SunFire Prep C18, 5 μm, 19×150 mm; mobile phase, water in 0.05% TFA and CH 3 CN (46% CH 3 CN up to 90% in 10 minutes, up to 100% in 2 minutes, down to 46% in 2 minutes) to afford the title compound trifluoroacetic acid salt as a white solid. 1 H NMR (300 MHz, CD 3 OD) δ ppm 9.50 (s, 1H), 8.86 (s, 1H), 8.60-8.57 (d, J=8.7 Hz, 1H), 8.48-8.44 (m, 1H), 7.98-7.91 (m, 4H), 7.46 (s, 1H), 5.37 (s, 3H), 4.64 (m, 4H), 4.40 (s, 2H), 4.19-3.98 (m, 4H); MS (ES, m/z) 517 [M+H] + .

Example 4a: 1-(4-((4-Chloro-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone

A solution of 4-chloro-6-iodo-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinoline (161.8 mg, 0.387 mmol, Intermediate 4: step c) in THF (1.9 mL) was stirred on an ice bath under argon while iPrMgCl (2.01 M in THF, 0.212 mL, 0.426 mmol) was added dropwise over 1 minute, and was stirred for another 7 minutes before transferring the reaction to a dry ice/CH 3 CN bath. In a separate vial, LaCl 3 -2LiCl (0.5 M in THF, 0.775 mL, 0.387 mmol) was added under argon to a solution of 1-(4-benzoylpiperidin-1-yl)ethanone (81.2 mg, 0.351 mmol, Intermediate 7) in THF (1.9 mL), and this was added dropwise over 1 minute to the ˜−50° C. Grignard solution which had been stirring in the dry ice/CH 3 CN bath for 8 minutes. After stirring for 1 additional minute in the dry ice/CH 3 CN bath, the reaction was transferred to an ice bath and stirred at 0° C. for 20 minutes, and was then quenched in one portion with 5 M aqueous NH 4 Cl (0.128 mL), dried (Na 2 SO 4 ), filtered through Celite®, and concentrated. The residue was dry load flash chromatographed (EtOAc isocratic elution) to afford the title compound contaminated with starting material 1-(4-benzoylpiperidin-1-yl)ethanone. This was further purified by C18 HPLC (20% to 100% CH 3 CN gradient with 0.1% TFA throughout). The product fractions were combined, neutralized with 2 M aqueous K 2 CO 3 , and concentrated to remove the organic solvent. The aqueous layer was then extracted with DCM, and the organic layer was dried (Na 2 SO 4 ), filtered, and concentrated to dryness to provide the title compound as a white foam. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.26 (d, J=9.09 Hz, 1H), 7.71-7.78 (m, 1H), 7.59-7.69 (m, 1H), 7.52 (t, J=6.82 Hz, 2H), 7.29-7.38 (m, 2H), 7.18-7.25 (m, 1H), 4.62-4.76 (m, 1H), 4.05 (s, 3H), 3.93 (d, J=11.12 Hz, 2H), 3.83 (t, J=15.41 Hz, 1H), 3.25-3.38 (m, 2H), 3.09 (qd, J=2.53, 13.31 Hz, 1H), 2.89 (d, J=7.07 Hz, 2H), 2.76 (t, J=11.87 Hz, 1H), 2.51-2.66 (m, 1H), 2.05 (s, ˜1.5H), 2.04 (s, ˜1.5H), 1.87-2.02 (m, 1H), 1.62-1.74 (m, 1H), 1.28-1.57 (m, 7H); MS m/e 523.2 [M+H] + .

Example 4a was purified by chiral HPLC (Chiralcel OD, 100% CH 3 CN) to give 2 enantiomers. Example 4b: (first enantiomer to elute off chiral column) 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.26 (d, J=7.58 Hz, 1H), 7.70-7.78 (m, 1H), 7.60-7.70 (m, 1H), 7.53 (t, J=6.82 Hz, 2H), 7.29-7.38 (m, 2H), 7.17-7.26 (m, 1H), 4.68 (t, J=13.39 Hz, 1H), 4.05 (s, 3H), 3.93 (d, J=11.12 Hz, 2H), 3.82 (t, J=14.15 Hz, 1H), 3.25-3.38 (m, 2H), 3.00-3.16 (m, 1H), 2.88 (d, J=7.07 Hz, 2H), 2.76 (t, J=11.62 Hz, 1H), 2.50-2.63 (m, 1H), 2.04 (s, ˜1.5H), 2.03 (s, ˜1.5H), 1.95 (dt, J=7.71, 14.91 Hz, 1H), 1.66 (t, J=15.66 Hz, 1H), 1.29-1.59 (m, 7H); MS m/e 523.2 [M+H] + ; and Example 4c: (second enantiomer to elute off chiral column) 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.27 (d, J=7.58 Hz, 1H), 7.70-7.78 (m, 1H), 7.60-7.70 (m, 1H), 7.53 (t, J=6.82 Hz, 2H), 7.29-7.37 (m, 2H), 7.17-7.26 (m, 1H), 4.67 (t, J=13.39 Hz, 1H), 4.05 (s, 3H), 3.93 (d, J=10.61 Hz, 2H), 3.82 (t, J=14.40 Hz, 1H), 3.25-3.38 (m, 2H), 3.01-3.16 (m, 1H), 2.88 (d, J=7.07 Hz, 2H), 2.70-2.81 (m, 1H), 2.50-2.65 (m, 1H), 2.04 (s, ˜1.5H), 2.04 (s, ˜1.5H), 1.86-1.99 (m, 1H), 1.56-1.73 (m, 1H), 1.29-1.55 (m, 7H); MS m/e 523.2 [M+H] + .

›EXAMPLES · 27 of 31

Example 5a: 1-(4-((4-Chloro-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinolin-6-yl)(hydroxy)(1-methyl-1H-imidazol-5-yl)methyl)piperidin-1-yl)ethanone

A solution of (4-chloro-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(piperidin-4-yl)methanol (232 mg, 0.479 mmol, Example 171) in TEA (0.0733 mL, 0.527 mmol) and DCM (9.6 mL) was stirred at ˜0° C. under argon while acetic anhydride was added dropwise over 1 minute. The reaction was stirred at 0° C. for 30 minutes and was then washed with 1 M aqueous NaH 2 PO 4 (1×8 mL), water (2×8 mL), and 2 M aqueous K 2 CO 3 (1×3 mL). The organic layer was dried (Na 2 SO 4 ), filtered, and concentrated to provide the title compound as a white foam. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.14 (s, ˜0.5H), 8.06 (s, ˜0.5H), 7.74 (dd, J=4.04, 8.59 Hz, 1H), 7.38 (t, J=9.85 Hz, 1H), 7.29 (s, 1H), 7.17 (s, 1H), 4.75 (d, J=13.64 Hz, ˜0.5H), 4.57 (d, J=13.1 Hz, ˜0.5H), 4.08 (s, 3H), 3.89-3.98 (m, ˜2.5H), 3.66-3.77 (m, ˜0.5H), 3.28-3.39 (m, 2H), 3.25 (s, ˜1.5H), 3.23 (s, ˜1.5H), 3.11-3.21 (m, ˜0.5H), 2.96 (m, ˜0.5H), 2.85-2.93 (m, 2H), 2.56-2.68 (m, ˜0.5H), 2.37-2.52 (m, ˜1.5H), 2.23-2.36 (m, 1H), 2.03 (s, ˜1.5H), 1.98 (s, ˜1.5H), 1.93-2.00 (m, 1H), 1.10-1.60 (m, 7H); MS m/e 527.2 [M+H] + .

Example 5a was purified by chiral HPLC (Chiralcel OD, 20% EtOH in heptane, then 100% EtOH) to give 2 enantiomers. Example 5b: (first enantiomer to elute off chiral column) 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.13 (s, ˜0.5H), 8.06 (s, ˜0.5H), 7.75 (dd, J=4.04, 9.09 Hz, 1H), 7.38 (dd, J=8.59, 14.15 Hz, 1H), 7.29 (d, J=4.04 Hz, 1H), 7.19 (s, 1H), 4.75 (d, J=13.1H, ˜0.5 Hz), 4.58 (d, J=13.2 Hz, ˜0.5H), 4.08 (s, 3H), 3.89-3.98 (m, ˜2.5H), 3.69-3.75 (m, ˜0.5H), 3.27-3.38 (m, 2H), 3.24 (s, ˜1.5H), 3.23 (s, ˜1.5H), 3.12-3.20 (m, ˜0.5H), 2.93-3.10 (m, ˜0.5H), 2.85-2.93 (m, 2H), 2.56-2.67 (m, ˜0.5H), 2.38-2.52 (m, ˜1.5H), 2.21-2.37 (m, 1H), 2.03 (s, ˜1.5H), 2.01 (s, ˜1.5H), 1.93-2.00 (m, 1H), 1.14-1.58 (m, 7H); MS m/e 527.2 [M+H] + ; and Example 5c: (second enantiomer to elute off chiral column) 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.13 (s, ˜0.5H), 8.07 (s, ˜0.5H), 7.75 (dd, J=4.04, 8.59 Hz, 1H), 7.33-7.44 (m, 1H), 7.28 (d, J=4.55 Hz, 1H), 7.18 (s, 1H), 4.76 (d, J=12.8H, 0.5H), 4.57 (d, J=13.0 Hz, ˜0.5H), 4.08 (s, 3H), 3.86-4.01 (m, ˜2.5H), 3.68-3.78 (m, ˜0.5H), 3.27-3.38 (m, 2H), 3.24 (s, ˜1.5H), 3.23 (s, ˜1.5H), 3.10-3.21 (m, ˜0.5H), 2.93-3.02 (m, ˜0.5H), 2.90 (d, J=7.07 Hz, 2H), 2.56-2.67 (m, ˜0.5H), 2.38-2.52 (m, ˜1.5H), 2.21-2.36 (m, 1H), 2.02 (s, ˜1.5H), 2.01 (s, ˜1.5H), 1.92-2.01 (m, 1H), 1.14-1.58 (m, 7H); MS m/e 527.2 [M+H] + .

Example 6a: (4-Chloro-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(1-(methylsulfonyl)piperidin-4-yl)methanol

A solution of (4-chloro-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(piperidin-4-yl)methanol (106 mg, 0.219 mmol, Example 171) in TEA (0.0334 mL, 0.24 mmol) and DCM (0.88 mL) was stirred at 0° C. under argon while CH 3 SO 2 Cl (0.0178 mL, 0.229 mmol) was added dropwise over ˜1 minute. The solution was stirred at ˜0° C. while allowing the ice bath to expire overnight. The reaction was concentrated and partitioned with 9:1 EtOAc/MeOH (8 mL) and water (8 mL), and the organic layer was washed with water (1×8 mL), 1 M aqueous NaH 2 PO 4 (1×8 mL), and 2 M aqueous K 2 CO 3 (1×3 mL), dried (Na 2 SO 4 ), filtered, and concentrated to provide the title compound as a white foam. The combined aqueous layers were made basic with 5 M aqueous K 2 CO 3 and extracted with 9:1 EtOAc/MeOH (3×30 mL). The combined organic layers were dried (Na 2 SO 4 ), filtered, and concentrated to provide additional title compound. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.08 (s, 1H), 7.75 (d, J=8.59 Hz, 1H), 7.37 (d, J=8.3 Hz, 1H), 7.34 (s, 1H), 7.19 (s, 1H), 4.08 (s, 3H), 3.93 (d, J=2.53 Hz, 3H), 3.71-3.79 (m, 1H), 3.28-3.38 (m, 2H), 3.24 (s, 3H), 2.90 (d, J=7.07 Hz, 2H), 2.73-2.82 (m, 4H), 2.54-2.63 (m, 1H), 2.29-2.42 (m, 2H), 1.91-2.04 (m, 1H), 1.19-1.60 (m, 7H); MS m/e 563.3 [M+H] + .

Example 6a was purified by chiral HPLC (Chiralcel OD, 50% EtOH in heptane) to give 2 enantiomers. Example 6b: (first enantiomer to elute off chiral column) 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.09 (s, 1H), 7.75 (d, J=8.59 Hz, 1H), 7.37 (d, J=9.09 Hz, 1H), 7.33 (s, 1H), 7.18 (s, 1H), 4.08 (s, 3H), 3.89-3.99 (m, 3H), 3.75 (d, J=11.12 Hz, 1H), 3.29-3.39 (m, 2H), 3.24 (s, 3H), 2.90 (d, J=7.07 Hz, 2H), 2.72-2.82 (m, 4H), 2.58 (td, J=3.03, 11.87 Hz, 1H), 2.28-2.43 (m, 2H), 1.92-2.04 (m, 1H), 1.20-1.60 (m, 7H); MS m/e 563.3 [M+H] + ; and Example 6c: (second enantiomer to elute off chiral column) 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.09 (s, 1H), 7.75 (d, J=9.09 Hz, 1H), 7.38 (d, J=7.07 Hz, 1H), 7.32 (s, 1H), 7.17 (s, 1H), 4.08 (s, 3H), 3.94 (d, J=11.12 Hz, 3H), 3.70-3.80 (m, 1H), 3.28-3.37 (m, 2H), 3.24 (s, 3H), 2.87-2.94 (m, 2H), 2.72-2.82 (m, 4H), 2.58 (td, J=3.03, 11.87 Hz, 1H), 2.28-2.42 (m, 2H), 1.91-2.05 (m, 1H), 1.17-1.59 (m, 7H); MS m/e 563.3 [M+H] + .

Example 7a: 1-(4-((4-Chloro-2-methoxy-3-(tetrahydro-2H-pyran-4-yl)quinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone

The title compound was prepared as described for Example 4a using 4-chloro-6-iodo-2-methoxy-3-(tetrahydro-2H-pyran-4-yl)quinoline (Intermediate 6: step d) in place of 4-chloro-6-iodo-2-methoxy-3-((tetrahydro-2H-pyran-4-yl)methyl)quinoline (Intermediate 4: step c), except 1.0 equivalent of each reagent was used. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.29 (dd, J=2.02, 8.59 Hz, 1H), 7.69-7.77 (m, 1H), 7.58-7.68 (m, 1H), 7.52 (t, J=6.82 Hz, 2H), 7.29-7.38 (m, 2H), 7.18-7.24 (m, 1H), 4.62-4.76 (m, 1H), 4.01-4.15 (m, 5H), 3.69-3.85 (m, 2H), 3.57 (t, J=11.12 Hz, 2H), 3.01-3.17 (m, 1H), 2.70-2.83 (m, 1H), 2.49-2.66 (m, 3H), 2.06 (s, ˜1.5H), 2.05 (s, ˜1.5H), 1.61-1.76 (m, 1H), 1.43-1.52 (m, 3H), 1.28-1.43 (m, 2H); MS m/e 509.1 [M+H] + .

Example 7a was purified by chiral HPLC (Chiralpak AS, 95% CH 3 CN, 5% EtOH) to give 2 enantiomers. Example 7b: (first enantiomer to elute off chiral column) 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.30 (d, J=7.07 Hz, 1H), 7.69-7.76 (m, 1H), 7.58-7.69 (m, 1H), 7.52 (t, J=7.07 Hz, 2H), 7.28-7.37 (m, 2H), 7.18-7.25 (m, 1H), 4.68 (t, J=14.40 Hz, 1H), 4.03-4.14 (m, 5H), 3.69-3.89 (m, 2H), 3.57 (t, J=11.12 Hz, 2H), 3.01-3.16 (m, 1H), 2.70-2.82 (m, 1H), 2.50-2.65 (m, 3H), 2.03 (s, 3H), 1.61-1.76 (m, 1H), 1.46 (d, J=11.62 Hz, 3H), 1.30-1.42 (m, 2H); MS m/e 509.2 [M+H] + ; and Example 7c: (second enantiomer to elute off chiral column) 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.29 (d, J=7.07 Hz, 1H), 7.70-7.75 (m, 1H), 7.60-7.67 (m, 1H), 7.52 (t, J=7.07 Hz, 2H), 7.30-7.36 (m, 2H), 7.18-7.25 (m, 1H), 4.69 (t, J=14.40 Hz, 1H), 4.05-4.12 (m, 5H), 3.65-3.88 (m, 2H), 3.57 (t, J=11.12 Hz, 2H), 3.03-3.15 (m, 1H), 2.76 (t, J=11.87 Hz, 1H), 2.52-2.64 (m, 3H), 2.05 (s, ˜1.5H), 2.05 (s, ˜1.5H), 1.64-1.73 (m, 1H), 1.53-1.64 (m, 3H), 1.23-1.50 (m, 2H); MS m/e 509.2 [M+H] + .

›EXAMPLES · 28 of 31

Example 8: Ethyl 6-(hydroxydi(pyridin-3-yl)methyl)-2,4-bis(trifluoromethyl)quinoline-3-carboxylate.TFA

An opaque yellow slurry of ethyl 6-bromo-2,4-bis(trifluoromethyl)quinoline-3-carboxylate (0.318 g, 0.764 mmol, Intermediate 2: step c) and di(pyridin-3-yl)methanone (0.155 g, 0.841 mmol) in THF (6 mL) at −70° C. (internal temperature) was stirred under argon while n-BuLi (0.577 mL, 1.59 M in hexane, 0.917 mmol) was added dropwise. The reaction was stirred at −70° C. for 30 minutes, and was then removed from the cold bath and allowed to warm to 0° C. over 11 minutes. The homogeneous dark brown solution was then quenched with 1 M HCl (aq) (1 mL) and partitioned with 1 M aqueous NaHCO 3 (3 mL). The aqueous layer was extracted with EtOAc (1×4 mL), and the combined organic layers were dried (Na 2 SO 4 ), filtered, and concentrated to dryness. The residue was flash chromatographed (0-100% EtOAc in heptane) and further purified by C18 HPLC (20-100% CH 3 CN in water with 0.1% TFA throughout) to provide, after lyophilization, the title compound as a pale yellow gum. 1 H NMR (400 MHz, CD 3 OD) δ ppm 8.83 (d, J=2.02 Hz, 2H), 8.76 (dd, J=1.01, 5.22 Hz, 2H), 8.40 (d, J=9.09 Hz, 1H), 8.34-8.37 (m, 1H), 8.27-8.31 (m, 2H), 8.07 (dd, J=2.02, 9.09 Hz, 1H), 7.84 (dd, J=5.56, 8.08 Hz, 2H), 4.48 (q, J=7.41 Hz, 2H), 1.40 (t, J=7.33 Hz, 3H); MS m/e 522.2 [M+H] + .

Example 9: 1-(4-(Hydroxy(1-methyl-1H-imidazol-5-yl)(3-(piperidine-1-carbonyl)-2,4-bis(trifluoromethyl)quinolin-6-yl)methyl)piperidin-1-yl)ethanone

A ˜1:1 mixture of (6-(hydroxy(1-methyl-1H-imidazol-5-yl)(piperidin-4-yl)methyl)-2,4-bis(trifluoromethyl)quinolin-3-yl)(piperidin-1-yl)methanone and (2-(1-methyl-1H-imidazol-5-yl)-3-(piperidine-1-carbonyl)-2,4-bis(trifluoromethyl)-1,2-dihydroquinolin-6-yl)(piperidin-4-yl)methanone (94.1 mg total, 0.165 mmol total, Example 165) in DCM (1 mL) and TEA (0.0299 mL, 0.215 mmol) was treated with acetic anhydride dropwise at room temperature, and was stirred for 45 minutes. The reaction was then concentrated, partitioned with EtOAc (3 mL) and 1 M aqueous NaHCO 3 (3 mL), and the aqueous layer was extracted with EtOAc (1×3 mL). The combined organic layers were dried (Na 2 SO 4 ), filtered, and concentrated to provide a ˜1:1 mixture of the title compound and 1-(4-(2-(1-methyl-1H-imidazol-5-yl)-3-(piperidine-1-carbonyl)-2,4-bis(trifluoromethyl)-1,2-dihydroquinoline-6-carbonyl)piperidin-1-yl)ethanone. This mixture was dissolved in MeOH (2.8 mL) and treated with NaBH 4 (19.6 mg, 0.519 mmol) with stirring for 7 minutes at room temperature to reduce the (2-(1-methyl-1H-imidazol-5-yl)-3-(piperidine-1-carbonyl)-2,4-bis(trifluoromethyl)-1,2-dihydroquinolin-6-yl)(piperidin-4-yl)methanone side product to allow for easier isolation of pure title compound via chromatography. The reaction was quenched with HOAc (0.2 mL) followed by 1 M aqueous NaHCO 3 (3 mL), and extracted with CHCl 3 (3×3 mL). The combined organic layers were dried (Na 2 SO 4 ), filtered, and concentrated. The residue was flash chromatographed (0-10% MeOH in DCM) and further purified with C18 HPLC (20% CH 3 CN to 100% CH 3 CN gradient, with 0.1% TFA throughout) to provide, after lyophilization, the TFA salt of the title compound. This was partitioned between DCM (3×2 mL) and 2 M aqueous K 2 CO 3 , and the combined organic layers were dried (Na 2 SO 4 ), filtered, and concentrated to provide the title compound (4 diastereomers with atropisomerism) as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.40-8.51 (m, “0.82H”), 8.28-8.18 (m, “1.18H”), 7.79-7.84 (m, “0.15H”), 7.62-7.73 (m, “0.59H”), 7.54-7.59 (m, “0.26H”), 7.41-7.47 (m, 1H), 7.20 (s, 1H), 4.51-4.71 (m, 1H), 3.81-3.97 (m, “1.44H”), 3.65-3.76 (m, “1.66H”), 3.35 (s, “0.70H”), 3.29 (s, “1.10H”), 3.27 (s, “0.49H”), 3.26 (s, “0.71H”), 3.12-3.23 (m, “2.42H”), 2.90-3.03 (m, “0.58H”), 2.58-2.68 (m, “0.63H”), 2.37-2.57 (m, “2.14H”), 2.28-2.37 (m, “0.63H”), 2.16-2.27 (m, “0.61H”), 2.03 (s, “0.71H”), 2.01 (s, “0.52H”), 1.96 (s, “0.74H”), 1.96 (s, “1.03H”), 1.70 (br. s, 4H), 1.08-1.48 (m, 4H); MS m/e 612.3 [M+H] + .

Example 10: (2,4-Dimethylthiazol-5-yl)(3-methoxy-2-phenylquinolin-6-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A yellow solution of 6-bromo-3-methoxy-2-phenylquinoline (61 mg, 0.194 mmol, Intermediate 8) in THF (2.2 mL) was stirred under argon ˜−70° C. while n-BuLi (1.60 M in hexane, 0.121 mL, 0.194 mmol) was added dropwise over 30 seconds. The resulting dark solution was stirred in the cold bath for another 1.5 minutes, and was then treated with a solution of (2,4-dimethylthiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (43.8 mg, 0.197 mmol, Intermediate 12: step b) in THF (0.8 mL) over 1 minute. After an additional 3 minutes, the reaction was removed from the cold bath and stirred under ambient conditions for 1 minute, and was then transferred to an ice bath and stirred at 0° C. for 45 minutes. The yellow homogeneous reaction was then quenched with 5 M aqueous NH 4 Cl (0.060 mL), dried (Na 2 SO 4 ), filtered, and concentrated. The residue was purified by FCC(EtOAc) to provide the title compound. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.16 (d, J=8.59 Hz, 1H), 7.96 (dd, J=1.66, 7.96 Hz, 2H), 7.54-7.61 (m, 2H), 7.46-7.54 (m, 3H), 7.44 (s, 1H), 7.30 (s, 1H), 3.97 (s, 3H), 3.95 (s, 3H), 3.29 (s, 1H), 2.61 (s, 3H), 2.18 (s, 3H); MS m/e 458.1 [M+H] + .

Example 11a: 1-(4-((4-Chloro-2-methoxy-3-methylquinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone

A solution of n-butyllithium in hexanes (1.6 M, 2.0 mL, 3.2 mmol) was added dropwise to a stirring solution of 6-bromo-4-chloro-2-methoxy-3-methylquinoline (1.0 g, 3.5 mmol, Intermediate 9: step b) in tetrahydrofuran (15 mL) at −78° C. After 5 minutes, a solution of 1-(4-benzoylpiperidin-1-yl)ethanone (814 mg, 3.5 mmol, Intermediate 7) in tetrahydrofuran (5 mL) was added dropwise. After 5 minutes, the flask was placed into an ice-water bath. After 90 minutes, water (10 mL) and ethyl acetate (100 mL) were added. The biphasic mixture was stirred at 23° C. for 5 minutes. Half-saturated aqueous sodium chloride solution was added and the layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Silica gel (5 g) was added to the filtrate and the mixture was concentrated by rotary evaporation to afford a free-flowing powder. The powder was loaded onto a silica gel column for flash-column chromatography purification. Elution with 100% hexanes initially, grading to 100% ethyl acetate provided the title compound as a white solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.27-8.21 (m, 1H), 7.77-7.70 (m, 1H), 7.66-7.58 (m, 1H), 7.55-7.48 (m, 2H), 7.37-7.28 (m, 2H), 7.26-7.18 (m, 1H), 4.74-4.63 (m, 1H), 4.06 (s, 3H), 3.87-3.76 (m, 1H), 3.15-3.01 (m, 1H), 2.79-2.70 (m, 1H), 2.64-2.51 (m, 1H), 2.42 (s, 3H), 2.29 (d, J=1.9 Hz, 1H), 2.05 (s, 3H), 1.72-1.57 (m, 1H), 1.56-1.28 (m, 3H); MS (ESI): mass calcd. for C 25 H 27 ClN 2 O 3 , 438.2; m/z found, 439.0 [M+H] + . 1-(4-((4-Chloro-2-methoxy-3-methylquinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 60% CO 2 , 40% mixture of methanol-isopropanol 50/50 v/v) to provide two enantiomers. The first eluting enantiomer was Example 11b: 1 H NMR (400 MHz, CDCl 3 , * denotes rotameric peaks) δ ppm 8.26-8.22 (m, 1H), 7.77-7.71 (m, 1H), 7.66-7.58 (m, 1H), 7.55-7.48 (m, 2H), 7.37-7.29 (m, 2H), 7.25-7.18 (m, 1H), 4.77-4.62 (m, 1H), 4.06 (s, 3H), 3.82 (t, J=14.1 Hz, 1H), 3.17-3.00 (m, 1H), 2.82-2.67 (m, 1H), 2.64-2.52 (m, 1H), 2.42 (s, 3H), 2.21 (s, 1H), 2.05* (s, 3H), 2.04* (s, 3H), 1.71-1.30 (m, 4H); MS (ESI): mass calcd. for C 25 H 27 ClN 2 O 3 , 438.2; m/z found, 439.0 [M+H] + and the second eluting enantiomer was Example 11c: 1 H NMR (400 MHz, CDCl 3 , * denotes rotameric peaks) δ 8.26-8.22 (m, 1H), 7.74 (dd, J=8.8, 6.1 Hz, 1H), 7.66-7.59 (m, 1H), 7.55-7.48 (m, 2H), 7.37-7.30 (m, 2H), 7.25-7.18 (m, 1H), 4.75-4.62 (m, 1H), 4.06 (s, 3H), 3.89-3.77 (m, 1H), 3.16-3.01 (m, 1H), 2.81-2.69 (m, 1H), 2.64-2.51 (m, 1H), 2.42 (s, 3H), 2.20 (s, 1H), 2.05* (s, 3H), 2.04* (s, 3H), 1.72-1.29 (m, 4H); MS (ESI): mass calcd. for C 25 H 27 ClN 2 O 3 , 438.2; m/z found, 439.0 [M+H] + .

›EXAMPLES · 29 of 31

Example 12a: (4-Chloro-2-methoxy-3-methylquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A solution of n-butyllithium in hexanes (2.5 M, 0.28 mL, 0.70 mmol) was added dropwise to a stirring solution of 6-bromo-4-chloro-2-methoxy-3-methylquinoline (200 mg, 0.70 mmol, Intermediate 9: step b) in tetrahydrofuran (6 mL) at −78° C. After 2 minutes, a solution of (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (166 mg, 0.768 mmol, Intermediate 11: step b) in tetrahydrofuran (2 mL) was added dropwise. After 3 minutes, the flask was removed from the cooling bath and allowed to warm to 23° C. After 20 minutes, water (5 mL) and ethyl acetate (50 mL) were added. The biphasic mixture was poured into saturated aqueous sodium chloride solution (30 mL). The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Silica gel (3 g) was added to the filtrate and the mixture was concentrated by rotary evaporation to afford a free-flowing powder. The powder was loaded onto a silica gel column for flash column chromatography purification. Elution with dichloromethane initially, grading to 5% methanol-dichloromethane provided the title compound as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.04 (d, J=2.2 Hz, 1H), 7.80 (d, J=8.8 Hz, 1H), 7.36-7.28 (m, 1H), 7.00-6.90 (m, 3H), 4.11 (s, 3H), 3.94 (s, 3H), 3.62 (s, 1H), 2.55 (s, 3H), 2.43 (s, 3H), 2.38 (s, 3H); MS (ESI): mass calcd. for C 22 H 22 ClN 5 O 2 , 423.1; m/z found, 423.9 [M+H] + . (4-Chloro-2-methoxy-3-methylquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 80% CO 2 , 20% methanol containing 0.03% isopropylamine) to provide two enantiomers. The first eluting enantiomer was Example 12b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.04 (d, J=2.2 Hz, 1H), 7.80 (d, J=8.7 Hz, 1H), 7.31 (dd, J=8.7, 2.2 Hz, 1H), 6.99-6.92 (m, 3H), 4.11 (s, 3H), 3.94 (s, 3H), 3.61 (br s, 1H), 2.55 (s, 3H), 2.43 (s, 3H), 2.38 (s, 3H); MS (ESI): mass calcd. for C 22 H 22 ClN 5 O 2 , 423.1; m/z found, 424.4 [M+H] + and the second eluting enantiomer was Example 12c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.03 (d, J=2.2 Hz, 1H), 7.81 (d, J=8.7 Hz, 1H), 7.35-7.30 (m, 1H), 6.99-6.92 (m, 3H), 4.11 (s, 3H), 3.94 (s, 3H), 3.37 (br s, 1H), 2.56 (s, 3H), 2.44 (s, 3H), 2.39 (s, 3H); MS (ESI): mass calcd. for C 22 H 22 ClN 5 O 2 , 423.1; m/z found, 424.4 [M+H] + .

Example 13a: (4-Chloro-2-methoxy-3-methylquinolin-6-yl)(2,4-dimethylthiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A solution of n-butyllithium in hexanes (2.5 M, 0.47 mL, 1.2 mmol) was added dropwise to a stirring solution of 6-bromo-4-chloro-2-methoxy-3-methylquinoline (300 mg, 1.0 mmol, Intermediate 9: step b) in tetrahydrofuran (8 mL) at −78° C. After 2 minutes, a solution of (2,4-dimethylthiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (233 mg, 1.05 mmol, Intermediate 12: step b) in tetrahydrofuran (1.5 mL) was added dropwise. After 5 minutes, the flask was placed into an ice-water bath. After 60 minutes, water (5 mL) was added and the mixture was allowed to warm to 23° C. The mixture was partitioned between half-saturated aqueous sodium chloride solution (25 mL) and ethyl acetate (50 mL). The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Silica gel (3 g) was added to the filtrate and the mixture was concentrated by rotary evaporation to afford a free-flowing powder. The powder was loaded onto a silica gel column for flash column chromatography purification. Elution with 40% ethyl acetate-hexanes initially, grading to 90% ethyl acetate-hexanes provided the title compound as a white solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.12-8.07 (m, 1H), 7.86-7.81 (m, 1H), 7.51-7.45 (m, 1H), 7.25-7.22 (m, 1H), 4.11 (s, 3H), 3.92 (s, 3H), 3.50 (s, 1H), 2.59 (s, 3H), 2.44 (s, 3H), 2.15 (s, 3H); MS (ESI): mass calcd. for C 20 H 20 ClN 5 O2 S , 429.1; m/z found, 430.1 [M+H] + .

(4-Chloro-2-methoxy-3-methylquinolin-6-yl)(2,4-dimethylthiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD, 5 m, 250×30 mm, mobile phase: 75% CO 2 , 25% mixture containing methanol-isopropanol 50/50 v/v containing 0.03% isopropylamine) to provide two enantiomers. The first eluting enantiomer was Example 13b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.10 (d, J=2.3 Hz, 1H), 7.83 (d, J=8.7 Hz, 1H), 7.51-7.44 (m, 1H), 7.22 (s, 1H), 4.11 (s, 3H), 3.93 (s, 3H), 3.71 (s, 1H), 2.59 (s, 3H), 2.43 (s, 3H), 2.15 (s, 3H); MS (ESI): mass calcd. for C 20 H 20 ClN 5 O2 S , 429.1; m/z found, 430.0 [M+H] + and the second eluting enantiomer was Example 13c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.10 (d, J=2.2 Hz, 1H), 7.84 (d, J=8.7 Hz, 1H), 7.48 (dd, J=8.8, 2.2 Hz, 1H), 7.24 (s, 1H), 4.11 (s, 3H), 3.92 (s, 3H), 3.47 (s, 1H), 2.59 (s, 3H), 2.44 (s, 3H), 2.16 (s, 3H); MS (ESI): mass calcd. for C 20 H 20 ClN 5 O2 S , 429.1; m/z found, 430.0 [M+H] + .

Example 14a: (4-Chloro-3-(cyclopropylmethyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A solution of n-butyllithium (2.5 M in hexanes, 0.32 mL, 0.80 mmol) was added dropwise by syringe to a stirring solution of 4-chloro-3-(cyclopropylmethyl)-6-iodo-2-methoxyquinoline (300 mg, 0.80 mmol, Intermediate 13: step c) in dry THF (6 mL) at −78° C. After 1 minute, a solution of (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (174 mg, 0.803 mmol, Intermediate 11: step b) in dry THF (2 mL) was added dropwise by syringe. After 2 minutes, the flask was removed from the cooling bath and allowed to warm. After 2 minutes, the flask was placed into an ice-water bath. After 10 minutes, water (5 mL) was added. The biphasic mixture was partitioned between half-saturated aqueous sodium chloride solution (25 mL) and ethyl acetate (50 mL). The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Celite® (5 g) was added to the filtrate and the solvents were removed by rotary evaporation to provide a free-flowing powder. The powder was loaded onto a silica gel column. Elution with dichloromethane initially, grading to 5% methanol-dichloromethane provided the title compound as a white solid. 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.06 (d, J=2.2 Hz, 1H), 7.81 (d, J=8.7 Hz, 1H), 7.35-7.32 (m, 1H), 6.98-6.92 (m, 3H), 4.11 (s, 3H), 3.94 (s, 3H), 3.67 (s, 1H), 2.86 (d, J=6.9 Hz, 2H), 2.55 (s, 3H), 2.39 (s, 3H), 1.15-1.07 (m, 1H), 0.46-0.39 (m, 2H), 0.37-0.32 (m, 2H); MS (ESI): mass calcd. for C 25 H 26 ClN 5 O 2 , 463.2; m/z found, 464.1 [M+H] + . (4-Chloro-3-(cyclopropylmethyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 80% CO 2 , 20% mixture containing methanol-isopropanol 50/50 v/v containing 0.03% isopropylamine) to provide two enantiomers. The first eluting enantiomer was Example 14b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.05 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.36-7.32 (m, 1H), 6.99 (s, 1H), 6.99-6.93 (m, 2H), 4.11 (s, 3H), 3.95 (s, 3H), 3.28 (s, 1H), 2.87 (d, J=6.9 Hz, 2H), 2.56 (s, 3H), 2.40 (s, 3H), 1.17-1.06 (m, 1H), 0.47-0.40 (m, 2H), 0.38-0.31 (m, 2H); MS (ESI): mass calcd. for C 25 H 26 ClN 5 O 2 , 463.2; m/z found, 464.1 [M+H] + and the second eluting enantiomer was Example 14c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.06-8.04 (m, 1H), 7.84-7.81 (m, 1H), 7.34 (dd, J=8.7, 2.2 Hz, 1H), 6.99 (s, 1H), 6.98-6.93 (m, 2H), 4.11 (s, 3H), 3.94 (s, 3H), 3.31 (s, 1H), 2.87 (d, J=6.9 Hz, 2H), 2.56 (s, 3H), 2.40 (s, 3H), 1.17-1.06 (m, 1H), 0.47-0.41 (m, 2H), 0.37-0.31 (m, 2H); MS (ESI): mass calcd. for C 25 H 26 ClN 5 O 2 , 463.2; m/z found, 464.1 [M+H] + .

›EXAMPLES · 30 of 31

Example 15a: 1-(4-((4-Chloro-3-(cyclopropylmethyl)-2-methoxyquinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone

A solution of n-butyllithium (2.5 M in hexanes, 0.21 mL, 0.54 mmol) was added dropwise by syringe to a stirring solution of 4-chloro-3-(cyclopropylmethyl)-6-iodo-2-methoxyquinoline (200 mg, 0.54 mmol, Intermediate 13: step c) in dry tetrahydrofuran (4 mL) at −78° C. After 1 minute, a solution of 1-(4-benzoylpiperidin-1-yl)ethanone (124 mg, 0.54 mmol, Intermediate 7) in dry tetrahydrofuran (1 mL) was added dropwise by syringe. After 2 minutes, the flask was removed from the cooling bath and allowed to warm. After 2 minutes, the flask was placed into an ice-water bath. After 10 minutes, water (5 mL) and ethyl acetate (50 mL) were added. The biphasic mixture was poured into half-saturated aqueous sodium chloride solution (25 mL). The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Celite® (5 g) was added to the filtrate and the solvents were removed by rotary evaporation to provide a free-flowing powder. The powder was loaded onto a silica gel column. Elution with hexanes initially, grading to 90% ethyl acetate-hexanes provided the title compound as a white solid. MS (ESI): mass calcd. for C 28 H 31 ClN 2 O 3 , 478.2; m/z found, 479.1 [M+H] + .

