A method for extracting and storing nucleic acids from a sample, comprising:nproviding the sample on a dry non-dissolvable solid matrix comprising;a protein denaturant; andan acid or acid-titrated buffer reagent;generating an acidic pH upon hydration for extraction of nucleic acids from the sample, where the acidic pH is in a range from 3 to 6;drying the matrix comprising the extracted nucleic acids; andstoring the extracted nucleic acids on the matrix in a substantially dry state at ambient temperature, wherein the matrix comprises cellulose, cellulose acetate, glass fiber or combinations thereof.
›23.↳ 22The method of claim 22 further comprising recovering the nucleic acids from the matrix.d2+2
The method of claim 22 further comprising recovering the nucleic acids from the matrix.
›24.↳ 23The method of claim 23, wherein the recovery of the nucleic acids from the matrix comprises rehydrating the matrix in an aqueous solution, a buffer or…d3
The method of claim 23, wherein the recovery of the nucleic acids from the matrix comprises rehydrating the matrix in an aqueous solution, a buffer or an organic solution.
›25.↳ 23The method of claim 23, wherein the recovery of the nucleic acids from the matrix comprises electroelution.d3
The method of claim 23, wherein the recovery of the nucleic acids from the matrix comprises electroelution.
›26.↳ 22The method of claim 22, wherein the sample is a biological sample.d2+2
The method of claim 22, wherein the sample is a biological sample.
›27.↳ 26The method of claim 26, wherein the biological sample comprises blood, serum, tissue, saliva, or cells.d3
The method of claim 26, wherein the biological sample comprises blood, serum, tissue, saliva, or cells.
›28.↳ 26The method of claim 26, wherein the sample is a cell extract, a tissue culture cell preparation, an impure nucleic acidor a purified nucleic acid.d3
The method of claim 26, wherein the sample is a cell extract, a tissue culture cell preparation, an impure nucleic acidor a purified nucleic acid.
›29.↳ 22The method of claim 22, wherein the extracted nucleic acids comprise RNA, DNA or a combination thereof.d2
The method of claim 22, wherein the extracted nucleic acids comprise RNA, DNA or a combination thereof.
›30.↳ 22The method of claim 22, wherein the extracted nucleic acids comprise RNA.d2+1
The method of claim 22, wherein the extracted nucleic acids comprise RNA.
›31.↳ 30The method of claim 30, wherein the extracted RNA has a RIN of at least 4.d3
The method of claim 30, wherein the extracted RNA has a RIN of at least 4.
›32.↳ 22The method of claim 22, wherein the matrix further comprises a UV protectant, a free-radical scavenger, a chelator or combinations thereof.d2+1
The method of claim 22, wherein the matrix further comprises a UV protectant, a free-radical scavenger, a chelator or combinations thereof.
›33.↳ 32The method of claim 32, wherein the UV protectant or free-radical scavenger is selected from the group consisting of MEHQ, HQ, THQ, and ascorbic acid.d3
The method of claim 32, wherein the UV protectant or free-radical scavenger is selected from the group consisting of MEHQ, HQ, THQ, and ascorbic acid.
›34.↳ 22The method of claim 22, wherein the matrix further comprises an RNase inhibitor.d2
The method of claim 22, wherein the matrix further comprises an RNase inhibitor.
›35.↳ 22The method of claim 22, wherein the matrix comprises a porous cellulose paper.d2
The method of claim 22, wherein the matrix comprises a porous cellulose paper.
›36.↳ 22The method of claim 22, wherein the protein denaturant is selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, so…d2
The method of claim 22, wherein the protein denaturant is selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, potassium thiocyanate, arginine, SDS, urea and combinations thereof.
›37.↳ 22The method of claim 22, wherein the matrix further comprises a reducing agent is selected from the group consisting of DTT, 2-ME, TCEP, TCEP-HCl and a…d2
The method of claim 22, wherein the matrix further comprises a reducing agent is selected from the group consisting of DTT, 2-ME, TCEP, TCEP-HCl and a combination thereof.
›38.↳ 22The method of claim 22, wherein the acid comprises acetic acid, citric acid, tartaric acid, phosphoric acid, hydrochloric acid, TCEP-HCl , TCEP-O-HCl …d2
The method of claim 22, wherein the acid comprises acetic acid, citric acid, tartaric acid, phosphoric acid, hydrochloric acid, TCEP-HCl , TCEP-O-HCl , sulfuric acid, nitric acid, vanillic acid, 3-(N-morpholino)propanesulfonic acid or combinations thereof
