Inverted microscope

The present application is a National Phase entry of PCT Application No. PCT/EP2011/000932, filed Feb. 25, 2010, which claims priority from German Application No 102010014501.7, filed Apr. 10, 2010, the disclosures of which are hereby incorporated by reference herein in their entirety.

The invention relates to an inverted microscope, comprising a microscope stand having a stand housing, composed of a rear wall, a front wall and side walls, and an objective turret, a focusing drive, and a rotatable retaining device, which is designed as a reflector turret and which has a plurality of locations for receiving exchangeable optical components.

In inverted microscopes, focusing drive, reflector unit, and objective turret are arranged one above the other.

The reflector unit can be designed as a turret or a slider and can usually be removed for changing components. With more than three optical components (reflector modules), one turret variety is usually chosen. The diameter of the turret is determined by the number of reflector modules it can accommodate. At a certain number, the diameter of the turret or the parts required for operation exceeds the width of the stand, so it must be arranged entirely above the focusing drive knobs in order to avoid a collision.

The known microscopes with inverted design have change mechanisms for optical components in the area between objective and tube lens which can be designed as turret or slider, e.g., for the projection of the reflected-light beam path. They can be exchangeable or stationary.

In addition, optical components such as magnification changers (Optovars) and contrast elements (analyzers), etc., can be housed in these turrets or sliders instead of these projections.

Generally, the turrets in these inverted microscopes are, similar to the optical axis, centrally arranged in the microscope and have a vertical turret axis of rotation.

In DE 102004034887, an inverted microscope having a holding apparatus, insertable reversibly into the microscope for interchangeable optical components is described. The holding apparatus is embodied as a pivoting drawer that is arranged pivotably in and out about a pivot axis. During exchange or insertion of optical components, the pivoting drawer always remains connected to the microscope stand even in the pivoted-out state.

The solutions in the prior art are fundamentally disadvantageous because the arrangement of a change mechanism is contingent upon a relatively tall design height of the microscope. This is particularly undesirable for small inverted microscopes used primarily for routine operations because a low stage height is a very important ergonomic criterion. Operating effortlessly on the microscope stage while simultaneously resting the arm on the work table is not always ensured.

The height of the microscope stage is therefore set at a minimum when using a reflector turret as a change mechanism for optical components and an ergonomic drive height. This is predetermined by the necessary arrangement of the drive knobs (distance between eyepiece and drive knob) in the area of the reflector turret with inverted stands. In order to avoid collision with the drive knobs, the reflector turret must be arranged above them. Reflector sliders are also conceivable but they have substantial disadvantages due to the limited number of positions, e.g., for the use with many different fluorescence applications.

Moreover, the solutions of the prior art are disadvantageous because the turret design and the slider design have to be at least partially removed from the microscope stand when changing components of the change mechanism.

Therefore, the invention addresses the problem of further developing the design of an inverted microscope such that the disadvantages of the prior art are largely eliminated, allowing for a simpler and quicker changing of optical components for various applications.

This problem is solved, according to the invention, with an inverted microscope of the initially described type wherein an axis of rotation of the retaining device is arranged parallel to an optical axis of the microscope with the axis or rotation having a displacement (Vx) from the optical axis in the direction toward a first one of the side walls of the stand housing and a displacement (Vy) from the optical axis in the direction toward the front wall of the stand housing, wherein the retaining device protrudes from the first one of the side walls by a distance (Ax) through an opening, and the receiving locations are located in the retaining device such that optical components can be pivoted into the optical axis of the microscope by rotating the retaining device.

According to the invention, the axis of rotation of the retaining device runs parallel to the optical axis with a displacement in the direction of a side wall of the stand housing, wherein the retaining device protrudes from said side wall of the stand housing.

It is advantageous for the retaining device to protrude from a side wall because it does not have to be removed from the stand housing when components are changed, allowing thus for a simpler and quicker change of components.

The locations for receiving optical components are introduced in the retaining device such that they can be pivoted into the optical axis of the microscope by rotating the retaining device.

In an example embodiment, the retaining device has four locations for receiving optical components, wherein it is also conceivable to increase or decrease said number.

Depending on the use of the microscope, the rotation of the retaining device can be performed manually or with the use of a motorized drive, e.g., a tooth belt drive.

It is advantageous, for example, to arrange the motorized drive unit in the installation space of the stand housing, which is facing away from the lateral displacement of the axis of rotation of the retaining device in the direction of one side wall of the stand housing, because said displacement provides for sufficient space in this area.

