Peptides for HIV-1 detection

A polypeptide having a point mutation in the HIV-1 sub-type B IDR at position 604.

A polypeptide having a point mutation in the HIV-1 sub-type B IDR at position 610.

A polypeptide having two single point mutations in the HIV-1 sub-type B IDR at positions 604 and 610.

Polypeptide SEQUENCE I.D. No. 2.

Polypeptide SEQUENCE I.D. No. 3.

Polypeptide SEQUENCE I.D. No. 4.

An immunoassay to detect the presence of HIV antibodies in a test sample, comprising: a) contacting said test sample with a solid phase to which has been attached an HIV-1 polypeptide having a point mutation between positions 593 and 611 to form a first mixture, and incubating said first mixture for a time and for conditions sufficient to form polypeptide/antibody complexes; b) contacting said polypeptide/antibody complexes with an indicator reagent comprising a member of a specific binding pair attached to a signal generating compound capable of generating a measureable signal to form a second mixture, and incubating said second mixture for a time and for conditions sufficient to form polypeptide/antibody/indicator reagent complexes; and c) determing the presence of HIV antibodies in said test sample by detecting the measureable signal.

In an immunoassay for detecting HIV antibody in a test sample comprising contacting said test sample with an HIV-1 polypeptide and detecting the presence of said antibody, wherein the improvement comprises utilizing a polypeptide having at least one point mutation between positions 593 and 611 of the HIV-1 gp160 sequence.

A dignostic test kit capable of detecting HIV antibodies comprising a container containing a polypeptide having a sequence selected from the group consisting of SEQUENCE I.D. No. 2, SEQUENCE No. 3 and SEQUENCE No. 4 .