The present disclosure generally relates to dry solid matrices for the extraction, stabilization, and storage of nucleic acids, particularly RNA, in a dry format under ambient conditions for a prolonged period of time. Methods for collecting and recovering the nucleic acids stored in the dry solid matrix are also described.

Claims

23 · 2 independent · depth 4

23 granted claims

›1.independentA dry solid matrix for extraction and storage of nucleic acids from a biological sample, wherein a composition comprising;na metal thiocyanate compris…+13

›2.↳ 1The dry solid matrix of claim 1, wherein the Group 1 or Group 2 metal cation is selected from the group consisting of Na+, K+, Li+, Mg+2, Ca+2, and Ba…d2

›3.↳ 1The dry solid matrix of claim 1, wherein the composition present in the solid matrix further comprises a UV inhibitor, a free-radical trap, a chelator…d2+1

›4.↳ 1The dry solid matrix of claim 1, wherein the composition incorporated into the dry solid matrix further comprises an RNase inhibitor.d2+2

›5.↳ 1The dry solid matrix of claim 1, wherein the dry solid matrix permits prolonged storage of nucleic acids in a dry format under ambient conditions.d2+2

›8.↳ 1The dry solid matrix of claim 1, wherein the porous matrix is a cellulose paper.d2

›9.↳ 1The dry solid matrix of claim 1, wherein the metal thiocyanate is selected from the group consisting of sodium thiocyanate, potassium thiocyanate, lit…d2

›10.↳ 1The dry solid matrix of claim 1, wherein the buffer is selected from the group consisting of 2-amino-2-hydroxymethyl-propane-1,3-diol (Tris), 2-(N-mor…d2+1

›15.independentA method for extracting and storing nucleic acids from a sample comprising:na) providing a dry solid matrix for extraction and storage of nucleic acid…+8

›16.↳ 15The method of claim 15, wherein the metal cation is selected from the group consisting of Na+, K+, Li+, Mg+2, Ca+2, and Ba+2.d2

›17.↳ 15The method of claim 15, wherein the method further comprises recovering the nucleic acids from the solid matrix.d2+2

›18.↳ 15The method of claim 15, wherein the biological sample is blood, serum, tissue, saliva, nasal mucous, or cells.d2

›19.↳ 15The method of claim 15, wherein the sample is a purified nucleic acid sample or a tissue culture cell preparation.d2

›20.↳ 15The method of claim 15, wherein purification of the nucleic acids from the sample is not required prior to applying the sample to the dry solid matrix…d2

›21.↳ 15The method of claim 15, wherein the method permits the prolonged storage of RNA in a dry format under ambient conditions.d2

Classifications

6 codes

IPC · International Patent Classification

Section B — Performing operations; transporting

B01J20/04
B01J20/24

Section C — Chemistry; metallurgy

C12N15/10

Section G — Physics

G01N1/40

USPC · US Patent Classification

536/23.1536/25.42

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Pendency

2.2 y

817 days filing → grant

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non-final + final

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no RCE

Examiner

Ganapathy Krishnan

art unit 1673 · TC 1600

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1 priority documents

›Priority documents — 1

Type	Document	Date
related publication	US 20130289257 A1	31 Oct 2013

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