1-(4-((4-Chloro-3-(cyclopropylmethyl)-2-methoxyquinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 65% CO 2 , 35% mixture containing methanol-isopropanol 50/50 v/v containing 0.03% isopropylamine) to provide two enantiomers. The first eluting enantiomer was Example 15b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.29-8.24 (m, 1H), 7.78-7.72 (m, 1H), 7.68-7.59 (m, 1H), 7.56-7.49 (m, 2H), 7.38-7.30 (m, 2H), 7.25-7.18 (m, 1H), 4.77-4.64 (m, 1H), 4.06 (s, 3H), 3.90-3.76 (m, 1H), 3.17-3.01 (m, 1H), 2.86 (d, J=6.8 Hz, 2H), 2.83-2.71 (m, 1H), 2.66-2.51 (m, 1H), 2.21 (s, 1H), 2.05 (s, 3H), 1.74-1.23 (m, 4H), 1.18-1.04 (m, 1H), 0.48-0.28 (m, 4H); MS (ESI): mass calcd. for C 28 H 31 ClN 2 O 3 , 478.2; m/z found, 479.1 [M+H] + and the second eluting enantiomer was Example 15c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.26 (dd, J=6.4, 2.1 Hz, 1H), 7.78-7.72 (m, 1H), 7.67-7.60 (m, 1H), 7.56-7.49 (m, 2H), 7.37-7.30 (m, 2H), 7.25-7.19 (m, 1H), 4.76-4.64 (m, 1H), 4.06 (s, 3H), 3.89-3.76 (m, 1H), 3.15-3.02 (m, 1H), 2.86 (d, J=6.8 Hz, 2H), 2.82-2.72 (m, 1H), 2.65-2.52 (m, 1H), 2.23 (s, 1H), 2.05 (s, 3H), 1.74-1.29 (m, 4H), 1.17-1.05 (m, 1H), 0.45-0.29 (m, 4H); MS (ESI): mass calcd. for C 28 H 31 ClN 2 O 3 , 478.2; m/z found, 479.1 [M+H] + .

Example 16: 1-((4-Chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methyl)-2-methoxyquinolin-3-yl)methyl)-4-(trifluoromethyl)piperidin-4-ol

A solution of methyllithium in ether (1.6 M, 0.300 mL, 0.480 mmol) was added dropwise to a dry ice-acetone cooled, stirring solution of 1-((6-bromo-4-chloro-2-methoxyquinolin-3-yl)methyl)-4-(trifluoromethyl)piperidin-4-ol (200 mg, 0.441 mmol, Intermediate 16) in dry tetrahydrofuran (4 mL). After 1 minute, a solution of n-butyllithium in hexanes (2.5 M, 0.180 mL, 0.450 mmol) was added dropwise by syringe. After 1 minute, a solution of (1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone (148 mg, 0.580 mmol, Intermediate 14: step b) in dry tetrahydrofuran (1 mL) was added dropwise by syringe. After 5 minutes, the flask was removed from the cooling bath. After 5 minutes, the flask was placed into an ice-water bath. After 15 minutes, water (20 mL) and ethyl acetate (50 mL) were added sequentially. The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Celite® (7 g) was added to the filtrate and the mixture was concentrated in vacuo. The dry solid was loaded onto a silica gel column for flash column chromatography. Elution with dichloromethane initially, grading to 10% methanol-dichloromethane provided the title compound as an off-white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.68 (d, J=5.1 Hz, 1H), 8.15 (d, J=2.1 Hz, 1H), 7.88 (d, J=1.6 Hz, 1H), 7.82 (d, J=8.8 Hz, 1H), 7.57-7.52 (m, 1H), 7.51-7.47 (m, 1H), 7.36 (s, 1H), 6.37 (s, 1H), 4.70 (s, 1H), 4.09 (s, 3H), 3.85 (s, 2H), 3.36 (s, 3H), 2.88-2.79 (m, 2H), 2.60-2.50 (m, 2H), 1.97 (s, 1H), 1.93-1.82 (m, 2H), 1.65 (d, J=13.3 Hz, 2H); MS (ESI): mass calcd. for C 28 H 26 ClF 6 N 5 O 3 , 629.2; m/z found, 630.0 [M+H] + .

Example 17a: (4-Chloro-2-methoxy-3-((4-(2,2,2-trifluoroethyl)piperazin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A 2.5 M solution of n-butyllithium in hexanes (0.260 mL, 0.650 mmol) was added dropwise to a dry ice-acetone cooled solution of 5-bromo-1,2-dimethyl-1H-imidazole (120 mg, 0.686 mmol) in tetrahydrofuran (2 mL). After 30 seconds, a solution of (4-chloro-2-methoxy-3-((4-(2,2,2-trifluoroethyl)piperazin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (157 mg, 0.325 mmol, Intermediate 17: step b) in tetrahydrofuran (1 mL) was added dropwise by syringe. After 2 minutes, the flask was removed from the cooling bath. After 3 minutes, the flask was placed into an ice-water bath. After 45 minutes, water (20 mL) and ethyl acetate (50 mL) were added sequentially. The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Celite® (7 g) was added to the filtrate and the mixture was concentrated in vacuo. The dry solid was loaded onto a silica gel column for flash-column chromatography. Elution with dichloromethane initially, grading to 10% methanol-dichloromethane provided impure title compound. Further purification by RP-HPLC eluting with 5% acetonitrile-water containing 0.2% TFA and partitioning of the combined fractions between dichloromethane-saturated aqueous sodium bicarbonate solution provided the title compound as a white solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.20 (d, J=2.2 Hz, 1H), 7.72 (d, J=8.7 Hz, 1H), 7.41-7.34 (m, 1H), 7.12 (s, 1H), 6.05 (s, 1H), 5.89 (s, 1H), 4.09 (s, 3H), 3.91 (s, 3H), 3.88-3.80 (m, 2H), 3.37 (s, 3H), 2.99-2.87 (m, 2H), 2.65 (s, 8H), 2.24 (s, 3H); MS (ESI): mass calcd. for C 26 H 30 ClF 3 N 8 O 2 , 578.2; m/z found, 579.1 [M+H] + . (4-Chloro-2-methoxy-3-((4-(2,2,2-trifluoroethyl)piperazin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250 mm×30 mm, mobile phase: 85% CO 2 , 15% isopropanol containing 0.2% isopropylamine) to provide two enantiomers. The first eluting enantiomer was Example 17b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.17 (d, J=2.1 Hz, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.40-7.35 (m, 1H), 7.18 (s, 1H), 6.13 (s, 1H), 4.54 (s, 1H), 4.09 (s, 3H), 3.92 (s, 3H), 3.85 (s, 2H), 3.40 (s, 3H), 2.98-2.89 (m, 2H), 2.70-2.62 (m, 8H), 2.33 (s, 3H); MS (ESI): mass calcd. for C 26 H 30 ClF 3 N 8 O 2 , 578.2; m/z found, 579.1 [M+H] + and the second eluting enantiomer was Example 17c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.16 (d, J=2.2 Hz, 1H), 7.77 (d, J=8.7 Hz, 1H), 7.41-7.36 (m, 1H), 7.20 (s, 1H), 6.16 (s, 1H), 4.16-4.06 (m, 4H), 3.93 (s, 3H), 3.86 (s, 2H), 3.41 (s, 3H), 2.98-2.88 (m, 2H), 2.71-2.61 (m, 8H), 2.35 (s, 3H); MS (ESI): mass calcd. for C 26 H 30 ClF 3 N 8 O 2 , 578.2; m/z found, 579.1 [M+H] + .

›EXAMPLES · 31 of 31

Example 18: (4-Chloro-2-methoxy-3-methylquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanol

A solution of n-butyllithium in hexanes (1.6 M, 0.65 mL, 1.0 mmol) was added dropwise to a stirring suspension of 6-bromo-4-chloro-2-methoxy-3-methylquinoline (0.3 g, 1.0 mmol, Intermediate 9: step b) in tetrahydrofuran (5 mL) at −78° C. After 3 minutes, a solution of (1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone (294 mg, 1.15 mmol, Intermediate 10: step c) in tetrahydrofuran (5 mL) was added dropwise. After 10 minutes, the flask was placed in an ice-water bath. After 20 minutes, water (5 mL) and ethyl acetate (25 mL) were added sequentially. The biphasic mixture was partitioned between saturated aqueous sodium chloride solution (50 mL) and ethyl acetate (50 mL). The layers were separated. The organic layer was dried with sodium sulfate and the dried solution was filtered. Silica gel (5 g) was added to the filtrate and the mixture was concentrated by rotary evaporation to afford a free-flowing powder. The powder was loaded onto a silica gel column for flash column chromatography purification. Elution with dichloromethane initially, grading to 5% methanol-dichloromethane provided the title compound as an off-white solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.81 (d, J=2.2 Hz, 1H), 8.10 (d, J=2.2 Hz, 1H), 7.89 (d, J=10.1 Hz, 1H), 7.80 (dd, J=8.6, 0.6 Hz, 1H), 7.65 (dd, J=8.2, 0.8 Hz, 1H), 7.48 (dd, J=8.8, 2.2 Hz, 1H), 7.37-7.32 (m, 1H), 6.36 (d, J=1.1 Hz, 1H), 4.70 (br s, 1H), 4.09 (s, 3H), 3.37 (s, 3H), 2.43 (s, 3H); MS (ESI): mass calcd. for C 22 H 18 ClF 3 N 4 O 2 , 462.1; m/z found, 463.0 [M+H] + .

›Examples42

›Example 19: (3-Chlorophenyl) [2,4-dichloro-3-(1-methylethyl)quinolin-6-yl]pyridin-3-ylmethanol.TFA

To a solution of 6-bromo-2,4-dichloro-3-isopropylquinoline (640 mg, 2.01 mmol, Intermediate 67: step a) and (3-chlorophenyl)(pyridin-3-yl)methanone (481 mg, 2.21 mmol) in THF (20 mL) at −78° C. was added n-BuLi (1.6 M in hexanes, 1.63 mL, 2.61 mmol). The resulting solution was stirred at −78° C. for 10 minutes, then allowed to warm to room temperature. The mixture was quenched with saturated aqueous NH 4 Cl and extracted with DCM. The organics were combined, dried, filtered and concentrated to dryness. The residue was purified by FCC (0-80% EtOAc/heptane) followed by HPLC to provide the title compound as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 1.50 (d, J=7.6 Hz, 6H), 3.93-4.16 (m, 1H), 7.11 (d, J=7.6 Hz, 1H), 7.23-7.30 (m, 1H), 7.30-7.40 (m, 2H), 7.59 (dd, J=8.6, 2.0 Hz, 1H), 7.85 (dd, J=8.6, 5.6 Hz, 1H), 7.98 (d, J=8.6 Hz, 1H), 8.14 (d, J=2.0 Hz, 1H), 8.27 (d, J=8.1 Hz, 1H), 8.81 (d, J=4.5 Hz, 1H), 9.11 (s, 1H). MS (ESI): mass calcd. for C 24 H 19 Cl 3 N 2 O, 456.1; m/z found, 456.8 [M+H] + .

Example 20: [4-Chloro-3-(1-methylethyl)-2-pyrimidin-5-ylquinolin-6-yl](3-chlorophenyl)pyridin-3-ylmethanol

A mixture of (3-chlorophenyl)[2,4-dichloro-3-(1-methylethyl)quinolin-6-yl]pyridin-3-ylmethanol (80 mg, 0.15 mmol, Example 19), pyrimidin-5-ylboronic acid (23 mg, 0.18 mmol), PdCl 2 (dppf) (11 mg, 0.015 mmol) and K 2 CO 3 (43 mg, 0.31 mmol) in 1,4-dioxane (10 mL) and water (2 mL) was heated to 70° C. for 3 hours. The mixture was cooled to room temperature, diluted with EtOAc and washed with water. The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness. The residue was purified by FCC (0-6% MeOH/EtOAc) to provide the title compound as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 1.45 (d, J=7.1 Hz, 6H), 3.40 (dt, J=14.3, 7.3 Hz, 1H), 3.72 (s, 1H), 7.19 (d, J=7.6 Hz, 1H), 7.28-7.39 (m, 4H), 7.64-7.74 (m, 2H), 8.04 (d, J=9.1 Hz, 1H), 8.32 (d, J=2.0 Hz, 1H), 8.57 (br. s., 2H), 8.87 (s, 2H), 9.32 (s, 1H). MS (ESI): mass calcd. for C 28 H 22 Cl 2 N 4 O, 500.1; m/z found, 500.9 [M+H] + .

›Example 21: (3-Chlorophenyl)(2,4-dichloro-3-methylquinolin-6-yl)pyridin-3-ylmethanol.TFA

The title compound was prepared using 6-bromo-2,4-dichloro-3-methylquinoline (Intermediate 9: step a) in place of 6-bromo-2,4-dichloro-3-isopropylquinoline using the procedure described for Example 19. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 2.65 (s, 3H), 7.11 (d, J=7.6 Hz, 1H), 7.27 (br. s., 1H), 7.29-7.38 (m, 2H), 7.57 (dd, J=8.6, 2.0 Hz, 1H), 7.82 (dd, J=8.1, 5.6 Hz, 1H), 7.96 (d, J=9.1 Hz, 1H), 8.06 (d, J=2.0 Hz, 1H), 8.23 (d, J=8.1 Hz, 1H), 8.76 (d, J=5.1 Hz, 1H), 9.09 (br. s., 1H). MS (ESI): mass calcd. for C 22 H 15 Cl 3 N 2 O, 428.0; m/z found, 428.9 [M+H] + .

›Example 22: (4-Chloro-3-methyl-2-pyrimidin-5-ylquinolin-6-yl)(3-chlorophenyl)pyridin-3-ylmethanol

A mixture of (3-chlorophenyl)(2,4-dichloro-3-methylquinolin-6-yl)pyridin-3-ylmethanol (80 mg, 0.16 mmol, Example 21), pyrimidin-5-ylboronic acid (24 mg, 0.19 mmol), PdCl 2 (dppf) (12 mg, 0.016 mmol) and K 2 CO 3 (44 mg, 0.32 mmol) in 1,4-dioxane (10 mL) and water (2 mL) was heated to 70° C. for 3 hours. The mixture was cooled to room temperature, diluted with EtOAc and washed with water. The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness. The residue was purified by FCC (0-6% MeOH/EtOAc) to provide the title compound as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 2.58 (s, 3H), 3.49 (s, 1H), 7.19 (d, J=7.1 Hz, 1H), 7.28-7.40 (m, 4H), 7.64-7.75 (m, 2H), 8.08 (d, J=8.6 Hz, 1H), 8.24 (d, J=2.0 Hz, 1H), 8.59 (br. s., 2H), 8.99 (s, 2H), 9.32 (s, 1H). MS (ESI): mass calcd. for C 26 H 18 Cl 2 N 4 O, 472.1; m/z found, 472.8 [M+H] + .

Example 23: [4-Chloro-3-(1-methylethyl)-2-thiophen-3-ylquinolin-6-yl](3-chlorophenyl)pyridin-3-ylmethanol

The title compound was prepared using thiophen-3-ylboronic acid in place of pyrimidin-5-ylboronic acid using the procedure described for Example 20. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 1.45 (d, J=7.1 Hz, 6H), 3.64 (quin, J=7.2 Hz, 1H), 7.10 (d, J=7.6 Hz, 1H), 7.28 (d, J=4.0 Hz, 2H), 7.31-7.40 (m, 3H), 7.53 (dd, J=5.1, 3.0 Hz, 1H), 7.69-7.77 (m, 2H), 7.80 (dd, J=8.1, 5.6 Hz, 1H), 8.23 (d, J=8.1 Hz, 1H), 8.27 (d, J=9.1 Hz, 1H), 8.32 (d, J=2.0 Hz, 1H), 8.76 (d, J=4.0 Hz, 1H), 8.95 (s, 1H). MS (ESI): mass calcd. for C 28 H 22 Cl 2 N 2 OS, 504.1; m/z found, 504.9 [M+H] + .

Example 24: [4-Chloro-3-methyl-2-(1-methyl-1H-indazol-5-yl)quinolin-6-yl](3-chlorophenyl)pyridin-3-ylmethanol

The title compound was prepared using (1-methyl-1H-indazol-5-yl)boronic acid in place of pyrimidin-5-ylboronic acid using the procedure described for Example 22. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 2.54 (s, 3H), 3.56 (s, 1H), 4.14 (s, 3H), 7.19 (d, J=7.3 Hz, 1H), 7.27-7.34 (m, 3H), 7.39 (s, 1H), 7.51 (d, J=8.6 Hz, 1H), 7.60 (d, J=8.8 Hz, 2H), 7.70 (d, J=8.1 Hz, 1H), 7.92 (s, 1H), 8.04-8.10 (m, 2H), 8.19 (d, J=1.7 Hz, 1H), 8.55 (d, J=3.9 Hz, 1H), 8.59 (br. s., 1H). MS (ESI): mass calcd. for C 30 H 22 Cl 2 N 4 O, 524.1; m/z found, 524.8 [M+H] + .

Example 25: [4-Chloro-3-methyl-2-(1-methyl-1H-indazol-6-yl)quinolin-6-yl](3-chlorophenyl)pyridin-3-ylmethanol

The title compound was prepared using (1-methyl-1H-indazol-6-yl)boronic acid in place of pyrimidin-5-ylboronic acid using the procedure described for Example 22. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 2.53 (s, 3H), 3.55 (s, 1H), 4.12 (s, 3H), 7.20 (d, J=7.1 Hz, 1H), 7.27-7.36 (m, 4H), 7.39 (s, 1H), 7.59-7.64 (m, 2H), 7.70 (d, J=8.1 Hz, 1H), 7.83 (d, J=7.6 Hz, 1H), 8.04 (s, 1H), 8.10 (d, J=8.6 Hz, 1H), 8.22 (d, J=2.0 Hz, 1H), 8.58 (d, J=9.1 Hz, 2H). MS (ESI): mass calcd. for C 30 H 22 Cl 2 N 4 O, 524.1; m/z found, 524.8 [M+H] + .

›Example 26: (3-tert-Butyl-2,4-dichloroquinolin-6-yl)(3-chlorophenyl)pyridin-3-ylmethanol.TFA

To a solution of 6-bromo-3-(tert-butyl)-2,4-dichloroquinoline (666 mg, 2 mmol, Intermediate 18: step b) and (3-chlorophenyl)(pyridin-3-yl)methanone (479 mg, 2.2 mmol) in THF (20 mL) at −78° C. was added n-BuLi (1.6 M in hexanes, 1.63 mL, 2.6 mmol). The resulting solution was stirred at −78° C. for 10 minutes, then allowed to warm to room temperature. The mixture was quenched with saturated aqueous NH 4 Cl, the layers separated, and the aqueous further extracted with DCM. The organics were combined, dried, filtered and concentrated to dryness. The residue was purified by FCC (0-80% EtOAc/heptane) followed by HPLC to provide the title compound as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 1.78 (s, 9H), 7.11 (d, J=7.6 Hz, 1H), 7.28 (s, 1H), 7.32-7.41 (m, 2H), 7.57 (dd, J=8.8, 2.3 Hz, 1H), 7.84 (dd, J=8.1, 5.6 Hz, 1H), 7.95 (d, J=8.6 Hz, 1H), 8.23 (d, J=2.0 Hz, 1H), 8.27 (d, J=8.1 Hz, 1H), 8.82 (d, J=4.5 Hz, 1H), 9.07 (s, 1H). MS (ESI): mass calcd. for C 25 H 21 Cl 3 N 2 O, 470.1; m/z found, 471.8 [M+H] + .

Example 27: {3-tert-Butyl-4-chloro-2-[(E)-2-phenylethenyl]quinolin-6-yl}(3-chlorophenyl)pyridin-3-ylmethanol.TFA

A mixture of (3-tert-butyl-2,4-dichloroquinolin-6-yl)(3-chlorophenyl)pyridin-3-ylmethanol (80 mg, 0.14 mmol, Example 26), (E)-styrylboronic acid (25 mg, 0.17 mmol), PdCl 2 (dppf) (11 mg, 0.014 mmol) and K 2 CO 3 (39 mg, 0.28 mmol) in 1,4-dioxane (10 mL) and water (2 mL) was heated to 70° C. for 3 hours. The mixture was cooled to room temperature, diluted with EtOAc and washed with water. The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness. The residue was purified by FCC (0-100% EtOAc/Heptane) followed by reverse-phase HPLC (acetonitrile/water+TFA) to provide the title compound as a yellow solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 1.69-1.81 (m, 9H), 7.04-7.16 (m, 2H), 7.27-7.37 (m, 3H), 7.39-7.44 (m, 2H), 7.49 (s, 2H), 7.53-7.61 (m, 2H), 7.74-7.86 (m, 2H), 8.25 (d, J=8.6 Hz, 1H), 8.28-8.43 (m, 2H), 8.71 (d, J=4.5 Hz, 1H), 8.93 (s, 1H). MS (ESI): mass calcd. for C 33 H 28 Cl 2 N 2 O, 538.2; m/z found, 539.2 [M+H] + .

›Example 28: (3-tert-Butyl-2,4-difuran-2-ylquinolin-6-yl)(3-chlorophenyl)pyridin-3-ylmethanol.TFA

The title compound was prepared using furan-2-ylboronic acid in place of (E)-styrylboronic acid using the procedure described for Example 27. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 1.04-1.21 (m, 9H), 6.40 (d, J=2.9 Hz, 1H), 6.48 (br. s., 1H), 6.55-6.69 (m, 1H), 6.85-7.01 (m, 2H), 7.07 (br. s., 1H), 7.29 (br. s., 3H), 7.49 (s, 1H), 7.55-7.77 (m, 3H), 8.17 (d, J=8.6 Hz, 2H), 8.80 (br. s., 2H). MS (ESI): mass calcd. for C 33 H 27 ClN 2 O 3 , 534.2; m/z found, 535.2 [M+H] + .

›Example 29: (3-Chlorophenyl)(2,4-dichloro-3-cyclohexylquinolin-6-yl)pyridin-3-ylmethanol.TFA · 1 of 2

To a solution of 6-bromo-2,4-dichloro-3-cyclohexylquinoline (710 mg, 1.98 mmol, Intermediate 19: step b) and (3-chlorophenyl)(pyridin-3-yl)methanone (474 mg, 2.18 mmol) in THF (31 mL) at −78° C. was added n-BuLi (1.6 M in hexanes, 1.61 mL, 2.57 mmol). The resulting solution was stirred at −78° C. for 10 minutes, then allowed to warm to room temperature. The mixture was quenched with saturated aqueous NH 4 Cl, the layers separated, and the aqueous further extracted with DCM. The organics were combined, dried, filtered and concentrated to dryness. The residue was purified by FCC (0-80% EtOAc/heptane) followed by reverse-phase HPLC (acetonitrile/water+TFA) to provide the title compound as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 1.28-1.52 (m, 3H), 1.58-1.83 (m, 3H), 1.93 (s, 1H), 1.90 (s, 1H), 2.26-2.53 (m, 2H), 3.67 (br. s., 1H), 7.11 (d, J=7.6 Hz, 1H), 7.28 (s, 1H), 7.32-7.42 (m, 2H), 7.57 (dd, J=8.8, 2.3 Hz, 1H), 7.77 (dd, J=8.1, 5.6 Hz, 1H), 7.99 (d, J=8.6 Hz, 1H), 8.12-8.25 (m, 2H), 8.80 (d, J=4.0 Hz, 1H), 8.94 (d, J=2.0 Hz, 1H). MS (ESI): mass calcd. for C 27 H 23 Cl 3 N 2 O, 496.1; m/z found, 496.8 [M+H] + .

Example 30: (4-Chloro-3-cyclohexyl-2-pyrimidin-5-ylquinolin-6-yl)(3-chlorophenyl)pyridin-3-ylmethanol

A mixture of (3-chlorophenyl)(2,4-dichloro-3-cyclohexylquinolin-6-yl)pyridin-3-ylmethanol (85 mg, 0.14 mmol, Example 29), pyrimidin-5-ylboronic acid (20 mg, 0.16 mmol), PdCl 2 (dppf) (10 mg, 0.014 mmol) and K 2 CO 3 (38 mg, 0.27 mmol) in 1,4-dioxane (10 mL) and water (2 mL) was heated to 70° C. for 3 hours. The mixture was cooled to room temperature, diluted with EtOAc and washed with water. The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness. The residue was purified by FCC (0-10% MeOH/EtOAc) to provide the title compound as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 1.08-1.31 (m, 3H), 1.68 (br. s., 2H), 1.66 (br. s., 1H), 1.82 (br. s., 1H), 1.79 (br. s., 1H), 2.32 (br. s., 2H), 2.98 (br. s., 1H), 7.19 (d, J=7.1 Hz, 1H), 7.28-7.35 (m, 3H), 7.37 (s, 1H), 7.62-7.74 (m, 2H), 8.03 (d, J=8.6 Hz, 1H), 8.33 (d, J=2.0 Hz, 1H), 8.51-8.63 (m, 2H), 8.86 (s, 2H), 9.28-9.38 (m, 1H). MS (ESI): mass calcd. for C 31 H 26 Cl 2 N 4 O, 540.1; m/z found, 540.9 [M+H] + .

Example 31: [2-Azetidin-1-yl-3-(benzyloxy)-4-chloroquinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA

To a sealed tube was added (3-(benzyloxy)-2,4-dichloroquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanol (200 mg, 0.36 mmol, Example 167), azetidine (20.4 mg, 0.36 mmol) and dimethylformamide (1 mL). The reaction vessel was sealed and heated to 60° C. for 2 hours. One drop of azetidine was then added and the vessel sealed and stirred at 60° C. for 2 hours. Additional azetidine (one drop) was added and the mixture stirred for another 2 hours at 60° C. The reaction was cooled to room temperature, diluted with EtOAc and washed with water five times. The organics were dried (MgSO 4 ), filtered and concentrated to dryness. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.1% TFA) to provide the title compound as a white solid. 1 H NMR (400 MHz, CD 3 OD) δ ppm 9.02 (s, 1H), 8.80-8.77 (m, 1H), 8.17 (d, J=2.0 Hz, 1H), 8.11-8.07 (m, 1H), 7.90 (d, J=8.3 Hz, 1H), 7.87-7.83 (m, 1H), 7.70-7.66 (m, 1H), 7.56-7.52 (m, 2H), 7.45-7.39 (m, 3H), 7.08 (s, 1H), 5.18 (s, 2H), 4.56 (t, J=7.8 Hz, 4H), 3.69 (s, 3H), 2.55-2.46 (m, 2H). MS (ESI): mass calcd. for C 30 H 25 ClF 3 N 5 O 2 , 579.2; m/z found, 580.2 [M+H] + .

Example 32: {3-[2-(1-Acetylpiperidin-4-yl)ethyl]-4-chloro-2-methoxyquinolin-6-yl}(1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a solution of (4-chloro-2-methoxy-3-(2-(piperidin-4-yl)ethyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanol (50 mg, 0.089 mmol, Example 174) in DCM (1.7 mL) was added Et 3 N (14 μL, 0.098 mmol) and the solution cooled to 0° C. Then, acetic anhydride (9 μL, 0.094 mmol) was added dropwise and the mixture stirred at 0° C. for 30 minutes. The solution was diluted with DCM and washed with saturated aqueous NaHCO 3 . The organics were dried (MgSO 4 ), filtered and concentrated to dryness, and the residue was purified by reverse-phase HPLC (acetonitrile/water+0.1% TFA). The acidic fractions were neutralized by diluting with EtOAc and washing with saturated aqueous NaHCO 3 . The organics were concentrated to dryness, dissolved in 1/1 acetonitrile/water and lyophilized to provide the title compound as a white solid. 1 H NMR (500 MHz, DMSO-d 6 ) δ ppm 8.78-8.75 (m, 1H), 8.09 (d, J=2.1 Hz, 1H), 7.95-7.90 (m, 2H), 7.83 (d, J=8.8 Hz, 1H), 7.74 (s, 1H), 7.56 (dd, J=8.6, 2.1 Hz, 1H), 7.43 (s, 1H), 6.22 (s, 1H), 4.40-4.33 (m, 1H), 4.04 (s, 3H), 3.84-3.78 (m, 1H), 3.34 (s, 3H), 3.04-2.97 (m, 1H), 2.92-2.86 (m, 2H), 1.99 (s, 3H), 1.82-1.74 (m, 2H), 1.58-1.51 (m, 1H), 1.50-1.44 (m, 2H), 1.27-0.92 (m, 3H). MS (ESI): mass calcd. for C 30 H 31 ClF 3 N 5 O 3 , 601.2; m/z found, 602.2 [M+H] + .

Example 33: [4-Chloro-2-methoxy-3-(1-methyl-1,2,3,6-tetrahydropyridin-4-yl)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA

A mixture of 4-chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methyl)-2-methoxyquinolin-3-yl trifluoromethanesulfonate (40 mg, 0.067 mmol, Intermediate 39), 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1,2,3,6-tetrahydropyridine (23 mg, 0.1 mmol), PdCl 2 (dppf) (5 mg, 0.007 mmol) and K 2 CO 3 (9 mg, 0.067 mmol) was sparged with nitrogen three times. To this mixture was added 1,4-dioxane (1.1 mL) and water (0.17 mL) and the suspension purged with nitrogen. The resulting solution was heated to 85° C. for 18 hours. The reaction was allowed to cool to room temperature and concentrated to dryness. The residue was dissolved in DMSO, filtered and purified by reverse-phase HPLC (acetonitrile/water+0.1% TFA) to provide the title compound. 1 H NMR (400 MHz, CD 3 OD) δ ppm 8.96 (s, 1H), 8.79 (d, J=2.2 Hz, 1H), 8.23 (d, J=2.4 Hz, 1H), 8.11-8.06 (m, 1H), 7.95 (d, J=8.8 Hz, 1H), 7.89 (d, J=8.3 Hz, 1H), 7.74-7.69 (m, 1H), 7.05-7.01 (m, 1H), 5.84-5.78 (m, 1H), 4.19-4.09 (m, 1H), 4.08 (s, 3H), 3.96-3.79 (m, 1H), 3.75-3.63 (m, 4H), 3.55-3.40 (m, 1H), 3.05 (s, 3H), 2.90-2.74 (m, 1H), 2.64-2.51 (m, 1H). MS (ESI): mass calcd. for C 27 H 25 ClF 3 N 5 O 2 , 543.2; m/z found, 544.2 [M+H] + .

›Example 29: (3-Chlorophenyl)(2,4-dichloro-3-cyclohexylquinolin-6-yl)pyridin-3-ylmethanol.TFA · 2 of 2

Example 34a: [4-Chloro-2-methoxy-3-(tetrahydrofuran-2-ylmethoxy)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

A mixture of 6-(bis(1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)-4-chloro-2-methoxyquinolin-3-ol (150 mg, 0.35 mmol, Intermediate 33), tetrahydrofurfuryl alcohol (103 μL, 1.05 mmol) and PPh 3 (276 mg, 1.05 mmol) in THF (0.7 mL) was sonicated to mix the reagents. While sonicating, DIAD (218 μL, 1.05 mmol) was added dropwise and the mixture was sonicated for 15 minutes. The reaction was concentrated to dryness and purified by FCC (1-10% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to afford the title compound as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.15-8.12 (m, 1H), 7.67 (d, J=8.7 Hz, 1H), 7.35-7.30 (m, 1H), 6.18 (s, 2H), 4.84 (s, 1H), 4.37-4.30 (m, 1H), 4.25-4.20 (m, 1H), 4.14-4.11 (m, 4H), 3.99-3.93 (m, 1H), 3.88-3.81 (m, 1H), 3.42-3.39 (m, 6H), 2.31 (s, 6H), 2.16-2.09 (m, 1H), 2.02-1.91 (m, 3H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 4 , 511.2; m/z found, 512.3 [M+H] + . [4-Chloro-2-methoxy-3-(tetrahydrofuran-2-ylmethoxy)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% EtOH (0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 34b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.11-8.09 (m, 1H), 7.74 (d, J=8.7 Hz, 1H), 7.39-7.35 (m, 1H), 6.29-6.25 (m, 2H), 4.35-4.31 (m, 1H), 4.22-4.18 (m, 1H), 4.13 (s, 3H), 4.12-4.09 (m, 1H), 3.98-3.94 (m, 1H), 3.86-3.82 (m, 1H), 3.44 (d, J=1.9 Hz, 6H), 3.43-3.40 (m, 1H), 2.36 (s, 6H), 2.15-2.08 (m, 1H), 2.03-1.98 (m, 1H), 1.96-1.91 (m, 2H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 4 , 511.2; m/z found, 512.1 [M+H] + and the second eluting enantiomer was Example 34c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.13-8.10 (m, 1H), 7.73 (d, J=8.5 Hz, 1H), 7.37-7.35 (m, 1H), 6.27-6.23 (m, 2H), 4.35-4.30 (m, 1H), 4.22-4.19 (m, 1H), 4.13 (s, 3H), 4.12-4.09 (m, 1H), 3.98-3.94 (m, 1H), 3.86-3.82 (m, 1H), 3.64 (s, 1H), 3.43 (d, J=2.2 Hz, 6H), 2.35 (s, 6H), 2.15-2.08 (m, 1H), 2.03-1.98 (m, 1H), 1.97-1.91 (m, 2H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 4 , 511.2; m/z found, 512.1 [M+H] + .

›Example 35: (4-Chloro-2,3-dimethoxyquinolin-6-yl) [bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol · 1 of 3

A mixture of 6-(bis(1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)-4-chloro-2-methoxyquinolin-3-ol (175 mg, 0.41 mmol, Intermediate 33), 2,2-difluorocyclopropylmethanol (57 μL, 0.67 mmol) and PPh 3 (161 mg, 0.61 mmol) in THF (0.8 mL) was sonicated to mix the reagents. While sonicating, DIAD (127 μL, 0.61 mmol) was added dropwise and the mixture was sonicated for 15 minutes. Additional 2,2-difluorocyclopropylmethanol (52 μL, 0.61 mmol), PPh 3 (161 mg, 0.61 mmol) and DIAD (127 μL, 0.61 mmol) were added and sonication continued for 15 minutes. HPLC indicated two major products, the desired cyclopropyl product as well as the methyl ether by-product. The reaction was concentrated to dryness and purified by FCC (1-10% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to afford the title compound. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.09 (d, J=2.2 Hz, 1H), 7.76 (d, J=8.7 Hz, 1H), 7.40 (dd, J=8.6, 2.2 Hz, 1H), 6.28 (s, 2H), 4.15 (s, 3H), 3.99 (s, 3H), 3.44 (s, 6H), 2.36 (s, 6H). MS (ESI): mass calcd. for C 22 H 24 ClN 5 O 3 , 441.2; m/z found, 442.0 [M+H] + .

Example 36a: {4-Chloro-2-methoxy-3-[(3-methyloxetan-3-yl)methoxy]quinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A mixture of 4-chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinolin-3-ol (200 mg, 0.47 mmol, Intermediate 31), 3-methyl-3-oxetanemethanol (71 μL, 0.7 mmol) and PPh 3 (185 mg, 0.7 mmol) in THF (0.94 mL) was sonicated to mix the reagents. While sonicating, DIAD (146 μL, 0.7 mmol) was added dropwise and the mixture was sonicated for 15 minutes. The reaction was concentrated to dryness and purified by FCC (1-10% MeOH/DCM) to afford the title compound as a cloudy white oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.00 (d, J=2.2 Hz, 1H), 7.83 (d, J=8.7 Hz, 1H), 7.34-7.31 (m, 1H), 6.99 (s, 1H), 6.95 (s, 2H), 4.74 (d, J=6.0 Hz, 2H), 4.50 (d, J=6.0 Hz, 2H), 4.20 (s, 2H), 4.15 (s, 3H), 3.95 (s, 3H), 3.30 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 1.53-1.52 (m, 3H). MS (ESI): mass calcd. for C 26 H 28 ClN 5 O 4 , 509.2; m/z found, 510.5 [M+H] + . {4-Chloro-2-methoxy-3-[(3-methyloxetan-3-yl)methoxy]quinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD 5 μM 250×30 mm, Mobile phase: 80% CO 2 , 20% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 36b: 1 H NMR (400 MHz, CD 3 OD) δ ppm 8.07-8.05 (m, 1H), 7.86 (d, J=8.6 Hz, 1H), 7.50-7.45 (m, 1H), 7.12-7.06 (m, 2H), 6.97 (s, 1H), 4.82-4.79 (m, 2H), 4.61 (s, 1H), 4.50-4.45 (m, 2H), 4.19 (s, 2H), 4.15 (s, 3H), 3.95 (s, 3H), 2.52 (s, 3H), 2.35 (s, 3H), 1.50 (s, 3H). MS (ESI): mass calcd. for C 26 H 28 ClN 5 O 4 , 509.2; m/z found, 510.1 [M+H] + and the second eluting enantiomer was Example 36c: 1 H NMR (400 MHz, CD 3 OD) δ ppm 8.07-8.05 (m, 1H), 7.86 (d, J=8.8 Hz, 1H), 7.49-7.45 (m, 1H), 7.12-7.06 (m, 2H), 6.97-6.96 (m, 1H), 4.81-4.79 (m, 2H), 4.61 (s, 1H), 4.49-4.46 (m, 2H), 4.19 (s, 2H), 4.15 (s, 3H), 3.95 (s, 3H), 2.51 (s, 3H), 2.35 (s, 3H), 1.50 (s, 3H). MS (ESI): mass calcd. for C 26 H 28 ClN 5 O 4 , 509.2; m/z found, 510.1 [M+H] + .

Example 37a: [4-Chloro-2-methoxy-3-(2-morpholin-4-ylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A mixture of 4-chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinolin-3-ol (200 mg, 0.47 mmol, Intermediate 31), 4-(2-hydroxyethyl)-morpholine (87 μL, 0.7 mmol) and PPh 3 (185 mg, 0.7 mmol) in THF (1.88 mL) was cooled to 0° C. Then DIAD (146 μL, 0.7 mmol) was added dropwise and the mixture was warmed to room temperature and stirred for 30 minutes. The reaction was concentrated to dryness and purified by FCC (1-10% MeOH/DCM) to afford the title compound as a clear colorless oil. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.01 (d, J=2.2 Hz, 1H), 7.79 (d, J=8.7 Hz, 1H), 7.34-7.30 (m, 1H), 6.97-6.91 (m, 2H), 6.89 (s, 1H), 4.55 (s, 1H), 4.27-4.24 (m, 2H), 4.13 (s, 3H), 3.92 (s, 3H), 3.72-3.69 (m, 2H), 3.69-3.65 (m, 4H), 3.62-3.58 (m, 1H), 2.86-2.83 (m, 2H), 2.53 (s, 3H), 2.51-2.49 (m, 1H), 2.35 (s, 3H). MS (ESI): mass calcd. for C 27 H 31 ClN 6 O 4 , 538.2; m/z found, 539.1 [M+H] + . [4-Chloro-2-methoxy-3-(2-morpholin-4-ylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The second eluting enantiomer was Example 37c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 7.98 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.8 Hz, 1H), 7.35-7.31 (m, 1H), 6.98 (s, 1H), 6.95 (s, 2H), 4.27 (t, J=5.5 Hz, 2H), 4.13 (s, 3H), 3.94 (s, 3H), 3.71-3.67 (m, 4H), 3.24 (s, 1H), 2.87-2.83 (m, 2H), 2.61-2.56 (m, 4H), 2.56 (s, 3H), 2.40 (s, 3H). MS (ESI): mass calcd. for C 27 H 31 ClN 6 O 4 , 538.2; m/z found, 539.1 [M+H] + .

