Osteogenic proteins

The osteogenic protein of claim 1 having a molecular weight of about 30 kD when oxidized as determined by comparison to molecular weight standards in SDS-polyacrylamide gel.

The osteogenic protein of claim 1 having a molecular weight of about 27 kD as determined by comparison to molecular weight standards in SDS-polyacrylamide gel electrophoresis.

The protein of claim 1 or 4 further characterized by being unglycosylated.

The protein of claim 1 comprising the amino acid sequences: ##STR24## wherein each X independently represents an amino acid.

The protein of claim 1 comprising the amino acid sequences: ##STR25## wherein each X independently represents an amino acid.

The protein of claim 1 comprising the amino acid sequence: ##STR26##

The protein of claim 1 comprising the amino acid sequences: ##STR27##

The protein of claim 1 comprising the product of expression of a DNA in a procaryotic host cell.

The protein of claim 1 produced by expression in a mammalian cell line.

The protein of claim 1 having a half-maximum bone forming activity of at least about 20-25 ng per 25 mg of implant.

The protein of claim 1 comprising the amino acid sequences: ##STR28## wherein X.sub.1 =(K or R); X.sub.2 =(H,R, or K); X.sub.3 =(P, S, E or Q); X.sub.4 --(Y, K or F); X.sub.5 =(D,S, or E); X.sub.6 =(R, S, K or A); X.sub.7 =(V, L, or I); X.sub.8 =(N, Q, D or S); X.sub.9 =(D, E or N); X.sub.10 =(I or V); X.sub.11 =(I or V); X.sub.12 =(A or S); X.sub.13 =(P, E, L or K); X.sub.14 =(Y or F); X.sub.15 =(H or D); X.sub.16 =(F, Y or N); X.sub.17 =(H, E or S); X.sub.18 =(E or A); X.sub.19 =(P, A or Q); X.sub.20 =(F or Y); X.sub.21 =(L, M or I); X.sub.22 =(A, P or T); X.sub.23 =(D, E or K); X.sub.24 =(H or S); X.sub.25 =(L, M or F); X.sub.26 =(N or K); X.sub.27 =(S, A or P); X.sub.28 =(T or S); X.sub.29 =(I, V, or T); X.sub.30 =(V, I or L); X.sub.31 =(T or S); X.sub.32 =(L or I); X.sub.33 =(N, H or R); X.sub.34 =(S, A, F or N); X.sub.35 =(V or I); X.sub.36 =(P or S); X.sub.37 =(G or E); X.sub.38 =(K, Q, T or S); X.sub.39 =(I or V); X.sub.40 =(K or E); X.sub.41 =(A, P or S); X.sub.42 =(V or A); X.sub.43 =(T or E); X.sub.44 =(E, Q or K); X.sub.45 =(L or M); X.sub.46 =(S N or D); X.sub.47 =(a, S or P); X.sub.48 =(I, L or V); X.sub.49 =(S or A); X.sub.50 =(M, I or V); X.sub.51 =(Y or F); X.sub.52 =(L, F or Y); X.sub.53 =(D or N); X.sub.54 =(E, D or N); X.sub.55 =(N or Q); X.sub.56 =(E, D, S or K); X.sub.57 =(N or K); X.sub.58 =(V or I); X.sub.59 =(K or R); X.sub.60 =(N, K or H); X.sub. 61 =(Q, E, R or P); X.sub.62 =(D, E or N); X.sub.63 =(V or T); X.sub.64 =(E, D or R); X.sub.65 =(G, A, S or E); X.sub.66 =(G or H); and X.sub.67 =(R or H).

The protein of claim 1 comprising the amino acid sequences: ##STR29## wherein X.sub.1 =(Y, K or F); X.sub.2 =(D, S or E); X.sub.3 =(R, S, K or A); X.sub.4 =(V, L, or I); X.sub.5 =(N, Q, D or S); X.sub.6 =(D, E or N); X.sub.7 =(I or V); X.sub.8 =(I or V); X.sub.9 =(A or S); X.sub.10 (P, E, L or K); X.sub.11 =(Y or F); X.sub.12 =(H or D); X.sub.13 =(F, Y or N); X.sub.14 =(H, E or S); X.sub.15 =(E or A); X.sub.16 =(P, A or Q); X.sub.17 =(F or Y); X.sub.18 =(L, M or I); X.sub.19 =(A, P or T); X.sub.20 =(D, E or K); X.sub.21 =(H or S); X.sub.22 =(L, M or F); X.sub.23 =(N or K); X.sub.24 =(S, A or P); X.sub.25 =(T or S); X.sub.26 =(I, V, or T); X.sub.27 =(V, I or L); X.sub.28 =(T or S); X.sub.29 =(L or I); X.sub.30 =(N, H or R); X.sub.31 =(S, A, F or N); X.sub.32 =(V or I); X.sub.33 =(P or S); X.sub.34 =(G or E); X.sub.35 =(K, Q, T or S); X.sub.36 =(I or V); X.sub.37 =(K or E); X.sub.38 =(A, P or S); X.sub.39 =(V or A); X.sub.40 =(T or E); X.sub.41 =(E, Q or K); X.sub.42 =(L or M); X.sub.43 =(S N or D); X.sub.44 =(A, S or P); X.sub.45 =(I, L or V); X.sub.46 =(S or A); X.sub.47 =(M, I or V); X.sub.48 =(Y or F); X.sub.49 =(L, F or Y); X.sub.50 =(D or N); X.sub.51 =(E, D or N); X.sub.52 =(N or Q); X.sup.53 =(E, D, S or K); X.sub.54 =(N or K); X.sub.55 =(V or I); X.sub.56 =(K or R); X.sub.57 =(N, K or H); X.sub.58 =(Q, E, R or P); X.sub.59 =(D, E or N); X.sub.60 =(V or T); X.sub.61 =(E, D or R); X.sub.62 =(G, A, S or E); X.sub.63 =(g or H); and X.sub.64 =(R or H).