Herein is reported a method for the purification of an antibody directly captured from clarified cell culture supernatants using Streamline CST and/or Capto MMC, wherein especially product related (aggregates and fragments) and process related impurities (host cell protein, media components) could efficiently be removed, resulting in a preparation with a purity comparable to classical protein A affinity chromatography.

Claims

11 · 1 independent · depth 4

11 granted claims

›1.independentA method for producing an anti-IGF-1R antibody comprising the following steps:na) applying a crude mammalian cell culture cultivation supernatant to a…+10

›2.↳ 1The method according to claim 1, wherein the method comprises the following additional step a-1) prior to step a):na-1) applying a buffered solution c…d2+1

›3.↳ 1The method according to claim 1 wherein the method comprises the following additional step a-b) after step a) and prior to step b):na-b) applying a bu…d2+4

›6.↳ 1The method according to claim 1 wherein the inorganic salt is selected from sodium, chloride, potassium chloride, and ammonium chloride.d2

›7.↳ 1The method according to claim 1 wherein the buffered solution in step b) comprises 20 mM to 30 mM Tris, 1050 mM to 1350 mM sodium chloride, and about …d2

›11.↳ 1The method according to claim 1 wherein the multimodal weak cation exchange chromatography material comprises cross-linked agarose to which a carboxyl…d2

Classifications

3 codes

IPC · International Patent Classification

Section C — Chemistry; metallurgy

C07K1/18
C07K16/06
C07K1/16

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Pendency

4.1 y

1,497 days filing → grant

Office actions

non-final + final

Responses

no RCE

Examiner

James Rogers

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Citations: 13 back · 0 forward

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1 priority documents

›Priority documents — 1

Type	Document	Date
related publication	US 20130217866 A1	22 Aug 2013

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