Example 38a: [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA

To a sealed tube was added [2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA (182 mg, 0.31 mmol, Example 42), azetidine (107 μL, 1.55 mmol) and dimethylformamide (1.6 mL). The reaction vessel was sealed and heated in a 100° C. oil bath. After overnight heating, the vessel was cooled and the contents concentrated to dryness. The residue was dissolved in EtOAc (25 mL) and washed with saturated aqueous ammonium chloride (2×20 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a cream-colored foam. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.04 (d, J=8.8 Hz, 1H), 7.91 (d, J=2.1 Hz, 1H), 7.49-7.46 (m, 1H), 7.44-7.41 (m, 1H), 7.36 (d, J=8.3 Hz, 1H), 6.99 (s, 1H), 4.94-4.89 (m, 1H), 4.78-4.73 (m, 4H), 3.95 (s, 3H), 3.41-3.39 (m, 1H), 2.78 (s, 3H), 2.67 (s, 3H), 2.62-2.55 (m, 2H), 1.43-1.40 (m, 6H). MS (ESI): mass calcd. for C 26 H 29 ClN 6 O 2 , 492.2; m/z found, 493.0 [M+H] + . [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) followed by FCC (0.4% NH 4 OH, 96% DCM, 4% MeOH) to give 2 enantiomers. The first eluting enantiomer was Example 38b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 7.85 (d, J=2.2 Hz, 1H), 7.67 (d, J=8.8 Hz, 1H), 7.23-7.19 (m, 1H), 7.01-6.97 (m, 2H), 6.95-6.91 (m, 1H), 4.69-4.63 (m, 1H), 4.32-4.28 (m, 4H), 3.92 (s, 3H), 3.48 (s, 1H), 2.55 (s, 3H), 2.42-2.34 (m, 5H), 1.34 (d, J=6.1 Hz, 6H). MS (ESI): mass calcd. for C 26 H 29 ClN 6 O 2 , 492.2; m/z found, 493.2 [M+H] + and the second eluting enantiomer was Example 38c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 7.85 (d, J=2.2 Hz, 1H), 7.67 (d, J=8.8 Hz, 1H), 7.21 (dd, J=8.8, 2.2 Hz, 1H), 7.00-6.97 (m, 2H), 6.95-6.91 (m, 1H), 4.69-4.63 (m, 1H), 4.32-4.27 (m, 4H), 3.92 (s, 3H), 3.57 (s, 1H), 2.54 (s, 3H), 2.41-2.34 (m, 5H), 1.34 (d, J=6.1 Hz, 6H). MS (ESI): mass calcd. for C 26 H 29 ClN 6 O 2 , 492.2; m/z found, 493.2 [M+H] + .

›Example 35: (4-Chloro-2,3-dimethoxyquinolin-6-yl) [bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol · 2 of 3

Example 39: [2,4-Dichloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a solution of 6-bromo-2,4-dichloro-3-(2,2,2-trifluoroethoxy)quinoline (288 mg, 0.77 mmol, Intermediate 23: step d) and (1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone (196 mg, 0.77 mmol, Intermediate 10: step c) in THF (18.3 mL) at −40° C. was added n-BuLi (1.23 M in hexanes, 624 μL, 0.77 mmol) dropwise. The resulting dark yellow solution was stirred at −40° C. for 30 minutes. n-BuLi (1.23 M in hexanes, 624 μL, 0.77 mmol) was added and the mixture stirred at −40° C. for 30 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (7 mL), water (15 mL) and EtOAc (20 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (20 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow oil. The crude material was purified by FCC (0.5-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a light yellow oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.87-8.85 (m, 1H), 8.33-8.30 (m, 1H), 8.24-8.22 (m, 1H), 8.08 (d, J=8.8 Hz, 1H), 7.93-7.90 (m, 1H), 7.72 (d, J=8.2 Hz, 1H), 7.71-7.67 (m, 1H), 6.73-6.71 (m, 1H), 4.58-4.51 (m, 2H), 3.63 (s, 3H). MS (ESI): mass calcd. for C 22 H 14 Cl 2 F 6 N 4 O 2 , 550.0; m/z found, 552.9 [M+H] + .

Example 40: [2,4-Dichloro-3-(cyclopropylmethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA

To a solution of 6-bromo-2,4-dichloro-3-(cyclopropylmethoxy)quinoline (263 mg, 0.76 mmol, Intermediate 24: step b) and (1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone (193 mg, 0.76 mmol, Intermediate 10: step c) in THF (18 mL) at −40° C. was added n-BuLi (1.23 M in hexanes, 616 μL, 0.76 mmol) dropwise. The resulting orange solution was stirred at −40° C. for 30 minutes. n-BuLi (1.23 M in hexanes, 542 μL, 0.67 mmol) was added and the mixture stirred at −40° C. for 2 hours, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (7 mL), water (15 mL) and EtOAc (20 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (20 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow foam. The crude material was purified by FCC (0.5-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.80-8.77 (m, 1H), 8.70 (s, 1H), 8.19-8.17 (m, 1H), 8.01 (d, J=8.8 Hz, 1H), 7.97-7.94 (m, 1H), 7.71 (d, J=8.3 Hz, 1H), 7.64-7.60 (m, 1H), 6.79 (s, 1H), 4.01 (d, J=7.3 Hz, 2H), 3.61 (s, 3H), 1.43-1.36 (m, 1H), 0.68-0.63 (m, 2H), 0.40-0.34 (m, 2H). MS (ESI): mass calcd. for C 24 H 19 Cl 2 F 3 N 4 O 2 , 522.1; m/z found, 523.0 [M+H] + .

Example 41: [2,4-Dichloro-3-(1-methylethoxy)quinolin-6-yl](1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA

n-BuLi (1.23 M in hexanes, 447 μL, 0.55 mmol) was added dropwise to a stirred slurry of 1-methyl-1,2,3-triazole (46 mg, 0.55 mmol) in THF (1 mL) at −40° C. under nitrogen. After stirring for 30 minutes at −40° C., the mixture was treated dropwise with a solution of (2,4-dichloro-3-isopropoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (104 mg, 0.27 mmol, Intermediate 25) in THF (1 mL). The reaction was allowed to warm to room temperature over 1 hour. The reaction was then quenched with saturated aqueous NH 4 Cl. The mixture was poured into a separatory funnel and extracted with DCM (2×25 mL). The organics were combined, washed with brine, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford an orange-brown foam. The crude material was purified by FCC (1-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.23-8.20 (m, 1H), 7.98 (d, J=8.8 Hz, 1H), 7.52-7.48 (m, 1H), 7.24 (s, 1H), 6.40 (s, 1H), 4.80-4.73 (m, 1H), 3.92 (s, 3H), 3.63 (s, 3H), 2.58 (s, 3H), 1.44-1.41 (m, 6H). MS (ESI): mass calcd. for C 21 H 22 Cl 2 N 6 O 2 , 460.1; m/z found, 461.1 [M+H] + .

Example 42: [2,4-Dichloro-3-(1-methylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA

To a solution of 6-bromo-2,4-dichloro-3-isopropoxyquinoline (150 mg, 0.45 mmol, Intermediate 20: step c) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (97 mg, 0.45 mmol, Intermediate 11: step b) in THF (10.7 mL) at −40° C. was added n-BuLi (1.23 M in hexanes, 364 μL, 0.45 mmol) dropwise. The resulting red-orange solution was stirred at −40° C. for 30 minutes. Additional n-BuLi (1.23 M in hexanes, 91 μL, 0.11 mmol) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (22 mg, 0.1 mmol, Intermediate 11: step b) were added and the mixture stirred at −40° C. for 30 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (4 mL), water (25 mL) and EtOAc (35 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (35 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow oil. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.10-8.08 (m, 1H), 8.07 (d, J=8.8 Hz, 1H), 7.55 (d, J=8.3 Hz, 1H), 7.47-7.44 (m, 1H), 7.37 (d, J=8.4 Hz, 1H), 7.09 (s, 1H), 4.83-4.77 (m, 1H), 3.97 (s, 3H), 2.77 (s, 3H), 2.66 (s, 3H), 1.47-1.44 (m, 6H). MS (ESI): mass calcd. for C 23 H 23 Cl 2 N 5 O 2 , 471.1; m/z found, 472.0 [M+H] + .

Example 43: [2,4-Dichloro-3-(1-methylethoxy)quinolin-6-yl](2,4-dimethyl-1,3-thiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA

›Example 35: (4-Chloro-2,3-dimethoxyquinolin-6-yl) [bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol · 3 of 3

To a solution of 6-bromo-2,4-dichloro-3-isopropoxyquinoline (250 mg, 0.75 mmol, Intermediate 20: step c) and (2,4-dimethylthiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (166 mg, 0.75 mmol, Intermediate 12: step b) in THF (17.8 mL) at −40° C. was added n-BuLi (1.23 M in hexanes, 607 μL, 0.75 mmol) dropwise. The resulting red-orange solution was stirred at −40° C. for 30 minutes. Additional n-BuLi (1.23 M in hexanes, 152 μL, 0.19 mmol) and (2,4-dimethylthiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (41.5 mg, 0.19 mmol, Intermediate 12: step b) were added and the mixture stirred at −40° C. for 30 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (7 mL), water (25 mL) and EtOAc (35 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (35 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow foam. The crude material was purified by FCC (0.5-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.19 (d, J=2.1 Hz, 1H), 8.03 (d, J=8.8 Hz, 1H), 7.63 (dd, J=8.9, 2.2 Hz, 1H), 7.27-7.25 (m, 1H), 4.83-4.75 (m, 1H), 3.92 (s, 3H), 2.70 (s, 3H), 2.20 (s, 3H), 1.45 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 21 H 21 Cl 2 N 5 O 2 S, 477.1; m/z found, 478.0 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 1 of 15

n-BuLi (1.23 M in hexanes, 364 μL, 0.45 mmol) was added dropwise to a stirred solution of 6-bromo-2,4-dichloro-3-isopropoxyquinoline (150 mg, 0.45 mmol, Intermediate 20: step c) in THF (8 mL) at −40° C. under nitrogen. After stirring for 5 minutes at −40° C., the mixture was treated dropwise with a solution of 1-(4-benzoylpiperidin-1-yl)ethanone (104 mg, 0.45 mmol, Intermediate 7) in THF (3 mL). The flask was rinsed with THF (3 mL), which was then added to the reaction. The resulting brown solution was stirred at −40° C. for 15 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (5 mL), water (20 mL) and EtOAc (25 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (25 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow oil. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.36-8.31 (m, 1H), 7.93-7.88 (m, 1H), 7.74-7.66 (m, 1H), 7.56-7.51 (m, 2H), 7.38-7.32 (m, 2H), 7.25-7.22 (m, 1H), 4.79-4.73 (m, 1H), 4.71-4.63 (m, 1H), 3.89-3.79 (m, 1H), 3.30-3.20 (m, 1H), 3.17-3.05 (m, 2H), 2.83-2.75 (m, 1H), 2.68-2.56 (m, 1H), 2.09-2.06 (m, 3H), 1.78-1.70 (m, 1H), 1.52-1.47 (m, 1H), 1.44 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 26 H 28 Cl 2 N 2 O 3 , 486.1; m/z found, 487.0 [M+H] + .

Example 45: (4-Chlorophenyl)[2,4-dichloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)methanol

To a suspension of 6-bromo-2,4-dichloro-3-(2,2,2-trifluoroethoxy)quinoline (250 mg, 0.67 mmol, Intermediate 23: step d) and (4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanone (147 mg, 0.67 mmol, Intermediate 22: step b) in THF (16 mL) at −40° C. was added n-BuLi (1.23 M in hexanes, 542 μL, 0.67 mmol) dropwise. The resulting dark yellow-brown solution was stirred at −40° C. for 30 minutes. n-BuLi (1.23 M in hexanes, 542 μL, 0.67 mmol) was added and the mixture stirred at −40° C. for 30 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (6 mL), water (25 mL) and EtOAc (35 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (35 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to give a light orange foam. The crude material was purified by FCC (0.5-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+TFA) to afford the title compound as a clear colorless oil. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.67-8.64 (m, 1H), 8.17 (d, J=2.1 Hz, 1H), 8.07 (d, J=8.8 Hz, 1H), 7.64 (dd, J=8.9, 2.1 Hz, 1H), 7.44-7.39 (m, 2H), 7.29-7.26 (m, 2H), 6.77-6.74 (m, 1H), 4.58-4.50 (m, 2H), 3.67 (s, 3H). MS (ESI): mass calcd. for C 22 H 15 Cl 3 F 3 N 3 O 2 , 515.0; m/z found, 516.8 [M+H] + .

Example 46: (2,4-Dichloro-3-ethoxyquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA

To a solution of 6-bromo-2,4-dichloro-3-ethoxyquinoline (100 mg, 0.31 mmol, Intermediate 21: step b) and (1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone (80 mg, 0.31 mmol, Intermediate 10: step c) in THF (7.4 mL) at −40° C. was added n-BuLi (1.23 M in hexanes, 253 μL, 0.31 mmol) dropwise. The resulting dark yellow solution was stirred at −40° C. for 30 minutes. n-BuLi (1.23 M in hexanes, 253 μL, 0.31 mmol) was added and the mixture stirred at −40° C. for 30 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (1.5 mL), water (10 mL) and EtOAc (20 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (20 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow foam. The crude material was purified by FCC (0.5-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.90-8.87 (s, 1H), 8.59-8.55 (m, 1H), 8.23-8.20 (m, 1H), 8.06-8.02 (d, J=8.8 Hz, 1H), 7.97-7.93 (m, 1H), 7.73-7.69 (d, J=8.3 Hz, 1H), 7.66-7.63 (m, 1H), 6.85-6.82 (s, 1H), 4.27-4.22 (m, 2H), 3.66-3.63 (s, 3H), 1.56-1.53 (m, 3H). MS (ESI): mass calcd. for C 22 H 17 Cl 2 F 3 N 4 O 2 , 496.1; m/z found, 498.0 [M+H] + .

Example 47: (4-Chlorophenyl)(2,4-dichloro-3-ethoxyquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)methanol.TFA

To a suspension of 6-bromo-2,4-dichloro-3-ethoxyquinoline (250 mg, 0.78 mmol, Intermediate 21: step b) and (4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanone (172 mg, 0.78 mmol, Intermediate 22: step b) in THF (18.5 mL) at −40° C. was added n-BuLi (1.23 M in hexanes, 633 μL, 0.78 mmol) dropwise. The resulting dark yellow solution was stirred at −40° C. for 30 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (9 mL), water (50 mL) and EtOAc (100 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (100 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow oil. The crude material was purified by FCC (5-100% EtOAc/hexanes) followed by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.57-8.53 (s, 1H), 8.12-8.10 (m, 1H), 8.05-8.00 (m, 2H), 7.61-7.58 (m, 1H), 7.42-7.38 (m, 2H), 7.30-7.27 (d, J=8.7 Hz, 2H), 6.70-6.68 (s, 1H), 4.28-4.22 (m, 2H), 3.66-3.64 (s, 3H), 1.57-1.53 (m, 3H). MS (ESI): mass calcd. for C 22 H 18 Cl 3 N 3 O 2 , 461.0; m/z found, 462.0 [M+H] + .

Example 48: [2,4-Dichloro-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA

To a solution of 6-bromo-2,4-dichloro-3-isopropoxyquinoline (300 mg, 0.9 mmol, Intermediate 20: step c) and (1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone (229 mg, 0.9 mmol, Intermediate 10: step c) in THF (21 mL) at −40° C. was added n-BuLi (1.23 M in hexanes, 728 μL, 0.9 mmol) dropwise. The resulting orange solution was stirred at −40° C. for 30 minutes. n-BuLi (1.23 M in hexanes, 728 μL, 0.9 mmol) was added and the mixture stirred at −40° C. for 30 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (7 mL), water (25 mL) and EtOAc (35 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (35 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow foam. The crude material was purified by FCC (0.5-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.91-8.87 (m, 1H), 8.58-8.53 (m, 1H), 8.23-8.19 (m, 1H), 8.06-8.02 (d, J=8.8 Hz, 1H), 7.98-7.93 (m, 1H), 7.73-7.70 (d, J=8.3 Hz, 1H), 7.66-7.61 (m, 1H), 6.84-6.81 (s, 1H), 4.82-4.75 (m, 1H), 3.67-3.63 (s, 3H), 1.46-1.43 (m, 6H). MS (ESI): mass calcd. for C 23 H 19 Cl 2 F 3 N 4 O 2 , 510.1; m/z found, 512.1 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 2 of 15

Example 49a: (4-Chlorophenyl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)methanol.TFA

To a solution of 6-bromo-2,4-dichloro-3-isopropoxyquinoline (75 mg, 0.22 mmol, Intermediate 20: step c) and (4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanone (49.4 mg, 0.22 mmol, Intermediate 22: step b) in THF (5.3 mL) at −40° C. was added n-BuLi (1.23 M in hexanes, 182 μL, 0.22 mmol) dropwise. The resulting dark yellow solution was stirred at −40° C. for 30 minutes. n-BuLi (1.23 M in hexanes, 182 μL, 0.22 mmol) was added and the mixture stirred at −40° C. for 30 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (1 mL), water (10 mL) and EtOAc (15 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (10 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a light yellow oil. The crude material was purified by FCC (0.5-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.49-8.43 (m, 1H), 8.13-8.10 (m, 1H), 8.01-7.97 (d, J=8.8 Hz, 1H), 7.63-7.59 (m, 1H), 7.39-7.35 (m, 2H), 7.33-7.29 (m, 2H), 6.62-6.58 (s, 1H), 4.80-4.75 (m, 1H), 3.64-3.60 (s, 3H), 1.46-1.43 (m, 6H). MS (ESI): mass calcd. for C 23 H 20 Cl 3 N 3 O 2 , 475.1; m/z found, 477.0 [M+H] + . (4-Chlorophenyl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)methanol was purified by achiral SFC (Stationary phase: CHIRALPAK OD-H column, Mobile phase: 90% CO 2 , 10% MeOH+0.2% iPrNH 2 ) followed by chiral SFC (Stationary phase: CHIRALPAK OJ-H column, Mobile phase: 87% CO 2 , 13% iPrOH+0.2% iPrNH 2 ) to give 2 enantiomers. The first eluting enantiomer was Example 49b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.17-8.15 (m, 1H), 7.96 (d, J=8.8 Hz, 1H), 7.64-7.60 (m, 1H), 7.51-7.48 (m, 1H), 7.36-7.30 (m, 4H), 6.51-6.47 (m, 1H), 4.79-4.74 (m, 1H), 3.42 (s, 3H), 3.01-2.97 (m, 1H), 1.44 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 23 H 20 Cl 3 N 3 O 2 , 475.1; m/z found, 477.0 [M+H] + and the second eluting enantiomer was Example 49c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.17-8.15 (m, 1H), 7.96 (d, J=8.8 Hz, 1H), 7.63-7.59 (m, 1H), 7.54-7.49 (m, 1H), 7.36-7.30 (m, 4H), 6.53-6.47 (m, 1H), 4.80-4.74 (m, 1H), 3.42 (s, 3H), 3.05-3.01 (m, 1H), 1.44 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 23 H 20 Cl 3 N 3 O 2 , 475.1; m/z found, 477.0 [M+H] + .

Example 50a: 1-({4-Chloro-6-[(2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl]-2-methoxyquinolin-3-yl}oxy)-2-methylpropan-2-ol

Step A. (3-(2-(benzyloxy)-2-methylpropoxy)-4-chloro-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol. A mixture of 4-chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinolin-3-ol (335 mg, 0.79 mmol, Intermediate 31), 2-benzyloxy-2-methylpropan-1-ol (214 μL, 1.18 mmol) and PPh 3 (310 mg, 1.18 mmol) in THF (1.57 mL) was sonicated to mix the reagents. While sonicating, DIAD (245 μL, 1.18 mmol) was added dropwise and the mixture was sonicated for 15 minutes. Additional 2-benzyloxy-2-methylpropan-1-ol (214 μL, 1.18 mmol), PPh 3 (310 mg, 1.18 mmol) and DIAD (245 μL, 1.18 mmol) were added and sonication continued for 45 minutes. Additional 2-benzyloxy-2-methylpropan-1-ol (214 μL, 1.18 mmol), PPh 3 (310 mg, 1.18 mmol) and DIAD (245 μL, 1.18 mmol) were added and sonication continued for 1 hour. Additional 2-benzyloxy-2-methylpropan-1-ol (214 μL, 1.18 mmol), PPh 3 (310 mg, 1.18 mmol) and DIAD (245 μL, 1.18 mmol) were added and sonication continued for 30 minutes. Additional 2-benzyloxy-2-methylpropan-1-ol (214 μL, 1.18 mmol), PPh 3 (310 mg, 1.18 mmol) and DIAD (245 μL, 1.18 mmol) were added and sonication continued for 1 hour. The reaction was concentrated to dryness and purified twice by FCC (1-10% MeOH/DCM) to afford the title compound as a yellow oil. MS (ESI): mass calcd. for C 32 H 34 ClN 5 O 4 , 587.2; m/z found 588.2 [M+H] + .

Step B. To a solution of (3-(2-(benzyloxy)-2-methylpropoxy)-4-chloro-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol (216 mg, 0.37 mmol, step A) in MeOH (88 mL) was added 10% Pd/C (235 mg, 0.22 mmol). The reaction vessel was evacuated and then placed under an atmosphere of hydrogen for 21.5 hours. The mixture was then flushed with N 2 and filtered through a pad of Celite®. The Celite® was rinsed with MeOH and the filtrate was concentrated to dryness. The residue was then resubjected to the reaction conditions, utilizing the H-cube. The solution was run through the H-cube at 1 mL/minute and 40° C. for 3 hours. The solution was concentrated to dryness and the residue was purified by FCC (1-40% acetonitrile/DCM) followed by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to provide the title compound as a cream-colored solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.00 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.34-7.31 (m, 1H), 6.97 (s, 1H), 6.95 (s, 2H), 4.15 (s, 3H), 4.00 (s, 2H), 3.94 (s, 3H), 3.32 (s, 1H), 2.97 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 1.37 (s, 6H). MS (ESI): mass calcd. for C 25 H 28 ClN 5 O 4 , 497.2; m/z found, 498.1 [M+H] + . 1-({4-Chloro-6-[(2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl]-2-methoxyquinolin-3-yl}oxy)-2-methylpropan-2-ol was purified by chiral SFC (Stationary phase: CHIRALCEL OD-H 5 μM 250×20 mm, Mobile phase: 65% CO 2 , 35% iPrOH (0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 50b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.02-8.00 (m, 1H), 7.82-7.80 (m, 1H), 7.36-7.33 (m, 1H), 6.96 (s, 2H), 6.95 (s, 1H), 4.14 (s, 3H), 3.95 (s, 3H), 3.68 (d, J=6.8 Hz, 2H), 3.56 (s, 1H), 3.26-3.23 (m, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 1.41 (d, J=3.5 Hz, 6H). MS (ESI): mass calcd. for C 25 H 28 ClN 5 O 4 , 497.2; m/z found, 498.1 [M+H] + and the second eluting enantiomer was Example 50c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.02-8.00 (m, 1H), 7.83-7.81 (m, 1H), 7.36-7.33 (m, 1H), 6.97 (s, 1H), 6.96 (s, 2H), 4.14 (s, 3H), 3.95 (s, 3H), 3.68 (d, J=6.7 Hz, 2H), 3.41 (s, 1H), 3.24-3.21 (m, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 1.41 (d, J=3.9 Hz, 6H). MS (ESI): mass calcd. for C 25 H 28 ClN 5 O 4 , 497.2; m/z found, 498.1 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 3 of 15

Example 51a: (4-Chloro-2-methoxy-3-{[1-methylpiperidin-3-yl]oxy}quinolin-6-yl)[bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using 3-hydroxy-1-methyl-piperidine in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. MS (ESI): mass calcd. for C 27 H 33 ClN 6 O 3 , 524.2; m/z found, 525.3 [M+H] + . (4-Chloro-2-methoxy-3-{[1-methylpiperidin-3-yl]oxy}quinolin-6-yl)[bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol was purified by achiral SFC (Stationary phase: CYANO 6 μM 150×21.2 mm, Mobile phase: 85% CO 2 , 15% MeOH (0.3% iPrNH 2 )) followed by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 80% CO 2 , 20% EtOH (0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 51b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.10-8.07 (m, 1H), 7.76 (d, J=8.7 Hz, 1H), 7.41-7.37 (m, 1H), 6.29 (d, J=7.4 Hz, 2H), 4.48-4.41 (m, 1H), 4.12 (s, 3H), 3.46-3.44 (m, 6H), 3.15-3.09 (m, 1H), 3.08-2.99 (m, 1H), 2.71-2.62 (m, 1H), 2.37 (s, 6H), 2.36-2.29 (m, 3H), 2.17-2.00 (m, 2H), 1.95-1.86 (m, 1H), 1.64-1.59 (m, 2H). MS (ESI): mass calcd. for C 27 H 33 ClN 6 O 3 , 524.2; m/z found, 525.2 [M+H] + and the second eluting enantiomer was Example 51c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.17-8.14 (m, 1H), 7.66 (d, J=8.6 Hz, 1H), 7.36-7.29 (m, 1H), 6.20-6.10 (m, 2H), 5.61 (s, 1H), 4.48-4.38 (m, 1H), 4.11 (s, 3H), 3.39 (d, J=6.2 Hz, 6H), 3.09-3.01 (m, 1H), 2.68-2.61 (m, 1H), 2.33 (s, 3H), 2.29-2.26 (m, 6H), 2.13-2.04 (m, 2H), 1.94-1.85 (m, 2H), 1.65-1.55 (m, 2H). MS (ESI): mass calcd. for C 27 H 33 ClN 6 O 3 , 524.2; m/z found, 525.2 [M+H] + .

Example 52: {4-Chloro-2-methoxy-3-[(1-methylpyrrolidin-2-yl)methoxy]quinolin-6-yl}[bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using 3-hydroxy-1-methyl-piperidine in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. Purification was accomplished using achiral SFC (Stationary phase: CYANO 6 μM 150×21.2 mm, Mobile phase: 85% CO 2 , 15% MeOH (0.3% iPrNH 2 )). 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.10-8.07 (m, 1H), 7.76 (d, J=8.7 Hz, 1H), 7.40-7.37 (m, 1H), 6.29 (d, J=3.7 Hz, 2H), 4.13 (s, 3H), 4.10-4.05 (m, 1H), 3.45-3.44 (m, 6H), 3.16-3.10 (m, 1H), 3.09-3.05 (m, 1H), 2.71-2.62 (m, 1H), 2.52 (s, 3H), 2.37 (s, 6H), 2.33-2.26 (m, 1H), 2.11-2.03 (m, 1H), 1.99-1.90 (m, 1H), 1.90-1.83 (m, 1H), 1.82-1.74 (m, 1H). MS (ESI): mass calcd. for C 27 H 33 ClN 6 O 3 , 524.2; m/z found, 525.2 [M+H] + .

Example 53a: [4-Chloro-2-methoxy-3-(tetrahydrofuran-3-ylmethoxy)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using tetrahydro-3-furan-methanol in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.14-8.12 (m, 1H), 7.68 (d, J=8.7 Hz, 1H), 7.37-7.32 (m, 1H), 6.19 (s, 2H), 4.74-4.50 (m, 1H), 4.14-4.11 (m, 4H), 4.07-4.04 (m, 1H), 4.02-3.96 (m, 1H), 3.93-3.86 (m, 2H), 3.83-3.76 (m, 1H), 3.41 (s, 6H), 2.87-2.77 (m, 1H), 2.32 (s, 6H), 2.19-2.09 (m, 1H), 1.87-1.78 (m, 1H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 4 , 511.2; m/z found, 512.3 [M+H] + . [4-Chloro-2-methoxy-3-(tetrahydrofuran-3-ylmethoxy)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% EtOH (0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 53b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.11 (s, 1H), 7.73 (d, J=8.6 Hz, 1H), 7.39-7.35 (m, 1H), 6.25 (s, 2H), 4.14-4.11 (m, 4H), 4.05-4.02 (m, 1H), 4.00-3.96 (m, 1H), 3.93-3.86 (m, 2H), 3.82-3.78 (m, 1H), 3.73-3.64 (m, 1H), 3.43 (s, 6H), 2.86-2.79 (m, 1H), 2.35 (s, 6H), 2.18-2.10 (m, 1H), 1.85-1.79 (m, 1H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 4 , 511.2; m/z found, 512.1 [M+H] + and the second eluting enantiomer was Example 53c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.10 (s, 1H), 7.73 (d, J=8.6 Hz, 1H), 7.37 (d, J=8.7 Hz, 1H), 6.25 (s, 2H), 4.15-4.12 (m, 4H), 4.06-4.01 (m, 1H), 4.00-3.96 (m, 1H), 3.94-3.86 (m, 2H), 3.82-3.77 (m, 1H), 3.75-3.68 (m, 1H), 3.43 (s, 6H), 2.85-2.79 (m, 1H), 2.35 (s, 6H), 2.17-2.10 (m, 1H), 1.85-1.78 (m, 1H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 4 , 511.2; m/z found, 512.1 [M+H] + .

Example 54: 1-({6-[Bis(1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl]-4-chloro-2-methoxyquinolin-3-yl}oxy)-2-methylpropan-2-ol

The title compound was prepared using 2-methyl-1,2-propanediol in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.11 (d, J=2.1 Hz, 1H), 7.74 (d, J=8.7 Hz, 1H), 7.41 (dd, J=8.7, 2.1 Hz, 1H), 6.25 (s, 2H), 4.13 (s, 3H), 3.68 (s, 2H), 3.44 (s, 6H), 2.35 (s, 6H), 1.42-1.41 (m, 6H). MS (ESI): mass calcd. for C 25 H 30 ClN 5 O 4 , 499.2; m/z found, 500.1 [M+H] + .

Example 55a: [4-Chloro-2-methoxy-3-(oxetan-2-ylmethoxy)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using 2-hydroxymethyloxetane in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.17-8.14 (m, 1H), 7.66 (d, J=8.6 Hz, 1H), 7.35-7.30 (m, 1H), 6.15 (s, 2H), 5.47 (s, 1H), 5.19-5.12 (m, 1H), 4.76-4.66 (m, 2H), 4.41-4.30 (m, 2H), 4.14 (s, 3H), 3.40 (s, 6H), 2.89-2.78 (m, 2H), 2.28 (s, 6H). MS (ESI): mass calcd. for C 25 H 28 ClN 5 O 4 , 497.2; m/z found, 498.1 [M+H] + . [4-Chloro-2-methoxy-3-(oxetan-2-ylmethoxy)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% EtOH (0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 55b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.12-8.09 (m, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.40-7.36 (m, 1H), 6.27 (s, 2H), 5.18-5.12 (m, 1H), 4.75-4.66 (m, 2H), 4.39-4.28 (m, 2H), 4.14 (s, 3H), 3.44 (s, 6H), 3.41-3.37 (m, 1H), 2.87-2.79 (m, 2H), 2.36 (s, 6H). MS (ESI): mass calcd. for C 25 H 28 ClN 5 O 4 , 497.2; m/z found, 498.1 [M+H] + and the second eluting enantiomer was Example 55c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.12-8.10 (m, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.41-7.36 (m, 1H), 6.27 (s, 2H), 5.19-5.12 (m, 1H), 4.75-4.66 (m, 2H), 4.39-4.29 (m, 2H), 4.14 (s, 3H), 3.44 (s, 6H), 2.86-2.77 (m, 2H), 2.36 (s, 6H). MS (ESI): mass calcd. for C 25 H 28 ClN 5 O 4 , 497.2; m/z found, 498.1 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 4 of 15

Example 56a: {4-Chloro-3-[(2,2-difluorocyclopropyl)methoxy]-2-methoxyquinolin-6-yl}[bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using 2,2-difluorocyclopropylmethanol in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.18-8.15 (m, 1H), 7.65 (d, J=8.7 Hz, 1H), 7.37-7.32 (m, 1H), 6.18-6.11 (m, 2H), 5.66 (s, 1H), 4.32-4.17 (m, 2H), 4.13 (s, 3H), 3.39 (s, 6H), 2.29-2.26 (m, 6H), 2.21-2.14 (m, 1H), 1.65-1.55 (m, 1H), 1.39-1.30 (m, 1H). MS (ESI): mass calcd. for C 25 H 26 ClF 2 N 5 O 3 , 517.2; m/z found, 518.1 [M+H] + . {4-Chloro-3-[(2,2-difluorocyclopropyl)methoxy]-2-methoxyquinolin-6-yl}[bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 80% CO 2 , 20% EtOH (0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 56b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.13-8.09 (m, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.42-7.38 (m, 1H), 6.27 (s, 2H), 4.31-4.17 (m, 2H), 4.14 (s, 3H), 3.55-3.47 (m, 1H), 3.44 (s, 6H), 2.36 (s, 6H), 2.21-2.11 (m, 1H), 1.37-1.28 (m, 2H). MS (ESI): mass calcd. for C 25 H 26 ClF 2 N 5 O 3 , 517.2; m/z found, 518.1 [M+H] + and the second eluting enantiomer was Example 56c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.13-8.09 (m, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.42-7.37 (m, 1H), 6.26 (s, 2H), 4.30-4.18 (m, 2H), 4.14 (s, 3H), 3.73-3.56 (m, 1H), 3.44 (s, 6H), 2.36 (s, 6H), 2.21-2.12 (m, 1H), 1.36-1.29 (m, 2H). MS (ESI): mass calcd. for C 25 H 26 ClF 2 N 5 O 3 , 517.2; m/z found, 518.1 [M+H] + .

Example 57a: [4-Chloro-3-(1-cyclopropylethoxy)-2-methoxyquinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using alpha-methylcyclopropane methanol in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.13-8.11 (m, 1H), 7.73 (d, J=8.7 Hz, 1H), 7.39-7.35 (m, 1H), 6.25 (s, 2H), 4.21 (s, 1H), 4.10 (s, 3H), 3.91-3.83 (m, 1H), 3.44-3.42 (m, 6H), 2.32 (s, 6H), 1.49-1.46 (m, 3H), 1.22-1.16 (m, 1H), 0.54-0.42 (m, 2H), 0.21-0.13 (m, 2H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 3 , 495.2; m/z found, 496.2 [M+H] + . [4-Chloro-3-(1-cyclopropylethoxy)-2-methoxyquinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% EtOH (0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 57b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.09 (d, J=2.2 Hz, 1H), 7.77 (d, J=8.7 Hz, 1H), 7.40-7.36 (m, 1H), 6.31 (s, 2H), 4.11 (s, 3H), 3.94-3.84 (m, 1H), 3.47-3.44 (m, 6H), 3.00-2.97 (m, 1H), 2.37 (s, 6H), 1.47 (d, J=6.2 Hz, 3H), 0.55-0.41 (m, 2H), 0.22-0.12 (m, 2H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 3 , 495.2; m/z found, 496.2 [M+H] + and the second eluting enantiomer was Example 57c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.11-8.09 (m, 1H), 7.76 (d, J=8.6 Hz, 1H), 7.40-7.36 (m, 1H), 6.30 (s, 2H), 4.11 (s, 3H), 3.91-3.84 (m, 1H), 3.46-3.44 (m, 6H), 3.25-3.20 (m, 1H), 2.36 (s, 6H), 1.47 (d, J=6.2 Hz, 3H), 0.54-0.42 (m, 2H), 0.22-0.13 (m, 2H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 3 , 495.2; m/z found, 496.2 [M+H] + .

Example 58: {4-Chloro-3-[(1,3-dimethyl-1H-pyrazol-5-yl)methoxy]-2-methoxyquinolin-6-yl}[bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using (1,3-dimethyl-1H-pyrazol-5-yl)methanol in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.14-8.11 (m, 1H), 7.70 (d, J=8.7 Hz, 1H), 7.39-7.35 (m, 1H), 6.19 (s, 2H), 6.04 (s, 1H), 5.13 (s, 2H), 4.15 (s, 3H), 4.01 (s, 3H), 3.41 (s, 6H), 2.31 (s, 6H), 2.22 (s, 3H). MS (ESI): mass calcd. for C 27 H 30 ClN 7 O 3 , 535.2; m/z found, 536.1 [M+H] + .

Example 59: [4-Chloro-2-methoxy-3-(tetrahydro-2H-pyran-4-yloxy)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using 4-hydroxytetrahydropyran in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. Purification was accomplished by FCC (0-10% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+0.05% TFA) and achiral SFC (Stationary phase: CYANO 6 μM 150×21.2 mm, Mobile phase: 85% CO 2 , 15% MeOH (0.3% iPrNH 2 )). 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.13-8.11 (m, 1H), 7.72 (d, J=8.7 Hz, 1H), 7.39-7.36 (m, 1H), 6.24 (s, 2H), 4.61-4.55 (m, 1H), 4.13 (s, 3H), 4.10-4.06 (m, 2H), 3.97-3.89 (m, 1H), 3.55-3.50 (m, 2H), 3.43 (s, 6H), 2.34 (s, 6H), 2.04-1.93 (m, 4H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 4 , 511.2; m/z found, 512.1 [M+H] + .

Example 60: [4-Chloro-2-methoxy-3-(oxetan-3-ylmethoxy)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using 3-oxetanemethanol in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.15-8.12 (m, 1H), 7.69 (d, J=8.7 Hz, 1H), 7.38-7.33 (m, 1H), 6.19 (s, 2H), 4.94-4.89 (m, 2H), 4.72-4.69 (m, 1H), 4.69-4.65 (m, 2H), 4.40 (d, J=6.9 Hz, 2H), 4.14 (s, 3H), 3.54-3.45 (m, 1H), 3.42 (s, 6H), 2.32 (s, 6H). MS (ESI): mass calcd. for C 25 H 28 ClN 5 O 4 , 497.2; m/z found, 498.1 [M+H] + .

Example 61: {4-Chloro-2-methoxy-3-[(1-methylcyclopropyl)methoxy]quinolin-6-yl}[bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using 1-methylcyclopropanemethanol in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.14-8.12 (m, 1H), 7.67 (d, J=8.7 Hz, 1H), 7.36-7.31 (m, 1H), 6.19 (s, 2H), 4.85 (s, 1H), 4.11 (s, 3H), 3.91 (s, 2H), 3.41 (s, 6H), 2.30 (s, 6H), 1.37 (s, 3H), 0.62-0.59 (m, 2H), 0.48-0.45 (m, 2H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 3 , 495.2; m/z found, 496.2 [M+H] + .

