The present invention relates to novel bacterial and mycobacterial compositions containing RNA and cell walls, and methods for making and using these compositions. These compositions have immune stimulating and anti-cancer activity.

Claims

36 · 4 independent · depth 3

36 granted claims

›1.independentA method of making a composition comprising isolated mycobacterial RNA associated with mycobacterial cell wall fragments, comprising the sequential st…+12

›2.↳ 1The method of claim 1, wherein the isolated mycobacterial RNA associated with mycobacterial cell wall fragments is in the form of oligoribonucleotides…d2

›3.↳ 1The method of claim 1, further comprising after step A, optional step B or after step C a step of heating a composition obtained in a previous step at…d2+1

›4.↳ 1The method of claim 1, wherein the disrupting step A comprises high pressure homogenization at about 10,000 psi or higher.d2

›5.↳ 1The method of claim 1, wherein the disrupting step A is repeated 2 to 5 times before the separation steps B or C.d2

›6.↳ 1The method of claim 1, further comprising repeating the disrupting step A 2 to 5 times after the separation step C.d2

›7.↳ 1The method of claim 1, wherein the separating step B is low speed centrifugation and the separating step C is high speed centrifugation.d2

›8.↳ 1The method of claim 1, further comprising:nrepeating step A and optionally step B to control the number of the intact mycobacterial cells; and,after s…d2+1

›16.↳ 1The method of claim 1, wherein the mycobacterial cell is selected from the group consisting of Mycobacterium phlei, Mycobacterium vaccae, Mycobacteriu…d2

›17.↳ 1The method of claim 1, wherein the mycobacterial cell is Mycobacterium phlei.d2

›18.↳ 1The method of claim 1, wherein the isolated mycobacterial RNA associated with bacterial cell wall fragments is in the form of oligoribonucleotides and…d2

›9.independentA method of making a composition comprising isolated bacterial RNA associated with bacterial cell wall fragments comprising the sequential steps of:nA…+6

›10.↳ 9The method of claim 9, wherein the isolated bacterial RNA associated with bacterial cell wall fragments are from gram positive bacteria or gram negati…d2

›11.↳ 9The method of claim 9, wherein the isolated bacterial RNA associated with bacterial cell wall fragments is in the form of oligoribonucleotides and pol…d2

›12.↳ 9The method of claim 9, wherein the isolated bacterial RNA associated with bacterial cell wall fragments is in the form of oligoribonucleotides and pol…d2

›13.↳ 9The method of claim 9, further comprising after step A, optional step B or step C a step of heating a composition obtained in a previous step at a tem…d2+2

›21.independentA method of making a composition comprising isolated mycobacterial RNA associated with mycobacterial cell wall fragments, comprising the steps of:nA) …+10

›22.↳ 21The method of claim 21, wherein the intact mycobacterial cells are inactivated during the step of heating.d2

›23.↳ 21The method of claim 21, wherein the composition does not contain phenol or Streptomyces griseus protease.d2

›24.↳ 21The method of claim 21, wherein the mycobacterial cell is selected from the group consisting of Mycobacterium phlei, Mycobacterium vaccae, Mycobacteri…d2

›25.↳ 21The method of claim 21, wherein the mycobacterial cell is Mycobacterium phlei.d2

›26.↳ 21The method of claim 21, wherein the disrupting step A comprises high pressure homogenization at about 10,000 psi or higher.d2

›27.↳ 21The method of claim 21, wherein the disrupting step A is repeated 2 to 5 times before the separation steps B or C.d2

›28.↳ 21The method of claim 21, further comprising repeating the disrupting step A 2 to 5 times after the separation step C.d2

›29.↳ 21The method of claim 21, wherein the separating step B is low speed centrifugation and the separating step C is high speed centrifugation.d2

›30.↳ 21The method of claim 21, further comprising repeating step A and optionally step B to control the number of the intact mycobacterial cells.d2

›31.↳ 21The method of claim 21, wherein the temperature is sufficient to generate oligoribonucleotides and polyribonucleotides of about 2 to about 40 bases in…d2

›32.independentA method of making a composition comprising isolated bacterial RNA associated with bacterial cell wall fragments comprising the steps of:nA) disruptin…+4

Classifications

6 codes

IPC · International Patent Classification

Section A — Human necessities

A61K31/7105
A61K39/00
A61K35/74
A61K39/39
A61K39/04

Section C — Chemistry; metallurgy

C12N1/06

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Pendency

1.6 y

574 days filing → grant

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Examiner

Brian J Gangle

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Priority chain

2 priority documents

Priority

13 Oct 2010

earliest claimed

›Priority documents — 2

Type	Document	Date
provisional	US 61392498	13 Oct 2010
related publication	US 20140322372 A1	30 Oct 2014

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