Advantageously, the focusing drive, having a coarse and a fine focus, is arranged below the retaining device in the side walls of the stand housing at a height ergonomic for the operator, wherein the drive knob for the coarse and fine focus is expediently located in the side wall of the stand housing which is facing away from the lateral displacement of the axis of rotation of the retaining device.

A drive knob with a smaller diameter, e.g., which only affects the fine focus, can be arranged on the side of the stand housing with the protruding retaining device.

It is also conceivable that the smaller drive knob for fine focusing can be omitted.

Due to the arrangement of the retaining device for optical components designed as reflector turret in conjunction with the arrangement of the focusing drive, the retaining device and the object stage can structurally be lowered greatly. Free access to the drive knobs for the coarse and fine focus is assured despite the lowered retaining device.

In a further possible embodiment, the lowering of the object stage can be foregone, using only the simple change option of the optical components resulting from the lateral displacement.

In the following, the inverted microscope, according to the invention, is further described in terms of two embodiments:

FIG. 1 depicts a schematic depiction of the inverted microscope as seen from the operator;

FIG. 2 depicts a cross-sectional view A-A according to FIG. 1 ;

FIG. 3 depicts a perspective partial depiction of the microscope; and

FIG. 4 depicts a further perspective partial depiction of the microscope without the lowering of the object stage.

In a first embodiment, FIG. 1 shows the inverted microscope, according to the invention, with an object stage 1 , an objective turret 2 , a retaining device 3 designed as a reflector turret for exchangeable optical components ( FIG. 2 ), and a focusing drive including a coarse focus 4 with drive knob 5 and a fine focus 6 with drive knob 7 , which are attached to a microscope stand 8 or to a stand housing with a rear wall 9 , a left and a right side wall 10 and 11 and a front wall 12 ( FIG. 3 ).

The retaining device 3 is arranged on the microscope stand 8 such that its axis of rotation 13 runs parallel to the optical axis 14 of the microscope with a displacement Vx in the direction of the side wall 10 of the stand housing and a displacement Vy ( FIG. 2 ) in the direction of the front wall 12 ( FIG. 3 ) of the stand housing.

For the purpose of rotating the retaining device 3 , it is coupled with a motorized drive unit 15 (only schematically depicted), which is arranged in the installation space of the stand housing ( FIG. 2 ).

The retaining device 3 has four receiving locations 16 , 17 , 18 , and 19 ( FIG. 2 ), wherein FIG. 1 only shows receiving location 17 .

For the purpose of placing the optical components 20 , 21 , 22 , and 23 in the retaining device 3 , it protrudes from the side wall 10 of the stand housing by a distance Ax. The side wall 10 has an opening, which according to FIGS. 3 and 4 , is covered with a removable cover flap 24 .

FIG. 2 shows the cross-sectional view A-A denoted in FIG. 1 with the receiving locations 16 , 17 , 18 , and 19 for the optical components 20 , 21 , 22 , and 23 .

The receiving locations 16 , 17 , 18 , and 19 are introduced in the retaining device 3 such that they can be pivoted into the optical axis of the microscope through its rotation.

FIG. 3 schematically shows the retaining device 3 protruding from the side wall 10 in its closed state. For the purpose of attaching the optical components 20 , 21 , 22 , and 23 to the retaining device 3 , the cover flap 24 is removed. FIG. 3 also shows the arrangement of the fine focus 6 with the drive knob 7 .

In a second embodiment, FIG. 4 also shows schematically the retaining device 3 protruding from the side wall 10 in its closed state, wherein the cover flap 24 has four receiving surfaces 25 for denoting the optical components 20 , 21 , 22 , and 23 . Since the lowering of the object stage is foregone in this embodiment, the microscope has a further drive knob 26 in addition to the coarse and fine focus 4 with the drive knob 5 as shown in FIGS. 1 and 2 . The fine focus 6 with the drive knob 7 , according to FIGS. 1 and 2 , can be omitted.

1 Object stage

2 Objective turret

3 Retaining device (reflector turret)

4 Coarse focus

5 , 7 , 26 Drive knob

6 Fine focus

8 Microscope stand

9 Rear wall

10 , 11 Side wall

12 Front wall

13 Axis of rotation

14 Optical axis

16 , 17 , 18 , 19 Motorized drive unit

16 , 17 , 18 , 19 Receiving location

20 , 21 , 22 , 23 Optical component

24 Cover flap

25 Receiving surface

Vx, Vy Displacement

Ax Distance