Example 62: {4-Chloro-3-[(3,5-dimethylisoxazol-4-yl)methoxy]-2-methoxyquinolin-6-yl}[bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using (3,5-dimethyl-4-isoxazolyl) methanol in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.13 (d, J=2.2 Hz, 1H), 7.69 (d, J=8.7 Hz, 1H), 7.38 (dd, J=8.8, 2.2 Hz, 1H), 6.15 (s, 2H), 5.34 (s, 1H), 4.95 (s, 2H), 4.16 (s, 3H), 3.41 (s, 6H), 2.40-2.38 (m, 6H), 2.29 (s, 6H). MS (ESI): mass calcd. for C 27 H 29 ClN 6 O 4 , 536.2; m/z found, 537.3 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 5 of 15

Example 63: {4-Chloro-2-methoxy-3-[(3-methyloxetan-3-yl)methoxy]quinolin-6-yl}[bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using 3-methyl-3-oxetanemethanol in place of tetrahydrofurfuryl alcohol using the procedure described for Example 34a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.12 (d, J=2.2 Hz, 1H), 7.72 (d, J=8.7 Hz, 1H), 7.37 (dd, J=8.8, 2.2 Hz, 1H), 6.24 (s, 2H), 4.76 (d, J=5.9 Hz, 2H), 4.51 (d, J=6.0 Hz, 2H), 4.22 (s, 2H), 4.14 (s, 3H), 3.97 (s, 1H), 3.43 (s, 6H), 2.35 (s, 6H), 1.55-1.53 (m, 3H). MS (ESI): mass calcd. for C 26 H 30 ClN 5 O 4 , 511.2; m/z found, 512.3 [M+H] + .

Example 64: [4-Chloro-3-(cyclopropylmethoxy)-2-methoxyquinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

The title compound was prepared using cyclopropanemethanol in place of 2,2-difluorocyclopropylmethanol using the procedure described for Example 35. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.09 (d, J=2.2 Hz, 1H), 7.76 (d, J=8.7 Hz, 1H), 7.39 (dd, J=8.7, 2.2 Hz, 1H), 6.29 (s, 2H), 4.13 (s, 3H), 4.00 (d, J=7.2 Hz, 2H), 3.44 (s, 6H), 3.21 (s, 1H), 2.36 (s, 6H), 1.39-1.31 (m, 1H), 0.65-0.60 (m, 2H), 0.38-0.32 (m, 2H). MS (ESI): mass calcd. for C 25 H 28 ClN 5 O 3 , 481.2; m/z found, 482.1 [M+H] + .

Example 65a: {4-Chloro-2-methoxy-3-[(1-methylpiperidin-4-yl)oxy]quinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared using 4-hydroxy-1-methylpiperidine in place of 3-methyl-3-oxetanemethanol using the procedure described for Example 36a. MS (ESI): mass calcd. for C 27 H 31 ClN 6 O 3 , 522.2; m/z found, 523.6 [M+H] + . {4-Chloro-2-methoxy-3-[(1-methylpiperidin-4-yl)oxy]quinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD 5 μM 250×30 mm, Mobile phase: 67% CO 2 , 33% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The second eluting enantiomer was Example 65b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 7.99 (d, J=2.2 Hz, 1H), 7.81 (d, J=8.7 Hz, 1H), 7.31 (dd, J=8.8, 2.2 Hz, 1H), 6.99 (s, 1H), 6.98-6.93 (m, 2H), 4.47-4.40 (m, 1H), 4.13 (s, 3H), 3.94 (s, 3H), 3.31 (s, 1H), 2.84-2.76 (m, 2H), 2.56 (s, 3H), 2.40 (s, 3H), 2.30 (s, 3H), 2.25-2.16 (m, 2H), 2.01-1.95 (m, 4H). MS (ESI): mass calcd. for C 27 H 31 ClN 6 O 3 , 522.2; m/z found, 523.2 [M+H] + .

Example 66a: [4-Chloro-2-methoxy-3-(pyrimidin-2-ylmethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared using 2-pyrimidinemethanol in place of 3-methyl-3-oxetanemethanol using the procedure described for Example 36a. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.81 (d, J=4.9 Hz, 2H), 8.01-7.98 (m, 1H), 7.83 (d, J=8.7 Hz, 1H), 7.35-7.32 (m, 1H), 7.31-7.29 (m, 1H), 7.00 (s, 1H), 6.96-6.94 (m, 2H), 5.39 (s, 2H), 4.11 (s, 3H), 3.94 (s, 3H), 3.26 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H). MS (ESI): mass calcd. for C 26 H 24 ClN 7 O 3 , 517.2; m/z found, 518.5 [M+H] + . [4-Chloro-2-methoxy-3-(pyrimidin-2-ylmethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD 5 μM 250×30 mm, Mobile phase: 70% CO 2 , 30% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The second eluting enantiomer was Example 66b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.80 (d, J=4.9 Hz, 2H), 8.00-7.98 (m, 1H), 7.83 (d, J=8.7 Hz, 1H), 7.35-7.31 (m, 1H), 7.30-7.27 (m, 1H), 7.00 (s, 1H), 6.97-6.93 (m, 2H), 5.39 (s, 2H), 4.10 (s, 3H), 3.94 (s, 3H), 3.19 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H). MS (ESI): mass calcd. for C 26 H 24 ClN 7 O 3 , 517.2; m/z found, 518.1 [M+H] + .

Example 67a: [4-Chloro-2-methoxy-3-(2-pyrrolidin-1-ylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared using 1-(2-hydroxyethyl)-pyrrolidine in place of 3-methyl-3-oxetanemethanol using the procedure described for Example 36a. MS (ESI): mass calcd. for C 27 H 31 ClN 6 O 3 , 522.2; m/z found, 523.6 [M+H] + . [4-Chloro-2-methoxy-3-(2-pyrrolidin-1-ylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The second eluting enantiomer was Example 67b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 7.98-7.97 (m, 1H), 7.81 (d, J=8.7 Hz, 1H), 7.34-7.30 (m, 1H), 7.00 (s, 1H), 6.97-6.92 (m, 2H), 4.27-4.23 (m, 2H), 4.14 (s, 3H), 3.94 (s, 3H), 3.18 (s, 1H), 2.99-2.94 (m, 2H), 2.68-2.61 (m, 4H), 2.56 (s, 3H), 2.40 (s, 3H), 1.83-1.78 (m, 4H). MS (ESI): mass calcd. for C 27 H 31 ClN 6 O 3 , 522.2; m/z found, 523.0 [M+H] + .

Example 68a: [4-Chloro-3-(cyclopropylmethoxy)-2-methoxyquinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared using cyclopropanemethanol in place of 3-methyl-3-oxetanemethanol using the procedure described for Example 36a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.02 (d, J=2.2 Hz, 1H), 7.77 (d, J=8.7 Hz, 1H), 7.32-7.28 (m, 1H), 6.97-6.91 (m, 2H), 6.86 (s, 1H), 4.64 (s, 1H), 4.13 (s, 3H), 3.98 (d, J=7.3 Hz, 2H), 3.91 (s, 3H), 2.51 (s, 3H), 2.34 (s, 3H), 1.38-1.28 (m, 1H), 0.65-0.59 (m, 2H), 0.36-0.31 (m, 2H). MS (ESI): mass calcd. for C 25 H 26 ClN 5 O 3 , 479.2; m/z found, 480.1 [M+H] + . [4-Chloro-3-(cyclopropylmethoxy)-2-methoxyquinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 68b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 7.99-7.98 (m, 1H), 7.81 (d, J=8.7 Hz, 1H), 7.34-7.30 (m, 1H), 6.99 (s, 1H), 6.98-6.92 (m, 2H), 4.14 (s, 3H), 4.00 (d, J=7.3 Hz, 2H), 3.94 (s, 3H), 3.21 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 1.37-1.28 (m, 1H), 0.65-0.59 (m, 2H), 0.36-0.31 (m, 2H). MS (ESI): mass calcd. for C 25 H 26 ClN 5 O 3 , 479.2; m/z found, 480.1 [M+H] + and the second eluting enantiomer was Example 68c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.00-7.97 (m, 1H), 7.82 (d, J=8.8 Hz, 1H), 7.34-7.30 (m, 1H), 7.00 (s, 1H), 6.99-6.92 (m, 2H), 4.16-4.13 (m, 3H), 4.00 (d, J=7.2 Hz, 2H), 3.96-3.93 (m, 3H), 3.16 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 1.37-1.29 (m, 1H), 0.65-0.59 (m, 2H), 0.36-0.31 (m, 2H). MS (ESI): mass calcd. for C 25 H 26 ClN 5 O 3 , 479.2; m/z found, 480.1 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 6 of 15

Example 69a: [4-Chloro-2-methoxy-3-(tetrahydro-2H-pyran-4-yloxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared using 4-hydroxytetrahydropyran in place of 3-methyl-3-oxetanemethanol using the procedure described for Example 36a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.04 (d, J=2.2 Hz, 1H), 7.78 (d, J=8.7 Hz, 1H), 7.32-7.28 (m, 1H), 6.96-6.91 (m, 2H), 6.87 (s, 1H), 4.60 (s, 1H), 4.59-4.53 (m, 1H), 4.13 (s, 3H), 4.08-4.02 (m, 2H), 3.92 (s, 3H), 3.53-3.46 (m, 2H), 2.52 (s, 3H), 2.35 (s, 3H), 2.00-1.90 (m, 4H). MS (ESI): mass calcd. for C 26 H 28 ClN 5 O 4 , 509.2; m/z found, 510.1 [M+H] + . [4-Chloro-2-methoxy-3-(tetrahydro-2H-pyran-4-yloxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 69b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.01-7.98 (m, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.35-7.30 (m, 1H), 7.00-6.98 (m, 1H), 6.98-6.93 (m, 2H), 4.62-4.55 (m, 1H), 4.14 (s, 3H), 4.10-4.03 (m, 2H), 3.94 (s, 3H), 3.55-3.48 (m, 2H), 3.25 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 2.03-1.91 (m, 4H). MS (ESI): mass calcd. for C 26 H 28 ClN 5 O 4 , 509.2; m/z found, 510.1 [M+H] + and the second eluting enantiomer was Example 69c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.00-7.98 (m, 1H), 7.82 (d, J=8.8 Hz, 1H), 7.34-7.31 (m, 1H), 6.99 (s, 1H), 6.96-6.94 (m, 2H), 4.61-4.55 (m, 1H), 4.14 (s, 3H), 4.09-4.04 (m, 2H), 3.94 (s, 3H), 3.55-3.48 (m, 2H), 3.16 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 2.01-1.90 (m, 4H). MS (ESI): mass calcd. for C 26 H 28 ClN 5 O 4 , 509.2; m/z found, 510.1 [M+H] + .

Example 70a: {4-Chloro-2-methoxy-3-[2-(methylsulfonyl)ethoxy]quinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A mixture of 4-chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinolin-3-ol (200 mg, 0.47 mmol, Intermediate 31), 2-(methylsulfonyl)ethanol (68 μL, 0.7 mmol) and PPh 3 (185 mg, 0.7 mmol) in THF (1.88 mL) was cooled to 0° C. Then DIAD (146 μL, 0.7 mmol) was added dropwise and the mixture was warmed to room temperature and stirred for 30 minutes. Additional 2-(methylsulfonyl)ethanol (68 μL, 0.7 mmol), PPh 3 (185 mg, 0.7 mmol) and DIAD (146 μL, 0.7 mmol) were added and stirring continued at room temperature for 2.5 hours. The reaction was then placed into the sonicator, 2-(methylsulfonyl)ethanol (136 μL, 1.4 mmol), PPh 3 (370 mg, 1.4 mmol) and DIAD (292 μL, 1.4 mmol) were added and the mixture sonicated for 20 minutes. Additional 2-(methylsulfonyl)ethanol (136 μL, 1.4 mmol), PPh 3 (370 mg, 1.4 mmol) and DIAD (292 μL, 1.4 mmol) were added and sonication continued for 1 hour. Additional 2-(methylsulfonyl)ethanol (136 μL, 1.4 mmol), PPh 3 (370 mg, 1.4 mmol) and DIAD (292 μL, 1.4 mmol) were added and sonication continued for 1 hour. The reaction was concentrated to dryness and purified twice by FCC (1-10% MeOH/DCM) to afford the title compound as a light yellow amorphous solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.01 (d, J=2.2 Hz, 1H), 7.84 (d, J=8.8 Hz, 1H), 7.39-7.36 (m, 1H), 6.97-6.94 (m, 3H), 4.57-4.54 (m, 2H), 4.17 (s, 3H), 3.95 (s, 3H), 3.53-3.50 (m, 2H), 3.34 (s, 1H), 3.22-3.20 (m, 3H), 2.56 (s, 3H), 2.39 (s, 3H). MS (ESI): mass calcd. for C 24 H 26 ClN 5 O 5 S, 531.1; m/z found, 532.0 [M+H] + . {4-Chloro-2-methoxy-3-[2-(methylsulfonyl)ethoxy]quinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) followed by FCC (0.5% NH 4 OH, 95% DCM, 5% MeOH) to give 2 enantiomers. The second eluting enantiomer was Example 70b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.00 (d, J=2.2 Hz, 1H), 7.85 (d, J=8.8 Hz, 1H), 7.38 (dd, J=8.8, 2.3 Hz, 1H), 6.97 (s, 1H), 6.96-6.94 (m, 2H), 4.57-4.54 (m, 2H), 4.17 (s, 3H), 3.95 (s, 3H), 3.52 (t, J=5.3 Hz, 2H), 3.22-3.20 (m, 3H), 3.17 (s, 1H), 2.57 (s, 3H), 2.40 (s, 3H). MS (ESI): mass calcd. for C 24 H 26 ClN 5 O 5 S, 531.1; m/z found, 532.0 [M+H] + .

Example 71a: {4-Chloro-3-[(3,5-dimethylisoxazol-4-yl)methoxy]-2-methoxyquinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared using (3,5-dimethyl-4-isoxazolyl) methanol in place of 3-methyl-3-oxetanemethanol using the procedure described for Example 36a. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.03 (d, J=2.2 Hz, 1H), 7.79 (d, J=8.7 Hz, 1H), 7.34 (dd, J=8.8, 2.2 Hz, 1H), 6.97-6.92 (m, 2H), 6.86 (s, 1H), 4.94 (s, 2H), 4.69 (s, 1H), 4.16 (s, 3H), 3.92 (s, 3H), 2.52 (s, 3H), 2.37-2.34 (m, 9H). MS (ESI): mass calcd. for C 27 H 27 ClN 6 O 4 , 534.2; m/z found, 535.2 [M+H] + . {4-Chloro-3-[(3,5-dimethylisoxazol-4-yl)methoxy]-2-methoxyquinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 71b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 7.99-7.98 (m, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.37-7.32 (m, 1H), 6.96 (s, 1H), 6.95 (s, 2H), 4.96 (s, 2H), 4.17 (s, 3H), 3.94 (s, 3H), 3.48 (s, 1H), 2.56 (s, 3H), 2.39 (s, 3H), 2.39-2.37 (m, 6H). MS (ESI): mass calcd. for C 27 H 27 ClN 6 O 4 , 534.2; m/z found, 535.0 [M+H] + and the second eluting enantiomer was Example 71c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 7.99 (d, J=2.2 Hz, 1H), 7.83 (d, J=8.8 Hz, 1H), 7.37-7.33 (m, 1H), 6.97 (s, 1H), 6.95 (s, 2H), 4.96 (s, 2H), 4.17 (s, 3H), 3.94 (s, 3H), 3.43 (s, 1H), 2.56 (s, 3H), 2.39 (s, 3H), 2.39-2.37 (m, 6H). MS (ESI): mass calcd. for C 27 H 27 ClN 6 O 4 , 534.2; m/z found, 535.0 [M+H] + .

Example 72a: {4-Chloro-3-[(2,2-difluorocyclopropyl)methoxy]-2-methoxyquinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 7 of 15

The title compound was prepared using 2,2-difluorocyclopropylmethanol in place of 3-methyl-3-oxetanemethanol using the procedure described for Example 36a. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.00 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.8 Hz, 1H), 7.36-7.32 (m, 1H), 6.98 (s, 1H), 6.95 (s, 2H), 4.30-4.18 (m, 2H), 4.15 (s, 3H), 3.94 (s, 3H), 3.30 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 2.21-2.10 (m, 1H), 1.64-1.57 (m, 1H), 1.35-1.26 (m, 1H). MS (ESI): mass calcd. for C 25 H 24 ClF 2 N 5 O 3 , 515.2; m/z found, 516.2 [M+H] + . {4-Chloro-3-[(2,2-difluorocyclopropyl)methoxy]-2-methoxyquinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 80% CO 2 , 20% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 pairs of enantiomers. The first eluting enantiomer pair was Example 72b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.00 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.33 (dd, J=8.7, 2.2 Hz, 1H), 6.96 (s, 1H), 6.95 (s, 2H), 4.29-4.19 (m, 2H), 4.15 (s, 3H), 3.94 (s, 3H), 3.41 (s, 1H), 2.56 (s, 3H), 2.39 (s, 3H), 2.20-2.10 (m, 1H), 1.65-1.59 (m, 1H), 1.35-1.27 (m, 1H). MS (ESI): mass calcd. for C 25 H 24 ClF 2 N 5 O 3 , 515.2; m/z found, 516.0 [M+H] + and the second eluting enantiomer pair was Example 72c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.00 (d, J=2.2 Hz, 1H), 7.83 (d, J=8.7 Hz, 1H), 7.36-7.32 (m, 1H), 6.99 (s, 1H), 6.95 (s, 2H), 4.29-4.18 (m, 2H), 4.15 (s, 3H), 3.95 (s, 3H), 3.23 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H), 2.20-2.09 (m, 1H), 1.63-1.58 (m, 1H), 1.35-1.27 (m, 1H). MS (ESI): mass calcd. for C 25 H 24 ClF 2 N 5 O 3 , 515.2; m/z found, 516.0 [M+H] + .

Example 73a: [4-Chloro-2-methoxy-3-(1,3-thiazol-2-ylmethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared using 1,3-thiazol-2-ylmethanol in place of 3-methyl-3-oxetanemethanol using the procedure described for Example 36a. MS (ESI): mass calcd. for C 25 H 23 ClN 6 O 3 S, 522.1; m/z found, 523.0 [M+H] + . [4-Chloro-2-methoxy-3-(1,3-thiazol-2-ylmethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 73b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.01 (d, J=2.2 Hz, 1H), 7.83 (d, J=8.7 Hz, 1H), 7.79 (d, J=3.2 Hz, 1H), 7.44 (d, J=3.2 Hz, 1H), 7.34 (dd, J=8.8, 2.2 Hz, 1H), 6.97 (s, 1H), 6.95 (s, 2H), 5.48 (s, 2H), 4.16 (s, 3H), 3.94 (s, 3H), 3.41 (s, 1H), 2.56 (s, 3H), 2.39 (s, 3H). MS (ESI): mass calcd. for C 25 H 23 ClN 6 O 3 S, 522.1; m/z found, 523.0 [M+H] + and the second eluting enantiomer was Example 73c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.02 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.79 (d, J=3.3 Hz, 1H), 7.44 (d, J=3.3 Hz, 1H), 7.35-7.31 (m, 1H), 6.95 (s, 3H), 5.48 (s, 2H), 4.16 (s, 3H), 3.94 (s, 3H), 3.66 (s, 1H), 2.55 (s, 3H), 2.39 (s, 3H). MS (ESI): mass calcd. for C 25 H 23 ClN 6 O 3 S, 522.1; m/z found, 523.0 [M+H] + .

Example 74a: {4-Chloro-3-[2-(dimethylamino)ethoxy]-2-methoxyquinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared using N,N-dimethylethanolamine in place of 4-(2-hydroxyethyl)-morpholine using the procedure described for Example 37a. MS (ESI): mass calcd. for C 25 H 29 ClN 6 O 3 , 496.2; m/z found, 497.2 [M+H] + . {4-Chloro-3-[2-(dimethylamino)ethoxy]-2-methoxyquinolin-6-yl}(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) followed by FCC (0.5% NH 4 OH, 95% DCM, 5% MeOH) to give 2 enantiomers. The second eluting enantiomer was Example 74b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.00 (d, J=2.2 Hz, 1H), 7.81 (d, J=8.8 Hz, 1H), 7.34-7.30 (m, 1H), 6.98 (s, 1H), 6.96-6.94 (m, 2H), 4.23-4.18 (m, 2H), 4.14 (s, 3H), 3.94 (s, 3H), 3.38 (s, 1H), 2.82-2.77 (m, 2H), 2.56 (s, 3H), 2.40 (s, 3H), 2.38 (s, 6H). MS (ESI): mass calcd. for C 25 H 29 ClN 6 O 3 , 496.2; m/z found, 497.1 [M+H] + .

Example 75: [3-(Benzyloxy)-4-chloro-2-methoxyquinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

n-BuLi (1.23 M in hexanes, 18.9 mL, 23.2 mmol) was added dropwise to a stirred slurry of 5-bromo-1,2-dimethyl-1H-imidazole (5.13 g, 27.8 mmol) in THF (40 mL) at −78° C. under nitrogen. After stirring for 20 minutes, the slurry was treated dropwise over 2 minutes with a solution of methyl 3-(benzyloxy)-4-chloro-2-methoxyquinoline-6-carboxylate (3.32 g, 9.28 mmol, Intermediate 32) in THF (20 mL). The flask was then rinsed with THF (10 mL), and that was added to the imidazole flask. The reaction was stirred in the dry ice/acetone bath for 10 minutes, then removed from the cold bath and stirred for 20 minutes, then stirred in an ice bath for 30 minutes. The reaction was then quenched with saturated aqueous NH 4 Cl and concentrated to remove the THF. The aqueous residue was partitioned between water (300 mL) and DCM (250 mL). The layers were separated and the aqueous layer further extracted with DCM (250 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to provide an orange oil. The crude material was purified by FCC (0-10% MeOH/DCM) to afford the title compound as a yellow solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.13 (s, 1H), 7.70 (d, J=8.7 Hz, 1H), 7.58-7.55 (m, 2H), 7.42-7.34 (m, 4H), 6.20 (s, 2H), 5.20 (s, 2H), 4.62 (s, 1H), 4.16 (s, 3H), 3.42 (s, 6H), 2.32 (s, 6H). MS (ESI): mass calcd. for C 28 H 28 ClN 5 O 3 , 517.2; m/z found, 518.1 [M+H] + .

Example 76a: [4-Chloro-2-methoxy-3-(pyridin-3-ylmethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

The title compound was prepared using 3-pyridinemethanol in place of 3-methyl-3-oxetanemethanol and 4-chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methyl)-2-methoxyquinolin-3-ol (Intermediate 30) in place of 4-chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinolin-3-ol using the procedure described for Example 36a. MS (ESI): mass calcd. for C 27 H 21 ClF 3 N 5 O 3 , 555.1; m/z found, 556.0 [M+H] + . [4-Chloro-2-methoxy-3-(pyridin-3-ylmethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 76b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.83-8.81 (m, 1H), 8.73-8.71 (m, 1H), 8.61-8.59 (m, 1H), 8.06-8.04 (m, 1H), 7.93-7.90 (m, 2H), 7.85-7.82 (m, 1H), 7.70-7.67 (m, 1H), 7.53-7.49 (m, 2H), 7.36-7.33 (m, 1H), 6.52-6.51 (m, 1H), 5.20 (s, 2H), 4.16 (s, 3H), 3.42 (s, 3H), 3.29-3.27 (m, 1H). MS (ESI): mass calcd. for C 27 H 21 ClF 3 N 5 O 3 , 555.1; m/z found, 556.5 [M+H] + and the second eluting enantiomer was Example 76c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.83-8.81 (m, 1H), 8.73-8.71 (m, 1H), 8.61-8.59 (m, 1H), 8.06-8.04 (m, 1H), 7.93-7.89 (m, 2H), 7.85-7.81 (m, 1H), 7.71-7.67 (m, 1H), 7.54-7.49 (m, 2H), 7.36-7.33 (m, 1H), 6.53-6.51 (m, 1H), 5.20 (s, 2H), 4.16 (s, 3H), 3.42 (s, 3H), 3.26-3.24 (m, 1H). MS (ESI): mass calcd. for C 27 H 21 ClF 3 N 5 O 3 , 555.1; m/z found, 556.5 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 8 of 15

Example 77a: [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared using [2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA (Example 41) in place of [2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA using the procedure described for Example 38a. MS (ESI): mass calcd. for C 24 H 28 ClN 7 O 2 , 481.2; m/z found, 482.1 [M+H] + . [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 60% CO 2 , 40% iPrOH+(0.3% iPrNH 2 )) followed by FCC (0.5% NH 4 OH, 95% DCM, 5% MeOH) to give 2 enantiomers. The second eluting enantiomer was Example 77b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.01-7.97 (m, 1H), 7.65 (d, J=8.7 Hz, 1H), 7.31-7.27 (m, 1H), 7.10 (s, 1H), 6.15 (s, 1H), 4.70-4.63 (m, 1H), 4.32-4.27 (m, 4H), 3.91 (s, 3H), 3.35 (s, 3H), 2.42-2.34 (m, 2H), 2.22 (s, 3H), 1.35 (d, J=6.1 Hz, 6H). MS (ESI): mass calcd. for C 24 H 28 ClN 7 O 2 , 481.2; m/z found, 482.1 [M+H] + .

Example 78a: {4-Chloro-2-methoxy-3-[(3-methyloxetan-3-yl)methoxy]quinolin-6-yl}(1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

The title compound was prepared using 4-chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methyl)-2-methoxyquinolin-3-ol (Intermediate 30) in place of 4-chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinolin-3-ol using the procedure described for Example 36a. MS (ESI): mass calcd. for C 26 H 24 ClF 3 N 4 O 4 , 548.1; m/z found, 549.0 [M+H] + . {4-Chloro-2-methoxy-3-[(3-methyloxetan-3-yl)methoxy]quinolin-6-yl}(1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified by chiral SFC (Stationary phase: CHIRALCEL OJ-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% MeOH+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 78b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.83-8.79 (m, 1H), 8.08 (d, J=2.1 Hz, 1H), 7.93-7.89 (m, 1H), 7.82-7.79 (m, 1H), 7.68-7.65 (m, 1H), 7.48 (dd, J=8.7, 2.2 Hz, 1H), 7.37 (s, 1H), 6.39-6.36 (m, 1H), 4.75-4.72 (m, 2H), 4.71-4.64 (m, 1H), 4.51-4.48 (m, 2H), 4.17 (s, 2H), 4.13 (s, 3H), 3.37 (s, 3H), 1.54-1.51 (m, 3H). MS (ESI): mass calcd. for C 26 H 24 ClF 3 N 4 O 4 , 548.1; m/z found, 549.2 [M+H] + and the second eluting enantiomer was Example 78c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.82-8.79 (m, 1H), 8.08 (d, J=2.1 Hz, 1H), 7.93-7.88 (m, 1H), 7.80 (d, J=8.7 Hz, 1H), 7.68-7.64 (m, 1H), 7.50-7.46 (m, 1H), 7.35 (s, 1H), 6.38-6.34 (m, 1H), 4.94 (s, 1H), 4.75-4.71 (m, 2H), 4.50-4.47 (m, 2H), 4.17 (s, 2H), 4.13 (s, 3H), 3.37 (s, 3H), 1.53-1.50 (m, 3H). MS (ESI): mass calcd. for C 26 H 24 ClF 3 N 4 O 4 , 548.1; m/z found, 549.2 [M+H] + .

Example 79a: [4-Chloro-2-methoxy-3-(2-methoxyethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

The title compound was prepared using 2-methoxyethanol in place of 4-(2-hydroxyethyl)-morpholine and 4-chloro-6-(hydroxy(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methyl)-2-methoxyquinolin-3-ol (Intermediate 30) in place of 4-chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinolin-3-ol using the procedure described for Example 37a. MS (ESI): mass calcd. for C 24 H 22 ClF 3 N 4 O 4 , 522.1; m/z found, 523.0 [M+H] + . [4-Chloro-2-methoxy-3-(2-methoxyethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 80% CO 2 , 20% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 79b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.84-8.80 (m, 1H), 8.06 (d, J=2.1 Hz, 1H), 7.93-7.89 (m, 1H), 7.81 (d, J=8.8 Hz, 1H), 7.67 (d, J=7.9 Hz, 1H), 7.51-7.45 (m, 2H), 6.47 (s, 1H), 4.30-4.27 (m, 2H), 4.13 (s, 3H), 3.80-3.76 (m, 2H), 3.45 (s, 3H), 3.40 (s, 3H). MS (ESI): mass calcd. for C 24 H 22 ClF 3 N 4 O 4 , 522.1; m/z found, 523.0 [M+H] + and the second eluting enantiomer was Example 79c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.83-8.80 (m, 1H), 8.06 (d, J=2.1 Hz, 1H), 7.94-7.89 (m, 1H), 7.80 (d, J=8.7 Hz, 1H), 7.67 (d, J=8.2 Hz, 1H), 7.50-7.46 (m, 1H), 7.43 (s, 1H), 6.44 (s, 1H), 4.31-4.26 (m, 2H), 4.13 (s, 3H), 3.80-3.75 (m, 2H), 3.45 (s, 3H), 3.39 (s, 3H). MS (ESI): mass calcd. for C 24 H 22 ClF 3 N 4 O 4 , 522.1; m/z found, 523.0 [M+H] + .

Example 80a: [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

n-BuLi (1.23 M in hexanes, 1.1 mL, 1.35 mmol) was added dropwise to a stirred slurry of 1-methyl-1,2,3-triazole (112 mg, 1.35 mmol) in THF (1 mL) at −40° C. under nitrogen. After stirring for another 30 minutes, the mixture was treated dropwise with a solution of (4-chloro-3-isopropoxy-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (252 mg, 0.67 mmol, Intermediate 27) in THF (5 mL). The reaction was allowed to warm to room temperature over 1 hour. The reaction was then quenched with saturated aqueous NH 4 Cl. The mixture was poured into a separatory funnel and extracted with DCM (4×60 mL). The organics were combined, washed with brine, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a light yellow oil. The crude material was purified by FCC (1-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to provide the title compound as a white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.08 (d, J=2.2 Hz, 1H), 7.78 (d, J=8.7 Hz, 1H), 7.33 (dd, J=8.6, 2.2 Hz, 1H), 7.18 (s, 1H), 6.18 (s, 1H), 4.74-4.67 (m, 1H), 4.32 (s, 1H), 4.13 (s, 3H), 3.94 (s, 3H), 3.39 (s, 3H), 2.32 (s, 3H), 1.39 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 22 H 25 ClN 6 O 3 , 456.2; m/z found, 457.0 [M+H] + . [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% MeOH/iPrOH 50/50 v/v+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 80b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.09 (d, J=2.2 Hz, 1H), 7.77 (d, J=8.7 Hz, 1H), 7.32 (dd, J=8.7, 2.2 Hz, 1H), 7.17 (s, 1H), 6.17 (s, 1H), 4.75-4.66 (m, 1H), 4.36 (s, 1H), 4.13 (s, 3H), 3.94 (s, 3H), 3.39 (s, 3H), 2.32 (s, 3H), 1.39 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 22 H 25 ClN 6 O 3 , 456.2; m/z found, 457.2 [M+H] + and the second eluting enantiomer was Example 80c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.06 (d, J=2.2 Hz, 1H), 7.80 (d, J=8.7 Hz, 1H), 7.35-7.32 (m, 1H), 7.21 (s, 1H), 6.24 (s, 1H), 4.74-4.67 (m, 1H), 4.13 (s, 3H), 3.95 (s, 3H), 3.52 (s, 1H), 3.42 (s, 3H), 2.37 (s, 3H), 1.39 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 22 H 25 ClN 6 O 3 , 456.2; m/z found, 457.2 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 9 of 15

Example 81a: [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

To a mixture of [2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA (182 mg, 0.31 mmol, Example 42) in toluene (3.1 mL) was added NaOMe (168 mg, 3.1 mmol) and the resulting mixture heated to 60° C. for 3 hours. The mixture was then cooled to room temperature, diluted with DCM and filtered through a pad of Celite®, rinsing the filter cake with DCM. The filtrate was concentrated to dryness and the residue purified by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to provide the title compound as a white solid. MS (ESI): mass calcd. for C 24 H 26 ClN 5 O 3 , 467.2; m/z found, 468.0 [M+H] + . [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% iPrOH+(0.3% iPrNH 2 )) to give 2 enantiomers. The first eluting enantiomer was Example 81b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.00-7.98 (m, 1H), 7.83-7.79 (m, 1H), 7.31 (d, J=8.7 Hz, 1H), 7.00-6.99 (m, 1H), 6.98-6.93 (m, 2H), 4.73-4.67 (m, 1H), 4.14-4.12 (m, 3H), 3.95-3.93 (m, 3H), 3.23 (s, 1H), 2.57-2.54 (m, 3H), 2.41-2.39 (m, 3H), 1.40-1.37 (m, 6H). MS (ESI): mass calcd. for C 24 H 26 ClN 5 O 3 , 467.2; m/z found, 468.1 [M+H] + and the second eluting enantiomer was Example 81c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.00-7.98 (m, 1H), 7.83-7.80 (m, 1H), 7.32-7.29 (m, 1H), 7.00-6.99 (m, 1H), 6.98-6.93 (m, 2H), 4.73-4.67 (m, 1H), 4.14-4.13 (m, 3H), 3.95-3.93 (m, 3H), 3.25-3.23 (m, 1H), 2.57-2.55 (m, 3H), 2.41-2.39 (m, 3H), 1.40-1.37 (m, 6H). MS (ESI): mass calcd. for C 24 H 26 ClN 5 O 3 , 467.2; m/z found, 468.0 [M+H] + .

Example 82a: [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](2,4-dimethyl-1,3-thiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA

To a sealed tube was added [2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](2,4-dimethyl-1,3-thiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA (185 mg, 0.31 mmol, Example 43), azetidine (107 μL, 1.56 mmol) and dimethylformamide (1.6 mL). The reaction vessel was sealed and heated in a 100° C. oil bath. After overnight heating, the vessel was cooled and the contents concentrated to dryness. The residue was dissolved in EtOAc (25 mL) and washed with saturated aqueous ammonium chloride (2×20 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a light yellow oil. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.05 (d, J=8.9 Hz, 1H), 7.86 (d, J=2.1 Hz, 1H), 7.75-7.72 (m, 1H), 7.13 (s, 1H), 4.93-4.86 (m, 1H), 4.78-4.73 (m, 4H), 3.94 (s, 3H), 2.65 (s, 3H), 2.61-2.55 (m, 2H), 2.09 (s, 3H), 1.42-1.39 (m, 6H). MS (ESI): mass calcd. for C 24 H 27 ClN 6 O 2 S, 498.2; m/z found, 499.0 [M+H] + . [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](2,4-dimethyl-1,3-thiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: Kromasil 5-Amycoat 5 μM 250×30 mm, Mobile phase: 85% CO 2 , 15% EtOH+(0.2% Et 3 N)) to give 2 enantiomers. The first eluting enantiomer was Example 82b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 7.92-7.90 (m, 1H), 7.69 (d, J=8.8 Hz, 1H), 7.38-7.34 (m, 1H), 7.22 (s, 1H), 4.70-4.64 (m, 1H), 4.32-4.28 (m, 4H), 3.92 (s, 3H), 2.59 (s, 3H), 2.41-2.34 (m, 2H), 2.12 (s, 3H), 1.34 (d, J=6.1 Hz, 6H). MS (ESI): mass calcd. for C 24 H 27 ClN 6 O 2 S, 498.2; m/z found, 499.0 [M+H] + and the second eluting enantiomer was Example 82c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 7.92-7.90 (m, 1H), 7.71-7.67 (m, 1H), 7.38-7.34 (m, 1H), 7.24-7.22 (m, 1H), 4.70-4.64 (m, 1H), 4.32-4.28 (m, 4H), 3.92 (s, 3H), 2.59 (s, 3H), 2.41-2.34 (m, 2H), 2.13 (s, 3H), 1.36-1.33 (m, 6H). MS (ESI): mass calcd. for C 24 H 27 ClN 6 O 2 S, 498.2; m/z found, 499.0 [M+H] + .

Example 83: (4-Chloro-2-methoxy-3-{[4-(trifluoromethyl)piperidin-1-yl]carbonyl}quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

n-BuLi (1.85 M in hexanes, 116 μL, 0.21 mmol) was added dropwise to a stirred solution of (4-chloro-6-iodo-2-methoxyquinolin-3-yl)(4-(trifluoromethyl)piperidin-1-yl)methanone (107 mg, 0.21 mmol, Intermediate 35: step f) in THF (3 mL) at −78° C. under nitrogen. After stirring for 5 minutes at −78° C., the mixture was treated dropwise with a solution of (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (46 mg, 0.21 mmol, Intermediate 11: step b) in THF (2 mL). The flask was rinsed with THF (1 mL) and that THF was added to the reaction. The solution was stirred at −78° C. for 15 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (5 mL), water (20 mL) and EtOAc (20 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (20 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow oil. The crude material was purified by FCC (0.5-7.5% MeOH/DCM) to provide the title compound as a cream-colored amorphous solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.15-8.01 (m, 1H), 7.91-7.85 (m, 1H), 7.54-7.39 (m, 1H), 7.06-6.98 (m, 1H), 6.97-6.90 (m, 2H), 4.98-4.91 (m, 1H), 4.13-4.09 (m, 3H), 3.97-3.93 (m, 3H), 3.58-3.52 (m, 1H), 3.22-3.20 (m, 1H), 3.16-3.07 (m, 1H), 2.90-2.82 (m, 1H), 2.58-2.55 (m, 3H), 2.42-2.38 (m, 3H), 2.37-2.28 (m, 1H), 2.09-2.01 (m, 1H), 1.89-1.80 (m, 1H), 1.74-1.58 (m, 2H). MS (ESI): mass calcd. for C 28 H 28 ClF 3 N 6 O 3 , 588.2; m/z found, 589.2 [M+H] + .

