The object is to provide a transformant which can produce a heterologous protein having a structurally controlled O-linked sugar chain having an O-Man-Gal disaccharide structure, a method for producing the transformant by using Schizosaccharomyces pombe as the host, and provide a host for producing the transformant and a method for producing an O-glycosylated heterologous protein. An Schizosaccharomyces pombe host having no omh1 gene or an inactivated omh1 gene in its chromosomes for producing an O-glycosylated heterologous protein having an O-linked sugar chain having an O-Man-Gal disaccharide structure by expression of the heterologous protein by a genetic engineering technique and subsequent glycosylation of the expressed heterologous protein. A transformant from the host, a method for producing the transformant and a method for producing an O-glycosylated heterologous protein by using the transformant.

Claims

11 · 1 independent · depth 3

11 granted claims

›1.independentA Schizosaccharomyces pombe host having no omh1 gene or an inactivated omh1 gene and comprising a heterologous gene, wherein the heterologous gene exp…+10

›2.↳ 1The Schizosaccharomyces pombe host according to claim 1, wherein the heterologous gene is a recombinant fusion further comprising a gene encoding a se…d2

›3.↳ 1The Schizosaccharomyces pombe host according to claim 1, wherein the heterologous protein, in wild type form, produced by the transformant is an O-gly…d2

›4.↳ 1A method for producing the Schizosaccharomyces pombe host according to claim 1, the method comprising introducing the heterologous gene into the Schiz…d2+2

›7.↳ 1A method for producing an O-glycosylated heterologous protein, the method comprising culturing the Schizosaccharomyces pombe host of claim 1 to produc…d2+2

›10.↳ 1The Schizosaccharomyces pombe host according to claim 1, which has no omh1 gene.d2

›11.↳ 1The Schizosaccharomyces pombe host according to claim 1, which has an inactivated omh1 gene.d2

Classifications

24 codes

IPC · International Patent Classification

Section C — Chemistry; metallurgy

C12N15/00
C12P1/00
C12P19/44
C12N15/09
C12P19/00
C12N15/74
C12P19/04
C12P21/06
C12P19/18
C12N1/00

USPC · US Patent Classification

435/41435/254.11435/101435/254.1435/97435/476435/69.4435/471435/74435/440435/69.1435/477435/72435/243

Claim changes

Soon

Coming soonHow the claims changed between publication and grant

See which claims were amended, added or cancelled during examination, with every added and removed word marked.

AmendedAddedCancelledUnchanged

The published claims of this patent are not paired with the granted ones in what we hold.

File wrapper

Pendency

1.7 y

607 days filing → grant

Office actions

after a restriction

Responses

1 RCE

Interviews

examiner interview summaries

Examiner

Addison D Ault

art unit —

Law firm

Law firm · Log in to unlock

Citations: 13 back · 0 forward

See the full prosecution history — every USPTO and applicant action on this file, in order.

Term & fees

See the term timeline — pendency span, in-force span, the maintenance fees paid and both computed expiry dates.

Priority chain

1 priority documents

›Priority documents — 1

Type	Document	Date
related publication	US 20110287481 A1	24 Nov 2011

Validity challenges

See the validity challenges on record — reexaminations, IPRs and PGRs, with their institution decisions and outcomes.

Citations

See every patent this one cites and every patent that cites it back — publication, assignee, and how each one was found.