A method of producing docosahexaenoic acid (DHA), comprising:nculturing a heterotrophic microalga in a culture medium, wherein the culture medium comprises:nchloride ions at a concentration of less than or equal to 3 g/L;potassium ions at a concentration of 0.95 g/L to 10 g/L;wherein the culture medium further comprises sodium ions, wherein a ratio of sodium ions to potassium ions is less than or equal to 27:1; andrecovering DHA-containing lipid from the microalga, wherein the microalga produces at least 0.04 g DHA per 109 cells.
›2.↳ 1The method of claim 1, wherein the concentration of chloride ions is less than or equal to 2 g/L.d2
The method of claim 1, wherein the concentration of chloride ions is less than or equal to 2 g/L.
›3.↳ 1The method of claim 1, wherein the concentration of chloride ions is less than or equal to 1 g/L.d2
The method of claim 1, wherein the concentration of chloride ions is less than or equal to 1 g/L.
›4.↳ 1The method of claim 1, wherein the concentration of chloride ions is less than or equal to 0.3 g/L.d2
The method of claim 1, wherein the concentration of chloride ions is less than or equal to 0.3 g/L.
›5.↳ 1The method of claim 1, wherein the concentration of potassium ions is 0.95 g/L to 4 g/L.d2
The method of claim 1, wherein the concentration of potassium ions is 0.95 g/L to 4 g/L.
›6.↳ 1The method of claim 1, wherein the concentration of potassium ions is 0.95 g/L to 3 g/L.d2
The method of claim 1, wherein the concentration of potassium ions is 0.95 g/L to 3 g/L.
›7.↳ 1The method of claim 1, wherein the concentration of potassium ions is 0.95 g/L to 2.4 g/L.d2
The method of claim 1, wherein the concentration of potassium ions is 0.95 g/L to 2.4 g/L.
›8.↳ 1The method of claim 1, wherein the concentration of potassium ions is 0.95 g/L to 2 g/L.d2
The method of claim 1, wherein the concentration of potassium ions is 0.95 g/L to 2 g/L.
›9.↳ 1The method of claim 1, wherein the sodium ions are at a concentration of 1 g/L to 8 g/L.d2
The method of claim 1, wherein the sodium ions are at a concentration of 1 g/L to 8 g/L.
›10.↳ 1The method of claim 1, wherein the sodium ion is ions are at a concentration of 1 g/L to 8 g/L, and wherein the pH is less than or equal to 5.d2
The method of claim 1, wherein the sodium ion is ions are at a concentration of 1 g/L to 8 g/L, and wherein the pH is less than or equal to 5.
›11.↳ 1The method of claim 1, wherein the sodium ion is ions are at a concentration of 1.5 g/L to 5 g/L, and wherein the pH is 4.5.d2
The method of claim 1, wherein the sodium ion is ions are at a concentration of 1.5 g/L to 5 g/L, and wherein the pH is 4.5.
›12.↳ 1The method of claim 1, wherein a source of potassium comprises potassium sulfate.d2
The method of claim 1, wherein a source of potassium comprises potassium sulfate.
›13.↳ 1The method of claim 1, wherein the microalga produces at least 0.10 g DHA per 109 cells.d2
The method of claim 1, wherein the microalga produces at least 0.10 g DHA per 109 cells.
›14.↳ 1The method of claim 1, wherein the microalga produces at least 0.20 g DHA per 109 cells.d2
The method of claim 1, wherein the microalga produces at least 0.20 g DHA per 109 cells.
›15.↳ 1The method of claim 1, wherein the ratio of sodium ions to potassium ions is less than or equal to 15:1.d2
The method of claim 1, wherein the ratio of sodium ions to potassium ions is less than or equal to 15:1.
›16.↳ 1The method of claim 1, wherein the ratio of sodium ions to potassium ions is less than or equal to 4:1.d2
The method of claim 1, wherein the ratio of sodium ions to potassium ions is less than or equal to 4:1.
›17.↳ 1The method of claim 1, further comprising recovering DHA from the DHA-containing lipid.d2
The method of claim 1, further comprising recovering DHA from the DHA-containing lipid.
›18.↳ 1The method of claim 1, wherein the microalga is of the genus Dinophyceae.d2
The method of claim 1, wherein the microalga is of the genus Dinophyceae.
›19.↳ 1The method of claim 1, wherein the microalga is of the genus Crypthecodinium.d2
The method of claim 1, wherein the microalga is of the genus Crypthecodinium.
›20.↳ 1The method of claim 1, wherein the microalga is of the species Crypthecodinium cohnii.d2
The method of claim 1, wherein the microalga is of the species Crypthecodinium cohnii.