Example 84a: (1-Acetylpiperidin-4-yl)[4-chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol

To a mixture of (1-acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA (179 mg, 0.3 mmol, Example 44) in toluene (3 mL) was added NaOMe (161 mg, 2.98 mmol) and the resulting mixture heated to 60° C. for 17 hours. The mixture was then cooled to room temperature, diluted with DCM and filtered, rinsing the filter cake with DCM. The filtrate was concentrated to dryness to afford a white solid. The crude material was purified by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to provide the title compound as a white solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.21-8.18 (m, 1H), 7.76-7.71 (m, 1H), 7.63-7.57 (m, 1H), 7.54-7.50 (m, 2H), 7.36-7.30 (m, 2H), 7.24-7.21 (m, 1H), 4.74-4.68 (m, 1H), 4.68-4.64 (m, 1H), 4.09 (s, 3H), 3.88-3.79 (m, 1H), 3.14-3.04 (m, 1H), 2.80-2.72 (m, 1H), 2.63-2.53 (m, 1H), 2.20-2.18 (m, 1H), 2.06-2.04 (m, 3H), 1.70-1.57 (m, 1H), 1.51-1.41 (m, 1H), 1.38-1.36 (m, 6H), 1.35-1.24 (m, 1H). MS (ESI): mass calcd. for C 27 H 31 ClN 2 O 4 , 482.2; m/z found, 483.1 [M+H] + . (1-Acetylpiperidin-4-yl)[4-chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% MeOH/iPrOH 50/50 v/v) to give 2 enantiomers. The first eluting enantiomer was Example 84b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.20 (d, J=8.7 Hz, 1H), 7.75-7.71 (m, 1H), 7.63-7.57 (m, 1H), 7.55-7.50 (m, 2H), 7.36-7.30 (m, 2H), 7.24-7.20 (m, 1H), 4.74-4.68 (m, 1H), 4.68-4.63 (m, 1H), 4.10-4.08 (m, 3H), 3.87-3.78 (m, 1H), 3.13-3.04 (m, 1H), 2.80-2.72 (m, 1H), 2.63-2.53 (m, 1H), 2.21 (s, 1H), 2.07-2.04 (m, 3H), 1.73-1.56 (m, 2H), 1.51-1.41 (m, 1H), 1.37 (d, J=6.3 Hz, 6H), 1.34-1.29 (m, 1H). MS (ESI): mass calcd. for C 27 H 31 ClN 2 O 4 , 482.2; m/z found, 483.1 [M+H] + and the second eluting enantiomer was Example 84c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.21-8.18 (m, 1H), 7.73 (t, J=8.6 Hz, 1H), 7.63-7.57 (m, 1H), 7.55-7.50 (m, 2H), 7.36-7.30 (m, 2H), 7.25-7.19 (m, 1H), 4.74-4.68 (m, 1H), 4.68-4.63 (m, 1H), 4.09 (s, 3H), 3.87-3.78 (m, 1H), 3.14-3.03 (m, 1H), 2.80-2.72 (m, 1H), 2.63-2.53 (m, 1H), 2.24-2.22 (m, 1H), 2.06-2.04 (m, 3H), 1.71-1.56 (m, 2H), 1.51-1.41 (m, 1H), 1.38-1.36 (m, 6H), 1.36-1.30 (m, 1H). MS (ESI): mass calcd. for C 27 H 31 ClN 2 O 4 , 482.2; m/z found, 483.1 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 10 of 15

Example 85a: [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](2,4-dimethyl-1,3-thiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

To a mixture of [2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](2,4-dimethyl-1,3-thiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol.TFA (139 mg, 0.23 mmol, Example 43) in toluene (2.4 mL) was added NaOMe (127 mg, 2.4 mmol) and the resulting mixture heated to 60° C. for 5.5 hours. The mixture was then cooled to room temperature, diluted with DCM and filtered, rinsing the filter cake with DCM. The filtrate was concentrated to dryness to afford a white solid. The crude material was purified by basic HPLC to provide the title compound as a white solid. MS (ESI): mass calcd. for C 22 H 24 ClN 5 O 3 S, 473.1; m/z found, 474.0 [M+H] + . [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](2,4-dimethyl-1,3-thiazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 80% CO 2 , 20% MeOH) followed by achiral SFC (Stationary phase: CHIRALPAK IC 5 μM 250×20 mm, Mobile phase: 60% CO 2 , 40% MeOH) to give 2 enantiomers. The first eluting enantiomer was Example 85b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.08-8.06 (m, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.46-7.43 (m, 1H), 7.23 (s, 1H), 4.73-4.66 (m, 1H), 4.13 (s, 3H), 3.92 (s, 3H), 3.65-3.63 (m, 1H), 2.59 (s, 3H), 2.15 (s, 3H), 1.38 (d, J=6.0 Hz, 6H). MS (ESI): mass calcd. for C 22 H 24 ClN 5 O 3 S, 473.1; m/z found, 474.0 [M+H] + and the second eluting enantiomer was Example 85c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.07 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.46-7.43 (m, 1H), 7.23 (s, 1H), 4.73-4.67 (m, 1H), 4.13 (s, 3H), 3.92 (s, 3H), 3.65 (s, 1H), 2.59 (s, 3H), 2.15 (s, 3H), 1.38 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 22 H 24 ClN 5 O 3 S, 473.1; m/z found, 474.0 [M+H] + .

Example 86a: [4-Chloro-2-methoxy-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol

To a mixture of (4-chlorophenyl)[2,4-dichloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)methanol (133 mg, 0.26 mmol, Example 45) in toluene (2.6 mL) was added NaOMe (97 mg, 1.8 mmol) and the resulting mixture heated to 60° C. for 6 hours. The mixture was then cooled to room temperature, diluted with DCM and filtered, rinsing the filter cake with DCM. The filtrate was concentrated to dryness and the residue purified by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to provide the title compound as a white solid. MS (ESI): mass calcd. for C 23 H 18 Cl 2 F 3 N 3 O 3 , 511.1; m/z found, 512.9 [M+H] + . [4-Chloro-2-methoxy-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol was purified by achiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 80% CO 2 , 20% MeOH) followed by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 75% CO 2 , 25% MeOH/iPrOH 50/50 v/v) to give 2 enantiomers. The first eluting enantiomer was Example 86b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.08 (d, J=2.1 Hz, 1H), 7.77 (d, J=8.8 Hz, 1H), 7.53-7.48 (m, 1H), 7.35-7.28 (m, 5H), 6.37-6.34 (m, 1H), 4.51 (q, J=8.4 Hz, 2H), 4.15 (s, 3H), 4.13-4.05 (m, 1H), 3.37 (s, 3H). MS (ESI): mass calcd. for C 23 H 18 Cl 2 F 3 N 3 O 3 , 511.1; m/z found, 512.0 [M+H] + and the second eluting enantiomer was Example 86c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.08 (d, J=2.1 Hz, 1H), 7.77 (d, J=8.8 Hz, 1H), 7.53-7.49 (m, 1H), 7.36-7.28 (m, 5H), 6.37-6.34 (m, 1H), 4.55-4.47 (m, 2H), 4.15 (s, 3H), 4.08 (s, 1H), 3.37 (s, 3H). MS (ESI): mass calcd. for C 23 H 18 Cl 2 F 3 N 3 O 3 , 511.1; m/z found, 512.0 [M+H] + .

Example 87a: [2-Azetidin-1-yl-4-chloro-3-(cyclopropylmethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a sealed tube was added [2,4-dichloro-3-(cyclopropylmethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA (112 mg, 0.18 mmol, Example 40), azetidine (61 μL, 0.88 mmol) and dimethylformamide (0.92 mL). The reaction vessel was sealed and heated in a 100° C. oil bath. After overnight heating, the vessel was cooled and the contents concentrated to dryness. The residue was dissolved in EtOAc (15 mL) and washed with saturated aqueous ammonium chloride (2×15 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a light yellow oil. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. MS (ESI): mass calcd. for C 27 H 25 ClF 3 N 5 O 2 , 543.2; m/z found, 544.0 [M+H] + . [2-Azetidin-1-yl-4-chloro-3-(cyclopropylmethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×21 mm, Mobile phase: 80% CO 2 , 20% EtOH+0.2% Et 3 N) to give 2 enantiomers. The first eluting enantiomer was Example 87b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.81 (s, 1H), 7.93-7.85 (m, 2H), 7.68-7.61 (m, 2H), 7.41-7.34 (m, 1H), 7.34-7.28 (m, 1H), 6.39-6.28 (m, 1H), 4.35-4.30 (m, 4H), 3.82 (d, J=7.2 Hz, 2H), 3.36 (s, 3H), 2.43-2.35 (m, 2H), 1.35-1.30 (m, 1H), 0.68-0.62 (m, 2H), 0.38-0.32 (m, 2H). MS (ESI): mass calcd. for C 27 H 25 ClF 3 N 5 O 2 , 543.2; m/z found, 544.0 [M+H] + and the second eluting enantiomer was Example 87c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.81 (s, 1H), 7.92-7.85 (m, 2H), 7.68-7.62 (m, 2H), 7.40-7.34 (m, 1H), 7.34-7.29 (m, 1H), 6.39-6.28 (m, 1H), 4.35-4.29 (m, 4H), 3.82 (d, J=7.1 Hz, 2H), 3.36 (s, 3H), 2.43-2.34 (m, 2H), 1.34-1.29 (m, 1H), 0.67-0.61 (m, 2H), 0.37-0.32 (m, 2H). MS (ESI): mass calcd. for C 27 H 25 ClF 3 N 5 O 2 , 543.2; m/z found, 544.0 [M+H] + .

Example 88a: [4-Chloro-3-(cyclopropylmethoxy)-2-methoxyquinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a mixture of [2,4-dichloro-3-(cyclopropylmethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA (112 mg, 0.21 mmol, Example 40) in toluene (2.1 mL) was added NaOMe (115 mg, 2.1 mmol) and the resulting mixture heated to 60° C. for 6 hours. The mixture was then cooled to room temperature, diluted with DCM and filtered, rinsing the filter cake with DCM. The filtrate was concentrated to dryness to afford a clear colorless oil. The crude material was purified by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to provide a white solid. MS (ESI): mass calcd. for C 25 H 22 ClF 3 N 4 O 3 , 518.1; m/z found, 519.0 [M+H] + . [4-Chloro-3-(cyclopropylmethoxy)-2-methoxyquinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 80% CO 2 , 20% MeOH/iPrOH 50/50 v/v) to give 2 enantiomers. The first eluting enantiomer was Example 88b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.85-8.82 (m, 1H), 8.06 (d, J=2.1 Hz, 1H), 7.91-7.86 (m, 1H), 7.81 (d, J=8.8 Hz, 1H), 7.68-7.64 (m, 1H), 7.52-7.48 (m, 1H), 7.48-7.46 (m, 1H), 6.41-6.37 (m, 1H), 4.12 (s, 3H), 3.98 (d, J=7.3 Hz, 2H), 3.42 (s, 3H), 3.41-3.39 (m, 1H), 1.37-1.28 (m, 1H), 0.64-0.59 (m, 2H), 0.36-0.31 (m, 2H). MS (ESI): mass calcd. for C 25 H 22 ClF 3 N 4 O 3 , 518.1; m/z found, 519.0 [M+H] + and the second eluting enantiomer was Example 88c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.85-8.82 (m, 1H), 8.07-8.04 (m, 1H), 7.90-7.85 (m, 1H), 7.82-7.79 (m, 1H), 7.69-7.64 (m, 1H), 7.52-7.47 (m, 2H), 6.39 (s, 1H), 4.14-4.11 (m, 3H), 4.00-3.96 (m, 2H), 3.44-3.41 (m, 3H), 3.41-3.38 (m, 1H), 1.37-1.28 (m, 1H), 0.65-0.58 (m, 2H), 0.36-0.30 (m, 2H). MS (ESI): mass calcd. for C 25 H 22 ClF 3 N 4 O 3 , 518.1; m/z found, 519.0 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 11 of 15

Example 89a: [2-Azetidin-1-yl-4-chloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a sealed tube was added [2,4-dichloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol (86 mg, 0.13 mmol, Example 39), azetidine (54 μL, 0.78 mmol) and dimethylformamide (0.82 mL). The reaction vessel was sealed and heated in a 100° C. oil bath. After overnight heating, the vessel was cooled and the contents concentrated to dryness. The residue was dissolved in EtOAc (15 mL) and washed with saturated aqueous ammonium chloride (2×10 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a light yellow oil. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. MS (ESI): mass calcd. for C 25 H 20 ClF 6 N 5 O 2 , 571.1; m/z found, 572.0 [M+H] + . [2-Azetidin-1-yl-4-chloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×20 mm, Mobile phase: 70% CO 2 , 30% EtOH) to give 2 enantiomers. The first eluting enantiomer was Example 89b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.84-8.80 (m, 1H), 7.96-7.93 (m, 1H), 7.89-7.84 (m, 1H), 7.73-7.68 (m, 1H), 7.68-7.64 (m, 1H), 7.52-7.48 (m, 1H), 7.48-7.44 (m, 1H), 6.39 (s, 1H), 4.39-4.33 (m, 2H), 4.33-4.28 (m, 4H), 3.45-3.42 (m, 3H), 3.42-3.40 (m, 1H), 2.47-2.41 (m, 2H). MS (ESI): mass calcd. for C 25 H 20 ClF 6 N 5 O 2 , 571.1; m/z found, 572.0 [M+H] + and the second eluting enantiomer was Example 89c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.84-8.80 (m, 1H), 7.95-7.92 (m, 1H), 7.89-7.84 (m, 1H), 7.71 (d, J=8.8 Hz, 1H), 7.68-7.64 (m, 1H), 7.50 (s, 1H), 7.48-7.43 (m, 1H), 6.39 (s, 1H), 4.39-4.33 (m, 2H), 4.33-4.28 (m, 4H), 3.42 (s, 3H), 3.42-3.39 (m, 1H), 2.47-2.41 (m, 2H). MS (ESI): mass calcd. for C 25 H 20 ClF 6 N 5 O 2 , 571.1; m/z found, 572.0 [M+H] + .

Example 90a: [2-Azetidin-1-yl-4-chloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol.TFA

To a sealed tube was added (4-chlorophenyl)[2,4-dichloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)methanol (133 mg, 0.26 mmol, Example 45), azetidine (89 μL, 1.29 mmol) and dimethylformamide (1.35 mL). The reaction vessel was sealed and heated in a 100° C. oil bath. After overnight heating, the vessel was cooled and the contents concentrated to dryness. The residue was dissolved in EtOAc (15 mL) and washed with saturated aqueous ammonium chloride (2×10 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a light yellow oil. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.66 (s, 1H), 7.93-7.91 (m, 1H), 7.87-7.82 (m, 1H), 7.57-7.52 (m, 1H), 7.36-7.32 (m, 2H), 7.26-7.25 (m, 1H), 7.25-7.23 (m, 1H), 6.67-6.63 (m, 1H), 4.60-4.54 (m, 4H), 4.48-4.41 (m, 2H), 3.61-3.58 (m, 3H), 2.54-2.47 (m, 2H). MS (ESI): mass calcd. for C 25 H 21 Cl 2 F 3 N 4 O 2 , 536.1; m/z found, 537.0 [M+H] + . [2-Azetidin-1-yl-4-chloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol was purified by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 μM 250×21 mm, Mobile phase: 85% CO 2 , 15% EtOH+0.2% Et 3 N) to give 2 enantiomers. The first eluting enantiomer was Example 90b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 7.94-7.90 (m, 1H), 7.68-7.64 (m, 1H), 7.45-7.40 (m, 1H), 7.39-7.34 (m, 1H), 7.33-7.28 (m, 4H), 6.44-6.32 (m, 1H), 4.36-4.31 (m, 2H), 4.31-4.27 (m, 4H), 3.38 (s, 3H), 2.44-2.37 (m, 2H). MS (ESI): mass calcd. for C 25 H 21 Cl 2 F 3 N 4 O 2 , 536.1; m/z found, 537.0 [M+H] + and the second eluting enantiomer was Example 90c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 7.93-7.90 (m, 1H), 7.66 (d, J=8.7 Hz, 1H), 7.50-7.38 (m, 2H), 7.33-7.27 (m, 4H), 6.50-6.35 (m, 1H), 4.36-4.31 (m, 2H), 4.31-4.27 (m, 4H), 3.38 (s, 3H), 2.44-2.37 (m, 2H). MS (ESI): mass calcd. for C 25 H 21 Cl 2 F 3 N 4 O 2 , 536.1; m/z found, 537.0 [M+H] + .

Example 91a: (4-Chloro-3-ethoxy-2-methoxyquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a mixture of (2,4-dichloro-3-ethoxyquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA (176 mg, 0.35 mmol, Example 46) in toluene (3.5 mL) was added NaOMe (191 mg, 3.5 mmol) and the resulting mixture heated to 60° C. for 2 hours. Then, the temperature was raised to 80° C. and stirring continued for 3 hours. The mixture was then cooled to room temperature, diluted with DCM and filtered, rinsing the filter cake with DCM. The filtrate was concentrated to dryness and the residue purified by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to afford the title compound as a tan solid. MS (ESI): mass calcd. for C 23 H 20 ClF 3 N 4 O 3 , 492.1; m/z found, 493.0 [M+H] + . (4-Chloro-3-ethoxy-2-methoxyquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified by chiral HPLC (Stationary phase: CHIRALPAK AD-H 5 μM Daicel column, Mobile phase: 85/12/3 heptane/ethanol/methanol (with 6 N ammonia)) followed by achiral SFC (Stationary phase: CHIRALPAK IC 5 μM 250×21 mm, Mobile phase: 90% CO 2 , 10% EtOH+0.2% Et 3 N) to give 2 enantiomers. The first eluting enantiomer was Example 91b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.86-8.77 (m, 1H), 8.08 (s, 1H), 7.94-7.85 (m, 1H), 7.81-7.75 (m, 1H), 7.68-7.62 (m, 1H), 7.51-7.43 (m, 1H), 7.27-7.26 (m, 1H), 6.39-6.24 (m, 1H), 4.24-4.16 (m, 2H), 4.12 (s, 3H), 3.36 (s, 3H), 1.48-1.43 (m, 3H). MS (ESI): mass calcd. for C 23 H 20 ClF 3 N 4 O 3 , 492.1; m/z found, 493.0 [M+H] + and the second eluting enantiomer was Example 91c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.83-8.80 (m, 1H), 8.08 (d, J=2.2 Hz, 1H), 7.92-7.88 (m, 1H), 7.78 (d, J=8.7 Hz, 1H), 7.67-7.64 (m, 1H), 7.49-7.45 (m, 1H), 7.31-7.27 (m, 1H), 6.33-6.30 (m, 1H), 5.32 (s, 1H), 4.23-4.17 (m, 2H), 4.12 (s, 3H), 3.35 (s, 3H), 1.47-1.43 (m, 3H). MS (ESI): mass calcd. for C 23 H 20 ClF 3 N 4 O 3 , 492.1; m/z found, 493.0 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 12 of 15

Example 92a: (4-Chloro-3-ethoxy-2-methoxyquinolin-6-yl)(4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol

To a mixture of (4-chlorophenyl)(2,4-dichloro-3-ethoxyquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)methanol.TFA (143 mg, 0.31 mmol, Example 47) in toluene (3.1 mL) was added NaOMe (167 mg, 3.1 mmol) and the resulting mixture heated to 60° C. for 6 hours. The reaction was then heated to 80° C. and stirred for 17.5 hours. The mixture was then cooled to room temperature, diluted with DCM and filtered, rinsing the filter cake with DCM. The filtrate was concentrated to dryness and purified by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to afford the title compound as a white solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.03-8.01 (m, 1H), 7.79-7.76 (d, J=8.7 Hz, 1H), 7.49-7.46 (m, 1H), 7.46-7.44 (m, 1H), 7.34-7.29 (m, 4H), 6.46-6.44 (m, 1H), 4.23-4.18 (m, 2H), 4.14-4.12 (s, 3H), 3.42-3.39 (s, 3H), 3.15-3.12 (s, 1H), 1.47-1.43 (m, 3H). MS (ESI): mass calcd. for C 23 H 21 Cl 2 N 3 O 3 , 457.1; m/z found, 459.0 [M+H] + . (4-Chloro-3-ethoxy-2-methoxyquinolin-6-yl)(4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol was purified by chiral HPLC (Stationary phase: CHIRALPAK AD-H 20 μM Daicel column, Mobile phase: 1/1 MeOH/EtOH) followed by silica plug (5% MeOH/DCM or 50% EtOAc/DCM) to give 2 enantiomers. The first eluting enantiomer was Example 92b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 7.98 (d, J=2.1 Hz, 1H), 7.72 (d, J=8.8 Hz, 1H), 7.47-7.43 (m, 1H), 7.41 (s, 1H), 7.26-7.25 (m, 4H), 6.31 (s, 1H), 4.18-4.11 (m, 2H), 4.07 (s, 3H), 3.37 (s, 3H), 1.42-1.38 (m, 3H). MS (ESI): mass calcd. for C 23 H 21 Cl 2 N 3 O 3 , 457.1; m/z found, 458.0 [M+H] + and the second eluting enantiomer was Example 92c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.04 (d, J=2.1 Hz, 1H), 7.76 (d, J=8.7 Hz, 1H), 7.49-7.44 (m, 1H), 7.36 (s, 1H), 7.33-7.30 (m, 4H), 6.38 (s, 1H), 4.23-4.17 (m, 2H), 4.12 (s, 3H), 3.38 (s, 3H), 1.48-1.42 (m, 3H). MS (ESI): mass calcd. for C 23 H 21 Cl 2 N 3 O 3 , 457.1; m/z found, 458.0 [M+H] + .

Example 93a: [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a sealed tube was added [2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA (151 mg, 0.3 mmol, Example 48), azetidine (100 μL, 1.48 mmol) and dimethylformamide (1.55 mL). The reaction vessel was sealed and heated in a 100° C. oil bath. After overnight heating, the vessel was cooled to room temperature and the contents concentrated to dryness. The residue was dissolved in EtOAc (15 mL) and washed with saturated aqueous ammonium chloride (2×10 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a light yellow foam. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. MS (ESI): mass calcd. for C 26 H 25 ClF 3 N 5 O 2 , 531.2; m/z found, 532.0 [M+H] + . [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified by chiral HPLC (Stationary phase: CHIRALPAK AD-H 5 μM Daicel column, Mobile phase: 75/22/3 heptane/ethanol/methanol (with 7 N ammonia)), followed by basic HPLC to give 2 enantiomers. The first eluting enantiomer was Example 93b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.85-8.82 (m, 1H), 7.91 (d, J=2.2 Hz, 1H), 7.89-7.85 (m, 1H), 7.69 (d, J=8.8 Hz, 1H), 7.65 (d, J=8.2 Hz, 1H), 7.50-7.47 (m, 1H), 7.43-7.38 (m, 1H), 6.40-6.36 (m, 1H), 4.69-4.62 (m, 1H), 4.33-4.27 (m, 4H), 3.43 (s, 3H), 2.42-2.33 (m, 2H), 1.34 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 26 H 25 ClF 3 N 5 O 2 , 531.2; m/z found, 532.0 [M+H] + and the second eluting enantiomer was Example 93c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.85-8.82 (m, 1H), 7.91 (d, J=2.2 Hz, 1H), 7.89-7.85 (m, 1H), 7.69 (d, J=8.8 Hz, 1H), 7.67-7.63 (m, 1H), 7.50-7.46 (m, 1H), 7.40 (dd, J=8.7, 2.2 Hz, 1H), 6.39-6.37 (m, 1H), 4.69-4.61 (m, 1H), 4.32-4.27 (m, 4H), 3.43 (s, 3H), 2.40-2.34 (m, 2H), 1.34 (d, J=6.1 Hz, 6H). MS (ESI): mass calcd. for C 26 H 25 ClF 3 N 5 O 2 , 531.2; m/z found, 532.0 [M+H] + .

Example 94a: [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol

To a sealed tube was added (4-chlorophenyl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)methanol.TFA (110 mg, 0.23 mmol, Example 49a), azetidine (78 μL, 1.15 mmol) and dimethylformamide (1.2 mL). The reaction vessel was sealed and heated in a 100° C. oil bath. After overnight heating, the vessel was cooled to room temperature and the contents concentrated to dryness. The residue was dissolved in EtOAc (15 mL) and washed with saturated aqueous ammonium chloride (2×10 mL). The organics were dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a light yellow oil. The crude material was purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. MS (ESI): mass calcd. for C 26 H 26 Cl 2 N 4 O 2 , 496.1; m/z found, 497.1 [M+H] + . [2-Azetidin-1-yl-4-chloro-3-(1-methylethoxy)quinolin-6-yl](4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol was purified by HPLC (C-18 column, Mobile phase: 1/1 acetonitrile/water+2% ammonium bicarbonate) followed by chiral separation on a CHIRALPAK AD 20 μM Daicel column (Mobile phase: 1/1 EtOH/MeOH) to give 2 enantiomers. The first eluting enantiomer was Example 94b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 7.90 (d, J=2.2 Hz, 1H), 7.65 (d, J=8.8 Hz, 1H), 7.38-7.35 (m, 2H), 7.32-7.30 (m, 4H), 6.39 (s, 1H), 4.67-4.61 (m, 1H), 4.29-4.25 (m, 4H), 3.74 (s, 1H), 3.38 (s, 3H), 2.39-2.33 (m, 2H), 1.33 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 26 H 26 Cl 2 N 4 O 2 , 496.1; m/z found, 497.1 [M+H] + and the second eluting enantiomer was Example 94c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 7.90 (d, J=2.2 Hz, 1H), 7.65 (d, J=8.7 Hz, 1H), 7.40-7.34 (m, 2H), 7.32-7.29 (m, 4H), 6.40 (s, 1H), 4.67-4.61 (m, 1H), 4.30-4.25 (m, 4H), 3.69 (s, 1H), 3.38 (s, 3H), 2.40-2.33 (m, 2H), 1.33 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 26 H 26 Cl 2 N 4 O 2 , 496.1; m/z found, 497.1 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 13 of 15

Example 95a: [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a mixture of [2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol.TFA (125 mg, 0.2 mmol, Example 48) in toluene (2 mL) was added NaOMe (108 mg, 2 mmol) and the resulting mixture heated to 60° C. for 45 minutes. The mixture was then heated to 80° C. and stirred for an additional hour. The mixture was then cooled to room temperature, diluted with DCM and filtered, rinsing the filter cake with DCM. The filtrate was concentrated to dryness and the residue purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a clear colorless oil. MS (ESI): mass calcd. for C 24 H 22 ClF 3 N 4 O 3 , 506.1; m/z found, 507.0 [M+H] + . [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified by achiral SFC (Stationary phase: Whelko-1 column, Mobile phase: 90% CO 2 , 10% MeOH+0.2% iPrNH 2 ) followed by chiral SFC (Stationary phase: IC column, Mobile phase: 88% CO 2 , 12% iPrOH+0.2% iPrNH 2 ) to give 2 enantiomers. The first eluting enantiomer was Example 95b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.83-8.79 (m, 1H), 8.09-8.06 (m, 1H), 7.92-7.88 (m, 1H), 7.78 (d, J=8.6 Hz, 1H), 7.65 (d, J=8.2 Hz, 1H), 7.48-7.44 (m, 1H), 7.33-7.29 (m, 1H), 6.35-6.30 (m, 1H), 4.71-4.65 (m, 1H), 4.11 (s, 3H), 3.36 (s, 3H), 1.38-1.36 (m, 6H). MS (ESI): mass calcd. for C 24 H 22 ClF 3 N 4 O 3 , 506.1; m/z found, 507.0 [M+H] + and the second eluting enantiomer was Example 95c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.84-8.80 (m, 1H), 8.09-8.05 (m, 1H), 7.92-7.87 (m, 1H), 7.79 (d, J=8.8 Hz, 1H), 7.65 (d, J=8.2 Hz, 1H), 7.48-7.44 (m, 1H), 7.36-7.31 (m, 1H), 6.37-6.31 (m, 1H), 4.72-4.64 (m, 1H), 4.11 (s, 3H), 3.36 (s, 3H), 1.38-1.36 (m, 6H). MS (ESI): mass calcd. for C 24 H 22 ClF 3 N 4 O 3 , 506.1; m/z found, 507.0 [M+H] + .

Example 96: [2,4-Dimethoxy-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

Example 97: (4-Chloro-2,3-dimethoxyquinolin-6-yl)(1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

Example 98a: [4-Chloro-2-methoxy-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a mixture of [2,4-dichloro-3-(2,2,2-trifluoroethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol (86 mg, 0.16 mmol, Example 39) in toluene (1.6 mL) was added NaOMe (84 mg, 1.56 mmol) and the resulting mixture heated to 80° C. for 3.5 hours. The mixture was then cooled to room temperature, diluted with DCM and filtered, rinsing the filter cake with DCM. The filtrate was concentrated to dryness and the residue purified by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to provide the title compound as a light yellow oil. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.82-8.80 (m, 1H), 8.11-8.09 (m, 1H), 7.93-7.89 (m, 1H), 7.84-7.80 (m, 1H), 7.69-7.65 (m, 1H), 7.52 (dd, J=8.8, 2.2 Hz, 1H), 7.43-7.41 (m, 1H), 6.44-6.42 (m, 1H), 4.52 (q, J=8.3 Hz, 2H), 4.16 (s, 3H), 4.05 (s, 1H), 3.39 (s, 3H). MS (ESI): mass calcd. for C 23 H 17 ClF 6 N 4 O 3 , 546.1; m/z found, 547.1 [M+H] + . [4-Chloro-2-methoxy-3-(2,2,2-trifluoroethoxy)-6-quinolyl]-(3-methylimidazol-4-yl)-[6-(trifluoromethyl)-3-pyridyl]methanol was purified by chiral SFC (Stationary phase: CHIRALCEL OJ-H 5 μM 250×20 mm, Mobile phase: 85% CO 2 , 15% MeOH+0.3% iPrNH 2 ) to give 2 enantiomers. The first eluting enantiomer was Example 98b: 1 H NMR (600 MHz, CDCl 3 ) δ 8.81 (d, J=2.2 Hz, 1H), 8.09 (d, J=2.2 Hz, 1H), 7.93-7.90 (m, 1H), 7.83 (d, J=8.8 Hz, 1H), 7.69-7.67 (m, 1H), 7.53-7.51 (m, 1H), 7.48 (s, 1H), 6.50-6.47 (m, 1H), 4.55-4.49 (m, 2H), 4.16 (s, 3H), 3.49 (s, 1H), 3.41 (s, 3H). MS (ESI): mass calcd. for C 23 H 17 ClF 6 N 4 O 3 , 546.1; m/z found, 547.0 [M+H] + and the second eluting enantiomer was Example 98c: 1 H NMR (600 MHz, CDCl 3 ) δ 8.83 (d, J=2.2 Hz, 1H), 8.11 (d, J=2.1 Hz, 1H), 7.95-7.91 (m, 1H), 7.83 (d, J=8.7 Hz, 1H), 7.71-7.68 (m, 1H), 7.52 (dd, J=8.8, 2.2 Hz, 1H), 7.50 (s, 1H), 6.50-6.47 (m, 1H), 4.53 (q, J=8.3 Hz, 2H), 4.16 (s, 3H), 3.61 (s, 1H), 3.41 (s, 3H). MS (ESI): mass calcd. for C 23 H 17 ClF 6 N 4 O 3 , 546.1; m/z found, 547.0 [M+H] + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 14 of 15

Example 99a: [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol

To a mixture of (4-chlorophenyl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)methanol.TFA (189 mg, 0.32 mmol, Example 49a) in toluene (3.2 mL) was added NaOMe (173 mg, 3.2 mmol) and the resulting mixture heated to 60° C. for 45 minutes. The mixture was then heated to 80° C. and stirred for an additional hour. The mixture was then cooled to room temperature, diluted with DCM and filtered, rinsing the filter cake with DCM. The filtrate was concentrated to dryness and the residue purified by reverse-phase HPLC (acetonitrile/water+0.05% TFA) to provide the title compound as a tan foam. MS (ESI): mass calcd. for C 24 H 23 Cl 2 N 3 O 3 , 471.1; m/z found, 472.0 [M+H] + . [4-Chloro-2-methoxy-3-(1-methylethoxy)quinolin-6-yl](4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol was purified by achiral SFC (Stationary phase: CHIRALPAK OD-H column, Mobile phase: 90% CO 2 , 10% MeOH+0.2% iPrNH 2 ) followed by chiral SFC (Stationary phase: CHIRALPAK OJ-H column, Mobile phase: 87% CO 2 , 13% iPrOH+0.2% iPrNH 2 ) to give 2 enantiomers. The first eluting enantiomer was Example 99b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.04-8.01 (m, 1H), 7.77 (d, J=8.7 Hz, 1H), 7.48-7.44 (m, 2H), 7.35-7.29 (m, 4H), 6.48-6.43 (m, 1H), 4.70-4.64 (m, 1H), 4.12 (s, 3H), 3.41 (s, 3H), 3.16-3.09 (m, 1H), 1.37 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 24 H 23 Cl 2 N 3 O 3 , 471.1; m/z found, 473.1 [M+H] + and the second eluting enantiomer was Example 99c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.03-8.01 (m, 1H), 7.79-7.75 (m, 1H), 7.48-7.44 (m, 2H), 7.34-7.29 (m, 4H), 6.47-6.44 (m, 1H), 4.71-4.64 (m, 1H), 4.12 (s, 3H), 3.41 (s, 3H), 3.18-3.11 (m, 1H), 1.37 (d, J=6.2 Hz, 6H). MS (ESI): mass calcd. for C 24 H 23 Cl 2 N 3 O 3 , 471.1; m/z found, 473.1 [M+H] + .

Example 100a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol

A solution of n-butyllithium (2.5 M in hexanes, 0.2 mL, 0.5 mmol) was added dropwise by syringe to a solution of 6-bromo-4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinoline (0.24 g, 0.55 mmol, Intermediate 40) in dry deoxygenated THF (12 mL) at −78° C. After 2 minutes, a solution of (1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone (0.13 g, 0.50 mmol, Intermediate 14: step b) in dry THF (4 mL) was added dropwise by syringe. An additional 2 mL of THF was used to complete the quantitative addition. After 10 minutes, the flask was removed from the dry-ice bath and placed into an ice-water bath. After 1 hour, the reaction was quenched with saturated aqueous ammonium chloride solution and the mixture was partitioned between water and EtOAc. The layers were separated and the aqueous phase was further extracted with EtOAc and washed with saturated aqueous NaCl solution. The organic phase was dried (MgSO 4 ), filtered, and concentrated to dryness. The crude product was purified by flash column chromatography (silica gel, 0-7% MeOH-DCM) to provide the title compound. MS m/e 614.1 (M+H).

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol was purified by chiral SFC [ChiralPak IC column, 5 μm, 250 mm×20 mm, mobile phase: 70% carbon dioxide, 30% 2-propanol (containing 0.3% diisopropylamine)] to provide two pure enantiomers. The first eluting enantiomer was Example 100b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.70 (d, J=5.2 Hz, 1H), 8.15 (d, J=2.1 Hz, 1H), 7.88 (dd, J=1.7, 0.9 Hz, 1H), 7.82 (d, J=8.8 Hz, 1H), 7.56 (dd, J=8.8, 2.2 Hz, 1H), 7.50 (dd, J=5.2, 1.7 Hz, 1H), 7.38 (s, 1H), 6.39 (s, 1H), 4.57 (s, 1H), 4.09 (s, 3H), 3.81 (s, 2H), 3.36 (s, 3H), 3.03 (d, J=10.9 Hz, 2H), 2.20 (t, J=11.6 Hz, 2H), 2.04-1.90 (m, 1H), 1.80-1.78 (m, 2H), 1.70-1.47 (m, 2H); MS m/e 614.1 (M+H) and the second eluting enantiomer was Example 100c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.70 (d, J=5.1 Hz, 1H), 8.15 (d, J=2.1 Hz, 1H), 7.89 (dd, J=1.6, 0.8 Hz, 1H), 7.81 (d, J=8.7 Hz, 1H), 7.55 (dd, J=8.8, 2.2 Hz, 1H), 7.50 (dd, J=5.1, 1.7 Hz, 1H), 7.33 (s, 1H), 6.35 (s, 1H), 4.97 (s, 1H), 4.08 (s, 3H), 3.80 (s, 2H), 3.35 (s, 3H), 3.02 (d, J=9.9 Hz, 2H), 2.19 (t, J=11.9 Hz, 2H), 1.99-1.97 (m, 1H), 1.79-1.77 (m, 2H), 1.73-1.46 (m, 2H); MS m/e 614.1 (M+H).

Example 101a: (3-((4-(1H-Pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol

(3-((4-(1H-Pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(4-chlorophenyl)(1-methyl-1H-imidazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 60% CO 2 , 40% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 101b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.11 (d, J=2.1 Hz, 1H), 7.80 (d, J=8.7 Hz, 1H), 7.55 (dd, J=8.8, 2.2 Hz, 1H), 7.49-7.47 (m, 1H), 7.45 (s, 1H), 7.40 (d, J=2.3 Hz, 1H), 7.35-7.28 (m, 4H), 6.44 (d, J=1.2 Hz, 1H), 6.23 (t, J=2.1 Hz, 1H), 4.18-4.11 (m, 1H), 4.10 (s, 3H), 3.86 (s, 2H), 3.41 (s, 3H), 3.06 (d, J=11.5 Hz, 2H), 2.45-2.34 (m, 2H), 2.13-2.04 (m, 2H), 2.02-1.95 (m, 2H); MS m/e 577.5 (M+H) + and the second eluting enantiomer was Example 101c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.11 (d, J=2.2 Hz, 1H), 7.80 (d, J=8.7 Hz, 1H), 7.55 (dd, J=8.8, 2.2 Hz, 1H), 7.48 (d, J=1.7 Hz, 1H), 7.44 (s, 1H), 7.40 (d, J=2.3 Hz, 1H), 7.36-7.28 (m, 4H), 6.44 (d, J=1.2 Hz, 1H), 6.23 (t, J=2.1 Hz, 1H), 4.18-4.10 (m, 1H), 4.10 (s, 3H), 3.85 (s, 2H), 3.41 (s, 3H), 3.06 (d, J=11.7 Hz, 2H), 2.42-2.37 (m, 2H), 2.10-2.07 (m, 2H), 2.1-1.94 (m, 2H); MS m/e 577.5 (M+H) + .

›Example 44: (1-Acetylpiperidin-4-yl)[2,4-dichloro-3-(1-methylethoxy)quinolin-6-yl]phenylmethanol.TFA · 15 of 15

Example 102a: 1-(4-((3-((4-(1H-Pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone

The title compound was prepared analogously to the method described in Example 100a using 3-((4-(1H-pyrazol-1-yl)piperidin-1-yl)methyl)-6-bromo-4-chloro-2-methoxyquinoline (Intermediate 41) and 1-(4-benzoylpiperidin-1-yl)ethanone (Intermediate 7). MS m/e 588.6 (M+H) + . 1-(4-((3-((4-(1H-Pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 55% CO 2 , 45% ethanol). The first eluting enantiomer was Example 102b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.31-8.29 (m, 1H), 7.78-7.74 (m, 1H), 7.70-7.65 (m, 1H), 7.57-7.50 (m, 2H), 7.48 (s, 1H), 7.40 (d, J=2.4 Hz, 1H), 7.36-7.31 (m, 2H), 7.24-7.22 (m, 1H), 6.23-6.22 (m, 1H), 4.72-4.66 (m, 1H), 4.20-4.10 (m, 1H), 4.07 (s, 3H), 3.86-3.80 (m, 3H), 3.17-2.97 (m, 3H), 2.79-2.74 (m, 1H), 2.62-2.54 (m, 1H), 2.42-2.38 (m, 3H), 2.17-1.89 (m, 7H), 1.74-1.25 (m, 4H); MS m/e 588.6 (M+H) + and the second eluting enantiomer was Example 102c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.30 (dd, J=8.3, 2.1 Hz, 1H), 7.79-7.73 (m, 1H), 7.70-7.65 (m, 1H), 7.54-7.51 (m, 2H), 7.49 (d, J=1.8 Hz, 1H), 7.40 (d, J=2.2 Hz, 1H), 7.36-7.31 (m, 2H), 7.25-7.19 (m, 1H), 6.25-6.23 (m, 1H), 4.73-4.66 (m, 1H), 4.16-4.14 (m, 1H), 4.07 (s, 3H), 3.90-3.78 (m, 3H), 3.17-3.00 (m, 3H), 2.82-2.71 (m, 1H), 2.66-2.50 (m, 1H), 2.44-2.36 (m, 2H), 2.31 (s, 1H), 2.15-1.90 (m, 7H), 1.56-1.27 (m, 4H); MS m/e 588.6 (M+H) + .

