The present invention relates to polynucleotides from Ostreococcus lucimarinus which code for desaturases and elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides, and to the polypeptides encoded by the polynucleotides. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions.

Claims

23 · 3 independent · depth 4

23 granted claims

›1.independentAn isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:n(a) the nucleic acid sequence of SEQ ID NO: 13;(b…+17

›2.↳ 1The polynucleotide of claim 1, wherein the polynucleotide consists of RNA or DNA.d2

›3.↳ 1A vector comprising the polynucleotide of claim 1.d2+4

›6.↳ 1A host cell comprising the polynucleotide of claim 1.d2+3

›11.↳ 1A method of generating a polypeptide with Î”6-desaturase activity, comprising:n(a) expressing the polynucleotide of claim 1 in a host cell; and(b) obt…d2

›12.↳ 1A polypeptide which is encoded by the polynucleotide of claim 1.d2

›13.↳ 1A transgenic, nonhuman organism comprising the polynucleotide of claim 1, wherein said organism is a plant or a microorganism.d2

›17.↳ 1The polynucleotide of claim 1, wherein the polynucleotide comprises a nucleic acid sequence selected from the group consisting of:n(a) the nucleic aci…d2+1

›19.↳ 1A transgenic plant, plant cell, or plant part comprising the polynucleotide of claim 1.d2

›20.↳ 1A transgenic microorganism comprising the polynucleotide of claim 1.d2

›14.independentA process for the production of a substance which has the structure shown in the general formula I hereinbelow+2

›21.independentAn isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:n(a) the nucleic acid sequence of SEQ ID NO: 13;(b…+1

Classifications

10 codes

IPC · International Patent Classification

Section A — Human necessities

A01H5/00

Section C — Chemistry; metallurgy

C07H21/04
C12N1/20
C12N15/82

USPC · US Patent Classification

800/298435/252.3800/281536/23.2435/320.1435/419

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5.8 y

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Elizabeth McElwain

art unit 1638 · TC 1600

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1 priority documents

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Type	Document	Date
related publication	US 20100088776 A1	8 Apr 2010

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