A polypeptide solution of the present invention is a polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent. The solvent contains at least one selected from the following (i)-(iii): (i) DMSO; (ii) DMSO with an inorganic salt; and (iii) DMF with an inorganic salt. Further, in the present invention, an artificial polypeptide fiber is obtained by: using the polypeptide solution as a dope solution; and extruding the dope solution from a spinneret into a desolvation bath so as to eliminate the solvent from the dope solution and form a fiber to produce an undrawn yarn. Moreover, in the present invention, a polypeptide is purified by subjecting the polypeptide solution to heat treatment and thereafter removing an undissolved substance therefrom. Thus, the present invention provides the polypeptide solution whose solute has high solubility and solvent itself is low cost, and that allows dissolution at high temperatures and has high safety: a method for producing an artificial polypeptide fiber: and a method for purifying a polypeptide.

Claims

17 · 1 independent · depth 3

17 granted claims

›1.independentA polypeptide solution in which a polypeptide derived from natural spider silk proteins is dissolved in a solvent,nwherein the solvent contains at lea…+16

›2.↳ 1The polypeptide solution according to claim 1, wherein, when the polypeptide solution is 100 mass %, the percentage of the polypeptide is in a range o…d2

›3.↳ 1The polypeptide solution according to claim 1, wherein, when the solvent is 100 mass %, the percentage of the inorganic salt is in a range of 0.1 to 2…d2

›4.↳ 1The polypeptide solution according to claim 1, wherein, when the solvent is 100 mass %, the percentage of the at least one substance selected from (i)…d2

›5.↳ 1The polypeptide solution according to claim 1, wherein, when the solvent is 100 mass %, the percentage of the at least one substance selected from (i)…d2

›6.↳ 1The polypeptide solution according to claim 1, wherein the inorganic salt is at least one selected from alkali metal halides, alkaline-earth metal hal…d2

›7.↳ 1The polypeptide solution according to claim 1, wherein the polypeptide solution is a dope solution.d2

›8.↳ 1A method for producing an artificial polypeptide fiber using the polypeptide solution according to claim 1, comprising:nusing the polypeptide solution…d2+3

›12.↳ 1A method for purifying a polypeptide using the polypeptide solution according to claim 1, comprising:nsubjecting the polypeptide solution to heat trea…d2+5

Classifications

13 codes

IPC · International Patent Classification

Section B — Performing operations; transporting

B29D99/00

Section C — Chemistry; metallurgy

C08K5/20
C08K3/16
C07K14/435
C08K5/41

Section D — Textiles; paper

D01D5/04
D01F4/02

USPC · US Patent Classification

106/124.4530/418530/344264/177.19264/202530/858

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2.5 y

918 days filing → grant

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none on record

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Examiner

David M Brunsman

art unit 1732 · TC 1700

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1 priority documents

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Type	Document	Date
related publication	US 20140245923 A1	4 Sep 2014

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