Example 103a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using (2,6-dimethylpyridin-3-yl)(l-methyl-1H-1,2,3-triazol-5-yl)methanone (Intermediate 11: step b). MS m/e 575.1 (M+H) + . (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 103b: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.07 (s, 1H), 7.86-7.80 (m, 1H), 7.39 (d, J=9.1 Hz, 1H), 7.01-6.91 (m, 3H), 4.10 (s, 3H), 3.94 (s, 3H), 3.81 (s, 2H), 3.38 (s, 1H), 3.03 (d, J=11.4 Hz, 2H), 2.56 (s, 3H), 2.41 (s, 3H), 2.20 (t, J=11.8 Hz, 2H), 1.97 (s, 1H), 1.80-1.78 (m, 2H), 1.59-1.57 (m, 2H); MS m/e 577.5 (M+H) + and the second eluting enantiomer was Example 103c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.08 (s, 1H), 7.85-7.79 (m, 1H), 7.39 (d, J=8.1 Hz, 1H), 6.99-6.91 (m, 3H), 4.10 (s, 3H), 3.94 (s, 3H), 3.81 (s, 2H), 3.46 (s, 1H), 3.03 (d, J=11.5 Hz, 2H), 2.56 (s, 3H), 2.39 (s, 3H), 2.20 (t, J=11.8 Hz, 3H), 1.99 (s, 1H), 1.80-1.78 (m, 2H), 1.61-1.57 (m, 2H); MS m/e 577.5 (M+H) + .

Example 104a: (3-((4-(1H-Pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A solution of n-BuLi (0.3 mL, 0.75 mmol, 2.5 M solution in hexane) was added slowly to a solution of 1-methyl-1H-1,2,3-triazole (65.6 mg, 0.789 mmol) in THF (4 mL) at −45° C. After addition, stirring was continued for an additional 30 minutes at 40° C. and (3-((4-(1H-pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (189 mg, 0.395 mmol, Intermediate 42) dissolved in THF (2 mL) was slowly added. An additional 1 mL of THF was used to complete the quantitative addition. The mixture was stirred at −40° C. for 5 minutes then warmed to room temperature and stirred for 1 hour. The solution was quenched with saturated aqueous NH 4 Cl solution. H 2 O was added and layers were separated. The aqueous layer was extracted with EtOAc and the combined organic extracts washed with brine, dried over MgSO 4 , filtered, evaporated in vacuo. The crude product was purified using flash column chromatography (0 to 7% MeOH-DCM) to provide the title compound. MS m/e 562.6 (M+H) + .

(3-((4-(1H-Pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 m, 250×20 mm, mobile phase: 0.3% isopropyl amine, 70% CO 2 , 30% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 104b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.20 (d, J=2.1 Hz, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.49-7.44 (m, 1H), 7.42-7.34 (m, 2H), 7.18 (s, 1H), 6.23-6.21 (m, 1H), 6.13 (s, 1H), 4.88 (s, 1H), 4.19-4.15 (m, 1H), 4.11 (s, 3H), 3.92 (s, 3H), 3.85 (s, 2H), 3.40 (s, 3H), 3.08 (s, 2H), 2.44-2.38 (m, 2H), 2.33 (s, 3H), 2.15-1.89 (m, 4H); MS m/e 562.6 (M+H) + and the second eluting enantiomer was Example 104c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.20 (d, J=2.2 Hz, 1H), 7.76 (d, J=8.5 Hz, 1H), 7.46 (dd, J=1.7, 0.7 Hz, 1H), 7.43-7.34 (m, 2H), 7.18 (s, 1H), 6.23-6.22 (m, 1H), 6.13 (s, 1H), 4.85 (s, 1H), 4.19-4.13 (m, 1H), 4.11 (s, 3H), 3.92 (s, 3H), 3.85 (s, 2H), 3.41 (s, 3H), 3.08 (s, 2H), 2.44-2.38 (m, 2H), 2.33 (s, 3H), 2.11-1.99 (m, 4H); MS m/e 562.6 (M+H) + .

Example 105a: (3-((4-(1H-Pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using 3-((4-(1H-pyrazol-1-yl)piperidin-1-yl)methyl)-6-bromo-4-chloro-2-methoxyquinoline (Intermediate 41) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (Intermediate 11: step b). MS m/e 573.6 (M+H)+. (3-((4-(1H-pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(l-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 75% CO 2 , 25% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 105b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.05 (s, 1H), 7.84 (d, J=8.8 Hz, 1H), 7.48-7.39 (m, 2H), 7.36 (s, 1H), 7.04-6.95 (m, 2H), 6.93-6.91 (m, 1H), 6.20 (s, 1H), 4.15-4.11 (m, 4H), 3.92 (s, 3H), 3.86 (s, 2H), 3.14-2.98 (m, 3H), 2.54 (s, 3H), 2.49-2.34 (m, 5H), 2.14-1.90 (m, 4H); MS m/e 573.6 (M+H)+ and the second eluting enantiomer was Example 105c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.05 (s, 1H), 7.85-7.83 (m, 1H), 7.48-7.45 (m, 2H), 7.37 (s, 1H), 7.0-6.98 (m, 2H), 6.93-6.91 (m, 1H), 6.20 (s, 1H), 4.16-4.11 (m, 4H), 3.92 (s, 3H), 3.86 (s, 2H), 3.11-2.97 (m, 3H), 2.54 (s, 3H), 2.44-2.38 (d, J=2.9 Hz, 5H), 2.08-1.90 (m, 4H); MS m/e 573.6 (M+H)+.

›Example 106a

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 104a using (4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (Intermediate 43) in place of (3-((4-(1H-pyrazol-1-yl)piperidin-1-yl)methyl)-4-chloro-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone. MS m/e 564.6 (M+H) + . (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 106b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.18 (d, J=2.2 Hz, 1H), 7.78-7.70 (m, 1H), 7.37 (dd, J=8.7, 2.2 Hz, 1H), 7.19 (s, 1H), 6.13 (s, 1H), 4.51 (s, 1H), 4.10 (s, 3H), 3.93 (s, 3H), 3.81 (s, 2H), 3.41 (s, 3H), 3.04 (s, 2H), 2.33 (s, 3H), 2.26-2.15 (m, 2H), 2.06-1.90 (m, 1H), 1.82-1.79 (m, 2H), 1.62-1.56 (m, 2H); MS m/e 564.6 (M+H) + and the second eluting enantiomer was Example 106c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.18 (d, J=2.2 Hz, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.37 (dd, J=8.7, 2.2 Hz, 1H), 7.19 (s, 1H), 6.13 (s, 1H), 4.49 (s, 1H), 4.10 (s, 3H), 3.93 (s, 3H), 3.81 (s, 2H), 3.41 (s, 3H), 3.04 (s, 2H), 2.33 (s, 3H), 2.23-2.17 (m, 2H), 2.01-1.99 (m, 1H), 1.82-1.79 (m, 2H), 1.61-1.58 (m, 2H); MS m/e 564.6 (M+H) + .

›Example 107a

(4-Chloro-3-((4,4-difluoropiperidin-1-yl)methyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using 6-bromo-4-chloro-3-((4,4-difluoropiperidin-1-yl)methyl)-2-methoxyquinoline (Intermediate 44) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (Intermediate 11: step b). MS m/e 543.6 (M+H) + .

(4-Chloro-3-((4,4-difluoropiperidin-1-yl)methyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD, 5 μm, 250×30 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 107b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.08 (d, J=2.2 Hz, 1H), 7.83 (d, J=8.8 Hz, 1H), 7.40 (dd, J=8.7, 2.1 Hz, 1H), 7.02-6.90 (m, 3H), 4.11 (s, 3H), 3.94 (s, 3H), 3.87 (s, 2H), 3.40 (s, 1H), 2.72-2.69 (m, 4H), 2.56 (s, 3H), 2.40 (s, 3H), 2.00-1.91 (m, 4H); MS m/e 543.6 (M+H) + and the second eluting enantiomer was Example 107c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.10-8.06 (m, 1H), 7.83 (dd, J=8.7, 0.5 Hz, 1H), 7.39 (dd, J=8.8, 2.2 Hz, 1H), 7.02-6.90 (m, 3H), 4.11 (s, 3H), 3.94 (s, 3H), 3.86 (s, 2H), 2.72-2.69 (m, 4H), 2.56 (s, 3H), 2.39 (s, 3H), 2.01-1.91 (m, 4H); MS m/e 543.6 (M+H) + .

›Example 108a

(4-Chloro-3-((4-fluoropiperidin-1-yl)methyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using 6-bromo-4-chloro-3-((4-fluoropiperidin-1-yl)methyl)-2-methoxyquinoline (Intermediate 45) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (Intermediate 11: step b). MS m/e 525.6 (M+H) + .

(4-Chloro-3-((4-fluoropiperidin-1-yl)methyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 m, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 108b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.08 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.38 (dd, J=8.8, 2.2 Hz, 1H), 7.02-6.89 (m, 3H), 4.77-4.51 (m, 1H), 4.10 (s, 3H), 3.94 (s, 3H), 3.82 (s, 2H), 3.43 (s, 1H), 2.77-2.71 (m, 2H), 2.56-2.49 (m, 5H), 2.40 (s, 3H), 1.89-1.80 (m, 4H); MS m/e 525.6 (M+H) + and the second eluting enantiomer was Example 108c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.08 (dd, J=2.3, 0.6 Hz, 1H), 7.82 (dd, J=8.8, 0.6 Hz, 1H), 7.38 (dd, J=8.8, 2.2 Hz, 1H), 7.03-6.88 (m, 3H), 4.76-4.51 (m, 1H), 4.10 (s, 3H), 3.94 (s, 3H), 3.82 (s, 2H), 3.58 (s, 1H), 2.75-2.71 (m, 2H), 2.55-2.51 (m, 5H), 2.39 (s, 3H), 1.89-1.80 (m, 4H); MS m/e 525.6 (M+H) + .

›Example 109a

(4-Chloro-3-((3,3-difluoroazetidin-1-yl)methyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using 6-bromo-4-chloro-3-((3,3-difluoroazetidin-1-yl)methyl)-2-methoxyquinoline (Intermediate 46) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (Intermediate 11: step b). MS m/e 515.2 (M+H) + .

(4-Chloro-3-((3,3-difluoroazetidin-1-yl)methyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD, 5 μm, 250×30 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 109b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.08 (dd, J=2.2, 0.6 Hz, 1H), 7.84 (dd, J=8.8, 0.6 Hz, 1H), 7.40 (dd, J=8.8, 2.2 Hz, 1H), 7.03-6.92 (m, 3H), 4.12 (s, 3H), 4.08 (s, 2H), 3.94 (s, 3H), 3.77 (t, J=12.0 Hz, 4H), 3.39 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H); MS m/e 515.2 (M+H) + and the second eluting enantiomer was Example 109c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.13-8.02 (m, 1H), 7.92-7.76 (m, 1H), 7.40 (dd, J=8.8, 2.2 Hz, 1H), 7.06-6.89 (m, 3H), 4.12 (s, 3H), 4.08 (s, 2H), 3.94 (s, 3H), 3.77 (t, J=12.0 Hz, 4H), 3.38 (s, 1H), 2.56 (s, 3H), 2.40 (s, 3H); MS m/e 515.2 (M+H) + .

›Example 110a

(4-Chloro-3-((3-fluoroazetidin-1-yl)methyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using 6-bromo-4-chloro-3-((3-fluoroazetidin-1-yl)methyl)-2-methoxyquinoline (Intermediate 47) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (Intermediate 11: step b). MS m/e 497.1 (M+H) + .

(4-Chloro-3-((3-fluoroazetidin-1-yl)methyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 m, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 110b 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.07 (dd, J=2.2, 0.6 Hz, 1H), 7.83 (dd, J=8.7, 0.5 Hz, 1H), 7.39 (dd, J=8.8, 2.2 Hz, 1H), 7.03-6.91 (m, 3H), 5.23-4.95 (m, 1H), 4.12 (s, 3H), 4.01 (s, 2H), 3.94 (s, 3H), 3.78-3.71 (m, 2H), 3.46-3.31 (m, 3H), 2.56 (s, 3H), 2.39 (s, 3H); MS m/e 497.1 (M+H) + and the second eluting enantiomer was Example 110c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.08 (dd, J=2.2, 0.6 Hz, 1H), 7.83 (dd, J=8.8, 0.6 Hz, 1H), 7.39 (dd, J=8.8, 2.2 Hz, 1H), 7.02-6.91 (m, 3H), 5.23-4.96 (m, 1H), 4.12 (s, 3H), 4.01 (s, 2H), 3.94 (s, 3H), 3.81-3.67 (m, 2H), 3.49-3.34 (m, 3H), 2.56 (s, 3H), 2.39 (s, 3H); MS m/e 497.1 (M+H) + .

›Example 111

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)bis(1,2-dimethyl-1H-imidazol-5-yl)methanol

A solution of n-BuLi (0.306 mL, 0.765 mmol, 2.5 M solution in hexane) was slowly added to a solution of 5-bromo-1,2-dimethyl-1H-imidazole (139 mg, 0.794 mmol) in THF (8 mL) at −78° C. After addition, stirring was continued for an additional 30 minutes and (4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (Intermediate 43, 160 mg, 0.33 mmol) dissolved in THF (4 mL) was slowly added. An additional 1 mL of THF was used to complete the quantitative addition. The mixture was stirred at −78° C. for 5 minutes and the flask was removed from the dry-ice bath and placed into an ice-water bath. After 2 hours of stirring, the solution was quenched with aqueous saturated NH 4 Cl solution. The aqueous layer was extracted with EtOAc and the combined organic extracts washed with brine, dried over MgSO 4 , filtered, evaporated in vacuo. The crude product was purified using flash column chromatography (0 to 10% MeOH-DCM) to provide the title compound. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.22 (s, 1H), 7.67 (d, J=8.7 Hz, 1H), 7.39 (dd, J=8.7, 2.2 Hz, 1H), 6.18 (s, 2H), 4.87 (s, 1H), 4.09 (s, 3H), 3.80 (s, 2H), 3.41 (s, 6H), 3.07-3.05 (m, 2H), 2.31 (s, 6H), 2.23-2.18 (m, 2H), 2.04-1.94 (m, 1H), 1.82-1.79 (m, 2H), 1.69-1.59 (m, 2H); MS m/e 577.2 (M+H) + .

Example 112a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using (1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone (Intermediate 10: step c). MS m/e 614.1 (M+H) + .

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 75% CO 2 , 25% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 112b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.81 (d, J=2.2 Hz, 1H), 8.14 (d, J=2.1 Hz, 1H), 7.91 (dd, J=8.1, 2.2 Hz, 1H), 7.82 (dd, J=8.9, 0.6 Hz, 1H), 7.67 (dd, J=8.3, 0.8 Hz, 1H), 7.55 (dd, J=8.8, 2.2 Hz, 1H), 7.42 (s, 1H), 6.42 (d, J=1.2 Hz, 1H), 4.27 (s, 1H), 4.09 (s, 3H), 3.81 (s, 2H), 3.39 (s, 3H), 3.03 (d, J=11.0 Hz, 2H), 2.19 (t, J=11.8 Hz, 2H), 1.99-1.96 (m, 1H), 1.80-1.77 (m, 2H), 1.60-1.56 (m, 2H); MS m/e 614.1 (M+H) + and the second eluting enantiomer was Example 112c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.81 (d, J=2.2 Hz, 1H), 8.14 (d, J=2.3 Hz, 1H), 7.91 (dd, J=8.2, 2.1 Hz, 1H), 7.84-7.79 (m, 1H), 7.67 (dd, J=8.3, 0.8 Hz, 1H), 7.55 (dd, J=8.8, 2.2 Hz, 1H), 7.43 (s, 1H), 6.42 (d, J=1.1 Hz, 1H), 4.09 (s, 3H), 3.81 (s, 2H), 3.39 (s, 3H), 3.03 (d, J=11.0 Hz, 2H), 2.19 (t, J=11.8 Hz, 2H), 2.04-1.90 (m, 1H), 1.80-1.78 (m, 2H), 1.64-1.57 (m, 2H); MS m/e 614.1 (M+H) + .

›Example 113a

(4-Chloro-2-methoxy-3-((2,2,6,6-tetramethylpiperidin-1-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using 6-bromo-4-chloro-2-methoxy-3-((2,2,6,6-tetramethylpiperidin-1-yl)methyl)quinoline (Intermediate 48) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (Intermediate 11: step b). MS m/e 563.3 (M+H) + .

(4-Chloro-2-methoxy-3-((2,2,6,6-tetramethylpiperidin-1-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 m, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% mixture of methanol-isopropanol 50/50 v/v). The second eluting enantiomer was Example 113b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.09 (d, J=2.2 Hz, 1H), 7.78 (d, J=8.7 Hz, 1H), 7.36 (d, J=8.6 Hz, 1H), 7.03-6.97 (m, 2H), 6.97-6.92 (m, 1H), 4.13 (s, 2H), 4.09 (s, 3H), 4.05 (s, 1H), 3.94 (s, 3H), 2.56 (s, 3H), 2.39 (s, 3H), 1.59-1.52 (m, 2H), 1.49-1.47 (m, 4H), 1.07 (s, 12H); MS m/e 563.3 (M+H) + .

Example 114a: (4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using 6-bromo-4-chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinoline (Intermediate 49) and (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (Intermediate 11: step b). MS m/e 547.2 (M+H) + .

(4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 114b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.09 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.41 (dd, J=8.7, 2.2 Hz, 1H), 6.98-6.92 (m, 3H), 4.12 (s, 3H), 4.11 (s, 1H), 3.95 (s, 2H), 3.94 (s, 3H), 3.63-3.57 (m, 2H), 3.47 (t, J=7.7 Hz, 2H), 3.16 (dq, J=16.3, 8.2 Hz, 1H), 2.55 (s, 3H), 2.39 (s, 3H); MS m/e 547.2 (M+H) + and the second eluting enantiomer was Example 114c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.08 (d, J=2.2 Hz, 1H), 7.83 (d, J=8.7 Hz, 1H), 7.41 (dd, J=8.7, 2.2 Hz, 1H), 7.00-6.93 (m, 3H), 4.12 (s, 3H), 3.99 (s, 2H), 3.93 (s, 3H), 3.66 (br s, 2H), 3.52-3.49 (m, 2H), 3.22-3.18 (m, 1H), 2.56 (s, 3H), 2.40 (s, 3H); MS m/e 547.2 (M+H) + .

›Example 115

(4-Chloro-2-methoxy-3-((4-(methylsulfonyl)piperidin-1-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

SOCl 2 (0.098 mL, 1.36 mmol) was added to a solution of (4-chloro-3-(hydroxymethyl)-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol (Intermediate 50: step g, 60 mg, 0.14 mmol) in DCM (2.7 mL) and the resulting mixture was stirred for 2 hours. The mixture was then concentrated on the rotary evaporator. 4-(methylsulfonyl)piperidine (22.2 mg, 0.136 mmol) and DIPEA (0.094 mL, 0.546 mmol) were then added sequentially to the crude mixture from above in DCE (2.7 mL) and the reaction mixture was stirred for 1 hour at room temperature and for 18 hours at 100° C. The reaction mixture was cooled to room temperature, diluted with EtOAc, washed with aqueous saturated NaHCO 3 solution followed by aqueous saturated NaCl solution, dried (MgSO 4 ), filtered and concentrated. The crude product was purified by reverse-phase HPLC (5-85% CH 3 CN—H 2 O, 0.05% TFA). The product was converted to the free base (neutralized with saturated aqueous NaHCO 3 and extracted with EtOAc) and the organic fractions were concentrated to afford the title compound. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.10 (d, J=2.2 Hz, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.40 (dd, J=8.8, 2.2 Hz, 1H), 7.00-6.92 (m, 2H), 6.91 (s, 1H), 4.24 (s, 1H), 4.10 (s, 3H), 3.93 (s, 3H), 3.84 (s, 2H), 3.14-3.12 (m, 2H), 2.80-2.79 (m, 4H), 2.54 (s, 3H), 2.37 (s, 3H), 2.31-2.26 (m, 2H), 2.09 (d, J=12.2 Hz, 2H), 1.83-1.81 (m, 2H). MS m/e 585.1 (M+H).

›Example 116a

1-(4-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone

The title compound was prepared analogously to the method described in Example 100a using 1-(4-benzoylpiperidin-1-yl)ethanone (Intermediate 7). MS m/e 590.2 (M+H) + . 1-(4-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(phenyl)methyl)piperidin-1-yl)ethanone was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 70% CO 2 , 30% mixture of methanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 116b: 1 H NMR (600 MHz, CDCl 3 , mixture of rotamers) δ ppm 8.30-8.28 (m, 1H), 7.77-7.74 (m, 1H), 7.69-7.65 (m, 1H), 7.53-7.50 (m, 2H), 7.35-7.31 (m, 2H), 7.25-7.19 (m, 1H), 4.72-4.67 (m, 1H), 4.05 (s, 3H), 3.86-3.81 (m, 3H), 3.14-3.06 (m, 1H), 3.04-3.02 (m, 2H), 2.78-2.74 (m, 1H), 2.63-2.53 (m, 1H), 2.22-2.13 (m, 3H), 2.05-2.04 (m, 3H), 1.98-1.95 (m, 1H), 1.79-1.77 (m, 2H), 1.71-1.64 (m, 1H), 1.61-1.54 (m, 2H), 1.48-1.23 (m, 3H); MS m/e 590.2 (M+H) + and the second eluting enantiomer was Example 116c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.30-8.28 (m, 1H), 7.79-7.73 (m, 1H), 7.69-7.65 (m, 1H), 7.56-7.48 (m, 2H), 7.35-7.31 (m, 2H), 7.25-7.18 (m, 1H), 4.72-4.66 (m, 1H), 4.05 (s, 3H), 3.86-3.81 (m, 3H), 3.18-2.95 (m, 3H), 2.78-2.74 (m, 1H), 2.66-2.49 (m, 1H), 2.28-2.12 (m, 3H), 2.05-2.04 (m, 3H) 1.98-1.89 (m, 1H), 1.82-1.72 (m, 2H), 1.71-1.64 (m, 1H), 1.59-1.54 (m, 2H), 1.51-1.28 (m, 3H); MS m/e 590.2 (M+H) + .

Example 117a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-4-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using (1-methyl-1H-1,2,3-triazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone (Intermediate 64: step b). MS m/e 575.2 (M+H) + .

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(2,6-dimethylpyridin-4-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralcel OD-H, 5 m, 250×20 mm, mobile phase: 0.3% isopropyl amine, 75% CO 2 , 25% mixture of ethanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 117b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.07 (dd, J=2.3, 0.6 Hz, 1H), 7.84 (dd, J=8.8, 0.6 Hz, 1H), 7.48 (dd, J=8.8, 2.2 Hz, 1H), 7.14 (s, 1H), 6.90 (s, 2H), 4.10 (s, 3H), 3.87 (s, 3H), 3.81 (s, 2H), 3.29 (s, 1H), 3.03 (d, J=11.5 Hz, 2H), 2.52 (s, 6H), 2.22-2.17 (m, 2H), 2.05-1.88 (m, 1H), 1.80-1.77 (m, 2H), 1.6-1.54 (m, 2H); MS m/e 575.2 (M+H) + and the second eluting enantiomer was Example 117c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.07 (d, J=2.2 Hz, 1H), 7.84 (d, J=8.8 Hz, 1H), 7.48 (dd, J=8.8, 2.2 Hz, 1H), 7.13 (s, 1H), 6.90 (s, 2H), 4.10 (s, 3H), 3.87 (s, 3H), 3.81 (s, 2H), 3.35 (s, 1H), 3.03 (d, J=11.6 Hz, 2H), 2.52 (s, 6H), 2.22-2.17 (m, 2H), 2.05-1.90 (m, 1H), 1.80-1.77 (m, 2H), 1.6-1.55 (m, 2H); MS m/e 575.2 (M+H) + .

Example 118: (4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)bis(1,2-dimethyl-1H-imidazol-5-yl)methanol

A solution of n-BuLi (0.37 mL, 0.92 mmol, 2.5 M solution in hexane) was slowly added to a solution of 5-bromo-1,2-dimethyl-1H-imidazole (168 mg, 0.959 mmol) in THF (8 mL) at −78° C. After addition, stirring was continued for an additional 25 minutes and (4-chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (Intermediate 52, 182 mg, 0.402 mmol) dissolved in THF (6 mL) was slowly added. An additional 2 mL of THF was used to complete the quantitative addition. The mixture was stirred at −78° C. for 5 minutes and the flask was removed from the dry-ice bath and placed into an ice-water bath. After 18 hours of stirring, the solution was quenched with aqueous saturated NH 4 Cl solution. The aqueous layer was extracted with EtOAc and the combined organic extracts washed with brine, dried over MgSO 4 , filtered, evaporated in vacuo. The crude product was purified by reverse-phase HPLC (5-85% CH 3 CN—H 2 O, 0.05% TFA). The product was converted to the free base (neutralized with saturated aqueous NaHCO 3 and extracted with DCM) and the organic fractions were concentrated to afford the title compound. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.19 (d, J=2.1 Hz, 1H), 7.72 (d, J=8.7 Hz, 1H), 7.43 (dd, J=8.6, 2.1 Hz, 1H), 6.21 (s, 2H), 4.37 (s, 1H), 4.11 (s, 3H), 3.95 (s, 2H), 3.62-3.59 (m, 2H), 3.49-3.46 (m, 2H), 3.41 (s, 6H), 3.20-3.12 (m, 1H), 2.32 (s, 6H); MS m/e 549.2 (M+H) + .

›Example 119a · 1 of 8

(4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

A solution of n-BuLi (0.37 mL, 0.92 mmol, 2.5 M solution in hexane) was added slowly to a solution of 1-methyl-1H-1,2,3-triazole (79.7 mg, 0.959 mmol) in THF (10 mL) at −50° C. After addition, stirring was continued for an additional 30 minutes at −50° C. and (4-chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (Intermediate 52, 182 mg, 0.402 mmol) dissolved in THF (4 mL) was slowly added. An additional 2 mL of THF was used to complete the quantitative addition. The mixture was stirred at −50° C. for 10 minutes then warmed to room temperature and stirred for 18 hours. The solution was quenched with saturated aqueous NH 4 Cl solution. H 2 O was added and layers were separated. The aqueous layer was extracted with EtOAc and the combined organic extracts washed with brine, dried over MgSO 4 , filtered, evaporated in vacuo. The crude product was purified using flash column chromatography (0 to 7% MeOH-DCM) to provide the title compound. MS m/e 536.1 (M+H) + .

(4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 m, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% methanol). The first eluting enantiomer was Example 119b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.19 (d, J=2.2 Hz, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.39 (dd, J=8.7, 2.2 Hz, 1H), 7.13 (s, 1H), 6.09 (s, 1H), 5.46 (s, 1H), 4.12 (s, 3H), 3.94 (s, 2H), 3.91 (s, 3H), 3.66-3.56 (m, 2H), 3.49-3.45 (m, 2H), 3.38 (s, 3H), 3.23-3.08 (m, 1H), 2.28 (s, 3H); MS m/e 536.1 (M+H) + and the second eluting enantiomer was Example 119c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.18 (d, J=2.1 Hz, 1H), 7.77 (d, J=8.7 Hz, 1H), 7.40 (dd, J=8.7, 2.2 Hz, 1H), 7.16 (s, 1H), 6.12 (s, 1H), 5.02 (s, 1H), 4.12 (s, 3H), 3.95 (s, 2H), 3.92 (s, 3H), 3.63-3.59 (m, 2H), 3.49-3.45 (m, 2H), 3.40 (s, 3H), 3.23-3.10 (m, 1H), 2.32 (s, 3H); MS m/e 536.1 (M+H) + .

Example 120a: (4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using 6-bromo-4-chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinoline (Intermediate 49) and (1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone (Intermediate 10: step c). MS m/e 586 (M+H) + .

(4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% ethanol). The first eluting enantiomer was Example 120b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.82 (d, J=2.3 Hz, 1H), 8.16 (d, J=2.2 Hz, 1H), 7.91 (dd, J=8.1, 2.2 Hz, 1H), 7.82 (d, J=8.8 Hz, 1H), 7.67 (dd, J=8.3, 0.8 Hz, 1H), 7.57 (dd, J=8.8, 2.2 Hz, 1H), 7.53 (s, 1H), 6.41 (s, 1H), 4.12 (s, 3H), 4.03 (s, 2H), 3.75-3.69 (m, 2H), 3.59-3.51 (m, 2H), 3.41 (s, 3H), 3.26-3.22 (m, 1H); MS m/e 586 (M+H) + and the second eluting enantiomer was Example 120c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.82 (d, J=2.3 Hz, 1H), 8.16 (d, J=2.2 Hz, 1H), 7.91 (dd, J=8.3, 2.2 Hz, 1H), 7.82 (d, J=8.7 Hz, 1H), 7.67 (dd, J=8.4, 0.8 Hz, 1H), 7.57 (dd, J=8.8, 2.2 Hz, 1H), 7.51 (s, 1H), 6.40 (s, 1H), 4.12 (s, 3H), 4.03 (s, 2H), 3.75-3.69 (m, 2H), 3.55-3.52 (m, 2H), 3.41 (s, 3H), 3.26-3.22 (m, 1H); MS m/e 586 (M+H) + .

Example 121a: (4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol

The title compound was prepared analogously to the method described in Example 100a, using 6-bromo-4-chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinoline (Intermediate 49) and (1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone (Intermediate 14: step b). MS m/e 586 (M+H) + .

(4-Chloro-2-methoxy-3-((3-(trifluoromethyl)azetidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol was purified by chiral SFC (Chiralcel OJ-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% isopropanol). The first eluting enantiomer was Example 121b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.72 (d, J=5.1 Hz, 1H), 8.15 (d, J=2.2 Hz, 1H), 7.88-7.86 (m, 1H), 7.83 (d, J=8.8 Hz, 1H), 7.57 (dd, J=8.8, 2.2 Hz, 1H), 7.50 (dd, J=5.1, 1.6 Hz, 1H), 7.47 (s, 1H), 6.42 (s, 1H), 4.12 (s, 3H), 4.04 (s, 2H), 3.76-3.69 (m, 2H), 3.59-3.52 (m, 2H), 3.39 (s, 3H), 3.27-3.24 (m, 1H); MS m/e 586 (M+H) + and the second eluting enantiomer was Example 121c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.71 (d, J=5.1 Hz, 1H), 8.16 (d, J=2.2 Hz, 1H), 7.91-7.86 (m, 1H), 7.83 (d, J=8.7 Hz, 1H), 7.57 (dd, J=8.8, 2.2 Hz, 1H), 7.50 (d, J=5.1 Hz, 1H), 7.48 (s, 1H), 6.41 (s, 1H), 4.12 (s, 3H), 4.05 (s, 2H), 3.77-3.7 (m, 2H), 3.59-3.52 (m, 2H), 3.39 (s, 3H), 3.32-3.19 (m, 1H); MS m/e 586 (M+H) + .

Example 122a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-1,2,3-triazol-5-yl)(tetrahydro-2H-pyran-4-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using (1-methyl-1H-1,2,3-triazol-5-yl)(tetrahydro-2H-pyran-4-yl)methanone (Intermediate 53: step b). MS m/e 554.1 (M+H) + .

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-1,2,3-triazol-5-yl)(tetrahydro-2H-pyran-4-yl)methanol was purified by chiral SFC (Chiralpak AD-H, 5 m, 250×20 mm, mobile phase: 0.3% isopropyl amine, 83% CO 2 , 17% methanol). The first eluting enantiomer was Example 122b: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.13 (d, J=2.1 Hz, 1H), 7.81-7.73 (m, 2H), 7.37 (dd, J=8.8, 2.1 Hz, 1H), 4.12-4.08 (m, 4H), 3.95-3.88 (m, 1H), 3.82 (s, 2H), 3.74 (s, 3H), 3.53 (td, J=11.6, 2.0 Hz, 1H), 3.34 (td, J=12.0, 2.2 Hz, 1H), 3.06-3.03 (m, 2H), 2.57 (s, 1H), 2.55-2.46 (m, 1H), 2.23-2.17 (m, 2H), 2.03-1.92 (m, 2H), 1.81-1.78 (m, 2H), 1.68-1.52 (m, 2H), 1.52-1.37 (m, 1H), 1.06-1.03 (m, 1H); MS m/e 554.1 (M+H) + and the second eluting enantiomer was Example 122c: 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.13 (d, J=2.1 Hz, 1H), 7.81-7.74 (m, 2H), 7.37 (dd, J=8.7, 2.1 Hz, 1H), 4.12-4.08 (m, 4H), 3.94-3.92 (m, 1H), 3.82 (s, 2H), 3.74 (s, 3H), 3.53 (td, J=11.8, 2.1 Hz, 1H), 3.34 (td, J=11.9, 2.2 Hz, 1H), 3.06-3.04 (m, 2H), 2.57 (s, 1H), 2.56-2.48 (m, 1H), 2.28-2.12 (m, 2H), 2.0-1.97 (m, 2H), 1.81-1.78 (m, 2H), 1.69-1.52 (m, 2H), 1.51-1.37 (m, 1H), 1.06-1.03 (m, 1H); MS m/e 554.1 (M+H) + .

›Example 119a · 2 of 8

Example 123a: 1-(3-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)azetidin-1-yl)ethanone

TFA (0.441 mL, 5.75 mmol) was added to a solution of tert-butyl-3-((4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)azetidine-1-carboxylate (Example 178, 360 mg, 0.576 mmol) in DCM (5.8 mL) and the mixture was heated to 40° C. After stirring for 4 hours, the mixture was cooled to room temperature, diluted with DCM, washed with aqueous saturated NaHCO 3 solution and aqueous saturated sodium chloride solution. The organic layer was dried over MgSO 4 , filtered, and concentrated. TEA (0.37 mL, 2.6 mmol) and Ac 2 O (0.20 mL, 2.1 mmol) were added sequentially to the crude mixture (280 mg, 0.53 mmol) above in DCM (10.7 mL). After stirring for 2 hours, the mixture was cooled to room temperature, diluted with DCM, washed with aqueous saturated NaHCO 3 solution and aqueous saturated sodium chloride solution. The organic layer was dried over MgSO 4 , filtered, and concentrated. The crude product was purified using flash column chromatography (silica gel, 0 to 6% MeOH-DCM) to provide the title compound. MS m/e 567.2 (M+H) + .

1-(3-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)azetidin-1-yl)ethanone was purified by chiral SFC (Chiralpak IC, 5 μm, 250×30 mm, mobile phase: 0.3% isopropyl amine, 55% CO 2 , 45% ethanol). The first eluting enantiomer was Example 123b: 1 H NMR (500 MHz, CDCl 3 , mixture of rotamers) δ ppm 8.31 (d, J=2.1 Hz, 0.6H), 8.24 (d, J=2.1 Hz, 0.4H), 7.79-7.77 (m, 1H), 7.58-7.57 (m, 1H), 7.39-7.35 (m, 1H), 5.44 (s, 0.6H), 5.02 (s, 0.4H), 4.38-4.35 (m, 0.4H), 4.31-4.29 (m, 0.6H), 4.20-4.14 (m, 1H), 4.1-4.08 (m, 3.5H), 4.02-3.99 (m, 0.5H), 3.82-3.81 (m, 2H), 3.79-3.72 (m, 1H), 3.71-3.69 (m, 3H), 3.60-3.45 (m, 1H), 3.05-3.02 (m, 2H), 2.25-2.13 (m, 2H), 2.02-1.97 (m, 1H), 1.83 (s, 1.8H), 1.82-1.77 (m, 3.2H), 1.66-1.47 (m, 2H); MS m/e 567.2 (M+H) + and the second eluting enantiomer was Example 123c: 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.31 (d, J=2.1 Hz, 0.6H), 8.24 (d, J=2.1 Hz, 0.4H), 7.79-7.77 (m, 1H), 7.58-7.57 (m, 1H), 7.39-7.35 (m, 1H), 5.33 (s, 0.6H), 4.92 (s, 0.4H), 4.38-4.35 (m, 0.4H), 4.30-4.27 (m, 0.6H), 4.24-4.12 (m, 1H), 4.11-4.08 (m, 3.5H), 4.02-3.99 (m, 0.5H), 3.82-3.81 (m, 2H), 3.79-3.73 (m, 1H), 3.71-3.69 (m, 3H), 3.56-3.41 (m, 1H), 3.09-2.96 (m, 2H), 2.25-2.13 (m, 2H), 2.02-1.96 (m, 1H), 1.83 (s, 1.8H), 1.82-1.77 (s, 3.2H), 1.66-1.50 (m, 2H); MS m/e 567.2 (M+H) + .

Example 124a: 1-(3-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)azetidin-1-yl)ethanone

TFA (0.396 mL, 5.17 mmol) was added to a solution of tert-butyl-3-((4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)azetidine-1-carboxylate (Example 179, 330 mg, 0.517 mmol) in DCM (5.2 mL) and the mixture was heated to 40° C. After stirring for 4 hours, the mixture was cooled to room temperature, diluted with DCM, washed with aqueous saturated NaHCO 3 solution and aqueous saturated sodium chloride solution. The organic layer was dried over MgSO 4 , filtered, and concentrated. TEA (0.19 mL, 1.4 mmol) and Ac 2 O (0.10 mL, 1.2 mmol) were added sequentially to the crude mixture (150 mg, 0.279 mmol) above in DCM (5.6 mL). After stirring for 1 hour, the mixture was cooled to room temperature, diluted with DCM, washed with aqueous saturated NaHCO 3 solution and aqueous saturated sodium chloride solution. The organic layer was dried over MgSO 4 , filtered, and concentrated. The crude product was purified using flash column chromatography (silica gel, 0 to 6% MeOH-DCM) to provide the title compound. MS m/e 580.2 (M+H) + .

