A process for the enzymatic conversion of glucose to gluconic acid comprising providing a solution of glucose; and adding to the solution, in the presence of an oxygen source, from about 25 to about 30 glucose oxidase units of soluble glucose oxidase/gram dissolved solids (ds.) of glucose in the solution and at least 1200 catalase units of soluble catalase/gram dissolved solids (ds.) of glucose in the solution.
›2.↳ 1The process of claim 1, further comprising the solution of glucose having from about 25% weight/weight (w/w) ds. of glucose to about 60% (w/w) ds. of …d2
The process of claim 1, further comprising the solution of glucose having from about 25% weight/weight (w/w) ds. of glucose to about 60% (w/w) ds. of glucose.
›3.↳ 1The process of claim 1, further comprising the solution of glucose having from about 30% (w/w) ds. of glucose to about 50% (w/w) ds. of glucose.d2
The process of claim 1, further comprising the solution of glucose having from about 30% (w/w) ds. of glucose to about 50% (w/w) ds. of glucose.
›4.↳ 1The process of claim 1, wherein about 27 GOU of glucose oxidase/gram ds. of glucose is added to the solution.d2
The process of claim 1, wherein about 27 GOU of glucose oxidase/gram ds. of glucose is added to the solution.
›5.↳ 1The process of claim 1, wherein at least about 1279 CU of catalase/gram ds. of glucose is added to the solution.d2
The process of claim 1, wherein at least about 1279 CU of catalase/gram ds. of glucose is added to the solution.
›6.↳ 1The process of claim 1, wherein at least about 1559 CU of catalase/gram ds. of glucose is added to the solution.d2
The process of claim 1, wherein at least about 1559 CU of catalase/gram ds. of glucose is added to the solution.
›7.↳ 1The process of claim 1, wherein at least about 1999 CU of catalase/gram ds. of glucose is added to the solution.d2
The process of claim 1, wherein at least about 1999 CU of catalase/gram ds. of glucose is added to the solution.
›8.↳ 1The process of claim 1, wherein the glucose oxidase and the catalase are added to the solution of glucose in two equal doses, the first dose being add…d2
The process of claim 1, wherein the glucose oxidase and the catalase are added to the solution of glucose in two equal doses, the first dose being added at the start of the reaction and the second dose being added halfway through the total intended time of the reaction.
›9.↳ 1The process of claim 1, wherein the catalase is naturally produced by a strain of the species Aspergillus niger.d2
The process of claim 1, wherein the catalase is naturally produced by a strain of the species Aspergillus niger.
›10.↳ 1The process of claim 1, wherein the solution of glucose is maintained at a pH of from about 5 to about 7.d2+1
The process of claim 1, wherein the solution of glucose is maintained at a pH of from about 5 to about 7.
›11.↳ 10The process of claim 10, wherein the solution of glucose is maintained at a pH of about 6.d3
The process of claim 10, wherein the solution of glucose is maintained at a pH of about 6.
›12.↳ 1The process of claim 1, wherein the temperature of the solution of glucose is maintained at from about 25.degree. C. to about 40.degree. C.d2+1
The process of claim 1, wherein the temperature of the solution of glucose is maintained at from about 25.degree. C. to about 40.degree. C.
›13.↳ 12The process of claim 12, wherein the temperature of the solution of glucose is maintained at from about 30.degree. C. to about 35.degree. C.d3
The process of claim 12, wherein the temperature of the solution of glucose is maintained at from about 30.degree. C. to about 35.degree. C.
›14.↳ 1The process of claim 1, wherein the presssure of the solution of glucose is maintained at about 1 bar.d2
The process of claim 1, wherein the presssure of the solution of glucose is maintained at about 1 bar.
›15.↳ 1The process of claim 1, further including maintaining an air flow through the solution during the reaction of about 1 volume gas per volume of reactio…d2
The process of claim 1, further including maintaining an air flow through the solution during the reaction of about 1 volume gas per volume of reaction medium per minute (vvm).
›18.↳ 1The process of claim 1, wherein the catalase is naturally produced by a microbial or mammalian source.d2
The process of claim 1, wherein the catalase is naturally produced by a microbial or mammalian source.
›19.↳ 1The process of claim 1, wherein the catalase is naturally produced by a microbial source.d2
The process of claim 1, wherein the catalase is naturally produced by a microbial source.
›20.↳ 1The process of claim 1, wherein the catalase is naturally produced by a strain of Micrococcus lysodeikticus.d2
The process of claim 1, wherein the catalase is naturally produced by a strain of Micrococcus lysodeikticus.
