Compositions and methods for improved protein production

A Trichoderma reesei filamentous fungus having a mutation or deletion of part or all of a gene having the sequence of SEQ ID NO: 4, and said mutation or deletion results in the enhanced production of a desired polypeptide compared to the parent T. reesei filamentous fungus.

A method for the production of a heterologous protein in a transformed Trichoderma reesei host cell comprising:ngrowing a T. reesei host cell comprising a nucleic acid encoding said heterologous protein wherein said host cell contains a mutation or deletion of part or all of a gene having the sequence of SEQ ID NO: 4, wherein said mutation or deletion results in the enhanced production of the heterologous protein compared to a parent filamentous fungus, and wherein said growing of said host is under conditions suitable for the expression of said heterologous protein.

An isolated nucleotide sequence having a truncation or mutation of the sequence of SEQ ID NO: 4, wherein the truncation or mutation reduces or eliminates expression of SEQ ID NO: 4 and wherein said nucleotide sequence is capable of homologous recombination with a wild-type gene having the nucleotide sequence of SEQ ID NO: 4.