1-(3-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(hydroxy)methyl)azetidin-1-yl)ethanone was purified by chiral SFC (Chiralpak AD-H, 5 μm, 250×30 mm, mobile phase: 0.3% isopropyl amine, 75% CO 2 , 25% mixture of ethanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 124b: 1 H NMR (600 MHz, CDCl 3 , mixture of rotamers) δ ppm 8.32-8.28 (m, 1H), 7.77-7.76 (m, 1H), 7.45-7.41 (m, 1H), 6.82-6.81 (m, 1H), 4.39-4.36 (m, 0.5H), 4.25-4.18 (m, 1H), 4.15-4.13 (m, 0.5H), 4.08 (s, 3H), 4.04-4.01 (m, 0.5H), 3.99-3.96 (m, 0.5H), 3.82 (s, 2H), 3.69-3.67 (m, 0.5H), 3.62-3.58 (m, 0.5H), 3.50-3.42 (m, 1H), 3.12 (s, 3H), 3.07-3.05 (m, 2H), 2.27-2.25 (m, 3H), 2.24-2.16 (m, 2H), 2.05-1.92 (m, 1H), 1.85-1.83 (m, 3H), 1.81-1.79 (m, 2H), 1.61-1.58 (m, 2H); MS m/e 580.2 (M+H) + and the second eluting enantiomer was Example 124c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.32-8.28 (m, 1H), 7.77-7.76 (m, 1H), 7.45-7.41 (m, 1H), 6.83-6.79 (m, 1H), 4.38-4.35 (m, 0.5H), 4.25-4.17 (m, 1H), 4.15-4.13 (0.5H), 4.08 (s, 3H), 4.04-4.01 (m, 0.5H), 3.99-3.96 (m, 0.5H), 3.82 (s, 2H), 3.69-3.66 (m, 0.5H), 3.61-3.58 (m, 0.5H), 3.47-3.44 (m, 1H), 3.13-3.12 (m, 3H), 3.07-3.05 (m, 2H), 2.26-2.24 (m, 3H), 2.23-2.18 (m, 2H), 2.03-1.94 (m, 1H), 1.84-1.83 (m, 3H), 1.83-1.79 (m, 2H), 1.63-1.56 (m, 2H); MS m/e 580.2 (M+H) + .

Example 125a: 1-(3-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(2-(trifluoromethyl)pyridin-4-yl)methyl)azetidin-1-yl)ethanone

The title compound was prepared analogously to the method described in Example 123a using tert-butyl-3-((4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(2-(trifluoromethyl)pyridin-4-yl)methyl)azetidine-1-carboxylate (Example 180). MS m/e 631.1 (M+H) + .

1-(3-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(2-(trifluoromethyl)pyridin-4-yl)methyl)azetidin-1-yl)ethanone was purified by chiral SFC (Chiralpak AD, 5 μm, 250×30 mm, mobile phase: 80% CO 2 , 20% methanol). The first eluting enantiomer was Example 125b: 1 H NMR (600 MHz, CDCl 3 , mixture of rotamers) δ ppm 8.68-8.66 (m, 1H), 8.15 (d, J=2.2 Hz, 0.4H), 8.05 (d, J=2.1 Hz, 0.6H), 7.83-7.70 (m, 2H), 7.54-7.41 (m, 2H), 4.30-4.23 (m, 1H), 4.19-4.11 (m, 1H), 4.08-4.07 (m, 3H), 4.06-3.92 (m, 2H), 3.88-3.87 (m, 1H), 3.82-3.81 (m, 2H), 3.79-3.74 (m, 1H), 3.06-2.97 (m, 2H), 2.24-2.14 (m, 2H), 2.00-1.96 (m, 1H), 1.84 (s, 1.8H), 1.83-1.76 (m, 2H), 1.75 (s, 1.2H), 1.67-1.50 (m, 2H); MS m/e 631.1 (M+H) + and the second eluting enantiomer was Example 125c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.68-8.66 (m, 1H), 8.16 (d, J=2.1 Hz, 0.4H), 8.05 (d, J=2.1 Hz, 0.6H), 7.81-7.78 (m, 1.6H), 7.73-7.72 (m, 0.4H), 7.53-7.42 (m, 2H), 4.30-4.22 (m, 1H), 4.18-4.11 (m, 1H), 4.08-4.07 (m, 3H), 4.06-3.92 (m, 3H), 3.82-3.81 (m, 2H), 3.79-3.74 (m, 1H), 3.06-2.96 (m, 2H), 2.22-2.16 (m, 2H), 2.01-1.96 (m, 1H), 1.84 (s, 1.8H), 1.80-1.76 (m, 2H), 1.74 (s, 1.2H), 1.65-1.49 (m, 2H); MS m/e 631.1 (M+H) + .

›Example 119a · 3 of 8

Example 126a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using (1,2-dimethyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone (Intermediate 60: step b). MS m/e 628 (M+H) + .

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol was purified by chiral SFC (Chiralcel OD-H, 5 m, 250×20 mm, mobile phase: 0.3% isopropyl amine, 75% CO 2 , 25% methanol). The first eluting enantiomer was Example 126b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.69 (d, J=5.2 Hz, 1H), 8.16 (d, J=2.1 Hz, 1H), 7.90-7.88 (m, 1H), 7.82-7.76 (m, 1H), 7.56 (dd, J=8.8, 2.2 Hz, 1H), 7.50 (dd, J=5.1, 1.7 Hz, 1H), 6.21 (s, 1H), 4.08 (s, 3H), 3.79 (s, 2H), 3.24 (s, 3H), 3.05-2.95 (m, 2H), 2.25 (s, 3H), 2.20-2.18 (m, 2H), 2.00-1.95 (m, 1H), 1.79-1.77 (m, 2H), 1.62-1.53 (m, 2H); MS m/e 628 (M+H) + and the second eluting enantiomer was Example 126c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.68 (d, J=5.1 Hz, 1H), 8.17 (d, J=2.1 Hz, 1H), 7.90-7.89 (m, 1H), 7.79 (d, J=8.7 Hz, 1H), 7.56 (dd, J=8.7, 2.2 Hz, 1H), 7.50 (dd, J=5.1, 1.7 Hz, 1H), 6.19 (s, 1H), 4.08 (s, 3H), 3.79 (s, 2H), 3.24 (s, 3H), 3.03-2.99 (m, 2H), 2.24 (s, 3H), 2.20-2.16 (m, 2H), 2.00-1.95 (m, 1H), 1.81-1.75 (m, 2H), 1.64-1.52 (m, 2H); MS m/e 628 (M+H) + .

Example 127a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-1,2,3-triazol-5-yl)(1-(methylsulfonyl)azetidin-3-yl)methanol

TFA (0.39 mL, 5.1 mmol) was added to a solution of tert-butyl-3-((4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)azetidine-1-carboxylate (Example 178, 320 mg, 0.512 mmol) in DCM (5.2 mL) and the mixture was heated to 40° C. After stirring for 4 hours, the mixture was cooled to room temperature, diluted with DCM, washed with aqueous saturated NaHCO 3 solution and aqueous saturated sodium chloride solution. The organic layer was dried over MgSO 4 , filtered, and concentrated. TEA (0.19 mL, 1.4 mmol) and methylsulfonyl chloride (0.05 mL, 0.68 mmol) were added sequentially to the crude mixture (240 mg, 0.46 mmol) above in DCM (9.1 mL). After stirring for 4 hours, the mixture was diluted with DCM, washed with aqueous saturated NaHCO 3 solution and aqueous saturated sodium chloride solution. The organic layer was dried over MgSO 4 , filtered, and concentrated. The crude product was purified using flash column chromatography (0 to 5% MeOH-DCM) to provide the title compound. MS m/e 603.2 (M+H) + . (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-1,2,3-triazol-5-yl)(1-(methylsulfonyl)azetidin-3-yl)methanol was purified by chiral SFC (Chiralcel OD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 60% CO 2 , 40% methanol). The first eluting enantiomer was Example 127b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.25 (d, J=2.1 Hz, 1H), 7.79 (d, J=8.7 Hz, 1H), 7.53 (s, 1H), 7.34 (dd, J=8.7, 2.1 Hz, 1H), 4.39 (s, 1H), 4.23-4.20 (m, 1H), 4.15-4.10 (m, 2H), 4.08 (s, 3H), 3.82 (s, 2H), 3.69 (s, 3H), 3.61-3.59 (m, 1H), 3.58-3.52 (m, 1H), 3.05-3.03 (m, 2H), 2.89 (s, 3H), 2.25-2.15 (m, 2H), 2.01-1.94 (m, 1H), 1.81-1.79 (m, 2H), 1.61-1.56 (m, 2H); MS m/e 603.2 (M+H) + and the second eluting enantiomer was Example 127c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.24 (d, J=2.1 Hz, 1H), 7.79 (d, J=8.7 Hz, 1H), 7.54 (s, 1H), 7.34 (dd, J=8.7, 2.1 Hz, 1H), 4.24-4.21 (m, 1H), 4.14-4.09 (m, 1H), 4.08 (s, 3H), 3.82 (s, 2H), 3.69 (s, 3H), 3.63-3.60 (m, 1H), 3.58-3.52 (m, 1H), 3.05-3.03 (m, 2H), 2.88 (s, 3H), 2.25-2.16 (m, 2H), 2.02-1.95 (s, 1H), 1.81-1.79 (m, 2H), 1.61-1.55 (m, 2H); MS m/e 603.2 (M+H) + .

Example 128a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6yl)(tetrahydro-2H-pyran-4-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using (tetrahydro-2H-pyran-4-yl)(2-(trifluoromethyl)pyridin-4-yl)methanone (Intermediate 61: step b). MS m/e 618.2 (M+H) + .

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6yl)(tetrahydro-2H-pyran-4-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol was purified by chiral SFC (Chiralcel OD-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 70% CO 2 , 30% isopropanol). The first eluting enantiomer was Example 128b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.66 (d, J=5.2 Hz, 1H), 8.30 (d, J=2.1 Hz, 1H), 7.88 (s, 1H), 7.83 (d, J=8.8 Hz, 1H), 7.73 (dd, J=8.9, 2.2 Hz, 1H), 7.64 (dd, J=5.1, 1.7 Hz, 1H), 4.07 (s, 3H), 4.05-4.02 (dd, J=11.6, 3.5 Hz, 2H), 3.81 (s, 2H), 3.54-3.43 (m, 2H), 3.03-3.01 (m, 2H), 2.82-2.79 (m, 1H), 2.41 (s, 1H), 2.22-2.17 (m, 2H), 1.98-1.96 (m, 1H), 1.79-1.77 (m, 2H), 1.72-1.59 (m, 1H), 1.60-1.46 (m, 2H), 1.29-1.14 (m, 2H); MS m/e 618.2 (M+H) + and the second eluting enantiomer was Example 128c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.66 (d, J=5.2 Hz, 1H), 8.32-8.27 (m, 1H), 7.88 (m, 1H), 7.85-7.80 (m, 1H), 7.73 (dd, J=8.8, 2.2 Hz, 1H), 7.64 (dd, J=5.3, 1.7 Hz, 1H), 4.07 (s, 3H), 4.04-4.02 (m, 2H), 3.81 (s, 2H), 3.54-3.43 (m, 2H), 3.03-3.01 (m, 2H), 2.86-2.77 (m, 1H), 2.41 (s, 1H), 2.21-2.17 (m, 2H), 1.99-1.96 (m, 1H), 1.79-1.77 (m, 2H), 1.67-1.64 (m, 1H), 1.59-1.46 (m, 2H), 1.27-1.17 (m, 2H); MS m/e 618.2 (M+H) + .

Example 129a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-1,2,3-triazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1-methyl-1H-1,2,3-triazol-5-yl)(2-(trifluoromethyl)pyridin-4-yl)methanol was purified by chiral SFC (Chiralcel OJ-H, 5 μm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% isopropanol). The first eluting enantiomer was Example 129b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.77 (d, J=5.1 Hz, 1H), 8.04 (d, J=2.3 Hz, 1H), 7.87 (d, J=8.7 Hz, 1H), 7.83-7.82 (m, 1H), 7.47 (dd, J=8.7, 2.3 Hz, 1H), 7.45-7.42 (m, 1H), 7.13 (s, 1H), 4.10 (s, 3H), 3.86 (s, 3H), 3.80 (s, 2H), 3.76 (s, 1H), 3.02-3.00 (m, 2H), 2.23-2.15 (m, 2H), 1.99-1.97 (m, 1H), 1.79-1.77 (m, 2H), 1.60-1.55 (m, 2H); MS m/e 615 (M+H) + and the second eluting enantiomer was Example 129c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.76 (d, J=5.1 Hz, 1H), 8.05 (d, J=2.2 Hz, 1H), 7.86 (d, J=8.8 Hz, 1H), 7.84-7.83 (m, 1H), 7.47 (dd, J=8.7, 2.2 Hz, 1H), 7.44 (dd, J=5.1, 1.7 Hz, 1H), 7.07 (s, 1H), 4.33 (s, 1H), 4.10 (s, 3H), 3.84 (s, 3H), 3.80 (s, 2H), 3.02-3.00 (m, 2H), 2.21-2.17 (m, 2H), 2.00-1.95 (s, 1H), 1.84-1.72 (m, 2H), 1.64-1.49 (m, 2H); MS m/e 615 (M+H) + .

›Example 119a · 4 of 8

Example 130a: (4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(tetrahydro-2H-pyran-4-yl)methanol

The title compound was prepared analogously to the method described in Example 100a using (1,2-dimethyl-1H-imidazol-5-yl)(tetrahydro-2H-pyran-4-yl)methanone (Intermediate 62). MS m/e 567.4 (M+H) + .

(4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(tetrahydro-2H-pyran-4-yl)methanol was purified by chiral SFC (Chiralcel OD-H, 5 m, 250×20 mm, mobile phase: 0.3% isopropyl amine, 60% CO 2 , 40% isopropanol). The first eluting enantiomer was Example 130b: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.16 (s, 1H), 7.75 (d, J=8.5 Hz, 1H), 7.42 (d, J=8.6 Hz, 1H), 7.08 (s, 1H), 4.09-4.04 (m, 4H), 3.89 (dd, J=11.6, 4.2 Hz, 1H), 3.82 (s, 2H), 3.54-3.50 (m, 1H), 3.35-3.30 (m, 1H), 3.14 (s, 3H), 3.06-3.04 (m, 2H), 2.49-2.41 (m, 1H), 2.28-2.27 (m, 4H), 2.22-2.18 (m, 2H), 2.14-2.12 (m, 1H), 2.0-1.98 (m, 1H), 1.83-1.77 (m, 2H), 1.71-1.52 (m, 3H), 1.45-1.35 (m, 1H), 1.04-1.02 (m, 1H); MS m/e 567.4 (M+H) + and the second eluting enantiomer was Example 130c: 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.16 (s, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.42 (d, J=8.5 Hz, 1H), 7.08 (s, 1H), 4.09-4.05 (m, 4H), 3.89 (dd, J=11.6, 3.9 Hz, 1H), 3.82 (s, 2H), 3.55-3.48 (m, 1H), 3.35-3.30 (m, 1H), 3.14 (s, 3H), 3.06-3.04 (m, 2H), 2.50-2.41 (m, 1H), 2.28-2.27 (m, 3H), 2.25-2.17 (m, 2H), 2.14-2.12 (m, 1H), 2.00-1.98 (m, 1H), 1.81-1.79 (m, 2H), 1.69-1.52 (m, 3H), 1.41-1.38 (m, 1H), 1.04-1.02 (m, 1H); MS m/e 567.4 (M+H) + .

Example 131a: 1-(3-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(6-(trifluoromethyl)pyridin-3-yl)methyl)azetidin-1-yl)ethanone

The title compound was prepared analogously to the method described in Example 123a using tert-butyl-3-((4-chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(6-(trifluoromethyl)pyridin-3-yl)methyl)azetidine-1-carboxylate (Example 181). MS m/e 631 (M+H) + .

1-(3-((4-Chloro-2-methoxy-3-((4-(trifluoromethyl)piperidin-1-yl)methyl)quinolin-6-yl)(hydroxy)(6-(trifluoromethyl)pyridin-3-yl)methyl)azetidin-1-yl)ethanone was purified by chiral SFC (Chiralpak AD-H, 5 rpm, 250×20 mm, mobile phase: 0.3% isopropyl amine, 80% CO 2 , 20% mixture of ethanol-isopropanol 50/50 v/v). The first eluting enantiomer was Example 131b: 1 H NMR (600 MHz, CDCl 3 , mixture of rotamers) δ ppm 8.77 (d, J=2.1 Hz, 0.6H), 8.71-8.68 (m, 0.4H), 8.18 (d, J=2.1 Hz, 0.4H), 8.08 (d, J=2.1 Hz, 0.6H), 7.95-7.93 (m, 1H), 7.81-7.79 (m, 1H), 7.67-7.61 (m, 1H), 7.49-7.43 (m, 1H), 4.27-4.14 (m, 2H), 4.07 (s, 3H), 4.06-3.99 (m, 2H), 3.82-3.81 (m, 2H), 3.80-3.68 (m, 2H), 3.05-2.97 (m, 2H), 2.25-2.13 (m, 2H), 2.00-1.95 (m, 1H), 1.83 (s, 1.8H), 1.82-1.77 (m, 2H), 1.76 (s, 1.2H), 1.64-1.49 (m, 2H); MS m/e 631 (M+H) + and the second eluting enantiomer was Example 131c: 1 H NMR (600 MHz, CDCl 3 , mixture of rotamers) δ ppm 8.77 (d, J=2.2 Hz, 0.6H), 8.69 (d, J=2.1 Hz, 0.4H), 8.18 (d, J=2.1 Hz, 0.4H), 8.08 (d, J=2.1 Hz, 0.6H), 7.95-7.93 (m, 1H), 7.81-7.79 (m, 1H), 7.68-7.61 (m, 1H), 7.49-7.46 (m, 1H), 4.28-4.14 (m, 2H), 4.07 (s, 3H), 4.06-3.99 (m, 2H), 3.82-3.81 (m, 2H), 3.81-3.78 (m, 1H), 3.64 (s, 0.4H), 3.55 (s, 0.6H), 3.06-2.99 (m, 2H), 2.24-2.14 (m, 2H), 2.01-1.94 (m, 1H), 1.83 (s, 1.8H), 1.82-1.76 (m, 3.2H), 1.62-1.52 (m, 2H); MS m/e 631 (M+H) + .

Example 132a: (4-Chloro-3-isopropyl-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

To a flask containing 6-bromo-4-chloro-3-isopropyl-2-methoxyquinoline (1.0 g, 3.18 mmol, Intermediate 67: step b) was added THF (25 mL) and the solution was cooled to −78° C. n-BuLi (2.5 M in hexanes, 1.5 mL, 3.75 mmol) was added drop wise and the mixture was stirred for 2 minutes at −78° C., then (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (800 mg, 3.7 mmol, Intermediate 11: step b) in 4 mL THF was introduced. After 5 minutes, the reaction mixture was transferred to a 0° C. bath. After 25 minutes, the reaction mixture was quenched with aqueous NH 4 Cl solution and the aqueous portion was extracted with EtOAc (3×50 mL). The combined organics were washed with brine, dried over MgSO 4 , filtered and concentrated. Chromatography on silica gel (2% MeOH-DCM increasing to 5% MeOH) provided the title compound as an off white solid. 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.11 (d, J=2.1 Hz, 1H), 7.75 (d, J=8.7 Hz, 1H), 7.30 (dd, J=8.7, 2.2 Hz, 1H), 6.99-6.84 (m, 2H), 4.61 (s, 1H), 4.09 (s, 3H), 3.92 (s, 3H), 3.81 (hept, J=7.1 Hz, 1H), 2.52 (s, 3H), 2.35 (s, 3H), 1.41-1.34 (d, J=7 Hz, 6H). MS (ESI): mass calcd. for Chemical Formula: C 24 H 26 ClN 5 O 2 , 451.2, m/z found, 452.1 [M+H] + . (4-Chloro-3-isopropyl-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified by chiral SFC (Stationary phase: Chiralpak AD 5 μm 250×30 mm, Mobile phase: 80% CO 2 , 20% MeOH) to give Example 132b as the first compound eluted from the chiral column and Example 132c as the second compound eluted from the chiral column.

Example 133a: (4-Chloro-3-isobutyl-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

To a flask containing 1-methyl-1H-1,2,3-triazole (250 mg, 3.01 mmol) was added THF (20 mL) and the solution was cooled to −45° C. using a CH 3 CN—CO 2 bath. n-BuLi (2.5 M in hexanes, 1.2 mL, 3 mmol) was added dropwise which produced an opaque suspension. The suspension was stirred at −45° C. for 20 minutes, then a homogeneous solution of (4-chloro-3-isobutyl-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)methanone (848 mg, 2.21 mmol, Intermediate 66: step d) in 3 mL THF was introduced at −45° C. After 10 minutes, the reaction vessel was placed in an ice-water bath. The reaction mixture was quenched after 40 minutes with aqueous NH 4 Cl solution. The aqueous portion was extracted with EtOAc (4×30 mL) and EtOAc:THF (1:1, 30 mL). The combined organics were washed with brine, dried over MgSO 4 , filtered and concentrated. Chromatography on silica gel (100% DCM increasing to 5% MeOH-DCM) provided the title compound as an off white amorphous solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.07 (d, J=2.2 Hz, 1H), 7.78 (d, J=8.7 Hz, 1H), 7.32 (dd, J=8.7, 2.2 Hz, 1H), 6.99-6.88 (m, 3H), 4.24 (s, 1H), 4.09 (s, 3H), 3.92 (s, 3H), 2.80 (d, J=7.3 Hz, 2H), 2.53 (s, 3H), 2.36 (s, 3H), 2.08 (hept, J=6.8 Hz, 1H), 0.95 (dd, J=6.7, 1.5 Hz, 6H). MS (ESI): mass calcd. for C 25 H 28 ClN 5 O 2 , 465.2: m/z found 466.0 [M+H] + . The racemate was separated by chiral chromatography using: Chiralpack OD-H column, 80% heptane/20% ethanol, to give Example 133b as the first compound eluted from the chiral column and Example 133c as the second compound eluted from the chiral column.

›Example 119a · 5 of 8

Example 134a: (4-Chloro-3-isobutyl-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

To a flask containing 1-methyl-1H-1,2,3-triazole (275 mg, 3.31 mmol) was added THF (10 mL) and the solution was cooled to −45° C. using a CH 3 CN—CO 2 bath. n-BuLi (2.5 M in hexanes, 1.4 mL, 3.5 mmol) was added dropwise which afforded an opaque white suspension. The mixture was stirred at −43° C. for 20 minutes, then a homogeneous solution of (4-chloro-3-isobutyl-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (550 mg, 1.48 mmol, Intermediate 65) in 3 mL THF was introduced. After 10 minutes, the reaction flask was then placed in an ice-water bath. The reaction mixture was quenched after 30 minutes with aqueous NH 4 Cl solution and the aqueous portion was extracted with EtOAc (4×30 mL) and EtOAc:THF (1:1, 30 mL). The combined organics were washed with brine, dried over MgSO 4 , filtered and concentrated. Chromatography on silica gel (5% MeOH-DCM increasing to 10% MeOH-DCM) provided the title compound as a tan amorphous solid. 1 H NMR (400 MHz, CD 3 OD) δ 8.19 (d, J=2.1 Hz, 1H), 7.85 (d, J=8.7 Hz, 1H), 7.52 (dd, J=8.7, 2.2 Hz, 1H), 7.17 (s, 1H), 6.11 (s, 1H), 4.09 (s, 3H), 3.98 (s, 3H), 3.47 (s, 3H), 2.85 (d, J=7.3 Hz, 2H), 2.37 (s, 3H), 2.11 (hept, J=6.9 Hz, 1H), 0.96 (d, J=6.6 Hz, 6H). MS (ESI): mass calcd. for C 23 H 27 ClN 6 O 2 , 454.2, m/z found 455.0 [M+H] + . The racemate was separated by chiral SFC (Stationary phase: CHIRALPAK AD-H 5 rpm, 250×30 mm, Mobile phase: 70% CO 2 , 30% mixture of MeOH/iPrOH 50/50 v/v (+0.3% iPrNH 2 )), to give Example 134b as the first compound that eluted from the chiral column and Example 134c as the second compound that eluted from the chiral column.

Example 135a: (4-Chloro-3-cyclopentyl-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

To a flask containing 1-methyl-1H-1,2,3-triazole (200 mg, 2.41 mmol) was added THF (15 mL) and the solution was cooled to −45° C. using a CH 3 CN—CO 2 bath. n-BuLi (2.5 M in hexanes, 1.0 mL, 2.5 mmol) was added dropwise which afforded an opaque white suspension. The mixture was stirred at −45° C. for 25 minutes, then a solution of (4-chloro-3-cyclopentyl-2-methoxyquinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (475 mg, 1.24 mmol, Intermediate 68: step c) in THF (3 mL) was introduced. After 10 minutes, the −45° C. bath was replaced with an ice-water bath. After 30 minutes, the reaction mixture was quenched with aqueous NH 4 Cl solution and extracted with EtOAc:THF (2:1, 3×40 mL). The combined organics were washed with brine, dried over MgSO 4 , filtered and concentrated. Chromatography on silica gel (3% MeOH-DCM increasing to 10% MeOH) provided the title compound as a light tan solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.24 (d, J=1.9 Hz, 1H), 7.69 (d, J=8.7 Hz, 1H), 7.35 (dd, J=8.7, 2.0 Hz, 2H), 7.07 (s, 1H), 6.01 (s, 1H), 4.07 (s, 3H), 3.90-3.80 (m, 4H containing a 3H singlet at 3.86), 3.32 (s, 3H), 2.06-1.94 (m, 3H), 1.94-1.84 (m, 6H), 1.75-1.69 (m, 2H). MS (ESI): mass calcd. for C 24 H 27 ClN 6 O 2 , 466.2: m/z found 467.1 [M+H] + . The racemate was separated by chiral SFC (Stationary phase: Chiralpak AD-H, 5 μm 250×20 mm, Mobile phase: 70% CO 2 : 30% EtOH (0.3% iPrNH 2 )) to give Example 135b as the first compound that eluted from the chiral column and Example 135c as the second compound that eluted from the chiral column.

Example 136a: (4-Chloro-3-cyclopentyl-2-methoxyquinolin-6-yl)(2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

To a flask containing 6-bromo-4-chloro-3-cyclopentyl-2-methoxyquinoline (Intermediate 68: step b, 500 mg, 1.47 mmol) was added THF (12 mL) and the solution was cooled to −78° C. n-BuLi (2.5 M in hexanes, 0.690 mL, 1.73 mmol) was added dropwise and the mixture was stirred for 3 minutes at −78° C. Then, (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (370 mg, 1.71 mmol, in 3 mL THF, Intermediate 11: step b) was introduced. The temperature was raised to 0° C. after 5 minutes. After 25 minutes, the reaction mixture was quenched with aqueous NH 4 Cl solution and the aqueous portion was extracted with EtOAc (3×50 mL). The combined organics were washed with brine, dried over MgSO 4 , filtered and concentrated. Chromatography on silica gel (2% MeOH-DCM increasing to 5% MeOH) provided the title compound as an off white amorphous solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.09 (s, 1H), 7.77 (d, J=8.7 Hz, 1H), 7.31 (d, J=8.7 Hz, 1H), 6.94 (m, 3H), 4.08 (s, 3H), 3.92 (s, 3H), 3.86 (m, 1H), 2.53 (s, 3H), 2.36 (s, 3H), 2.02-1.84 (m, 5H), 1.74-1.68 (m, 3H). MS (ESI): mass calcd. for C 26 H 28 ClN 5 O 2 , 477.2, m/z found 478.1 [M+H] + . The enantiomers were separated by chiral SFC (Stationary phase: Chiralpak AD 5 μm 250×30 mm), Mobile phase: 70% CO 2 , 30% MeOH (0.3% iPrNH 2 ) to give Example 136b as the first compound eluted from the chiral column and Example 136c as the second compound eluted from the chiral column.

Example 137: (4-Chloro-2-methoxy-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

To a flask containing 2,4-dichloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol (210 mg, 0.43 mmol, Example 160) was added MeOH (10 mL) followed by solid sodium methoxide (30 mg, 0.54 mmol, 97% purity) at room temperature. The reaction mixture was heated to 50° C. for 15 hours, then cooled to room temperature and concentrated. The crude material was chromatographed directly on silica gel (2% MeOH-DCM increasing to 8% MeOH) which provided a mixture of regioisomeric products. RP-HPLC (acetonitrile/water+0.05% TFA) provided the title compound as an off white solid. 1 H NMR (500 MHz, CD 3 OD) δ ppm 8.32 (d, J=1.9 Hz, 1H), 7.96 (d, J=8.8 Hz, 1H), 7.60 (dd, J=8.8, 2.1 Hz, 1H), 7.44 (s, 1H), 6.87 (s, 1H), 4.14 (s, 3H), 4.02-3.89 (m, 5H), 3.69 (s, 3H), 2.65 (s, 3H). MS (ESI): mass calc. for C 21 H 20 ClF 3 N 6 O 2 , 480.1; found, 481.1 (M+H) + .

›Example 119a · 6 of 8

Example 138: (2-Chloro-4-methoxy-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

To a flask containing 1-methyl-1H-1,2,3-triazole (160 mg, 1.93 mmol) was added THF (12 mL) and the colorless solution was cooled to −43° C. using a CH 3 CN—CO 2 bath. Then, n-BuLi, (2.5 M in hexanes, 0.72 mL, 1.8 mmol) was added which afforded an opaque white suspension. The suspension was stirred at −40° C. for 20 minutes, then a homogeneous solution of (2-chloro-4-methoxy-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1,2-dimethyl-1H-imidazol-5-yl)methanone (350 mg, 0.88 mmol, Intermediate 59: step c) in 5 mL THF, was introduced at −40° C. The reaction mixture was allowed to warm gradually to 0° C. over 25 minutes, then quenched with aqueous NH 4 Cl solution. The aqueous portion was extracted with EtOAc (3×35 mL). The combined organics were washed with brine, dried over Na 2 SO 4 and MgSO 4 , filtered and concentrated to give a brown solid. Chromatography on silica gel (3% MeOH-DCM increasing to 10% MeOH) provided the title compound as a tan amorphous solid. 1 H NMR (500 MHz, CD 3 OD) δ ppm 8.22 (d, J=1.8 Hz, 1H), 8.05 (d, J=8.9 Hz, 1H), 7.70 (dd, J=8.9, 2.0 Hz, 1H), 7.48 (s, 1H), 6.91 (s, 1H), 4.09 (s, 3H), 4.03-3.89 (m, 5H), 3.69 (s, 3H), 2.66 (s, 3H). MS (ESI): mass calcd. for C 21 H 20 ClF 3 N 6 O 2 , 480.1, found, 481.1 (M+H) + .

Example 139a: [2-Azetidin-1-yl-4-chloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

(2,4-Dichloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanol (150 mg, 0.28 mmol, Example 161), azetidine (56.7 μL, 0.841 mmol), and DMF (7 mL) were combined in a reaction tube, then sealed and heated to 100° C. and maintained at that temperature overnight. The reaction vessel was then cooled to room temperature and contents were transferred to a separatory funnel with EtOAc dilution, then extracted three times with deionized water. The organic phase was separated, dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude material was purified via reverse phase chromatography using acetonitrile with ammonium hydroxide in water as eluent to provide the title compound. MS (ESI): mass calcd. for C 25 H 20 ClF 6 N 5 O, 555.1; m/z found, 556.6 [M+H] + . 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.79 (d, J=2.2 Hz, 1H), 8.06 (d, J=2.2 Hz, 1H), 7.85 (dd, J=8.3, 2.2 Hz, 1H), 7.64 (d, J=8.8 Hz, 1H), 7.60 (d, J=8.2 Hz, 1H), 7.44 (dd, J=8.8, 2.2 Hz, 1H), 7.04 (s, 1H), 6.15 (d, J=1.3 Hz, 1H), 4.38-4.29 (m, 4H), 3.86-3.75 (m, 2H), 3.28 (s, 3H), 2.42-2.33 (m, 2H).

[2-Azetidin-1-yl-4-chloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl) pyridin-3-yl]methanol was purified via SFC with a Chiralpak AD-H column (5 μm 250×20 mm) using a mobile phase of 80% CO 2 and a 20% methanol to provide two enantiomers. The first eluting enantiomer was Example 139b: MS (ESI): mass calcd. for C 25 H 20 ClF 6 N 5 O, 555.1; m/z found, 556 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.79 (d, J=2.2 Hz, 1H), 8.05 (d, J=2.1 Hz, 1H), 7.86 (dd, J=8.3, 2.2 Hz, 1H), 7.69-7.59 (m, 2H), 7.45 (dd, J=8.8, 2.2 Hz, 1H), 7.14 (s, 1H), 6.20 (s, 1H), 4.34 (t, J=7.5 Hz, 4H), 3.81 (q, J=9.8 Hz, 2H), 3.30 (s, 3H), 2.42-2.34 (m, 2H) and the second eluting enantiomer was Example 139c: MS (ESI): mass calcd. for C 25 H 20 ClF 6 N 5 O, 555.1; m/z found, 556.2 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.78 (d, J=2.3 Hz, 1H), 8.06 (d, J=2.2 Hz, 1H), 7.86 (dd, J=8.2, 2.2 Hz, 1H), 7.68-7.59 (m, 2H), 7.45 (dd, J=8.8, 2.2 Hz, 1H), 7.11 (s, 1H), 6.17 (s, 1H), 4.34 (t, J=7.5 Hz, 4H), 3.80 (q, J=9.8 Hz, 2H), 3.29 (s, 3H), 2.38 (p, J=7.4 Hz, 2H).

Example 140a: [4-Azetidin-1-yl-2-chloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

Purification of the crude reaction mixture from the synthesis of [2-azetidin-1-yl-4-chloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]-methanol (Example 139a) also provided the title compound as a regioisomer. MS (ESI): mass calcd. for C 25 H 20 ClF 6 N 5 O, 555.1; m/z found, 556.6 [M+H] + . 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.77 (d, J=2.2 Hz, 1H), 7.84 (dd, J=8.2, 2.2 Hz, 1H), 7.81-7.76 (m, 1H), 7.69 (d, J=8.8 Hz, 1H), 7.65 (d, J=8.2 Hz, 1H), 7.54 (dd, J=8.9, 2.0 Hz, 1H), 7.07 (s, 1H), 6.13 (d, J=1.3 Hz, 1H), 4.47-4.36 (m, 4H), 3.95-3.82 (m, 2H), 3.30 (s, 3H), 2.43-2.34 (m, 2H).

[4-Azetidin-1-yl-2-chloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol was purified via SFC with a Chiralpak AD-H column (5 m 250×20 mm) using a mobile phase of 70% CO 2 and a 30% methanol to provide two enantiomers. The first eluting enantiomer was Example 140b: MS (ESI): mass calcd. for C 25 H 20 ClF 6 N 5 O, 555.1; m/z found, 556.2 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.79-8.74 (m, 1H), 7.84 (dd, J=8.1, 2.2 Hz, 1H), 7.80 (d, J=2.0 Hz, 1H), 7.69 (d, J=8.8 Hz, 1H), 7.66 (d, J=8.2 Hz, 1H), 7.54 (dd, J=8.8, 2.0 Hz, 1H), 7.11 (s, 1H), 6.14 (s, 1H), 4.47-4.37 (m, 4H), 3.94-3.82 (m, 2H), 3.31 (s, 3H), 2.43-2.32 (m, 2H) and the second eluting enantiomer was Example 140c: MS (ESI): mass calcd. for C 25 H 20 ClF 6 N 5 O, 555.1; m/z found, 556 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.77 (d, J=2.2 Hz, 1H), 7.86 (dd, J=8.2, 2.2 Hz, 1H), 7.82-7.78 (m, 1H), 7.74 (d, J=8.7 Hz, 1H), 7.67 (d, J=8.2 Hz, 1H), 7.55 (dd, J=8.9, 2.0 Hz, 1H), 7.18 (s, 1H), 6.21 (s, 1H), 4.48-4.37 (m, 4H), 3.95-3.83 (m, 2H), 3.32 (s, 3H), 2.44-2.35 (m, 2H).

Example 141: [2,4-Dichloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

6-Bromo-2,4-dichloro-3-(2,2,2-trifluoroethyl)quinoline (1.00 g, 2.79 mmol, Intermediate 69: step d), (2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanone (0.602 g, 2.79 mmol, Intermediate 11: step b) were dissolved in THF (30 mL) in a dry round bottom flask under an N 2 atmosphere, then cooled to −40° C. in dry ice acetone bath. n-BuLi (1.6 M in hexanes, 1.74 mL, 2.79 mmol) was then added dropwise via syringe over approximately 2 minutes. The contents were stirred at −40° C. for approximately 45 minutes, then the dry ice bath was removed and replaced with an ice water bath and the reaction was stirred at that temperature for approximately 45 minutes. The reaction was quenched with a saturated, aqueous NH 4 Cl, then transferred to a separatory funnel with EtOAc. The organic phase was separated, then the aqueous layer was back extracted twice with EtOAc, then the combined organic phases were dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude product was purified by flash column chromatography (silica gel, 0-50% DCM/(10% of a 2 M NH 3 MeOH in DCM)) to afford the title compound. MS (ESI): mass calcd. for C 22 H 18 Cl 2 F 3 N 5 O, 495.1; m/z found, 496.3 [M+H] + . 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.27 (dd, J=2.1, 0.6 Hz, 1H), 8.03 (dd, J=8.8, 0.6 Hz, 1H), 7.60 (dd, J=8.9, 2.1 Hz, 1H), 6.97 (d, J=8.1 Hz, 1H), 6.93 (d, J=8.1 Hz, 1H), 6.88 (s, 1H), 4.13-4.01 (m, 2H), 3.94 (s, 3H), 2.55 (s, 3H), 2.38 (s, 3H).

›Example 119a · 7 of 8

Example 142a: [2-Azetidin-1-yl-4-chloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

[2,4-Dichloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(l-methyl-1H-1,2,3-triazol-5-yl)methanol (200 mg, 0.28 mmol, Example 141), azetidine (82 μL, 1.2 mmol), and DMF (2 mL) were combined in a reaction tube, then sealed and heated to 100° C. and maintained at that temperature overnight. The reaction vessel was then cooled to room temperature and contents were transferred to a separatory funnel with EtOAc dilution, then extracted three times with deionized water. The organic phase was separated, dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude material was purified via reverse phase chromatography using acetonitrile with ammonium hydroxide in water as eluent to provide the title compound. MS (ESI): mass calcd. for C 25 H 24 ClF 3 N 6 O, 516.2; m/z found, 517.5 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.00 (d, J=2.2 Hz, 1H), 7.64 (d, J=8.8 Hz, 1H), 7.30 (dd, J=8.8, 2.2 Hz, 1H), 6.96 (d, J=8.1 Hz, 1H), 6.90 (d, J=8.1 Hz, 1H), 6.83 (s, 1H), 4.35 (t, J=7.5 Hz, 4H), 3.88 (s, 3H), 3.84-3.76 (m, 2H), 2.49 (s, 3H), 2.43-2.35 (m, 2H), 2.31 (s, 3H).

