Estimation of change in bone mineral density and diagnosis of osteoporosis

A method for estimation of change in bone mineral density, comprising the steps of: (1) bringing a blood sample into contact with a solid carrier on the surface of which an anti soluble interleukin-6 receptor (sIL-6) antibody (first antibody) has been immobilized; (2) bringing into contact with the solid carrier from the step (1) (i) an anti sIL-6R antibody (second antibody) bound to a detectable marker, or (ii) an anti sIL-6R antibody (second antibody) capable of binding to a detectable marker, followed by bringing a detectable marker into contact with the anti sIL-6R antibody; and (3) measuring the amount of the detectable marker immobilized on the solid carrier, wherein the amount of the immobilized detectable marker relates to an amount of sIL-6R in the blood sample, and the amount of sIL-6R relates to the bone mineral density.

A method for estimation of change in bone mineral density, comprising the steps of: (1) contacting a blond sample with anti sIL-6R antibody (second antibody) bound to a detectable marker, thereby forming a mixture; (2) bringing the mixture from the step (1) into contact with a solid carrier on the surface of which an anti IL-6R antibody (first antibody) has been immobilized; and (3) measuring the amount of the detectable marker immobilized on the solid carrier, wherein the amount of the immobilized detectable marker relates to an amount of sIL-6R in the blood sample, and the amount of sIL-6R relates to the bone mineral density.

A kit for estimating change in bone mineral density or diagnosis of osteoporosis, comprising: (1) an anti-sIL-6R antibody immobilized to a solid carrier, and (2) an anti-sIL-6R antibody which is bound to a detectable marker which is capable of binding a detectable marker.

A method for estimation of change in bone mineral density, comprising the steps of: (1) bringing a blood sample, an anti sIL-6R antibody (second antibody), and a detectable marker into contact with a solid carrier on the surface of which an anti sIL-6R antibody (first antibody) has been immobilized, wherein the anti sIL-6R antibody and the detectable marker are optionally bound together before mixing; and (2) measuring the amount of the detectable marker immobilized on the solid carrier, wherein the amount of the immobilized detectable carrier relates to an amount of sIL-6R in the blood sample, and the amount of sIL-6R in the blood sample relates to the bone mineral density.

A method for estimation of change in bone mineral density, comprising the steps of: (1) contacting a blood sample with a marker-bound sIL-6R, thereby forming a mixture; (2) bringing the mixture into contact with a solid carrier on the surface of which an anti sIL-6R antibody has been immobilized; and (3) measuring the amount of the marker immobilized on the solid carrier, wherein the amount of the immobilized marker reversely reflects an amount of sIL-6R in the sample, and the amount of sIL-6R relates to the bone mineral density.

A method for diagnosis of osteoporosis, comprising the steps of: (1) bringing a blood sample into contact with a solid carrier on the surface of which an anti sIL-6R antibody (first antibody) has been immobilized; (2) bringing Into contact with the solid carrier from the step (1) (i) an anti sIL-6R antibody (second antibody) bound to a detectable marker, or (ii) an anti sIL-6R antibody (second antibody) capable of binding to a detectable marker, followed by bringing a detectable marker into contact with the anti sIL-6R antibody (second antibody); and (3) measuring the amount of the detectable marker immobilized on the solid carrier, wherein the amount of the immobilized detectable carrier relates to an amount of sIL-6R in the blood sample, and the amount of sIL-6R relates to the diagnosis of the osteoporosis.

A method for diagnosis of osteoporosis, comprising the steps of: (1) contacting a blood sample suspected to contain sIL-6R and an ant sIL-6R antibody (second antibody) bound to a detectable marker, thereby forming a mixture; (2) bringing the mixture from the step (1) into contact with a solid carrier on the surface of which an anti IL-6R antibody (first antibody) has been immobilized; and (3) measuring the amount of the detectable marker immobilized on the solid carrier, wherein the amount of the immobilized detectable marker relates to an amount of sIL-6R in the blood sample, and the amount of sIL-6R relates to the diagnosis of the osteoporosis.

A method for diagnosis of osteoporosis, comprising the steps of: (1) bringing a blood sample, an anti sIL-6R antibody (second antibody), and a detectable marker into contact with a solid carrier on the surface of which an anti sIL-6R antibody, and (first antibody) has been immobilized, wherein the anti sIL-6R antibody and the detectable marker are optionally bound together before mixing; and (2) measuring the amount of the detectable marker immobilized on the solid carrier, wherein the amount of the immobilized detectable carrier relates to an amount of sIL-6R in the blood sample, and the amount of sIL-6R relates to the diagnosis of the osteoporosis.

A method for diagnosis of osteoporosis, comprising the steps of: (1) contacting a blood sample and a marker-bound sIL-6R, thereby forming a mixture; (2) bringing the mixture into contact with a solid carrier on the surface of which an anti IL-6R antibody has been immobilized; and (3) measuring the amount of the marker immobilized on the solid carrier, wherein the amount of the immobilized marker reversely reflects an amount of sIL-6R in the sample, and the amount of sIL-6R relates to the diagnosis of osteoporosis.