[2-Azetidin-1-yl-4-chloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified via SFC with a Chiralpak AD-H column (5 m 250×20 mm) using a mobile phase of 75% CO 2 and a 25% methanol to provide two enantiomers. The first eluting enantiomer was Example 142b MS (ESI): mass calcd. for C 25 H 24 ClF 3 N 6 O, 516.2; m/z found, 517.6 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 7.99 (d, J=2.2 Hz, 1H), 7.64 (d, J=8.8 Hz, 1H), 7.29 (dd, J=8.8, 2.2 Hz, 1H), 6.97 (d, J=8.0 Hz, 1H), 6.91 (d, J=8.1 Hz, 1H), 6.86 (s, 1H), 4.39-4.32 (m, 4H), 3.90 (s, 3H), 3.84-3.75 (m, 2H), 2.50 (s, 3H), 2.43-2.36 (m, 2H), 2.33 (s, 3H) and the second eluting enantiomer was Example 142c: MS (ESI): mass calcd. for C 25 H 24 ClF 3 N 6 O, 516.2; m/z found, 517.6 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 7.99 (d, J=2.2 Hz, 1H), 7.64 (d, J=8.8 Hz, 1H), 7.29 (dd, J=8.8, 2.2 Hz, 1H), 6.97 (d, J=8.1 Hz, 1H), 6.92 (d, J=8.1 Hz, 1H), 6.86 (s, 1H), 4.39-4.32 (m, 4H), 3.90 (s, 3H), 3.84-3.76 (m, 2H), 2.51 (s, 3H), 2.43-2.35 (m, 2H), 2.33 (s, 3H).

Example 143a: [4-Chloro-2-methoxy-3-(2,2,2-trifluoroethyl)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol

[2,4-Dichloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(l-methyl-1H-1,2,3-triazol-5-yl)methanol (100 mg, 0.202 mmol, Example 141), toluene (2 mL), and sodium methoxide (109 mg, 2.02 mmol) were combined in a round bottom flask equipped with a stirbar and condenser under an N 2 atmosphere. The reaction solution was heated to 60° C. and maintained at that temperature for 4 hours. The reaction was cooled to room temperature and contents were transferred to a separatory funnel with EtOAc dilution and extracted with a saturated, aqueous NH 4 Cl solution. The aqueous layer was separated, neutralized with 10% aqueous HCl, then extracted twice with ethyl acetate. The combined organic phases were dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude material was purified via reverse phase chromatography using acetonitrile with ammonium hydroxide in water as eluent to afford the title compound. MS (ESI): mass calcd. for C 23 H 21 ClF 3 N 5 O 2 , 491.1; m/z found, 492.5 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.19 (d, J=2.2 Hz, 1H), 7.78 (d, J=8.7 Hz, 1H), 7.39 (dd, J=8.7, 2.2 Hz, 1H), 6.96-6.89 (m, 2H), 6.74 (s, 1H), 4.11 (s, 3H), 3.91 (s, 3H), 3.83-3.74 (m, 2H), 2.47 (s, 3H), 2.29 (s, 3H). [4-Chloro-2-methoxy-3-(2,2,2-trifluoroethyl)quinolin-6-yl](2,6-dimethylpyridin-3-yl)(1-methyl-1H-1,2,3-triazol-5-yl)methanol was purified via SFC with a Chiralpak AD-H column (5 μm 250×20 mm) using a mobile phase of 82% CO 2 and an 18% mixture of (MeOH/iPrOH 50/50 v/v (+0.3% iPrNH 2 )) to provide two enantiomers. The first eluting enantiomer was Example 143b: MS (ESI): mass calcd. for C 23 H 21 ClF 3 N 5 O 2 , 491.1; m/z found, 492.1 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ 8.19 (d, J=2.1 Hz, 1H), 7.76 (d, J=8.7 Hz, 1H), 7.36 (dd, J=8.8, 2.2 Hz, 1H), 6.94 (s, 2H), 6.76 (s, 1H), 4.11 (s, 3H), 3.95 (s, 3H), 3.83-3.71 (m, 2H), 2.51 (s, 3H), 2.32 (s, 3H) and the second eluting enantiomer was Example 143c: MS (ESI): mass calcd. for C 23 H 21 ClF 3 N 5 O 2 , 491.1; m/z found, 492.1 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.19 (d, J=2.1 Hz, 1H), 7.76 (d, J=8.7 Hz, 1H), 7.36 (dd, J=8.8, 2.2 Hz, 1H), 6.94 (s, 2H), 6.76 (s, 1H), 4.11 (s, 3H), 3.95 (s, 3H), 3.83-3.71 (m, 2H), 2.51 (s, 3H), 2.32 (s, 3H).

Example 144: [2,4-Dichloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

5-Bromo-1,2-dimethyl-1H-imidazole (359 mg, 1.85 mmol) and THF (10 mL) were combined in a dry round bottom flask under an N 2 atmosphere and cooled to −78° C. in a dry ice acetone bath. n-BuLi (1.6 M in hexanes, 1.74 mL, 2.79 mmol) was then added dropwise via syringe over approximately 2 minutes and the contents were allowed to stir at −78° C. for an additional 10 minutes. 2,4-Dichloro-3-(2,2,2-trifluoroethyl)quinoline-6-carboxylate (250 mg, 0.74 mmol, Intermediate 71) in THF (5 mL) was then cannulated into the reaction vessel and the reaction was stirred at −78° C. for 10 minutes. The dry ice bath was removed and replaced by an ice water bath and the reaction continued for approximately 30 minutes. The reaction was quenched with a saturated, aqueous NH 4 Cl solution, then transferred to a separatory funnel with EtOAc. The organic phase was separated and the aqueous layer was back extracted with EtOAc, then the combined organic phases were dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude material was purified via reverse phase chromatography using acetonitrile with ammonium hydroxide in water as eluent. The fractions from the purification containing the desired product were transferred to a separatory funnel with EtOAc and extracted with a saturated, aqueous NaHCO 3 solution. The aqueous layer was separated, extracted with EtOAc, then the combined organic phases were dried over MgSO 4 , filtered and concentrated under reduced pressure to afford the title compound. MS (ESI): mass calcd. for C 22 H 20 Cl 2 F 3 N 5 O, 497.1; m/z found, 498.1 [M+H] + ; 1 H NMR (400 MHz, CDCl 3 ) δ ppm 8.38 (s, 1H), 7.90-7.81 (m, 1H), 7.66 (d, J=9.0 Hz, 1H), 6.02 (s, 2H), 4.12-3.99 (m, 2H), 3.41 (d, J=2.6 Hz, 6H), 2.24 (d, J=2.9 Hz, 6H).

›Example 119a · 8 of 8

Example 145: [4-Chloro-2-methoxy-3-(2,2,2-trifluoroethyl)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol

[2,4-Dichloro-3-(2,2,2-trifluoroethyl)quinolin-6-yl][bis(1,2-dimethyl-1H-imidazol-5-yl)]methanol (150 mg, 0.202 mmol, Example 144), toluene (2 mL), and sodium methoxide (163 mg, 3.01 mmol) were combined in a round bottom flask equipped with a stirbar and condenser under an N 2 atmosphere. The reaction solution was heated to 60° C. and maintained at that temperature for 4 hours. The reaction was then cooled to room temperature and the contents were transferred to a separatory funnel with EtOAc dilution, then extracted with a saturated, aqueous NH 4 Cl solution. The aqueous layer was separated, neutralized then extracted twice with ethyl acetate. The combined organic layers were then dried over MgSO 4 , filtered and concentrated under reduced pressure. The crude material was purified via reverse phase chromatography using acetonitrile with ammonium hydroxide in water as eluent to provide the title compound. MS (ESI): mass calcd. for C 23 H 23 ClF 3 N 5 O 2 , 493.1; m/z found, 494.2 [M+H] + ; 1 H NMR (600 MHz, CDCl 3 ) δ ppm 8.35-8.22 (m, 1H), 7.68 (d, J=8.7 Hz, 1H), 7.51-7.43 (m, 1H), 6.04 (s, 2H), 4.12 (s, 3H), 3.81 (q, J=10.0 Hz, 2H), 3.41 (s, 6H), 2.25 (s, 6H).

Example 146: (4-Chloro-2-methoxy-3-{[4-(trifluoromethyl)piperidin-1-yl]carbonyl}quinolin-6-yl)(1-methyl-1H-imidazol-5-yl)[6-(trifluoromethyl)pyridin-3-yl]methanol

To a solution of (4-chloro-6-iodo-2-methoxyquinolin-3-yl)(4-(trifluoromethyl)piperidin-1-yl)methanone (125 mg, 0.25 mmol, Intermediate 35: step f) and (1-methyl-1H-imidazol-5-yl)(6-(trifluoromethyl)pyridin-3-yl)methanone (64 mg, 0.25 mmol, Intermediate 10: step c) in THF (6.9 mL) at −78° C. was added n-BuLi (1.85 M in hexanes, 136 μL, 0.25 mmol) dropwise. The resulting dark yellow solution was stirred at −78° C. for 15 minutes, then warmed to 0° C. and stirred for an additional 30 minutes. Saturated aqueous NH 4 Cl (8 mL), water (20 mL) and EtOAc (20 mL) were added and the layers separated. The aqueous layer was further extracted with EtOAc (20 mL). The organics were combined, dried (Na 2 SO 4 ), filtered and concentrated to dryness to afford a yellow oil. The crude material was purified by FCC (0.5-7.5% MeOH/DCM) followed by reverse-phase HPLC (acetonitrile/water+NH 4 OH) to provide the title compound as a white solid. 1 H NMR (500 MHz, CDCl 3 ) δ ppm 8.82-8.78 (m, 1H), 8.25-8.06 (m, 1H), 7.96-7.91 (m, 1H), 7.88-7.82 (m, 1H), 7.71-7.66 (m, 1H), 7.66-7.56 (m, 1H), 7.45-7.36 (m, 1H), 6.44 (s, 1H), 4.98-4.90 (m, 1H), 4.31-4.11 (m, 1H), 4.11-4.07 (m, 3H), 3.56-3.48 (m, 1H), 3.40-3.35 (m, 3H), 3.14-3.04 (m, 1H), 2.90-2.82 (m, 1H), 2.38-2.25 (m, 1H), 2.11-2.02 (m, 1H), 1.88-1.78 (m, 1H), 1.75-1.65 (m, 1H). MS (ESI): mass calcd. for C 28 H 24 ClF 6 N 5 O 3 , 627.1; m/z found, 628.0 [M+H] + .

Example 147: 4-Chloro-N-(cyclopropylmethyl)-6-[(2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl]-2-methoxyquinoline-3-carboxamide

A mixture of 4-chloro-6-((2,6-dimethylpyridin-3-yl)(hydroxy)(1-methyl-1H-1,2,3-triazol-5-yl)methyl)-2-methoxyquinoline-3-carboxylic acid (177 mg, 0.35 mmol, Intermediate 37), EDCI (103 mg, 0.53 mmol) and HOBt (72 mg, 0.53 mmol) in DMF (3.9 mL) was stirred at room temperature for 15 minutes. Then, cyclopropanemethylamine (154 μL, 1.72 mmol) was added and the reaction mixture stirred at room temperature for 1 hour. The mixture was concentrated to dryness and the residue pa

›Tables in the description — 2

A 1 is C (1-2) alkyl;	A 2 is C (1-2) alkyl, CH 2 CH 2 OCH 3 , or OCH 3 ; or A 1 and A 2 may be taken together with their attached nitrogen to form a ring which is:
R a is H, OH, OCH 3 , F;	R 8 is H, CH 3 , OCH 3 , or F;	R 9 is H;

TABLE 1 — ***% inhibition is shown at 0.67 μM compound concentration. Compounds marked “ND” were not tested.

		RORγt	RORγt		RORγt
		(LBD)	(LBD)		(FL)	Human
		Reporter	Reporter	RORγt (FL)	Reporter	Th17
	ThermoFluor ®	Assay,	Assay, %	Reporter	Assay, %	Assay,
Example	Assay, Kd	IC50	inhibition	Assay,	inhibition	IC 50
Number	(μM)	(μM)	@ 6 μM	IC50 (μM)	@ 6 μM	(μM)
1a	ND	ND	ND	ND	ND	ND
1b	0.22	1.8	78	ND	ND	ND
1c	0.0001	~2	66	ND	ND	ND
2a	ND	ND	ND	ND	ND	ND
2b	ND	ND	ND	ND	ND	ND
2c	0.11	ND	ND	ND	ND	ND
3	0.1	1.8	81	ND	ND	ND
4a	0.016	>6	24	ND	ND	ND
4b	0.009	>6	42	ND	ND	>6
4c	0.053	>6	−6	ND	ND	ND
5a	0.25	>6	45	ND	ND	ND
5b	0.35	>6	33	ND	ND	ND
5c	0.13	>6	36	ND	ND	ND
6a	0.22	>6	26	ND	ND	ND
6b	0.24	>6	17	ND	ND	ND
6c	0.13	>6	26	ND	ND	ND
7a	0.22	0.71	80	ND	ND	ND
7b	0.06	0.45	84	ND	ND	0.21
7c	0.49	>6	47	ND	ND	ND
8	0.031	0.11	93	ND	ND	0.2
9	8.2	>6	16	ND	ND	ND
10	0.52	~6	55	ND	ND	ND
11a	0.39	1.5	86	ND	ND	ND
11b	0.14	0.56	93	ND	ND	ND
11c	3.6	5	52	ND	ND	ND
12a	0.29	1.1	93	ND	ND	ND
12b	9.6	>6	23	ND	ND	ND
12c	0.063	0.33	93	ND	ND	ND
13a	0.025	0.25	100	ND	ND	ND
13b	1.1	~4	70	ND	ND	ND
13c	0.029	0.042	100	ND	ND	0.065
14a	0.0025	0.023	99	ND	ND	ND
14b	1.4	4.5	72	ND	ND	ND
14c	0.0013	0.03	98	ND	ND	ND
15a	0.029	0.08	94	ND	ND	ND
15b	0.0051	~0.04	97	ND	ND	ND
15c	0.15	0.7	86	ND	ND	ND
16	0.029	ND	ND	0.12	101	ND
17a	ND	ND	ND	ND	ND	ND
17b	0.0059	ND	ND	0.071	105	ND
17c	0.017	ND	ND	0.095	107	ND
18	0.35	1.7	80	ND	ND	ND
19	0.45	0.37	95	ND	ND	1.3
20	3.8	1.3	87	ND	ND	ND
21	1.1	1.1	93	ND	ND	ND
22	3.1	~6	50	ND	ND	ND
23	4.3	~6	55	ND	ND	ND
24	2	>6	29	ND	ND	ND
25	7.4	~4	73	ND	ND	ND
26	0.48	~1	100	ND	ND	ND
27	6.6	0.42	100	ND	ND	~3
28	4.8	~6	54	ND	ND	ND
29	0.026	~0.4	66	ND	ND	ND
30	0.1	0.23	92	ND	ND	ND
31	0.027	>6	−32	ND	ND	ND
32	0.039	>6	32	ND	ND	ND
33	4.8	>6	−32	ND	ND	ND
34a	0.084	>6	10	ND	ND	ND
34b	0.03	ND	ND	0.72	39	ND
34c	0.089	ND	ND	0.81	50	ND
35	1.3	1.8	77	ND	ND	ND
36a	0.1	0.56	68	ND	ND	ND
36b	6.9	>6	24	ND	ND	ND
36c	0.026	0.77	77	ND	ND	ND
37a	0.37	~6	47	ND	ND	ND
37c	0.16	1	54	ND	ND	ND
38a	0.0088	0.064	99	ND	ND	ND
38b	4.7	~6	60	ND	ND	ND
38c	0.02	0.026	100	ND	ND	ND
39	0.2	1.1	90	ND	ND	ND
40	0.3	~0.9	79	ND	ND	ND
41	0.091	0.095	97	ND	ND	ND
42	0.088	0.19	98	ND	ND	ND
43	0.022	0.024	98	ND	ND	ND
44	0.19	0.37	93	ND	ND	ND
45	0.25	~2	83	ND	ND	ND
46	0.38	1.7	85	ND	ND	ND
47	0.75	~3	75	ND	ND	ND
48	0.19	1	96	ND	ND	ND
49a	0.48	1.2	93	ND	ND	ND
49b	0.25	0.71	94	ND	ND	ND
49c	2.5	>6	48	ND	ND	ND
50a	0.17	0.74	68	ND	ND	ND
50b	6.1	ND	ND	1.8	55	ND
50c	0.24	ND	ND	0.35	90	ND
51a	ND	ND	ND	ND	ND	ND
51b	3	ND	ND	>6	4	ND
51c	5	>6	−4	>6	13	ND
52	2.9	ND	ND	>6	10	ND
53a	0.19	0.83	57	ND	ND	ND
53b	0.14	ND	ND	0.64	49	ND
53c	0.07	ND	ND	0.45	74	ND
54	0.86	~6	53	2.3	73	ND
55a	0.83	~3	68	ND	ND	ND
55b	0.48	~5	57	1.6	62	ND
55c	0.4	1	52	1.8	69	ND
56a	0.037	0.21	94	ND	ND	ND
56b	0.0099	0.19	86	0.12	99	0.012
56c	0.02	0.42	92	0.18	89	ND
57a	0.014	0.11	98	ND	ND	ND
57b	0.0082	0.17	99	0.12	97	ND
57c	0.0063	0.34	97	0.088	96	ND
58	0.72	2.1	89	1.3	85	ND
59	0.071	0.91	81	0.62	75	ND
60	0.47	~1	78	ND	ND	ND
61	0.0088	0.32	93	ND	ND	ND
62	1.1	2.2	83	ND	ND	ND
63	0.46	1.5	81	ND	ND	ND
64	0.025	0.18	95	ND	ND	ND
65a	ND	ND	ND	ND	ND	ND
65b	5.3	>6	39	ND	ND	ND
66a	0.63	>6	−112	ND	ND	ND
66b	0.26	0.32	−129	ND	ND	ND
67a	ND	ND	ND	ND	ND	ND
67b	1	>6	−37	ND	ND	ND
68a	0.011	0.087	83	ND	ND	ND
68b	1.2	5	54	ND	ND	ND
68c	0.0038	0.02	87	ND	ND	ND
69a	0.058	~6	57	ND	ND	ND
69b	4.6	>6	37	ND	ND	ND
69c	0.014	0.19	61	ND	ND	ND
70a	3.3	>6	36	ND	ND	ND
70b	2.2	>6	28	ND	ND	ND
71a	0.48	~0.2	86	ND	ND	ND
71c	0.11	0.23	87	ND	ND	ND
72a	0.041	0.057	85	ND	ND	ND
72b	3.3	~6	53	ND	ND	ND
72c	0.0082	0.033	86	ND	ND	ND
73a	ND	ND	ND	ND	ND	ND
73b	8.5	>6	−15	ND	ND	ND
73c	0.031	>6	−80	ND	ND	ND
74a	ND	ND	ND	ND	ND	ND
74b	4.9	>6	−9	ND	ND	ND
75	0.009	>6	−1	ND	ND	ND
76a	ND	ND	ND	ND	ND	ND
76b	1.2	>6	−17	ND	ND	ND
76c	0.11	>6	−35	ND	ND	ND
77a	ND	ND	ND	ND	ND	ND
77b	0.084	0.073	100	ND	ND	ND
78a	ND	ND	ND	ND	ND	ND
78b	0.19	3.4	56	ND	ND	ND
78c	1.9	~4	59	ND	ND	ND
79a	ND	ND	ND	ND	ND	ND
79b	1.2	>6	30	ND	ND	ND
79c	0.2	>6	−2.5	ND	ND	ND
80a	0.016	0.026	99	ND	ND	ND
80b	0.019	0.041	100	0.021	104	0.019
80c	0.048	0.099	99	ND	ND	ND
81a	ND	ND	ND	ND	ND	ND
81b	2.2	3.2	57	ND	ND	ND
81c	0.0061	0.029	102	ND	ND	0.01
82a	0.0058	0.019	101	ND	ND	ND
82b	0.52	3.7	91	ND	ND	ND
82c	0.0021	0.014	104	ND	ND	0.0012
83	0.36	ND	ND	ND	ND	ND
84a	0.016	0.047	94	ND	ND	ND
84b	0.011	0.096	97	ND	ND	0.0054
84c	0.19	0.97	87	ND	ND	ND
85a	ND	ND	ND	ND	ND	ND
85b	0.22	~0.8	88	ND	ND	ND
85c	0.0013	0.00099, ~0.008	99	ND	ND	0.0016
86a	ND	ND	ND	ND	ND	ND
86b	0.95	~2	83	ND	ND	ND
86c	0.025	0.13	97	ND	ND	0.021
87a	ND	ND	ND	ND	ND	ND
87b	0.22	1	92	ND	ND	ND
87c	0.016	0.23	87	ND	ND	0.12
88a	ND	ND	ND	ND	ND	ND
88b	0.21	1	82	ND	ND	ND
88c	0.027	0.42	75	ND	ND	0.31
89a	ND	ND	ND	ND	ND	ND
89b	0.43	0.6	95	ND	ND	ND
89c	0.022	0.051	99	ND	ND	0.079
90a	0.2	0.46	101	ND	ND	ND
90b	1.7	2.8	70	ND	ND	ND
90c	0.043	0.2	96	ND	ND	0.2
91a	ND	ND	ND	ND	ND	ND
91b	1.6	1.8	82	ND	ND	ND
91c	0.13	0.44	100	ND	ND	0.52
92a	0.35	0.78	87	ND	ND	ND
92b	4	1.8	50	ND	ND	ND
92c	0.2	0.64	96	ND	ND	ND
93a	ND	ND	ND	ND	ND	ND
93b	1.2	~6	48	ND	ND	ND
93c	0.064	0.45	100	ND	ND	ND
94a	ND	ND	ND	ND	ND	ND
94b	3.7	4.5	68	ND	ND	ND
94c	0.08	0.22	97	ND	ND	0.38
95a	ND	ND	ND	ND	ND	ND
95b	0.023	0.19	97	ND	ND	0.061
95c	0.18	0.9	93	ND	ND	ND
96	0.69	1.4	80	ND	ND	ND
97	0.75	1.6	68	ND	ND	ND
98	0.036	0.22	97	ND	ND	ND
99a	ND	ND	ND	ND	ND	ND
99b	0.065	0.19	96	ND	ND	1.2
99c	1.8	~4	67	ND	ND	ND
100a	ND	ND	ND	ND	ND	ND
100b	0.5	0.75	90	ND	ND	ND
100c	0.00031	0.012	100	ND	ND	0.0076
101a	0.27	0.73	84	ND	ND	ND
101b	1.6	~4	73	ND	ND	ND
101c	0.038	0.29	89	ND	ND	0.36
102a	0.23	0.62	78	ND	ND	ND
102b	1.7	~1	70	ND	ND	ND
102c	0.24	0.22	90	ND	ND	ND
103a	0.0011	0.0093	101	ND	ND	ND
103b	0.51	0.89	90	ND	ND	ND
103c	0.0015	0.022	99	ND	ND	ND
104a	ND	ND	ND	ND	ND	ND
104b	0.087	0.69	85	ND	ND	ND
104c	0.25	0.96	84	ND	ND	ND
105a	ND	ND	ND	ND	ND	ND
105b	17	>6	33	ND	ND	ND
105c	0.047	0.12	87	ND	ND	ND
106a	ND	ND	ND	ND	ND	ND
106b	0.0023	0.017	103	0.018	103	0.012
106c	0.0053	0.017	100	ND	ND	0.0014
107a	0.0057	0.019	54	ND	ND	ND
107b	2	>6	38	ND	ND	ND
107c	0.0057	0.019	54	ND	ND	ND
108a	ND	ND	ND	ND	ND	ND
108b	20	>6	6	ND	ND	ND
108c	0.046	>6	14	ND	ND	ND
109a	ND	ND	ND	ND	ND	ND
109b	6.9	>6	28	ND	ND	ND
109c	0.02	0.018	79	ND	ND	ND
110a	ND	ND	ND	ND	ND	ND
110b	11	>6	4	ND	ND	ND
110c	0.048	0.3	76	ND	ND	ND
111	0.0024	0.12	97	ND	ND	0.017
112a	ND	ND	ND	ND	ND	ND
112b	0.054	0.17	97	ND	ND	ND
112c	0.003	0.061	96	ND	ND	0.017
113a	ND	ND	ND	ND	ND	ND
113b	0.88	0.72	77	ND	ND	ND
114a	ND	ND	ND	ND	ND	ND
114b	1.4	~2	60	ND	ND	ND
114c	0.0039	0.041	72	ND	ND	ND
115	1	1.7	69	ND	ND	ND
116a	0.11	0.21	97	0.091	87*	0.21
116b	0.0085	0.047	98	0.037	95*	0.03
116c	0.11	0.21	97	0.091	87*	0.21
117a	ND	ND	ND	ND	ND	ND
117b	0.4	ND	ND	ND	ND	ND
117c	0.00002	ND	ND	ND	ND	ND
118	0.004	ND	ND	0.33	86	0.22
119a	ND	ND	ND	ND	ND	ND
119b	0.0022	ND	ND	0.039	86	ND
119c	0.0063	ND	ND	0.065	93	ND
120a	ND	ND	ND	ND	ND	ND
120b	0.13	ND	ND	ND	ND	ND
120c	0.014	ND	ND	ND	ND	ND
121a	ND	ND	ND	ND	ND	ND
121b	0.93	ND	ND	ND	ND	ND
121c	0.0017	ND	ND	ND	ND	ND
122a	ND	ND	ND	ND	ND	ND
122b	0.0008	ND	ND	0.023	98	ND
122c	0.0083	ND	ND	0.038	100	ND
123a	ND	ND	ND	ND	ND	ND
123b	0.018	ND	ND	0.13	95	ND
123c	0.019	ND	ND	0.17	95	ND
124a	ND	ND	ND	ND	ND	ND
124b	0.0024	ND	ND	0.12	87	>6
124c	0.0048	ND	ND	0.31	87	0.035
125a	ND	ND	ND	ND	ND	ND
125b	0.032	ND	ND	0.084	86*	ND
125c	0.00031	ND	ND	0.026	94	ND
126a	ND	ND	ND	ND	ND	ND
126b	0.00047	ND	ND	0.014	101*	ND
126c	0.63	ND	ND	0.37	78*	ND
127a	ND	ND	ND	ND	ND	ND
127b	0.01	ND	ND	0.032	100	ND
127c	0.14	ND	ND	0.28	91	ND
128a	ND	ND	ND	ND	ND	ND
128b	0.24	ND	ND	0.23	89*	ND
128c	0.022	ND	ND	0.053	87*	ND
129a	ND	ND	ND	ND	ND	ND
129b	0.84	ND	ND	0.9	88	ND
129c	0.0013	ND	ND	0.012	100*	ND
130a	ND	ND	ND	ND	ND	ND
130b	0.15	ND	ND	0.35	82*	ND
130c	0.00059	ND	ND	0.025	87	ND
131a	ND	ND	ND	ND	ND	ND
131b	0.087	ND	ND	0.14	81*	ND
131c	0.0033	ND	ND	0.05	94*	ND
132a	0.027	0.12	101	ND	ND	ND
132b	1.4	~5	70	ND	ND	ND
132c	0.0041	0.058	99	ND	ND	ND
133a	0.0012	0.011	98	ND	ND	ND
133b	0.41	0.84	90	ND	ND	ND
133c	0.0011	0.0064	98	ND	ND	ND
134a	0.00058	0.015	101	ND	ND	ND
134b	0.00054	0.0024	101	ND	ND	ND
134c	0.0014	0.0031	99	ND	ND	ND
135a	0.00066	0.0082	100	ND	ND	ND
135b	0.00075	ND	ND	ND	ND	ND
135c	0.0012	ND	ND	ND	ND	ND
136a	0.00095	0.06	97	ND	ND	ND
136b	0.71	2.3	76	0.95	79	ND
136c	0.0014	0.014	98	0.011	95*	ND
137	0.0013	ND	ND	0.011	105	ND
138	0.38	ND	ND	0.75	96	ND
139a	0.079	0.3	101	ND	ND	ND
139b	0.48	0.96	99	ND	ND	ND
139c	0.043	0.17	101	ND	ND	ND
140a	1.7	~6	48	ND	ND	ND
140b	16	>6	48	ND	ND	ND
140c	5.5	~6	56	ND	ND	ND
141	0.038	0.21	99	ND	ND	ND
142a	0.13	0.14	100	ND	ND	ND
142b	2.4	~6	53	ND	ND	ND
142c	0.018	0.061	101	ND	ND	ND
143a	ND	ND	ND	ND	ND	ND
143b	1.2	3	77	ND	ND	ND
143c	0.0029	0.045	100	ND	ND	0.37
144	0.012	0.21	101	ND	ND	ND
145	0.0009	ND	ND	0.074	103	ND
146	0.85	ND	ND	1.4	79	ND
147	0.93	ND	ND	ND	ND	ND
148	1.7	ND	ND	ND	ND	ND
149	6.8	ND	ND	2.5	42	ND
150	3.4	ND	ND	2.5	57	ND
151	5.1	ND	ND	>6	45	ND
152	0.6	ND	ND	ND	ND	ND
153	3	ND	ND	ND	ND	ND
154a	0.075	>6	35	ND	ND	ND
154b	4.2	>6	30	ND	ND	ND
154c	0.019	3.6	45	ND	ND	ND
155	1.4	5.4	64	ND	ND	ND
156	3.4	>6	34	ND	ND	ND
157	0.28	1.3	68	ND	ND	ND
158	0.17	1.7	81	ND	ND	ND
159	0.16	1	86	ND	ND	ND
160	ND	ND	ND	ND	ND	ND
161	ND	ND	ND	ND	ND	ND
162	ND	ND	ND	ND	ND	ND
163	ND	ND	ND	ND	ND	ND
164	ND	ND	ND	ND	ND	ND
165	ND	ND	ND	ND	ND	ND
166a	ND	ND	ND	ND	ND	ND
166b	0.61	0.36	96	ND	ND	ND
166c	1.5	2.2	84	ND	ND	ND
167	ND	ND	ND	ND	ND	ND
168	ND	ND	ND	ND	ND	ND
169	ND	ND	ND	ND	ND	ND
170	ND	ND	ND	ND	ND	ND
171	ND	ND	ND	ND	ND	ND
172	12	>6	12	ND	ND	ND
173	ND	ND	ND	ND	ND	ND
174	ND	ND	ND	ND	ND	ND
175	ND	ND	ND	ND	ND	ND
176	ND	ND	ND	ND	ND	ND
177	ND	ND	ND	ND	ND	ND
178	ND	ND	ND	ND	ND	ND
179	ND	ND	ND	ND	ND	ND
180	ND	ND	ND	ND	ND	ND
181	ND	ND	ND	ND	ND	ND
182a	0.029	ND	ND	0.12	101	ND
182b	2.5	ND	ND	1.5	79	ND
182c	0.012	ND	ND	0.081	100	ND
183	0.015	ND	ND	0.95	33	ND
184	0.017	ND	ND	1	53	ND
185	0.77	ND	ND	>6	6	ND
186	0.59	ND	ND	>6	5	ND
187	0.54	ND	ND	0.64	72*	ND
188a	ND	ND	ND	ND	ND	ND
188b	0.0036	ND	ND	0.041	98*	ND
188c	3.4	ND	ND	0.84	42*	ND
189a	ND	ND	ND	ND	ND	ND
189b	0.051	ND	ND	0.17	102	ND
189c	49	ND	ND	>6	16	ND
190a	ND	ND	ND	ND	ND	ND
190b	0.58	ND	ND	0.75	38*	ND
190c	0.0012	ND	ND	0.021	84*	ND
191a	ND	ND	ND	ND	ND	ND
191b	0.098	ND	ND	0.096	93*	ND
191c	0.0031	ND	ND	0.033	95	ND
192a	ND	ND	ND	ND	ND	ND
192b	0.27	ND	ND	0.36	97	ND
192c	0.004	ND	ND	0.022	99*	ND
193	0.042	ND	ND	1.1	82	ND
194	0.11	ND	ND	2	61	ND
195	0.026	ND	ND	1.4	79	ND
196	0.0012	ND	ND	0.28	104	ND
197	0.0027	ND	ND	0.23	75	ND
198	0.0012	ND	ND	0.12	96	ND
199	0.0096	ND	ND	0.19	86	ND
200	0.01	ND	ND	0.17	79	ND
201	0.0025	ND	ND	0.91	94	0.27
202	ND	ND	ND	0.13	68*	ND
203	0.0078	ND	ND	0.62	86*	ND
204	ND	ND	ND	0.35	83	ND
205a	ND	ND	ND	ND	ND	ND
205b	0.41	ND	ND	0.56	58*	ND
205c	0.00048	ND	ND	0.021	99*	ND
206a	0.0044	ND	ND	0.041	103	ND
206b	0.0046	ND	ND	0.018	102	ND
206c	0.01	ND	ND	0.047	100	ND
207a	0.076	ND	ND	0.058	92	ND
207b	0.076	ND	ND	0.44	67**	ND
207c	0.015	ND	ND	0.05	97*	ND
208a	ND	ND	ND	ND	ND	ND
208b	0.014	ND	ND	0.071	88*	ND
208c	0.27	ND	ND	0.28	84*	ND
209a	ND	ND	ND	ND	ND	ND
209b	2.8	ND	ND	1.1	25*	ND
209c	0.0086	ND	ND	0.045	96*	ND
210a	ND	ND	ND	ND	ND	ND
210b	3.6	ND	ND	0.97	33*	ND
210c	0.0076	ND	ND	0.059	101*	ND
211a	ND	ND	ND	ND	ND	ND
211b	0.72	ND	ND	0.68	56*	ND
211c	0.13	ND	ND	0.28	87*	ND
212a	ND	ND	ND	ND	ND	ND
212b	0.38	ND	ND	0.69	53*	ND
212c	0.065	ND	ND	0.37	77*	ND
213a	ND	ND	ND	ND	ND	ND
213b	1.2	ND	ND	1	31*	ND
213c	0.003	ND	ND	0.013	95*	ND
214a	ND	ND	ND	ND	ND	ND
214b	0.23	ND	ND	0.62	48*	ND
214c	0.021	ND	ND	0.11	55*	ND
215a	ND	ND	ND	ND	ND	ND
215b	0.26	ND	ND	0.51	65*	ND
215c	0.059	ND	ND	0.046	90*	ND
216a	0.23	ND	ND	0.41	77*	ND
216b	2.5	ND	ND	0.92	27*	ND
216c	0.11	ND	ND	0.12	82*	ND
217a	ND	ND	ND	ND	ND	ND
217b	1.3	ND	ND	>2	13*	ND
217c	0.0096	ND	ND	0.026	63*	ND
218a	ND	ND	ND	ND	ND	ND
218b	0.73	ND	ND	0.86	53*	ND
218c	0.0032	ND	ND	0.018	104*	ND
219a	ND	ND	ND	ND	ND	ND
219b	1	ND	ND	1.3	55	ND
219c	1.2	ND	ND	1.8	47	ND
220	0.00006	ND	ND	0.014	103*	ND
221	0.0033	ND	ND	0.12	104	ND
222	0.31	ND	ND	0.46	50	ND
223a	ND	ND	ND	ND	ND	ND
223b	0.048	ND	ND	0.14	95	ND
223c	0.05	ND	ND	0.22	96	0.19
224a	ND	ND	ND	ND	ND	ND
224b	0.67	ND	ND	0.58	64*	ND
224c	0.046	ND	ND	0.13	97*	ND
225a	ND	ND	ND	ND	ND	ND
225b	0.18	ND	ND	0.67	66*	ND
225c	0.002	ND	ND	0.008	99*	ND
226a	ND	ND	ND	ND	ND	ND
226b	0.69	ND	ND	0.56	58*	ND
226c	0.0033	ND	ND	0.018	102*	ND
227a	ND	ND	ND	ND	ND	ND
227b	0.075	ND	ND	0.42	79*	0.052
227c	0.0024	ND	ND	0.015	100*	ND
228	ND	ND	ND	ND	ND	ND
229	ND	ND	ND	ND	ND	ND
230	ND	ND	ND	ND	ND	ND
231	ND	ND	ND	ND	ND	ND
232	ND	ND	ND	ND	ND	ND
233	ND	ND	ND	ND	ND	ND
234	ND	ND	ND	ND	ND	ND
235	ND	ND	ND	ND	ND	ND
236	ND	ND	ND	ND	ND	ND
237	ND	ND	ND	ND	ND	ND
238a	>24	>6	−8	ND	ND	ND
238b	13	>6	9	ND	ND	ND
239	>62	ND	ND	2.1	24	ND
240a	0.036	ND	ND	0.11	91*	ND
240b	0.15	ND	ND	0.32	88*	ND
240c	0.024	ND	ND	0.045	93*	ND
241a	ND	ND	ND	ND	ND	ND
241b	0.024	ND	ND	0.07	94*	ND
241c	0.44	ND	ND	0.52	74*	ND
242a	ND	ND	ND	ND	ND	ND
242b	0.43	ND	ND	0.9	48*	ND
242c	0.052	ND	ND	0.095	99*	ND
243a	0.01	ND	ND	0.074	69*	ND
243b	0.055	ND	ND	0.17	66***	ND
243c	0.0031	ND	ND	0.061	76*	ND
244a	0.0024	ND	ND	0.018	83*	ND
244b	9.3	ND	ND	0.78	42*	ND
244c	0.0035	ND	ND	0.012	86*	ND
245a	0.028	ND	ND	0.073	90*	ND
245b	0.85	ND	ND	0.66	39*	ND
245c	0.013	ND	ND	0.045	93*	ND
246	ND	ND	ND	ND	ND	ND
247	ND	ND	ND	ND	ND	ND
248	0.00046	ND	ND	0.024	106	ND
All data shown in Table 1 is either the value of one data point or the average of more than one data point. In cases where more than one value is shown in a table cell, values with qualifiers such as ~, > or < could not be included in the averaging calculation for the value shown on the left side of the table cell.
*% inhibition is shown at 2 μM compound concentration,
**% inhibition is shown at 1 μM compound concentration,

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18 claims

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14 codes

IPC · International Patent Classification

Section A — Human necessities

A61K31/506
A61K31/5365
A61K45/06
A61K31/5377
A61K31/4709
A61K31/4985
A61K31/496

Section C — Chemistry; metallurgy

C07D405/14
C07D487/04
C07D417/14
C07D409/14
C07D401/06
C07D413/14
C07D401/14

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Nizal S Chandrakumar

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